ORF » Species Summary » Zea mays » LOC100191474 cDNA ORF clone

LOC100191474 cDNA ORF clone, Zea mays

The following LOC100191474 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100191474 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OZa54077 NM_001164334.2
Latest version!		 Zea mays topoisomerase I (LOC100191474), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OZa54077 XM_020551257.2
Latest version!		 Zea mays topoisomerase I (Top1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OZa54077 XM_020551257.1 Zea mays topoisomerase I (Top1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC100191474 ( NM_001164334.2 ) cDNA ORF clone, Zea mays -> NP_001157806.2 Zea mays topoisomerase I (LOC100191474), mRNA.
    Price & Availability↑
    CloneID OZa54077
    Clone ID Related Accession (Same CDS sequence) XM_020551257.2 , XM_020551257.1 , NM_001164334.2
    Accession Version NM_001164334.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1552147200000
    Organism Zea mays
    Product topoisomerase I
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from LPUQ01000805.1 and BT053789.1. On Feb 12, 2017 this sequence version replaced NM_001164334.1. ##Evidence-Data-START## Transcript exon combination :: GQ181129.1, SRR3147024.12015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01096209 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTTG TCAACCCAAC CAACCTTGCT ATTGACGACG ATGACGACGA TACTCCCATT 
    TCATTCAAGA GGACGAGTAA CTCTACGAAG ACTGCCAGGC CCACCCCTTC CAAGCATGAG 
    GGGTCGTCAG GAGGTAATGG TGCACCTGTT AGGATCCCCA AACCTGTGTC TCCCAATCCG 
    CAGAAGAACG GAGCCACCGG GGCTTCAAGG CCGTTGCATA TGAAGCCACC GTCATCCAGT 
    CCAAACCATC GTCCTTCTGG ATCTGGCCAG CCAAACAGTT TGGCACAACA TAGTTCAAAG 
    GCTAATAGCA TTGACATGAG TAAACTGAAA AGACCTCTCG TCAAAGATGA GAGATCTGAT 
    GACTCAGATG ACGAGGTGCC GATTGGGTTA AGGAGAAAGG TTGAGGAGAA GAAGTTGAAG 
    GGAGTTGATA CTCGGGTTCA GAAGACAGAT GACTCAGATG ACGATGATAA GCCATTGGGT 
    CTCAAGATCA ACTCATCCAG AATGTCTTCC AACAGAGTGA ATAAGCCTGT TCTGCAGAGA 
    ACTGTACCAA AGGTTGAGCA GCCTGATGAA GATTCAGATG ATGATAAACC ATTGGCAAGC 
    AGGTTGCCCA GTAATGCCGG TCCAAAAAGT GGAGGTGATG TCTCTGAGGA TTCAGAGGAT 
    GAGAAGCCAC TGGCTGCCAG ATTTTCAAAA GTTGCTGGTA GTGGAAACCC AAAAATAGCT 
    TCTTCCAGCA AAGGGTTAAC CAGTGATACA AATGGCCAAC AAAGTTTGGG TAAAAGGCCT 
    CTAGACAATA GTAATCAAAC AAGTTCAGCT CTTAAGAAGG CTAAACCTTC AAACGTTTCA 
    GCTTCTGCAA TCGTTAGAAG AGAAATCAAG ACAGATGACA ACACACCTCT TGCACAAAGG 
    CTGAAAATGG GAGAGTCTTC GAAAGGCAAG TCATCTGCAA AGAGTGTCTT TAAGAAGAGT 
    CCTGCTTCTG TGAAGAAGGA CATCAAGAAA ATGAAAGGGA AAGGGAAATT AAAGAAGAAC 
    ATGAAAAATT CTCAGTTCTC AAAGATAATG AAGGTCCCCC CTGGATCTGG TGGAGGAAAG 
    AAATGGACTA CCTTGGAGCA CAATGGTGTT ATTTTCCCTC CCCCATACAA GCCTCATGGT 
    GTCAAAATGC TTTACAATGG GAAACCTATC GATCTGACAC CAGAACAAGA GGAGGTTGCA 
    ACAATGTTTG CTGTTATGAA AGACACAGAC TATGCGGCCA AGCCAACATT CATTGATAAC 
    TTTTTCACTG ATTGGAGAAA AGTTCTTGGT AAAAATCATA TTATTAAGAA CTTCGAGCTT 
    TGTGATTTCA CACCAATATA TGAATGGCAC CTCAGAGAGA AGGAGAGGAA GAAGCAGATG 
    ACAACAGAGG AGAAAAAAAC ATTGCGGGAA GACAAATTGA AACAAGAGGA GAAGTACATG 
    TGGGCTGTTG TAGATGGTGT TAAAGAAAAG GTCGGAAATT TCAGAGTAGA ACCGCCTGGC 
    TTGTTCAGGG GACGTGGAGA ACATCCAAAG ATGGGGAAAC TTAAGCGCCG CATTCTACCA 
    AGTGATATTA CAATAAATAT TGGAAAAGGA GCTCCAGTCC CTGAGTGCCC GATACCTGGA 
    GAAAGTTGGA AAGAAGTCAA ACATGACAAT ACTGTTACAT GGTTGGCCTT TTGGAACGAT 
    CCAATTAACC CAAAGGATTT CAAGTATGTT TTCCTGGCAG CAAGCAGCTC ACTGAAGGGG 
    CAAAGTGACA AAGAGAAGTA TGAGAAGTCC AGAAAATTGA AGGGTCATAT ACAAAGAATT 
    CGTGAGAATT ATACAAAAGA TTTCAAAAAC AAAGATGTGA CGAGGAGGCA AATAGCTGTG 
    GCAACATACC TTATCGATAA ACTAGCCCTT AGGGCTGGCA ATGAAAAGGA CGACGATGAA 
    GCTGATACTG TTGGTTGCTG TACGCTGAAG GTTGATAATG TTACATGTAT GCCTCCAAAT 
    CAGATTCAGT TTGACTTTCT GGGTAAAGAT TCAATAAGAT ACTTTAACAC CGTAGAGGTT 
    GAAGAGCTTG TGTACAAGGC AATTGAGGGT TTCCGTGCTG GAAAGAAGCC AGGACAAGAT 
    CTATTTGACA AGATTGATAC ATCTAGGCTA AATGCTCACT TGAAGGATCT AATGCCTGGG 
    CTCACTGCAA AAGTGTTCCG TACATATAAT GCTTCCATAA CATTGGACGG AATCTTGCAT 
    GAAGAAACAG AAGATGGAAC TGTTTTAGAA AAAATCGCTG TCTATCAGCG GGCAAACAAA 
    GAGGTTGCCA TCATCTGTAA CCATCAGCGT AGTGTCTCGA AATCACATGA GTCCCAGATG 
    ACTAGAATGA ACGAAAAGAT TGATGAGTTG AAGTCCCAGA TGGAGGAGCT GAAAGCAGAC 
    TTGAACAAAG CAAAGAAAGG GAAGCCTCTA GGCTATGATT CAGAGGGGAA GCCAAAGAGG 
    AACTTGGCTC CTGAAGCGAT TCAAAAGAAG GTTGCAACAC TTGAAGGCAA GATAGAGAAG 
    ATGGAGATGG ATAAGAGGAT AAAAGAGGAT CTGAAGACAG TTGCGCTTGG AACGTCAAAG 
    ATCAACTATC TTGATCCTAG AATAACCGTG GCATGGTGCA AACGCCATGA AGTCCCTATT 
    GAGAAGATTT TCAACAAATC TCTCCTCGCG AAATTTGTAT GGGCAATGGA CGTTGACCCG 
    GAATTCAGAT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157806.2
    CDS223..2937
    Translation

    Target ORF information:

    RefSeq Version NM_001164334.2
    Organism Zea mays
    Definition Zea mays topoisomerase I (LOC100191474), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001164334.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTTG TCAACCCAAC CAACCTTGCT ATTGACGACG ATGACGACGA TACTCCCATT 
    TCATTCAAGA GGACGAGTAA CTCTACGAAG ACTGCCAGGC CCACCCCTTC CAAGCATGAG 
    GGGTCGTCAG GAGGTAATGG TGCACCTGTT AGGATCCCCA AACCTGTGTC TCCCAATCCG 
    CAGAAGAACG GAGCCACCGG GGCTTCAAGG CCGTTGCATA TGAAGCCACC GTCATCCAGT 
    CCAAACCATC GTCCTTCTGG ATCTGGCCAG CCAAACAGTT TGGCACAACA TAGTTCAAAG 
    GCTAATAGCA TTGACATGAG TAAACTGAAA AGACCTCTCG TCAAAGATGA GAGATCTGAT 
    GACTCAGATG ACGAGGTGCC GATTGGGTTA AGGAGAAAGG TTGAGGAGAA GAAGTTGAAG 
    GGAGTTGATA CTCGGGTTCA GAAGACAGAT GACTCAGATG ACGATGATAA GCCATTGGGT 
    CTCAAGATCA ACTCATCCAG AATGTCTTCC AACAGAGTGA ATAAGCCTGT TCTGCAGAGA 
    ACTGTACCAA AGGTTGAGCA GCCTGATGAA GATTCAGATG ATGATAAACC ATTGGCAAGC 
    AGGTTGCCCA GTAATGCCGG TCCAAAAAGT GGAGGTGATG TCTCTGAGGA TTCAGAGGAT 
    GAGAAGCCAC TGGCTGCCAG ATTTTCAAAA GTTGCTGGTA GTGGAAACCC AAAAATAGCT 
    TCTTCCAGCA AAGGGTTAAC CAGTGATACA AATGGCCAAC AAAGTTTGGG TAAAAGGCCT 
    CTAGACAATA GTAATCAAAC AAGTTCAGCT CTTAAGAAGG CTAAACCTTC AAACGTTTCA 
    GCTTCTGCAA TCGTTAGAAG AGAAATCAAG ACAGATGACA ACACACCTCT TGCACAAAGG 
    CTGAAAATGG GAGAGTCTTC GAAAGGCAAG TCATCTGCAA AGAGTGTCTT TAAGAAGAGT 
    CCTGCTTCTG TGAAGAAGGA CATCAAGAAA ATGAAAGGGA AAGGGAAATT AAAGAAGAAC 
    ATGAAAAATT CTCAGTTCTC AAAGATAATG AAGGTCCCCC CTGGATCTGG TGGAGGAAAG 
    AAATGGACTA CCTTGGAGCA CAATGGTGTT ATTTTCCCTC CCCCATACAA GCCTCATGGT 
    GTCAAAATGC TTTACAATGG GAAACCTATC GATCTGACAC CAGAACAAGA GGAGGTTGCA 
    ACAATGTTTG CTGTTATGAA AGACACAGAC TATGCGGCCA AGCCAACATT CATTGATAAC 
    TTTTTCACTG ATTGGAGAAA AGTTCTTGGT AAAAATCATA TTATTAAGAA CTTCGAGCTT 
    TGTGATTTCA CACCAATATA TGAATGGCAC CTCAGAGAGA AGGAGAGGAA GAAGCAGATG 
    ACAACAGAGG AGAAAAAAAC ATTGCGGGAA GACAAATTGA AACAAGAGGA GAAGTACATG 
    TGGGCTGTTG TAGATGGTGT TAAAGAAAAG GTCGGAAATT TCAGAGTAGA ACCGCCTGGC 
    TTGTTCAGGG GACGTGGAGA ACATCCAAAG ATGGGGAAAC TTAAGCGCCG CATTCTACCA 
    AGTGATATTA CAATAAATAT TGGAAAAGGA GCTCCAGTCC CTGAGTGCCC GATACCTGGA 
    GAAAGTTGGA AAGAAGTCAA ACATGACAAT ACTGTTACAT GGTTGGCCTT TTGGAACGAT 
    CCAATTAACC CAAAGGATTT CAAGTATGTT TTCCTGGCAG CAAGCAGCTC ACTGAAGGGG 
    CAAAGTGACA AAGAGAAGTA TGAGAAGTCC AGAAAATTGA AGGGTCATAT ACAAAGAATT 
    CGTGAGAATT ATACAAAAGA TTTCAAAAAC AAAGATGTGA CGAGGAGGCA AATAGCTGTG 
    GCAACATACC TTATCGATAA ACTAGCCCTT AGGGCTGGCA ATGAAAAGGA CGACGATGAA 
    GCTGATACTG TTGGTTGCTG TACGCTGAAG GTTGATAATG TTACATGTAT GCCTCCAAAT 
    CAGATTCAGT TTGACTTTCT GGGTAAAGAT TCAATAAGAT ACTTTAACAC CGTAGAGGTT 
    GAAGAGCTTG TGTACAAGGC AATTGAGGGT TTCCGTGCTG GAAAGAAGCC AGGACAAGAT 
    CTATTTGACA AGATTGATAC ATCTAGGCTA AATGCTCACT TGAAGGATCT AATGCCTGGG 
    CTCACTGCAA AAGTGTTCCG TACATATAAT GCTTCCATAA CATTGGACGG AATCTTGCAT 
    GAAGAAACAG AAGATGGAAC TGTTTTAGAA AAAATCGCTG TCTATCAGCG GGCAAACAAA 
    GAGGTTGCCA TCATCTGTAA CCATCAGCGT AGTGTCTCGA AATCACATGA GTCCCAGATG 
    ACTAGAATGA ACGAAAAGAT TGATGAGTTG AAGTCCCAGA TGGAGGAGCT GAAAGCAGAC 
    TTGAACAAAG CAAAGAAAGG GAAGCCTCTA GGCTATGATT CAGAGGGGAA GCCAAAGAGG 
    AACTTGGCTC CTGAAGCGAT TCAAAAGAAG GTTGCAACAC TTGAAGGCAA GATAGAGAAG 
    ATGGAGATGG ATAAGAGGAT AAAAGAGGAT CTGAAGACAG TTGCGCTTGG AACGTCAAAG 
    ATCAACTATC TTGATCCTAG AATAACCGTG GCATGGTGCA AACGCCATGA AGTCCCTATT 
    GAGAAGATTT TCAACAAATC TCTCCTCGCG AAATTTGTAT GGGCAATGGA CGTTGACCCG 
    GAATTCAGAT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100191474 ( XM_020551257.2 ) cDNA ORF clone, Zea mays -> XP_020406846.1 Zea mays topoisomerase I (Top1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OZa54077
    Clone ID Related Accession (Same CDS sequence) XM_020551257.2 , XM_020551257.1 , NM_001164334.2
    Accession Version XM_020551257.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1513440000000
    Organism Zea mays
    Product topoisomerase I isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024462.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020551257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTTG TCAACCCAAC CAACCTTGCT ATTGACGACG ATGACGACGA TACTCCCATT 
    TCATTCAAGA GGACGAGTAA CTCTACGAAG ACTGCCAGGC CCACCCCTTC CAAGCATGAG 
    GGGTCGTCAG GAGGTAATGG TGCACCTGTT AGGATCCCCA AACCTGTGTC TCCCAATCCG 
    CAGAAGAACG GAGCCACCGG GGCTTCAAGG CCGTTGCATA TGAAGCCACC GTCATCCAGT 
    CCAAACCATC GTCCTTCTGG ATCTGGCCAG CCAAACAGTT TGGCACAACA TAGTTCAAAG 
    GCTAATAGCA TTGACATGAG TAAACTGAAA AGACCTCTCG TCAAAGATGA GAGATCTGAT 
    GACTCAGATG ACGAGGTGCC GATTGGGTTA AGGAGAAAGG TTGAGGAGAA GAAGTTGAAG 
    GGAGTTGATA CTCGGGTTCA GAAGACAGAT GACTCAGATG ACGATGATAA GCCATTGGGT 
    CTCAAGATCA ACTCATCCAG AATGTCTTCC AACAGAGTGA ATAAGCCTGT TCTGCAGAGA 
    ACTGTACCAA AGGTTGAGCA GCCTGATGAA GATTCAGATG ATGATAAACC ATTGGCAAGC 
    AGGTTGCCCA GTAATGCCGG TCCAAAAAGT GGAGGTGATG TCTCTGAGGA TTCAGAGGAT 
    GAGAAGCCAC TGGCTGCCAG ATTTTCAAAA GTTGCTGGTA GTGGAAACCC AAAAATAGCT 
    TCTTCCAGCA AAGGGTTAAC CAGTGATACA AATGGCCAAC AAAGTTTGGG TAAAAGGCCT 
    CTAGACAATA GTAATCAAAC AAGTTCAGCT CTTAAGAAGG CTAAACCTTC AAACGTTTCA 
    GCTTCTGCAA TCGTTAGAAG AGAAATCAAG ACAGATGACA ACACACCTCT TGCACAAAGG 
    CTGAAAATGG GAGAGTCTTC GAAAGGCAAG TCATCTGCAA AGAGTGTCTT TAAGAAGAGT 
    CCTGCTTCTG TGAAGAAGGA CATCAAGAAA ATGAAAGGGA AAGGGAAATT AAAGAAGAAC 
    ATGAAAAATT CTCAGTTCTC AAAGATAATG AAGGTCCCCC CTGGATCTGG TGGAGGAAAG 
    AAATGGACTA CCTTGGAGCA CAATGGTGTT ATTTTCCCTC CCCCATACAA GCCTCATGGT 
    GTCAAAATGC TTTACAATGG GAAACCTATC GATCTGACAC CAGAACAAGA GGAGGTTGCA 
    ACAATGTTTG CTGTTATGAA AGACACAGAC TATGCGGCCA AGCCAACATT CATTGATAAC 
    TTTTTCACTG ATTGGAGAAA AGTTCTTGGT AAAAATCATA TTATTAAGAA CTTCGAGCTT 
    TGTGATTTCA CACCAATATA TGAATGGCAC CTCAGAGAGA AGGAGAGGAA GAAGCAGATG 
    ACAACAGAGG AGAAAAAAAC ATTGCGGGAA GACAAATTGA AACAAGAGGA GAAGTACATG 
    TGGGCTGTTG TAGATGGTGT TAAAGAAAAG GTCGGAAATT TCAGAGTAGA ACCGCCTGGC 
    TTGTTCAGGG GACGTGGAGA ACATCCAAAG ATGGGGAAAC TTAAGCGCCG CATTCTACCA 
    AGTGATATTA CAATAAATAT TGGAAAAGGA GCTCCAGTCC CTGAGTGCCC GATACCTGGA 
    GAAAGTTGGA AAGAAGTCAA ACATGACAAT ACTGTTACAT GGTTGGCCTT TTGGAACGAT 
    CCAATTAACC CAAAGGATTT CAAGTATGTT TTCCTGGCAG CAAGCAGCTC ACTGAAGGGG 
    CAAAGTGACA AAGAGAAGTA TGAGAAGTCC AGAAAATTGA AGGGTCATAT ACAAAGAATT 
    CGTGAGAATT ATACAAAAGA TTTCAAAAAC AAAGATGTGA CGAGGAGGCA AATAGCTGTG 
    GCAACATACC TTATCGATAA ACTAGCCCTT AGGGCTGGCA ATGAAAAGGA CGACGATGAA 
    GCTGATACTG TTGGTTGCTG TACGCTGAAG GTTGATAATG TTACATGTAT GCCTCCAAAT 
    CAGATTCAGT TTGACTTTCT GGGTAAAGAT TCAATAAGAT ACTTTAACAC CGTAGAGGTT 
    GAAGAGCTTG TGTACAAGGC AATTGAGGGT TTCCGTGCTG GAAAGAAGCC AGGACAAGAT 
    CTATTTGACA AGATTGATAC ATCTAGGCTA AATGCTCACT TGAAGGATCT AATGCCTGGG 
    CTCACTGCAA AAGTGTTCCG TACATATAAT GCTTCCATAA CATTGGACGG AATCTTGCAT 
    GAAGAAACAG AAGATGGAAC TGTTTTAGAA AAAATCGCTG TCTATCAGCG GGCAAACAAA 
    GAGGTTGCCA TCATCTGTAA CCATCAGCGT AGTGTCTCGA AATCACATGA GTCCCAGATG 
    ACTAGAATGA ACGAAAAGAT TGATGAGTTG AAGTCCCAGA TGGAGGAGCT GAAAGCAGAC 
    TTGAACAAAG CAAAGAAAGG GAAGCCTCTA GGCTATGATT CAGAGGGGAA GCCAAAGAGG 
    AACTTGGCTC CTGAAGCGAT TCAAAAGAAG GTTGCAACAC TTGAAGGCAA GATAGAGAAG 
    ATGGAGATGG ATAAGAGGAT AAAAGAGGAT CTGAAGACAG TTGCGCTTGG AACGTCAAAG 
    ATCAACTATC TTGATCCTAG AATAACCGTG GCATGGTGCA AACGCCATGA AGTCCCTATT 
    GAGAAGATTT TCAACAAATC TCTCCTCGCG AAATTTGTAT GGGCAATGGA CGTTGACCCG 
    GAATTCAGAT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020406846.1
    CDS239..2953
    Translation

    Target ORF information:

    RefSeq Version XM_020551257.2
    Organism Zea mays
    Definition Zea mays topoisomerase I (Top1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_020551257.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTTG TCAACCCAAC CAACCTTGCT ATTGACGACG ATGACGACGA TACTCCCATT 
    TCATTCAAGA GGACGAGTAA CTCTACGAAG ACTGCCAGGC CCACCCCTTC CAAGCATGAG 
    GGGTCGTCAG GAGGTAATGG TGCACCTGTT AGGATCCCCA AACCTGTGTC TCCCAATCCG 
    CAGAAGAACG GAGCCACCGG GGCTTCAAGG CCGTTGCATA TGAAGCCACC GTCATCCAGT 
    CCAAACCATC GTCCTTCTGG ATCTGGCCAG CCAAACAGTT TGGCACAACA TAGTTCAAAG 
    GCTAATAGCA TTGACATGAG TAAACTGAAA AGACCTCTCG TCAAAGATGA GAGATCTGAT 
    GACTCAGATG ACGAGGTGCC GATTGGGTTA AGGAGAAAGG TTGAGGAGAA GAAGTTGAAG 
    GGAGTTGATA CTCGGGTTCA GAAGACAGAT GACTCAGATG ACGATGATAA GCCATTGGGT 
    CTCAAGATCA ACTCATCCAG AATGTCTTCC AACAGAGTGA ATAAGCCTGT TCTGCAGAGA 
    ACTGTACCAA AGGTTGAGCA GCCTGATGAA GATTCAGATG ATGATAAACC ATTGGCAAGC 
    AGGTTGCCCA GTAATGCCGG TCCAAAAAGT GGAGGTGATG TCTCTGAGGA TTCAGAGGAT 
    GAGAAGCCAC TGGCTGCCAG ATTTTCAAAA GTTGCTGGTA GTGGAAACCC AAAAATAGCT 
    TCTTCCAGCA AAGGGTTAAC CAGTGATACA AATGGCCAAC AAAGTTTGGG TAAAAGGCCT 
    CTAGACAATA GTAATCAAAC AAGTTCAGCT CTTAAGAAGG CTAAACCTTC AAACGTTTCA 
    GCTTCTGCAA TCGTTAGAAG AGAAATCAAG ACAGATGACA ACACACCTCT TGCACAAAGG 
    CTGAAAATGG GAGAGTCTTC GAAAGGCAAG TCATCTGCAA AGAGTGTCTT TAAGAAGAGT 
    CCTGCTTCTG TGAAGAAGGA CATCAAGAAA ATGAAAGGGA AAGGGAAATT AAAGAAGAAC 
    ATGAAAAATT CTCAGTTCTC AAAGATAATG AAGGTCCCCC CTGGATCTGG TGGAGGAAAG 
    AAATGGACTA CCTTGGAGCA CAATGGTGTT ATTTTCCCTC CCCCATACAA GCCTCATGGT 
    GTCAAAATGC TTTACAATGG GAAACCTATC GATCTGACAC CAGAACAAGA GGAGGTTGCA 
    ACAATGTTTG CTGTTATGAA AGACACAGAC TATGCGGCCA AGCCAACATT CATTGATAAC 
    TTTTTCACTG ATTGGAGAAA AGTTCTTGGT AAAAATCATA TTATTAAGAA CTTCGAGCTT 
    TGTGATTTCA CACCAATATA TGAATGGCAC CTCAGAGAGA AGGAGAGGAA GAAGCAGATG 
    ACAACAGAGG AGAAAAAAAC ATTGCGGGAA GACAAATTGA AACAAGAGGA GAAGTACATG 
    TGGGCTGTTG TAGATGGTGT TAAAGAAAAG GTCGGAAATT TCAGAGTAGA ACCGCCTGGC 
    TTGTTCAGGG GACGTGGAGA ACATCCAAAG ATGGGGAAAC TTAAGCGCCG CATTCTACCA 
    AGTGATATTA CAATAAATAT TGGAAAAGGA GCTCCAGTCC CTGAGTGCCC GATACCTGGA 
    GAAAGTTGGA AAGAAGTCAA ACATGACAAT ACTGTTACAT GGTTGGCCTT TTGGAACGAT 
    CCAATTAACC CAAAGGATTT CAAGTATGTT TTCCTGGCAG CAAGCAGCTC ACTGAAGGGG 
    CAAAGTGACA AAGAGAAGTA TGAGAAGTCC AGAAAATTGA AGGGTCATAT ACAAAGAATT 
    CGTGAGAATT ATACAAAAGA TTTCAAAAAC AAAGATGTGA CGAGGAGGCA AATAGCTGTG 
    GCAACATACC TTATCGATAA ACTAGCCCTT AGGGCTGGCA ATGAAAAGGA CGACGATGAA 
    GCTGATACTG TTGGTTGCTG TACGCTGAAG GTTGATAATG TTACATGTAT GCCTCCAAAT 
    CAGATTCAGT TTGACTTTCT GGGTAAAGAT TCAATAAGAT ACTTTAACAC CGTAGAGGTT 
    GAAGAGCTTG TGTACAAGGC AATTGAGGGT TTCCGTGCTG GAAAGAAGCC AGGACAAGAT 
    CTATTTGACA AGATTGATAC ATCTAGGCTA AATGCTCACT TGAAGGATCT AATGCCTGGG 
    CTCACTGCAA AAGTGTTCCG TACATATAAT GCTTCCATAA CATTGGACGG AATCTTGCAT 
    GAAGAAACAG AAGATGGAAC TGTTTTAGAA AAAATCGCTG TCTATCAGCG GGCAAACAAA 
    GAGGTTGCCA TCATCTGTAA CCATCAGCGT AGTGTCTCGA AATCACATGA GTCCCAGATG 
    ACTAGAATGA ACGAAAAGAT TGATGAGTTG AAGTCCCAGA TGGAGGAGCT GAAAGCAGAC 
    TTGAACAAAG CAAAGAAAGG GAAGCCTCTA GGCTATGATT CAGAGGGGAA GCCAAAGAGG 
    AACTTGGCTC CTGAAGCGAT TCAAAAGAAG GTTGCAACAC TTGAAGGCAA GATAGAGAAG 
    ATGGAGATGG ATAAGAGGAT AAAAGAGGAT CTGAAGACAG TTGCGCTTGG AACGTCAAAG 
    ATCAACTATC TTGATCCTAG AATAACCGTG GCATGGTGCA AACGCCATGA AGTCCCTATT 
    GAGAAGATTT TCAACAAATC TCTCCTCGCG AAATTTGTAT GGGCAATGGA CGTTGACCCG 
    GAATTCAGAT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100191474 ( XM_020551257.1 ) cDNA ORF clone, Zea mays -> XP_020406846.1 Zea mays topoisomerase I (Top1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OZa54077
    Clone ID Related Accession (Same CDS sequence) XM_020551257.2 , XM_020551257.1 , NM_001164334.2
    Accession Version XM_020551257.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1489852800000
    Organism Zea mays
    Product topoisomerase I isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024462.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTTG TCAACCCAAC CAACCTTGCT ATTGACGACG ATGACGACGA TACTCCCATT 
    TCATTCAAGA GGACGAGTAA CTCTACGAAG ACTGCCAGGC CCACCCCTTC CAAGCATGAG 
    GGGTCGTCAG GAGGTAATGG TGCACCTGTT AGGATCCCCA AACCTGTGTC TCCCAATCCG 
    CAGAAGAACG GAGCCACCGG GGCTTCAAGG CCGTTGCATA TGAAGCCACC GTCATCCAGT 
    CCAAACCATC GTCCTTCTGG ATCTGGCCAG CCAAACAGTT TGGCACAACA TAGTTCAAAG 
    GCTAATAGCA TTGACATGAG TAAACTGAAA AGACCTCTCG TCAAAGATGA GAGATCTGAT 
    GACTCAGATG ACGAGGTGCC GATTGGGTTA AGGAGAAAGG TTGAGGAGAA GAAGTTGAAG 
    GGAGTTGATA CTCGGGTTCA GAAGACAGAT GACTCAGATG ACGATGATAA GCCATTGGGT 
    CTCAAGATCA ACTCATCCAG AATGTCTTCC AACAGAGTGA ATAAGCCTGT TCTGCAGAGA 
    ACTGTACCAA AGGTTGAGCA GCCTGATGAA GATTCAGATG ATGATAAACC ATTGGCAAGC 
    AGGTTGCCCA GTAATGCCGG TCCAAAAAGT GGAGGTGATG TCTCTGAGGA TTCAGAGGAT 
    GAGAAGCCAC TGGCTGCCAG ATTTTCAAAA GTTGCTGGTA GTGGAAACCC AAAAATAGCT 
    TCTTCCAGCA AAGGGTTAAC CAGTGATACA AATGGCCAAC AAAGTTTGGG TAAAAGGCCT 
    CTAGACAATA GTAATCAAAC AAGTTCAGCT CTTAAGAAGG CTAAACCTTC AAACGTTTCA 
    GCTTCTGCAA TCGTTAGAAG AGAAATCAAG ACAGATGACA ACACACCTCT TGCACAAAGG 
    CTGAAAATGG GAGAGTCTTC GAAAGGCAAG TCATCTGCAA AGAGTGTCTT TAAGAAGAGT 
    CCTGCTTCTG TGAAGAAGGA CATCAAGAAA ATGAAAGGGA AAGGGAAATT AAAGAAGAAC 
    ATGAAAAATT CTCAGTTCTC AAAGATAATG AAGGTCCCCC CTGGATCTGG TGGAGGAAAG 
    AAATGGACTA CCTTGGAGCA CAATGGTGTT ATTTTCCCTC CCCCATACAA GCCTCATGGT 
    GTCAAAATGC TTTACAATGG GAAACCTATC GATCTGACAC CAGAACAAGA GGAGGTTGCA 
    ACAATGTTTG CTGTTATGAA AGACACAGAC TATGCGGCCA AGCCAACATT CATTGATAAC 
    TTTTTCACTG ATTGGAGAAA AGTTCTTGGT AAAAATCATA TTATTAAGAA CTTCGAGCTT 
    TGTGATTTCA CACCAATATA TGAATGGCAC CTCAGAGAGA AGGAGAGGAA GAAGCAGATG 
    ACAACAGAGG AGAAAAAAAC ATTGCGGGAA GACAAATTGA AACAAGAGGA GAAGTACATG 
    TGGGCTGTTG TAGATGGTGT TAAAGAAAAG GTCGGAAATT TCAGAGTAGA ACCGCCTGGC 
    TTGTTCAGGG GACGTGGAGA ACATCCAAAG ATGGGGAAAC TTAAGCGCCG CATTCTACCA 
    AGTGATATTA CAATAAATAT TGGAAAAGGA GCTCCAGTCC CTGAGTGCCC GATACCTGGA 
    GAAAGTTGGA AAGAAGTCAA ACATGACAAT ACTGTTACAT GGTTGGCCTT TTGGAACGAT 
    CCAATTAACC CAAAGGATTT CAAGTATGTT TTCCTGGCAG CAAGCAGCTC ACTGAAGGGG 
    CAAAGTGACA AAGAGAAGTA TGAGAAGTCC AGAAAATTGA AGGGTCATAT ACAAAGAATT 
    CGTGAGAATT ATACAAAAGA TTTCAAAAAC AAAGATGTGA CGAGGAGGCA AATAGCTGTG 
    GCAACATACC TTATCGATAA ACTAGCCCTT AGGGCTGGCA ATGAAAAGGA CGACGATGAA 
    GCTGATACTG TTGGTTGCTG TACGCTGAAG GTTGATAATG TTACATGTAT GCCTCCAAAT 
    CAGATTCAGT TTGACTTTCT GGGTAAAGAT TCAATAAGAT ACTTTAACAC CGTAGAGGTT 
    GAAGAGCTTG TGTACAAGGC AATTGAGGGT TTCCGTGCTG GAAAGAAGCC AGGACAAGAT 
    CTATTTGACA AGATTGATAC ATCTAGGCTA AATGCTCACT TGAAGGATCT AATGCCTGGG 
    CTCACTGCAA AAGTGTTCCG TACATATAAT GCTTCCATAA CATTGGACGG AATCTTGCAT 
    GAAGAAACAG AAGATGGAAC TGTTTTAGAA AAAATCGCTG TCTATCAGCG GGCAAACAAA 
    GAGGTTGCCA TCATCTGTAA CCATCAGCGT AGTGTCTCGA AATCACATGA GTCCCAGATG 
    ACTAGAATGA ACGAAAAGAT TGATGAGTTG AAGTCCCAGA TGGAGGAGCT GAAAGCAGAC 
    TTGAACAAAG CAAAGAAAGG GAAGCCTCTA GGCTATGATT CAGAGGGGAA GCCAAAGAGG 
    AACTTGGCTC CTGAAGCGAT TCAAAAGAAG GTTGCAACAC TTGAAGGCAA GATAGAGAAG 
    ATGGAGATGG ATAAGAGGAT AAAAGAGGAT CTGAAGACAG TTGCGCTTGG AACGTCAAAG 
    ATCAACTATC TTGATCCTAG AATAACCGTG GCATGGTGCA AACGCCATGA AGTCCCTATT 
    GAGAAGATTT TCAACAAATC TCTCCTCGCG AAATTTGTAT GGGCAATGGA CGTTGACCCG 
    GAATTCAGAT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020406846.1
    CDS240..2954
    Translation

    Target ORF information:

    RefSeq Version XM_020551257.1
    Organism Zea mays
    Definition Zea mays topoisomerase I (Top1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_020551257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGTTG TCAACCCAAC CAACCTTGCT ATTGACGACG ATGACGACGA TACTCCCATT 
    TCATTCAAGA GGACGAGTAA CTCTACGAAG ACTGCCAGGC CCACCCCTTC CAAGCATGAG 
    GGGTCGTCAG GAGGTAATGG TGCACCTGTT AGGATCCCCA AACCTGTGTC TCCCAATCCG 
    CAGAAGAACG GAGCCACCGG GGCTTCAAGG CCGTTGCATA TGAAGCCACC GTCATCCAGT 
    CCAAACCATC GTCCTTCTGG ATCTGGCCAG CCAAACAGTT TGGCACAACA TAGTTCAAAG 
    GCTAATAGCA TTGACATGAG TAAACTGAAA AGACCTCTCG TCAAAGATGA GAGATCTGAT 
    GACTCAGATG ACGAGGTGCC GATTGGGTTA AGGAGAAAGG TTGAGGAGAA GAAGTTGAAG 
    GGAGTTGATA CTCGGGTTCA GAAGACAGAT GACTCAGATG ACGATGATAA GCCATTGGGT 
    CTCAAGATCA ACTCATCCAG AATGTCTTCC AACAGAGTGA ATAAGCCTGT TCTGCAGAGA 
    ACTGTACCAA AGGTTGAGCA GCCTGATGAA GATTCAGATG ATGATAAACC ATTGGCAAGC 
    AGGTTGCCCA GTAATGCCGG TCCAAAAAGT GGAGGTGATG TCTCTGAGGA TTCAGAGGAT 
    GAGAAGCCAC TGGCTGCCAG ATTTTCAAAA GTTGCTGGTA GTGGAAACCC AAAAATAGCT 
    TCTTCCAGCA AAGGGTTAAC CAGTGATACA AATGGCCAAC AAAGTTTGGG TAAAAGGCCT 
    CTAGACAATA GTAATCAAAC AAGTTCAGCT CTTAAGAAGG CTAAACCTTC AAACGTTTCA 
    GCTTCTGCAA TCGTTAGAAG AGAAATCAAG ACAGATGACA ACACACCTCT TGCACAAAGG 
    CTGAAAATGG GAGAGTCTTC GAAAGGCAAG TCATCTGCAA AGAGTGTCTT TAAGAAGAGT 
    CCTGCTTCTG TGAAGAAGGA CATCAAGAAA ATGAAAGGGA AAGGGAAATT AAAGAAGAAC 
    ATGAAAAATT CTCAGTTCTC AAAGATAATG AAGGTCCCCC CTGGATCTGG TGGAGGAAAG 
    AAATGGACTA CCTTGGAGCA CAATGGTGTT ATTTTCCCTC CCCCATACAA GCCTCATGGT 
    GTCAAAATGC TTTACAATGG GAAACCTATC GATCTGACAC CAGAACAAGA GGAGGTTGCA 
    ACAATGTTTG CTGTTATGAA AGACACAGAC TATGCGGCCA AGCCAACATT CATTGATAAC 
    TTTTTCACTG ATTGGAGAAA AGTTCTTGGT AAAAATCATA TTATTAAGAA CTTCGAGCTT 
    TGTGATTTCA CACCAATATA TGAATGGCAC CTCAGAGAGA AGGAGAGGAA GAAGCAGATG 
    ACAACAGAGG AGAAAAAAAC ATTGCGGGAA GACAAATTGA AACAAGAGGA GAAGTACATG 
    TGGGCTGTTG TAGATGGTGT TAAAGAAAAG GTCGGAAATT TCAGAGTAGA ACCGCCTGGC 
    TTGTTCAGGG GACGTGGAGA ACATCCAAAG ATGGGGAAAC TTAAGCGCCG CATTCTACCA 
    AGTGATATTA CAATAAATAT TGGAAAAGGA GCTCCAGTCC CTGAGTGCCC GATACCTGGA 
    GAAAGTTGGA AAGAAGTCAA ACATGACAAT ACTGTTACAT GGTTGGCCTT TTGGAACGAT 
    CCAATTAACC CAAAGGATTT CAAGTATGTT TTCCTGGCAG CAAGCAGCTC ACTGAAGGGG 
    CAAAGTGACA AAGAGAAGTA TGAGAAGTCC AGAAAATTGA AGGGTCATAT ACAAAGAATT 
    CGTGAGAATT ATACAAAAGA TTTCAAAAAC AAAGATGTGA CGAGGAGGCA AATAGCTGTG 
    GCAACATACC TTATCGATAA ACTAGCCCTT AGGGCTGGCA ATGAAAAGGA CGACGATGAA 
    GCTGATACTG TTGGTTGCTG TACGCTGAAG GTTGATAATG TTACATGTAT GCCTCCAAAT 
    CAGATTCAGT TTGACTTTCT GGGTAAAGAT TCAATAAGAT ACTTTAACAC CGTAGAGGTT 
    GAAGAGCTTG TGTACAAGGC AATTGAGGGT TTCCGTGCTG GAAAGAAGCC AGGACAAGAT 
    CTATTTGACA AGATTGATAC ATCTAGGCTA AATGCTCACT TGAAGGATCT AATGCCTGGG 
    CTCACTGCAA AAGTGTTCCG TACATATAAT GCTTCCATAA CATTGGACGG AATCTTGCAT 
    GAAGAAACAG AAGATGGAAC TGTTTTAGAA AAAATCGCTG TCTATCAGCG GGCAAACAAA 
    GAGGTTGCCA TCATCTGTAA CCATCAGCGT AGTGTCTCGA AATCACATGA GTCCCAGATG 
    ACTAGAATGA ACGAAAAGAT TGATGAGTTG AAGTCCCAGA TGGAGGAGCT GAAAGCAGAC 
    TTGAACAAAG CAAAGAAAGG GAAGCCTCTA GGCTATGATT CAGAGGGGAA GCCAAAGAGG 
    AACTTGGCTC CTGAAGCGAT TCAAAAGAAG GTTGCAACAC TTGAAGGCAA GATAGAGAAG 
    ATGGAGATGG ATAAGAGGAT AAAAGAGGAT CTGAAGACAG TTGCGCTTGG AACGTCAAAG 
    ATCAACTATC TTGATCCTAG AATAACCGTG GCATGGTGCA AACGCCATGA AGTCCCTATT 
    GAGAAGATTT TCAACAAATC TCTCCTCGCG AAATTTGTAT GGGCAATGGA CGTTGACCCG 
    GAATTCAGAT TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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