TJP1 gene cDNA ORF clone, Sus scrofa(pig)

mRNA	Protein	Name
XM_021098843.1	XP_020954502.1	tight junction protein ZO-1 isoform X4
XM_021098845.1	XP_020954504.1	tight junction protein ZO-1 isoform X5
XM_003480423.3	XP_003480471.2	tight junction protein ZO-1
XM_021098866.1	XP_020954525.1	tight junction protein ZO-1 isoform X9
XM_021098878.1	XP_020954537.1	tight junction protein ZO-1 isoform X11
XM_003480423.4	XP_003480471.3	tight junction protein ZO-1 isoform X10
XM_021098827.1	XP_020954486.1	tight junction protein ZO-1 isoform X1
XM_021098836.1	XP_020954495.1	tight junction protein ZO-1 isoform X3
XM_021098883.1	XP_020954542.1	tight junction protein ZO-1 isoform X12
XM_021098891.1	XP_020954550.1	tight junction protein ZO-1 isoform X14
XM_021098896.1	XP_020954555.1	tight junction protein ZO-1 isoform X15
XM_021098833.1	XP_020954492.1	tight junction protein ZO-1 isoform X2
XM_021098848.1	XP_020954507.1	tight junction protein ZO-1 isoform X6
XM_021098860.1	XP_020954519.1	tight junction protein ZO-1 isoform X8
XM_021098886.1	XP_020954545.1	tight junction protein ZO-1 isoform X13
XM_021098856.1	XP_020954515.1	tight junction protein ZO-1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe230352	XM_021098827.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230353	XM_021098833.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230354	XM_021098836.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230355	XM_021098843.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230356	XM_021098845.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230357	XM_021098848.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230358	XM_021098856.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230359	XM_021098860.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230360	XM_021098866.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230361	XM_003480423.4 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230362	XM_021098878.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230363	XM_021098883.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230364	XM_021098886.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230365	XM_021098891.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe230366	XM_021098896.1 Latest version!	Sus scrofa tight junction protein 1 (TJP1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

TJP1 ( XM_021098827.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954486.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe230352
Clone ID Related Accession (Same CDS sequence)	XM_021098827.1
Accession Version	XM_021098827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGGCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAC CAGCCTCTGC TGTTAATCCT 
AATGTAAAAT TAGGCAGTGT CAGACTGGAG GAGCCCACCT CCGCTCCTCC CACCGCTGAC 
CCCCTACATG CTGACTCTTT AAGAACACCA AGCCCTGAGG CAGCTCACAC AGTGCCCAGA 
GACCAAGAGC CGTCCCTGTC GCCGCATGTA GACCCGGCCA AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954486.1
CDS	68..5656
Translation	MRRLEGLCLRRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQKAEASSPGPYLSPETNPASPASAVNPNVKLGSVRLEEPTSAPPTADPLHADSLRTPSPEAAHTVPRDQEPSLSPHVDPAKVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098827.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098827.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGGCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAC CAGCCTCTGC TGTTAATCCT 
AATGTAAAAT TAGGCAGTGT CAGACTGGAG GAGCCCACCT CCGCTCCTCC CACCGCTGAC 
CCCCTACATG CTGACTCTTT AAGAACACCA AGCCCTGAGG CAGCTCACAC AGTGCCCAGA 
GACCAAGAGC CGTCCCTGTC GCCGCATGTA GACCCGGCCA AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098833.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954492.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OSe230353
Clone ID Related Accession (Same CDS sequence)	XM_021098833.1
Accession Version	XM_021098833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGGCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAC CAGCCTCTGC TGTTAATCCT 
AATGTAAAAT TAGGCAGTGT CAGACTGGAG GAGCCCACCT CCGCTCCTCC CACCGCTGAC 
CCCCTACATG CTGACTCTTT AAGAACACCA AGCCCTGAGG CAGCTCACAC AGTGCCCAGA 
GACCAAGAGC CGTCCCTGTC GCCGCATGTA GACCCGGCCA AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954492.1
CDS	68..5617
Translation	MRRLEGLCLRRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQKAEASSPGPYLSPETNPASPASAVNPNVKLGSVRLEEPTSAPPTADPLHADSLRTPSPEAAHTVPRDQEPSLSPHVDPAKVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098833.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098833.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGGCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAC CAGCCTCTGC TGTTAATCCT 
AATGTAAAAT TAGGCAGTGT CAGACTGGAG GAGCCCACCT CCGCTCCTCC CACCGCTGAC 
CCCCTACATG CTGACTCTTT AAGAACACCA AGCCCTGAGG CAGCTCACAC AGTGCCCAGA 
GACCAAGAGC CGTCCCTGTC GCCGCATGTA GACCCGGCCA AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098836.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954495.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OSe230354
Clone ID Related Accession (Same CDS sequence)	XM_021098836.1
Accession Version	XM_021098836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGGCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAC CAGCCTCTGC TGTTAATCCT 
AATGTAAAAT TAGGCAGTGT CAGACTGGAG GAGCCCACCT CCGCTCCTCC CACCGCTGAC 
CCCCTACATG CTGACTCTTT AAGAACACCA AGCCCTGAGG CAGCTCACAC AGTGCCCAGA 
GACCAAGAGC CGTCCCTGTC GCCGCATGTA GACCCGGCCA AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954495.1
CDS	68..5596
Translation	MRRLEGLCLRRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQKAEASSPGPYLSPETNPASPASAVNPNVKLGSVRLEEPTSAPPTADPLHADSLRTPSPEAAHTVPRDQEPSLSPHVDPAKVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098836.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098836.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGAAAGCAGA AGCCTCATCT 
CCAGGCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAC CAGCCTCTGC TGTTAATCCT 
AATGTAAAAT TAGGCAGTGT CAGACTGGAG GAGCCCACCT CCGCTCCTCC CACCGCTGAC 
CCCCTACATG CTGACTCTTT AAGAACACCA AGCCCTGAGG CAGCTCACAC AGTGCCCAGA 
GACCAAGAGC CGTCCCTGTC GCCGCATGTA GACCCGGCCA AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098843.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954502.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OSe230355
Clone ID Related Accession (Same CDS sequence)	XM_021098843.1
Accession Version	XM_021098843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954502.1
CDS	68..5416
Translation	MRRLEGLCLRRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098843.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098843.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTTGG 
TCTTTTGCTC TAAAATCATC CGACTCCTCG TCGGGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098845.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954504.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OSe230356
Clone ID Related Accession (Same CDS sequence)	XM_021098845.1
Accession Version	XM_021098845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
CCAGCCTCTG CTGTTAATCC TAATGTAAAA TTAGGCAGTG TCAGACTGGA GGAGCCCACC 
TCCGCTCCTC CCACCGCTGA CCCCCTACAT GCTGACTCTT TAAGAACACC AAGCCCTGAG 
GCAGCTCACA CAGTGCCCAG AGACCAAGAG CCGTCCCTGT CGCCGCATGT AGACCCGGCC 
AAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954504.1
CDS	461..5770
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQKAEASSPGPYLSPETNPASPASAVNPNVKLGSVRLEEPTSAPPTADPLHADSLRTPSPEAAHTVPRDQEPSLSPHVDPAKVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098845.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098845.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
CCAGCCTCTG CTGTTAATCC TAATGTAAAA TTAGGCAGTG TCAGACTGGA GGAGCCCACC 
TCCGCTCCTC CCACCGCTGA CCCCCTACAT GCTGACTCTT TAAGAACACC AAGCCCTGAG 
GCAGCTCACA CAGTGCCCAG AGACCAAGAG CCGTCCCTGT CGCCGCATGT AGACCCGGCC 
AAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098848.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954507.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OSe230357
Clone ID Related Accession (Same CDS sequence)	XM_021098848.1
Accession Version	XM_021098848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954507.1
CDS	68..5377
Translation	MRRLEGLCLRRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098848.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098848.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGCGT CCATGACTCC TGACGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098856.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954515.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OSe230358
Clone ID Related Accession (Same CDS sequence)	XM_021098856.1
Accession Version	XM_021098856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954515.1
CDS	68..5356
Translation	MRRLEGLCLRRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098856.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098856.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGAGG CCCTGGCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACGC CGTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATTA AAGGGAGACT ACACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACGGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC TGCAGAAGGG 
CAGCTGCAGG AAAATGACCG AGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CACGCTTTTG CTGTACAGCA ATTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACCATTCGC 
AGGAAGAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGACCCGGT GTCTGAGAAT 
GAAGAGGATA GTTATGAGGA AGAAGCACAC GACCCAAGAA GTGGCCGTGG GGGTCCTAAC 
CATAGGAGGA GTGAGAAGAG CTGGGCCAGG GAGAGAAGTG CCAGTAGGGA TAAGAGTCTG 
TCTCCAAGAT CCGATAGGCG GTCTGTGGCC TCCAGTCAAC CCACCAAACC CACCAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT 
GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTGA AGATAAATGG TACCGTGACA GAAAATATGT CATTGACGGA TGCAAAAACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACA 
CTGTTGAACG TCCCTGATCT TTCTGACAGT ATCCACTCTG CTAATGCTTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GGCGATCACA CGACAGGCCT 
CCCCGTCACA GCCGGTCACG GTCTCCTGAT CAGCGGTCAG AGCCTTCTGA CCATTCTAGA 
CACTCTCCAC AGCAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAGGAGGA GAGAACTACT 
AAACCCGGGG CAATCTCAAC TCCTGTAAAG CATGCAGATG ATCATACATC TAAAACAGTG 
GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCACCCA CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGACA ACCAGACGTG GATTTACCTG TCAGTCCATC TGATGGTATC 
CTACCTAATT CAACTCATGA AGATGGAATT CTTCGGCCCA GCATGAAGTT AGTAAAATTC 
AGAAAAGGTG ATAGTGTGGG TTTGCGACTG GCTGGTGGAA ATGACGTTGG AATATTTGTA 
GCTGGCGTTC TTGAAGACAG CCCTGCTGCC AAAGAAGGCT TAGAAGAGGG TGATCAGATT 
CTCAGGGTAA ATAATGTGGA CTTTACAAAT ATAATAAGAG AAGAAGCTGT CCTTTTCCTA 
CTTGACCTCC CGAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAC 
CGTCGCATTG TAGAGTCGGA TGTGGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTATTCCG TGTCGTGGAC 
ACTCTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGCAA AAATCATAAG 
GAGGTCGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTAGC CAGTGTCCAA 
TACACACTTC CAAAAACGGC AGGAGGAGAC CGCGCTGACT TTTGGAGATT CCGAGGCCTT 
CGGAGCTCCA AAAGAAATCT TCGCAAGAGC AGGGAAGACT TGTCAGCTCA GCCAGTCCAA 
ACCAAGTTTC CTGCATACGA GAGAGTTGTC CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATCTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAGA TCGCAAAAAG TGAACCTCGA GATGCTGGCA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTGGAT 
GTAACACCAA ATGCAGTTGA TCGTCTGAAT TATGCCCAGT GGTATCCAAT TGTAGTATTC 
CTTAACCCGG ATTCTAAGCA GGGTGTGAAA ACAATGCGAA TGAGGCTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAATT ATATGAACGG TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACCATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG AGCATTGAAA 
GAAGCAATTC AACAACAGCA GAACCAGTTG GTATGGGTTT CTGAGGGGAA GGCGGATGGC 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCCCCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TCAGAGAGGA CTCCTCTGGA 
ATGCATCATG AGAACCAAAC ATATCCTCCC TACTCACCAC AAGCGCAGCC ACAGCCAGTT 
CATAGCATAG ACTCCCCTGG ACTTAAAGCT GCCTCTCAAC AGGTGTATAG GAAGGACCCC 
TACCCCGAGG AAATGATGAG AGCCAACCAT GTCTTGAAGC AGCCAGCTGT CACTCACCCA 
GGGCAGAGGC CAGACAGAGA GCCGGGTCTG AGCTATGAGC CCCAGCCCCC GTACATGGAG 
AGACAGGCCA GCAGAGACCT GGAGCAGCCT GCGTACAGAT ATGAGTCCTC CAGCTATACT 
GACCAGCTTT CTCGAAACTA TGAACACCGT CTGCGCTGTG AAGACCACAC TCCTGCATAT 
GAGGAGAGGT GGTCCTACTA TGATGACAGG CAGTCCTACC AGCCTCGGCC TGCCCTGGAC 
AGCCAGCACC CTCGAGACCT TGACCCTAGA CAGCACCCTG AAGAGCCTTC AGAACGAGGG 
CATTTCCCAC GTTTCGAGGA GCCGACCCCA CCGTCCTATG AAAGCAGGCC TCGCTATGAC 
CAGCCACCCA GGACCTCTAC CCTGCGGCTG GAAGAGCCAT CCACTCCTGC CTATGATGCA 
CATAGTAGGT ACAGGCCTGA GGCTCAGCCC TATCCATCTG CCAGCCTGAA GGTCGCAGAG 
CCTAAGCAGT ATTTTGACCA GTATTCACGA AGTTATGAGC AGGTCCCGTC CCAAGGACTC 
GGCTCTAAAG CAGGCCATTA CGAGCCCCTC CACGGTGCTG CTGTCCCTCC TCTCACCCCT 
GCACCTCAGC ACAAGCCTGA AGTTCTCCCT TCAAGCACCA AACCGCTGCC TCCGCCCCCG 
GCTCTGGCTG AGGATGAAGA AGACCCAGCC ATGAAGCCGC AGTCAGTGCT CACCAGAGTA 
AAAATGTTCG AGAACAGAAG ATCTGCTTCC GTGGAGAGCA AGAAGGATGA CAGTCATCCT 
GCTGGTTTGA AGCCTCCAGA GGGAGCATCT AAGCCTGCAG GTGCTCCCAT CGTGGGTCCC 
AAAGGTCCTG CACAGAGTCA GCTCAGCGAA CATGACAGAA CACTGCACAG AATGCCAGAA 
CCTCAAAAAC CCCAAGTGAA GCCACCTGAA GATATTGTCC GGTCAAATCA TTATGATCCT 
GAGGAGGATG AAGAGTATTA CCGAAAACAG CTCTCATACT TTGATCGAAG AAGTTTTGAG 
AACAAGCCTT CTGCACACGT GCCTGCCAGC CATCTCTCAG AGCCTGCCAA GTCAGTTCAT 
TCTCAGAATC AGCCACATTT TTCAAGTTAT CCTTCCAAGG TAAAGTCTGC TGAGGCTGAA 
GCTGTAGACA GGTCGTTTGG GGAGAAGCGC TATGACCCAC TGCAGGCAAC ACCCCCTCCT 
CCGCCATTGC CCACCCAGTA CAGCCAGCCC TCTCAGCCCG GCCCCAGCAC TGCCCTTGGC 
CTCCACGTAC ATGCCAAGGG GGCGCACGGC GAAGGTAATT CAGTGTCATT GGATTTTCAA 
AACTCATTAG TGTCTAAACC AGACCCACCT CCTGCTCAGA ATAAGCCAGC AACTTACAGA 
CCACCAAACC GAGAAGATAC TGCTCAGTCT GCTTTCTATC CCCAGAAAAG TTTCCCAGAA 
AAAGGTCCAG CCAATGGGGC CGAACAGACT CAGAAAACGA TCACTCCAGC ATACAATCGA 
TTCACACCAA AACCATACAC AAGTTCTGCT CGGCCATTTG AACGCAAGTT TGAAAGCCCT 
AAGTTCAATC ACAATCTTCT GCCAAGTGAA ATCATACATA AACCTGAATT GTCTTCCAAA 
GCCCCTGCTT CTCCAAAAAC TCTTGTGAAA ACACATGGTT CAGTGCCGCC TCCTGAGTTT 
GATAGTGGCG TTGACACGTT CTCTGTCCAT GCAGATAAGC CTAAATATCA AGTAAATAAC 
ATCAGCACAG TGCCTAAAGC TGTTCCTGTG AGTCCTTCAG CTGTAGAAGA TGATGAAGAT 
GAGGATGGTC ACACCGTGGT GGCCACAGCC CGAGGCGTGT TTAACAGCAA CGGCGGGGTG 
TTGAGCTCCA TAGAAACTGG CGTTAGCATA ATTATCCCAC AGGGAGCTAT TCCTGAGGGA 
ATTGAGCAGG AAATCTATTT CAAGGTCTGC CGAGACAACA GCATCCTCCC ACCTTTAGAT 
AAGGAGAAAG GTGAAACCCT GCTGAGCCCC TTAGTGATGT GCGGGCCCCA TGGTCTCAAG 
TTCCTGAAGC CCGTGGAGCT GCGCTTACCA CACTGTGATC CTAAAACCTG GCAAAACAAG 
TGTCTTCCTG GAGATCCAAA TTATCTTGTT GGAGCAAACT GTGTCTCTGT CCTGATTGAC 
CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098860.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954519.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X8, mRNA.

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CloneID	OSe230359
Clone ID Related Accession (Same CDS sequence)	XM_021098860.1
Accession Version	XM_021098860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CAGCTATATG GGAACAACAC ACGGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATAGTGA TTTCAGATGT GCTGAAAGGA GGACCTGCAG AAGGGCAGCT GCAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAATG TTGAACACGC TTTTGCTGTA 
CAGCAATTAA GGAAAAGTGG GAAAAATGCA AAAATTACCA TTCGCAGGAA GAAGAAAGTT 
CAGATACCAG TAAGTCGTCC TGATCCTGAC CCGGTGTCTG AGAATGAAGA GGATAGTTAT 
GAGGAAGAAG CACACGACCC AAGAAGTGGC CGTGGGGGTC CTAACCATAG GAGGAGTGAG 
AAGAGCTGGG CCAGGGAGAG AAGTGCCAGT AGGGATAAGA GTCTGTCTCC AAGATCCGAT 
AGGCGGTCTG TGGCCTCCAG TCAACCCACC AAACCCACCA AAGTCACACT GGTGAAATCC 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA 
AATGGTACCG TGACAGAAAA TATGTCATTG ACGGATGCAA AAACATTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACACTGTT GAACGTCCCT 
GATCTTTCTG ACAGTATCCA CTCTGCTAAT GCTTCTGAGA GAGATGACAT TTCAGAAATT 
CAGTCACTGG CATCAGATCA TTCTGGGCGA TCACACGACA GGCCTCCCCG TCACAGCCGG 
TCACGGTCTC CTGATCAGCG GTCAGAGCCT TCTGACCATT CTAGACACTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAGGAGAGAA CTACTAAACC CGGGGCAATC 
TCAACTCCTG TAAAGCATGC AGATGATCAT ACATCTAAAA CAGTGGAAGA AGTTATAGTT 
GAAAGAAATG AGAAACAAGC ACCCACTCTT CCAGAACCAA AGCCTGTGTA TGCCCAAGTT 
GGACAACCAG ACGTGGATTT ACCTGTCAGT CCATCTGATG GTATCCTACC TAATTCAACT 
CATGAAGATG GAATTCTTCG GCCCAGCATG AAGTTAGTAA AATTCAGAAA AGGTGATAGT 
GTGGGTTTGC GACTGGCTGG TGGAAATGAC GTTGGAATAT TTGTAGCTGG CGTTCTTGAA 
GACAGCCCTG CTGCCAAAGA AGGCTTAGAA GAGGGTGATC AGATTCTCAG GGTAAATAAT 
GTGGACTTTA CAAATATAAT AAGAGAAGAA GCTGTCCTTT TCCTACTTGA CCTCCCGAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTACCGTCG CATTGTAGAG 
TCGGATGTGG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTA TTCCGTGTCG TGGACACTCT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT CGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TCCAATACAC ACTTCCAAAA 
ACGGCAGGAG GAGACCGCGC TGACTTTTGG AGATTCCGAG GCCTTCGGAG CTCCAAAAGA 
AATCTTCGCA AGAGCAGGGA AGACTTGTCA GCTCAGCCAG TCCAAACCAA GTTTCCTGCA 
TACGAGAGAG TTGTCCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATCGCA 
AAAAGTGAAC CTCGAGATGC TGGCACTGAC CAACGTAGCT CTGGCATTAT TCGCCTTCAT 
ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TGGATGTAAC ACCAAATGCA 
GTTGATCGTC TGAATTATGC CCAGTGGTAT CCAATTGTAG TATTCCTTAA CCCGGATTCT 
AAGCAGGGTG TGAAAACAAT GCGAATGAGG CTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAATTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACC 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGAGCAT TGAAAGAAGC AATTCAACAA 
CAGCAGAACC AGTTGGTATG GGTTTCTGAG GGGAAGGCGG ATGGCGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTCAGA GAGGACTCCT CTGGAATGCA TCATGAGAAC 
CAAACATATC CTCCCTACTC ACCACAAGCG CAGCCACAGC CAGTTCATAG CATAGACTCC 
CCTGGACTTA AAGCTGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGG CCCTTACCTT 
TCGCCTGAAA CAAACCCAGC ATCACCAGCC TCTGCTGTTA ATCCTAATGT AAAATTAGGC 
AGTGTCAGAC TGGAGGAGCC CACCTCCGCT CCTCCCACCG CTGACCCCCT ACATGCTGAC 
TCTTTAAGAA CACCAAGCCC TGAGGCAGCT CACACAGTGC CCAGAGACCA AGAGCCGTCC 
CTGTCGCCGC ATGTAGACCC GGCCAAGGTG TATAGGAAGG ACCCCTACCC CGAGGAAATG 
ATGAGAGCCA ACCATGTCTT GAAGCAGCCA GCTGTCACTC ACCCAGGGCA GAGGCCAGAC 
AGAGAGCCGG GTCTGAGCTA TGAGCCCCAG CCCCCGTACA TGGAGAGACA GGCCAGCAGA 
GACCTGGAGC AGCCTGCGTA CAGATATGAG TCCTCCAGCT ATACTGACCA GCTTTCTCGA 
AACTATGAAC ACCGTCTGCG CTGTGAAGAC CACACTCCTG CATATGAGGA GAGGTGGTCC 
TACTATGATG ACAGGCAGTC CTACCAGCCT CGGCCTGCCC TGGACAGCCA GCACCCTCGA 
GACCTTGACC CTAGACAGCA CCCTGAAGAG CCTTCAGAAC GAGGGCATTT CCCACGTTTC 
GAGGAGCCGA CCCCACCGTC CTATGAAAGC AGGCCTCGCT ATGACCAGCC ACCCAGGACC 
TCTACCCTGC GGCTGGAAGA GCCATCCACT CCTGCCTATG ATGCACATAG TAGGTACAGG 
CCTGAGGCTC AGCCCTATCC ATCTGCCAGC CTGAAGGTCG CAGAGCCTAA GCAGTATTTT 
GACCAGTATT CACGAAGTTA TGAGCAGGTC CCGTCCCAAG GACTCGGCTC TAAAGCAGGC 
CATTACGAGC CCCTCCACGG TGCTGCTGTC CCTCCTCTCA CCCCTGCACC TCAGCACAAG 
CCTGAAGTTC TCCCTTCAAG CACCAAACCG CTGCCTCCGC CCCCGGCTCT GGCTGAGGAT 
GAAGAAGACC CAGCCATGAA GCCGCAGTCA GTGCTCACCA GAGTAAAAAT GTTCGAGAAC 
AGAAGATCTG CTTCCGTGGA GAGCAAGAAG GATGACAGTC ATCCTGCTGG TTTGAAGCCT 
CCAGAGGGAG CATCTAAGCC TGCAGGTGCT CCCATCGTGG GTCCCAAAGG TCCTGCACAG 
AGTCAGCTCA GCGAACATGA CAGAACACTG CACAGAATGC CAGAACCTCA AAAACCCCAA 
GTGAAGCCAC CTGAAGATAT TGTCCGGTCA AATCATTATG ATCCTGAGGA GGATGAAGAG 
TATTACCGAA AACAGCTCTC ATACTTTGAT CGAAGAAGTT TTGAGAACAA GCCTTCTGCA 
CACGTGCCTG CCAGCCATCT CTCAGAGCCT GCCAAGTCAG TTCATTCTCA GAATCAGCCA 
CATTTTTCAA GTTATCCTTC CAAGGTAAAG TCTGCTGAGG CTGAAGCTGT AGACAGGTCG 
TTTGGGGAGA AGCGCTATGA CCCACTGCAG GCAACACCCC CTCCTCCGCC ATTGCCCACC 
CAGTACAGCC AGCCCTCTCA GCCCGGCCCC AGCACTGCCC TTGGCCTCCA CGTACATGCC 
AAGGGGGCGC ACGGCGAAGG TAATTCAGTG TCATTGGATT TTCAAAACTC ATTAGTGTCT 
AAACCAGACC CACCTCCTGC TCAGAATAAG CCAGCAACTT ACAGACCACC AAACCGAGAA 
GATACTGCTC AGTCTGCTTT CTATCCCCAG AAAAGTTTCC CAGAAAAAGG TCCAGCCAAT 
GGGGCCGAAC AGACTCAGAA AACGATCACT CCAGCATACA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAGTT CAATCACAAT 
CTTCTGCCAA GTGAAATCAT ACATAAACCT GAATTGTCTT CCAAAGCCCC TGCTTCTCCA 
AAAACTCTTG TGAAAACACA TGGTTCAGTG CCGCCTCCTG AGTTTGATAG TGGCGTTGAC 
ACGTTCTCTG TCCATGCAGA TAAGCCTAAA TATCAAGTAA ATAACATCAG CACAGTGCCT 
AAAGCTGTTC CTGTGAGTCC TTCAGCTGTA GAAGATGATG AAGATGAGGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAACGGCG GGGTGTTGAG CTCCATAGAA 
ACTGGCGTTA GCATAATTAT CCCACAGGGA GCTATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGTGAA 
ACCCTGCTGA GCCCCTTAGT GATGTGCGGG CCCCATGGTC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTC TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954519.1
CDS	355..5628
Translation	MEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQKAEASSPGPYLSPETNPASPASAVNPNVKLGSVRLEEPTSAPPTADPLHADSLRTPSPEAAHTVPRDQEPSLSPHVDPAKVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098860.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098860.1

ORF Insert Sequence:

ATGGAGGAAA CAGCTATATG GGAACAACAC ACGGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATAGTGA TTTCAGATGT GCTGAAAGGA GGACCTGCAG AAGGGCAGCT GCAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAATG TTGAACACGC TTTTGCTGTA 
CAGCAATTAA GGAAAAGTGG GAAAAATGCA AAAATTACCA TTCGCAGGAA GAAGAAAGTT 
CAGATACCAG TAAGTCGTCC TGATCCTGAC CCGGTGTCTG AGAATGAAGA GGATAGTTAT 
GAGGAAGAAG CACACGACCC AAGAAGTGGC CGTGGGGGTC CTAACCATAG GAGGAGTGAG 
AAGAGCTGGG CCAGGGAGAG AAGTGCCAGT AGGGATAAGA GTCTGTCTCC AAGATCCGAT 
AGGCGGTCTG TGGCCTCCAG TCAACCCACC AAACCCACCA AAGTCACACT GGTGAAATCC 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA 
AATGGTACCG TGACAGAAAA TATGTCATTG ACGGATGCAA AAACATTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACACTGTT GAACGTCCCT 
GATCTTTCTG ACAGTATCCA CTCTGCTAAT GCTTCTGAGA GAGATGACAT TTCAGAAATT 
CAGTCACTGG CATCAGATCA TTCTGGGCGA TCACACGACA GGCCTCCCCG TCACAGCCGG 
TCACGGTCTC CTGATCAGCG GTCAGAGCCT TCTGACCATT CTAGACACTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAGGAGAGAA CTACTAAACC CGGGGCAATC 
TCAACTCCTG TAAAGCATGC AGATGATCAT ACATCTAAAA CAGTGGAAGA AGTTATAGTT 
GAAAGAAATG AGAAACAAGC ACCCACTCTT CCAGAACCAA AGCCTGTGTA TGCCCAAGTT 
GGACAACCAG ACGTGGATTT ACCTGTCAGT CCATCTGATG GTATCCTACC TAATTCAACT 
CATGAAGATG GAATTCTTCG GCCCAGCATG AAGTTAGTAA AATTCAGAAA AGGTGATAGT 
GTGGGTTTGC GACTGGCTGG TGGAAATGAC GTTGGAATAT TTGTAGCTGG CGTTCTTGAA 
GACAGCCCTG CTGCCAAAGA AGGCTTAGAA GAGGGTGATC AGATTCTCAG GGTAAATAAT 
GTGGACTTTA CAAATATAAT AAGAGAAGAA GCTGTCCTTT TCCTACTTGA CCTCCCGAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTACCGTCG CATTGTAGAG 
TCGGATGTGG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTA TTCCGTGTCG TGGACACTCT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT CGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TCCAATACAC ACTTCCAAAA 
ACGGCAGGAG GAGACCGCGC TGACTTTTGG AGATTCCGAG GCCTTCGGAG CTCCAAAAGA 
AATCTTCGCA AGAGCAGGGA AGACTTGTCA GCTCAGCCAG TCCAAACCAA GTTTCCTGCA 
TACGAGAGAG TTGTCCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATCGCA 
AAAAGTGAAC CTCGAGATGC TGGCACTGAC CAACGTAGCT CTGGCATTAT TCGCCTTCAT 
ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TGGATGTAAC ACCAAATGCA 
GTTGATCGTC TGAATTATGC CCAGTGGTAT CCAATTGTAG TATTCCTTAA CCCGGATTCT 
AAGCAGGGTG TGAAAACAAT GCGAATGAGG CTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAATTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACC 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGAGCAT TGAAAGAAGC AATTCAACAA 
CAGCAGAACC AGTTGGTATG GGTTTCTGAG GGGAAGGCGG ATGGCGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTCAGA GAGGACTCCT CTGGAATGCA TCATGAGAAC 
CAAACATATC CTCCCTACTC ACCACAAGCG CAGCCACAGC CAGTTCATAG CATAGACTCC 
CCTGGACTTA AAGCTGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGG CCCTTACCTT 
TCGCCTGAAA CAAACCCAGC ATCACCAGCC TCTGCTGTTA ATCCTAATGT AAAATTAGGC 
AGTGTCAGAC TGGAGGAGCC CACCTCCGCT CCTCCCACCG CTGACCCCCT ACATGCTGAC 
TCTTTAAGAA CACCAAGCCC TGAGGCAGCT CACACAGTGC CCAGAGACCA AGAGCCGTCC 
CTGTCGCCGC ATGTAGACCC GGCCAAGGTG TATAGGAAGG ACCCCTACCC CGAGGAAATG 
ATGAGAGCCA ACCATGTCTT GAAGCAGCCA GCTGTCACTC ACCCAGGGCA GAGGCCAGAC 
AGAGAGCCGG GTCTGAGCTA TGAGCCCCAG CCCCCGTACA TGGAGAGACA GGCCAGCAGA 
GACCTGGAGC AGCCTGCGTA CAGATATGAG TCCTCCAGCT ATACTGACCA GCTTTCTCGA 
AACTATGAAC ACCGTCTGCG CTGTGAAGAC CACACTCCTG CATATGAGGA GAGGTGGTCC 
TACTATGATG ACAGGCAGTC CTACCAGCCT CGGCCTGCCC TGGACAGCCA GCACCCTCGA 
GACCTTGACC CTAGACAGCA CCCTGAAGAG CCTTCAGAAC GAGGGCATTT CCCACGTTTC 
GAGGAGCCGA CCCCACCGTC CTATGAAAGC AGGCCTCGCT ATGACCAGCC ACCCAGGACC 
TCTACCCTGC GGCTGGAAGA GCCATCCACT CCTGCCTATG ATGCACATAG TAGGTACAGG 
CCTGAGGCTC AGCCCTATCC ATCTGCCAGC CTGAAGGTCG CAGAGCCTAA GCAGTATTTT 
GACCAGTATT CACGAAGTTA TGAGCAGGTC CCGTCCCAAG GACTCGGCTC TAAAGCAGGC 
CATTACGAGC CCCTCCACGG TGCTGCTGTC CCTCCTCTCA CCCCTGCACC TCAGCACAAG 
CCTGAAGTTC TCCCTTCAAG CACCAAACCG CTGCCTCCGC CCCCGGCTCT GGCTGAGGAT 
GAAGAAGACC CAGCCATGAA GCCGCAGTCA GTGCTCACCA GAGTAAAAAT GTTCGAGAAC 
AGAAGATCTG CTTCCGTGGA GAGCAAGAAG GATGACAGTC ATCCTGCTGG TTTGAAGCCT 
CCAGAGGGAG CATCTAAGCC TGCAGGTGCT CCCATCGTGG GTCCCAAAGG TCCTGCACAG 
AGTCAGCTCA GCGAACATGA CAGAACACTG CACAGAATGC CAGAACCTCA AAAACCCCAA 
GTGAAGCCAC CTGAAGATAT TGTCCGGTCA AATCATTATG ATCCTGAGGA GGATGAAGAG 
TATTACCGAA AACAGCTCTC ATACTTTGAT CGAAGAAGTT TTGAGAACAA GCCTTCTGCA 
CACGTGCCTG CCAGCCATCT CTCAGAGCCT GCCAAGTCAG TTCATTCTCA GAATCAGCCA 
CATTTTTCAA GTTATCCTTC CAAGGTAAAG TCTGCTGAGG CTGAAGCTGT AGACAGGTCG 
TTTGGGGAGA AGCGCTATGA CCCACTGCAG GCAACACCCC CTCCTCCGCC ATTGCCCACC 
CAGTACAGCC AGCCCTCTCA GCCCGGCCCC AGCACTGCCC TTGGCCTCCA CGTACATGCC 
AAGGGGGCGC ACGGCGAAGG TAATTCAGTG TCATTGGATT TTCAAAACTC ATTAGTGTCT 
AAACCAGACC CACCTCCTGC TCAGAATAAG CCAGCAACTT ACAGACCACC AAACCGAGAA 
GATACTGCTC AGTCTGCTTT CTATCCCCAG AAAAGTTTCC CAGAAAAAGG TCCAGCCAAT 
GGGGCCGAAC AGACTCAGAA AACGATCACT CCAGCATACA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAGTT CAATCACAAT 
CTTCTGCCAA GTGAAATCAT ACATAAACCT GAATTGTCTT CCAAAGCCCC TGCTTCTCCA 
AAAACTCTTG TGAAAACACA TGGTTCAGTG CCGCCTCCTG AGTTTGATAG TGGCGTTGAC 
ACGTTCTCTG TCCATGCAGA TAAGCCTAAA TATCAAGTAA ATAACATCAG CACAGTGCCT 
AAAGCTGTTC CTGTGAGTCC TTCAGCTGTA GAAGATGATG AAGATGAGGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAACGGCG GGGTGTTGAG CTCCATAGAA 
ACTGGCGTTA GCATAATTAT CCCACAGGGA GCTATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGTGAA 
ACCCTGCTGA GCCCCTTAGT GATGTGCGGG CCCCATGGTC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTC TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098866.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954525.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X9, mRNA.

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CloneID	OSe230360
Clone ID Related Accession (Same CDS sequence)	XM_021098866.1
Accession Version	XM_021098866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
CCAGCCTCTG CTGTTAATCC TAATGTAAAA TTAGGCAGTG TCAGACTGGA GGAGCCCACC 
TCCGCTCCTC CCACCGCTGA CCCCCTACAT GCTGACTCTT TAAGAACACC AAGCCCTGAG 
GCAGCTCACA CAGTGCCCAG AGACCAAGAG CCGTCCCTGT CGCCGCATGT AGACCCGGCC 
AAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTCTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954525.1
CDS	440..5710
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQKAEASSPGPYLSPETNPASPASAVNPNVKLGSVRLEEPTSAPPTADPLHADSLRTPSPEAAHTVPRDQEPSLSPHVDPAKVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098866.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098866.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
CCAGCCTCTG CTGTTAATCC TAATGTAAAA TTAGGCAGTG TCAGACTGGA GGAGCCCACC 
TCCGCTCCTC CCACCGCTGA CCCCCTACAT GCTGACTCTT TAAGAACACC AAGCCCTGAG 
GCAGCTCACA CAGTGCCCAG AGACCAAGAG CCGTCCCTGT CGCCGCATGT AGACCCGGCC 
AAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTCTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_003480423.4 ) cDNA ORF clone, Sus scrofa(pig) -> XP_003480471.3 Sus scrofa tight junction protein 1 (TJP1), transcript variant X10, mRNA.

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CloneID	OSe230361
Clone ID Related Accession (Same CDS sequence)	XM_003480423.4
Accession Version	XM_003480423.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_003480423.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
CCAGCCTCTG CTGTTAATCC TAATGTAAAA TTAGGCAGTG TCAGACTGGA GGAGCCCACC 
TCCGCTCCTC CCACCGCTGA CCCCCTACAT GCTGACTCTT TAAGAACACC AAGCCCTGAG 
GCAGCTCACA CAGTGCCCAG AGACCAAGAG CCGTCCCTGT CGCCGCATGT AGACCCGGCC 
AAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003480471.3
CDS	438..5687
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQKAEASSPGPYLSPETNPASPASAVNPNVKLGSVRLEEPTSAPPTADPLHADSLRTPSPEAAHTVPRDQEPSLSPHVDPAKVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_003480423.4
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003480423.4

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
CCAGCCTCTG CTGTTAATCC TAATGTAAAA TTAGGCAGTG TCAGACTGGA GGAGCCCACC 
TCCGCTCCTC CCACCGCTGA CCCCCTACAT GCTGACTCTT TAAGAACACC AAGCCCTGAG 
GCAGCTCACA CAGTGCCCAG AGACCAAGAG CCGTCCCTGT CGCCGCATGT AGACCCGGCC 
AAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098878.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954537.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OSe230362
Clone ID Related Accession (Same CDS sequence)	XM_021098878.1
Accession Version	XM_021098878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954537.1
CDS	445..5514
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098878.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098878.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098883.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954542.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OSe230363
Clone ID Related Accession (Same CDS sequence)	XM_021098883.1
Accession Version	XM_021098883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CAGCTATATG GGAACAACAC ACGGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATAGTGA TTTCAGATGT GCTGAAAGGA GGACCTGCAG AAGGGCAGCT GCAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAATG TTGAACACGC TTTTGCTGTA 
CAGCAATTAA GGAAAAGTGG GAAAAATGCA AAAATTACCA TTCGCAGGAA GAAGAAAGTT 
CAGATACCAG TAAGTCGTCC TGATCCTGAC CCGGTGTCTG AGAATGAAGA GGATAGTTAT 
GAGGAAGAAG CACACGACCC AAGAAGTGGC CGTGGGGGTC CTAACCATAG GAGGAGTGAG 
AAGAGCTGGG CCAGGGAGAG AAGTGCCAGT AGGGATAAGA GTCTGTCTCC AAGATCCGAT 
AGGCGGTCTG TGGCCTCCAG TCAACCCACC AAACCCACCA AAGTCACACT GGTGAAATCC 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA 
AATGGTACCG TGACAGAAAA TATGTCATTG ACGGATGCAA AAACATTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACACTGTT GAACGTCCCT 
GATCTTTCTG ACAGTATCCA CTCTGCTAAT GCTTCTGAGA GAGATGACAT TTCAGAAATT 
CAGTCACTGG CATCAGATCA TTCTGGGCGA TCACACGACA GGCCTCCCCG TCACAGCCGG 
TCACGGTCTC CTGATCAGCG GTCAGAGCCT TCTGACCATT CTAGACACTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAGGAGAGAA CTACTAAACC CGGGGCAATC 
TCAACTCCTG TAAAGCATGC AGATGATCAT ACATCTAAAA CAGTGGAAGA AGTTATAGTT 
GAAAGAAATG AGAAACAAGC ACCCACTCTT CCAGAACCAA AGCCTGTGTA TGCCCAAGTT 
GGACAACCAG ACGTGGATTT ACCTGTCAGT CCATCTGATG GTATCCTACC TAATTCAACT 
CATGAAGATG GAATTCTTCG GCCCAGCATG AAGTTAGTAA AATTCAGAAA AGGTGATAGT 
GTGGGTTTGC GACTGGCTGG TGGAAATGAC GTTGGAATAT TTGTAGCTGG CGTTCTTGAA 
GACAGCCCTG CTGCCAAAGA AGGCTTAGAA GAGGGTGATC AGATTCTCAG GGTAAATAAT 
GTGGACTTTA CAAATATAAT AAGAGAAGAA GCTGTCCTTT TCCTACTTGA CCTCCCGAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTACCGTCG CATTGTAGAG 
TCGGATGTGG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTA TTCCGTGTCG TGGACACTCT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT CGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TCCAATACAC ACTTCCAAAA 
ACGGCAGGAG GAGACCGCGC TGACTTTTGG AGATTCCGAG GCCTTCGGAG CTCCAAAAGA 
AATCTTCGCA AGAGCAGGGA AGACTTGTCA GCTCAGCCAG TCCAAACCAA GTTTCCTGCA 
TACGAGAGAG TTGTCCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATCGCA 
AAAAGTGAAC CTCGAGATGC TGGCACTGAC CAACGTAGCT CTGGCATTAT TCGCCTTCAT 
ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TGGATGTAAC ACCAAATGCA 
GTTGATCGTC TGAATTATGC CCAGTGGTAT CCAATTGTAG TATTCCTTAA CCCGGATTCT 
AAGCAGGGTG TGAAAACAAT GCGAATGAGG CTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAATTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACC 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGAGCAT TGAAAGAAGC AATTCAACAA 
CAGCAGAACC AGTTGGTATG GGTTTCTGAG GGGAAGGCGG ATGGCGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTCAGA GAGGACTCCT CTGGAATGCA TCATGAGAAC 
CAAACATATC CTCCCTACTC ACCACAAGCG CAGCCACAGC CAGTTCATAG CATAGACTCC 
CCTGGACTTA AAGCTGCCTC TCAACAGGTG TATAGGAAGG ACCCCTACCC CGAGGAAATG 
ATGAGAGCCA ACCATGTCTT GAAGCAGCCA GCTGTCACTC ACCCAGGGCA GAGGCCAGAC 
AGAGAGCCGG GTCTGAGCTA TGAGCCCCAG CCCCCGTACA TGGAGAGACA GGCCAGCAGA 
GACCTGGAGC AGCCTGCGTA CAGATATGAG TCCTCCAGCT ATACTGACCA GCTTTCTCGA 
AACTATGAAC ACCGTCTGCG CTGTGAAGAC CACACTCCTG CATATGAGGA GAGGTGGTCC 
TACTATGATG ACAGGCAGTC CTACCAGCCT CGGCCTGCCC TGGACAGCCA GCACCCTCGA 
GACCTTGACC CTAGACAGCA CCCTGAAGAG CCTTCAGAAC GAGGGCATTT CCCACGTTTC 
GAGGAGCCGA CCCCACCGTC CTATGAAAGC AGGCCTCGCT ATGACCAGCC ACCCAGGACC 
TCTACCCTGC GGCTGGAAGA GCCATCCACT CCTGCCTATG ATGCACATAG TAGGTACAGG 
CCTGAGGCTC AGCCCTATCC ATCTGCCAGC CTGAAGGTCG CAGAGCCTAA GCAGTATTTT 
GACCAGTATT CACGAAGTTA TGAGCAGGTC CCGTCCCAAG GACTCGGCTC TAAAGCAGGC 
CATTACGAGC CCCTCCACGG TGCTGCTGTC CCTCCTCTCA CCCCTGCACC TCAGCACAAG 
CCTGAAGTTC TCCCTTCAAG CACCAAACCG CTGCCTCCGC CCCCGGCTCT GGCTGAGGAT 
GAAGAAGACC CAGCCATGAA GCCGCAGTCA GTGCTCACCA GAGTAAAAAT GTTCGAGAAC 
AGAAGATCTG CTTCCGTGGA GAGCAAGAAG GATGACAGTC ATCCTGCTGG TTTGAAGCCT 
CCAGAGGGAG CATCTAAGCC TGCAGGTGCT CCCATCGTGG GTCCCAAAGG TCCTGCACAG 
AGTCAGCTCA GCGAACATGA CAGAACACTG CACAGAATGC CAGAACCTCA AAAACCCCAA 
GTGAAGCCAC CTGAAGATAT TGTCCGGTCA AATCATTATG ATCCTGAGGA GGATGAAGAG 
TATTACCGAA AACAGCTCTC ATACTTTGAT CGAAGAAGTT TTGAGAACAA GCCTTCTGCA 
CACGTGCCTG CCAGCCATCT CTCAGAGCCT GCCAAGTCAG TTCATTCTCA GAATCAGCCA 
CATTTTTCAA GTTATCCTTC CAAGGTAAAG TCTGCTGAGG CTGAAGCTGT AGACAGGTCG 
TTTGGGGAGA AGCGCTATGA CCCACTGCAG GCAACACCCC CTCCTCCGCC ATTGCCCACC 
CAGTACAGCC AGCCCTCTCA GCCCGGCCCC AGCACTGCCC TTGGCCTCCA CGTACATGCC 
AAGGGGGCGC ACGGCGAAGG TAATTCAGTG TCATTGGATT TTCAAAACTC ATTAGTGTCT 
AAACCAGACC CACCTCCTGC TCAGAATAAG CCAGCAACTT ACAGACCACC AAACCGAGAA 
GATACTGCTC AGTCTGCTTT CTATCCCCAG AAAAGTTTCC CAGAAAAAGG TCCAGCCAAT 
GGGGCCGAAC AGACTCAGAA AACGATCACT CCAGCATACA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAGTT CAATCACAAT 
CTTCTGCCAA GTGAAATCAT ACATAAACCT GAATTGTCTT CCAAAGCCCC TGCTTCTCCA 
AAAACTCTTG TGAAAACACA TGGTTCAGTG CCGCCTCCTG AGTTTGATAG TGGCGTTGAC 
ACGTTCTCTG TCCATGCAGA TAAGCCTAAA TATCAAGTAA ATAACATCAG CACAGTGCCT 
AAAGCTGTTC CTGTGAGTCC TTCAGCTGTA GAAGATGATG AAGATGAGGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAACGGCG GGGTGTTGAG CTCCATAGAA 
ACTGGCGTTA GCATAATTAT CCCACAGGGA GCTATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGTGAA 
ACCCTGCTGA GCCCCTTAGT GATGTGCGGG CCCCATGGTC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTC TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954542.1
CDS	353..5386
Translation	MEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098883.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098883.1

ORF Insert Sequence:

ATGGAGGAAA CAGCTATATG GGAACAACAC ACGGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATAGTGA TTTCAGATGT GCTGAAAGGA GGACCTGCAG AAGGGCAGCT GCAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAATG TTGAACACGC TTTTGCTGTA 
CAGCAATTAA GGAAAAGTGG GAAAAATGCA AAAATTACCA TTCGCAGGAA GAAGAAAGTT 
CAGATACCAG TAAGTCGTCC TGATCCTGAC CCGGTGTCTG AGAATGAAGA GGATAGTTAT 
GAGGAAGAAG CACACGACCC AAGAAGTGGC CGTGGGGGTC CTAACCATAG GAGGAGTGAG 
AAGAGCTGGG CCAGGGAGAG AAGTGCCAGT AGGGATAAGA GTCTGTCTCC AAGATCCGAT 
AGGCGGTCTG TGGCCTCCAG TCAACCCACC AAACCCACCA AAGTCACACT GGTGAAATCC 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA 
AATGGTACCG TGACAGAAAA TATGTCATTG ACGGATGCAA AAACATTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACACTGTT GAACGTCCCT 
GATCTTTCTG ACAGTATCCA CTCTGCTAAT GCTTCTGAGA GAGATGACAT TTCAGAAATT 
CAGTCACTGG CATCAGATCA TTCTGGGCGA TCACACGACA GGCCTCCCCG TCACAGCCGG 
TCACGGTCTC CTGATCAGCG GTCAGAGCCT TCTGACCATT CTAGACACTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAGGAGAGAA CTACTAAACC CGGGGCAATC 
TCAACTCCTG TAAAGCATGC AGATGATCAT ACATCTAAAA CAGTGGAAGA AGTTATAGTT 
GAAAGAAATG AGAAACAAGC ACCCACTCTT CCAGAACCAA AGCCTGTGTA TGCCCAAGTT 
GGACAACCAG ACGTGGATTT ACCTGTCAGT CCATCTGATG GTATCCTACC TAATTCAACT 
CATGAAGATG GAATTCTTCG GCCCAGCATG AAGTTAGTAA AATTCAGAAA AGGTGATAGT 
GTGGGTTTGC GACTGGCTGG TGGAAATGAC GTTGGAATAT TTGTAGCTGG CGTTCTTGAA 
GACAGCCCTG CTGCCAAAGA AGGCTTAGAA GAGGGTGATC AGATTCTCAG GGTAAATAAT 
GTGGACTTTA CAAATATAAT AAGAGAAGAA GCTGTCCTTT TCCTACTTGA CCTCCCGAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTACCGTCG CATTGTAGAG 
TCGGATGTGG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTA TTCCGTGTCG TGGACACTCT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT CGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TCCAATACAC ACTTCCAAAA 
ACGGCAGGAG GAGACCGCGC TGACTTTTGG AGATTCCGAG GCCTTCGGAG CTCCAAAAGA 
AATCTTCGCA AGAGCAGGGA AGACTTGTCA GCTCAGCCAG TCCAAACCAA GTTTCCTGCA 
TACGAGAGAG TTGTCCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATCGCA 
AAAAGTGAAC CTCGAGATGC TGGCACTGAC CAACGTAGCT CTGGCATTAT TCGCCTTCAT 
ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TGGATGTAAC ACCAAATGCA 
GTTGATCGTC TGAATTATGC CCAGTGGTAT CCAATTGTAG TATTCCTTAA CCCGGATTCT 
AAGCAGGGTG TGAAAACAAT GCGAATGAGG CTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAATTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACC 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGAGCAT TGAAAGAAGC AATTCAACAA 
CAGCAGAACC AGTTGGTATG GGTTTCTGAG GGGAAGGCGG ATGGCGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTCAGA GAGGACTCCT CTGGAATGCA TCATGAGAAC 
CAAACATATC CTCCCTACTC ACCACAAGCG CAGCCACAGC CAGTTCATAG CATAGACTCC 
CCTGGACTTA AAGCTGCCTC TCAACAGGTG TATAGGAAGG ACCCCTACCC CGAGGAAATG 
ATGAGAGCCA ACCATGTCTT GAAGCAGCCA GCTGTCACTC ACCCAGGGCA GAGGCCAGAC 
AGAGAGCCGG GTCTGAGCTA TGAGCCCCAG CCCCCGTACA TGGAGAGACA GGCCAGCAGA 
GACCTGGAGC AGCCTGCGTA CAGATATGAG TCCTCCAGCT ATACTGACCA GCTTTCTCGA 
AACTATGAAC ACCGTCTGCG CTGTGAAGAC CACACTCCTG CATATGAGGA GAGGTGGTCC 
TACTATGATG ACAGGCAGTC CTACCAGCCT CGGCCTGCCC TGGACAGCCA GCACCCTCGA 
GACCTTGACC CTAGACAGCA CCCTGAAGAG CCTTCAGAAC GAGGGCATTT CCCACGTTTC 
GAGGAGCCGA CCCCACCGTC CTATGAAAGC AGGCCTCGCT ATGACCAGCC ACCCAGGACC 
TCTACCCTGC GGCTGGAAGA GCCATCCACT CCTGCCTATG ATGCACATAG TAGGTACAGG 
CCTGAGGCTC AGCCCTATCC ATCTGCCAGC CTGAAGGTCG CAGAGCCTAA GCAGTATTTT 
GACCAGTATT CACGAAGTTA TGAGCAGGTC CCGTCCCAAG GACTCGGCTC TAAAGCAGGC 
CATTACGAGC CCCTCCACGG TGCTGCTGTC CCTCCTCTCA CCCCTGCACC TCAGCACAAG 
CCTGAAGTTC TCCCTTCAAG CACCAAACCG CTGCCTCCGC CCCCGGCTCT GGCTGAGGAT 
GAAGAAGACC CAGCCATGAA GCCGCAGTCA GTGCTCACCA GAGTAAAAAT GTTCGAGAAC 
AGAAGATCTG CTTCCGTGGA GAGCAAGAAG GATGACAGTC ATCCTGCTGG TTTGAAGCCT 
CCAGAGGGAG CATCTAAGCC TGCAGGTGCT CCCATCGTGG GTCCCAAAGG TCCTGCACAG 
AGTCAGCTCA GCGAACATGA CAGAACACTG CACAGAATGC CAGAACCTCA AAAACCCCAA 
GTGAAGCCAC CTGAAGATAT TGTCCGGTCA AATCATTATG ATCCTGAGGA GGATGAAGAG 
TATTACCGAA AACAGCTCTC ATACTTTGAT CGAAGAAGTT TTGAGAACAA GCCTTCTGCA 
CACGTGCCTG CCAGCCATCT CTCAGAGCCT GCCAAGTCAG TTCATTCTCA GAATCAGCCA 
CATTTTTCAA GTTATCCTTC CAAGGTAAAG TCTGCTGAGG CTGAAGCTGT AGACAGGTCG 
TTTGGGGAGA AGCGCTATGA CCCACTGCAG GCAACACCCC CTCCTCCGCC ATTGCCCACC 
CAGTACAGCC AGCCCTCTCA GCCCGGCCCC AGCACTGCCC TTGGCCTCCA CGTACATGCC 
AAGGGGGCGC ACGGCGAAGG TAATTCAGTG TCATTGGATT TTCAAAACTC ATTAGTGTCT 
AAACCAGACC CACCTCCTGC TCAGAATAAG CCAGCAACTT ACAGACCACC AAACCGAGAA 
GATACTGCTC AGTCTGCTTT CTATCCCCAG AAAAGTTTCC CAGAAAAAGG TCCAGCCAAT 
GGGGCCGAAC AGACTCAGAA AACGATCACT CCAGCATACA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAGTT CAATCACAAT 
CTTCTGCCAA GTGAAATCAT ACATAAACCT GAATTGTCTT CCAAAGCCCC TGCTTCTCCA 
AAAACTCTTG TGAAAACACA TGGTTCAGTG CCGCCTCCTG AGTTTGATAG TGGCGTTGAC 
ACGTTCTCTG TCCATGCAGA TAAGCCTAAA TATCAAGTAA ATAACATCAG CACAGTGCCT 
AAAGCTGTTC CTGTGAGTCC TTCAGCTGTA GAAGATGATG AAGATGAGGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAACGGCG GGGTGTTGAG CTCCATAGAA 
ACTGGCGTTA GCATAATTAT CCCACAGGGA GCTATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGTGAA 
ACCCTGCTGA GCCCCTTAGT GATGTGCGGG CCCCATGGTC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTC TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098886.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954545.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X13, mRNA.

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CloneID	OSe230364
Clone ID Related Accession (Same CDS sequence)	XM_021098886.1
Accession Version	XM_021098886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTCTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954545.1
CDS	459..5489
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098886.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098886.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTCTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098891.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954550.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X14, mRNA.

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CloneID	OSe230365
Clone ID Related Accession (Same CDS sequence)	XM_021098891.1
Accession Version	XM_021098891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954550.1
CDS	438..5447
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098891.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098891.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CTGCAGAAGG GCAGCTGCAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACACGCTTTT GCTGTACAGC AATTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG CAGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGACCCGG TGTCTGAGAA TGAAGAGGAT AGTTATGAGG AAGAAGCACA CGACCCAAGA 
AGTGGCCGTG GGGGTCCTAA CCATAGGAGG AGTGAGAAGA GCTGGGCCAG GGAGAGAAGT 
GCCAGTAGGG ATAAGAGTCT GTCTCCAAGA TCCGATAGGC GGTCTGTGGC CTCCAGTCAA 
CCCACCAAAC CCACCAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC AGAAAATATG 
TCATTGACGG ATGCAAAAAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGGGCTAC ACTGTTGAAC GTCCCTGATC TTTCTGACAG TATCCACTCT 
GCTAATGCTT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGGCGATCAC ACGACAGGCC TCCCCGTCAC AGCCGGTCAC GGTCTCCTGA TCAGCGGTCA 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGGAGG AGAGAACTAC TAAACCCGGG GCAATCTCAA CTCCTGTAAA GCATGCAGAT 
GATCATACAT CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGAC AACCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTAT CCTACCTAAT TCAACTCATG AAGATGGAAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGAAAAGGT GATAGTGTGG GTTTGCGACT GGCTGGTGGA 
AATGACGTTG GAATATTTGT AGCTGGCGTT CTTGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAGAT TCTCAGGGTA AATAATGTGG ACTTTACAAA TATAATAAGA 
GAAGAAGCTG TCCTTTTCCT ACTTGACCTC CCGAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA CCGTCGCATT GTAGAGTCGG ATGTGGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTATTCC GTGTCGTGGA CACTCTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTCGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTCCA ATACACACTT CCAAAAACGG CAGGAGGAGA CCGCGCTGAC 
TTTTGGAGAT TCCGAGGCCT TCGGAGCTCC AAAAGAAATC TTCGCAAGAG CAGGGAAGAC 
TTGTCAGCTC AGCCAGTCCA AACCAAGTTT CCTGCATACG AGAGAGTTGT CCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATCGCAAAAA GTGAACCTCG AGATGCTGGC 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACAA TAAAGCAAAT AATAGATCAA 
GACAAACATG CTTTATTGGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTATGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCG GATTCTAAGC AGGGTGTGAA AACAATGCGA 
ATGAGGCTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACCATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GAGCATTGAA AGAAGCAATT CAACAACAGC AGAACCAGTT GGTATGGGTT 
TCTGAGGGGA AGGCGGATGG CGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCCCCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCAT GAGAACCAAA CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGT TCATAGCATA GACTCCCCTG GACTTAAAGC TGCCTCTCAA 
CAGGTGTATA GGAAGGACCC CTACCCCGAG GAAATGATGA GAGCCAACCA TGTCTTGAAG 
CAGCCAGCTG TCACTCACCC AGGGCAGAGG CCAGACAGAG AGCCGGGTCT GAGCTATGAG 
CCCCAGCCCC CGTACATGGA GAGACAGGCC AGCAGAGACC TGGAGCAGCC TGCGTACAGA 
TATGAGTCCT CCAGCTATAC TGACCAGCTT TCTCGAAACT ATGAACACCG TCTGCGCTGT 
GAAGACCACA CTCCTGCATA TGAGGAGAGG TGGTCCTACT ATGATGACAG GCAGTCCTAC 
CAGCCTCGGC CTGCCCTGGA CAGCCAGCAC CCTCGAGACC TTGACCCTAG ACAGCACCCT 
GAAGAGCCTT CAGAACGAGG GCATTTCCCA CGTTTCGAGG AGCCGACCCC ACCGTCCTAT 
GAAAGCAGGC CTCGCTATGA CCAGCCACCC AGGACCTCTA CCCTGCGGCT GGAAGAGCCA 
TCCACTCCTG CCTATGATGC ACATAGTAGG TACAGGCCTG AGGCTCAGCC CTATCCATCT 
GCCAGCCTGA AGGTCGCAGA GCCTAAGCAG TATTTTGACC AGTATTCACG AAGTTATGAG 
CAGGTCCCGT CCCAAGGACT CGGCTCTAAA GCAGGCCATT ACGAGCCCCT CCACGGTGCT 
GCTGTCCCTC CTCTCACCCC TGCACCTCAG CACAAGCCTG AAGTTCTCCC TTCAAGCACC 
AAACCGCTGC CTCCGCCCCC GGCTCTGGCT GAGGATGAAG AAGACCCAGC CATGAAGCCG 
CAGTCAGTGC TCACCAGAGT AAAAATGTTC GAGAACAGAA GATCTGCTTC CGTGGAGAGC 
AAGAAGGATG ACAGTCATCC TGCTGGTTTG AAGCCTCCAG AGGGAGCATC TAAGCCTGCA 
GGTGCTCCCA TCGTGGGTCC CAAAGGTCCT GCACAGAGTC AGCTCAGCGA ACATGACAGA 
ACACTGCACA GAATGCCAGA ACCTCAAAAA CCCCAAGTGA AGCCACCTGA AGATATTGTC 
CGGTCAAATC ATTATGATCC TGAGGAGGAT GAAGAGTATT ACCGAAAACA GCTCTCATAC 
TTTGATCGAA GAAGTTTTGA GAACAAGCCT TCTGCACACG TGCCTGCCAG CCATCTCTCA 
GAGCCTGCCA AGTCAGTTCA TTCTCAGAAT CAGCCACATT TTTCAAGTTA TCCTTCCAAG 
GTAAAGTCTG CTGAGGCTGA AGCTGTAGAC AGGTCGTTTG GGGAGAAGCG CTATGACCCA 
CTGCAGGCAA CACCCCCTCC TCCGCCATTG CCCACCCAGT ACAGCCAGCC CTCTCAGCCC 
GGCCCCAGCA CTGCCCTTGG CCTCCACGTA CATGCCAAGG GGGCGCACGG CGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA AAACTCATTA GTGTCTAAAC CAGACCCACC TCCTGCTCAG 
AATAAGCCAG CAACTTACAG ACCACCAAAC CGAGAAGATA CTGCTCAGTC TGCTTTCTAT 
CCCCAGAAAA GTTTCCCAGA AAAAGGTCCA GCCAATGGGG CCGAACAGAC TCAGAAAACG 
ATCACTCCAG CATACAATCG ATTCACACCA AAACCATACA CAAGTTCTGC TCGGCCATTT 
GAACGCAAGT TTGAAAGCCC TAAGTTCAAT CACAATCTTC TGCCAAGTGA AATCATACAT 
AAACCTGAAT TGTCTTCCAA AGCCCCTGCT TCTCCAAAAA CTCTTGTGAA AACACATGGT 
TCAGTGCCGC CTCCTGAGTT TGATAGTGGC GTTGACACGT TCTCTGTCCA TGCAGATAAG 
CCTAAATATC AAGTAAATAA CATCAGCACA GTGCCTAAAG CTGTTCCTGT GAGTCCTTCA 
GCTGTAGAAG ATGATGAAGA TGAGGATGGT CACACCGTGG TGGCCACAGC CCGAGGCGTG 
TTTAACAGCA ACGGCGGGGT GTTGAGCTCC ATAGAAACTG GCGTTAGCAT AATTATCCCA 
CAGGGAGCTA TTCCTGAGGG AATTGAGCAG GAAATCTATT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CACCTTTAGA TAAGGAGAAA GGTGAAACCC TGCTGAGCCC CTTAGTGATG 
TGCGGGCCCC ATGGTCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTACC ACACTGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTATCTTGT TGGAGCAAAC 
TGTGTCTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_021098896.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020954555.1 Sus scrofa tight junction protein 1 (TJP1), transcript variant X15, mRNA.

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CloneID	OSe230366
Clone ID Related Accession (Same CDS sequence)	XM_021098896.1
Accession Version	XM_021098896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	tight junction protein ZO-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CAGCTATATG GGAACAACAC ACGGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATAGTGA TTTCAGATGT GCTGAAAGGA GGACCTGCAG AAGGGCAGCT GCAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAATG TTGAACACGC TTTTGCTGTA 
CAGCAATTAA GGAAAAGTGG GAAAAATGCA AAAATTACCA TTCGCAGGAA GAAGAAAGTT 
CAGATACCAG TAAGTCGTCC TGATCCTGAC CCGGTGTCTG AGAATGAAGA GGATAGTTAT 
GAGGAAGAAG CACACGACCC AAGAAGTGGC CGTGGGGGTC CTAACCATAG GAGGAGTGAG 
AAGAGCTGGG CCAGGGAGAG AAGTGCCAGT AGGGATAAGA GTCTGTCTCC AAGATCCGAT 
AGGCGGTCTG TGGCCTCCAG TCAACCCACC AAACCCACCA AAGTCACACT GGTGAAATCC 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA 
AATGGTACCG TGACAGAAAA TATGTCATTG ACGGATGCAA AAACATTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACACTGTT GAACGTCCCT 
GATCTTTCTG ACAGTATCCA CTCTGCTAAT GCTTCTGAGA GAGATGACAT TTCAGAAATT 
CAGTCACTGG CATCAGATCA TTCTGGGCGA TCACACGACA GGCCTCCCCG TCACAGCCGG 
TCACGGTCTC CTGATCAGCG GTCAGAGCCT TCTGACCATT CTAGACACTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAGGAGAGAA CTACTAAACC CGGGGCAATC 
TCAACTCCTG TAAAGCATGC AGATGATCAT ACATCTAAAA CAGTGGAAGA AGTTATAGTT 
GAAAGAAATG AGAAACAAGC ACCCACTCTT CCAGAACCAA AGCCTGTGTA TGCCCAAGTT 
GGACAACCAG ACGTGGATTT ACCTGTCAGT CCATCTGATG GTATCCTACC TAATTCAACT 
CATGAAGATG GAATTCTTCG GCCCAGCATG AAGTTAGTAA AATTCAGAAA AGGTGATAGT 
GTGGGTTTGC GACTGGCTGG TGGAAATGAC GTTGGAATAT TTGTAGCTGG CGTTCTTGAA 
GACAGCCCTG CTGCCAAAGA AGGCTTAGAA GAGGGTGATC AGATTCTCAG GGTAAATAAT 
GTGGACTTTA CAAATATAAT AAGAGAAGAA GCTGTCCTTT TCCTACTTGA CCTCCCGAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTACCGTCG CATTGTAGAG 
TCGGATGTGG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTA TTCCGTGTCG TGGACACTCT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT CGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TCCAATACAC ACTTCCAAAA 
ACGGCAGGAG GAGACCGCGC TGACTTTTGG AGATTCCGAG GCCTTCGGAG CTCCAAAAGA 
AATCTTCGCA AGAGCAGGGA AGACTTGTCA GCTCAGCCAG TCCAAACCAA GTTTCCTGCA 
TACGAGAGAG TTGTCCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATCGCA 
AAAAGTGAAC CTCGAGATGC TGGCACTGAC CAACGTAGCT CTGGCATTAT TCGCCTTCAT 
ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TGGATGTAAC ACCAAATGCA 
GTTGATCGTC TGAATTATGC CCAGTGGTAT CCAATTGTAG TATTCCTTAA CCCGGATTCT 
AAGCAGGGTG TGAAAACAAT GCGAATGAGG CTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAATTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACC 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGAGCAT TGAAAGAAGC AATTCAACAA 
CAGCAGAACC AGTTGGTATG GGTTTCTGAG GGGAAGGCGG ATGGCGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTCAGA GAGGACTCCT CTGGAATGCA TCATGAGAAC 
CAAACATATC CTCCCTACTC ACCACAAGCG CAGCCACAGC CAGTTCATAG CATAGACTCC 
CCTGGACTTA AAGCTGCCTC TCAACAGGTG TATAGGAAGG ACCCCTACCC CGAGGAAATG 
ATGAGAGCCA ACCATGTCTT GAAGCAGCCA GCTGTCACTC ACCCAGGGCA GAGGCCAGAC 
AGAGAGCCGG GTCTGAGCTA TGAGCCCCAG CCCCCGTACA TGGAGAGACA GGCCAGCAGA 
GACCTGGAGC AGCCTGCGTA CAGATATGAG TCCTCCAGCT ATACTGACCA GCTTTCTCGA 
AACTATGAAC ACCGTCTGCG CTGTGAAGAC CACACTCCTG CATATGAGGA GAGGTGGTCC 
TACTATGATG ACAGGCAGTC CTACCAGCCT CGGCCTGCCC TGGACAGCCA GCACCCTCGA 
GACCTTGACC CTAGACAGCA CCCTGAAGAG CCTTCAGAAC GAGGGCATTT CCCACGTTTC 
GAGGAGCCGA CCCCACCGTC CTATGAAAGC AGGCCTCGCT ATGACCAGCC ACCCAGGACC 
TCTACCCTGC GGCTGGAAGA GCCATCCACT CCTGCCTATG ATGCACATAG TAGGTACAGG 
CCTGAGGCTC AGCCCTATCC ATCTGCCAGC CTGAAGGTCG CAGAGCCTAA GCAGTATTTT 
GACCAGTATT CACGAAGTTA TGAGCAGGTC CCGTCCCAAG GACTCGGCTC TAAAGCAGGC 
CATTACGAGC CCCTCCACGG TGCTGCTGTC CCTCCTCTCA CCCCTGCACC TCAGCACAAG 
CCTGAAGTTC TCCCTTCAAG CACCAAACCG CTGCCTCCGC CCCCGGCTCT GGCTGAGGAT 
GAAGAAGACC CAGCCATGAA GCCGCAGTCA GTGCTCACCA GAGTAAAAAT GTTCGAGAAC 
AGAAGATCTG CTTCCGTGGA GAGCAAGAAG GATGACAGTC ATCCTGCTGG TTTGAAGCCT 
CCAGAGGGAG CATCTAAGCC TGCAGGTGCT CCCATCGTGG GTCCCAAAGG TCCTGCACAG 
AGTCAGCTCA GCGAACATGA CAGAACACTG CACAGAATGC CAGAACCTCA AAAACCCCAA 
GTGAAGCCAC CTGAAGATAT TGTCCGGTCA AATCATTATG ATCCTGAGGA GGATGAAGAG 
TATTACCGAA AACAGCTCTC ATACTTTGAT CGAAGAAGTT TTGAGAACAA GCCTTCTGCA 
CACGTGCCTG CCAGCCATCT CTCAGAGCCT GCCAAGTCAG TTCATTCTCA GAATCAGCCA 
CATTTTTCAA GTTATCCTTC CAAGGTAAAG TCTGCTGAGG CTGAAGCTGT AGACAGGTCG 
TTTGGGGAGA AGCGCTATGA CCCACTGCAG GCAACACCCC CTCCTCCGCC ATTGCCCACC 
CAGTACAGCC AGCCCTCTCA GCCCGGCCCC AGCACTGCCC TTGGCCTCCA CGTACATGCC 
AAGGGGGCGC ACGGCGAAGG TAATTCAGTG TCATTGGATT TTCAAAACTC ATTAGTGTCT 
AAACCAGACC CACCTCCTGC TCAGAATAAG CCAGCAACTT ACAGACCACC AAACCGAGAA 
GATACTGCTC AGTCTGCTTT CTATCCCCAG AAAAGTTTCC CAGAAAAAGG TCCAGCCAAT 
GGGGCCGAAC AGACTCAGAA AACGATCACT CCAGCATACA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAGTT CAATCACAAT 
CTTCTGCCAA GTGAAATCAT ACATAAACCT GAATTGTCTT CCAAAGCCCC TGCTTCTCCA 
AAAACTCTTG TGAAAACACA TGGTTCAGTG CCGCCTCCTG AGTTTGATAG TGGCGTTGAC 
ACGTTCTCTG TCCATGCAGA TAAGCCTAAA TATCAAGTAA ATAACATCAG CACAGTGCCT 
AAAGCTGTTC CTGTGAGTCC TTCAGCTGTA GAAGATGATG AAGATGAGGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAACGGCG GGGTGTTGAG CTCCATAGAA 
ACTGGCGTTA GCATAATTAT CCCACAGGGA GCTATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGTGAA 
ACCCTGCTGA GCCCCTTAGT GATGTGCGGG CCCCATGGTC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTC TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020954555.1
CDS	353..5326
Translation	MEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPDPVSENEEDSYEEEAHDPRSGRGGPNHRRSEKSWARERSASRDKSLSPRSDRRSVASSQPTKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERTTKPGAISTPVKHADDHTSKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGILPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVHSIDSPGLKAASQQVYRKDPYPEEMMRANHVLKQPAVTHPGQRPDREPGLSYEPQPPYMERQASRDLEQPAYRYESSSYTDQLSRNYEHRLRCEDHTPAYEERWSYYDDRQSYQPRPALDSQHPRDLDPRQHPEEPSERGHFPRFEEPTPPSYESRPRYDQPPRTSTLRLEEPSTPAYDAHSRYRPEAQPYPSASLKVAEPKQYFDQYSRSYEQVPSQGLGSKAGHYEPLHGAAVPPLTPAPQHKPEVLPSSTKPLPPPPALAEDEEDPAMKPQSVLTRVKMFENRRSASVESKKDDSHPAGLKPPEGASKPAGAPIVGPKGPAQSQLSEHDRTLHRMPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSAHVPASHLSEPAKSVHSQNQPHFSSYPSKVKSAEAEAVDRSFGEKRYDPLQATPPPPPLPTQYSQPSQPGPSTALGLHVHAKGAHGEGNSVSLDFQNSLVSKPDPPPAQNKPATYRPPNREDTAQSAFYPQKSFPEKGPANGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSEIIHKPELSSKAPASPKTLVKTHGSVPPPEFDSGVDTFSVHADKPKYQVNNISTVPKAVPVSPSAVEDDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021098896.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa tight junction protein 1 (TJP1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021098896.1

ORF Insert Sequence:

ATGGAGGAAA CAGCTATATG GGAACAACAC ACGGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGAA TTGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATAGTGA TTTCAGATGT GCTGAAAGGA GGACCTGCAG AAGGGCAGCT GCAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAATG TTGAACACGC TTTTGCTGTA 
CAGCAATTAA GGAAAAGTGG GAAAAATGCA AAAATTACCA TTCGCAGGAA GAAGAAAGTT 
CAGATACCAG TAAGTCGTCC TGATCCTGAC CCGGTGTCTG AGAATGAAGA GGATAGTTAT 
GAGGAAGAAG CACACGACCC AAGAAGTGGC CGTGGGGGTC CTAACCATAG GAGGAGTGAG 
AAGAGCTGGG CCAGGGAGAG AAGTGCCAGT AGGGATAAGA GTCTGTCTCC AAGATCCGAT 
AGGCGGTCTG TGGCCTCCAG TCAACCCACC AAACCCACCA AAGTCACACT GGTGAAATCC 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA 
AATGGTACCG TGACAGAAAA TATGTCATTG ACGGATGCAA AAACATTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACACTGTT GAACGTCCCT 
GATCTTTCTG ACAGTATCCA CTCTGCTAAT GCTTCTGAGA GAGATGACAT TTCAGAAATT 
CAGTCACTGG CATCAGATCA TTCTGGGCGA TCACACGACA GGCCTCCCCG TCACAGCCGG 
TCACGGTCTC CTGATCAGCG GTCAGAGCCT TCTGACCATT CTAGACACTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG GAGGAGAGAA CTACTAAACC CGGGGCAATC 
TCAACTCCTG TAAAGCATGC AGATGATCAT ACATCTAAAA CAGTGGAAGA AGTTATAGTT 
GAAAGAAATG AGAAACAAGC ACCCACTCTT CCAGAACCAA AGCCTGTGTA TGCCCAAGTT 
GGACAACCAG ACGTGGATTT ACCTGTCAGT CCATCTGATG GTATCCTACC TAATTCAACT 
CATGAAGATG GAATTCTTCG GCCCAGCATG AAGTTAGTAA AATTCAGAAA AGGTGATAGT 
GTGGGTTTGC GACTGGCTGG TGGAAATGAC GTTGGAATAT TTGTAGCTGG CGTTCTTGAA 
GACAGCCCTG CTGCCAAAGA AGGCTTAGAA GAGGGTGATC AGATTCTCAG GGTAAATAAT 
GTGGACTTTA CAAATATAAT AAGAGAAGAA GCTGTCCTTT TCCTACTTGA CCTCCCGAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTACCGTCG CATTGTAGAG 
TCGGATGTGG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTA TTCCGTGTCG TGGACACTCT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT CGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TCCAATACAC ACTTCCAAAA 
ACGGCAGGAG GAGACCGCGC TGACTTTTGG AGATTCCGAG GCCTTCGGAG CTCCAAAAGA 
AATCTTCGCA AGAGCAGGGA AGACTTGTCA GCTCAGCCAG TCCAAACCAA GTTTCCTGCA 
TACGAGAGAG TTGTCCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATCGCA 
AAAAGTGAAC CTCGAGATGC TGGCACTGAC CAACGTAGCT CTGGCATTAT TCGCCTTCAT 
ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TGGATGTAAC ACCAAATGCA 
GTTGATCGTC TGAATTATGC CCAGTGGTAT CCAATTGTAG TATTCCTTAA CCCGGATTCT 
AAGCAGGGTG TGAAAACAAT GCGAATGAGG CTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAATTATATG AACGGTCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACC 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGAGCAT TGAAAGAAGC AATTCAACAA 
CAGCAGAACC AGTTGGTATG GGTTTCTGAG GGGAAGGCGG ATGGCGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC CCCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTCAGA GAGGACTCCT CTGGAATGCA TCATGAGAAC 
CAAACATATC CTCCCTACTC ACCACAAGCG CAGCCACAGC CAGTTCATAG CATAGACTCC 
CCTGGACTTA AAGCTGCCTC TCAACAGGTG TATAGGAAGG ACCCCTACCC CGAGGAAATG 
ATGAGAGCCA ACCATGTCTT GAAGCAGCCA GCTGTCACTC ACCCAGGGCA GAGGCCAGAC 
AGAGAGCCGG GTCTGAGCTA TGAGCCCCAG CCCCCGTACA TGGAGAGACA GGCCAGCAGA 
GACCTGGAGC AGCCTGCGTA CAGATATGAG TCCTCCAGCT ATACTGACCA GCTTTCTCGA 
AACTATGAAC ACCGTCTGCG CTGTGAAGAC CACACTCCTG CATATGAGGA GAGGTGGTCC 
TACTATGATG ACAGGCAGTC CTACCAGCCT CGGCCTGCCC TGGACAGCCA GCACCCTCGA 
GACCTTGACC CTAGACAGCA CCCTGAAGAG CCTTCAGAAC GAGGGCATTT CCCACGTTTC 
GAGGAGCCGA CCCCACCGTC CTATGAAAGC AGGCCTCGCT ATGACCAGCC ACCCAGGACC 
TCTACCCTGC GGCTGGAAGA GCCATCCACT CCTGCCTATG ATGCACATAG TAGGTACAGG 
CCTGAGGCTC AGCCCTATCC ATCTGCCAGC CTGAAGGTCG CAGAGCCTAA GCAGTATTTT 
GACCAGTATT CACGAAGTTA TGAGCAGGTC CCGTCCCAAG GACTCGGCTC TAAAGCAGGC 
CATTACGAGC CCCTCCACGG TGCTGCTGTC CCTCCTCTCA CCCCTGCACC TCAGCACAAG 
CCTGAAGTTC TCCCTTCAAG CACCAAACCG CTGCCTCCGC CCCCGGCTCT GGCTGAGGAT 
GAAGAAGACC CAGCCATGAA GCCGCAGTCA GTGCTCACCA GAGTAAAAAT GTTCGAGAAC 
AGAAGATCTG CTTCCGTGGA GAGCAAGAAG GATGACAGTC ATCCTGCTGG TTTGAAGCCT 
CCAGAGGGAG CATCTAAGCC TGCAGGTGCT CCCATCGTGG GTCCCAAAGG TCCTGCACAG 
AGTCAGCTCA GCGAACATGA CAGAACACTG CACAGAATGC CAGAACCTCA AAAACCCCAA 
GTGAAGCCAC CTGAAGATAT TGTCCGGTCA AATCATTATG ATCCTGAGGA GGATGAAGAG 
TATTACCGAA AACAGCTCTC ATACTTTGAT CGAAGAAGTT TTGAGAACAA GCCTTCTGCA 
CACGTGCCTG CCAGCCATCT CTCAGAGCCT GCCAAGTCAG TTCATTCTCA GAATCAGCCA 
CATTTTTCAA GTTATCCTTC CAAGGTAAAG TCTGCTGAGG CTGAAGCTGT AGACAGGTCG 
TTTGGGGAGA AGCGCTATGA CCCACTGCAG GCAACACCCC CTCCTCCGCC ATTGCCCACC 
CAGTACAGCC AGCCCTCTCA GCCCGGCCCC AGCACTGCCC TTGGCCTCCA CGTACATGCC 
AAGGGGGCGC ACGGCGAAGG TAATTCAGTG TCATTGGATT TTCAAAACTC ATTAGTGTCT 
AAACCAGACC CACCTCCTGC TCAGAATAAG CCAGCAACTT ACAGACCACC AAACCGAGAA 
GATACTGCTC AGTCTGCTTT CTATCCCCAG AAAAGTTTCC CAGAAAAAGG TCCAGCCAAT 
GGGGCCGAAC AGACTCAGAA AACGATCACT CCAGCATACA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAACGC AAGTTTGAAA GCCCTAAGTT CAATCACAAT 
CTTCTGCCAA GTGAAATCAT ACATAAACCT GAATTGTCTT CCAAAGCCCC TGCTTCTCCA 
AAAACTCTTG TGAAAACACA TGGTTCAGTG CCGCCTCCTG AGTTTGATAG TGGCGTTGAC 
ACGTTCTCTG TCCATGCAGA TAAGCCTAAA TATCAAGTAA ATAACATCAG CACAGTGCCT 
AAAGCTGTTC CTGTGAGTCC TTCAGCTGTA GAAGATGATG AAGATGAGGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAACGGCG GGGTGTTGAG CTCCATAGAA 
ACTGGCGTTA GCATAATTAT CCCACAGGGA GCTATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGTGAA 
ACCCTGCTGA GCCCCTTAGT GATGTGCGGG CCCCATGGTC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTC TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TJP1
Entrez Gene ID	100736682
Full Name	tight junction protein 1
Synonyms	zo1
Gene Type	protein-coding
Organism	Sus scrofa(pig)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TJP1 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

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