ORF » Species Summary » Strongylocentrotus purpuratus » LOC105442324 cDNA ORF clone

LOC105442324 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

The following LOC105442324 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105442324 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSd37451 XM_011674313.1
Latest version!		 Strongylocentrotus purpuratus E3 ubiquitin-protein ligase MIB1-like (LOC105442324), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OSd52790 XM_030986524.1
Latest version!		 Strongylocentrotus purpuratus E3 ubiquitin-protein ligase MIB2-like (LOC105442324), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC105442324 ( XM_011674313.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011672615.1 Strongylocentrotus purpuratus E3 ubiquitin-protein ligase MIB1-like (LOC105442324), mRNA.
    Price & Availability↑
    CloneID OSd37451
    Clone ID Related Accession (Same CDS sequence) XM_011674313.1
    Accession Version XM_011674313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1427040000000
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Product E3 ubiquitin-protein ligase MIB1-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011986068.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATCTTC AAGAAAACAG TCAAAATGGA TGGGTCGATG GCATGGAAAA GTGTCTAGGA 
    ATGGAAGGGA CGGTGATGAA CGTTGACTCA CGTTACAACA GTCGGGTACG ATTGATTGGT 
    TTGGAGAATA TTTGGTCATG GCATCCCTGC TGCTTGGTCC TCGTCAAGAA GAACTCATCA 
    GATGATGACG AGCAAGGTGC TGAATCTCTA GGCCTCAAGC TAGATGGAAG TGGACATACT 
    GAAATGCTCA AGGCCTTGCT ATCACGACTC AAGACAGAAG CTAACAGCTC ATCCGATAGT 
    GATTCAGATG AAGGAGGTGA CAGAGATGGA AGTGGACAAA CTAAAATGCT CAAAGCCTTG 
    CTATCACTAC TCAAGACAAA GGCTGGCAGC TCATCCGATA GTGATTCAGG CGAAGGAGAT 
    GAAGAATCTC AACTGATCAA GGCTGCTAAG AGGGGCAAAG TTGAACGTGT TGTGGAGATT 
    CTTTCCACAT CACCAGAAAA GGCAAATGTG AAAGTTGGTG GGAAGACTGC TCTACACAAA 
    GCAGCTGCCC AGGGTCATCT TGAAGTAGTC CAGGCACTTC TGGAGTCAGG GGCTGAGATA 
    GATATCACCA CTGATGATGG CGGGAACACT CCACTTATTT ACTCTGTCGA AGGGGATGAA 
    CCAGCTATCA CAAAGTATTT GATTGGTAAA GGAGCGGATA TAAACAGAGG TAATGACAAC 
    GGACGGAGAG CGATCCATCA AGCAGCTTAC AAAGGGCTTG TTGACTGTGC AAGGGTCTTG 
    ATTAATCATG GATGTGACGT TAATGTACAG GATACAGAGA AAGATACTCC CCTCCATGAT 
    GCCATAATGA AGAGCAGGTT AGATGTCATT GAACTATTGG TGAAAGTTCC AGACCTCGAT 
    GTTACTATTC CTGACGAGAG AGGGTTTACA GCTCTACAGT TTGCAGCTTT AAAAGACAAA 
    CCAGAAGCTG CTGAGTTAAT CGCGAGAAGT TGCCCTCAAT CTATTGTTGT TGCAAAGGAT 
    GATGGCTTCA ACATACTGCA TATCTGTGCA GTCAATAACC ATGTAGAAGT CATGAAGGCT 
    GTTATGGCTA TGAAGGATCA CGGGTTGGAT GTGAATGCAA AGGAATCGCG AGGACTGACA 
    GCACTACACC TGGCTGCTAA CGATGGATAT TCCCATTGCA TAGAGTTTTT GATGTCACAG 
    GGTGCTGATA TCAATCTTCG AGGTAAAGAT GATCACACTG CCCTGCACAT CCTTGCCAGC 
    ACTGCAGGGA AAGATCCAAG TGATATCAAA GACACTCCTA CACTGAGGAA GATTCGCAAG 
    CGTTTTAGAG ATTGTGGAGT TGATAACAGT GCTGCCGCTG CACTCTGCTA CATGGTTCTT 
    AAAGGAGCGT CCATAGATCT AGAGAATGTC AAATGTAGAA CACCGCTATA TTACATCAGG 
    AATGCTGAGC TGAAGAAGAC TTTGAAGAAG ATTGCTCGTG AAAAAACATC TGACGATATG 
    TCATCATGGG AGGACCTCGG GGAAGATGAG GATGTGAAGA AGGGACGCCT GAGCAGTAAC 
    TCCGTCAATG TTGATGACGA TGATATTATC GTAAAGCGTA ACGATGGTCT GAGCGGGACA 
    GACAAAGGCT CACCAAATCG AATAAGACAG GACTCGGCTG AGAGTGAAAG TGGAGCTGAT 
    GGTCCAGCAG TAAACCAAAA GAAAACTGAC AAGCCAAAAA TTGAAGTCTT CGATCTTGTG 
    CACAAAATTG AAGTCTCTGA CCTAGACATG GGTCCTGTTG TTGGGAAGGG GCAGTTTGGA 
    GTGGTCCATC GAGCCTCCTG GAGAGGGACT CCAGTGGCAG TCAAAAAGAT CTCCATGATG 
    GGTAGCAGAA AAGATGAAAT CGAGAAGGAA GTCGCAATAC ACAGACGGGC ATGTCATCCT 
    AACATCGTTC AGATGATGGC TCTAGGATAC CAGGGAAATG AGGCCTTCCT GGTAATGCAG 
    TTCATTGAAG GACCAAGTTT GCACACGGTC ATTTTCCCTG ATAGGGATGA CGTTAAGATT 
    CCTCTAGACT GGGCTAAACA GCTTCAAATA TCTTGTGAGG TTCTCCAAGC CGTAACCTTC 
    ATGCATGCCT GCTACATCTT ACATCTTGAT ATTAAGCCTG CCAATATTCT AGTGGACTCT 
    GCAACAATTC GTCCCTACAT CTGTGACTTG GGCCTGGCCC ACATCAAGAA CCGCAACCTC 
    ATGTCCCAAT CCGCAGTCAA CATGAGAGGC ACACCGTGCT TCATGCCCCC AGAGGCACTT 
    GGCGCAACGG AACCTGGCTA CAGGCACTCC AACAAACATG ACATCTGGTC GGTTGCAGGA 
    ACATTAGTGG AGTTGTACTC CATGAAGCGC CTGTGGGGAG ACTCGATGAA TCCTCTGGTC 
    CTGATGGCTC GTATTTATAC AGGCCAGATG GGTAAACCAG ATTCTTTGAC AGCTGTAGAC 
    CCTAAGGTTC AGAAGATCCT TGAACCATGC TTCGAACAGA AGCCAGAGAA GAGACCATCA 
    GCTTCTAATC TCCTGGATCA GTTTAATGAC CTAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011672615.1
    CDS52..2610
    Translation

    Target ORF information:

    RefSeq Version XM_011674313.1
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Definition Strongylocentrotus purpuratus E3 ubiquitin-protein ligase MIB1-like (LOC105442324), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011674313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATCTTC AAGAAAACAG TCAAAATGGA TGGGTCGATG GCATGGAAAA GTGTCTAGGA 
    ATGGAAGGGA CGGTGATGAA CGTTGACTCA CGTTACAACA GTCGGGTACG ATTGATTGGT 
    TTGGAGAATA TTTGGTCATG GCATCCCTGC TGCTTGGTCC TCGTCAAGAA GAACTCATCA 
    GATGATGACG AGCAAGGTGC TGAATCTCTA GGCCTCAAGC TAGATGGAAG TGGACATACT 
    GAAATGCTCA AGGCCTTGCT ATCACGACTC AAGACAGAAG CTAACAGCTC ATCCGATAGT 
    GATTCAGATG AAGGAGGTGA CAGAGATGGA AGTGGACAAA CTAAAATGCT CAAAGCCTTG 
    CTATCACTAC TCAAGACAAA GGCTGGCAGC TCATCCGATA GTGATTCAGG CGAAGGAGAT 
    GAAGAATCTC AACTGATCAA GGCTGCTAAG AGGGGCAAAG TTGAACGTGT TGTGGAGATT 
    CTTTCCACAT CACCAGAAAA GGCAAATGTG AAAGTTGGTG GGAAGACTGC TCTACACAAA 
    GCAGCTGCCC AGGGTCATCT TGAAGTAGTC CAGGCACTTC TGGAGTCAGG GGCTGAGATA 
    GATATCACCA CTGATGATGG CGGGAACACT CCACTTATTT ACTCTGTCGA AGGGGATGAA 
    CCAGCTATCA CAAAGTATTT GATTGGTAAA GGAGCGGATA TAAACAGAGG TAATGACAAC 
    GGACGGAGAG CGATCCATCA AGCAGCTTAC AAAGGGCTTG TTGACTGTGC AAGGGTCTTG 
    ATTAATCATG GATGTGACGT TAATGTACAG GATACAGAGA AAGATACTCC CCTCCATGAT 
    GCCATAATGA AGAGCAGGTT AGATGTCATT GAACTATTGG TGAAAGTTCC AGACCTCGAT 
    GTTACTATTC CTGACGAGAG AGGGTTTACA GCTCTACAGT TTGCAGCTTT AAAAGACAAA 
    CCAGAAGCTG CTGAGTTAAT CGCGAGAAGT TGCCCTCAAT CTATTGTTGT TGCAAAGGAT 
    GATGGCTTCA ACATACTGCA TATCTGTGCA GTCAATAACC ATGTAGAAGT CATGAAGGCT 
    GTTATGGCTA TGAAGGATCA CGGGTTGGAT GTGAATGCAA AGGAATCGCG AGGACTGACA 
    GCACTACACC TGGCTGCTAA CGATGGATAT TCCCATTGCA TAGAGTTTTT GATGTCACAG 
    GGTGCTGATA TCAATCTTCG AGGTAAAGAT GATCACACTG CCCTGCACAT CCTTGCCAGC 
    ACTGCAGGGA AAGATCCAAG TGATATCAAA GACACTCCTA CACTGAGGAA GATTCGCAAG 
    CGTTTTAGAG ATTGTGGAGT TGATAACAGT GCTGCCGCTG CACTCTGCTA CATGGTTCTT 
    AAAGGAGCGT CCATAGATCT AGAGAATGTC AAATGTAGAA CACCGCTATA TTACATCAGG 
    AATGCTGAGC TGAAGAAGAC TTTGAAGAAG ATTGCTCGTG AAAAAACATC TGACGATATG 
    TCATCATGGG AGGACCTCGG GGAAGATGAG GATGTGAAGA AGGGACGCCT GAGCAGTAAC 
    TCCGTCAATG TTGATGACGA TGATATTATC GTAAAGCGTA ACGATGGTCT GAGCGGGACA 
    GACAAAGGCT CACCAAATCG AATAAGACAG GACTCGGCTG AGAGTGAAAG TGGAGCTGAT 
    GGTCCAGCAG TAAACCAAAA GAAAACTGAC AAGCCAAAAA TTGAAGTCTT CGATCTTGTG 
    CACAAAATTG AAGTCTCTGA CCTAGACATG GGTCCTGTTG TTGGGAAGGG GCAGTTTGGA 
    GTGGTCCATC GAGCCTCCTG GAGAGGGACT CCAGTGGCAG TCAAAAAGAT CTCCATGATG 
    GGTAGCAGAA AAGATGAAAT CGAGAAGGAA GTCGCAATAC ACAGACGGGC ATGTCATCCT 
    AACATCGTTC AGATGATGGC TCTAGGATAC CAGGGAAATG AGGCCTTCCT GGTAATGCAG 
    TTCATTGAAG GACCAAGTTT GCACACGGTC ATTTTCCCTG ATAGGGATGA CGTTAAGATT 
    CCTCTAGACT GGGCTAAACA GCTTCAAATA TCTTGTGAGG TTCTCCAAGC CGTAACCTTC 
    ATGCATGCCT GCTACATCTT ACATCTTGAT ATTAAGCCTG CCAATATTCT AGTGGACTCT 
    GCAACAATTC GTCCCTACAT CTGTGACTTG GGCCTGGCCC ACATCAAGAA CCGCAACCTC 
    ATGTCCCAAT CCGCAGTCAA CATGAGAGGC ACACCGTGCT TCATGCCCCC AGAGGCACTT 
    GGCGCAACGG AACCTGGCTA CAGGCACTCC AACAAACATG ACATCTGGTC GGTTGCAGGA 
    ACATTAGTGG AGTTGTACTC CATGAAGCGC CTGTGGGGAG ACTCGATGAA TCCTCTGGTC 
    CTGATGGCTC GTATTTATAC AGGCCAGATG GGTAAACCAG ATTCTTTGAC AGCTGTAGAC 
    CCTAAGGTTC AGAAGATCCT TGAACCATGC TTCGAACAGA AGCCAGAGAA GAGACCATCA 
    GCTTCTAATC TCCTGGATCA GTTTAATGAC CTAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC105442324 ( XM_030986524.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030842384.1 Strongylocentrotus purpuratus E3 ubiquitin-protein ligase MIB2-like (LOC105442324), mRNA.
    Price & Availability↑
    CloneID OSd52790
    Clone ID Related Accession (Same CDS sequence) XM_030986524.1
    Accession Version XM_030986524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1569427200000
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Product E3 ubiquitin-protein ligase MIB2-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145601.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAACCG TGGAGGTCGG ACAGCGAGTT GTCCGGAGCG CCACCTGGAC ATCGGGAGAT 
    CAAGATGGCG GTGAGGGTCA TCTTGGGACA GTGGTCAAGC TAAAAGATCA AGACCCTCAG 
    GATCCTATTC CTATAGGCTG GGCCAAGATC AGATGGGATA ATGGTAATGT TAACAACTAT 
    CAGGTCGGGT CCAGTGGTTC ATATGATCTT GCGCTCTTCG ACAGTGCCCC CGCAGGTGTG 
    ATTCATGTTG GTATAGTTTG TGATAGCTGT TCAGTGAATC CTATAGCTGG CATACGCTGG 
    AAATGCAGTG ATCAAAGCTG CCCTGATTAT GACCTTTGTA CTCCATGCTA CATGAATGAT 
    AAACACGACT TGACTCATAC ATTCACTCGC TTCACCAGCC GGAAAGAAAC AGGGACACCA 
    GTAACACCTC GATTTAGAGA AGCGAAGACA GTTTCCAGAG GGCTGTGTCC AGGAGCAGAG 
    GTCTGTAGAG GGAAGGATTG GCAAGGAGAT GATAAAGATG GCGACAGTGG GACTATGGTT 
    GAGATATGTC CCTTCTCTGA AAGGCCAAGG TCAGGTGTCA CTGTTTGCTG GACTCAGTCT 
    AAACTGGTCA CTACTCATAG AGTTGGATTT GATGGAAAAA TGGATCTGAA ATGCACCACA 
    GCAGGAACAG GGATAGGTTA CTACGCTACA CATCTCCCAG TTTTAGGTAA AGAGGTTGGT 
    TTGAAGGTTG GCGATCTGGT AGCTATTACT CTGGACAGGG AAACATTGCA GATTATGCAA 
    CAGATGGATG GGCGAGAGTG GTTACCAGAA ATGGGAAATC TGTATGGGAA GGTTGCCAAG 
    GTTACTGGTA TCAACTTAAA CGGGAACGCA GAGGTCAATT ACCCTGGGAA AAAAGAACAA 
    CCTTTCACCT TCAATCCAGA TGTACTGAAC AAGGTACCGA GGCTAGGTAA GGGGGACCGT 
    GTGCAGATCA ATAAAGACTT GACGTTTGTG ATGAATCTTC AAGAAAACAG TCAAAATGGA 
    TGGGTCGATG GCATGGAAAA GTGTCTAGGA ATGGAAGGGA CGGTGATGAA CGTTGACTCA 
    CGTTACAACA GTCGGGTACG ATTGATTGGT TTGGAGAATA TTTGGTCATG GCATCCCTGC 
    TGCTTGGTCC TCGTCAAGAA GAACTCATCA GATGATGACG AGCAAGGTGC TGAATCTCTA 
    GGCCTCAAGC TAGATGGAAG TGGACATACT GAAATGCTCA AGGCCTTGCT ATCACGACTC 
    AAGACAGAAG CTAACAGCTC ATCCGATAGT GATTCAGATG AAGGAGGTGA CAGAGATGGA 
    AGTGGACAAA CTAAAATGCT CAAAGCCTTG CTATCACTAC TCAAGACAAA GGCTGGCAGC 
    TCATCCGATA GTGATTCAGG CGAAGGAGAT GAAGAATCTC AACTGATCAA GGCTGCTAAG 
    AGGGGCAAAG TTGAACGTGT TGTGGAGATT CTTTCCACAT CACCAGAAAA GGCAAATGTG 
    AAAGTTGGTG GGAAGACTGC TCTACACAAA GCAGCTGCCC AGGGTCATCT TGAAGTAGTC 
    CAGGCACTTC TGGAGTCAGG GGCTGAGATA GATATCACCA CTGATGATGG CGGGAACACT 
    CCACTTATTT ACTCTGTCGA AGGGGATGAA CCAGCTATCA CAAAGTATTT GATTGGTAAA 
    GGAGCGGATA TAAACAGAGG TAATGACAAC GGACGGAGAG CGATCCATCA AGCAGCTTAC 
    AAAGGGCTTG TTGACTGTGC AAGGGTCTTG ATTAATCATG GATGTGACGT TAATGTACAG 
    GATACAGAGA AAGATACTCC CCTCCATGAT GCCATAATGA AGAGCAGGTT AGATGTCATT 
    GAACTATTGG TGAAAGTTCC AGACCTCGAT GTTACTATTC CTGACGAGAG AGGGTTTACA 
    GCTCTACAGT TTGCAGCTTT AAAAGACAAA CCAGAAGCTG CTGAGTTAAT CGCGAGAAGT 
    TGCCCTCAAT CTATTGTTGT TGCAAAGGAT GATGGCTTCA ACATACTGCA TATCTGTGCA 
    GTCAATAACC ATGTAGAAGT CATGAAGGCT GTTATGGCTA TGAAGGATCA CGGGTTGGAT 
    GTGAATGCAA AGGAATCGCG AGGACTGACA GCACTACACC TGGCTGCTAA CGATGGATAT 
    TCCCATTGCA TAGAGTTTTT GATGTCACAG GGTGCTGATA TCAATCTTCG AGGTAAAGAT 
    GATCACACTG CCCTGCACAT CCTTGCCAGC ACTGCAGGGA AAGATCCAAG TGATATCAAA 
    GACACTCCTA CACTGAGGAA GATTCGCAAG CGTTTTAGAG ATTGTGGAGT TGATAACAGT 
    GCTGCCGCTG CACTCTGCTA CATGGTTCTT AAAGGAGCGT CCATAGATCT AGAGAATGTC 
    AAATGTAGAA CACCGCTATA TTACATCAGG AATGCTGAGC TGAAGAAGAC TTTGAAGAAG 
    ATTGCTCGTG AAAAAACATC TGACGATATG TCATCATGGG AGGACCTCGG GGAAGATGAG 
    GATGTGAAGA AGGGACGCCT GAGCAGTAAC TCCGTCAATG TTGATGACGA TGATATTATC 
    GTAAAGCGTA ACGATGGTCT GAGCGGGACA GACAAAGGCT CACCAAATCG AATAAGACAG 
    GACTCGGCTG AGAGTGAAAG TGGAGCTGAT GGTCCAGCAG TAAACCAAAA GAAAACTGAC 
    AAGCCAAAAA TTGAAGTCTT CGATCTTGTG CACAAAATTG AAGTCTCTGA CCTAGACATG 
    GGTCCTGTTG TTGGGAAGGG GCAGTTTGGA GTGGTCCATC GAGCCTCCTG GAGAGGGACT 
    CCAGTGGCAG TCAAAAAGAT CTCCATGATG GGTAGCAGAA AAGATGAAAT CGAGAAGGAA 
    GTCGCAATAC ACAGACGGGC ATGTCATCCT AACATCGTTC AGATGATGGC TCTAGGATAC 
    CAGGGAAATG AGGCCTTCCT GGTAATGCAG TTCATTGAAG GACCAAGTTT GCACACGGTC 
    ATTTTCCCTG ATAGGGATGA CGTTAAGATT CCTCTAGACT GGGCTAAACA GCTTCAAATA 
    TCTTGTGAGG TTCTCCAAGC CGTAACCTTC ATGCATGCCT GCTACATCTT ACATCTTGAT 
    ATTAAGCCTG CCAATATTCT AGTGGACTCT GCAACAATTC GTCCCTACAT CTGTGACTTG 
    GGCCTGGCCC ACATCAAGAA CCGCAACCTC ATGTCCCAAT CCGCAGTCAA CATGAGAGGC 
    ACACCGTGCT TCATGCCCCC AGAGGCACTT GGCGCAACGG AACCTGGCTA CAGGCACTCC 
    AACAAACATG ACATCTGGTC GGTTGCAGGA ACATTAGTGG AGTTGTACTC CATGAAGCGC 
    CTGTGGGGAG ACTCGATGAA TCCTCTGGTC CTGATGGCTC GTATTTATAC AGGCCAGATG 
    GGTAAACCAG ATTCTTTGAC AGCTGTAGAC CCTAAGGTTC AGAAGATCCT TGAACCATGC 
    TTCGAACAGA AGCCAGAGAA GAGACCATCA GCTTCTAATC TCCTGGATCA GTTTAATGAC 
    CTAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030842384.1
    CDS33..3581
    Translation

    Target ORF information:

    RefSeq Version XM_030986524.1
    Organism Strongylocentrotus purpuratus(purple sea urchin)
    Definition Strongylocentrotus purpuratus E3 ubiquitin-protein ligase MIB2-like (LOC105442324), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_030986524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAACCG TGGAGGTCGG ACAGCGAGTT GTCCGGAGCG CCACCTGGAC ATCGGGAGAT 
    CAAGATGGCG GTGAGGGTCA TCTTGGGACA GTGGTCAAGC TAAAAGATCA AGACCCTCAG 
    GATCCTATTC CTATAGGCTG GGCCAAGATC AGATGGGATA ATGGTAATGT TAACAACTAT 
    CAGGTCGGGT CCAGTGGTTC ATATGATCTT GCGCTCTTCG ACAGTGCCCC CGCAGGTGTG 
    ATTCATGTTG GTATAGTTTG TGATAGCTGT TCAGTGAATC CTATAGCTGG CATACGCTGG 
    AAATGCAGTG ATCAAAGCTG CCCTGATTAT GACCTTTGTA CTCCATGCTA CATGAATGAT 
    AAACACGACT TGACTCATAC ATTCACTCGC TTCACCAGCC GGAAAGAAAC AGGGACACCA 
    GTAACACCTC GATTTAGAGA AGCGAAGACA GTTTCCAGAG GGCTGTGTCC AGGAGCAGAG 
    GTCTGTAGAG GGAAGGATTG GCAAGGAGAT GATAAAGATG GCGACAGTGG GACTATGGTT 
    GAGATATGTC CCTTCTCTGA AAGGCCAAGG TCAGGTGTCA CTGTTTGCTG GACTCAGTCT 
    AAACTGGTCA CTACTCATAG AGTTGGATTT GATGGAAAAA TGGATCTGAA ATGCACCACA 
    GCAGGAACAG GGATAGGTTA CTACGCTACA CATCTCCCAG TTTTAGGTAA AGAGGTTGGT 
    TTGAAGGTTG GCGATCTGGT AGCTATTACT CTGGACAGGG AAACATTGCA GATTATGCAA 
    CAGATGGATG GGCGAGAGTG GTTACCAGAA ATGGGAAATC TGTATGGGAA GGTTGCCAAG 
    GTTACTGGTA TCAACTTAAA CGGGAACGCA GAGGTCAATT ACCCTGGGAA AAAAGAACAA 
    CCTTTCACCT TCAATCCAGA TGTACTGAAC AAGGTACCGA GGCTAGGTAA GGGGGACCGT 
    GTGCAGATCA ATAAAGACTT GACGTTTGTG ATGAATCTTC AAGAAAACAG TCAAAATGGA 
    TGGGTCGATG GCATGGAAAA GTGTCTAGGA ATGGAAGGGA CGGTGATGAA CGTTGACTCA 
    CGTTACAACA GTCGGGTACG ATTGATTGGT TTGGAGAATA TTTGGTCATG GCATCCCTGC 
    TGCTTGGTCC TCGTCAAGAA GAACTCATCA GATGATGACG AGCAAGGTGC TGAATCTCTA 
    GGCCTCAAGC TAGATGGAAG TGGACATACT GAAATGCTCA AGGCCTTGCT ATCACGACTC 
    AAGACAGAAG CTAACAGCTC ATCCGATAGT GATTCAGATG AAGGAGGTGA CAGAGATGGA 
    AGTGGACAAA CTAAAATGCT CAAAGCCTTG CTATCACTAC TCAAGACAAA GGCTGGCAGC 
    TCATCCGATA GTGATTCAGG CGAAGGAGAT GAAGAATCTC AACTGATCAA GGCTGCTAAG 
    AGGGGCAAAG TTGAACGTGT TGTGGAGATT CTTTCCACAT CACCAGAAAA GGCAAATGTG 
    AAAGTTGGTG GGAAGACTGC TCTACACAAA GCAGCTGCCC AGGGTCATCT TGAAGTAGTC 
    CAGGCACTTC TGGAGTCAGG GGCTGAGATA GATATCACCA CTGATGATGG CGGGAACACT 
    CCACTTATTT ACTCTGTCGA AGGGGATGAA CCAGCTATCA CAAAGTATTT GATTGGTAAA 
    GGAGCGGATA TAAACAGAGG TAATGACAAC GGACGGAGAG CGATCCATCA AGCAGCTTAC 
    AAAGGGCTTG TTGACTGTGC AAGGGTCTTG ATTAATCATG GATGTGACGT TAATGTACAG 
    GATACAGAGA AAGATACTCC CCTCCATGAT GCCATAATGA AGAGCAGGTT AGATGTCATT 
    GAACTATTGG TGAAAGTTCC AGACCTCGAT GTTACTATTC CTGACGAGAG AGGGTTTACA 
    GCTCTACAGT TTGCAGCTTT AAAAGACAAA CCAGAAGCTG CTGAGTTAAT CGCGAGAAGT 
    TGCCCTCAAT CTATTGTTGT TGCAAAGGAT GATGGCTTCA ACATACTGCA TATCTGTGCA 
    GTCAATAACC ATGTAGAAGT CATGAAGGCT GTTATGGCTA TGAAGGATCA CGGGTTGGAT 
    GTGAATGCAA AGGAATCGCG AGGACTGACA GCACTACACC TGGCTGCTAA CGATGGATAT 
    TCCCATTGCA TAGAGTTTTT GATGTCACAG GGTGCTGATA TCAATCTTCG AGGTAAAGAT 
    GATCACACTG CCCTGCACAT CCTTGCCAGC ACTGCAGGGA AAGATCCAAG TGATATCAAA 
    GACACTCCTA CACTGAGGAA GATTCGCAAG CGTTTTAGAG ATTGTGGAGT TGATAACAGT 
    GCTGCCGCTG CACTCTGCTA CATGGTTCTT AAAGGAGCGT CCATAGATCT AGAGAATGTC 
    AAATGTAGAA CACCGCTATA TTACATCAGG AATGCTGAGC TGAAGAAGAC TTTGAAGAAG 
    ATTGCTCGTG AAAAAACATC TGACGATATG TCATCATGGG AGGACCTCGG GGAAGATGAG 
    GATGTGAAGA AGGGACGCCT GAGCAGTAAC TCCGTCAATG TTGATGACGA TGATATTATC 
    GTAAAGCGTA ACGATGGTCT GAGCGGGACA GACAAAGGCT CACCAAATCG AATAAGACAG 
    GACTCGGCTG AGAGTGAAAG TGGAGCTGAT GGTCCAGCAG TAAACCAAAA GAAAACTGAC 
    AAGCCAAAAA TTGAAGTCTT CGATCTTGTG CACAAAATTG AAGTCTCTGA CCTAGACATG 
    GGTCCTGTTG TTGGGAAGGG GCAGTTTGGA GTGGTCCATC GAGCCTCCTG GAGAGGGACT 
    CCAGTGGCAG TCAAAAAGAT CTCCATGATG GGTAGCAGAA AAGATGAAAT CGAGAAGGAA 
    GTCGCAATAC ACAGACGGGC ATGTCATCCT AACATCGTTC AGATGATGGC TCTAGGATAC 
    CAGGGAAATG AGGCCTTCCT GGTAATGCAG TTCATTGAAG GACCAAGTTT GCACACGGTC 
    ATTTTCCCTG ATAGGGATGA CGTTAAGATT CCTCTAGACT GGGCTAAACA GCTTCAAATA 
    TCTTGTGAGG TTCTCCAAGC CGTAACCTTC ATGCATGCCT GCTACATCTT ACATCTTGAT 
    ATTAAGCCTG CCAATATTCT AGTGGACTCT GCAACAATTC GTCCCTACAT CTGTGACTTG 
    GGCCTGGCCC ACATCAAGAA CCGCAACCTC ATGTCCCAAT CCGCAGTCAA CATGAGAGGC 
    ACACCGTGCT TCATGCCCCC AGAGGCACTT GGCGCAACGG AACCTGGCTA CAGGCACTCC 
    AACAAACATG ACATCTGGTC GGTTGCAGGA ACATTAGTGG AGTTGTACTC CATGAAGCGC 
    CTGTGGGGAG ACTCGATGAA TCCTCTGGTC CTGATGGCTC GTATTTATAC AGGCCAGATG 
    GGTAAACCAG ATTCTTTGAC AGCTGTAGAC CCTAAGGTTC AGAAGATCCT TGAACCATGC 
    TTCGAACAGA AGCCAGAGAA GAGACCATCA GCTTCTAATC TCCTGGATCA GTTTAATGAC 
    CTAATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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