LMJF_05_0890 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	LMJF_05_0890
Entrez Gene ID	5648951
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_001687514.1	XP_001687566.1	conserved hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LMJF_05_0890 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_05_0890 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb00208	XM_001687514.1 Latest version!	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LMJF_05_0890 ( XM_001687514.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001687566.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OLb00208
Clone ID Related Accession (Same CDS sequence)	XM_001687514.1
Accession Version	XM_001687514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	conserved hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007246). COMPLETENESS: incomplete on both ends.

ATGAACCACA ACAGCGTGGC GGAGGGAGAA GATGGCGGCA CCGGTGCAGA CGCCGCCGCC 
GTCGTGCCCG TGAACGAGAC GCTGCTGTAC CACCAGACCT TGATCCCCAC ACAGAATGTC 
ACCCACGCCA TCGAGGGCTC CTTCACGCTG CCGGACGCGA ACGACCTCGT TCTGATCCGT 
CACAACGTAC TCGAGCTGTG GCGACTTTAC GCAGAGAACG CCAACGGCGG CGTAGAGTGT 
GTTTGCCGCA CCCCGTTGAT GTCGGCTGTG TACGCTGCGG TGGCGGTGCC GACAAGCGCC 
TCGTCGATGG CGAAGGCCGG TGGCACGGGC AGTGCCGCGT CGCCGGGCGG AAGTGTTGCC 
CCTGTACTCG ACGACGCGAC GACCACAGCG CACCGCAGTG GGGTACACTA CCTCGCCGTC 
ACGAGTGAGA CGGGCTACGT GACACTGCTG CGGTACCAGC TGGACGAGCC GCCGGTGCCG 
ACGCGGCTGT CCTACGGCGA CGACAGCGAC GAGGGCAACG AAGGTGTTGG TGGGAACAGT 
GGCGCTAGCG GCGGTGCCAC GATGCTCGTG ACCACGTCCT TGAAGGGCCG CTTCATCAAG 
GTCAGCGAGG TGTGCCTGGG CCGGAGCGGG GCGCGGCTGA CCGTCCCTGG TGCCCGCATG 
GTGGTCGACC CCGCCGGCAC GGCGCTCTTG ATCACCGCGC TCATGCGTAC CAAAACGTTG 
GTGCCCATCA CCGCGAATGA GACCTGGGAT CCGGTCAAGA GCGTCAACGT CGGACAAGAC 
GACGACGACG ACGCAGAGAG CGACGCTGAC GGCGATGACG AGGACGAAGA CGGTGACGAC 
GACGATGCTG GCGAGCACCA GCGCCGCGGC CCGGTGACAG GTCGAGAGCT TCGTGCAGCC 
CGGCGCCGTC GGGCGGCCAA AGTGCGACGG GCCAACGGCG CCATCAACCT CAGCACGCCA 
ATCGAGATGC AGCGCCAGAC CGTCCTGTAC TCCGCCTGCG CCCTTGATGG CTACCTCGAC 
CATGCTGTGT TCGCCGTGCT CGAGGAGGAG ATCGTCGAAG CACGCGACGC CGCCGCCGCG 
GCGTCCGCCA GTAACCCCGC GAACGCCGCG GCAGCCGCAG CCGAGGCGAG CGGTGGCACC 
TTCCTCAAGA GCAGCGGAGG CCCGAACGCG GTGGTTCAGC GCCACAAGCA CTTCGTGCTC 
TACGCCTACG TGCCGAGCCT GAAGCAGGTG CAGCGGACGC AGCTTGTGCA CGTTCCAGTG 
ACGGCGCACC GTCTCATCGC TGTCCCGGCC GCACCGTACG GCCCAGGCGG CGTTCTTGTC 
TGCACAGACA CGGAGCTGAT CTGGTACGAT GTGTCCGCCA CGCAACAGCA GCAGCAGCAG 
AACTCCGCTG CGGCGGCGAA TGGCCGCGTC GGCGTCTTCA AGTGCACCTA CGCCTTCCCA 
CGCCGCCTCG ACTGCACGGA AATCCTGTAC GACCCCTCCA TCATCCAGCA CACGCTGACC 
TGCTTCGGCC GCCGCTTCTT CATGCTGCTG CAAGACGAGC AGGGTGACGT GTACCGCGTC 
TTCTTGGAGG CCGGTGACGT GCAGCGCGCC TACGAGGCGC TTCGGACGCG CGAGCTGGCG 
CAGGCAAACC CAGAGCTGTT CCTGCAGCAG CAGCAGCAGC AGCAGCTAGC GGCGAACGGG 
GGTGTTGCGC CGGCTCCGGT GCTGCCGCCC GTTCCGAACC CGCTGTCGGT GCACTACTTC 
GACACGTTGC CGCCGTCGTC GGCCATGGCA CTCTTCCGGC GCGGCTTTCT GTTCATTGGC 
AATGAGGCAG GCCCGCTGCA TGGGCTGTAC AAAATCAAGA GCAACGGCTA CACGGCGGAT 
CGCGAGTACA TCGTGAAGCG CGTGCGGCGG GAGCGGGCGC TGCCGATGCT GTCGTCAAGC 
AAGGCGGAGA AGGCGGCGAA GGCAGAGGTG GAGGACGGCG CTGTGAAGAA GGAAGGCGAC 
GGTGCCGATC GCAAGCGTCA GCGCGCTGCG GATGCCGCCG GGACTGCCGT CAAGCCAGAG 
CCGCAGGACC CGACGGCGCC GGCGACAAGC AGCGGCAGCG CCGCATCGTC CACGCTGCCA 
CGCATCAAGA CGGAGCAGAC GGATGGCGAC GGAGACAACC TGGCGGCGGC TGCGGCTAGC 
GCTACCACCA TGCGGCCACC GGCCCCCTCC ACAGCCACGG CGCCGGCTTT CAAAACTCCG 
ATGGAGGTGC GTGTCATCAA GGTCTACCAG CCGCACCGCC GTCCTCAGCA CGTCGTCCAG 
CTACAGAGCT TCCCGAACAC GCCGGCCATC ACAAGCTTCA CCTCCCACCT CACCGCCTCC 
ACCTCCACCC CGGCGACGGC GTCGGACGGC GCTGACCACC CCACCGGCTT TGGTGGTGGG 
CCGGCGCCGG CGCCGGAGCA GTCGAGTGAG CAGCCGGAGA TCTACTTCAG CTACGTCGGC 
GGGCGTGGCG GGGAGAGCGT GCTCGTGCAG GCCCGCTACG GCTACGCCGC GAAGTTGGAA 
GGGCGGCAGA TGCTCCCAAC CGTCTTCAGC TTCGTCATCC CTCTGCCCTC TGCGCTGTCC 
ATGCAGTCCC TAGCGCACCA GCAGCAGGCT GCCATCGAAG CAGCCGCGGC TGCAGCCGTC 
ACGCGAAGGG CTGACGAAAA GACGCTCAGA GGCACTGGCG GTGCGGCGCG TCAGCACGCG 
CAGCGCTTGA AGGCGCTTCG CCAGCTCGCC GCGACCGTCA GCGGCGCTCG TGACCAGGTT 
TGCACCGACC GCGTGCTGCT GTGCACGCGC CAGGGCACGA CGGTGTACCG CATCGGTAGC 
ACGGTCGAGC AGGACACCGG CTCGTCTTTC ATCACCGCAG AGCGGACGCT CGCCGCGACG 
ACGCTGCAGT ACGGCTGCGG CTACGTGCAG GTTACCCCGC GTGGAATAAA CGTGCTGAGC 
TGCGTCGACA CCGCCGCGGG CGGTGGCGGT CGACTTGACG CAGCCGGCAG AAGAGTGCAC 
GGCAGCGGCG ATGGCGCAGG GGCGTCATCG GTCGTTCCAG CGAGCTGGAC GCATCCGCAC 
GGCAGCACCG TCCTCGCTGC CGCGGTGACC CCGACGACGG TGCTGCTTTC GTTCCATCAG 
CGCGGCGGCA TCGCCAGCTT CGACTTTGGC CTGGCAGGCA CGGAGCTTCA GCAACGCGAC 
GTCCTTCCCA CCTTCCCGCT GGCCCCCGTT GTGGCCCTGC TGCAGCCACC GGCAAGCGCC 
GTATCAGCAG CCCTCCAGCG CGAACTTCAG GCGCTCTTGT GGAACTCGAG TGCCGCGTCG 
CCAAGCTCGG CAGCGGCCGC TGCGGCCGCC TCGGCCGCCG CTGCGGCGGC TGCGGCTCAG 
ACGGAGCAGC TCGCGGCCAT CGCGACCGTG AACCACGAGG TATACCTCGT CCACCCCCGC 
AAGCTGCGCG AGCCTCTGGA GAAGATCACT TGTCGTCGCA GCAGCAGCCC GGACGCCGGC 
TCATCGTCGT CAGCCGAGGT GACGATCACC TCTCTTCTCC TGACCTATCT CGGCGACACG 
CGAGGCGGCA GTGACGCGGC GGCGGCGCTG ATCGGTGGCG CGGGCCCGCG CAGCGGCAGA 
GGCGGCAACC GTGTGCGTGG CGGCGTGGCC GCCGCCGCGG GGCGACGGCA GCTATTCCTG 
TTCGTCGGCC ATAGTGACGG CATGGTGACG CGGTGCGAGC TGAACCCGTC GTCTGCGAAG 
GTGACCGCGA CGGCGGAGCT GTGGTGTGGC GCCCGCCCGT GCCAGCTCGT CGCAGGCGAT 
GGCGAAACCT ATTGCTACGT CGTCTCGGGC GAGCGGACGT GGCGGTGCGA GATGCAGGAT 
AGGACGGTGA GGATGGCGCC GTACAGCTTT CCAACGCGGC CGACGTCGTT TGCGCGGTTC 
GTGCTGCCGT CGCGGGTCAG CGCTGCCGCC GCGAACGGCA GCGACTCGGC GGCCCCAGGG 
GCGCAGGACA GCGCGAATGA GGAGGACGAG AACGCCCTTG CCGCGGTCGA GTCCATCACC 
CTCGTTCCGC GCGAGCAGCA GCAGGAGGTG GTCATTGCCG TGCAGAACCG CGAGTTGGCT 
CTCTACGTGT CCAACATCGG CGCCGGTGGT GGTGGCGCTG GTGGTGGCGG CGGCGGTGGC 
CCCGGCTCGG GCGGCGTGGA GTACAGCTTC CAGCACCATC CGCTGCCGCT GGCGGGTCGC 
CGGCTCCTGC AGCACCCAAC ACGCCCAGCG TACCTCATTC TCTGCGGTGT CGAGCACCGC 
AGCCATGGCC GGCAGGAGGC AGAGCGGCAC CGCGCCGAGG ATCGAGCCTT CCTCACGGCT 
CGCATGGCGG ACACCACGCA GGCCGAGGAC CGCGGTGCAC TCTACGTCGA GGGTGTATAC 
GGCGGCATCC CCTACACGGC GCCGCAGCGC TCCCTTGGAC GCCCCAACGC GTACCACAGT 
ACGCTGCAGC TCTACCACCG GGATGCCCAG CGGCTGCTGC CGCCCATCCA CTTTGAGCCC 
AACGAAGCGA TCACCTCCGT TGCCGTGGGA AGCTTCTTCA AGGAGTTTGG TCGCGAGCCG 
GTCGTCATCG TCGGCACGGC ATCGTCGTAC ACGCACGGGG CGGGCTGTGG CACGGCCGCG 
TCGTGGCGGT ATGGCTTTCT GCGCACATTC CGGTGCGCCA GCGGCAGCGT TGGCGGTGGT 
GGCGGCGGCG GCGCTGATGT GTTGCTGAGG CTGGAGCTGC TACACAGCAC GTACCTGCGG 
CCGGATGAGA CCGGCGGCAG CGGCAGGGAT CGTCGCTCCG GTGCCAACAG CAGTGCTGGT 
GCCGCTGGCG GCGGAGGCAA CGGCGTGGGT GGGGACGCGA AGCCCGACTA CCCCTCCGCG 
CTGTGCATCT GCGAGCCGGT GGGCATTCTG CTTGTCGGCA TGGGCGCCGC ACAGGGGCTG 
CGCATCTACT CGTGGGGCAA GGAGCACCTC CTGCGCCGCC GCCACCTCAC CAACACGCCT 
GGTCAGCGCA TCACCGCCAT CGACTACGTT TTCGCGTCGC CGCCGGGCAG CAGGAACCTG 
AACGCGCGCA ATGTGTCCTT CTTTGCTGCT GACCTCTACC AGTGCCCGTA CGGGGACGCC 
GAGGGCATGC GGATGGCGCG GGAGAAGCAG CTGCTTATCA TCTGCGGCAC CGTGAACGCC 
TCCGTGTTCA TTGCCGCGCT ACAGCCTGGG CACCTCGCCC CCGCGGCTGC ACATGGTGCG 
GCGGGCGCGA CGATACCCGC CACGCCATCG TTTCTGCTGC AGATCATGAC CGACAGCGTA 
CCGCGGCACA TCACGAGTGT TGTTGCGGTG GACGAGCGCA CCATCGCCGC GTCGGACCGC 
TTCGGGACGG TCGTGTTCTT GCGCATCCCG GAGACCACGC GCACACAATT CGCGCAGCCC 
GTTCATCAGC TGCAGGACGC GGAGCTCATC GCCGAGGAGG CCTACCTGCG CACCAAGCAG 
ACGTTCCGCG AGGTGGCGCG GCACCACGTC GGTGAGCTCG TGACAGCGCT GCATGTGCAG 
CCGTACGACC CCTCGCAAGG CACCGACGCG GCTCTTGCGA CGAAGATTGT GTACTACAGC 
ACCGCACTCG GCAGCATCGG CGCGTACGTG CCACTGCTCT CGGAAGAGGA CGGCGCTCTG 
GCAGCCTACC TACAGCCGCT CCTGCACTCG CACATGCGCC CGCTGCTCGG CCCGCCCCCT 
GCATTGCCGC CGGCGTCGTA CCTGTCGCAC CATGTGGTGG ACGGCGATGT TGCGCAGTTG 
CTGCGCGGCG GCGCCACGAC GCCGTTCTCT GCAGCCGCGA AGGACGACAT TGCCGAGGAG 
CTGGAGCGGC AAGTCAAGAG GGAAGCGGCA CGTCGTAACA TCTTGGGGCT GCCGAAGCGC 
ACTCTGCCGT CTCTAACGGA GCTCATTGCA AAGCAGCGCG CGCTACTCAC CCTACCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001687566.1
CDS	1..5943
Translation	MNHNSVAEGEDGGTGADAAAVVPVNETLLYHQTLIPTQNVTHAIEGSFTLPDANDLVLIRHNVLELWRLYAENANGGVECVCRTPLMSAVYAAVAVPTSASSMAKAGGTGSAASPGGSVAPVLDDATTTAHRSGVHYLAVTSETGYVTLLRYQLDEPPVPTRLSYGDDSDEGNEGVGGNSGASGGATMLVTTSLKGRFIKVSEVCLGRSGARLTVPGARMVVDPAGTALLITALMRTKTLVPITANETWDPVKSVNVGQDDDDDAESDADGDDEDEDGDDDDAGEHQRRGPVTGRELRAARRRRAAKVRRANGAINLSTPIEMQRQTVLYSACALDGYLDHAVFAVLEEEIVEARDAAAAASASNPANAAAAAAEASGGTFLKSSGGPNAVVQRHKHFVLYAYVPSLKQVQRTQLVHVPVTAHRLIAVPAAPYGPGGVLVCTDTELIWYDVSATQQQQQQNSAAAANGRVGVFKCTYAFPRRLDCTEILYDPSIIQHTLTCFGRRFFMLLQDEQGDVYRVFLEAGDVQRAYEALRTRELAQANPELFLQQQQQQQLAANGGVAPAPVLPPVPNPLSVHYFDTLPPSSAMALFRRGFLFIGNEAGPLHGLYKIKSNGYTADREYIVKRVRRERALPMLSSSKAEKAAKAEVEDGAVKKEGDGADRKRQRAADAAGTAVKPEPQDPTAPATSSGSAASSTLPRIKTEQTDGDGDNLAAAAASATTMRPPAPSTATAPAFKTPMEVRVIKVYQPHRRPQHVVQLQSFPNTPAITSFTSHLTASTSTPATASDGADHPTGFGGGPAPAPEQSSEQPEIYFSYVGGRGGESVLVQARYGYAAKLEGRQMLPTVFSFVIPLPSALSMQSLAHQQQAAIEAAAAAAVTRRADEKTLRGTGGAARQHAQRLKALRQLAATVSGARDQVCTDRVLLCTRQGTTVYRIGSTVEQDTGSSFITAERTLAATTLQYGCGYVQVTPRGINVLSCVDTAAGGGGRLDAAGRRVHGSGDGAGASSVVPASWTHPHGSTVLAAAVTPTTVLLSFHQRGGIASFDFGLAGTELQQRDVLPTFPLAPVVALLQPPASAVSAALQRELQALLWNSSAASPSSAAAAAAASAAAAAAAAQTEQLAAIATVNHEVYLVHPRKLREPLEKITCRRSSSPDAGSSSSAEVTITSLLLTYLGDTRGGSDAAAALIGGAGPRSGRGGNRVRGGVAAAAGRRQLFLFVGHSDGMVTRCELNPSSAKVTATAELWCGARPCQLVAGDGETYCYVVSGERTWRCEMQDRTVRMAPYSFPTRPTSFARFVLPSRVSAAAANGSDSAAPGAQDSANEEDENALAAVESITLVPREQQQEVVIAVQNRELALYVSNIGAGGGGAGGGGGGGPGSGGVEYSFQHHPLPLAGRRLLQHPTRPAYLILCGVEHRSHGRQEAERHRAEDRAFLTARMADTTQAEDRGALYVEGVYGGIPYTAPQRSLGRPNAYHSTLQLYHRDAQRLLPPIHFEPNEAITSVAVGSFFKEFGREPVVIVGTASSYTHGAGCGTAASWRYGFLRTFRCASGSVGGGGGGGADVLLRLELLHSTYLRPDETGGSGRDRRSGANSSAGAAGGGGNGVGGDAKPDYPSALCICEPVGILLVGMGAAQGLRIYSWGKEHLLRRRHLTNTPGQRITAIDYVFASPPGSRNLNARNVSFFAADLYQCPYGDAEGMRMAREKQLLIICGTVNASVFIAALQPGHLAPAAAHGAAGATIPATPSFLLQIMTDSVPRHITSVVAVDERTIAASDRFGTVVFLRIPETTRTQFAQPVHQLQDAELIAEEAYLRTKQTFREVARHHVGELVTALHVQPYDPSQGTDAALATKIVYYSTALGSIGAYVPLLSEEDGALAAYLQPLLHSHMRPLLGPPPALPPASYLSHHVVDGDVAQLLRGGATTPFSAAAKDDIAEELERQVKREAARRNILGLPKRTLPSLTELIAKQRALLTLPL

Target ORF information:

RefSeq Version	XM_001687514.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001687514.1

ORF Insert Sequence:

ATGAACCACA ACAGCGTGGC GGAGGGAGAA GATGGCGGCA CCGGTGCAGA CGCCGCCGCC 
GTCGTGCCCG TGAACGAGAC GCTGCTGTAC CACCAGACCT TGATCCCCAC ACAGAATGTC 
ACCCACGCCA TCGAGGGCTC CTTCACGCTG CCGGACGCGA ACGACCTCGT TCTGATCCGT 
CACAACGTAC TCGAGCTGTG GCGACTTTAC GCAGAGAACG CCAACGGCGG CGTAGAGTGT 
GTTTGCCGCA CCCCGTTGAT GTCGGCTGTG TACGCTGCGG TGGCGGTGCC GACAAGCGCC 
TCGTCGATGG CGAAGGCCGG TGGCACGGGC AGTGCCGCGT CGCCGGGCGG AAGTGTTGCC 
CCTGTACTCG ACGACGCGAC GACCACAGCG CACCGCAGTG GGGTACACTA CCTCGCCGTC 
ACGAGTGAGA CGGGCTACGT GACACTGCTG CGGTACCAGC TGGACGAGCC GCCGGTGCCG 
ACGCGGCTGT CCTACGGCGA CGACAGCGAC GAGGGCAACG AAGGTGTTGG TGGGAACAGT 
GGCGCTAGCG GCGGTGCCAC GATGCTCGTG ACCACGTCCT TGAAGGGCCG CTTCATCAAG 
GTCAGCGAGG TGTGCCTGGG CCGGAGCGGG GCGCGGCTGA CCGTCCCTGG TGCCCGCATG 
GTGGTCGACC CCGCCGGCAC GGCGCTCTTG ATCACCGCGC TCATGCGTAC CAAAACGTTG 
GTGCCCATCA CCGCGAATGA GACCTGGGAT CCGGTCAAGA GCGTCAACGT CGGACAAGAC 
GACGACGACG ACGCAGAGAG CGACGCTGAC GGCGATGACG AGGACGAAGA CGGTGACGAC 
GACGATGCTG GCGAGCACCA GCGCCGCGGC CCGGTGACAG GTCGAGAGCT TCGTGCAGCC 
CGGCGCCGTC GGGCGGCCAA AGTGCGACGG GCCAACGGCG CCATCAACCT CAGCACGCCA 
ATCGAGATGC AGCGCCAGAC CGTCCTGTAC TCCGCCTGCG CCCTTGATGG CTACCTCGAC 
CATGCTGTGT TCGCCGTGCT CGAGGAGGAG ATCGTCGAAG CACGCGACGC CGCCGCCGCG 
GCGTCCGCCA GTAACCCCGC GAACGCCGCG GCAGCCGCAG CCGAGGCGAG CGGTGGCACC 
TTCCTCAAGA GCAGCGGAGG CCCGAACGCG GTGGTTCAGC GCCACAAGCA CTTCGTGCTC 
TACGCCTACG TGCCGAGCCT GAAGCAGGTG CAGCGGACGC AGCTTGTGCA CGTTCCAGTG 
ACGGCGCACC GTCTCATCGC TGTCCCGGCC GCACCGTACG GCCCAGGCGG CGTTCTTGTC 
TGCACAGACA CGGAGCTGAT CTGGTACGAT GTGTCCGCCA CGCAACAGCA GCAGCAGCAG 
AACTCCGCTG CGGCGGCGAA TGGCCGCGTC GGCGTCTTCA AGTGCACCTA CGCCTTCCCA 
CGCCGCCTCG ACTGCACGGA AATCCTGTAC GACCCCTCCA TCATCCAGCA CACGCTGACC 
TGCTTCGGCC GCCGCTTCTT CATGCTGCTG CAAGACGAGC AGGGTGACGT GTACCGCGTC 
TTCTTGGAGG CCGGTGACGT GCAGCGCGCC TACGAGGCGC TTCGGACGCG CGAGCTGGCG 
CAGGCAAACC CAGAGCTGTT CCTGCAGCAG CAGCAGCAGC AGCAGCTAGC GGCGAACGGG 
GGTGTTGCGC CGGCTCCGGT GCTGCCGCCC GTTCCGAACC CGCTGTCGGT GCACTACTTC 
GACACGTTGC CGCCGTCGTC GGCCATGGCA CTCTTCCGGC GCGGCTTTCT GTTCATTGGC 
AATGAGGCAG GCCCGCTGCA TGGGCTGTAC AAAATCAAGA GCAACGGCTA CACGGCGGAT 
CGCGAGTACA TCGTGAAGCG CGTGCGGCGG GAGCGGGCGC TGCCGATGCT GTCGTCAAGC 
AAGGCGGAGA AGGCGGCGAA GGCAGAGGTG GAGGACGGCG CTGTGAAGAA GGAAGGCGAC 
GGTGCCGATC GCAAGCGTCA GCGCGCTGCG GATGCCGCCG GGACTGCCGT CAAGCCAGAG 
CCGCAGGACC CGACGGCGCC GGCGACAAGC AGCGGCAGCG CCGCATCGTC CACGCTGCCA 
CGCATCAAGA CGGAGCAGAC GGATGGCGAC GGAGACAACC TGGCGGCGGC TGCGGCTAGC 
GCTACCACCA TGCGGCCACC GGCCCCCTCC ACAGCCACGG CGCCGGCTTT CAAAACTCCG 
ATGGAGGTGC GTGTCATCAA GGTCTACCAG CCGCACCGCC GTCCTCAGCA CGTCGTCCAG 
CTACAGAGCT TCCCGAACAC GCCGGCCATC ACAAGCTTCA CCTCCCACCT CACCGCCTCC 
ACCTCCACCC CGGCGACGGC GTCGGACGGC GCTGACCACC CCACCGGCTT TGGTGGTGGG 
CCGGCGCCGG CGCCGGAGCA GTCGAGTGAG CAGCCGGAGA TCTACTTCAG CTACGTCGGC 
GGGCGTGGCG GGGAGAGCGT GCTCGTGCAG GCCCGCTACG GCTACGCCGC GAAGTTGGAA 
GGGCGGCAGA TGCTCCCAAC CGTCTTCAGC TTCGTCATCC CTCTGCCCTC TGCGCTGTCC 
ATGCAGTCCC TAGCGCACCA GCAGCAGGCT GCCATCGAAG CAGCCGCGGC TGCAGCCGTC 
ACGCGAAGGG CTGACGAAAA GACGCTCAGA GGCACTGGCG GTGCGGCGCG TCAGCACGCG 
CAGCGCTTGA AGGCGCTTCG CCAGCTCGCC GCGACCGTCA GCGGCGCTCG TGACCAGGTT 
TGCACCGACC GCGTGCTGCT GTGCACGCGC CAGGGCACGA CGGTGTACCG CATCGGTAGC 
ACGGTCGAGC AGGACACCGG CTCGTCTTTC ATCACCGCAG AGCGGACGCT CGCCGCGACG 
ACGCTGCAGT ACGGCTGCGG CTACGTGCAG GTTACCCCGC GTGGAATAAA CGTGCTGAGC 
TGCGTCGACA CCGCCGCGGG CGGTGGCGGT CGACTTGACG CAGCCGGCAG AAGAGTGCAC 
GGCAGCGGCG ATGGCGCAGG GGCGTCATCG GTCGTTCCAG CGAGCTGGAC GCATCCGCAC 
GGCAGCACCG TCCTCGCTGC CGCGGTGACC CCGACGACGG TGCTGCTTTC GTTCCATCAG 
CGCGGCGGCA TCGCCAGCTT CGACTTTGGC CTGGCAGGCA CGGAGCTTCA GCAACGCGAC 
GTCCTTCCCA CCTTCCCGCT GGCCCCCGTT GTGGCCCTGC TGCAGCCACC GGCAAGCGCC 
GTATCAGCAG CCCTCCAGCG CGAACTTCAG GCGCTCTTGT GGAACTCGAG TGCCGCGTCG 
CCAAGCTCGG CAGCGGCCGC TGCGGCCGCC TCGGCCGCCG CTGCGGCGGC TGCGGCTCAG 
ACGGAGCAGC TCGCGGCCAT CGCGACCGTG AACCACGAGG TATACCTCGT CCACCCCCGC 
AAGCTGCGCG AGCCTCTGGA GAAGATCACT TGTCGTCGCA GCAGCAGCCC GGACGCCGGC 
TCATCGTCGT CAGCCGAGGT GACGATCACC TCTCTTCTCC TGACCTATCT CGGCGACACG 
CGAGGCGGCA GTGACGCGGC GGCGGCGCTG ATCGGTGGCG CGGGCCCGCG CAGCGGCAGA 
GGCGGCAACC GTGTGCGTGG CGGCGTGGCC GCCGCCGCGG GGCGACGGCA GCTATTCCTG 
TTCGTCGGCC ATAGTGACGG CATGGTGACG CGGTGCGAGC TGAACCCGTC GTCTGCGAAG 
GTGACCGCGA CGGCGGAGCT GTGGTGTGGC GCCCGCCCGT GCCAGCTCGT CGCAGGCGAT 
GGCGAAACCT ATTGCTACGT CGTCTCGGGC GAGCGGACGT GGCGGTGCGA GATGCAGGAT 
AGGACGGTGA GGATGGCGCC GTACAGCTTT CCAACGCGGC CGACGTCGTT TGCGCGGTTC 
GTGCTGCCGT CGCGGGTCAG CGCTGCCGCC GCGAACGGCA GCGACTCGGC GGCCCCAGGG 
GCGCAGGACA GCGCGAATGA GGAGGACGAG AACGCCCTTG CCGCGGTCGA GTCCATCACC 
CTCGTTCCGC GCGAGCAGCA GCAGGAGGTG GTCATTGCCG TGCAGAACCG CGAGTTGGCT 
CTCTACGTGT CCAACATCGG CGCCGGTGGT GGTGGCGCTG GTGGTGGCGG CGGCGGTGGC 
CCCGGCTCGG GCGGCGTGGA GTACAGCTTC CAGCACCATC CGCTGCCGCT GGCGGGTCGC 
CGGCTCCTGC AGCACCCAAC ACGCCCAGCG TACCTCATTC TCTGCGGTGT CGAGCACCGC 
AGCCATGGCC GGCAGGAGGC AGAGCGGCAC CGCGCCGAGG ATCGAGCCTT CCTCACGGCT 
CGCATGGCGG ACACCACGCA GGCCGAGGAC CGCGGTGCAC TCTACGTCGA GGGTGTATAC 
GGCGGCATCC CCTACACGGC GCCGCAGCGC TCCCTTGGAC GCCCCAACGC GTACCACAGT 
ACGCTGCAGC TCTACCACCG GGATGCCCAG CGGCTGCTGC CGCCCATCCA CTTTGAGCCC 
AACGAAGCGA TCACCTCCGT TGCCGTGGGA AGCTTCTTCA AGGAGTTTGG TCGCGAGCCG 
GTCGTCATCG TCGGCACGGC ATCGTCGTAC ACGCACGGGG CGGGCTGTGG CACGGCCGCG 
TCGTGGCGGT ATGGCTTTCT GCGCACATTC CGGTGCGCCA GCGGCAGCGT TGGCGGTGGT 
GGCGGCGGCG GCGCTGATGT GTTGCTGAGG CTGGAGCTGC TACACAGCAC GTACCTGCGG 
CCGGATGAGA CCGGCGGCAG CGGCAGGGAT CGTCGCTCCG GTGCCAACAG CAGTGCTGGT 
GCCGCTGGCG GCGGAGGCAA CGGCGTGGGT GGGGACGCGA AGCCCGACTA CCCCTCCGCG 
CTGTGCATCT GCGAGCCGGT GGGCATTCTG CTTGTCGGCA TGGGCGCCGC ACAGGGGCTG 
CGCATCTACT CGTGGGGCAA GGAGCACCTC CTGCGCCGCC GCCACCTCAC CAACACGCCT 
GGTCAGCGCA TCACCGCCAT CGACTACGTT TTCGCGTCGC CGCCGGGCAG CAGGAACCTG 
AACGCGCGCA ATGTGTCCTT CTTTGCTGCT GACCTCTACC AGTGCCCGTA CGGGGACGCC 
GAGGGCATGC GGATGGCGCG GGAGAAGCAG CTGCTTATCA TCTGCGGCAC CGTGAACGCC 
TCCGTGTTCA TTGCCGCGCT ACAGCCTGGG CACCTCGCCC CCGCGGCTGC ACATGGTGCG 
GCGGGCGCGA CGATACCCGC CACGCCATCG TTTCTGCTGC AGATCATGAC CGACAGCGTA 
CCGCGGCACA TCACGAGTGT TGTTGCGGTG GACGAGCGCA CCATCGCCGC GTCGGACCGC 
TTCGGGACGG TCGTGTTCTT GCGCATCCCG GAGACCACGC GCACACAATT CGCGCAGCCC 
GTTCATCAGC TGCAGGACGC GGAGCTCATC GCCGAGGAGG CCTACCTGCG CACCAAGCAG 
ACGTTCCGCG AGGTGGCGCG GCACCACGTC GGTGAGCTCG TGACAGCGCT GCATGTGCAG 
CCGTACGACC CCTCGCAAGG CACCGACGCG GCTCTTGCGA CGAAGATTGT GTACTACAGC 
ACCGCACTCG GCAGCATCGG CGCGTACGTG CCACTGCTCT CGGAAGAGGA CGGCGCTCTG 
GCAGCCTACC TACAGCCGCT CCTGCACTCG CACATGCGCC CGCTGCTCGG CCCGCCCCCT 
GCATTGCCGC CGGCGTCGTA CCTGTCGCAC CATGTGGTGG ACGGCGATGT TGCGCAGTTG 
CTGCGCGGCG GCGCCACGAC GCCGTTCTCT GCAGCCGCGA AGGACGACAT TGCCGAGGAG 
CTGGAGCGGC AAGTCAAGAG GGAAGCGGCA CGTCGTAACA TCTTGGGGCT GCCGAAGCGC 
ACTCTGCCGT CTCTAACGGA GCTCATTGCA AAGCAGCGCG CGCTACTCAC CCTACCGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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LMJF_05_0890 cDNA ORF clone, Leishmania major strain Friedlin

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