LINJ_20_1230 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_20_1230
Entrez Gene ID	5068669
Full Name	putative calpain-like cysteine peptidase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001465216.1	XP_001465253.1	putative calpain-like cysteine peptidase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_20_1230 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_20_1230 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc02390	XM_001465216.1 Latest version!	Leishmania infantum JPCM5 putative calpain-like cysteine peptidase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_20_1230 ( XM_001465216.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001465253.1 Leishmania infantum JPCM5 putative calpain-like cysteine peptidase partial mRNA.

Price & Availability↑

CloneID	OLc02390
Clone ID Related Accession (Same CDS sequence)	XM_001465216.1
Accession Version	XM_001465216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative calpain-like cysteine peptidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009404). COMPLETENESS: incomplete on both ends.

ATGACGGACA TGAACGGCAA GGTAGACGAG TTCGATGAGG GCTCCGTCGT ACAAAAGAAG 
TACGGCCCCA ACGACTACCG GTACGGCGAG CCGGTGTACA AGGGCGATGC AACGCCGTGC 
TTCGAAGACG GCCTTCTCTT CCGCATTGTG GAGAAAAGCA ACAACCGCTG GTCCTTTTAC 
AATGACACCA TCCACACGCA GATGAATGTG CAGATCGTGT TTGGCCGCAA TTCCTCCCTG 
CGTGCGCTGG AGAACACAGA AATGGTGAAG CTGGAGAACG GATCCTACCG CGCTACTGTG 
ACGGTGTACC CGATGGAGAC GGAGCTCTTT GTGGAGGGCG AGGTAAACGG CTTCACTAGC 
AACATTCGTG CTCTGCCGCT GACGGAGGAC TACCTCAAGG ACATGGCTCG TCAGGATTAC 
GAGTTCATCA AACAGGAAAC GGCGACCTTG TACAACGCTG TGGACAAGAA CGCTACGACT 
GATGACATGG TTCGCAAGTG CGTGCAGAGC AAAATCAAGT TTGTCGACTT TGCCTTCCCG 
CCGGAGCAGA AGTCGCTGCA GATCGGCTCT CTCATGAAGC TGAAGGTGCT GGCGTGGGAG 
CGCCCGGGCA TGTTCCTTTC CGACGAGAAC GCCCGGCAGG CGCGCCTGTT TCGCAACGGC 
GTCCACCCAT CCAACATCGA CGAGGGTGAT TTGGGTGACT CGTGGCTGAC GGGCGCGATG 
GCAGCGTTGT CTGAGTTCCC GGACAAGATC CGTGATATCT TCCGCCACCC CGAGAGCGTG 
GAGCAGGGGC AGAAGGAGCG CGAGTGCGGC ATCTACCGTG TGACGCTGAA CAAGAACGGC 
TGGTGGACGA GCTTGATTGT CGACGACTAC CTGCCGGTGG CCGGCGGCCG CCCAAAGTTT 
GCCCGCTCCA AGGGCGATCC GGCCGAGCTG TGGCCCTCCA TTCTCCAGAA GGCGTACGCG 
AAGGTCTTTG GCGGGTACGG CTTCATTGTC GCTGGAGACC CGCTGCACGC GCTGCAGGAC 
ATGTCCGGAT TCCCGTGCTC CTCTTTTGAT AACGCGTTCG TGGAGAGCAC TATCAACGAG 
ACGTATGAGC TGTTCGCGCA TCTCAAGAAC TACAGTGACC TGGGCTACCA GCTTGTGTTC 
ACCACCCCGA CCCGCGAAGC CATCATGACG GCTCGCGGCG CCATCAGCTG CGGCTCCCCC 
GTGCAGGCGG AGCGTGCGTA CGAGAGGTCC AGCCTGCTGC TGGGGCACAC CTACTCCGTG 
ATTCGCGTGG GATACTTCGC GGAGCACGAC CTGCGCCTTC TGCAGCTGCG GAACCCGTGG 
TCGCAGGCCT CCTCGGACTG GAATGGGCCT TGGAACAAGC GCGACGAGAA CTGGAACAAG 
TACACGGAGG TGGCAGACTA CTTCCACTTT GACAGCAGCG ATGACGGCAC CTTCTTCATG 
GAGTGGACCG ACGTGCAGGA CTACTTTGTC AGCTGCGGCG TGTGCTTCAT TCAGAGCCCC 
ACGTACGACT ACCGCATCCG CGGCACCTTC TTCCAAAACG TGCCGACGAC ATGCCTGGAG 
ATCTCCGTTA CACGTCCGAT GCTGATCAAC ATTATTCTCT CGCAGGAAGA CAAGCGTGGC 
ACCGACAAGA GCGAGAACGC GCCGATCATG ATTAGTGTGG CGCACGGCAT GGGGGCGATG 
ACGCCGATGC GCGTGGACTT GAACAGCGGC TTCGACAACG ACCACCCGTC GCCCGAGTAT 
GCATTCCTGC AGAGTCGTGA CGTGAGCATG TTCTACGAAT TCAAGCCGGA GAACTCGCCG 
TACCTCGTGG TGCCGCGTGC CATGAGCCAG TTCGCCAAGC TTCCCTTCAC ACTAGGCCTA 
CTGTGCCCGT ACGAGATCGG CAAGGAGACG AGCGAGGTCC GCGTGGCCTT CCGCGCCCTC 
GCCCCGGAGA ACCGCATCTT CGACAACTTC CAGAAGTTCA ACTGCGAGAC CGTGGCCACA 
GAGACGGAGT TCCAGGCAAA AGGCCCGAAG CAGTTTTTCC CTGACATCTA CGTGGGTACC 
ATCATCCAGA CGTCTGACGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001465253.1
CDS	1..2064
Translation	MTDMNGKVDEFDEGSVVQKKYGPNDYRYGEPVYKGDATPCFEDGLLFRIVEKSNNRWSFYNDTIHTQMNVQIVFGRNSSLRALENTEMVKLENGSYRATVTVYPMETELFVEGEVNGFTSNIRALPLTEDYLKDMARQDYEFIKQETATLYNAVDKNATTDDMVRKCVQSKIKFVDFAFPPEQKSLQIGSLMKLKVLAWERPGMFLSDENARQARLFRNGVHPSNIDEGDLGDSWLTGAMAALSEFPDKIRDIFRHPESVEQGQKERECGIYRVTLNKNGWWTSLIVDDYLPVAGGRPKFARSKGDPAELWPSILQKAYAKVFGGYGFIVAGDPLHALQDMSGFPCSSFDNAFVESTINETYELFAHLKNYSDLGYQLVFTTPTREAIMTARGAISCGSPVQAERAYERSSLLLGHTYSVIRVGYFAEHDLRLLQLRNPWSQASSDWNGPWNKRDENWNKYTEVADYFHFDSSDDGTFFMEWTDVQDYFVSCGVCFIQSPTYDYRIRGTFFQNVPTTCLEISVTRPMLINIILSQEDKRGTDKSENAPIMISVAHGMGAMTPMRVDLNSGFDNDHPSPEYAFLQSRDVSMFYEFKPENSPYLVVPRAMSQFAKLPFTLGLLCPYEIGKETSEVRVAFRALAPENRIFDNFQKFNCETVATETEFQAKGPKQFFPDIYVGTIIQTSDD

Target ORF information:

RefSeq Version	XM_001465216.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative calpain-like cysteine peptidase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001465216.1

ORF Insert Sequence:

ATGACGGACA TGAACGGCAA GGTAGACGAG TTCGATGAGG GCTCCGTCGT ACAAAAGAAG 
TACGGCCCCA ACGACTACCG GTACGGCGAG CCGGTGTACA AGGGCGATGC AACGCCGTGC 
TTCGAAGACG GCCTTCTCTT CCGCATTGTG GAGAAAAGCA ACAACCGCTG GTCCTTTTAC 
AATGACACCA TCCACACGCA GATGAATGTG CAGATCGTGT TTGGCCGCAA TTCCTCCCTG 
CGTGCGCTGG AGAACACAGA AATGGTGAAG CTGGAGAACG GATCCTACCG CGCTACTGTG 
ACGGTGTACC CGATGGAGAC GGAGCTCTTT GTGGAGGGCG AGGTAAACGG CTTCACTAGC 
AACATTCGTG CTCTGCCGCT GACGGAGGAC TACCTCAAGG ACATGGCTCG TCAGGATTAC 
GAGTTCATCA AACAGGAAAC GGCGACCTTG TACAACGCTG TGGACAAGAA CGCTACGACT 
GATGACATGG TTCGCAAGTG CGTGCAGAGC AAAATCAAGT TTGTCGACTT TGCCTTCCCG 
CCGGAGCAGA AGTCGCTGCA GATCGGCTCT CTCATGAAGC TGAAGGTGCT GGCGTGGGAG 
CGCCCGGGCA TGTTCCTTTC CGACGAGAAC GCCCGGCAGG CGCGCCTGTT TCGCAACGGC 
GTCCACCCAT CCAACATCGA CGAGGGTGAT TTGGGTGACT CGTGGCTGAC GGGCGCGATG 
GCAGCGTTGT CTGAGTTCCC GGACAAGATC CGTGATATCT TCCGCCACCC CGAGAGCGTG 
GAGCAGGGGC AGAAGGAGCG CGAGTGCGGC ATCTACCGTG TGACGCTGAA CAAGAACGGC 
TGGTGGACGA GCTTGATTGT CGACGACTAC CTGCCGGTGG CCGGCGGCCG CCCAAAGTTT 
GCCCGCTCCA AGGGCGATCC GGCCGAGCTG TGGCCCTCCA TTCTCCAGAA GGCGTACGCG 
AAGGTCTTTG GCGGGTACGG CTTCATTGTC GCTGGAGACC CGCTGCACGC GCTGCAGGAC 
ATGTCCGGAT TCCCGTGCTC CTCTTTTGAT AACGCGTTCG TGGAGAGCAC TATCAACGAG 
ACGTATGAGC TGTTCGCGCA TCTCAAGAAC TACAGTGACC TGGGCTACCA GCTTGTGTTC 
ACCACCCCGA CCCGCGAAGC CATCATGACG GCTCGCGGCG CCATCAGCTG CGGCTCCCCC 
GTGCAGGCGG AGCGTGCGTA CGAGAGGTCC AGCCTGCTGC TGGGGCACAC CTACTCCGTG 
ATTCGCGTGG GATACTTCGC GGAGCACGAC CTGCGCCTTC TGCAGCTGCG GAACCCGTGG 
TCGCAGGCCT CCTCGGACTG GAATGGGCCT TGGAACAAGC GCGACGAGAA CTGGAACAAG 
TACACGGAGG TGGCAGACTA CTTCCACTTT GACAGCAGCG ATGACGGCAC CTTCTTCATG 
GAGTGGACCG ACGTGCAGGA CTACTTTGTC AGCTGCGGCG TGTGCTTCAT TCAGAGCCCC 
ACGTACGACT ACCGCATCCG CGGCACCTTC TTCCAAAACG TGCCGACGAC ATGCCTGGAG 
ATCTCCGTTA CACGTCCGAT GCTGATCAAC ATTATTCTCT CGCAGGAAGA CAAGCGTGGC 
ACCGACAAGA GCGAGAACGC GCCGATCATG ATTAGTGTGG CGCACGGCAT GGGGGCGATG 
ACGCCGATGC GCGTGGACTT GAACAGCGGC TTCGACAACG ACCACCCGTC GCCCGAGTAT 
GCATTCCTGC AGAGTCGTGA CGTGAGCATG TTCTACGAAT TCAAGCCGGA GAACTCGCCG 
TACCTCGTGG TGCCGCGTGC CATGAGCCAG TTCGCCAAGC TTCCCTTCAC ACTAGGCCTA 
CTGTGCCCGT ACGAGATCGG CAAGGAGACG AGCGAGGTCC GCGTGGCCTT CCGCGCCCTC 
GCCCCGGAGA ACCGCATCTT CGACAACTTC CAGAAGTTCA ACTGCGAGAC CGTGGCCACA 
GAGACGGAGT TCCAGGCAAA AGGCCCGAAG CAGTTTTTCC CTGACATCTA CGTGGGTACC 
ATCATCCAGA CGTCTGACGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_20_1230 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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