LINJ_05_0530 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_05_0530
Entrez Gene ID	10966095
Full Name	kinetoplast-associated protein-like protein
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_003392168.1	XP_003392216.1	kinetoplast-associated protein-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_05_0530 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_05_0530 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc07456	XM_003392168.1 Latest version!	Leishmania infantum JPCM5 kinetoplast-associated protein-like protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_05_0530 ( XM_003392168.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_003392216.1 Leishmania infantum JPCM5 kinetoplast-associated protein-like protein partial mRNA.

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CloneID	OLc07456
Clone ID Related Accession (Same CDS sequence)	XM_003392168.1
Accession Version	XM_003392168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	kinetoplast-associated protein-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009390). COMPLETENESS: incomplete on both ends.

ATGCAACCGA GATTGGTGCT GCCCAGCTCG CAGCCTCTAA GGATCTCAGC GCAGTATCCA 
GTGGGGTCGT CTTCGGCAGA ACCGTCATCA TCATCGGCCG CGGCTCGGCC TCGAGGGTCG 
TATGGCGTGT CCATCGACCC TGCACAAGCC CCGAATGTCG CCGCCGTGGC GGTAGCGACG 
CCACTGCCGC CACCATCTTC CTCGTCACGA CTTCTGCAGG AGATCGAGGA AACCCGTAAA 
CTCCTTCGCA CCTTCCGCTC GTTGAAGGAG CGCGGCGCGA GCCGTGCTGA GCTGGCAGAT 
CTTCACCTCC GTGTCAAGGC CCTCAAGGCG GCGCAGCAGT GCCAGCAACC AAATTCCGGC 
GCATGCAAGG CGACAGCTCT CCCTCTGGCT ACGTCCACTA CCGCGGACGC AGAAGCTGCT 
GCAGCAACAG CAGCGGCGCG TCCGTCGCTC GCTTGCTCAC CCAAGGCGGC GCCACCATTG 
CCATGCGTGC ACCGTCACGC AGACGTCGCA TTCCACGAGG GAGCAGCTGG GTGGACTGCG 
GAATCAAGCC ACAGTTTGCA TCAACCTCAG CAGCGAGGCG TCGAGGAGGC GCACGTTGCA 
CATCGCGACG GACGGCTACC GGAAGGCAGG CATCATCATC ATCATCACCG TCACCGTCAC 
CGCCACAATG ACGTCCGTGT GGACCGGGCG GCATCGCTGT CCATCTCTAC GAGCCTATCT 
ACAGCCGCAG CACAGGCTTC GACGGCAGTG TGCAACGGCA TCTTTAACGC CGCCAGCAGC 
GGCAGCGCTG GCGTTTGCAG TGCCGTGGCG CTGCAGCCAA GCACGGCGTC GGCAGTGCCC 
GGATACCTTG TGCAGATCAG CTCGATCGTC TTTGCCATCG CGCTCGCGGA GGCGCACACG 
CGTCGTGAGA TCGCGCAACG ATGGGAGCGC CGGTGGCTGC GGCACTGGGC GAACTTCAAG 
GAGGAGCGCA TCGCCATAGC CCTGCGCCCG CCCACCACAG CGACAGCGGC GCTGTCACGT 
CCGCAGCTGC AGCCGGAGAG GTGGGCGGAG GCTGCCCCGA TGGCGCTACC TCACGAATGG 
CAGCAGCGGC AACAGGCGGT GTCGCCGGCG ACGGCGAGTG TGGAGGGGAG CAGTGTTGCG 
ACAGCCACCG CAGCCCGCAC TGTCGGCGCT TCTCTTGCGC CAGTGCCGGC CAAGAGCGTT 
GGGGAGGCGT TCACAGCTAC AACAAGCAGC CCAGCGGTGG CGGGTCTTGA AGAGACGTCG 
CCGCTCTCGG CCGGTCACCG CAGTGGCGAC GAGGCGCACC ATGTCGCTGA AGAACAAGAG 
AAGACGATCC CCGACGTGGA CGACGCCGCT GTGGCAAAAG ACTTGCTGGC GTGGGCAAGT 
CCTCTTCCAC AGCACCAGCA GCATCCGGCA GCACCGGTAA CAGAACAAGA GTTAGAGGCG 
AGCGCATGCG CCGCTGCTGA TGCGACTGCC GAAGCGGCTG CAGCCGTGAG GAACGTGGAG 
GACGAAGAAG CTACCGCGCG CACAGCGACG GAGAGCGCTG CGGCAGAGGC ACGGAAGCGT 
ATGAAGATGG CTGAAGTCAC AGCGCGCCTC AGAGCTGAGG AGCCGCAGGA GTCTGTGCAG 
TCATTCATGG CCGCTGAAGC AGGGGCCCGT GTCACCCTCG AAGGCGATGA GGCCCGCTTG 
AGGATCTCGC ACGTTGCAGC GGAGAGGGAC GGCGCCCAGC GCGTTGCCCT TGCGGCAGCA 
GAGGCGGAGT CTCGGCGGAG AGCGCAGGAG GAACAGGTGG GTCAGGAGGC AGCCGCGCGA 
GCAGCGGCGT CCAAGAGAGA GGAAGAGAAG GAGCGAGAAG CTGCTGCGCG TCGTGCGGCC 
GAGGAGCAAC AGCAAGCTGC AGCAGACACC GAAGCTGCAG AGCGGCGTTC CGTGGAGCAG 
CAGTGGCAGG AAGCCGTCCG TCTGTCCGAG TCACTTCTGT CCGATCGGAT CAAGGATGCT 
GTTGCAGCGG CTCCGCCGTT GGCTTACTCG AAGGGCACGC GTGCACCCGA ACGTCCATCG 
CGCGATGAGC TAGCAATGAT GGAGGACGCA CAAGCAAGAG AGGCCGCGGA CGCGCAGGCC 
TGCCTACGTG AGCGGCAGCA AGCAGCGGAG CAGGGTGTTG CGGCTTTCGG CGCAGCAGCG 
GATGCTGTGC TTGGTGAGTG GATCAGCACG GCAGCGATGG AGGCCGAGCA GGCAGCGTCG 
GTTGAGGCAG CCAGGCGGAG AAAGGCAGAA GAAGCCGCAG CTCAAGCGAA GGCTGTGGCG 
CAGGAGAGGG CATCTGCGGC TGAGGCTGCA CGCGCCGCGG TCGCACGGAC CGAAGACGAA 
GGCAGGGCTG CACTGACAGC TTCGGAAGAG GAGGTGTGGC AGTGGCTGCA GCTGCAGCAC 
GGAGCTGGCC TTGAGTCGAC GCGTCTCGCA GCTCTTGCAG CCGAGGGAAA GGCCGCGTGC 
CACCGTGCTG AGGAGGAGGC AGCTGCTGCC CGTCTGACGT TGGAGATGAG TGCATCCAAA 
GAAGCAGCGC GTGCCAAGGC CATCCAGGAG GCCGTGCGCT TGCAGGCGGA GCAGCAGGCA 
GCTGCGATGA CCGTTACGCT GTGCTCCGCC GTCGCCGAGT TCTCCAAGAA TCTTGTGGAC 
GGCTGCCTAG CCGAGGCAGC ACAGCAGGTA TCCGAACAAG TAAAAGCGGC CGCTCTGGAG 
GAGTTGCAGA TGCAACGGCG CGAGAGGGTC GAAGGGGAAG AGGCGTCGGC GCGAGATGAC 
ACGTACGGCG ACGCGCTTGA CGAACTGGCG AGACTGCGCG AGGAGGAAGC AGCCGCACAG 
GTCCGCAAGC GCATGGAGGT CGAAGCTGTG GCTGTCGCGA AGGAGCAGCA GCAGCAGCAG 
CAGCGTATGC AAGCGGCTGC ACAGTATTTT CGGATTTGCG AGTTAAGTGA AGCTCTGCTG 
CTCGAATTCC TGCACGACGC TGCCGCCACC GCCGCGGCGG CACGCACGGC AGCCGTCGCT 
AACGATCGTC TTGCCGAAGC GTCACCGCCG GCTGCCTTAC CAGAAGGGGA AGAGGGAGCA 
GCGAGCGCTG TCATGGAGGA CGGACAGCAG CACCAGCACC ACCACCCGTC TGCCGATGAC 
GCCGCTGAAG AGCCGAACGC TCGCGCAGAC AAGAGGCAGG GGGACGGCGT CGACAGCCCT 
GACAAGGCTA ACGTAGACAC AGCAGCACCG GGAGCCGTGG CCGCTACAGC GTCACTCTCT 
CTCTCGCCGT CGTCTTCTGC CGGCCCGTCG TCTTTCTTCG TGGAATCCGT CTCTGCGGAT 
GCGACTCCGA GGGCTGTCAC AACTGGCGTG CTGCAAGGCC ATGCGGTCGA GGGCTTAGGG 
CTTCTGTCGC CCACCGTTTT AGTGCACGTC GTCGATGGCC TCCTCTCTGA TGTTCTCCGT 
GATGCAGCCA TGGAGGCGCA GAGGGGCGGT GCTGGTGCTG TCGGCGCGAT CAGCGAGGAT 
ATAGCAAGCC GAGCACACGC AGGGCAGAGG GCGAGGGAGC ACTGCAAGCC CCTGCCCAAC 
GGCTCCGACG CGTCCTTCGT GTCGATTGGC CATGGCAGCT CGCACTCAAC CCTTGGGTGC 
TCCCCACCGT GGGAGCCGCA GCCGAAGCCT CTGGAGCTAC CGCTCGCCCT CGTGGTCCGT 
GATGCTGCAG GCGACCGTGT GCCACAAGTG CAGTACACGG GGGGCGAGCC CAAGGACTGT 
GCGGAGGCTG GCGTGGTTAT TGGCGGGGCC GGCAACGTCA CGGCGTCTCC GGCTGAGGTC 
TCGGACGTGG ACAGCTGCGC CCCACACCGG CGCATCGTCT CACCTCTCTT GATACCATCA 
ATGGACGTCG CCATCGTCAG CACCTCCTCC ACTTCGACAG CTTCAGTAAC GGACGGAAGA 
AGAAGAGGCT GCGCTCGCGG CTCCGCCGGC GCGACTGCAA CGGCGTCGTC ACCGCTGCAC 
TGCTTCTCCA CGGCATCCTC GGCCGACGAC GACGACGCGG CCTGCAGCTC CCTCACCGCC 
CCGGGGGCGC CATGCCCGCT CAATCTGCTG CAGCACGGTG TTTTGTCTCC GCTACTGTGC 
CAGCAGCACG GCGGCAACGG CGGCGTACCC CACGATGGCG GCGAGGCAAG CACGCCTACC 
GACAGTGAAG ATGCGGCGGG CACTACAACG GTCGCTGCAT TGGCTGATCG GCCCTCGTTA 
TCGGAGAATA GCCGAGCAAG ACGGCAGCTT CCAGAGAGCA CCGGGACCGG CGACCACGAG 
GCCGCCTCCG ACGCAGATGG AATGCTGTCC GCGGTGGTCG CAATGGACCA CAGAGTCATG 
GGAATCACAG AGGACTTGCG CGCTTTTCAG CTAGACGCAC CGCCACCGCC ACATGGCGCA 
GCCGAGCAGC GCGCTGGAGT GCGTATCGCT GCCTATGATA CACTCACGGC AGACGCTGTG 
GCGGCGGTGG CAGACTCTCT GGTGCAGCGC GCGGTTCACG ACGCGTCTCT TCTCTTCGAC 
TTTCGGGGGC AGTCGGCAGT GCCGAACACC GACAGCGATC ACCAGGAGTC TCACGACGCC 
GTCAAGGTCG ACCACGCAGC GGCACCACCG TTTCTTTTCG CCATCGCTCA GAGCCACGAG 
CAGGGCACGG CGGAAGGCAA GAGCAGTGAC AATGGCAGCG GTGGTGGGAC GTCCTCATCC 
GCTTGCGCCG GGGATGTCGA TGCACATACC GAGAAGAGGG CCAGCATCGC GGAGATGGGC 
TCAGGCTTCA CGAGTGGCGA CGCTCGGGAA GCCACCCTGC GTGTTAATCG GTCGCTTTCG 
CCGGCCGCTG CCGCGGTGGC CGTGGAAGGC ATCGCTGGTT CGCATGCGCC GCTCGCAAGC 
CTGTCTACCC TCGTCTCCGC CGTGGCGGCA CCAAACGACT TCGTGGCTCG GCTGCGGCAG 
CAAGAGGCAG AGAAGAAGGC GGCGGCGCAT CAACAAAACC GCTACCTCAC CAGTCGCGAG 
CTGGCGCTCG TCGCGGCGCG TTACTTGACG TCGGAAGCGG CCGCAGCGCA TGTGATGGAG 
GTTGGCCCCA CCACCACGCT TTCGGCCCGT GCCGTGGAGC TTGTTCTGTC GGTGGGGCTC 
GTGCGTGCAG CCACACTGGA GAATCGACGC GCGCGCCGCC GCATGGAGAA GGCGGAGAAG 
GGAGACGGGA GCAGCGCCAG GGGCGATGGC AGCACATCGG GTGGCGGTGT GCTCGTGGGA 
GAGAGCGGGT CGCAGAAGTT GACCCCAGGC GGTGGAGCTG CCTCAGCGAT GATGGACAGC 
CCGGACGCGC CGCTCCCGTC GCTGGGCGGC GGTGGTGATG GAGGTGGATT TCCGACGGGT 
ACCATCGGCG GGGCAGGCGC GGCAGGGGAC GACGAGGGCG TCAATGTTCA ACTCGTGCCA 
CCAACCGCAC GTTGCCGCAG CGGCGGCGGC GACTTGAGCA TGCCTGAGCC TACCGCCTCG 
AGCGCTGTGG GCGATTTTGC GCACGCGAGC GGGGACGTCA AGGATACGGG CATGAGTGGC 
GGGCCCACAG ATCTGGGGTC CCAGCCCCTG TCACCGCGGT CGTCGTCATC GATCGGGTCC 
GCCTCTCAGC CGACGACAGG TGGTGGCGTC AAAGGCGCGC TCGCAGCGAA TCCAGCGCAG 
CACCAGGTTG GGGTGCGCGA CAAGCTGCCA GCGGACTGGG CCACCGCGCT GCGCGGCGTG 
GCGGAGCGGG TCGCGCGCGA CTTCATCGAC TACGCCTCCC GCCGCGTGCT GCTCGGCCAA 
GGTGAGGGCC AGTCGAACCA AGATCACCTG CGCACTGCGC GACGCATCCA CATCGACGCC 
CTCGAGGGCG TCGATGTGCC GGCGCTCTTC ACGCAAGAGC TGCAGCCGCG CGATGTGGCG 
CGGCTGACCT CCTACTACGT CGAGCTAGCG ACCAACGGGC CGCGTGACCG CGTCGACCCT 
CTCTGCGCCC CCGCCGCGCT TGGCGAGCAC AACATCTTCG GTCACCGCGG CAGCGTGAGA 
AACGAGGATG TCGCCAGCTT CGGCAACCTC GACGCAGCGT CGGCGAGCGT GTTTGCGCCA 
CCGACGCGGC CCCGGTCCCT CTTCGCATCC TCCCACCTCC TTCCATCGCA CAAAAACCAC 
CTGCTGTCGG CAGCCACAGC CATTCACGTG CGTCGCGCCG GACTGCTGCA TCTGGTGCTG 
GAGCAGTGCC TGTCGAACGT GCTGCATGAC CTGGTCGGGG ACACGGTGGG CTGGCTCTGG 
ACCGCCTGTC TGCAAGCAGC ACCGCCGGGA GCCGCACCGA ATGGCACGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003392216.1
CDS	1..6294
Translation	MQPRLVLPSSQPLRISAQYPVGSSSAEPSSSSAAARPRGSYGVSIDPAQAPNVAAVAVATPLPPPSSSSRLLQEIEETRKLLRTFRSLKERGASRAELADLHLRVKALKAAQQCQQPNSGACKATALPLATSTTADAEAAAATAAARPSLACSPKAAPPLPCVHRHADVAFHEGAAGWTAESSHSLHQPQQRGVEEAHVAHRDGRLPEGRHHHHHHRHRHRHNDVRVDRAASLSISTSLSTAAAQASTAVCNGIFNAASSGSAGVCSAVALQPSTASAVPGYLVQISSIVFAIALAEAHTRREIAQRWERRWLRHWANFKEERIAIALRPPTTATAALSRPQLQPERWAEAAPMALPHEWQQRQQAVSPATASVEGSSVATATAARTVGASLAPVPAKSVGEAFTATTSSPAVAGLEETSPLSAGHRSGDEAHHVAEEQEKTIPDVDDAAVAKDLLAWASPLPQHQQHPAAPVTEQELEASACAAADATAEAAAAVRNVEDEEATARTATESAAAEARKRMKMAEVTARLRAEEPQESVQSFMAAEAGARVTLEGDEARLRISHVAAERDGAQRVALAAAEAESRRRAQEEQVGQEAAARAAASKREEEKEREAAARRAAEEQQQAAADTEAAERRSVEQQWQEAVRLSESLLSDRIKDAVAAAPPLAYSKGTRAPERPSRDELAMMEDAQAREAADAQACLRERQQAAEQGVAAFGAAADAVLGEWISTAAMEAEQAASVEAARRRKAEEAAAQAKAVAQERASAAEAARAAVARTEDEGRAALTASEEEVWQWLQLQHGAGLESTRLAALAAEGKAACHRAEEEAAAARLTLEMSASKEAARAKAIQEAVRLQAEQQAAAMTVTLCSAVAEFSKNLVDGCLAEAAQQVSEQVKAAALEELQMQRRERVEGEEASARDDTYGDALDELARLREEEAAAQVRKRMEVEAVAVAKEQQQQQQRMQAAAQYFRICELSEALLLEFLHDAAATAAAARTAAVANDRLAEASPPAALPEGEEGAASAVMEDGQQHQHHHPSADDAAEEPNARADKRQGDGVDSPDKANVDTAAPGAVAATASLSLSPSSSAGPSSFFVESVSADATPRAVTTGVLQGHAVEGLGLLSPTVLVHVVDGLLSDVLRDAAMEAQRGGAGAVGAISEDIASRAHAGQRAREHCKPLPNGSDASFVSIGHGSSHSTLGCSPPWEPQPKPLELPLALVVRDAAGDRVPQVQYTGGEPKDCAEAGVVIGGAGNVTASPAEVSDVDSCAPHRRIVSPLLIPSMDVAIVSTSSTSTASVTDGRRRGCARGSAGATATASSPLHCFSTASSADDDDAACSSLTAPGAPCPLNLLQHGVLSPLLCQQHGGNGGVPHDGGEASTPTDSEDAAGTTTVAALADRPSLSENSRARRQLPESTGTGDHEAASDADGMLSAVVAMDHRVMGITEDLRAFQLDAPPPPHGAAEQRAGVRIAAYDTLTADAVAAVADSLVQRAVHDASLLFDFRGQSAVPNTDSDHQESHDAVKVDHAAAPPFLFAIAQSHEQGTAEGKSSDNGSGGGTSSSACAGDVDAHTEKRASIAEMGSGFTSGDAREATLRVNRSLSPAAAAVAVEGIAGSHAPLASLSTLVSAVAAPNDFVARLRQQEAEKKAAAHQQNRYLTSRELALVAARYLTSEAAAAHVMEVGPTTTLSARAVELVLSVGLVRAATLENRRARRRMEKAEKGDGSSARGDGSTSGGGVLVGESGSQKLTPGGGAASAMMDSPDAPLPSLGGGGDGGGFPTGTIGGAGAAGDDEGVNVQLVPPTARCRSGGGDLSMPEPTASSAVGDFAHASGDVKDTGMSGGPTDLGSQPLSPRSSSSIGSASQPTTGGGVKGALAANPAQHQVGVRDKLPADWATALRGVAERVARDFIDYASRRVLLGQGEGQSNQDHLRTARRIHIDALEGVDVPALFTQELQPRDVARLTSYYVELATNGPRDRVDPLCAPAALGEHNIFGHRGSVRNEDVASFGNLDAASASVFAPPTRPRSLFASSHLLPSHKNHLLSAATAIHVRRAGLLHLVLEQCLSNVLHDLVGDTVGWLWTACLQAAPPGAAPNGTQ

Target ORF information:

RefSeq Version	XM_003392168.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 kinetoplast-associated protein-like protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003392168.1

ORF Insert Sequence:

ATGCAACCGA GATTGGTGCT GCCCAGCTCG CAGCCTCTAA GGATCTCAGC GCAGTATCCA 
GTGGGGTCGT CTTCGGCAGA ACCGTCATCA TCATCGGCCG CGGCTCGGCC TCGAGGGTCG 
TATGGCGTGT CCATCGACCC TGCACAAGCC CCGAATGTCG CCGCCGTGGC GGTAGCGACG 
CCACTGCCGC CACCATCTTC CTCGTCACGA CTTCTGCAGG AGATCGAGGA AACCCGTAAA 
CTCCTTCGCA CCTTCCGCTC GTTGAAGGAG CGCGGCGCGA GCCGTGCTGA GCTGGCAGAT 
CTTCACCTCC GTGTCAAGGC CCTCAAGGCG GCGCAGCAGT GCCAGCAACC AAATTCCGGC 
GCATGCAAGG CGACAGCTCT CCCTCTGGCT ACGTCCACTA CCGCGGACGC AGAAGCTGCT 
GCAGCAACAG CAGCGGCGCG TCCGTCGCTC GCTTGCTCAC CCAAGGCGGC GCCACCATTG 
CCATGCGTGC ACCGTCACGC AGACGTCGCA TTCCACGAGG GAGCAGCTGG GTGGACTGCG 
GAATCAAGCC ACAGTTTGCA TCAACCTCAG CAGCGAGGCG TCGAGGAGGC GCACGTTGCA 
CATCGCGACG GACGGCTACC GGAAGGCAGG CATCATCATC ATCATCACCG TCACCGTCAC 
CGCCACAATG ACGTCCGTGT GGACCGGGCG GCATCGCTGT CCATCTCTAC GAGCCTATCT 
ACAGCCGCAG CACAGGCTTC GACGGCAGTG TGCAACGGCA TCTTTAACGC CGCCAGCAGC 
GGCAGCGCTG GCGTTTGCAG TGCCGTGGCG CTGCAGCCAA GCACGGCGTC GGCAGTGCCC 
GGATACCTTG TGCAGATCAG CTCGATCGTC TTTGCCATCG CGCTCGCGGA GGCGCACACG 
CGTCGTGAGA TCGCGCAACG ATGGGAGCGC CGGTGGCTGC GGCACTGGGC GAACTTCAAG 
GAGGAGCGCA TCGCCATAGC CCTGCGCCCG CCCACCACAG CGACAGCGGC GCTGTCACGT 
CCGCAGCTGC AGCCGGAGAG GTGGGCGGAG GCTGCCCCGA TGGCGCTACC TCACGAATGG 
CAGCAGCGGC AACAGGCGGT GTCGCCGGCG ACGGCGAGTG TGGAGGGGAG CAGTGTTGCG 
ACAGCCACCG CAGCCCGCAC TGTCGGCGCT TCTCTTGCGC CAGTGCCGGC CAAGAGCGTT 
GGGGAGGCGT TCACAGCTAC AACAAGCAGC CCAGCGGTGG CGGGTCTTGA AGAGACGTCG 
CCGCTCTCGG CCGGTCACCG CAGTGGCGAC GAGGCGCACC ATGTCGCTGA AGAACAAGAG 
AAGACGATCC CCGACGTGGA CGACGCCGCT GTGGCAAAAG ACTTGCTGGC GTGGGCAAGT 
CCTCTTCCAC AGCACCAGCA GCATCCGGCA GCACCGGTAA CAGAACAAGA GTTAGAGGCG 
AGCGCATGCG CCGCTGCTGA TGCGACTGCC GAAGCGGCTG CAGCCGTGAG GAACGTGGAG 
GACGAAGAAG CTACCGCGCG CACAGCGACG GAGAGCGCTG CGGCAGAGGC ACGGAAGCGT 
ATGAAGATGG CTGAAGTCAC AGCGCGCCTC AGAGCTGAGG AGCCGCAGGA GTCTGTGCAG 
TCATTCATGG CCGCTGAAGC AGGGGCCCGT GTCACCCTCG AAGGCGATGA GGCCCGCTTG 
AGGATCTCGC ACGTTGCAGC GGAGAGGGAC GGCGCCCAGC GCGTTGCCCT TGCGGCAGCA 
GAGGCGGAGT CTCGGCGGAG AGCGCAGGAG GAACAGGTGG GTCAGGAGGC AGCCGCGCGA 
GCAGCGGCGT CCAAGAGAGA GGAAGAGAAG GAGCGAGAAG CTGCTGCGCG TCGTGCGGCC 
GAGGAGCAAC AGCAAGCTGC AGCAGACACC GAAGCTGCAG AGCGGCGTTC CGTGGAGCAG 
CAGTGGCAGG AAGCCGTCCG TCTGTCCGAG TCACTTCTGT CCGATCGGAT CAAGGATGCT 
GTTGCAGCGG CTCCGCCGTT GGCTTACTCG AAGGGCACGC GTGCACCCGA ACGTCCATCG 
CGCGATGAGC TAGCAATGAT GGAGGACGCA CAAGCAAGAG AGGCCGCGGA CGCGCAGGCC 
TGCCTACGTG AGCGGCAGCA AGCAGCGGAG CAGGGTGTTG CGGCTTTCGG CGCAGCAGCG 
GATGCTGTGC TTGGTGAGTG GATCAGCACG GCAGCGATGG AGGCCGAGCA GGCAGCGTCG 
GTTGAGGCAG CCAGGCGGAG AAAGGCAGAA GAAGCCGCAG CTCAAGCGAA GGCTGTGGCG 
CAGGAGAGGG CATCTGCGGC TGAGGCTGCA CGCGCCGCGG TCGCACGGAC CGAAGACGAA 
GGCAGGGCTG CACTGACAGC TTCGGAAGAG GAGGTGTGGC AGTGGCTGCA GCTGCAGCAC 
GGAGCTGGCC TTGAGTCGAC GCGTCTCGCA GCTCTTGCAG CCGAGGGAAA GGCCGCGTGC 
CACCGTGCTG AGGAGGAGGC AGCTGCTGCC CGTCTGACGT TGGAGATGAG TGCATCCAAA 
GAAGCAGCGC GTGCCAAGGC CATCCAGGAG GCCGTGCGCT TGCAGGCGGA GCAGCAGGCA 
GCTGCGATGA CCGTTACGCT GTGCTCCGCC GTCGCCGAGT TCTCCAAGAA TCTTGTGGAC 
GGCTGCCTAG CCGAGGCAGC ACAGCAGGTA TCCGAACAAG TAAAAGCGGC CGCTCTGGAG 
GAGTTGCAGA TGCAACGGCG CGAGAGGGTC GAAGGGGAAG AGGCGTCGGC GCGAGATGAC 
ACGTACGGCG ACGCGCTTGA CGAACTGGCG AGACTGCGCG AGGAGGAAGC AGCCGCACAG 
GTCCGCAAGC GCATGGAGGT CGAAGCTGTG GCTGTCGCGA AGGAGCAGCA GCAGCAGCAG 
CAGCGTATGC AAGCGGCTGC ACAGTATTTT CGGATTTGCG AGTTAAGTGA AGCTCTGCTG 
CTCGAATTCC TGCACGACGC TGCCGCCACC GCCGCGGCGG CACGCACGGC AGCCGTCGCT 
AACGATCGTC TTGCCGAAGC GTCACCGCCG GCTGCCTTAC CAGAAGGGGA AGAGGGAGCA 
GCGAGCGCTG TCATGGAGGA CGGACAGCAG CACCAGCACC ACCACCCGTC TGCCGATGAC 
GCCGCTGAAG AGCCGAACGC TCGCGCAGAC AAGAGGCAGG GGGACGGCGT CGACAGCCCT 
GACAAGGCTA ACGTAGACAC AGCAGCACCG GGAGCCGTGG CCGCTACAGC GTCACTCTCT 
CTCTCGCCGT CGTCTTCTGC CGGCCCGTCG TCTTTCTTCG TGGAATCCGT CTCTGCGGAT 
GCGACTCCGA GGGCTGTCAC AACTGGCGTG CTGCAAGGCC ATGCGGTCGA GGGCTTAGGG 
CTTCTGTCGC CCACCGTTTT AGTGCACGTC GTCGATGGCC TCCTCTCTGA TGTTCTCCGT 
GATGCAGCCA TGGAGGCGCA GAGGGGCGGT GCTGGTGCTG TCGGCGCGAT CAGCGAGGAT 
ATAGCAAGCC GAGCACACGC AGGGCAGAGG GCGAGGGAGC ACTGCAAGCC CCTGCCCAAC 
GGCTCCGACG CGTCCTTCGT GTCGATTGGC CATGGCAGCT CGCACTCAAC CCTTGGGTGC 
TCCCCACCGT GGGAGCCGCA GCCGAAGCCT CTGGAGCTAC CGCTCGCCCT CGTGGTCCGT 
GATGCTGCAG GCGACCGTGT GCCACAAGTG CAGTACACGG GGGGCGAGCC CAAGGACTGT 
GCGGAGGCTG GCGTGGTTAT TGGCGGGGCC GGCAACGTCA CGGCGTCTCC GGCTGAGGTC 
TCGGACGTGG ACAGCTGCGC CCCACACCGG CGCATCGTCT CACCTCTCTT GATACCATCA 
ATGGACGTCG CCATCGTCAG CACCTCCTCC ACTTCGACAG CTTCAGTAAC GGACGGAAGA 
AGAAGAGGCT GCGCTCGCGG CTCCGCCGGC GCGACTGCAA CGGCGTCGTC ACCGCTGCAC 
TGCTTCTCCA CGGCATCCTC GGCCGACGAC GACGACGCGG CCTGCAGCTC CCTCACCGCC 
CCGGGGGCGC CATGCCCGCT CAATCTGCTG CAGCACGGTG TTTTGTCTCC GCTACTGTGC 
CAGCAGCACG GCGGCAACGG CGGCGTACCC CACGATGGCG GCGAGGCAAG CACGCCTACC 
GACAGTGAAG ATGCGGCGGG CACTACAACG GTCGCTGCAT TGGCTGATCG GCCCTCGTTA 
TCGGAGAATA GCCGAGCAAG ACGGCAGCTT CCAGAGAGCA CCGGGACCGG CGACCACGAG 
GCCGCCTCCG ACGCAGATGG AATGCTGTCC GCGGTGGTCG CAATGGACCA CAGAGTCATG 
GGAATCACAG AGGACTTGCG CGCTTTTCAG CTAGACGCAC CGCCACCGCC ACATGGCGCA 
GCCGAGCAGC GCGCTGGAGT GCGTATCGCT GCCTATGATA CACTCACGGC AGACGCTGTG 
GCGGCGGTGG CAGACTCTCT GGTGCAGCGC GCGGTTCACG ACGCGTCTCT TCTCTTCGAC 
TTTCGGGGGC AGTCGGCAGT GCCGAACACC GACAGCGATC ACCAGGAGTC TCACGACGCC 
GTCAAGGTCG ACCACGCAGC GGCACCACCG TTTCTTTTCG CCATCGCTCA GAGCCACGAG 
CAGGGCACGG CGGAAGGCAA GAGCAGTGAC AATGGCAGCG GTGGTGGGAC GTCCTCATCC 
GCTTGCGCCG GGGATGTCGA TGCACATACC GAGAAGAGGG CCAGCATCGC GGAGATGGGC 
TCAGGCTTCA CGAGTGGCGA CGCTCGGGAA GCCACCCTGC GTGTTAATCG GTCGCTTTCG 
CCGGCCGCTG CCGCGGTGGC CGTGGAAGGC ATCGCTGGTT CGCATGCGCC GCTCGCAAGC 
CTGTCTACCC TCGTCTCCGC CGTGGCGGCA CCAAACGACT TCGTGGCTCG GCTGCGGCAG 
CAAGAGGCAG AGAAGAAGGC GGCGGCGCAT CAACAAAACC GCTACCTCAC CAGTCGCGAG 
CTGGCGCTCG TCGCGGCGCG TTACTTGACG TCGGAAGCGG CCGCAGCGCA TGTGATGGAG 
GTTGGCCCCA CCACCACGCT TTCGGCCCGT GCCGTGGAGC TTGTTCTGTC GGTGGGGCTC 
GTGCGTGCAG CCACACTGGA GAATCGACGC GCGCGCCGCC GCATGGAGAA GGCGGAGAAG 
GGAGACGGGA GCAGCGCCAG GGGCGATGGC AGCACATCGG GTGGCGGTGT GCTCGTGGGA 
GAGAGCGGGT CGCAGAAGTT GACCCCAGGC GGTGGAGCTG CCTCAGCGAT GATGGACAGC 
CCGGACGCGC CGCTCCCGTC GCTGGGCGGC GGTGGTGATG GAGGTGGATT TCCGACGGGT 
ACCATCGGCG GGGCAGGCGC GGCAGGGGAC GACGAGGGCG TCAATGTTCA ACTCGTGCCA 
CCAACCGCAC GTTGCCGCAG CGGCGGCGGC GACTTGAGCA TGCCTGAGCC TACCGCCTCG 
AGCGCTGTGG GCGATTTTGC GCACGCGAGC GGGGACGTCA AGGATACGGG CATGAGTGGC 
GGGCCCACAG ATCTGGGGTC CCAGCCCCTG TCACCGCGGT CGTCGTCATC GATCGGGTCC 
GCCTCTCAGC CGACGACAGG TGGTGGCGTC AAAGGCGCGC TCGCAGCGAA TCCAGCGCAG 
CACCAGGTTG GGGTGCGCGA CAAGCTGCCA GCGGACTGGG CCACCGCGCT GCGCGGCGTG 
GCGGAGCGGG TCGCGCGCGA CTTCATCGAC TACGCCTCCC GCCGCGTGCT GCTCGGCCAA 
GGTGAGGGCC AGTCGAACCA AGATCACCTG CGCACTGCGC GACGCATCCA CATCGACGCC 
CTCGAGGGCG TCGATGTGCC GGCGCTCTTC ACGCAAGAGC TGCAGCCGCG CGATGTGGCG 
CGGCTGACCT CCTACTACGT CGAGCTAGCG ACCAACGGGC CGCGTGACCG CGTCGACCCT 
CTCTGCGCCC CCGCCGCGCT TGGCGAGCAC AACATCTTCG GTCACCGCGG CAGCGTGAGA 
AACGAGGATG TCGCCAGCTT CGGCAACCTC GACGCAGCGT CGGCGAGCGT GTTTGCGCCA 
CCGACGCGGC CCCGGTCCCT CTTCGCATCC TCCCACCTCC TTCCATCGCA CAAAAACCAC 
CTGCTGTCGG CAGCCACAGC CATTCACGTG CGTCGCGCCG GACTGCTGCA TCTGGTGCTG 
GAGCAGTGCC TGTCGAACGT GCTGCATGAC CTGGTCGGGG ACACGGTGGG CTGGCTCTGG 
ACCGCCTGTC TGCAAGCAGC ACCGCCGGGA GCCGCACCGA ATGGCACGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_05_0530 cDNA ORF clone, Leishmania infantum JPCM5

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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