LINJ_20_1240 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_20_1240
Entrez Gene ID	5068670
Full Name	putative calpain-like cysteine peptidase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001465217.1	XP_001465254.1	putative calpain-like cysteine peptidase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_20_1240 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_20_1240 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc02391	XM_001465217.1 Latest version!	Leishmania infantum JPCM5 putative calpain-like cysteine peptidase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_20_1240 ( XM_001465217.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001465254.1 Leishmania infantum JPCM5 putative calpain-like cysteine peptidase partial mRNA.

Price & Availability↑

CloneID	OLc02391
Clone ID Related Accession (Same CDS sequence)	XM_001465217.1
Accession Version	XM_001465217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative calpain-like cysteine peptidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009404). COMPLETENESS: incomplete on both ends.

ATGTTCGATG AGTTTCGTGA GGACTCGGTA GAGCCGCTAC CAATGGATGC CAGTAAGAGC 
TCATCAACGC CATGGGTGAA CTGGGGGCCG TCCATCGACG GCGAGGCTAC GCCATGCTTC 
AAAGGCGGTT TTCTCTATCG GATTATCGAT TCCAAGAAGA AAGTCTGGGC TCTGTATAAC 
GACACGTTCG TCTACGAAAT GCATGCCACC TTTATTTTCG GGCCTGTGTC GAATATGGAG 
CCCATCACCG CCTCGCTCTC TGCGTCGGAG AGCGGCGGCG AGGTGGATGA CAAGAACCGC 
GTCGTCGCCA GCGTTGAGGG CAACGAGCTA ACGATCTCCA TGGTTGTTTA TCCGATGGAG 
ACGATCGAGT TCGCCCGGGG CGACAAGTCG AAGTACAGAT GCCACTTCGA CGGCAAGCCG 
CTCTCAAAAG AGTACCTTCA ACGCGAAACA GCCTTGGCAA ACGCCGAGCT AGAAAGGAAC 
AAGGTGACGC TCGCGAGGTT CATCCACACC TCCACAGATC CTGAGGATGT GCTGGCGTTG 
TGCAAAAAAC ACCGTTTGAC GTTTATCGAC CCTTCCTTCC CTCCTGGCCA GCACTCGCTT 
GACGGGGATC GAGGCCTGAT TGCGCCGAGC GGGTGGAGGC GCCCAGAGGC GTATCTGCCT 
CTCGCGTTGA AGGGTCAAAT TCGTCTGTTT CGGCATCGTG TGACCCCCTC GGCCACACAG 
CGTGGCGAAT TGGGCAATTC GTGGGTGGTG AGCGCCATGG CGGCGCTGGC GGAACACCCG 
GACTACATCA AGGATATGTT TCGGCACCCC ATCAGCTCTG AAGAGACGCA GCGAGACGAG 
GCGGTTGGGG CATACCGGGC TTGGCTGAAC AAAGACGGTC TCTGGAGCTC GGTACTCGTC 
GACAGCTATC TGCCCGTGGT GGGCAAGCAG CAGCGCTACG CCCGCAGCGC CAACGGGCCG 
TGCGAGATGT GGGTGTCGTA TCTCGAGAAG GTGTATGCGA AGCGTTATCA CAGCTACATC 
AACATCGCCG GCGGCGATCC GCTCTTTGCA ATTCGCGACT TTACCGGATT TCCCACGGCG 
CGGCTGGACG TCCGCTTTCG CGAGTGTGTT ACGGACCCGG TGAAGAGCGA TATGTTTTTC 
CTTCGCATGG TACGCGACTA CGAGAGCGGG CACCTTGTGC TACTGAGCAC TCCCGGCAGC 
GGTGACCCGC GCTCTGCCCA TAGCACATAC AAGGAGAAGG GCATTTTTGT GGGCTACGCC 
TATGCAGTGC TAGAAGCTCG CGCGATTGAA TTGCCGACGC TGCGGCGGAA GAAGGTGCCA 
CCGCTGCGGC TGCTGCGGCT GCGCAACGCG TGGGAGGCGG CGACCAAGTG GAGTGGCAGG 
TGGTCTGACG ACTCGCCGAT GTGGCAGGCG CACCCGGAAG CGTGTGTTGC GTTTCCGCAG 
CACAGCGGGA ATGATGGAAC TTTCATCATG GAGTGGTCGG AGGTGCTGGA GATCTTTGTC 
GGCTGCGGTG TTGTGTTCAA CCACTTCGGC TACGCGGACT ACCGCATCCC CTTCGAGTTC 
CGGGGCTCTG TGCCGGACCT GTGTCTCGAG ATTCACGTGA CGGAGCCGAC AACGTTGACG 
CTCATTCTGT CGCAGCCAGA CAGCAAAGGC ACGGTGAGGG AGTCGGAGGA CTACAACCCT 
GTCATGATCT CCATAGCGGG CGCCTCCTCC GCCACGACGA GCCCGGTTTC CCAGAATGGC 
AGCAAGCGTA GTAGCGGGAC CTCCAGCCAC TCCATGATGA CGGCCAGTAC ACAGCTGGTG 
GCGCGCGATT ACTCTCTCCT CGTCAATTCC TCTGCGGACG CCGAGACGCC GTCGAAGAGC 
TTCACGTTCT TGCAGGGCCG CGACATAAGC GCCATATACA CGTTCGTCCC AGAGCAGTCG 
CCGTACCTCG TCGTGCCACG TCTGCTTGTG CAGCAGTCTA GCGGCAGCAA CGGCACGAGG 
GCGTCCAGTG CAGCTGCGAC CTCGGCATCA AACAGCGCTG TGACGTGTCC ATGCGTGCTC 
GGCGTTCTTT CGCAGCTTGC CTTTACCCCA AACGGACAGG CACACGTTCG TCTGCGCAAG 
TTGCCAGCGA ACAGTCTCGT CTTTGACAAC TACCTCAGCT TCGCCAACGA CTCCGTGGAG 
GTCGAGAAGA CCTTCTACGT GAAACGCTCC GCCACCGGTG TGGCGGAGCG CAGCGCCTCA 
GAACTACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001465254.1
CDS	1..2232
Translation	MFDEFREDSVEPLPMDASKSSSTPWVNWGPSIDGEATPCFKGGFLYRIIDSKKKVWALYNDTFVYEMHATFIFGPVSNMEPITASLSASESGGEVDDKNRVVASVEGNELTISMVVYPMETIEFARGDKSKYRCHFDGKPLSKEYLQRETALANAELERNKVTLARFIHTSTDPEDVLALCKKHRLTFIDPSFPPGQHSLDGDRGLIAPSGWRRPEAYLPLALKGQIRLFRHRVTPSATQRGELGNSWVVSAMAALAEHPDYIKDMFRHPISSEETQRDEAVGAYRAWLNKDGLWSSVLVDSYLPVVGKQQRYARSANGPCEMWVSYLEKVYAKRYHSYINIAGGDPLFAIRDFTGFPTARLDVRFRECVTDPVKSDMFFLRMVRDYESGHLVLLSTPGSGDPRSAHSTYKEKGIFVGYAYAVLEARAIELPTLRRKKVPPLRLLRLRNAWEAATKWSGRWSDDSPMWQAHPEACVAFPQHSGNDGTFIMEWSEVLEIFVGCGVVFNHFGYADYRIPFEFRGSVPDLCLEIHVTEPTTLTLILSQPDSKGTVRESEDYNPVMISIAGASSATTSPVSQNGSKRSSGTSSHSMMTASTQLVARDYSLLVNSSADAETPSKSFTFLQGRDISAIYTFVPEQSPYLVVPRLLVQQSSGSNGTRASSAAATSASNSAVTCPCVLGVLSQLAFTPNGQAHVRLRKLPANSLVFDNYLSFANDSVEVEKTFYVKRSATGVAERSASELQ

Target ORF information:

RefSeq Version	XM_001465217.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative calpain-like cysteine peptidase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001465217.1

ORF Insert Sequence:

ATGTTCGATG AGTTTCGTGA GGACTCGGTA GAGCCGCTAC CAATGGATGC CAGTAAGAGC 
TCATCAACGC CATGGGTGAA CTGGGGGCCG TCCATCGACG GCGAGGCTAC GCCATGCTTC 
AAAGGCGGTT TTCTCTATCG GATTATCGAT TCCAAGAAGA AAGTCTGGGC TCTGTATAAC 
GACACGTTCG TCTACGAAAT GCATGCCACC TTTATTTTCG GGCCTGTGTC GAATATGGAG 
CCCATCACCG CCTCGCTCTC TGCGTCGGAG AGCGGCGGCG AGGTGGATGA CAAGAACCGC 
GTCGTCGCCA GCGTTGAGGG CAACGAGCTA ACGATCTCCA TGGTTGTTTA TCCGATGGAG 
ACGATCGAGT TCGCCCGGGG CGACAAGTCG AAGTACAGAT GCCACTTCGA CGGCAAGCCG 
CTCTCAAAAG AGTACCTTCA ACGCGAAACA GCCTTGGCAA ACGCCGAGCT AGAAAGGAAC 
AAGGTGACGC TCGCGAGGTT CATCCACACC TCCACAGATC CTGAGGATGT GCTGGCGTTG 
TGCAAAAAAC ACCGTTTGAC GTTTATCGAC CCTTCCTTCC CTCCTGGCCA GCACTCGCTT 
GACGGGGATC GAGGCCTGAT TGCGCCGAGC GGGTGGAGGC GCCCAGAGGC GTATCTGCCT 
CTCGCGTTGA AGGGTCAAAT TCGTCTGTTT CGGCATCGTG TGACCCCCTC GGCCACACAG 
CGTGGCGAAT TGGGCAATTC GTGGGTGGTG AGCGCCATGG CGGCGCTGGC GGAACACCCG 
GACTACATCA AGGATATGTT TCGGCACCCC ATCAGCTCTG AAGAGACGCA GCGAGACGAG 
GCGGTTGGGG CATACCGGGC TTGGCTGAAC AAAGACGGTC TCTGGAGCTC GGTACTCGTC 
GACAGCTATC TGCCCGTGGT GGGCAAGCAG CAGCGCTACG CCCGCAGCGC CAACGGGCCG 
TGCGAGATGT GGGTGTCGTA TCTCGAGAAG GTGTATGCGA AGCGTTATCA CAGCTACATC 
AACATCGCCG GCGGCGATCC GCTCTTTGCA ATTCGCGACT TTACCGGATT TCCCACGGCG 
CGGCTGGACG TCCGCTTTCG CGAGTGTGTT ACGGACCCGG TGAAGAGCGA TATGTTTTTC 
CTTCGCATGG TACGCGACTA CGAGAGCGGG CACCTTGTGC TACTGAGCAC TCCCGGCAGC 
GGTGACCCGC GCTCTGCCCA TAGCACATAC AAGGAGAAGG GCATTTTTGT GGGCTACGCC 
TATGCAGTGC TAGAAGCTCG CGCGATTGAA TTGCCGACGC TGCGGCGGAA GAAGGTGCCA 
CCGCTGCGGC TGCTGCGGCT GCGCAACGCG TGGGAGGCGG CGACCAAGTG GAGTGGCAGG 
TGGTCTGACG ACTCGCCGAT GTGGCAGGCG CACCCGGAAG CGTGTGTTGC GTTTCCGCAG 
CACAGCGGGA ATGATGGAAC TTTCATCATG GAGTGGTCGG AGGTGCTGGA GATCTTTGTC 
GGCTGCGGTG TTGTGTTCAA CCACTTCGGC TACGCGGACT ACCGCATCCC CTTCGAGTTC 
CGGGGCTCTG TGCCGGACCT GTGTCTCGAG ATTCACGTGA CGGAGCCGAC AACGTTGACG 
CTCATTCTGT CGCAGCCAGA CAGCAAAGGC ACGGTGAGGG AGTCGGAGGA CTACAACCCT 
GTCATGATCT CCATAGCGGG CGCCTCCTCC GCCACGACGA GCCCGGTTTC CCAGAATGGC 
AGCAAGCGTA GTAGCGGGAC CTCCAGCCAC TCCATGATGA CGGCCAGTAC ACAGCTGGTG 
GCGCGCGATT ACTCTCTCCT CGTCAATTCC TCTGCGGACG CCGAGACGCC GTCGAAGAGC 
TTCACGTTCT TGCAGGGCCG CGACATAAGC GCCATATACA CGTTCGTCCC AGAGCAGTCG 
CCGTACCTCG TCGTGCCACG TCTGCTTGTG CAGCAGTCTA GCGGCAGCAA CGGCACGAGG 
GCGTCCAGTG CAGCTGCGAC CTCGGCATCA AACAGCGCTG TGACGTGTCC ATGCGTGCTC 
GGCGTTCTTT CGCAGCTTGC CTTTACCCCA AACGGACAGG CACACGTTCG TCTGCGCAAG 
TTGCCAGCGA ACAGTCTCGT CTTTGACAAC TACCTCAGCT TCGCCAACGA CTCCGTGGAG 
GTCGAGAAGA CCTTCTACGT GAAACGCTCC GCCACCGGTG TGGCGGAGCG CAGCGCCTCA 
GAACTACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_20_1240 cDNA ORF clone, Leishmania infantum JPCM5

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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