Application Note: Overcoming Traditional Limitations: GenScript’s Advanced mRNA Applied Vector for Superior Cloning and Efficient Plasmid Production

Overcoming Traditional Limitations: GenScript’s Advanced mRNA Applied Vector for Superior Cloning and Efficient Plasmid Production

Executive Summary: In response to the growing demand for mRNA-based therapeutics, we have engineered the GS-mK and GS-CMV vectors to address the limitations of traditional vectors such as pUC57 and pVAX1. These novel vectors have been specifically designed to improve cloning efficiency, stabilize poly(A) tails, and increase plasmid yields, thereby enhancing the production process of IVT mRNA.

Highlights:

• Dive into the breakthrough of segmented poly(A) tails: a game-changer in reducing plasmid DNA recombination and skyrocketing cloning efficiency.
• Uncover the art of selecting the perfect poly(A) tail length: striking the ultimate balance between peak mRNA expression and cloning precision.
• Explore our next generation mRNA applied vectors, redefining the landscape of IVT mRNA production with unparalleled standards.