LINJ_25_1540 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_25_1540
Entrez Gene ID	5069620
Full Name	calpain family cysteine protease-like protein
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001466148.1	XP_001466185.1	calpain family cysteine protease-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_25_1540 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_25_1540 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc03250	XM_001466148.1 Latest version!	Leishmania infantum JPCM5 calpain family cysteine protease-like protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_25_1540 ( XM_001466148.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001466185.1 Leishmania infantum JPCM5 calpain family cysteine protease-like protein partial mRNA.

Price & Availability↑

CloneID	OLc03250
Clone ID Related Accession (Same CDS sequence)	XM_001466148.1
Accession Version	XM_001466148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	calpain family cysteine protease-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009409). COMPLETENESS: incomplete on both ends.

ATGGGCAACG GTTGCTGCGG CTGCTGTGAC GCGAAGCCGG AGTACAAGCT CGGCAGGCCT 
CTTGTCTCGG GTAAAACCAC CCCGTGCTTC AATGATGGCC GCCTCTTCAA GATCGTCAAG 
GGCGATATCT GGTACTTCTA CAACGACACG CAGGACTATC AGATGAAGGT GACAGCCGAC 
TTCGGCAATG GCTCGAACAT CGTCGCGATG AGCGACACAG AACTCGTGTG CAAGAATGAC 
TCCGGCGCCT GCACCGCGAC CGTGTCCGTG TTCCCGCTCG AGACCAAGGC CATGGTGAAA 
ACCAAGGAGG TGAACGGCTT CAACGTAAAG TACTCCGGCA TCGCCTTCAC CGATGCGTAC 
CGACAGAAGC TGCGTCAGAA GGCGGCAGCG CAGGTCAGCG CCGACCTCGC CGAGATGCGC 
AAGCTGCGCG AGAGAAACCC GAAGGTCAAC AACCAAAATC GACTCCTGAA GGTGGCACGG 
ACAAAGGGCA AGATGTACGT TGACACGTCC TTTCCGCCCA CCTCTCAGTC GCTGATCCGC 
CCTGGCATCG ACCCAGACCC GGATGCGTGC CTGAAGCGGC CGGAGACGAT CGCATGGCGT 
CGCCCCGAAG ACTTCCTGCC GAAGAAGTGG CACCCGAAAA TTCGTCTCTT CGGCAACATC 
TCGCCCAAGG ACATTAGCCA GGGTCAGCTC GGGGATTGCT ACTACCTCTG CGCACTTGCG 
GCGCTGGCCG AGCACGACGC CGCCATCAAG GGCATCTTTA AGAACCGCCA CGGCTGCTGC 
ATCCGCAGCC AGGAGCGAAA GCATGGTGCG TGGCGCGTGA ACCTGAACAT AAGCGGGTGG 
TGGCGCACCA TCATCATCGA CTCGTATTTG CCCAGCGTGC AGCTGTTGCC GGTGTTCGCG 
CGCAACCGCA ACCACCCGAA CGAGCTGTGG GTGTCCTTTG CAGAGAAGGC CTACGCGAAG 
GTCTTCGGTT CGTACCAGGC GATCGTGGCT GGATACCCGT GGCAAGCCCT CGAGGACCTC 
ACCGGCTTCC CGGCCTACGA CTTTGGCAAC ATGTGGAGGA CAGCGCAGAC GGACACCGAT 
ACACGGAAGA AGCTCTTCGA CTCCCTCCAC TGGTGGAACG AGAAGAAGTA CCTGATCTGC 
ATTGCCACCC CGTCGAACGG CGCCCTAAAG ATGGCTGGTA AGCAGCGCTC AGCAAACCAG 
TTGGAGGCGC TCTTTGAGAA GGCCGGCCTG AACATCGGCC ACGCGTACTC GGTGCTGGAC 
GTGAAACACT TCCCACTGCA CCGGCTGTGC ATGCTGAAGA TCCGAAACCC GTGGGGGTCG 
CATGTGGAGT GGACCGGGGA CTGGGGAGAT GACAGTCCGC TGTGGAACCG CTACCCATTC 
ATCAAGCTCG CCTGCCGTCC TCAGAAGAAG GCGGATGGCA TCTTCTGGAT GGAGTGGCGC 
GATGTGTCTA AGTTCTTCGA CAGCGGCTCC GTGTGCTTCC GCCGTGGTCA CTGGTTCCGT 
TCGTGGTATG ACTACCGCGT CATCGGCAGC TTTGAGGACC TTGTGTGCGA CACCGCGCTG 
CTGATCATCG TGAACAAGAC GTCGCAGTTT CCGGCGTACA TTTCGCTGCA CCAGAAGGAC 
TGCCGCGGGC TGCCGACCAG CGACCCGGAC AGCAAGTACG CTTGCGTGAT GATCAGCATT 
TCCGAGGGCG ACATCAACGG CTCGCAGCAG AAGGTGGTAG CAAACTCCAG CGAGAACCCG 
GAGGAGCCGA GCACCGAATA CCTCTTCCAG GAGTCTCGCT CTGTCTCCCT CTACTATAAG 
TTCACAAAGG CCCACAAGTA CCTCGTCATT CCGCGTCGCA TGAAAAGCAG CACCGGCAAC 
AACGTCCCAA AGAAGAAGTA CGTCATCGCG CTCCGCACGC AGACCAAGGT GTCCAGCGAG 
GACGTCGTCG TGAACATTGT GCGCCTTGAC AAGAACAACG CGGTGTTCAA GAACGTGGCC 
TCTTTCGACG CTGGCACACC GACGAGCCTC AACACGCTGT ACCAGATCAA GGACGGCAAC 
AACGTCTTTC GCACCTACCG CAGCGACAAC TTGCGCAAGG GCAAGAAGCA GCACAACGCG 
GAGTTCGAGC TGGTGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001466185.1
CDS	1..2121
Translation	MGNGCCGCCDAKPEYKLGRPLVSGKTTPCFNDGRLFKIVKGDIWYFYNDTQDYQMKVTADFGNGSNIVAMSDTELVCKNDSGACTATVSVFPLETKAMVKTKEVNGFNVKYSGIAFTDAYRQKLRQKAAAQVSADLAEMRKLRERNPKVNNQNRLLKVARTKGKMYVDTSFPPTSQSLIRPGIDPDPDACLKRPETIAWRRPEDFLPKKWHPKIRLFGNISPKDISQGQLGDCYYLCALAALAEHDAAIKGIFKNRHGCCIRSQERKHGAWRVNLNISGWWRTIIIDSYLPSVQLLPVFARNRNHPNELWVSFAEKAYAKVFGSYQAIVAGYPWQALEDLTGFPAYDFGNMWRTAQTDTDTRKKLFDSLHWWNEKKYLICIATPSNGALKMAGKQRSANQLEALFEKAGLNIGHAYSVLDVKHFPLHRLCMLKIRNPWGSHVEWTGDWGDDSPLWNRYPFIKLACRPQKKADGIFWMEWRDVSKFFDSGSVCFRRGHWFRSWYDYRVIGSFEDLVCDTALLIIVNKTSQFPAYISLHQKDCRGLPTSDPDSKYACVMISISEGDINGSQQKVVANSSENPEEPSTEYLFQESRSVSLYYKFTKAHKYLVIPRRMKSSTGNNVPKKKYVIALRTQTKVSSEDVVVNIVRLDKNNAVFKNVASFDAGTPTSLNTLYQIKDGNNVFRTYRSDNLRKGKKQHNAEFELVM

Target ORF information:

RefSeq Version	XM_001466148.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 calpain family cysteine protease-like protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001466148.1

ORF Insert Sequence:

ATGGGCAACG GTTGCTGCGG CTGCTGTGAC GCGAAGCCGG AGTACAAGCT CGGCAGGCCT 
CTTGTCTCGG GTAAAACCAC CCCGTGCTTC AATGATGGCC GCCTCTTCAA GATCGTCAAG 
GGCGATATCT GGTACTTCTA CAACGACACG CAGGACTATC AGATGAAGGT GACAGCCGAC 
TTCGGCAATG GCTCGAACAT CGTCGCGATG AGCGACACAG AACTCGTGTG CAAGAATGAC 
TCCGGCGCCT GCACCGCGAC CGTGTCCGTG TTCCCGCTCG AGACCAAGGC CATGGTGAAA 
ACCAAGGAGG TGAACGGCTT CAACGTAAAG TACTCCGGCA TCGCCTTCAC CGATGCGTAC 
CGACAGAAGC TGCGTCAGAA GGCGGCAGCG CAGGTCAGCG CCGACCTCGC CGAGATGCGC 
AAGCTGCGCG AGAGAAACCC GAAGGTCAAC AACCAAAATC GACTCCTGAA GGTGGCACGG 
ACAAAGGGCA AGATGTACGT TGACACGTCC TTTCCGCCCA CCTCTCAGTC GCTGATCCGC 
CCTGGCATCG ACCCAGACCC GGATGCGTGC CTGAAGCGGC CGGAGACGAT CGCATGGCGT 
CGCCCCGAAG ACTTCCTGCC GAAGAAGTGG CACCCGAAAA TTCGTCTCTT CGGCAACATC 
TCGCCCAAGG ACATTAGCCA GGGTCAGCTC GGGGATTGCT ACTACCTCTG CGCACTTGCG 
GCGCTGGCCG AGCACGACGC CGCCATCAAG GGCATCTTTA AGAACCGCCA CGGCTGCTGC 
ATCCGCAGCC AGGAGCGAAA GCATGGTGCG TGGCGCGTGA ACCTGAACAT AAGCGGGTGG 
TGGCGCACCA TCATCATCGA CTCGTATTTG CCCAGCGTGC AGCTGTTGCC GGTGTTCGCG 
CGCAACCGCA ACCACCCGAA CGAGCTGTGG GTGTCCTTTG CAGAGAAGGC CTACGCGAAG 
GTCTTCGGTT CGTACCAGGC GATCGTGGCT GGATACCCGT GGCAAGCCCT CGAGGACCTC 
ACCGGCTTCC CGGCCTACGA CTTTGGCAAC ATGTGGAGGA CAGCGCAGAC GGACACCGAT 
ACACGGAAGA AGCTCTTCGA CTCCCTCCAC TGGTGGAACG AGAAGAAGTA CCTGATCTGC 
ATTGCCACCC CGTCGAACGG CGCCCTAAAG ATGGCTGGTA AGCAGCGCTC AGCAAACCAG 
TTGGAGGCGC TCTTTGAGAA GGCCGGCCTG AACATCGGCC ACGCGTACTC GGTGCTGGAC 
GTGAAACACT TCCCACTGCA CCGGCTGTGC ATGCTGAAGA TCCGAAACCC GTGGGGGTCG 
CATGTGGAGT GGACCGGGGA CTGGGGAGAT GACAGTCCGC TGTGGAACCG CTACCCATTC 
ATCAAGCTCG CCTGCCGTCC TCAGAAGAAG GCGGATGGCA TCTTCTGGAT GGAGTGGCGC 
GATGTGTCTA AGTTCTTCGA CAGCGGCTCC GTGTGCTTCC GCCGTGGTCA CTGGTTCCGT 
TCGTGGTATG ACTACCGCGT CATCGGCAGC TTTGAGGACC TTGTGTGCGA CACCGCGCTG 
CTGATCATCG TGAACAAGAC GTCGCAGTTT CCGGCGTACA TTTCGCTGCA CCAGAAGGAC 
TGCCGCGGGC TGCCGACCAG CGACCCGGAC AGCAAGTACG CTTGCGTGAT GATCAGCATT 
TCCGAGGGCG ACATCAACGG CTCGCAGCAG AAGGTGGTAG CAAACTCCAG CGAGAACCCG 
GAGGAGCCGA GCACCGAATA CCTCTTCCAG GAGTCTCGCT CTGTCTCCCT CTACTATAAG 
TTCACAAAGG CCCACAAGTA CCTCGTCATT CCGCGTCGCA TGAAAAGCAG CACCGGCAAC 
AACGTCCCAA AGAAGAAGTA CGTCATCGCG CTCCGCACGC AGACCAAGGT GTCCAGCGAG 
GACGTCGTCG TGAACATTGT GCGCCTTGAC AAGAACAACG CGGTGTTCAA GAACGTGGCC 
TCTTTCGACG CTGGCACACC GACGAGCCTC AACACGCTGT ACCAGATCAA GGACGGCAAC 
AACGTCTTTC GCACCTACCG CAGCGACAAC TTGCGCAAGG GCAAGAAGCA GCACAACGCG 
GAGTTCGAGC TGGTGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_25_1540 cDNA ORF clone, Leishmania infantum JPCM5

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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