LMJF_15_0910 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	LMJF_15_0910
Entrez Gene ID	5650472
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_001681953.1	XP_001682005.1	conserved hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LMJF_15_0910 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_15_0910 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb01591	XM_001681953.1 Latest version!	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LMJF_15_0910 ( XM_001681953.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001682005.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OLb01591
Clone ID Related Accession (Same CDS sequence)	XM_001681953.1
Accession Version	XM_001681953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	conserved hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007256). COMPLETENESS: incomplete on both ends.

ATGCACGTGT CATCACCGCA GAGGGCAAAG TCTGAGGCCT CGCAGCCGCC CAACGGCAGC 
CCACCCCTCT CCTCCTCCGA GCCAGCGGCC GTACACCTGT ACGACCTCGA TACCGTCTTT 
CTCTCCAAGA CCGTCATCTC TTTGACCGAC GTCTTCACGA ACTCCACAGA TGGCCCCTTC 
GACCTGTCGC CTCTGTGCGC CCCGCAGCAG CAAGGCGTGG AGGAGCAGCA GCGGCTTCAA 
CAGGCAGCTG CCACAACAGC CTCGTCCCCC TCCACAGGTG ATGCGCACTG CTCGCCTTCA 
AGCGGTGCAA CACATGCGCA CATGTGCTCA ACAAGTCTGC CATTCTCTGG CGGATCGACG 
CTGCGACCGA AGGCCGTGGC ACGCGGCGCG GACGGACAGT GGCGCACCGT CTCTACTGAC 
CGCGGTGCGC CTGCGAGCTC CGCCTTCACA ACTGCCTGCA ACAAGCCGCC GTCGCAGGAG 
GCTCACGCAC GAATGCTGCT CGTCCCGCTC GACTCCTACC ACTTCCTCCT CACCTACCAC 
GACGTCGATG TGGCCGGGCA GTTGCGCGGC CGCTGCGACA AGCAGACCGC GATGGCGACC 
ATGGAGCGCC TCTCCGCCGC CTCGCCTGGA TGCCGCCCCC CGCGCAGCGG CATCTGTGTG 
GTGCGACGCT TAGAGGGGGA GCAGCGGGGC GCCACCAAGG CATCTTCAGC CGCCACGGCA 
GTGCTAGCAG GCGCCGCATC CGCAACGCGT CCCACCCTCT CCCGCATCGC GCACGGCCTG 
CCGCTGGACG CCTTCGATGG TTGGGTACGC GACTACACCG TCTACGGACT GTCGTGCAAC 
GAGGCGGTTC TGCGTGCGGC GTGGGAAGAC AAGGCGCGCG AGCGCGCCGA GGGCACTTTG 
GCAATGCCGG CTGGGGCCGT GGTGGAGGTT GGAGAGTCGG CGGCACCTGG ACACGAATCG 
GCAGGTGGAG GCGGAAGCGG CGAAGAGCGC CGTGTTACGG GCGGCGGCAA CAGTCGTGGC 
GACAATGTCG GTGGCGTCCA CATCAGTGTC CCGCCTGCAA GCGGTAGTAG CGCAGAGGCG 
ACGGTCGAAG GAGCGGCAGC ACTGGCGCCG CAGCTGTCTG ATGCGCCATC GGTGTTGGTG 
CCCACCGATG GGGCCGCGGT GGGGGAGGGC AAGGACGCGG CCACATTCGC ATCTCCTGAT 
GCAGCTAGAG CGGCGATGCT CTCCCCTTCG TTGGATCGAC TACACTGCAG CAGCACCACA 
AGCTCCTCAT CAACCGCCAC GCCAACCCCA TCGCCGCCCA CCGAAAGGAG CGAGGAGGGT 
GGCAAAGTCG GTGGCACCGT GTCAACCACG ATGTCAGCTT CACCGGCGTC CACCGCTGCA 
CAGCAGCAGC AGCAGCAGAA CGATGTGCTG TGGGAAGAAG AGCGACAGCG CACGCAGCGG 
GCGGCAGCGC AACTACGGCA CCGCTTCCTC GTCTCTGACA GTGCGCAGCA CGCCCTCTGG 
GCTCTGGAAG TGCATCTCCC TGCCATGAAG GTGCGCGCGG TTTCACCTGC GGAGTTCTAC 
GCGCTGGAGG TGGAAGAGAG CGACGAGGAG GAGGTGCCAG CAGCTGCCAG GCACAGCGAG 
GATGATGGCA ACGATGGTGA CCATGAAGCA TCGGCGACAC GCTGCGCTGC AGAAACACGA 
GAGGATCCTG CTGATGCACC TCCACCGGGA CTGGCGCTTC CTCGCAGGAA GACAACGTCT 
GCAATCACGC GCGGCCGCGC GGCCGTCTCA GGTCACCGCG ACTCTGTCAA GGGCACGCCC 
GGCACCGACG CATCGCGGCC GCGCTCGCCA TCGCCGGCAG ATCCGTTAGC GACCGCCGAC 
ACCGACGAAG CAGACCACCG TGGTGACTCC AGACCCGACG GCAGTGACGC GCGTCGCTCC 
TCCACGTCGG TGGCGACGCA CCACGCTGGT GCGAGCTGTA GCACACGGCG CTGCGGCCGG 
TCCGCCTCCT CGGCAAGTCC ATCGCTTGCC ATGGTGAAGG CGATGCCCAC GCCACTGATA 
GGCGGCGCGC GCGGCTTCGT CGATGGACAC TTCAGCGTTG CTCGCTTTAA TTCGCCGGGG 
TCGCTGTGCT GGCGCATCGA CGAAGACGAT GAGGACGACA GCGAGAAGGA CGTCGCTGGT 
GGTGGAAGCG AAAGCGGAGA TGCCATCGAC CATGCCGTGG CAGCAGCGTC GGAGGCGAAT 
CATGAGCTGG GAGGCCGGGC GCGCACCAGG CACGGCCGCG TCGACCCTGT TTCGTACCTT 
CGCCGTCGCC GCCGCTGCAC TGTTCTCTTC ATCAGCGACA TGGGCAACTG CGCGATTCGG 
TACGCCAACT TCCACAACCG CCTCGTGCGC ACCATCACGG GCGTGGACGG CGTCCCTGGC 
TACCGAGATG GCAGTTGCGT GTCGTCGCTC TTGCGTGGAG CGACAGCACT GGCCTGGTGC 
TCCGCTGGCC TGCTCTTCAC AGACGGGGCG AACAACGTCG TACGGCTTGT CACGAACATC 
CGCAAGCGTG GCAGCGGTGA GAGCACAACA GAAGAAAGTG CCGGCATCCC GCTAGCACGC 
AGCGCCACAC GCGCGACTAA GTCAGAGGTG CAAAGGCCGG GCGAGCTTGA GGTGCACCAC 
GAGACCTCGG CAGCAACAGA ACAGCCGCGC TGTGTCGGGG ACAAGCCGGC TGGCGACTCG 
CTCGACACCT GCCTTGCCAC AGCAACGGAG TCCCAGGACG GAGCGGTGCG AGAACTGCCG 
CCCACACCGC CGTCATCGGG CTCTCGAACA CCTACGCGTG AAGGGGCGCT GCCGTCATCG 
ACATGGAAGC GCGACGGCCC GGCGGCCGCG ATGCCGCGTG TTTGGACGCT GGCAGGTTGC 
ACAAGGACCA CCGATGCTGA TCAGCACGCA CACGCGGATG CAAGTCTGGC ATCCTACGTC 
GACTGCGCCG TCCCGTCGCG AGCACGTTTC GGCTACATCA GCGACATGGC CTTGTGGACC 
GACGAGACTG GCGACACGCA AGTGCTGCTA GTTGACCAGA CCCACCACGC CCTCCGCATC 
CTGGACGTAC ACGGCGGTGT CTCCACCTAC GTCGGGCCCT TGGACTACGA GGTGACGAGC 
ATGGCTTCGC CGTCAACGAC GAGCACCCCA GCGGCCGATT CCCTGCCACT AGGACTTGTG 
TTTCCGTGCT GCTTAACTGT TAGCGCGCTC ATCAAAGAGC GATCCCCGGC GCTGCTGAGC 
GCCGCCGCTA CCACCGCCGC TGCCACCATG ATCACCCCTC AGCCCTACCT CTGCTCGTCC 
AGCCCGCTGC TCTTCACGGC AAGCGCCATA ACAGGCGCCG TGTCGGTGCT GCTGCCCATC 
TCGCAGCGCA GTGCTCAGAC ACCTTGGAAC GTGCACGAGA ACCAGCACCG ACTCAAAGCC 
GCGGAGACTA GAGAAGGAGA CGGCACGTGC AACGGCGGCC ATGACGATGC CTCGATGTTC 
GCCACCATCC GTGGTGCGCT TGAGCTGGGC GTGGCCACCG AAGGTCGCCG CATCATCGGC 
GAAGCCGCCG CGCCCTCTCT CAAGCACGTG GTGGTAGGGT GGGCCGCGGA CGAGGCTGAC 
GGCGACGGGG TGGAGAGCGC ACGCGCAGCG CGTCGGCTGT TGCGGGTGGC GAGTGCGCAG 
CAAGCCTCGC AGTATCTTCG GCTGCGCTTC CCGTGGCTAC TGCCGCCGTC CGTGCAGCAG 
CTGAGTACGC GCCTCGTTGC TGGCTGCACC TGTCATGTAT CCGGCAAGCG TCGCGGCGCC 
GCAGCCGCCG CCCCGGCAGC TGCACGCAAC GCGGTAGGTC TTCCTTGCAA CACCGGCAGC 
GACCGCCAAG GCGTTGGTGC GTCCTCGGGT GTACCGCCGA ACTGCACCAA GCGGGAAAGG 
GGAGGCGTGG CCACGGCAAC TTCGACCTCG CAGCAGCGGC GACGAGTGCT CTTTCAAAGT 
CCCCCGCGCC ATCCTCCGAT CATGACGCCC CGGTGCCCAC CAGCCCAAAC AGACGCAGCC 
GTGGCCGCAG CGGCGAAGGA GTTGGGGGCG CTGTTGCTCG AGCCGGCCCT GCTTTCCGCT 
ACACCACCGC GCATGGTGGA GGTCCTCGAC ACGCACCTCG GTGTCGGTGG TGATGGTGTT 
GCGGCAGGCG ATCCAGAGGC GCTTTATGCG AGTCGCAGCA GCCCACCCCG TGCCGTGTCG 
CCGCGGAGCT CCGACATCGA CCCCAACATC GAGGAGGGCG AAGGCGGCTC GCGACGAAGC 
AGCACCAGCA GCAACCCTCT GCAGCCGGAG GGTAACGAAG AGGAGGAGCA GAAGGCTGTC 
GGTGGTGATG TGGGCGGTGG CCGCGCGGCG GCGAAGTGGC TGGCACCACC GCTGAAGAGC 
GTCTCTCGCT CCCCGAGCGG CAGCAGAAGC GGGGGACAGC AGCAGCCTAT CGACCCTGCC 
ACGTCTCCCT TAGCGCCGCC TTCGCTCACC TACGCCGGGC GATTCCAAGA GTGGACGCCA 
GAGCAGCGCC AACAGCGTCT GCGCAGCACG CAAGAGATCC CCATCTCCAC GACGAGCGTC 
CGCAGAAGCC CATGTGGGTC ACCGTGTGCG CTGCACAAGC AGCAGCAGCA GCAGCAACAA 
CAAGGGGAAG AGGATGATGG CAGTCCTTCA CAATTGTGCC AGTCCGCAGC CCGTGGGCCG 
CCGCCGCTGT CGTCGTCGCG CCCGCGTGGC GAGCGAGCCG CATCCCCAAA CAACCTGAGC 
GGTCGCGGCA GCCCACCGCT GACGCCGCGA GCGATGGTGG CGCACCAGCT CATGCAGCGC 
CGCCGCACCA GTCGAAGTAG CACTGCCCCG CATGCTGCAT CGGTAGAGGG TGGTGCGAGA 
GGGTCAATCA AGTGTAAAAG AACGGCCGCC GCAGAGGTCT ATGATGACAT CAGCCCAATA 
GCGCAGGCGC CGCAGCAGCC GGAAGGCTCG CAAGGTCGTG CCGCGCCTGT GGAACCACTA 
GCGATGCCAG CTGTCCCTGA TCGACACCTC CTGCAGCTGT ATTGCGCAGA TCGGCTGTTG 
CACGACGCCT ACGATGCGGC AGTGCGTCGA CTGTTCCGTG TCTACAGTTA TCTGGCGACC 
AAGATCGTTA CGACGACGAC GACAACGACA AGGAGCGCTG CACCTTCAGC AGCAGGAGTC 
GGTGGAAGTG CGGAGCCGCG AATCGCTGCG GTGGGAAGTC GCCGTGGCAG AGCGCCGTCG 
CCGTGGGCCA CGCACCGGCC TCGCCAGGTG GAGCAGTACA CCATGTCCTT TGCGGCCTTC 
TTTCGATTCG TCGTGCTCAC CGGATACGCC GACTACCTCG CCGAGGTGGC CGTTGCGGCG 
GTCACCGCGG CGGCGACGGG CGCGCCAGGC AAAGACAGCG CCGGTGCTCA CGCAGAAGCC 
CGTGCGAGCA GCTCGCCGTC ACCGGTGTCG CTGATGTGTC GACTTAGCCA AGTGCAACAG 
CGGCGAGGCG GCAGCAGCTG CGGCGCCGGG ACACGGCGAT GGAGTAGCGC ATCCGCGTCC 
CCGCCGACAG CGCCACCCGG GGCGGCAACC GCAGTGACAT CGTCGACCTC GCCTTCCAGG 
CACCCTACCC CCTCCGACGT GCTAGTGGTG CCGCTGGCCA CCACTGACGC CCGCGCCATT 
GCGGCGGTGC TGTACGCGTG CGGTGTGCGC CAGAAGGGCT ACCATACATT GACGCAGATG 
GACTTCCAGA GTTTTCGAAG AGCCGCGCTG CTGCTCCACA CGTGGACGCG TACGGCGACC 
TGCGCAGAAG CGGCGAGGAT GAGCGGGACA GCGGACGATG ACGGTGTGCA CAGCGGCCGA 
TGCAGCGCTC ACCGCCAGGC TAGCAGGCGG AACGGCACGG ATGCCGCCGC CTTCGCCTAC 
GTGGACACCG TTCCCTCACT AGATGCCCTC ACGTCGGAGG AGGTGGTGGT TGCCTACGCG 
GCCGTGTACG AGCGGGCTGT CCGCTGCATC CCGGCCCTGG CGATGTCGTT TGCAGCAGAC 
GGCACCAGCA GAGAGGAGGA GGCGAACCGC AACGTGGCCG AAGCGCGCGA CGAGGCCAGG 
CAGCGTAGCG CTGTGTCGCG GCAAGGCCGC CCAGCGAGCT CGATGCGCAC ATCAGACAGA 
GCCCCATCAC TGCTCGTGGC TGTGGGCACT GGCTCGCCAG GCGCGACGTC GTCGCACCCA 
AGCAGTTCCG AGGCTGCGAT GGGTGGCAGT CATCACAGTG GCGGCGGTGC TGGTGGCAAA 
GCGGCAGGCG TAGATGAGGC CGTCACCCCG CCTGTGTCGC CCATCGCCAC CGCAGGAGCT 
GCCGATGACT TGTCCTGTTT CGCCCTAGAT GGTGGTGACG TAGCCATTCT CTTCCCACCC 
GGCGGGCTTA CACCGCACGA AACCCTCGCC CTCGATGAGC TTCTGCGCCT CCTGCAGCGC 
AACGAATCGA CCCTGCGGCA GCTCTTTGAG GCTTACAGCG TGCCAATCAC CGTGCACCGC 
TCACCGCAGT ACGAGGCTCC GTCGTCCGCA GCAGCCGCAA CCTCGAGCCC GAGCTCATTG 
CTCACGAGTA GTCCTGAACT CTCGCTGGTC CTCGGCGACG GCGGGACGGC AGTGGCACGG 
CAGCCGCGAC ACACGTCAGG GAAGCCGTTG GCGTCGGTAC ATGTCGACGC ATCACCAGGA 
GCTGCACGCC ATCCGGTGGT CGCGCACCGC AATCGCCAGT CTGCGGCCAG AGCTCGCGCC 
AAGTCGTCTG CAATGGCCGC TGCGGCAACA GCAGTAGCAC CGCTGGACGT TGTGCACGAC 
TCCACCGACC TGTCTGGCGT CTCGGCACGG CAGCAAGACG TATCATGGAA GGTGCAGCAG 
CTCTACACCA TGAGCGCTGT CGAGAACAAG GAAGCCTACG AGCGCACGAG TCGCGTGCTG 
CGTGTGATGC CCTTTAAGCT GTTTGTAGAT CTCTGGCGCA CACTGGACGT GTTCCCTTCG 
CTGATGGGCA CGGGGGCGAT GCAGCAGGTG TTTGGCGATG CGCTCACCAC GCCCTTGCTT 
CGCGGCTTAA CGTCGTCACC AGGGAACACA TCGGGCGCGC TGCTTCAAGC GCAAGAGCAG 
CCACCACGAC CGTCCTGCAC TCGCTCTGAT GTCCTACCCA GCAGACGCAC CGTCGAGCTG 
TTGTGCGCGC ATGGCGGGCT GCCCTACGCC TGCTTCGTCG AGTCCTTTGT CCGTGTGGCC 
CTCGTCGTCT TCTCCCATAC GGTGGATCGG ATTGCCTACC CGACGGCCAC GGCGAAGACT 
GCCGGATTAA TGCAGTGGTG CAACAAGCAA GTCACTCTAG GCCTAGTGGC GAAGCGAGTG 
CAGCAGCAAC TCCGTGTCCT GGTAGCCGGG GGGCGCGGAT CGAGCAGGAG AGCCTCGATG 
GCGCCGTCTG CAGCAGCGGC AGCGGCATCA GCGTCATGTG CAACGCGAGC CGTGGGCGTC 
TTTCCCGACC AGCTGCGGCT CTTCCGCGTA CCAACAGCGC CAAAGGCGAG CACCGCACCA 
CCAGGGACAG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001682005.1
CDS	1..7515
Translation	MHVSSPQRAKSEASQPPNGSPPLSSSEPAAVHLYDLDTVFLSKTVISLTDVFTNSTDGPFDLSPLCAPQQQGVEEQQRLQQAAATTASSPSTGDAHCSPSSGATHAHMCSTSLPFSGGSTLRPKAVARGADGQWRTVSTDRGAPASSAFTTACNKPPSQEAHARMLLVPLDSYHFLLTYHDVDVAGQLRGRCDKQTAMATMERLSAASPGCRPPRSGICVVRRLEGEQRGATKASSAATAVLAGAASATRPTLSRIAHGLPLDAFDGWVRDYTVYGLSCNEAVLRAAWEDKARERAEGTLAMPAGAVVEVGESAAPGHESAGGGGSGEERRVTGGGNSRGDNVGGVHISVPPASGSSAEATVEGAAALAPQLSDAPSVLVPTDGAAVGEGKDAATFASPDAARAAMLSPSLDRLHCSSTTSSSSTATPTPSPPTERSEEGGKVGGTVSTTMSASPASTAAQQQQQQNDVLWEEERQRTQRAAAQLRHRFLVSDSAQHALWALEVHLPAMKVRAVSPAEFYALEVEESDEEEVPAAARHSEDDGNDGDHEASATRCAAETREDPADAPPPGLALPRRKTTSAITRGRAAVSGHRDSVKGTPGTDASRPRSPSPADPLATADTDEADHRGDSRPDGSDARRSSTSVATHHAGASCSTRRCGRSASSASPSLAMVKAMPTPLIGGARGFVDGHFSVARFNSPGSLCWRIDEDDEDDSEKDVAGGGSESGDAIDHAVAAASEANHELGGRARTRHGRVDPVSYLRRRRRCTVLFISDMGNCAIRYANFHNRLVRTITGVDGVPGYRDGSCVSSLLRGATALAWCSAGLLFTDGANNVVRLVTNIRKRGSGESTTEESAGIPLARSATRATKSEVQRPGELEVHHETSAATEQPRCVGDKPAGDSLDTCLATATESQDGAVRELPPTPPSSGSRTPTREGALPSSTWKRDGPAAAMPRVWTLAGCTRTTDADQHAHADASLASYVDCAVPSRARFGYISDMALWTDETGDTQVLLVDQTHHALRILDVHGGVSTYVGPLDYEVTSMASPSTTSTPAADSLPLGLVFPCCLTVSALIKERSPALLSAAATTAAATMITPQPYLCSSSPLLFTASAITGAVSVLLPISQRSAQTPWNVHENQHRLKAAETREGDGTCNGGHDDASMFATIRGALELGVATEGRRIIGEAAAPSLKHVVVGWAADEADGDGVESARAARRLLRVASAQQASQYLRLRFPWLLPPSVQQLSTRLVAGCTCHVSGKRRGAAAAAPAAARNAVGLPCNTGSDRQGVGASSGVPPNCTKRERGGVATATSTSQQRRRVLFQSPPRHPPIMTPRCPPAQTDAAVAAAAKELGALLLEPALLSATPPRMVEVLDTHLGVGGDGVAAGDPEALYASRSSPPRAVSPRSSDIDPNIEEGEGGSRRSSTSSNPLQPEGNEEEEQKAVGGDVGGGRAAAKWLAPPLKSVSRSPSGSRSGGQQQPIDPATSPLAPPSLTYAGRFQEWTPEQRQQRLRSTQEIPISTTSVRRSPCGSPCALHKQQQQQQQQGEEDDGSPSQLCQSAARGPPPLSSSRPRGERAASPNNLSGRGSPPLTPRAMVAHQLMQRRRTSRSSTAPHAASVEGGARGSIKCKRTAAAEVYDDISPIAQAPQQPEGSQGRAAPVEPLAMPAVPDRHLLQLYCADRLLHDAYDAAVRRLFRVYSYLATKIVTTTTTTTRSAAPSAAGVGGSAEPRIAAVGSRRGRAPSPWATHRPRQVEQYTMSFAAFFRFVVLTGYADYLAEVAVAAVTAAATGAPGKDSAGAHAEARASSSPSPVSLMCRLSQVQQRRGGSSCGAGTRRWSSASASPPTAPPGAATAVTSSTSPSRHPTPSDVLVVPLATTDARAIAAVLYACGVRQKGYHTLTQMDFQSFRRAALLLHTWTRTATCAEAARMSGTADDDGVHSGRCSAHRQASRRNGTDAAAFAYVDTVPSLDALTSEEVVVAYAAVYERAVRCIPALAMSFAADGTSREEEANRNVAEARDEARQRSAVSRQGRPASSMRTSDRAPSLLVAVGTGSPGATSSHPSSSEAAMGGSHHSGGGAGGKAAGVDEAVTPPVSPIATAGAADDLSCFALDGGDVAILFPPGGLTPHETLALDELLRLLQRNESTLRQLFEAYSVPITVHRSPQYEAPSSAAAATSSPSSLLTSSPELSLVLGDGGTAVARQPRHTSGKPLASVHVDASPGAARHPVVAHRNRQSAARARAKSSAMAAAATAVAPLDVVHDSTDLSGVSARQQDVSWKVQQLYTMSAVENKEAYERTSRVLRVMPFKLFVDLWRTLDVFPSLMGTGAMQQVFGDALTTPLLRGLTSSPGNTSGALLQAQEQPPRPSCTRSDVLPSRRTVELLCAHGGLPYACFVESFVRVALVVFSHTVDRIAYPTATAKTAGLMQWCNKQVTLGLVAKRVQQQLRVLVAGGRGSSRRASMAPSAAAAAASASCATRAVGVFPDQLRLFRVPTAPKASTAPPGTG

Target ORF information:

RefSeq Version	XM_001681953.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001681953.1

ORF Insert Sequence:

ATGCACGTGT CATCACCGCA GAGGGCAAAG TCTGAGGCCT CGCAGCCGCC CAACGGCAGC 
CCACCCCTCT CCTCCTCCGA GCCAGCGGCC GTACACCTGT ACGACCTCGA TACCGTCTTT 
CTCTCCAAGA CCGTCATCTC TTTGACCGAC GTCTTCACGA ACTCCACAGA TGGCCCCTTC 
GACCTGTCGC CTCTGTGCGC CCCGCAGCAG CAAGGCGTGG AGGAGCAGCA GCGGCTTCAA 
CAGGCAGCTG CCACAACAGC CTCGTCCCCC TCCACAGGTG ATGCGCACTG CTCGCCTTCA 
AGCGGTGCAA CACATGCGCA CATGTGCTCA ACAAGTCTGC CATTCTCTGG CGGATCGACG 
CTGCGACCGA AGGCCGTGGC ACGCGGCGCG GACGGACAGT GGCGCACCGT CTCTACTGAC 
CGCGGTGCGC CTGCGAGCTC CGCCTTCACA ACTGCCTGCA ACAAGCCGCC GTCGCAGGAG 
GCTCACGCAC GAATGCTGCT CGTCCCGCTC GACTCCTACC ACTTCCTCCT CACCTACCAC 
GACGTCGATG TGGCCGGGCA GTTGCGCGGC CGCTGCGACA AGCAGACCGC GATGGCGACC 
ATGGAGCGCC TCTCCGCCGC CTCGCCTGGA TGCCGCCCCC CGCGCAGCGG CATCTGTGTG 
GTGCGACGCT TAGAGGGGGA GCAGCGGGGC GCCACCAAGG CATCTTCAGC CGCCACGGCA 
GTGCTAGCAG GCGCCGCATC CGCAACGCGT CCCACCCTCT CCCGCATCGC GCACGGCCTG 
CCGCTGGACG CCTTCGATGG TTGGGTACGC GACTACACCG TCTACGGACT GTCGTGCAAC 
GAGGCGGTTC TGCGTGCGGC GTGGGAAGAC AAGGCGCGCG AGCGCGCCGA GGGCACTTTG 
GCAATGCCGG CTGGGGCCGT GGTGGAGGTT GGAGAGTCGG CGGCACCTGG ACACGAATCG 
GCAGGTGGAG GCGGAAGCGG CGAAGAGCGC CGTGTTACGG GCGGCGGCAA CAGTCGTGGC 
GACAATGTCG GTGGCGTCCA CATCAGTGTC CCGCCTGCAA GCGGTAGTAG CGCAGAGGCG 
ACGGTCGAAG GAGCGGCAGC ACTGGCGCCG CAGCTGTCTG ATGCGCCATC GGTGTTGGTG 
CCCACCGATG GGGCCGCGGT GGGGGAGGGC AAGGACGCGG CCACATTCGC ATCTCCTGAT 
GCAGCTAGAG CGGCGATGCT CTCCCCTTCG TTGGATCGAC TACACTGCAG CAGCACCACA 
AGCTCCTCAT CAACCGCCAC GCCAACCCCA TCGCCGCCCA CCGAAAGGAG CGAGGAGGGT 
GGCAAAGTCG GTGGCACCGT GTCAACCACG ATGTCAGCTT CACCGGCGTC CACCGCTGCA 
CAGCAGCAGC AGCAGCAGAA CGATGTGCTG TGGGAAGAAG AGCGACAGCG CACGCAGCGG 
GCGGCAGCGC AACTACGGCA CCGCTTCCTC GTCTCTGACA GTGCGCAGCA CGCCCTCTGG 
GCTCTGGAAG TGCATCTCCC TGCCATGAAG GTGCGCGCGG TTTCACCTGC GGAGTTCTAC 
GCGCTGGAGG TGGAAGAGAG CGACGAGGAG GAGGTGCCAG CAGCTGCCAG GCACAGCGAG 
GATGATGGCA ACGATGGTGA CCATGAAGCA TCGGCGACAC GCTGCGCTGC AGAAACACGA 
GAGGATCCTG CTGATGCACC TCCACCGGGA CTGGCGCTTC CTCGCAGGAA GACAACGTCT 
GCAATCACGC GCGGCCGCGC GGCCGTCTCA GGTCACCGCG ACTCTGTCAA GGGCACGCCC 
GGCACCGACG CATCGCGGCC GCGCTCGCCA TCGCCGGCAG ATCCGTTAGC GACCGCCGAC 
ACCGACGAAG CAGACCACCG TGGTGACTCC AGACCCGACG GCAGTGACGC GCGTCGCTCC 
TCCACGTCGG TGGCGACGCA CCACGCTGGT GCGAGCTGTA GCACACGGCG CTGCGGCCGG 
TCCGCCTCCT CGGCAAGTCC ATCGCTTGCC ATGGTGAAGG CGATGCCCAC GCCACTGATA 
GGCGGCGCGC GCGGCTTCGT CGATGGACAC TTCAGCGTTG CTCGCTTTAA TTCGCCGGGG 
TCGCTGTGCT GGCGCATCGA CGAAGACGAT GAGGACGACA GCGAGAAGGA CGTCGCTGGT 
GGTGGAAGCG AAAGCGGAGA TGCCATCGAC CATGCCGTGG CAGCAGCGTC GGAGGCGAAT 
CATGAGCTGG GAGGCCGGGC GCGCACCAGG CACGGCCGCG TCGACCCTGT TTCGTACCTT 
CGCCGTCGCC GCCGCTGCAC TGTTCTCTTC ATCAGCGACA TGGGCAACTG CGCGATTCGG 
TACGCCAACT TCCACAACCG CCTCGTGCGC ACCATCACGG GCGTGGACGG CGTCCCTGGC 
TACCGAGATG GCAGTTGCGT GTCGTCGCTC TTGCGTGGAG CGACAGCACT GGCCTGGTGC 
TCCGCTGGCC TGCTCTTCAC AGACGGGGCG AACAACGTCG TACGGCTTGT CACGAACATC 
CGCAAGCGTG GCAGCGGTGA GAGCACAACA GAAGAAAGTG CCGGCATCCC GCTAGCACGC 
AGCGCCACAC GCGCGACTAA GTCAGAGGTG CAAAGGCCGG GCGAGCTTGA GGTGCACCAC 
GAGACCTCGG CAGCAACAGA ACAGCCGCGC TGTGTCGGGG ACAAGCCGGC TGGCGACTCG 
CTCGACACCT GCCTTGCCAC AGCAACGGAG TCCCAGGACG GAGCGGTGCG AGAACTGCCG 
CCCACACCGC CGTCATCGGG CTCTCGAACA CCTACGCGTG AAGGGGCGCT GCCGTCATCG 
ACATGGAAGC GCGACGGCCC GGCGGCCGCG ATGCCGCGTG TTTGGACGCT GGCAGGTTGC 
ACAAGGACCA CCGATGCTGA TCAGCACGCA CACGCGGATG CAAGTCTGGC ATCCTACGTC 
GACTGCGCCG TCCCGTCGCG AGCACGTTTC GGCTACATCA GCGACATGGC CTTGTGGACC 
GACGAGACTG GCGACACGCA AGTGCTGCTA GTTGACCAGA CCCACCACGC CCTCCGCATC 
CTGGACGTAC ACGGCGGTGT CTCCACCTAC GTCGGGCCCT TGGACTACGA GGTGACGAGC 
ATGGCTTCGC CGTCAACGAC GAGCACCCCA GCGGCCGATT CCCTGCCACT AGGACTTGTG 
TTTCCGTGCT GCTTAACTGT TAGCGCGCTC ATCAAAGAGC GATCCCCGGC GCTGCTGAGC 
GCCGCCGCTA CCACCGCCGC TGCCACCATG ATCACCCCTC AGCCCTACCT CTGCTCGTCC 
AGCCCGCTGC TCTTCACGGC AAGCGCCATA ACAGGCGCCG TGTCGGTGCT GCTGCCCATC 
TCGCAGCGCA GTGCTCAGAC ACCTTGGAAC GTGCACGAGA ACCAGCACCG ACTCAAAGCC 
GCGGAGACTA GAGAAGGAGA CGGCACGTGC AACGGCGGCC ATGACGATGC CTCGATGTTC 
GCCACCATCC GTGGTGCGCT TGAGCTGGGC GTGGCCACCG AAGGTCGCCG CATCATCGGC 
GAAGCCGCCG CGCCCTCTCT CAAGCACGTG GTGGTAGGGT GGGCCGCGGA CGAGGCTGAC 
GGCGACGGGG TGGAGAGCGC ACGCGCAGCG CGTCGGCTGT TGCGGGTGGC GAGTGCGCAG 
CAAGCCTCGC AGTATCTTCG GCTGCGCTTC CCGTGGCTAC TGCCGCCGTC CGTGCAGCAG 
CTGAGTACGC GCCTCGTTGC TGGCTGCACC TGTCATGTAT CCGGCAAGCG TCGCGGCGCC 
GCAGCCGCCG CCCCGGCAGC TGCACGCAAC GCGGTAGGTC TTCCTTGCAA CACCGGCAGC 
GACCGCCAAG GCGTTGGTGC GTCCTCGGGT GTACCGCCGA ACTGCACCAA GCGGGAAAGG 
GGAGGCGTGG CCACGGCAAC TTCGACCTCG CAGCAGCGGC GACGAGTGCT CTTTCAAAGT 
CCCCCGCGCC ATCCTCCGAT CATGACGCCC CGGTGCCCAC CAGCCCAAAC AGACGCAGCC 
GTGGCCGCAG CGGCGAAGGA GTTGGGGGCG CTGTTGCTCG AGCCGGCCCT GCTTTCCGCT 
ACACCACCGC GCATGGTGGA GGTCCTCGAC ACGCACCTCG GTGTCGGTGG TGATGGTGTT 
GCGGCAGGCG ATCCAGAGGC GCTTTATGCG AGTCGCAGCA GCCCACCCCG TGCCGTGTCG 
CCGCGGAGCT CCGACATCGA CCCCAACATC GAGGAGGGCG AAGGCGGCTC GCGACGAAGC 
AGCACCAGCA GCAACCCTCT GCAGCCGGAG GGTAACGAAG AGGAGGAGCA GAAGGCTGTC 
GGTGGTGATG TGGGCGGTGG CCGCGCGGCG GCGAAGTGGC TGGCACCACC GCTGAAGAGC 
GTCTCTCGCT CCCCGAGCGG CAGCAGAAGC GGGGGACAGC AGCAGCCTAT CGACCCTGCC 
ACGTCTCCCT TAGCGCCGCC TTCGCTCACC TACGCCGGGC GATTCCAAGA GTGGACGCCA 
GAGCAGCGCC AACAGCGTCT GCGCAGCACG CAAGAGATCC CCATCTCCAC GACGAGCGTC 
CGCAGAAGCC CATGTGGGTC ACCGTGTGCG CTGCACAAGC AGCAGCAGCA GCAGCAACAA 
CAAGGGGAAG AGGATGATGG CAGTCCTTCA CAATTGTGCC AGTCCGCAGC CCGTGGGCCG 
CCGCCGCTGT CGTCGTCGCG CCCGCGTGGC GAGCGAGCCG CATCCCCAAA CAACCTGAGC 
GGTCGCGGCA GCCCACCGCT GACGCCGCGA GCGATGGTGG CGCACCAGCT CATGCAGCGC 
CGCCGCACCA GTCGAAGTAG CACTGCCCCG CATGCTGCAT CGGTAGAGGG TGGTGCGAGA 
GGGTCAATCA AGTGTAAAAG AACGGCCGCC GCAGAGGTCT ATGATGACAT CAGCCCAATA 
GCGCAGGCGC CGCAGCAGCC GGAAGGCTCG CAAGGTCGTG CCGCGCCTGT GGAACCACTA 
GCGATGCCAG CTGTCCCTGA TCGACACCTC CTGCAGCTGT ATTGCGCAGA TCGGCTGTTG 
CACGACGCCT ACGATGCGGC AGTGCGTCGA CTGTTCCGTG TCTACAGTTA TCTGGCGACC 
AAGATCGTTA CGACGACGAC GACAACGACA AGGAGCGCTG CACCTTCAGC AGCAGGAGTC 
GGTGGAAGTG CGGAGCCGCG AATCGCTGCG GTGGGAAGTC GCCGTGGCAG AGCGCCGTCG 
CCGTGGGCCA CGCACCGGCC TCGCCAGGTG GAGCAGTACA CCATGTCCTT TGCGGCCTTC 
TTTCGATTCG TCGTGCTCAC CGGATACGCC GACTACCTCG CCGAGGTGGC CGTTGCGGCG 
GTCACCGCGG CGGCGACGGG CGCGCCAGGC AAAGACAGCG CCGGTGCTCA CGCAGAAGCC 
CGTGCGAGCA GCTCGCCGTC ACCGGTGTCG CTGATGTGTC GACTTAGCCA AGTGCAACAG 
CGGCGAGGCG GCAGCAGCTG CGGCGCCGGG ACACGGCGAT GGAGTAGCGC ATCCGCGTCC 
CCGCCGACAG CGCCACCCGG GGCGGCAACC GCAGTGACAT CGTCGACCTC GCCTTCCAGG 
CACCCTACCC CCTCCGACGT GCTAGTGGTG CCGCTGGCCA CCACTGACGC CCGCGCCATT 
GCGGCGGTGC TGTACGCGTG CGGTGTGCGC CAGAAGGGCT ACCATACATT GACGCAGATG 
GACTTCCAGA GTTTTCGAAG AGCCGCGCTG CTGCTCCACA CGTGGACGCG TACGGCGACC 
TGCGCAGAAG CGGCGAGGAT GAGCGGGACA GCGGACGATG ACGGTGTGCA CAGCGGCCGA 
TGCAGCGCTC ACCGCCAGGC TAGCAGGCGG AACGGCACGG ATGCCGCCGC CTTCGCCTAC 
GTGGACACCG TTCCCTCACT AGATGCCCTC ACGTCGGAGG AGGTGGTGGT TGCCTACGCG 
GCCGTGTACG AGCGGGCTGT CCGCTGCATC CCGGCCCTGG CGATGTCGTT TGCAGCAGAC 
GGCACCAGCA GAGAGGAGGA GGCGAACCGC AACGTGGCCG AAGCGCGCGA CGAGGCCAGG 
CAGCGTAGCG CTGTGTCGCG GCAAGGCCGC CCAGCGAGCT CGATGCGCAC ATCAGACAGA 
GCCCCATCAC TGCTCGTGGC TGTGGGCACT GGCTCGCCAG GCGCGACGTC GTCGCACCCA 
AGCAGTTCCG AGGCTGCGAT GGGTGGCAGT CATCACAGTG GCGGCGGTGC TGGTGGCAAA 
GCGGCAGGCG TAGATGAGGC CGTCACCCCG CCTGTGTCGC CCATCGCCAC CGCAGGAGCT 
GCCGATGACT TGTCCTGTTT CGCCCTAGAT GGTGGTGACG TAGCCATTCT CTTCCCACCC 
GGCGGGCTTA CACCGCACGA AACCCTCGCC CTCGATGAGC TTCTGCGCCT CCTGCAGCGC 
AACGAATCGA CCCTGCGGCA GCTCTTTGAG GCTTACAGCG TGCCAATCAC CGTGCACCGC 
TCACCGCAGT ACGAGGCTCC GTCGTCCGCA GCAGCCGCAA CCTCGAGCCC GAGCTCATTG 
CTCACGAGTA GTCCTGAACT CTCGCTGGTC CTCGGCGACG GCGGGACGGC AGTGGCACGG 
CAGCCGCGAC ACACGTCAGG GAAGCCGTTG GCGTCGGTAC ATGTCGACGC ATCACCAGGA 
GCTGCACGCC ATCCGGTGGT CGCGCACCGC AATCGCCAGT CTGCGGCCAG AGCTCGCGCC 
AAGTCGTCTG CAATGGCCGC TGCGGCAACA GCAGTAGCAC CGCTGGACGT TGTGCACGAC 
TCCACCGACC TGTCTGGCGT CTCGGCACGG CAGCAAGACG TATCATGGAA GGTGCAGCAG 
CTCTACACCA TGAGCGCTGT CGAGAACAAG GAAGCCTACG AGCGCACGAG TCGCGTGCTG 
CGTGTGATGC CCTTTAAGCT GTTTGTAGAT CTCTGGCGCA CACTGGACGT GTTCCCTTCG 
CTGATGGGCA CGGGGGCGAT GCAGCAGGTG TTTGGCGATG CGCTCACCAC GCCCTTGCTT 
CGCGGCTTAA CGTCGTCACC AGGGAACACA TCGGGCGCGC TGCTTCAAGC GCAAGAGCAG 
CCACCACGAC CGTCCTGCAC TCGCTCTGAT GTCCTACCCA GCAGACGCAC CGTCGAGCTG 
TTGTGCGCGC ATGGCGGGCT GCCCTACGCC TGCTTCGTCG AGTCCTTTGT CCGTGTGGCC 
CTCGTCGTCT TCTCCCATAC GGTGGATCGG ATTGCCTACC CGACGGCCAC GGCGAAGACT 
GCCGGATTAA TGCAGTGGTG CAACAAGCAA GTCACTCTAG GCCTAGTGGC GAAGCGAGTG 
CAGCAGCAAC TCCGTGTCCT GGTAGCCGGG GGGCGCGGAT CGAGCAGGAG AGCCTCGATG 
GCGCCGTCTG CAGCAGCGGC AGCGGCATCA GCGTCATGTG CAACGCGAGC CGTGGGCGTC 
TTTCCCGACC AGCTGCGGCT CTTCCGCGTA CCAACAGCGC CAAAGGCGAG CACCGCACCA 
CCAGGGACAG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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LMJF_15_0910 cDNA ORF clone, Leishmania major strain Friedlin

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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