DNA Related Biological Terms

The removal of damaged segments, e.g. pyrimidine dimers, from one strand of double-stranded DNA and its correct resynthesis.

(= DNA fingerprinting (DNA profiling))

(= exonuclease footprinting (DNase footprinting))

double-stranded DNA. (see also double helix (Watson-Crick model))

A method for identification of a protein-binding region in a double-stranded DNA fragment. One 5'-end of the DNA fragment is labelled with 32P and the DNA-fragment-protein complex is treated with a 3'-exonuclease to digest from the 3'-end until it meets the region protected by the DNA-binding protein. The labelled strand is characterized subsequently by its size to indicate the distance of the site of the binding protein from the 5'-end of the fragment. The technique is suitable for localization of a binding site to a specific region of a large DNA fragment, whereas footprinting gives the exact sequence of the binding site of a smaller fragment. (see also footprinting)Revzin, A. (ed.) (1993) footprinting of Nucleic Acid-Protein Complexes, Academic Press, San Diego

Automated DNA sequencing using single-lane sequencing; adaptable to automated data collection.

see quadruplex DNA

DNA that has been isolated from a cell and therefore contains introns, as opposed to cDNA.

A form of triplex DNA characterized by Hoogstein base pairing.Yagil, G. (1991) Crit. Rev. Biochem. Mol. Biol. 26, 475-559; Frank-Kamenetskii, M.D. and Mirkin, S.M. (1995) Annu. Rev. Biochem. 64, 65-95; Vasquez, K.M. and Wilson, J.H. (1998) Trends Biochem. Sci. 23, 4-9

A branched double-stranded DNA structure, a chi-form in which each of four polynucleotide chains is complementary to and base-paired with the 3'-end of one polynucleotide and the 5'-end of another; unlike in a recombination intermediate, the junction cannot migrate (i.e. slide its branch point along the duplex) without breaking many more Watson-Crick base-pair hydrogen bonds as it advances than can re-form behind it. (see also Holliday model)