ALS2 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALS2
Entrez Gene ID	100019560
Full Name	ALS2, alsin Rho guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016425187.1	XP_016280673.1	alsin isoform X1
XM_016425188.1	XP_016280674.1	alsin isoform X1
XM_016425189.1	XP_016280675.1	alsin isoform X1
XM_016425191.1	XP_016280677.1	alsin isoform X1
XM_016425193.1	XP_016280679.1	alsin isoform X2
XM_016425190.1	XP_016280676.1	alsin isoform X1

Pathways from BioSystems
Gene Ontology

The following ALS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc44704	XM_016425190.1 Latest version!	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc44704	XM_016425187.1 Latest version!	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc44704	XM_016425188.1 Latest version!	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc44704	XM_016425191.1 Latest version!	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc44704	XM_016425189.1 Latest version!	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc44705	XM_016425193.1 Latest version!	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALS2 ( XM_016425187.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016280673.1 Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc44704
Clone ID Related Accession (Same CDS sequence)	XM_016425187.1 , XM_016425188.1 , XM_016425189.1 , XM_016425191.1 , XM_016425190.1
Accession Version	XM_016425187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008807.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016280673.1
CDS	694..5670
Translation	MDSPERSSAEAEGAKERGLVHVWQAGFCSITPERLPGWGGKTVLQAALGVSHGVLLTEGGEVYSFGNLPWRSEPEEICPKSPLLENALVGQHVITVATGSFHSGAVTENGMVYMWGQNSAGQCAIANQHHVPEPTPVSISDSETSPLLSVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGDSHSLALVQCLPSQDVKPVPERCNHCSQLLITMTDKEDHVIISDSHCCPLGVALSETQAESHDAITPSTGESLESKNLSKSQGGEKKTLRENGLSVASEVNIENMQPVNAEATHPEQEITEATGIPILGNVPLFPDNHVEKACLQKLSDDSLQGHPETGGEVEVAQSLTEEPNPSLPSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSIAPCESGLQSGNASLGPEGLKDSREEQVKLESMQGKKSSSLVDIREEESEGVSRRLSLPGLLSQVSPRLLRKAARAKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIQLAAGGHHSLALTAKSQVYSWGSNTFGQLGHSDSPTTIPRLAKVCDENGIWSIAAGQDYSLFLADTDDFQPGLYYSGRQEPAESDHDICTKTASLLGSCSKLGYISSVTAGKNSCLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTTTTVQLLQDVASRFSKLCYLIGQHGASLSSFLHGIKEAKSLAILKHASLFLDSYTEYCTSITNFVVMGGFQLLAKPATDFLNKNQELLQDLSEVNEENIQLVELLNTLFFLPIRRLHNYARVLLKLATCFEVTSPEYHKLQDSSSCYETLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLICESSNRALSLQHAGRFSVNWFILFNDSLVHAQFSTHHIFPLSTLWVEPISEEVGSVNGLKITTPEEQFILVSSTPQEKTKWLRAISQAVDQALRGMSDFPPYGSGSSVPRQEPPISRSARYTFYKDPRLKDAIYDGRWLSGKPHGRGTLKWTDGKMYSGMFRNGLEDGYGEYKVPNKALNKDDHYVGYWKEGKMCGQGVYSYANGEVYEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKSGYGVFDDITRGEKYMGMWQDDMCQGNGVVVTQFGLYYEGTFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTMPNGDYIEGLFNGEWGSGIKITGTYFKPSLYESEKDRPKILRKLGNLAVPADEKWKAVFEECWHQLGCENPGQGELWKAWDNIAVALTTNRRQHRDSPEILSRSHTQTLESLEFIPQHVGGAFTVEKYDEIKKYLIKACDTPLHPLCRLIETLVAVYRMTYVGVGANRRLLQEAVKEIQSYLKRIFQLVRFLFPDLPEEGSTIPLSVPLPQERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALENDREEDIYWECVLRLNKQPDTALLSFLGVQRKFWPVTLSVFGDTKKVLPTTKDSCFASAVECLQQISTTFTPSDKLKVIHQTFEEISQSVLATLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKVSVFHLFNNAD

Target ORF information:

RefSeq Version	XM_016425187.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425187.1

ORF Insert Sequence:

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_016425188.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016280674.1 Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMc44704
Clone ID Related Accession (Same CDS sequence)	XM_016425187.1 , XM_016425188.1 , XM_016425189.1 , XM_016425191.1 , XM_016425190.1
Accession Version	XM_016425188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008807.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016280674.1
CDS	774..5750
Translation	MDSPERSSAEAEGAKERGLVHVWQAGFCSITPERLPGWGGKTVLQAALGVSHGVLLTEGGEVYSFGNLPWRSEPEEICPKSPLLENALVGQHVITVATGSFHSGAVTENGMVYMWGQNSAGQCAIANQHHVPEPTPVSISDSETSPLLSVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGDSHSLALVQCLPSQDVKPVPERCNHCSQLLITMTDKEDHVIISDSHCCPLGVALSETQAESHDAITPSTGESLESKNLSKSQGGEKKTLRENGLSVASEVNIENMQPVNAEATHPEQEITEATGIPILGNVPLFPDNHVEKACLQKLSDDSLQGHPETGGEVEVAQSLTEEPNPSLPSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSIAPCESGLQSGNASLGPEGLKDSREEQVKLESMQGKKSSSLVDIREEESEGVSRRLSLPGLLSQVSPRLLRKAARAKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIQLAAGGHHSLALTAKSQVYSWGSNTFGQLGHSDSPTTIPRLAKVCDENGIWSIAAGQDYSLFLADTDDFQPGLYYSGRQEPAESDHDICTKTASLLGSCSKLGYISSVTAGKNSCLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTTTTVQLLQDVASRFSKLCYLIGQHGASLSSFLHGIKEAKSLAILKHASLFLDSYTEYCTSITNFVVMGGFQLLAKPATDFLNKNQELLQDLSEVNEENIQLVELLNTLFFLPIRRLHNYARVLLKLATCFEVTSPEYHKLQDSSSCYETLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLICESSNRALSLQHAGRFSVNWFILFNDSLVHAQFSTHHIFPLSTLWVEPISEEVGSVNGLKITTPEEQFILVSSTPQEKTKWLRAISQAVDQALRGMSDFPPYGSGSSVPRQEPPISRSARYTFYKDPRLKDAIYDGRWLSGKPHGRGTLKWTDGKMYSGMFRNGLEDGYGEYKVPNKALNKDDHYVGYWKEGKMCGQGVYSYANGEVYEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKSGYGVFDDITRGEKYMGMWQDDMCQGNGVVVTQFGLYYEGTFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTMPNGDYIEGLFNGEWGSGIKITGTYFKPSLYESEKDRPKILRKLGNLAVPADEKWKAVFEECWHQLGCENPGQGELWKAWDNIAVALTTNRRQHRDSPEILSRSHTQTLESLEFIPQHVGGAFTVEKYDEIKKYLIKACDTPLHPLCRLIETLVAVYRMTYVGVGANRRLLQEAVKEIQSYLKRIFQLVRFLFPDLPEEGSTIPLSVPLPQERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALENDREEDIYWECVLRLNKQPDTALLSFLGVQRKFWPVTLSVFGDTKKVLPTTKDSCFASAVECLQQISTTFTPSDKLKVIHQTFEEISQSVLATLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKVSVFHLFNNAD

Target ORF information:

RefSeq Version	XM_016425188.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425188.1

ORF Insert Sequence:

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_016425189.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016280675.1 Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

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CloneID	OMc44704
Clone ID Related Accession (Same CDS sequence)	XM_016425187.1 , XM_016425188.1 , XM_016425189.1 , XM_016425191.1 , XM_016425190.1
Accession Version	XM_016425189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008807.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016280675.1
CDS	396..5372
Translation	MDSPERSSAEAEGAKERGLVHVWQAGFCSITPERLPGWGGKTVLQAALGVSHGVLLTEGGEVYSFGNLPWRSEPEEICPKSPLLENALVGQHVITVATGSFHSGAVTENGMVYMWGQNSAGQCAIANQHHVPEPTPVSISDSETSPLLSVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGDSHSLALVQCLPSQDVKPVPERCNHCSQLLITMTDKEDHVIISDSHCCPLGVALSETQAESHDAITPSTGESLESKNLSKSQGGEKKTLRENGLSVASEVNIENMQPVNAEATHPEQEITEATGIPILGNVPLFPDNHVEKACLQKLSDDSLQGHPETGGEVEVAQSLTEEPNPSLPSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSIAPCESGLQSGNASLGPEGLKDSREEQVKLESMQGKKSSSLVDIREEESEGVSRRLSLPGLLSQVSPRLLRKAARAKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIQLAAGGHHSLALTAKSQVYSWGSNTFGQLGHSDSPTTIPRLAKVCDENGIWSIAAGQDYSLFLADTDDFQPGLYYSGRQEPAESDHDICTKTASLLGSCSKLGYISSVTAGKNSCLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTTTTVQLLQDVASRFSKLCYLIGQHGASLSSFLHGIKEAKSLAILKHASLFLDSYTEYCTSITNFVVMGGFQLLAKPATDFLNKNQELLQDLSEVNEENIQLVELLNTLFFLPIRRLHNYARVLLKLATCFEVTSPEYHKLQDSSSCYETLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLICESSNRALSLQHAGRFSVNWFILFNDSLVHAQFSTHHIFPLSTLWVEPISEEVGSVNGLKITTPEEQFILVSSTPQEKTKWLRAISQAVDQALRGMSDFPPYGSGSSVPRQEPPISRSARYTFYKDPRLKDAIYDGRWLSGKPHGRGTLKWTDGKMYSGMFRNGLEDGYGEYKVPNKALNKDDHYVGYWKEGKMCGQGVYSYANGEVYEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKSGYGVFDDITRGEKYMGMWQDDMCQGNGVVVTQFGLYYEGTFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTMPNGDYIEGLFNGEWGSGIKITGTYFKPSLYESEKDRPKILRKLGNLAVPADEKWKAVFEECWHQLGCENPGQGELWKAWDNIAVALTTNRRQHRDSPEILSRSHTQTLESLEFIPQHVGGAFTVEKYDEIKKYLIKACDTPLHPLCRLIETLVAVYRMTYVGVGANRRLLQEAVKEIQSYLKRIFQLVRFLFPDLPEEGSTIPLSVPLPQERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALENDREEDIYWECVLRLNKQPDTALLSFLGVQRKFWPVTLSVFGDTKKVLPTTKDSCFASAVECLQQISTTFTPSDKLKVIHQTFEEISQSVLATLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKVSVFHLFNNAD

Target ORF information:

RefSeq Version	XM_016425189.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425189.1

ORF Insert Sequence:

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_016425191.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016280677.1 Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X5, mRNA.

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CloneID	OMc44704
Clone ID Related Accession (Same CDS sequence)	XM_016425187.1 , XM_016425188.1 , XM_016425189.1 , XM_016425191.1 , XM_016425190.1
Accession Version	XM_016425191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008807.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016280677.1
CDS	134..5110
Translation	MDSPERSSAEAEGAKERGLVHVWQAGFCSITPERLPGWGGKTVLQAALGVSHGVLLTEGGEVYSFGNLPWRSEPEEICPKSPLLENALVGQHVITVATGSFHSGAVTENGMVYMWGQNSAGQCAIANQHHVPEPTPVSISDSETSPLLSVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGDSHSLALVQCLPSQDVKPVPERCNHCSQLLITMTDKEDHVIISDSHCCPLGVALSETQAESHDAITPSTGESLESKNLSKSQGGEKKTLRENGLSVASEVNIENMQPVNAEATHPEQEITEATGIPILGNVPLFPDNHVEKACLQKLSDDSLQGHPETGGEVEVAQSLTEEPNPSLPSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSIAPCESGLQSGNASLGPEGLKDSREEQVKLESMQGKKSSSLVDIREEESEGVSRRLSLPGLLSQVSPRLLRKAARAKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIQLAAGGHHSLALTAKSQVYSWGSNTFGQLGHSDSPTTIPRLAKVCDENGIWSIAAGQDYSLFLADTDDFQPGLYYSGRQEPAESDHDICTKTASLLGSCSKLGYISSVTAGKNSCLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTTTTVQLLQDVASRFSKLCYLIGQHGASLSSFLHGIKEAKSLAILKHASLFLDSYTEYCTSITNFVVMGGFQLLAKPATDFLNKNQELLQDLSEVNEENIQLVELLNTLFFLPIRRLHNYARVLLKLATCFEVTSPEYHKLQDSSSCYETLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLICESSNRALSLQHAGRFSVNWFILFNDSLVHAQFSTHHIFPLSTLWVEPISEEVGSVNGLKITTPEEQFILVSSTPQEKTKWLRAISQAVDQALRGMSDFPPYGSGSSVPRQEPPISRSARYTFYKDPRLKDAIYDGRWLSGKPHGRGTLKWTDGKMYSGMFRNGLEDGYGEYKVPNKALNKDDHYVGYWKEGKMCGQGVYSYANGEVYEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKSGYGVFDDITRGEKYMGMWQDDMCQGNGVVVTQFGLYYEGTFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTMPNGDYIEGLFNGEWGSGIKITGTYFKPSLYESEKDRPKILRKLGNLAVPADEKWKAVFEECWHQLGCENPGQGELWKAWDNIAVALTTNRRQHRDSPEILSRSHTQTLESLEFIPQHVGGAFTVEKYDEIKKYLIKACDTPLHPLCRLIETLVAVYRMTYVGVGANRRLLQEAVKEIQSYLKRIFQLVRFLFPDLPEEGSTIPLSVPLPQERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALENDREEDIYWECVLRLNKQPDTALLSFLGVQRKFWPVTLSVFGDTKKVLPTTKDSCFASAVECLQQISTTFTPSDKLKVIHQTFEEISQSVLATLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKVSVFHLFNNAD

Target ORF information:

RefSeq Version	XM_016425191.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425191.1

ORF Insert Sequence:

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_016425190.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016280676.1 Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.

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CloneID	OMc44704
Clone ID Related Accession (Same CDS sequence)	XM_016425187.1 , XM_016425188.1 , XM_016425189.1 , XM_016425191.1 , XM_016425190.1
Accession Version	XM_016425190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008807.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016280676.1
CDS	1497..6473
Translation	MDSPERSSAEAEGAKERGLVHVWQAGFCSITPERLPGWGGKTVLQAALGVSHGVLLTEGGEVYSFGNLPWRSEPEEICPKSPLLENALVGQHVITVATGSFHSGAVTENGMVYMWGQNSAGQCAIANQHHVPEPTPVSISDSETSPLLSVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGDSHSLALVQCLPSQDVKPVPERCNHCSQLLITMTDKEDHVIISDSHCCPLGVALSETQAESHDAITPSTGESLESKNLSKSQGGEKKTLRENGLSVASEVNIENMQPVNAEATHPEQEITEATGIPILGNVPLFPDNHVEKACLQKLSDDSLQGHPETGGEVEVAQSLTEEPNPSLPSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSIAPCESGLQSGNASLGPEGLKDSREEQVKLESMQGKKSSSLVDIREEESEGVSRRLSLPGLLSQVSPRLLRKAARAKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIQLAAGGHHSLALTAKSQVYSWGSNTFGQLGHSDSPTTIPRLAKVCDENGIWSIAAGQDYSLFLADTDDFQPGLYYSGRQEPAESDHDICTKTASLLGSCSKLGYISSVTAGKNSCLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTTTTVQLLQDVASRFSKLCYLIGQHGASLSSFLHGIKEAKSLAILKHASLFLDSYTEYCTSITNFVVMGGFQLLAKPATDFLNKNQELLQDLSEVNEENIQLVELLNTLFFLPIRRLHNYARVLLKLATCFEVTSPEYHKLQDSSSCYETLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLICESSNRALSLQHAGRFSVNWFILFNDSLVHAQFSTHHIFPLSTLWVEPISEEVGSVNGLKITTPEEQFILVSSTPQEKTKWLRAISQAVDQALRGMSDFPPYGSGSSVPRQEPPISRSARYTFYKDPRLKDAIYDGRWLSGKPHGRGTLKWTDGKMYSGMFRNGLEDGYGEYKVPNKALNKDDHYVGYWKEGKMCGQGVYSYANGEVYEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKSGYGVFDDITRGEKYMGMWQDDMCQGNGVVVTQFGLYYEGTFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTMPNGDYIEGLFNGEWGSGIKITGTYFKPSLYESEKDRPKILRKLGNLAVPADEKWKAVFEECWHQLGCENPGQGELWKAWDNIAVALTTNRRQHRDSPEILSRSHTQTLESLEFIPQHVGGAFTVEKYDEIKKYLIKACDTPLHPLCRLIETLVAVYRMTYVGVGANRRLLQEAVKEIQSYLKRIFQLVRFLFPDLPEEGSTIPLSVPLPQERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALENDREEDIYWECVLRLNKQPDTALLSFLGVQRKFWPVTLSVFGDTKKVLPTTKDSCFASAVECLQQISTTFTPSDKLKVIHQTFEEISQSVLATLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKVSVFHLFNNAD

Target ORF information:

RefSeq Version	XM_016425190.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425190.1

ORF Insert Sequence:

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGGTAG CACAGTCTCT TACTGAAGAA 
CCAAATCCCA GTCTCCCCAG CCCACCAACC ACAAGTACAT CTGCTCTAAA CAGCTTAGTG 
GTTTCCTGTG CCTCCGCGGT TGGTGTGAGA GTAGCGGCTA CATATGAAGC TGGAGCCCTG 
TCTCTTAAGA AAGTAATGAA CTTTTATAGT ATAGCTCCTT GTGAAAGTGG ACTTCAGTCA 
GGCAATGCTT CCTTGGGCCC AGAAGGATTA AAGGATAGCC GAGAAGAGCA GGTTAAACTA 
GAATCAATGC AAGGAAAAAA GAGTTCAAGT CTTGTGGATA TTAGAGAAGA GGAATCTGAA 
GGAGTTAGCA GAAGGCTTTC TCTTCCTGGA TTATTATCAC AAGTTTCTCC CAGGCTTCTG 
AGAAAAGCTG CCCGGGCAAA AACACGTACA GTGGTATTGA CACCTACATA TAGTGGGGAA 
GCAGATGCCC TCCTCCCTTC TTTAAGGACT GAAGTGTGGA CTTGGGGAAA AGGAAAAGAA 
GGACAGCTAG GACATGGTGA TGTTTTGCCT AGACTTCAGC CATTATGTGT AAAATGTTTA 
GATGGTAAAG AAGTAATTCA ACTGGCAGCT GGTGGTCATC ATTCCCTTGC TCTCACTGCT 
AAATCCCAGG TTTACTCATG GGGTAGTAAT ACTTTTGGTC AACTTGGTCA TTCAGATTCT 
CCAACTACAA TTCCTCGTCT GGCAAAGGTG TGTGATGAAA ATGGAATCTG GAGTATAGCT 
GCTGGGCAGG ATTATTCCCT TTTTTTGGCA GATACAGATG ATTTCCAGCC TGGATTGTAT 
TACAGTGGTC GGCAAGAACC TGCTGAAAGT GATCATGATA TTTGTACTAA GACTGCATCA 
CTGCTGGGAT CCTGTAGTAA ACTTGGGTAC ATAAGCAGTG TGACAGCAGG AAAAAACAGC 
TGCCTAGCTT TGGTGGACAA AAATATTATG GGATATATTG CCAGTCTTCA TGAGCTGGCC 
ACTACAGAAA GACGGTTCTA TTCAAAACTG AGTGATATTA AATCCCAGAT TCTCAGACCC 
CTCCTAGGTT TAGAAAATTT GGGTACCACC ACTACAGTCC AGTTGTTGCA GGATGTGGCA 
AGCAGATTCA GCAAGCTGTG TTACCTGATT GGTCAGCATG GAGCCTCTCT GAGCAGCTTT 
CTTCATGGAA TTAAGGAAGC CAAGAGTTTG GCCATCTTGA AGCATGCAAG TCTCTTCTTG 
GATAGTTATA CAGAGTATTG CACATCTATT ACAAATTTTG TGGTTATGGG AGGATTCCAA 
CTTCTGGCCA AGCCAGCTAC TGACTTCTTA AATAAAAACC AAGAGTTATT ACAAGACTTA 
TCAGAGGTGA ATGAGGAAAA TATCCAATTG GTGGAACTGC TAAACACTTT GTTTTTTTTG 
CCAATCAGAC GTCTTCATAA TTATGCTCGA GTTTTGCTAA AGCTTGCTAC TTGTTTTGAA 
GTGACATCTC CAGAGTATCA TAAACTGCAG GATTCCAGCT CTTGTTATGA GACTCTTGCT 
CTCCATCTCG GCAGGAAAAG GAAGGAAGCA GAATACACAC TGGGCTTCTG GAAGACCTTT 
CCTGGGAAAA TGACGGATTC CTTGAGGAAA CCAGAACGTA GACTAATCTG TGAGAGTAGT 
AATCGAGCAT TATCTCTACA ACATGCTGGT AGATTCTCTG TGAATTGGTT CATTCTCTTT 
AATGATTCTT TGGTCCATGC CCAGTTCTCC ACACATCATA TTTTCCCCTT ATCTACTCTG 
TGGGTTGAGC CTATTTCAGA AGAAGTTGGT AGTGTGAATG GCTTAAAGAT AACAACACCT 
GAAGAACAAT TTATTCTTGT TTCATCAACA CCACAGGAAA AAACTAAATG GTTACGGGCT 
ATAAGCCAAG CTGTGGATCA AGCTCTCCGT GGTATGTCTG ATTTTCCCCC ATATGGAAGT 
GGAAGCAGTG TTCCCAGGCA AGAGCCTCCT ATTTCACGAA GTGCCAGATA CACTTTCTAC 
AAGGACCCTC GACTAAAGGA TGCCATTTAT GATGGACGAT GGCTTTCAGG GAAACCCCAT 
GGCAGAGGGA CTCTGAAATG GACTGATGGA AAGATGTATT CTGGTATGTT CAGAAATGGT 
CTGGAAGATG GGTATGGGGA ATACAAAGTC CCAAATAAAG CACTGAACAA GGATGACCAT 
TATGTGGGAT ATTGGAAAGA AGGAAAAATG TGTGGTCAAG GAGTCTACAG CTATGCCAAT 
GGAGAGGTAT ATGAAGGCTG CTTTCAGGAT AACATGCGTC ACGGTCATGG CCTTCTACGC 
AGTGGTAAAC TGACTTCTTC TTCTCCCAGT ATGTTCATTG GCCAATGGGT GATGGATAAA 
AAGTCTGGAT ATGGTGTATT TGATGATATC ACAAGGGGAG AAAAATATAT GGGAATGTGG 
CAAGATGATA TGTGTCAGGG GAATGGTGTG GTAGTTACCC AGTTTGGATT ATACTATGAA 
GGAACATTTA GCCTTAATAA AATGATGGGA ACTGGTATTT TACTCTCAGA AGATGATACT 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACACTTAATG GAAAGGGAAC ACTGACTATG 
CCAAATGGTG ATTACATTGA AGGTCTTTTC AATGGGGAAT GGGGATCTGG GATAAAAATT 
ACTGGAACAT ACTTCAAACC CAGTTTGTAC GAGAGTGAGA AAGACCGCCC CAAAATTCTT 
AGGAAACTAG GAAACCTGGC AGTGCCTGCT GATGAGAAAT GGAAAGCAGT ATTTGAAGAA 
TGCTGGCACC AGTTAGGCTG TGAGAACCCA GGCCAGGGAG AACTCTGGAA AGCATGGGAC 
AACATCGCTG TAGCCCTAAC CACCAATCGT CGCCAGCACC GAGATAGTCC AGAGATATTA 
AGTCGTTCAC ATACCCAGAC ATTGGAAAGT TTGGAATTCA TTCCACAGCA TGTTGGTGGT 
GCCTTCACTG TCGAGAAATA TGATGAAATA AAGAAATACT TAATAAAGGC TTGTGACACT 
CCTCTGCACC CTTTGTGTCG TCTCATAGAA ACCTTGGTTG CAGTGTATAG GATGACCTAT 
GTGGGAGTGG GAGCTAATCG GCGATTATTG CAAGAAGCTG TGAAAGAGAT TCAGTCCTAC 
CTTAAGCGTA TTTTCCAGCT GGTCAGGTTC CTATTTCCTG ATCTTCCTGA AGAGGGCAGC 
ACAATTCCAT TGTCTGTTCC TCTACCCCAG GAAAGGAAAT CTTTCTGCAC AGGAAAGTCT 
GATTCTAGGT CAGAATCTCC AGAACCAGGC TATGTGGTCA CGAGTTCTGG GTTACTGCTG 
CCTGTACTGT TACCTCGACT CTACCCACCA CTCTTTATGC TGTATGCCTT GGAGAATGAT 
AGAGAGGAAG ATATTTATTG GGAGTGTGTT CTTCGACTCA ACAAACAGCC AGACACTGCT 
CTCCTGAGCT TTCTTGGTGT GCAAAGGAAA TTTTGGCCAG TGACTTTGTC GGTCTTTGGA 
GACACTAAAA AGGTTTTGCC TACTACAAAA GATTCCTGTT TTGCCTCAGC AGTAGAATGT 
CTACAACAAA TCAGCACTAC ATTTACTCCA TCAGACAAAC TTAAAGTTAT CCATCAGACT 
TTTGAAGAGA TATCTCAGAG TGTCCTTGCT ACGCTTCAGG AAGACTTCTT GTGGTCCATG 
GATGACCTGT TTCCCGTTTT CTTATATGTG GTGCTACGGG CCAGGATTAG GAACTTGGGA 
TCTGAAGTGC ATCTCATTGA GGATCTGATG GATCCATATC TCCAGCATGG GGAACAGGGA 
ATCATGTTCA CTACCTTGAA GGTGAGTGTA TTTCACCTTT TTAATAATGC TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_016425193.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016280679.1 Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X6, mRNA.

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CloneID	OMc44705
Clone ID Related Accession (Same CDS sequence)	XM_016425193.1
Accession Version	XM_016425193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008807.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGTCTC TTACTGAAGA ACCAAATCCC 
AGTCTCCCCA GCCCACCAAC CACAAGTACA TCTGCTCTAA ACAGCTTAGT GGTTTCCTGT 
GCCTCCGCGG TTGGTGTGAG AGTAGCGGCT ACATATGAAG CTGGAGCCCT GTCTCTTAAG 
AAAGTAATGA ACTTTTATAG TATAGCTCCT TGTGAAAGTG GACTTCAGTC AGGCAATGCT 
TCCTTGGGCC CAGAAGGATT AAAGGATAGC CGAGAAGAGC AGGTTAAACT AGAATCAATG 
CAAGGAAAAA AGAGTTCAAG TCTTGTGGAT ATTAGAGAAG AGGAATCTGA AGGAGTTAGC 
AGAAGGCTTT CTCTTCCTGG ATTATTATCA CAAGTTTCTC CCAGGCTTCT GAGAAAAGCT 
GCCCGGGCAA AAACACGTAC AGTGGTATTG ACACCTACAT ATAGTGGGGA AGCAGATGCC 
CTCCTCCCTT CTTTAAGGAC TGAAGTGTGG ACTTGGGGAA AAGGAAAAGA AGGACAGCTA 
GGACATGGTG ATGTTTTGCC TAGACTTCAG CCATTATGTG TAAAATGTTT AGATGGTAAA 
GAAGTAATTC AACTGGCAGC TGGTGGTCAT CATTCCCTTG CTCTCACTGC TAAATCCCAG 
GTTTACTCAT GGGGTAGTAA TACTTTTGGT CAACTTGGTC ATTCAGATTC TCCAACTACA 
ATTCCTCGTC TGGCAAAGGT GTGTGATGAA AATGGAATCT GGAGTATAGC TGCTGGGCAG 
GATTATTCCC TTTTTTTGGC AGATACAGAT GATTTCCAGC CTGGATTGTA TTACAGTGGT 
CGGCAAGAAC CTGCTGAAAG TGATCATGAT ATTTGTACTA AGACTGCATC ACTGCTGGGA 
TCCTGTAGTA AACTTGGGTA CATAAGCAGT GTGACAGCAG GAAAAAACAG CTGCCTAGCT 
TTGGTGGACA AAAATATTAT GGGATATATT GCCAGTCTTC ATGAGCTGGC CACTACAGAA 
AGACGGTTCT ATTCAAAACT GAGTGATATT AAATCCCAGA TTCTCAGACC CCTCCTAGGT 
TTAGAAAATT TGGGTACCAC CACTACAGTC CAGTTGTTGC AGGATGTGGC AAGCAGATTC 
AGCAAGCTGT GTTACCTGAT TGGTCAGCAT GGAGCCTCTC TGAGCAGCTT TCTTCATGGA 
ATTAAGGAAG CCAAGAGTTT GGCCATCTTG AAGCATGCAA GTCTCTTCTT GGATAGTTAT 
ACAGAGTATT GCACATCTAT TACAAATTTT GTGGTTATGG GAGGATTCCA ACTTCTGGCC 
AAGCCAGCTA CTGACTTCTT AAATAAAAAC CAAGAGTTAT TACAAGACTT ATCAGAGGTG 
AATGAGGAAA ATATCCAATT GGTGGAACTG CTAAACACTT TGTTTTTTTT GCCAATCAGA 
CGTCTTCATA ATTATGCTCG AGTTTTGCTA AAGCTTGCTA CTTGTTTTGA AGTGACATCT 
CCAGAGTATC ATAAACTGCA GGATTCCAGC TCTTGTTATG AGACTCTTGC TCTCCATCTC 
GGCAGGAAAA GGAAGGAAGC AGAATACACA CTGGGCTTCT GGAAGACCTT TCCTGGGAAA 
ATGACGGATT CCTTGAGGAA ACCAGAACGT AGACTAATCT GTGAGAGTAG TAATCGAGCA 
TTATCTCTAC AACATGCTGG TAGATTCTCT GTGAATTGGT TCATTCTCTT TAATGATTCT 
TTGGTCCATG CCCAGTTCTC CACACATCAT ATTTTCCCCT TATCTACTCT GTGGGTTGAG 
CCTATTTCAG AAGAAGTTGG TAGTGTGAAT GGCTTAAAGA TAACAACACC TGAAGAACAA 
TTTATTCTTG TTTCATCAAC ACCACAGGAA AAAACTAAAT GGTTACGGGC TATAAGCCAA 
GCTGTGGATC AAGCTCTCCG TGGTATGTCT GATTTTCCCC CATATGGAAG TGGAAGCAGT 
GTTCCCAGGC AAGAGCCTCC TATTTCACGA AGTGCCAGAT ACACTTTCTA CAAGGACCCT 
CGACTAAAGG ATGCCATTTA TGATGGACGA TGGCTTTCAG GGAAACCCCA TGGCAGAGGG 
ACTCTGAAAT GGACTGATGG AAAGATGTAT TCTGGTATGT TCAGAAATGG TCTGGAAGAT 
GGGTATGGGG AATACAAAGT CCCAAATAAA GCACTGAACA AGGATGACCA TTATGTGGGA 
TATTGGAAAG AAGGAAAAAT GTGTGGTCAA GGAGTCTACA GCTATGCCAA TGGAGAGGTA 
TATGAAGGCT GCTTTCAGGA TAACATGCGT CACGGTCATG GCCTTCTACG CAGTGGTAAA 
CTGACTTCTT CTTCTCCCAG TATGTTCATT GGCCAATGGG TGATGGATAA AAAGTCTGGA 
TATGGTGTAT TTGATGATAT CACAAGGGGA GAAAAATATA TGGGAATGTG GCAAGATGAT 
ATGTGTCAGG GGAATGGTGT GGTAGTTACC CAGTTTGGAT TATACTATGA AGGAACATTT 
AGCCTTAATA AAATGATGGG AACTGGTATT TTACTCTCAG AAGATGATAC TGTCTACGAG 
GGAGAATTTT CAGATGACTG GACACTTAAT GGAAAGGGAA CACTGACTAT GCCAAATGGT 
GATTACATTG AAGGTCTTTT CAATGGGGAA TGGGGATCTG GGATAAAAAT TACTGGAACA 
TACTTCAAAC CCAGTTTGTA CGAGAGTGAG AAAGACCGCC CCAAAATTCT TAGGAAACTA 
GGAAACCTGG CAGTGCCTGC TGATGAGAAA TGGAAAGCAG TATTTGAAGA ATGCTGGCAC 
CAGTTAGGCT GTGAGAACCC AGGCCAGGGA GAACTCTGGA AAGCATGGGA CAACATCGCT 
GTAGCCCTAA CCACCAATCG TCGCCAGCAC CGAGATAGTC CAGAGATATT AAGTCGTTCA 
CATACCCAGA CATTGGAAAG TTTGGAATTC ATTCCACAGC ATGTTGGTGG TGCCTTCACT 
GTCGAGAAAT ATGATGAAAT AAAGAAATAC TTAATAAAGG CTTGTGACAC TCCTCTGCAC 
CCTTTGTGTC GTCTCATAGA AACCTTGGTT GCAGTGTATA GGATGACCTA TGTGGGAGTG 
GGAGCTAATC GGCGATTATT GCAAGAAGCT GTGAAAGAGA TTCAGTCCTA CCTTAAGCGT 
ATTTTCCAGC TGGTCAGGTT CCTATTTCCT GATCTTCCTG AAGAGGGCAG CACAATTCCA 
TTGTCTGTTC CTCTACCCCA GGAAAGGAAA TCTTTCTGCA CAGGAAAGTC TGATTCTAGG 
TCAGAATCTC CAGAACCAGG CTATGTGGTC ACGAGTTCTG GGTTACTGCT GCCTGTACTG 
TTACCTCGAC TCTACCCACC ACTCTTTATG CTGTATGCCT TGGAGAATGA TAGAGAGGAA 
GATATTTATT GGGAGTGTGT TCTTCGACTC AACAAACAGC CAGACACTGC TCTCCTGAGC 
TTTCTTGGTG TGCAAAGGAA ATTTTGGCCA GTGACTTTGT CGGTCTTTGG AGACACTAAA 
AAGGTTTTGC CTACTACAAA AGATTCCTGT TTTGCCTCAG CAGTAGAATG TCTACAACAA 
ATCAGCACTA CATTTACTCC ATCAGACAAA CTTAAAGTTA TCCATCAGAC TTTTGAAGAG 
ATATCTCAGA GTGTCCTTGC TACGCTTCAG GAAGACTTCT TGTGGTCCAT GGATGACCTG 
TTTCCCGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAACTTGGG ATCTGAAGTG 
CATCTCATTG AGGATCTGAT GGATCCATAT CTCCAGCATG GGGAACAGGG AATCATGTTC 
ACTACCTTGA AGGTGAGTGT ATTTCACCTT TTTAATAATG CTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016280679.1
CDS	825..5792
Translation	MDSPERSSAEAEGAKERGLVHVWQAGFCSITPERLPGWGGKTVLQAALGVSHGVLLTEGGEVYSFGNLPWRSEPEEICPKSPLLENALVGQHVITVATGSFHSGAVTENGMVYMWGQNSAGQCAIANQHHVPEPTPVSISDSETSPLLSVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGDSHSLALVQCLPSQDVKPVPERCNHCSQLLITMTDKEDHVIISDSHCCPLGVALSETQAESHDAITPSTGESLESKNLSKSQGGEKKTLRENGLSVASEVNIENMQPVNAEATHPEQEITEATGIPILGNVPLFPDNHVEKACLQKLSDDSLQGHPETGGEVESLTEEPNPSLPSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSIAPCESGLQSGNASLGPEGLKDSREEQVKLESMQGKKSSSLVDIREEESEGVSRRLSLPGLLSQVSPRLLRKAARAKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIQLAAGGHHSLALTAKSQVYSWGSNTFGQLGHSDSPTTIPRLAKVCDENGIWSIAAGQDYSLFLADTDDFQPGLYYSGRQEPAESDHDICTKTASLLGSCSKLGYISSVTAGKNSCLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLGLENLGTTTTVQLLQDVASRFSKLCYLIGQHGASLSSFLHGIKEAKSLAILKHASLFLDSYTEYCTSITNFVVMGGFQLLAKPATDFLNKNQELLQDLSEVNEENIQLVELLNTLFFLPIRRLHNYARVLLKLATCFEVTSPEYHKLQDSSSCYETLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRLICESSNRALSLQHAGRFSVNWFILFNDSLVHAQFSTHHIFPLSTLWVEPISEEVGSVNGLKITTPEEQFILVSSTPQEKTKWLRAISQAVDQALRGMSDFPPYGSGSSVPRQEPPISRSARYTFYKDPRLKDAIYDGRWLSGKPHGRGTLKWTDGKMYSGMFRNGLEDGYGEYKVPNKALNKDDHYVGYWKEGKMCGQGVYSYANGEVYEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKSGYGVFDDITRGEKYMGMWQDDMCQGNGVVVTQFGLYYEGTFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTMPNGDYIEGLFNGEWGSGIKITGTYFKPSLYESEKDRPKILRKLGNLAVPADEKWKAVFEECWHQLGCENPGQGELWKAWDNIAVALTTNRRQHRDSPEILSRSHTQTLESLEFIPQHVGGAFTVEKYDEIKKYLIKACDTPLHPLCRLIETLVAVYRMTYVGVGANRRLLQEAVKEIQSYLKRIFQLVRFLFPDLPEEGSTIPLSVPLPQERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALENDREEDIYWECVLRLNKQPDTALLSFLGVQRKFWPVTLSVFGDTKKVLPTTKDSCFASAVECLQQISTTFTPSDKLKVIHQTFEEISQSVLATLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKVSVFHLFNNAD

Target ORF information:

RefSeq Version	XM_016425193.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016425193.1

ORF Insert Sequence:

ATGGACTCAC CGGAGAGAAG CTCAGCAGAG GCAGAAGGAG CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATT CTGCTCTATA ACACCCGAGA GACTGCCGGG ATGGGGAGGA 
AAGACTGTGC TTCAAGCAGC CTTAGGCGTG AGTCATGGAG TACTTCTAAC TGAAGGTGGT 
GAAGTCTACA GCTTTGGGAA CCTTCCCTGG AGATCAGAAC CAGAAGAGAT CTGTCCAAAG 
AGTCCTCTTC TAGAAAATGC TTTGGTTGGA CAGCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTAAC TGAGAATGGC ATGGTGTACA TGTGGGGGCA AAATTCTGCT 
GGCCAGTGTG CTATTGCTAA CCAGCACCAT GTACCAGAAC CAACACCCGT CAGTATTTCT 
GACTCTGAGA CAAGCCCTTT GTTATCAGTC AGGATTTTGC AGTTGGCTTG TGGAGAGGAG 
CACACTTTGG CCCTCTCAAT AAGCAGAGAG ATATGGGCAT GGGGTACGGG TTGTCAGTTG 
GGTCTCATCA CCACTACTTT CCCAGTGACA AAACCACAAA AAGTAGAACA TCTCGCTGGG 
CGAGTAGTGC TCCAAGTGGC CTGTGGTGAT TCCCACAGCT TAGCCCTCGT ACAGTGCCTC 
CCCTCACAGG ATGTAAAGCC AGTCCCAGAA AGATGTAATC ACTGCAGCCA GCTCCTGATT 
ACTATGACTG ACAAAGAAGA CCATGTGATC ATTTCTGATA GTCATTGCTG TCCATTAGGT 
GTGGCACTAT CAGAAACCCA GGCAGAAAGC CATGATGCCA TCACTCCATC CACAGGGGAG 
AGTCTGGAAA GCAAAAATTT ATCCAAAAGC CAGGGAGGAG AAAAGAAGAC TCTTAGAGAG 
AATGGTTTGT CTGTTGCTTC CGAAGTGAAT ATTGAAAATA TGCAGCCAGT GAATGCTGAG 
GCCACCCACC CTGAACAAGA GATCACCGAA GCCACAGGAA TTCCCATTTT GGGAAATGTG 
CCATTATTCC CTGACAACCA CGTAGAGAAA GCATGCTTAC AGAAGTTGTC GGATGATTCA 
TTACAAGGAC ATCCTGAGAC AGGTGGCGAG GTAGAGTCTC TTACTGAAGA ACCAAATCCC 
AGTCTCCCCA GCCCACCAAC CACAAGTACA TCTGCTCTAA ACAGCTTAGT GGTTTCCTGT 
GCCTCCGCGG TTGGTGTGAG AGTAGCGGCT ACATATGAAG CTGGAGCCCT GTCTCTTAAG 
AAAGTAATGA ACTTTTATAG TATAGCTCCT TGTGAAAGTG GACTTCAGTC AGGCAATGCT 
TCCTTGGGCC CAGAAGGATT AAAGGATAGC CGAGAAGAGC AGGTTAAACT AGAATCAATG 
CAAGGAAAAA AGAGTTCAAG TCTTGTGGAT ATTAGAGAAG AGGAATCTGA AGGAGTTAGC 
AGAAGGCTTT CTCTTCCTGG ATTATTATCA CAAGTTTCTC CCAGGCTTCT GAGAAAAGCT 
GCCCGGGCAA AAACACGTAC AGTGGTATTG ACACCTACAT ATAGTGGGGA AGCAGATGCC 
CTCCTCCCTT CTTTAAGGAC TGAAGTGTGG ACTTGGGGAA AAGGAAAAGA AGGACAGCTA 
GGACATGGTG ATGTTTTGCC TAGACTTCAG CCATTATGTG TAAAATGTTT AGATGGTAAA 
GAAGTAATTC AACTGGCAGC TGGTGGTCAT CATTCCCTTG CTCTCACTGC TAAATCCCAG 
GTTTACTCAT GGGGTAGTAA TACTTTTGGT CAACTTGGTC ATTCAGATTC TCCAACTACA 
ATTCCTCGTC TGGCAAAGGT GTGTGATGAA AATGGAATCT GGAGTATAGC TGCTGGGCAG 
GATTATTCCC TTTTTTTGGC AGATACAGAT GATTTCCAGC CTGGATTGTA TTACAGTGGT 
CGGCAAGAAC CTGCTGAAAG TGATCATGAT ATTTGTACTA AGACTGCATC ACTGCTGGGA 
TCCTGTAGTA AACTTGGGTA CATAAGCAGT GTGACAGCAG GAAAAAACAG CTGCCTAGCT 
TTGGTGGACA AAAATATTAT GGGATATATT GCCAGTCTTC ATGAGCTGGC CACTACAGAA 
AGACGGTTCT ATTCAAAACT GAGTGATATT AAATCCCAGA TTCTCAGACC CCTCCTAGGT 
TTAGAAAATT TGGGTACCAC CACTACAGTC CAGTTGTTGC AGGATGTGGC AAGCAGATTC 
AGCAAGCTGT GTTACCTGAT TGGTCAGCAT GGAGCCTCTC TGAGCAGCTT TCTTCATGGA 
ATTAAGGAAG CCAAGAGTTT GGCCATCTTG AAGCATGCAA GTCTCTTCTT GGATAGTTAT 
ACAGAGTATT GCACATCTAT TACAAATTTT GTGGTTATGG GAGGATTCCA ACTTCTGGCC 
AAGCCAGCTA CTGACTTCTT AAATAAAAAC CAAGAGTTAT TACAAGACTT ATCAGAGGTG 
AATGAGGAAA ATATCCAATT GGTGGAACTG CTAAACACTT TGTTTTTTTT GCCAATCAGA 
CGTCTTCATA ATTATGCTCG AGTTTTGCTA AAGCTTGCTA CTTGTTTTGA AGTGACATCT 
CCAGAGTATC ATAAACTGCA GGATTCCAGC TCTTGTTATG AGACTCTTGC TCTCCATCTC 
GGCAGGAAAA GGAAGGAAGC AGAATACACA CTGGGCTTCT GGAAGACCTT TCCTGGGAAA 
ATGACGGATT CCTTGAGGAA ACCAGAACGT AGACTAATCT GTGAGAGTAG TAATCGAGCA 
TTATCTCTAC AACATGCTGG TAGATTCTCT GTGAATTGGT TCATTCTCTT TAATGATTCT 
TTGGTCCATG CCCAGTTCTC CACACATCAT ATTTTCCCCT TATCTACTCT GTGGGTTGAG 
CCTATTTCAG AAGAAGTTGG TAGTGTGAAT GGCTTAAAGA TAACAACACC TGAAGAACAA 
TTTATTCTTG TTTCATCAAC ACCACAGGAA AAAACTAAAT GGTTACGGGC TATAAGCCAA 
GCTGTGGATC AAGCTCTCCG TGGTATGTCT GATTTTCCCC CATATGGAAG TGGAAGCAGT 
GTTCCCAGGC AAGAGCCTCC TATTTCACGA AGTGCCAGAT ACACTTTCTA CAAGGACCCT 
CGACTAAAGG ATGCCATTTA TGATGGACGA TGGCTTTCAG GGAAACCCCA TGGCAGAGGG 
ACTCTGAAAT GGACTGATGG AAAGATGTAT TCTGGTATGT TCAGAAATGG TCTGGAAGAT 
GGGTATGGGG AATACAAAGT CCCAAATAAA GCACTGAACA AGGATGACCA TTATGTGGGA 
TATTGGAAAG AAGGAAAAAT GTGTGGTCAA GGAGTCTACA GCTATGCCAA TGGAGAGGTA 
TATGAAGGCT GCTTTCAGGA TAACATGCGT CACGGTCATG GCCTTCTACG CAGTGGTAAA 
CTGACTTCTT CTTCTCCCAG TATGTTCATT GGCCAATGGG TGATGGATAA AAAGTCTGGA 
TATGGTGTAT TTGATGATAT CACAAGGGGA GAAAAATATA TGGGAATGTG GCAAGATGAT 
ATGTGTCAGG GGAATGGTGT GGTAGTTACC CAGTTTGGAT TATACTATGA AGGAACATTT 
AGCCTTAATA AAATGATGGG AACTGGTATT TTACTCTCAG AAGATGATAC TGTCTACGAG 
GGAGAATTTT CAGATGACTG GACACTTAAT GGAAAGGGAA CACTGACTAT GCCAAATGGT 
GATTACATTG AAGGTCTTTT CAATGGGGAA TGGGGATCTG GGATAAAAAT TACTGGAACA 
TACTTCAAAC CCAGTTTGTA CGAGAGTGAG AAAGACCGCC CCAAAATTCT TAGGAAACTA 
GGAAACCTGG CAGTGCCTGC TGATGAGAAA TGGAAAGCAG TATTTGAAGA ATGCTGGCAC 
CAGTTAGGCT GTGAGAACCC AGGCCAGGGA GAACTCTGGA AAGCATGGGA CAACATCGCT 
GTAGCCCTAA CCACCAATCG TCGCCAGCAC CGAGATAGTC CAGAGATATT AAGTCGTTCA 
CATACCCAGA CATTGGAAAG TTTGGAATTC ATTCCACAGC ATGTTGGTGG TGCCTTCACT 
GTCGAGAAAT ATGATGAAAT AAAGAAATAC TTAATAAAGG CTTGTGACAC TCCTCTGCAC 
CCTTTGTGTC GTCTCATAGA AACCTTGGTT GCAGTGTATA GGATGACCTA TGTGGGAGTG 
GGAGCTAATC GGCGATTATT GCAAGAAGCT GTGAAAGAGA TTCAGTCCTA CCTTAAGCGT 
ATTTTCCAGC TGGTCAGGTT CCTATTTCCT GATCTTCCTG AAGAGGGCAG CACAATTCCA 
TTGTCTGTTC CTCTACCCCA GGAAAGGAAA TCTTTCTGCA CAGGAAAGTC TGATTCTAGG 
TCAGAATCTC CAGAACCAGG CTATGTGGTC ACGAGTTCTG GGTTACTGCT GCCTGTACTG 
TTACCTCGAC TCTACCCACC ACTCTTTATG CTGTATGCCT TGGAGAATGA TAGAGAGGAA 
GATATTTATT GGGAGTGTGT TCTTCGACTC AACAAACAGC CAGACACTGC TCTCCTGAGC 
TTTCTTGGTG TGCAAAGGAA ATTTTGGCCA GTGACTTTGT CGGTCTTTGG AGACACTAAA 
AAGGTTTTGC CTACTACAAA AGATTCCTGT TTTGCCTCAG CAGTAGAATG TCTACAACAA 
ATCAGCACTA CATTTACTCC ATCAGACAAA CTTAAAGTTA TCCATCAGAC TTTTGAAGAG 
ATATCTCAGA GTGTCCTTGC TACGCTTCAG GAAGACTTCT TGTGGTCCAT GGATGACCTG 
TTTCCCGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAACTTGGG ATCTGAAGTG 
CATCTCATTG AGGATCTGAT GGATCCATAT CTCCAGCATG GGGAACAGGG AATCATGTTC 
ACTACCTTGA AGGTGAGTGT ATTTCACCTT TTTAATAATG CTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ALS2 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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