ATP1A1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP1A1
Entrez Gene ID	397481
Full Name	ATPase Na+/K+ transporting subunit alpha 1
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 4 Map Location: 4\|4q1.6-q2.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_021088717.1	XP_020944376.1	sodium/potassium-transporting ATPase subunit alpha-1 isoform X1
NM_214249.1	NP_999414.1	sodium/potassium-transporting ATPase subunit alpha-1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Spin-labeled derivatives of cardiotonic steroids as tools for characterization of the extracellular entrance to the binding site on Na
Guo JH, Jiang RW, Andersen JL, Esmann M, Fedosova NU
The FEBS journal285(12)2292-2305(2018 06)

Interaction of N-terminal peptide analogues of the Na
Nguyen K, Garcia A, Sani MA, Diaz D, Dubey V, Clayton D, Dal Poggetto G, Cornelius F, Payne RJ, Separovic F, Khandelia H, Clarke RJ
Biochimica et biophysica acta. Biomembranes1860(6)1282-1291(2018 Jun)

Src Family Kinase Links Insulin Signaling to Short Term Regulation of Na,K-ATPase in Nonpigmented Ciliary Epithelium.
Shahidullah M, Mandal A, Delamere NA
Journal of cellular physiology232(6)1489-1500(2017 Jun)

Uroguanylin modulates (Na++K+)ATPase in a proximal tubule cell line: Interactions among the cGMP/protein kinase G, cAMP/protein kinase A, and mTOR pathways.
Arnaud-Batista FJ, Peruchetti DB, Abreu TP, do Nascimento NR, Malnic G, Fonteles MC, Caruso-Neves C
Biochimica et biophysica acta1860(7)1431-8(2016 Jul)

The E2P-like state induced by magnesium fluoride complexes in the Na,K-ATPase. Kinetics of formation and interaction with Rb(+).
Montes MR, Ferreira-Gomes MS, Centeno M, Rossi RC
Biochimica et biophysica acta1848(7)1514-23(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ATP1A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP1A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00373	NM_214249.1 Latest version!	Sus scrofa ATPase Na+/K+ transporting subunit alpha 1 (ATP1A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe247249	XM_021088717.1 Latest version!	Sus scrofa ATPase Na+/K+ transporting subunit alpha 1 (ATP1A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP1A1 ( NM_214249.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999414.1 Sus scrofa ATPase Na+/K+ transporting subunit alpha 1 (ATP1A1), mRNA.

Price & Availability↑

CloneID	OSe00373
Clone ID Related Accession (Same CDS sequence)	NM_214249.1
Accession Version	NM_214249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Sus scrofa(pig)
Product	sodium/potassium-transporting ATPase subunit alpha-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X03938.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X03938.1, AK345977.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01048247, SAMN02364669 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAAGG GGGTTGGACG CGATAAATAT GAGCCCGCAG CCGTGTCAGA GCATGGCGAC 
AAAAAGAAGG CCAAGAAGGA GAGGGATATG GATGAGCTGA AGAAGGAAGT TTCTATGGAT 
GACCATAAAC TTAGCCTTGA TGAGCTTCAT CGCAAATACG GAACGGACTT GAGCCGAGGC 
TTAACACCTG CTCGAGCTGC TGAGATCCTA GCCCGAGACG GTCCCAATGC CCTGACACCC 
CCACCCACAA CCCCTGAATG GGTCAAGTTC TGTCGGCAGC TCTTCGGAGG CTTCTCCATG 
TTACTGTGGA TCGGAGCGAT TCTTTGTTTC TTGGCCTATG GCATTCAAGC TGCTACAGAA 
GAGGAACCTC AAAATGATAA TCTGTACCTT GGTGTGGTGC TCTCCGCCGT CGTCATCATA 
ACTGGCTGTT TCTCCTACTA TCAAGAAGCG AAAAGCTCAA AGATCATGGA ATCCTTCAAA 
AACATGGTTC CTCAGCAAGC CCTCGTGATT CGAAATGGTG AAAAGATGAG CATAAATGCA 
GAGGAAGTCG TCGTCGGGGA TCTGGTGGAG GTGAAGGGAG GGGATCGAAT CCCTGCTGAC 
CTCAGGATCA TATCTGCGAA CGGCTGCAAG GTGGACAACT CCTCCCTCAC TGGTGAATCA 
GAACCGCAGA CCAGGTCTCC AGATTTCACC AATGAGAACC CCCTGGAGAC TAGGAACATC 
GCCTTTTTTT CAACCAACTG CGTTGAAGGC ACTGCACGTG GTATTGTGGT GTACACTGGC 
GATCGCACCG TGATGGGCAG AATCGCTACC CTTGCTTCCG GGCTGGAAGG GGGCCAGACT 
CCCATCGCTG CGGAGATTGA ACATTTTATC CACATCATCA CGGGCGTGGC CGTGTTCCTG 
GGCGTGTCCT TCTTCATCCT TTCTCTGATC CTCGAGTACA CCTGGCTCGA GGCCGTCATC 
TTCCTCATCG GGATCATTGT AGCCAACGTG CCTGAAGGTT TGCTGGCCAC CGTCACGGTG 
TGCTTGACCC TGACTGCCAA GCGCATGGCC AGGAAGAACT GCCTTGTGAA GAACTTGGAG 
GCTGTGGAGA CCCTGGGGTC CACATCCACC ATCTGCTCAG ACAAAACCGG AACCCTCACC 
CAGAACCGAA TGACAGTGGC CCACATGTGG TCCGACAATC AAATCCACGA GGCTGACACG 
ACGGAAAATC AGAGCGGTGT CTCATTCGAC AAGACTTCGG CCACCTGGCT TGCTCTGTCC 
AGAATTGCAG GTCTTTGTAA CAGGGCAGTG TTCCAGGCCA ACCAGGAAAA CCTACCTATC 
CTGAAGCGGG CAGTGGCGGG CGACGCCTCC GAGTCCGCGC TCTTAAAGTG CATCGAGCTG 
TGCTGTGGGT CCGTGAAGGA GATGAGGGAG CGATACACCA AGATCGTCGA GATTCCCTTC 
AACTCCACCA ACAAGTACCA GCTGTCCATC CACAAGAACC CCAACACGGC TGAGCCCCGG 
CACCTGCTGG TGATGAAAGG TGCTCCAGAA AGGATCCTGG ACCGCTGCAG CTCCATCCTC 
ATCCACGGCA AGGAGCAGCC CCTAGACGAG GAGCTGAAGG ACGCCTTTCA GAACGCCTAC 
CTGGAGCTGG GTGGCCTCGG GGAACGCGTG CTGGGTTTCT GCCACCTTTT CCTGCCGGAC 
GAGCAGTTCC CCGAAGGCTT CCAGTTTGAC ACCGACGATG TGAATTTCCC TCTCGATAAT 
CTCTGCTTCG TTGGGCTCAT CTCCATGATT GACCCACCGC GAGCGGCCGT CCCGGATGCC 
GTGGGCAAAT GTCGAAGCGC TGGCATTAAG GTCATCATGG TCACCGGCGA TCATCCCATC 
ACAGCCAAAG CCATTGCCAA AGGTGTGGGC ATCATCTCGG AAGGCAATGA AACGGTCGAA 
GACATCGCTG CCCGCCTCAA CATCCCAGTG AGCCAGGTGA ACCCCAGGGA TGCCAAGGCC 
TGCGTGGTCC ATGGAAGCGA TCTGAAAGAC ATGACCTCGG AGCAGCTGGA TGACATCTTG 
AAGTACCACA CGGAGATCGT GTTTGCCCGG ACGTCTCCTC AGCAGAAGCT CATCATTGTG 
GAAGGCTGCC AGAGACAGGG CGCCATCGTG GCCGTGACTG GCGACGGTGT CAATGACTCT 
CCCGCTTCGA AGAAGGCAGA CATCGGGGTT GCCATGGGGA TTGCTGGCTC GGACGTGTCC 
AAGCAAGCTG CTGACATGAT CCTCCTGGAT GACAACTTCG CCTCCATTGT GACGGGAGTA 
GAGGAAGGTC GTCTGATCTT TGATAACTTG AAGAAATCCA TTGCCTACAC CCTCACCAGT 
AACATTCCAG AGATCACCCC CTTCCTGATA TTTATTATTG CGAACATTCC ACTGCCCCTG 
GGCACCGTCA CCATCCTCTG CATCGACTTG GGCACAGACA TGGTTCCTGC CATCTCCCTG 
GCGTATGAGC AGGCGGAGAG CGACATCATG AAGAGGCAGC CCCGAAACCC CAAGACAGAC 
AAACTCGTCA ATGAGCAGCT CATCAGCATG GCCTACGGAC AGATAGGTAT GATCCAGGCC 
CTGGGCGGCT TCTTCACTTA CTTTGTGATC CTGGCTGAGA ACGGCTTCCT CCCGATTCAC 
CTGCTGGGCC TCCGGGTGAA CTGGGATGAC CGCTGGATCA ACGACGTGGA GGACAGCTAC 
GGGCAGCAGT GGACCTACGA ACAGAGGAAG ATCGTGGAGT TCACCTGCCA CACGCCCTTC 
TTTGTCACCA TCGTGGTGGT GCAGTGGGCC GACTTGGTCA TCTGCAAGAC CCGGAGGAAT 
TCCGTCTTCC AGCAGGGGAT GAAGAACAAA ATCTTGATCT TTGGCCTCTT CGAAGAGACG 
GCCCTGGCTG CTTTCCTCTC CTACTGCCCC GGAATGGGCG TGGCCCTGAG GATGTACCCC 
CTCAAACCTA CCTGGTGGTT CTGTGCCTTC CCCTACTCGC TCCTCATCTT CGTCTATGAC 
GAAGTCAGGA AGCTCATCAT CAGGCGACGC CCTGGCGGCT GGGTGGAGAA GGAAACCTAC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999414.1
CDS	34..3099
Translation	MGKGVGRDKYEPAAVSEHGDKKKAKKERDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAEILARDGPNALTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWSDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYTKIVEIPFNSTNKYQLSIHKNPNTAEPRHLLVMKGAPERILDRCSSILIHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPLDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPASKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNEQLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVNWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTPFFVTIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

Target ORF information:

RefSeq Version	NM_214249.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa ATPase Na+/K+ transporting subunit alpha 1 (ATP1A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214249.1

ORF Insert Sequence:

ATGGGGAAGG GGGTTGGACG CGATAAATAT GAGCCCGCAG CCGTGTCAGA GCATGGCGAC 
AAAAAGAAGG CCAAGAAGGA GAGGGATATG GATGAGCTGA AGAAGGAAGT TTCTATGGAT 
GACCATAAAC TTAGCCTTGA TGAGCTTCAT CGCAAATACG GAACGGACTT GAGCCGAGGC 
TTAACACCTG CTCGAGCTGC TGAGATCCTA GCCCGAGACG GTCCCAATGC CCTGACACCC 
CCACCCACAA CCCCTGAATG GGTCAAGTTC TGTCGGCAGC TCTTCGGAGG CTTCTCCATG 
TTACTGTGGA TCGGAGCGAT TCTTTGTTTC TTGGCCTATG GCATTCAAGC TGCTACAGAA 
GAGGAACCTC AAAATGATAA TCTGTACCTT GGTGTGGTGC TCTCCGCCGT CGTCATCATA 
ACTGGCTGTT TCTCCTACTA TCAAGAAGCG AAAAGCTCAA AGATCATGGA ATCCTTCAAA 
AACATGGTTC CTCAGCAAGC CCTCGTGATT CGAAATGGTG AAAAGATGAG CATAAATGCA 
GAGGAAGTCG TCGTCGGGGA TCTGGTGGAG GTGAAGGGAG GGGATCGAAT CCCTGCTGAC 
CTCAGGATCA TATCTGCGAA CGGCTGCAAG GTGGACAACT CCTCCCTCAC TGGTGAATCA 
GAACCGCAGA CCAGGTCTCC AGATTTCACC AATGAGAACC CCCTGGAGAC TAGGAACATC 
GCCTTTTTTT CAACCAACTG CGTTGAAGGC ACTGCACGTG GTATTGTGGT GTACACTGGC 
GATCGCACCG TGATGGGCAG AATCGCTACC CTTGCTTCCG GGCTGGAAGG GGGCCAGACT 
CCCATCGCTG CGGAGATTGA ACATTTTATC CACATCATCA CGGGCGTGGC CGTGTTCCTG 
GGCGTGTCCT TCTTCATCCT TTCTCTGATC CTCGAGTACA CCTGGCTCGA GGCCGTCATC 
TTCCTCATCG GGATCATTGT AGCCAACGTG CCTGAAGGTT TGCTGGCCAC CGTCACGGTG 
TGCTTGACCC TGACTGCCAA GCGCATGGCC AGGAAGAACT GCCTTGTGAA GAACTTGGAG 
GCTGTGGAGA CCCTGGGGTC CACATCCACC ATCTGCTCAG ACAAAACCGG AACCCTCACC 
CAGAACCGAA TGACAGTGGC CCACATGTGG TCCGACAATC AAATCCACGA GGCTGACACG 
ACGGAAAATC AGAGCGGTGT CTCATTCGAC AAGACTTCGG CCACCTGGCT TGCTCTGTCC 
AGAATTGCAG GTCTTTGTAA CAGGGCAGTG TTCCAGGCCA ACCAGGAAAA CCTACCTATC 
CTGAAGCGGG CAGTGGCGGG CGACGCCTCC GAGTCCGCGC TCTTAAAGTG CATCGAGCTG 
TGCTGTGGGT CCGTGAAGGA GATGAGGGAG CGATACACCA AGATCGTCGA GATTCCCTTC 
AACTCCACCA ACAAGTACCA GCTGTCCATC CACAAGAACC CCAACACGGC TGAGCCCCGG 
CACCTGCTGG TGATGAAAGG TGCTCCAGAA AGGATCCTGG ACCGCTGCAG CTCCATCCTC 
ATCCACGGCA AGGAGCAGCC CCTAGACGAG GAGCTGAAGG ACGCCTTTCA GAACGCCTAC 
CTGGAGCTGG GTGGCCTCGG GGAACGCGTG CTGGGTTTCT GCCACCTTTT CCTGCCGGAC 
GAGCAGTTCC CCGAAGGCTT CCAGTTTGAC ACCGACGATG TGAATTTCCC TCTCGATAAT 
CTCTGCTTCG TTGGGCTCAT CTCCATGATT GACCCACCGC GAGCGGCCGT CCCGGATGCC 
GTGGGCAAAT GTCGAAGCGC TGGCATTAAG GTCATCATGG TCACCGGCGA TCATCCCATC 
ACAGCCAAAG CCATTGCCAA AGGTGTGGGC ATCATCTCGG AAGGCAATGA AACGGTCGAA 
GACATCGCTG CCCGCCTCAA CATCCCAGTG AGCCAGGTGA ACCCCAGGGA TGCCAAGGCC 
TGCGTGGTCC ATGGAAGCGA TCTGAAAGAC ATGACCTCGG AGCAGCTGGA TGACATCTTG 
AAGTACCACA CGGAGATCGT GTTTGCCCGG ACGTCTCCTC AGCAGAAGCT CATCATTGTG 
GAAGGCTGCC AGAGACAGGG CGCCATCGTG GCCGTGACTG GCGACGGTGT CAATGACTCT 
CCCGCTTCGA AGAAGGCAGA CATCGGGGTT GCCATGGGGA TTGCTGGCTC GGACGTGTCC 
AAGCAAGCTG CTGACATGAT CCTCCTGGAT GACAACTTCG CCTCCATTGT GACGGGAGTA 
GAGGAAGGTC GTCTGATCTT TGATAACTTG AAGAAATCCA TTGCCTACAC CCTCACCAGT 
AACATTCCAG AGATCACCCC CTTCCTGATA TTTATTATTG CGAACATTCC ACTGCCCCTG 
GGCACCGTCA CCATCCTCTG CATCGACTTG GGCACAGACA TGGTTCCTGC CATCTCCCTG 
GCGTATGAGC AGGCGGAGAG CGACATCATG AAGAGGCAGC CCCGAAACCC CAAGACAGAC 
AAACTCGTCA ATGAGCAGCT CATCAGCATG GCCTACGGAC AGATAGGTAT GATCCAGGCC 
CTGGGCGGCT TCTTCACTTA CTTTGTGATC CTGGCTGAGA ACGGCTTCCT CCCGATTCAC 
CTGCTGGGCC TCCGGGTGAA CTGGGATGAC CGCTGGATCA ACGACGTGGA GGACAGCTAC 
GGGCAGCAGT GGACCTACGA ACAGAGGAAG ATCGTGGAGT TCACCTGCCA CACGCCCTTC 
TTTGTCACCA TCGTGGTGGT GCAGTGGGCC GACTTGGTCA TCTGCAAGAC CCGGAGGAAT 
TCCGTCTTCC AGCAGGGGAT GAAGAACAAA ATCTTGATCT TTGGCCTCTT CGAAGAGACG 
GCCCTGGCTG CTTTCCTCTC CTACTGCCCC GGAATGGGCG TGGCCCTGAG GATGTACCCC 
CTCAAACCTA CCTGGTGGTT CTGTGCCTTC CCCTACTCGC TCCTCATCTT CGTCTATGAC 
GAAGTCAGGA AGCTCATCAT CAGGCGACGC CCTGGCGGCT GGGTGGAGAA GGAAACCTAC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP1A1 ( XM_021088717.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020944376.1 Sus scrofa ATPase Na+/K+ transporting subunit alpha 1 (ATP1A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe247249
Clone ID Related Accession (Same CDS sequence)	XM_021088717.1
Accession Version	XM_021088717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	sodium/potassium-transporting ATPase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTA AGGTTGGACG CGATAAATAT GAGCCCGCAG CCGTGTCAGA GCATGGCGAC 
AAAAAGAAGG CCAAGAAGGA GAGGGATATG GATGAGCTGA AGAAGGAAGT TTCTATGGAT 
GACCATAAAC TTAGCCTTGA TGAGCTTCAT CGCAAATACG GAACGGACTT GAGCCGAGGC 
TTAACACCTG CTCGAGCTGC TGAGATCCTG GCCCGAGACG GTCCCAATGC CCTGACGCCC 
CCTCCCACAA CCCCCGAATG GGTCAAGTTC TGTCGGCAGC TCTTCGGAGG CTTCTCCATG 
TTACTGTGGA TCGGAGCGAT TCTTTGTTTC TTGGCCTATG GCATTCAAGC TGCTACCGAA 
GAGGAACCTC AAAATGATAA TCTGTACCTT GGTGTGGTGC TCTCCGCCGT CGTCATCATA 
ACTGGCTGTT TCTCCTACTA TCAAGAAGCG AAAAGCTCAA AGATCATGGA ATCCTTCAAA 
AACATGGTTC CTCAGCAAGC CCTGGTGATT CGAAATGGTG AAAAGATGAG CATAAATGCA 
GAGGAAGTCG TCGTCGGGGA TCTGGTGGAG GTGAAGGGAG GGGATCGAAT CCCTGCTGAC 
CTCAGGATCA TATCTGCGAA CGGCTGCAAG GTGGACAACT CCTCCCTCAC CGGTGAATCA 
GAACCGCAGA CCAGGTCTCC AGATTTCACC AATGAGAACC CCCTGGAGAC TAGAAACATC 
GCCTTTTTTT CAACCAACTG TGTTGAAGGC ACTGCACGTG GTATTGTGGT GTACACTGGC 
GATCGCACCG TGATGGGCAG AATCGCTACC CTTGCTTCCG GGCTGGAAGG GGGCCAGACT 
CCCATCGCTG CGGAGATTGA ACATTTTATC CACATCATCA CGGGCGTGGC CGTGTTCCTG 
GGCGTGTCCT TCTTCATCCT TTCTCTGATC CTCGAGTACA CCTGGCTCGA GGCCGTCATC 
TTCCTCATCG GGATCATTGT AGCCAACGTG CCTGAAGGTT TGCTGGCCAC CGTCACGGTG 
TGCTTGACCC TGACTGCCAA GCGCATGGCC AGGAAGAACT GCCTTGTGAA GAACTTGGAG 
GCTGTGGAGA CCCTGGGGTC CACATCCACC ATCTGCTCAG ACAAAACCGG AACCCTCACC 
CAGAACCGAA TGACAGTGGC CCACATGTGG TTCGACAATC AAATCCACGA GGCTGACACG 
ACGGAAAATC AGAGCGGTGT CTCATTCGAC AAGACTTCGG CCACCTGGCT TGCTCTGTCC 
AGAATTGCAG GTCTTTGTAA CAGGGCAGTG TTCCAGGCCA ACCAGGAAAA CCTACCTATC 
CTGAAGCGGG CAGTGGCGGG CGACGCCTCC GAGTCCGCGC TCTTAAAGTG CATCGAGCTG 
TGCTGCGGGT CCGTGAAGGA GATGAGGGAG CGATACACCA AGATCGTCGA GATTCCCTTC 
AACTCCACCA ACAAGTACCA GCTGTCCATC CACAAGAACC CCAACACGGC TGAGCCCCGG 
CACCTGCTGG TGATGAAAGG TGCTCCAGAA AGGATCCTGG ACCGCTGCAG CTCCATCCTC 
ATCCACGGCA AGGAGCAGCC CCTGGACGAG GAGCTGAAGG ACGCCTTTCA GAACGCCTAC 
CTGGAGCTGG GTGGCCTCGG GGAACGCGTG CTGGGTTTCT GCCACCTTTT CCTGCCGGAC 
GAGCAGTTCC CCGAAGGCTT CCAGTTTGAC ACCGACGATG TGAATTTCCC TCTCGATAAT 
CTCTGCTTCG TTGGGCTCAT CTCCATGATT GACCCACCGC GAGCGGCCGT CCCGGATGCC 
GTGGGCAAAT GTCGAAGCGC TGGCATTAAG GTCATCATGG TCACCGGCGA TCATCCCATC 
ACAGCCAAAG CCATTGCCAA AGGTGTGGGC ATCATCTCGG AAGGCAATGA AACGGTCGAA 
GACATCGCTG CCCGCCTCAA CATCCCAGTG AGCCAGGTGA ACCCCAGGGA TGCCAAGGCC 
TGCGTGGTCC ATGGAAGCGA TCTGAAAGAC ATGACCTCGG AGCAGCTGGA TGACATCTTG 
AAGTACCACA CGGAGATCGT GTTTGCCCGG ACGTCTCCTC AGCAGAAGCT CATCATTGTG 
GAAGGCTGCC AGAGACAGGG CGCCATCGTG GCCGTGACTG GCGACGGTGT CAATGACTCT 
CCCGCTCTGA AGAAGGCAGA CATCGGGGTT GCCATGGGGA TTGCTGGCTC GGACGTGTCC 
AAGCAAGCTG CTGACATGAT CCTCCTGGAT GACAACTTCG CCTCCATTGT GACGGGAGTA 
GAGGAAGGTC GTCTGATCTT TGATAACTTG AAGAAATCCA TTGCCTACAC CCTCACCAGT 
AACATTCCAG AGATCACCCC CTTCCTGATA TTTATTATTG CGAACATTCC ACTGCCCCTG 
GGCACCGTCA CCATCCTCTG CATCGACTTG GGCACAGACA TGGTTCCTGC CATCTCCCTG 
GCGTATGAGC AGGCGGAGAG CGACATCATG AAGAGGCAGC CCCGAAACCC CAAGACAGAC 
AAACTCGTCA ATGAGCGACT CATCAGCATG GCCTACGGAC AGATAGGTAT GATCCAGGCC 
CTGGGCGGCT TCTTCACTTA CTTTGTGATC CTGGCTGAGA ACGGCTTCCT CCCGATTCAC 
CTGCTGGGCC TCCGGGTGAA CTGGGATGAC CGCTGGATCA ACGACGTGGA GGACAGCTAC 
GGGCAGCAGT GGACCTACGA ACAGAGGAAG ATCGTGGAGT TCACCTGCCA CACGGCCTTC 
TTTGTCAGCA TCGTGGTGGT GCAGTGGGCC GACTTGGTCA TCTGCAAGAC CCGGAGGAAT 
TCCGTCTTCC AGCAGGGGAT GAAGAACAAA ATCTTGATCT TTGGCCTCTT CGAAGAGACG 
GCCCTGGCTG CTTTCCTCTC CTACTGCCCC GGAATGGGCG TGGCCCTGAG GATGTACCCC 
CTCAAACCTA CCTGGTGGTT CTGTGCCTTC CCCTACTCGC TCCTCATCTT CGTCTATGAC 
GAAGTCAGGA AGCTCATCAT CAGGCGACGC CCTGGCGGCT GGGTGGAGAA GGAAACCTAC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020944376.1
CDS	8..3073
Translation	MAFKVGRDKYEPAAVSEHGDKKKAKKERDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAEILARDGPNALTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYTKIVEIPFNSTNKYQLSIHKNPNTAEPRHLLVMKGAPERILDRCSSILIHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPLDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVNWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

Target ORF information:

RefSeq Version	XM_021088717.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa ATPase Na+/K+ transporting subunit alpha 1 (ATP1A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021088717.1

ORF Insert Sequence:

ATGGCCTTTA AGGTTGGACG CGATAAATAT GAGCCCGCAG CCGTGTCAGA GCATGGCGAC 
AAAAAGAAGG CCAAGAAGGA GAGGGATATG GATGAGCTGA AGAAGGAAGT TTCTATGGAT 
GACCATAAAC TTAGCCTTGA TGAGCTTCAT CGCAAATACG GAACGGACTT GAGCCGAGGC 
TTAACACCTG CTCGAGCTGC TGAGATCCTG GCCCGAGACG GTCCCAATGC CCTGACGCCC 
CCTCCCACAA CCCCCGAATG GGTCAAGTTC TGTCGGCAGC TCTTCGGAGG CTTCTCCATG 
TTACTGTGGA TCGGAGCGAT TCTTTGTTTC TTGGCCTATG GCATTCAAGC TGCTACCGAA 
GAGGAACCTC AAAATGATAA TCTGTACCTT GGTGTGGTGC TCTCCGCCGT CGTCATCATA 
ACTGGCTGTT TCTCCTACTA TCAAGAAGCG AAAAGCTCAA AGATCATGGA ATCCTTCAAA 
AACATGGTTC CTCAGCAAGC CCTGGTGATT CGAAATGGTG AAAAGATGAG CATAAATGCA 
GAGGAAGTCG TCGTCGGGGA TCTGGTGGAG GTGAAGGGAG GGGATCGAAT CCCTGCTGAC 
CTCAGGATCA TATCTGCGAA CGGCTGCAAG GTGGACAACT CCTCCCTCAC CGGTGAATCA 
GAACCGCAGA CCAGGTCTCC AGATTTCACC AATGAGAACC CCCTGGAGAC TAGAAACATC 
GCCTTTTTTT CAACCAACTG TGTTGAAGGC ACTGCACGTG GTATTGTGGT GTACACTGGC 
GATCGCACCG TGATGGGCAG AATCGCTACC CTTGCTTCCG GGCTGGAAGG GGGCCAGACT 
CCCATCGCTG CGGAGATTGA ACATTTTATC CACATCATCA CGGGCGTGGC CGTGTTCCTG 
GGCGTGTCCT TCTTCATCCT TTCTCTGATC CTCGAGTACA CCTGGCTCGA GGCCGTCATC 
TTCCTCATCG GGATCATTGT AGCCAACGTG CCTGAAGGTT TGCTGGCCAC CGTCACGGTG 
TGCTTGACCC TGACTGCCAA GCGCATGGCC AGGAAGAACT GCCTTGTGAA GAACTTGGAG 
GCTGTGGAGA CCCTGGGGTC CACATCCACC ATCTGCTCAG ACAAAACCGG AACCCTCACC 
CAGAACCGAA TGACAGTGGC CCACATGTGG TTCGACAATC AAATCCACGA GGCTGACACG 
ACGGAAAATC AGAGCGGTGT CTCATTCGAC AAGACTTCGG CCACCTGGCT TGCTCTGTCC 
AGAATTGCAG GTCTTTGTAA CAGGGCAGTG TTCCAGGCCA ACCAGGAAAA CCTACCTATC 
CTGAAGCGGG CAGTGGCGGG CGACGCCTCC GAGTCCGCGC TCTTAAAGTG CATCGAGCTG 
TGCTGCGGGT CCGTGAAGGA GATGAGGGAG CGATACACCA AGATCGTCGA GATTCCCTTC 
AACTCCACCA ACAAGTACCA GCTGTCCATC CACAAGAACC CCAACACGGC TGAGCCCCGG 
CACCTGCTGG TGATGAAAGG TGCTCCAGAA AGGATCCTGG ACCGCTGCAG CTCCATCCTC 
ATCCACGGCA AGGAGCAGCC CCTGGACGAG GAGCTGAAGG ACGCCTTTCA GAACGCCTAC 
CTGGAGCTGG GTGGCCTCGG GGAACGCGTG CTGGGTTTCT GCCACCTTTT CCTGCCGGAC 
GAGCAGTTCC CCGAAGGCTT CCAGTTTGAC ACCGACGATG TGAATTTCCC TCTCGATAAT 
CTCTGCTTCG TTGGGCTCAT CTCCATGATT GACCCACCGC GAGCGGCCGT CCCGGATGCC 
GTGGGCAAAT GTCGAAGCGC TGGCATTAAG GTCATCATGG TCACCGGCGA TCATCCCATC 
ACAGCCAAAG CCATTGCCAA AGGTGTGGGC ATCATCTCGG AAGGCAATGA AACGGTCGAA 
GACATCGCTG CCCGCCTCAA CATCCCAGTG AGCCAGGTGA ACCCCAGGGA TGCCAAGGCC 
TGCGTGGTCC ATGGAAGCGA TCTGAAAGAC ATGACCTCGG AGCAGCTGGA TGACATCTTG 
AAGTACCACA CGGAGATCGT GTTTGCCCGG ACGTCTCCTC AGCAGAAGCT CATCATTGTG 
GAAGGCTGCC AGAGACAGGG CGCCATCGTG GCCGTGACTG GCGACGGTGT CAATGACTCT 
CCCGCTCTGA AGAAGGCAGA CATCGGGGTT GCCATGGGGA TTGCTGGCTC GGACGTGTCC 
AAGCAAGCTG CTGACATGAT CCTCCTGGAT GACAACTTCG CCTCCATTGT GACGGGAGTA 
GAGGAAGGTC GTCTGATCTT TGATAACTTG AAGAAATCCA TTGCCTACAC CCTCACCAGT 
AACATTCCAG AGATCACCCC CTTCCTGATA TTTATTATTG CGAACATTCC ACTGCCCCTG 
GGCACCGTCA CCATCCTCTG CATCGACTTG GGCACAGACA TGGTTCCTGC CATCTCCCTG 
GCGTATGAGC AGGCGGAGAG CGACATCATG AAGAGGCAGC CCCGAAACCC CAAGACAGAC 
AAACTCGTCA ATGAGCGACT CATCAGCATG GCCTACGGAC AGATAGGTAT GATCCAGGCC 
CTGGGCGGCT TCTTCACTTA CTTTGTGATC CTGGCTGAGA ACGGCTTCCT CCCGATTCAC 
CTGCTGGGCC TCCGGGTGAA CTGGGATGAC CGCTGGATCA ACGACGTGGA GGACAGCTAC 
GGGCAGCAGT GGACCTACGA ACAGAGGAAG ATCGTGGAGT TCACCTGCCA CACGGCCTTC 
TTTGTCAGCA TCGTGGTGGT GCAGTGGGCC GACTTGGTCA TCTGCAAGAC CCGGAGGAAT 
TCCGTCTTCC AGCAGGGGAT GAAGAACAAA ATCTTGATCT TTGGCCTCTT CGAAGAGACG 
GCCCTGGCTG CTTTCCTCTC CTACTGCCCC GGAATGGGCG TGGCCCTGAG GATGTACCCC 
CTCAAACCTA CCTGGTGGTT CTGTGCCTTC CCCTACTCGC TCCTCATCTT CGTCTATGAC 
GAAGTCAGGA AGCTCATCAT CAGGCGACGC CCTGGCGGCT GGGTGGAGAA GGAAACCTAC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spin-labeled derivatives of cardiotonic steroids as tools for characterization of the extracellular entrance to the binding site on Na
The FEBS journal285(12)2292-2305(2018 06)
Guo JH,Jiang RW,Andersen JL,Esmann M,Fedosova NU

Interaction of N-terminal peptide analogues of the Na
Biochimica et biophysica acta. Biomembranes1860(6)1282-1291(2018 Jun)
Nguyen K,Garcia A,Sani MA,Diaz D,Dubey V,Clayton D,Dal Poggetto G,Cornelius F,Payne RJ,Separovic F,Khandelia H,Clarke RJ

Src Family Kinase Links Insulin Signaling to Short Term Regulation of Na,K-ATPase in Nonpigmented Ciliary Epithelium.
Journal of cellular physiology232(6)1489-1500(2017 Jun)
Shahidullah M,Mandal A,Delamere NA

Uroguanylin modulates (Na++K+)ATPase in a proximal tubule cell line: Interactions among the cGMP/protein kinase G, cAMP/protein kinase A, and mTOR pathways.
Biochimica et biophysica acta1860(7)1431-8(2016 Jul)
Arnaud-Batista FJ,Peruchetti DB,Abreu TP,do Nascimento NR,Malnic G,Fonteles MC,Caruso-Neves C

The E2P-like state induced by magnesium fluoride complexes in the Na,K-ATPase. Kinetics of formation and interaction with Rb(+).
Biochimica et biophysica acta1848(7)1514-23(2015 Jul)
Montes MR,Ferreira-Gomes MS,Centeno M,Rossi RC

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ATP1A1 cDNA ORF clone, Sus scrofa(pig)

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Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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