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stoichiometric

A chemical term meaning that the molecules referred to are present in constant proportions; for nucleic acids this generally equates to equal numbers of molecules. Complete digestion of a DNA fragment with a Restriction enzyme dictates that all resulting smaller DNA fragments derived from the original band should be stoichiometric, or present in the same number, with the relative staining intensity of each band proportional to fragment size following gel electrophoresis. Bands that are not present in stoichiometric levels are indicative of digestion artefacts, such as contaminating DNA fragments, incomplete enzyme activity, or contaminating enzyme activity.

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Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

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Bridge the gap between chemically synthesized oligos and bological applications.

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: website@genscript.com

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