LOC100893314 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100893314
Entrez Gene ID	100893314
Full Name	alpha-2-macroglobulin-like protein 1
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011677836.1	XP_011676138.1	alpha-2-macroglobulin-like protein 1 isoform X1
XM_011677837.1	XP_011676139.1	alpha-2-macroglobulin-like protein 1 isoform X2
XM_011677836.2	XP_011676138.2	alpha-2-macroglobulin-like protein 1

Pathways from BioSystems
Gene Ontology

The following LOC100893314 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100893314 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd72192	XM_011677836.2 Latest version!	Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSd32863	XM_011677836.1 Latest version!	Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSd32864	XM_011677837.1 Latest version!	Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100893314 ( XM_011677836.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011676138.2 Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), mRNA.

Price & Availability↑

CloneID	OSd72192
Clone ID Related Accession (Same CDS sequence)	XM_011677836.2
Accession Version	XM_011677836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	alpha-2-macroglobulin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_011677836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTA TCGTAAGTCT TCTCATCTTC ACTATCGCAG TGATGGTGAG AGGACAGGAA 
GATGGAGATG AACTGCGGGT GGCTCTCACT TTACCTCCAA TTGCCGAAGA AGTTACACAA 
CTACCCGAAC CGACTACAAC TCCAGCACTA CCACTAAGCC CTGAGGAACT CCGAGAAGAG 
GATTTCAACA AGGAAATCTC CTACCTCTTC CTAATCCCTA AACTCTTCTA CGTGCAAACC 
AAAGAGAGGA TATGTCTCCT GGTAACCAAG AGCGACGGTC TCCTGGACTA CCGGGTGTCT 
CTCCAGAAGA TAAATGCAGG TCAATACGAA GAATACTGGA GTCCTGGAGA ACAGGAGGCT 
AAACAAGGAG AGAACTGTTT CGAATTTGAT GTACCAACGA TCGAGTCGGG ATCCAATCAA 
AGACAGAAAG TTCGTCTTCA ACTGCGACGA AAGGGAGCCG AAGACTTGAA CGAGGACGGA 
TATGAAGTCA CGAAAGAGAG ATTCGTGAGG ATCCAGGCCC AAGGCGGGTC GAAAACTTAC 
GTCCAGACTG ACAAACCCAT TTACCAACCC GGTCAGACAG TGAGATTCCG TGCTCTGACA 
TTGAACAGTG AACTGAAAAC CGTCACAGAA CCATTCGATT CTGTTATAGT GGAGAGTCAG 
TCTCGCCAGC GCGTCAACCA GTGGACGGAC GTCAAGGGTG AGGAAGGGCT GATTGAACTC 
GAGCACGAAC TCCCGGACGA GCTCCCGGAC GGGATCACGA AACTGACTTA CTACATCAAG 
GTCTTGCGAG GCACCGAATC AGTAACGCAG ACATTTACTG TTGAAGAGTA CGTTCTTCCA 
AGGTTTGAGG TTGAAGTTAT AACACCTCCG TACATTCTCA TCACCGATCC TACTTGCACA 
GTCAAGGTTT GTGGAACTTA CACCTATGGT AAACCCGTTC AAGGATCTGT GCAAGCAAAT 
ATCACTGCTG ACGGGGGGCG ATACGACTAC AGGACTAGAA CTCGGTCTTC TAGAGAGGTT 
CTGCCGTTCG CCATACCGAG TACTGACGCC GATGGTTGTG CTGTTATCAA TGTTAATCTC 
ACACGCTTAG AGCTCACATC GAGAAAGTTC TATATCTATG GAAATCTGCA AGTATCCGCC 
AGGTTTACCG AAACGGCTAC AGGTGAAACG CTGACTGATA AATCTACGTC CCGTATGAGC 
TCATATACAC TAACCATGAG GGCTATAAAC AAAAACAATT TCTTCAAGCC TGCACTACCA 
TACTTTGGAG CGGTCGAGGT GACTAATCCC GATGGAGCCC CAAAGCCTGG TGAGAGCATT 
ATCGTGCGAT GCAATGCTTT GGATTCCCCT GTAGAAGCCA CTTCTAATGA CCAAGGATTC 
GCTGTGTTCA CTTTACCACT TCAGGGAATC TACGAAGGAT TCGATAGTGG AAACTCTCTC 
AGTTGCAATG TGACAGCCAA AGACTTCCAA GAGAACATTC CATATGACCC CGATGTCTGG 
ACCCTGAATC GACCAATCAC TTACTTCAGT CTGAGTCCTA TGTACTCCCC GACCGACAAC 
TATCTGGAGG TCGAAGCGGT GATCCAATTT GGCAATCCAA TACAAGTTGG CGGCAGCGTA 
TCTATTTCAG TCCACATGAC CAAGGATTGC ACTGATGGAC CCAAGATACT GGCTGTGTCT 
CGAGGGAACA TCCTCCAGGA TTTCGAATAC AAGGCATCCC GTCAGGAGAG CGCAAACGGC 
ATCATTTATG ACGTCACCTT TGTTCTCACG CCGTTAATGA CCCCGTTTCT CGACCTTCTA 
GTCTTTGGTA TCCTTGATGA CGGAGAGATG ATCGCCGATA GTCTGGAAAT CGAAAGCAAA 
GCCGTCTTTG AGAATGAGGT TCACATCCAG GTCGCCGACG GACAGGATGC CACACTCGAG 
CCGGGGGCAC GGACGAGCAT CGAGATCATA GCATCCCCCG GATCGTTGTG CGCTGTCGGA 
GTGGTCGATA AGAGCGTGCA TATTCTCGGA GGCAACAACC AGATGAAGAC CAGCGACGTG 
GTCAGCTTTC TGAACACATT CCAGTTGAGC TCAGGTGACA ATGATAATCC GCGGTGCCAG 
GATCACGAGG ACGAACCAAT CAATATTGAT GATGGCGGAG TTGGCGGCCC TATACCTTTT 
ATACCGCGGC CAGTATTTGA AGTAGCACTG GAACCCCAAG TATTAGCTCG CAAGAAGCGA 
CAGTTTGGTT TCTCACCGTA TCGTAGAGAT CCATATCCGT TATACTCCGA CGTGACCAGG 
GAATTTGAGC AACTGGGTCT TGTTCGGATT TCTAATCTTA AAGTCGAGAC GAGTCCATGT 
CAGACGCCAG AACCCTATCC ATATTACTTT CGGAATGAAG CGTTCGCGTT TGCTGCTCAG 
GCAGACTTTG CACAAGCACT TTCAGTTTCG GCGGTTTCAG CAGGTCCAGC CATTCGTAAA 
TATTTTCCAG AGACTTGGCT CTGGTATATG CAACGTCCAG AGGAATCCCC AACCGTCACC 
GTCCCTGATA CCATTACAGA GTGGGTTGGA AGTGGGTTCT GTATCTCCTC AACAACTGGC 
TTTGGTATCT CCGAACCCTT CTCAGTGACA GCTTTCAAAC AGTTCTTTGC GGATTACAAT 
CTCCCTTACT CTGTCATACG AGGCGAAGAG CTGGAGCTTC AAATTAACAT CTTCAACTAC 
TTGGAAAAGT GCTTACCGGT TAAGGTAACT CTCACTGCGT CTTCTAAATT TGAGATCAAA 
GAGGAATCCA ACAGCAAATA CACTTGCGTA TGTAGCAGCT CTGAACCAAC CCAAGTGACC 
TTTAACGTTC GGCCCACAGC ACTAGGGGAC GTTGATCTTG AAACAACCGT GATCGTTGCG 
TATGACCAGA CTGTATGCAG AGGGAAACCT GTTGAAACTG GTGTGACACA CAGTGATGGA 
CTAAGCAAGC CTATTCTCGT AGAGCCTGAA GGAGAGGAGG TGGAGGAATC ACTTAGTTTG 
TACTTCTGTC CAAAAGAAGA GGAGGACGGC ACATTCAGGC ATACATTTGA GATCTCAGTT 
CCGGTGGAGT ATGTTGCGGA CTCGGGCAGA GTTACCATCT TCATGTCAGG GGACATCCTC 
GGCAATAGTC TTGCGAACAT CGACAGACTG CTCAGGATCC CGTCTGGATG CGGTGAGCAG 
AACATGATCG GGTTTGTACC AAACATCTAC GCGCTCCAGT ACCTCCAAGC GACCGAACAA 
GCTGACCAAT CACAAGTCAA CAAAGCCTTG TCAAATATGA GGAAAGGATA CCAGAAGCAG 
CTCAATTACC GGCGTAGTCG TGGGTCTTAC TCGGCGTTTG GTAACAGGGA CCCAGCAGGT 
AGTGCATGGC TGACAGCCTA TGTGATTCGC TCCTTCGCTC AGGCCAGTCA ATTCATTTCC 
ATCGATACAA AGGACCTTGA CGTCAGTATT AATTGGCTGT ACAACACCCT CGGACAAGAG 
GAGAATGGGT GTTTCCGGTC AAGGGGTCGC GTAATCCACA AAGAGATGCA GGGTGGAGTG 
AATGATGAGG TGACTTTGAC AGCGTTCATA GTTGTCAGCT TACTGGAAGC CCAGCAGCCT 
TTGGAAACCC AGGCTATTCA AGACGCGTTG TCCTGTATCA CCAACTCTAT CGGCAGTTTG 
GAAGACACGT ACACTGCCGC CCTCATCGCC TACGCCCTGA GTATAGCTAA GGATACCAAC 
GTCGTTCAAG TCCTGGAATA CCTCGAATCA GTCGACAAAA ATGAAATTGG AGTGAGATAC 
TGGGTAAGCA GCCGAACGCA GACCCCGGGA TCGCGTAGGT ACGGCCGTGG TGCAACCGCT 
TCCTCCCTCA GCATTGAGAT GACAGCGTAC GTACTCCTAA CCTACATCAA TGTTTACGGT 
CATGACGCCA TAGCTCGCGG CTCGGCTATC TCCAAGTGGC TTGTCTCACA GCAAGGACCT 
CACGGAGGGT TTAAATCGTC TCAGGACACC GTCGTCGCCA TCCACGCTCT CGCTGAGTAT 
GCCCGTCTTG TGTTCGGCGG CGAGTCTAAC CTGGGCATTG CGTTCAGCTA CAAGGAGCAT 
TGTTTGTGGA ACTCAATATT CGTCATCCAT GATGGAAATC GTCTCGTTCT CCAGCGGGCC 
GACCTCGACC AACTCCCAAA GGCAATCGCA GTCATCGCTA CTGGCGAAGG ATGTGCCCTT 
CTCCAGGTGA TCATTCGATA TAACATTCAT CCGGCTCTCC CTCCCGCACC AGCCTTCGAG 
ATCATTTATA ACGTGGGTCC TACGACGCAA CAAGACGCCT GCCAGGGAAG CTTTAATCTC 
AATGTGTGTG TGAGGTATCT CGGTGATGAT GGATTTTCCA ACATGGCCAT GGTCGAGGTG 
AAGCTAGTTA CCGGTTTGTT CCTGAGCAAA ATTCCTTTAA TTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011676138.2
CDS	127..4554
Translation	MRLIVSLLIFTIAVMVRGQEDGDELRVALTLPPIAEEVTQLPEPTTTPALPLSPEELREEDFNKEISYLFLIPKLFYVQTKERICLLVTKSDGLLDYRVSLQKINAGQYEEYWSPGEQEAKQGENCFEFDVPTIESGSNQRQKVRLQLRRKGAEDLNEDGYEVTKERFVRIQAQGGSKTYVQTDKPIYQPGQTVRFRALTLNSELKTVTEPFDSVIVESQSRQRVNQWTDVKGEEGLIELEHELPDELPDGITKLTYYIKVLRGTESVTQTFTVEEYVLPRFEVEVITPPYILITDPTCTVKVCGTYTYGKPVQGSVQANITADGGRYDYRTRTRSSREVLPFAIPSTDADGCAVINVNLTRLELTSRKFYIYGNLQVSARFTETATGETLTDKSTSRMSSYTLTMRAINKNNFFKPALPYFGAVEVTNPDGAPKPGESIIVRCNALDSPVEATSNDQGFAVFTLPLQGIYEGFDSGNSLSCNVTAKDFQENIPYDPDVWTLNRPITYFSLSPMYSPTDNYLEVEAVIQFGNPIQVGGSVSISVHMTKDCTDGPKILAVSRGNILQDFEYKASRQESANGIIYDVTFVLTPLMTPFLDLLVFGILDDGEMIADSLEIESKAVFENEVHIQVADGQDATLEPGARTSIEIIASPGSLCAVGVVDKSVHILGGNNQMKTSDVVSFLNTFQLSSGDNDNPRCQDHEDEPINIDDGGVGGPIPFIPRPVFEVALEPQVLARKKRQFGFSPYRRDPYPLYSDVTREFEQLGLVRISNLKVETSPCQTPEPYPYYFRNEAFAFAAQADFAQALSVSAVSAGPAIRKYFPETWLWYMQRPEESPTVTVPDTITEWVGSGFCISSTTGFGISEPFSVTAFKQFFADYNLPYSVIRGEELELQINIFNYLEKCLPVKVTLTASSKFEIKEESNSKYTCVCSSSEPTQVTFNVRPTALGDVDLETTVIVAYDQTVCRGKPVETGVTHSDGLSKPILVEPEGEEVEESLSLYFCPKEEEDGTFRHTFEISVPVEYVADSGRVTIFMSGDILGNSLANIDRLLRIPSGCGEQNMIGFVPNIYALQYLQATEQADQSQVNKALSNMRKGYQKQLNYRRSRGSYSAFGNRDPAGSAWLTAYVIRSFAQASQFISIDTKDLDVSINWLYNTLGQEENGCFRSRGRVIHKEMQGGVNDEVTLTAFIVVSLLEAQQPLETQAIQDALSCITNSIGSLEDTYTAALIAYALSIAKDTNVVQVLEYLESVDKNEIGVRYWVSSRTQTPGSRRYGRGATASSLSIEMTAYVLLTYINVYGHDAIARGSAISKWLVSQQGPHGGFKSSQDTVVAIHALAEYARLVFGGESNLGIAFSYKEHCLWNSIFVIHDGNRLVLQRADLDQLPKAIAVIATGEGCALLQVIIRYNIHPALPPAPAFEIIYNVGPTTQQDACQGSFNLNVCVRYLGDDGFSNMAMVEVKLVTGLFLSKIPLIC

Target ORF information:

RefSeq Version	XM_011677836.2
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011677836.2

ORF Insert Sequence:

ATGAGGCTTA TCGTAAGTCT TCTCATCTTC ACTATCGCAG TGATGGTGAG AGGACAGGAA 
GATGGAGATG AACTGCGGGT GGCTCTCACT TTACCTCCAA TTGCCGAAGA AGTTACACAA 
CTACCCGAAC CGACTACAAC TCCAGCACTA CCACTAAGCC CTGAGGAACT CCGAGAAGAG 
GATTTCAACA AGGAAATCTC CTACCTCTTC CTAATCCCTA AACTCTTCTA CGTGCAAACC 
AAAGAGAGGA TATGTCTCCT GGTAACCAAG AGCGACGGTC TCCTGGACTA CCGGGTGTCT 
CTCCAGAAGA TAAATGCAGG TCAATACGAA GAATACTGGA GTCCTGGAGA ACAGGAGGCT 
AAACAAGGAG AGAACTGTTT CGAATTTGAT GTACCAACGA TCGAGTCGGG ATCCAATCAA 
AGACAGAAAG TTCGTCTTCA ACTGCGACGA AAGGGAGCCG AAGACTTGAA CGAGGACGGA 
TATGAAGTCA CGAAAGAGAG ATTCGTGAGG ATCCAGGCCC AAGGCGGGTC GAAAACTTAC 
GTCCAGACTG ACAAACCCAT TTACCAACCC GGTCAGACAG TGAGATTCCG TGCTCTGACA 
TTGAACAGTG AACTGAAAAC CGTCACAGAA CCATTCGATT CTGTTATAGT GGAGAGTCAG 
TCTCGCCAGC GCGTCAACCA GTGGACGGAC GTCAAGGGTG AGGAAGGGCT GATTGAACTC 
GAGCACGAAC TCCCGGACGA GCTCCCGGAC GGGATCACGA AACTGACTTA CTACATCAAG 
GTCTTGCGAG GCACCGAATC AGTAACGCAG ACATTTACTG TTGAAGAGTA CGTTCTTCCA 
AGGTTTGAGG TTGAAGTTAT AACACCTCCG TACATTCTCA TCACCGATCC TACTTGCACA 
GTCAAGGTTT GTGGAACTTA CACCTATGGT AAACCCGTTC AAGGATCTGT GCAAGCAAAT 
ATCACTGCTG ACGGGGGGCG ATACGACTAC AGGACTAGAA CTCGGTCTTC TAGAGAGGTT 
CTGCCGTTCG CCATACCGAG TACTGACGCC GATGGTTGTG CTGTTATCAA TGTTAATCTC 
ACACGCTTAG AGCTCACATC GAGAAAGTTC TATATCTATG GAAATCTGCA AGTATCCGCC 
AGGTTTACCG AAACGGCTAC AGGTGAAACG CTGACTGATA AATCTACGTC CCGTATGAGC 
TCATATACAC TAACCATGAG GGCTATAAAC AAAAACAATT TCTTCAAGCC TGCACTACCA 
TACTTTGGAG CGGTCGAGGT GACTAATCCC GATGGAGCCC CAAAGCCTGG TGAGAGCATT 
ATCGTGCGAT GCAATGCTTT GGATTCCCCT GTAGAAGCCA CTTCTAATGA CCAAGGATTC 
GCTGTGTTCA CTTTACCACT TCAGGGAATC TACGAAGGAT TCGATAGTGG AAACTCTCTC 
AGTTGCAATG TGACAGCCAA AGACTTCCAA GAGAACATTC CATATGACCC CGATGTCTGG 
ACCCTGAATC GACCAATCAC TTACTTCAGT CTGAGTCCTA TGTACTCCCC GACCGACAAC 
TATCTGGAGG TCGAAGCGGT GATCCAATTT GGCAATCCAA TACAAGTTGG CGGCAGCGTA 
TCTATTTCAG TCCACATGAC CAAGGATTGC ACTGATGGAC CCAAGATACT GGCTGTGTCT 
CGAGGGAACA TCCTCCAGGA TTTCGAATAC AAGGCATCCC GTCAGGAGAG CGCAAACGGC 
ATCATTTATG ACGTCACCTT TGTTCTCACG CCGTTAATGA CCCCGTTTCT CGACCTTCTA 
GTCTTTGGTA TCCTTGATGA CGGAGAGATG ATCGCCGATA GTCTGGAAAT CGAAAGCAAA 
GCCGTCTTTG AGAATGAGGT TCACATCCAG GTCGCCGACG GACAGGATGC CACACTCGAG 
CCGGGGGCAC GGACGAGCAT CGAGATCATA GCATCCCCCG GATCGTTGTG CGCTGTCGGA 
GTGGTCGATA AGAGCGTGCA TATTCTCGGA GGCAACAACC AGATGAAGAC CAGCGACGTG 
GTCAGCTTTC TGAACACATT CCAGTTGAGC TCAGGTGACA ATGATAATCC GCGGTGCCAG 
GATCACGAGG ACGAACCAAT CAATATTGAT GATGGCGGAG TTGGCGGCCC TATACCTTTT 
ATACCGCGGC CAGTATTTGA AGTAGCACTG GAACCCCAAG TATTAGCTCG CAAGAAGCGA 
CAGTTTGGTT TCTCACCGTA TCGTAGAGAT CCATATCCGT TATACTCCGA CGTGACCAGG 
GAATTTGAGC AACTGGGTCT TGTTCGGATT TCTAATCTTA AAGTCGAGAC GAGTCCATGT 
CAGACGCCAG AACCCTATCC ATATTACTTT CGGAATGAAG CGTTCGCGTT TGCTGCTCAG 
GCAGACTTTG CACAAGCACT TTCAGTTTCG GCGGTTTCAG CAGGTCCAGC CATTCGTAAA 
TATTTTCCAG AGACTTGGCT CTGGTATATG CAACGTCCAG AGGAATCCCC AACCGTCACC 
GTCCCTGATA CCATTACAGA GTGGGTTGGA AGTGGGTTCT GTATCTCCTC AACAACTGGC 
TTTGGTATCT CCGAACCCTT CTCAGTGACA GCTTTCAAAC AGTTCTTTGC GGATTACAAT 
CTCCCTTACT CTGTCATACG AGGCGAAGAG CTGGAGCTTC AAATTAACAT CTTCAACTAC 
TTGGAAAAGT GCTTACCGGT TAAGGTAACT CTCACTGCGT CTTCTAAATT TGAGATCAAA 
GAGGAATCCA ACAGCAAATA CACTTGCGTA TGTAGCAGCT CTGAACCAAC CCAAGTGACC 
TTTAACGTTC GGCCCACAGC ACTAGGGGAC GTTGATCTTG AAACAACCGT GATCGTTGCG 
TATGACCAGA CTGTATGCAG AGGGAAACCT GTTGAAACTG GTGTGACACA CAGTGATGGA 
CTAAGCAAGC CTATTCTCGT AGAGCCTGAA GGAGAGGAGG TGGAGGAATC ACTTAGTTTG 
TACTTCTGTC CAAAAGAAGA GGAGGACGGC ACATTCAGGC ATACATTTGA GATCTCAGTT 
CCGGTGGAGT ATGTTGCGGA CTCGGGCAGA GTTACCATCT TCATGTCAGG GGACATCCTC 
GGCAATAGTC TTGCGAACAT CGACAGACTG CTCAGGATCC CGTCTGGATG CGGTGAGCAG 
AACATGATCG GGTTTGTACC AAACATCTAC GCGCTCCAGT ACCTCCAAGC GACCGAACAA 
GCTGACCAAT CACAAGTCAA CAAAGCCTTG TCAAATATGA GGAAAGGATA CCAGAAGCAG 
CTCAATTACC GGCGTAGTCG TGGGTCTTAC TCGGCGTTTG GTAACAGGGA CCCAGCAGGT 
AGTGCATGGC TGACAGCCTA TGTGATTCGC TCCTTCGCTC AGGCCAGTCA ATTCATTTCC 
ATCGATACAA AGGACCTTGA CGTCAGTATT AATTGGCTGT ACAACACCCT CGGACAAGAG 
GAGAATGGGT GTTTCCGGTC AAGGGGTCGC GTAATCCACA AAGAGATGCA GGGTGGAGTG 
AATGATGAGG TGACTTTGAC AGCGTTCATA GTTGTCAGCT TACTGGAAGC CCAGCAGCCT 
TTGGAAACCC AGGCTATTCA AGACGCGTTG TCCTGTATCA CCAACTCTAT CGGCAGTTTG 
GAAGACACGT ACACTGCCGC CCTCATCGCC TACGCCCTGA GTATAGCTAA GGATACCAAC 
GTCGTTCAAG TCCTGGAATA CCTCGAATCA GTCGACAAAA ATGAAATTGG AGTGAGATAC 
TGGGTAAGCA GCCGAACGCA GACCCCGGGA TCGCGTAGGT ACGGCCGTGG TGCAACCGCT 
TCCTCCCTCA GCATTGAGAT GACAGCGTAC GTACTCCTAA CCTACATCAA TGTTTACGGT 
CATGACGCCA TAGCTCGCGG CTCGGCTATC TCCAAGTGGC TTGTCTCACA GCAAGGACCT 
CACGGAGGGT TTAAATCGTC TCAGGACACC GTCGTCGCCA TCCACGCTCT CGCTGAGTAT 
GCCCGTCTTG TGTTCGGCGG CGAGTCTAAC CTGGGCATTG CGTTCAGCTA CAAGGAGCAT 
TGTTTGTGGA ACTCAATATT CGTCATCCAT GATGGAAATC GTCTCGTTCT CCAGCGGGCC 
GACCTCGACC AACTCCCAAA GGCAATCGCA GTCATCGCTA CTGGCGAAGG ATGTGCCCTT 
CTCCAGGTGA TCATTCGATA TAACATTCAT CCGGCTCTCC CTCCCGCACC AGCCTTCGAG 
ATCATTTATA ACGTGGGTCC TACGACGCAA CAAGACGCCT GCCAGGGAAG CTTTAATCTC 
AATGTGTGTG TGAGGTATCT CGGTGATGAT GGATTTTCCA ACATGGCCAT GGTCGAGGTG 
AAGCTAGTTA CCGGTTTGTT CCTGAGCAAA ATTCCTTTAA TTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100893314 ( XM_011677836.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011676138.1 Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd32863
Clone ID Related Accession (Same CDS sequence)	XM_011677836.1
Accession Version	XM_011677836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	alpha-2-macroglobulin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011992034.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTA TCGTAAGTCT TCTCATCTTC ACTATCGCAG TGATGGTGAG AGGACAGGAA 
GATGGAGATG AACTGCGGGT GGCTCTCACT TTACCTCCAA TTGCCGAAGA AGTTACACAA 
CTACCCGAAC CGACTACAAC TCCAGCACTA CCACTAAGCC CTGAGGAACT CCGAGAAGAG 
GATTTCAACA AGGAAATCTC CTACCTCTTC CTAATCCCTA AACTCTTCTA CGTGCAAACC 
AAAGAGAGGA TATGTCTCCT GGTAACCAAG AGCGACGGTC TCCTGGACTA CCGGGTGTCT 
CTCCAGAAGA TAAATGCAGG TCAATACGAA GAATACTGGA GTCCTGGAGA ACAGGAGGCT 
AAACAAGGAG AGAACTGTTT CGAATTTGAT GTACCAACGA TCGAGTCGGG ATCCAATCAA 
AGACAGAAAG TTCGTCTTCA ACTGCGACGA AAGGGAGCCG AAGACTTGAA CGAGGACGGA 
TATGAAGTCA CGAAAGAGAG ATTCGTGAGG ATCCAGGCCC AAGGCGGGTC GAAAACATAC 
GTCCAGACTG ACAAACCCAT TTACCAACCC GGTCAGACAG TGAGATTCCG TGCTCTGACA 
TTGAACAGTG AACTGAAAAC CGTCACAGAA CCATTCGATT CTGTTATAGT GGAGAGTCAG 
TCTCGCCAGC GCGTCAACCA GTGGACGGAC GTCAAGGGTG AGGAAGGGCT GATTGAACTC 
GAGCACGAAC TCCCGGACGA GCTCCCGGAC GGGATCACGA AACTGACTTA CTACATCAAG 
GTCTTGCGAG GCACCGAATC AGTAACGCAG ACATTTACTG TTGAAGAGTA CGTTCTTCCA 
AGGTTTGAGG TTGAAGTTAT AACACCTCCG TACATTCTCA TCACCGATCC TACTTGCACA 
GTCAAGGTTT GTGGAACTTA CACGTATGGT AAACCCGTTC AAGGATCTGT GCAAGCAAAC 
ATCACTGCTG ACGGGGGGCG ATACGACTAC AGGACTAGAA CTCGGTCTTC TAGAGAGGTT 
CTACCGTTCG ACATACCGAG TACTGATGCC GATGGTTGTG CTGTTATCAA TGTTAATCTC 
ACACGCTTAG AGCTCACATC GAGAAAGTTC TATATCTATG GAAGTCTGCA AGTATCCGCC 
AGGTTTACCG AAACGGCTAC AGGTGAAACG CTGACTGATA AATCTACGTC GCGTATGAGC 
TCATCTACAC TCACCATGAG GGCTATAAAC AAAAACAATT TCTTCAAGCC TGCACTACCA 
TACTTTGGAG CGGTCAAGGT GACTAATCCC GATGGAGCCC CAAAGCCTGG TGAGAGCATT 
ATCGTGCGAT GCAATGCTTT GGCTTCACCT GTAGAAGCCA CTTCCAATGA CCAAGGATTC 
GCTGTATTCA CTTTACCACT TCAGGGAATC TACGAAGGAT TCGATAGTGG AAACTCTCTC 
AGCTGCAATG TGACAGCCAC AGATTTCCAA GAGAACATTC CATATGACCC CGATGTCTGG 
ACCCTGAATC GACCAATCAC TTACTTTAGT CTGAGTCCTA TGTACTCCCC GACCGACAAC 
TATCTGGAGG TCGAAGCGGT GATCCAATTT GGCAATCCAA TACAAGTTGG CGGCAGCGTA 
TCTATTTCAG TCCACATGAC CAAGGATTGC ATTGGTGGAC CCAGGATACT GGCTGTGTCT 
CGAGGGAACA TCCTCCAGGA TTTCGAATAC AAGGCATCCC GTCAGGAGAG CGCAAACGGC 
ATCATTTATG ACGTCACCTT TGTTCTCACG CCGTTAATGA CCCCGTTTCT CGACCTTCTA 
GTCTTTGGTA TCCTTGATGA CGGAGAGATG ATCGCCGATA GTCTGGAAAT CGAAAGCAAA 
GCCGTCTTTG AGAATGAGGT TCACATCCAG GTCGCCGACG GACAGGATGC TACACTCGAG 
CCGGGGGCAC GGACGAGCAT CGAGATCATA GCATCCCCCG GATCGTTGTG CGCTGTCGGA 
GTGGTCGATA AGAGCGTGCA TATTCTCGGA GGCAACAACC AGATGAAGAC CAGCGACGTG 
GTCAGCTTTC TGAACACATT CCAGTTGAGC TCAGGTGACA ATGATAATCC GCGGTGCCAG 
GATCACGAGG ACGAACCAAT CAATATTGAT GATGGCGGAG TTGGCGGCCC TATACCTTTT 
ATACCGCGGC CAGTATTTGA AGTAGCACTG GAACCCCAAG TATTAGCTCG CAAGAAGCGA 
CAGTTTGGTT TCTCACCGTA TCGTAGAGAT CCATATCCGT TATACTCCGA CGTGACCAGG 
GAATTTGAGC AACTGGGTCT TGTTCGGATT TCTAATCTTA AAGTCGAGAC GAGTCCATGT 
CAGACGCCAG AACCCTATCC ATATTACTTT CGGAATGAAG CGTTCGCGTT TGCTGCTCAG 
GCAGACTTTG CACAAGCACT TTCAGTTTCG GCGGTTTCAG CAGGTCCAGC CATTCGTAAA 
TATTTTCCAG AGACTTGGCT CTGGTATATG CAACGTCCAG AGGAATCCCC AACCGTCACC 
GTCCCTGATA CCATTACAGA GTGGGTTGGA AGTGGGTTCT GTATCTCCTC AACAACTGGC 
TTTGGTATCT CCGAACCCTT CTCAGTGACA GCTTTCAAAC AGTTCTTTGC GGATTACAAT 
CTCCCTTACT CTGTCATACG AGGCGAAGAG CTGGAGCTTC AAATTAACAT CTTCAACTAC 
TTGGAAAAGT GCTTACCGGT TAAGGTAACT CTCACTGCGT CTTCTAAATT TGAGATCAAA 
GAGGAATCCA ACAGCAAATA CACTTGCGTA TGTAGCAGCT CTGAACCAAC CCAAGTGACC 
TTTAACGTTC GGCCCACAGC ACTAGGGGAC GTTGATCTTG AAACAACCGT GATCGTTGCG 
TATGACCAGA CTGTATGCAG AGGGAAACCT GTTGAAACTG GTGTGACACA CAGTGATGGA 
CTAAGCAAGC CTATTCTCGT AGAGCCTGAA GGAGAGGAGG TGGAGGAATC ACTTAGTTTG 
TACTTCTGTC CAAAAGAAGA GGAGGACGGC ACATTCAGGT ATACATTTGA GATCTCAGTT 
CCGGTGGAGT ATGTTGCGGA CTCGGGCAGA GTTACCATCT TCATGTCAGG GGACATCCTC 
GGCAATAGTC TTGCGAACAT CGACAGACTG CTCAGGATCC CGTCTGGATG CGGTGAGCAG 
AACATGATCG GGTTTGTACC AAACATCTAC GCGCTCCAGT ACCTCCAAGC GACCGAACAA 
GCTGACCAAT CACAAGTCAA CAAAGCCTTG TCAAATATGA GGAAAGGATA CCAGAAGCAG 
CTCAATTACC GGCGTAGTCG CGGGTCTTAC TCGGCGTTTG GTAACAGGGA CCCAGCAGGT 
AGTGCATGGC TGACAGCCTA TGTGATTCGC TCCTTCGCTC AGGCCAGTCA ATTCATTTCC 
ATCGATACAA AGGACCTTGA CGTCAGTATT AATTGGCTGT ACAACACCCT CGGACAAGAG 
GAGAATGGGT GTTTCCGGTC AAGGGGTCGC GTAATCCACA AAGAGATGCA GGGTGGAGTG 
AATGATGAGG TGACTTTGAC AGCGTTCATA GTTGTCAGCT TACTGGAAGC CCAGCAGCCT 
TTGGAAACCC AGGCTATTCA AGACGCGTTG TCCTGTATCA CCAACTCTAT CGGCAGTTTG 
GAAGACACGT ACACTGCCGC CCTCATCGCC TACGCCCTGA GTATAGCTAA GGATACCAAC 
GTCGTTCAAG TCCTGGAATA CCTCGAATCA GTCGACAAAA ATGAAATTGG AGTGAGATAC 
TGGGTAAGCA GCCGAACGCA GACCCCGGGA TCGCGTAGGT ACGGCCGTGG TGCAACCGCT 
TCCTCCCTCA GCATTGAGAT GACAGCGTAC GTACTCCTAA CCTACATCAA TGTTTACGGT 
CATGACGCCA TAGCTCGCGG CTCGGCTATC TCCAAGTGGC TTGTCTCACA GCAAGGACCT 
CACGGAGGGT TTAAATCGTC TCAGGACACC GTCGTCGCCA TCCACGCTCT CGCTGAGTAT 
GCCCGTCTTG TGTTCGGCGG CGAGTCTAAC CTGGGCATTG CGTTCAGCTA CAAGGAGCAT 
TGTTTGTGGA ACTCAATATT CGTCATCCAT GATGGAAATC GTCTCGTTCT CCAGCGGGCC 
GACCTCGACC AACTCCCAAA GGCAATCGCA ATGATCGCTA CTGGCGAAGG ATGTGCCCTT 
CTCCAGGTGA TCATTCGATA TAACATTCAT CCGGCTCTCC CTCCCGCACC AGCCTTCGAG 
ATCATTTATA ACGTGGGTCC TACGACGCAA CAAGACGCCT GCCAGGGAAG CTTTAATCTC 
AATGTGTGTG TGAGGTATCT CGGTGATGAT GGATTTTCCA ACATGGCCAT GGTCGAGGTG 
AAGCTAGTTA CCGGTTTCGT TCCTGAGCAA AATTCCTTTA ATTTGCTGAG GAATGGGTTC 
TACCCAGAAC TCACTAGGGC TGAATATACA GGCAAGACGG TCGCCATGTA TTTCAACCAG 
TTTACCGCCA CACTGTTGTG CTTTGATCTG GAGGTTAGAC GTGTCCATCA CGTCACAGAT 
CCCAAGCCCG GTCTCATCAC GGTTTACGAC TACTACGCGC CAGATGTCAT TCTCTCAACC 
GAGTACAGCA TGGCTTGTCA ATCGAATGGT CCACCTATCA AAGTCCTACC GGTCGAAGAG 
CCCGAGAGGC CTATCCAAAT CCTACCGGTC GAAGCACTAC CAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011676138.1
CDS	106..4833
Translation	MRLIVSLLIFTIAVMVRGQEDGDELRVALTLPPIAEEVTQLPEPTTTPALPLSPEELREEDFNKEISYLFLIPKLFYVQTKERICLLVTKSDGLLDYRVSLQKINAGQYEEYWSPGEQEAKQGENCFEFDVPTIESGSNQRQKVRLQLRRKGAEDLNEDGYEVTKERFVRIQAQGGSKTYVQTDKPIYQPGQTVRFRALTLNSELKTVTEPFDSVIVESQSRQRVNQWTDVKGEEGLIELEHELPDELPDGITKLTYYIKVLRGTESVTQTFTVEEYVLPRFEVEVITPPYILITDPTCTVKVCGTYTYGKPVQGSVQANITADGGRYDYRTRTRSSREVLPFDIPSTDADGCAVINVNLTRLELTSRKFYIYGSLQVSARFTETATGETLTDKSTSRMSSSTLTMRAINKNNFFKPALPYFGAVKVTNPDGAPKPGESIIVRCNALASPVEATSNDQGFAVFTLPLQGIYEGFDSGNSLSCNVTATDFQENIPYDPDVWTLNRPITYFSLSPMYSPTDNYLEVEAVIQFGNPIQVGGSVSISVHMTKDCIGGPRILAVSRGNILQDFEYKASRQESANGIIYDVTFVLTPLMTPFLDLLVFGILDDGEMIADSLEIESKAVFENEVHIQVADGQDATLEPGARTSIEIIASPGSLCAVGVVDKSVHILGGNNQMKTSDVVSFLNTFQLSSGDNDNPRCQDHEDEPINIDDGGVGGPIPFIPRPVFEVALEPQVLARKKRQFGFSPYRRDPYPLYSDVTREFEQLGLVRISNLKVETSPCQTPEPYPYYFRNEAFAFAAQADFAQALSVSAVSAGPAIRKYFPETWLWYMQRPEESPTVTVPDTITEWVGSGFCISSTTGFGISEPFSVTAFKQFFADYNLPYSVIRGEELELQINIFNYLEKCLPVKVTLTASSKFEIKEESNSKYTCVCSSSEPTQVTFNVRPTALGDVDLETTVIVAYDQTVCRGKPVETGVTHSDGLSKPILVEPEGEEVEESLSLYFCPKEEEDGTFRYTFEISVPVEYVADSGRVTIFMSGDILGNSLANIDRLLRIPSGCGEQNMIGFVPNIYALQYLQATEQADQSQVNKALSNMRKGYQKQLNYRRSRGSYSAFGNRDPAGSAWLTAYVIRSFAQASQFISIDTKDLDVSINWLYNTLGQEENGCFRSRGRVIHKEMQGGVNDEVTLTAFIVVSLLEAQQPLETQAIQDALSCITNSIGSLEDTYTAALIAYALSIAKDTNVVQVLEYLESVDKNEIGVRYWVSSRTQTPGSRRYGRGATASSLSIEMTAYVLLTYINVYGHDAIARGSAISKWLVSQQGPHGGFKSSQDTVVAIHALAEYARLVFGGESNLGIAFSYKEHCLWNSIFVIHDGNRLVLQRADLDQLPKAIAMIATGEGCALLQVIIRYNIHPALPPAPAFEIIYNVGPTTQQDACQGSFNLNVCVRYLGDDGFSNMAMVEVKLVTGFVPEQNSFNLLRNGFYPELTRAEYTGKTVAMYFNQFTATLLCFDLEVRRVHHVTDPKPGLITVYDYYAPDVILSTEYSMACQSNGPPIKVLPVEEPERPIQILPVEALPE

Target ORF information:

RefSeq Version	XM_011677836.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011677836.1

ORF Insert Sequence:

ATGAGGCTTA TCGTAAGTCT TCTCATCTTC ACTATCGCAG TGATGGTGAG AGGACAGGAA 
GATGGAGATG AACTGCGGGT GGCTCTCACT TTACCTCCAA TTGCCGAAGA AGTTACACAA 
CTACCCGAAC CGACTACAAC TCCAGCACTA CCACTAAGCC CTGAGGAACT CCGAGAAGAG 
GATTTCAACA AGGAAATCTC CTACCTCTTC CTAATCCCTA AACTCTTCTA CGTGCAAACC 
AAAGAGAGGA TATGTCTCCT GGTAACCAAG AGCGACGGTC TCCTGGACTA CCGGGTGTCT 
CTCCAGAAGA TAAATGCAGG TCAATACGAA GAATACTGGA GTCCTGGAGA ACAGGAGGCT 
AAACAAGGAG AGAACTGTTT CGAATTTGAT GTACCAACGA TCGAGTCGGG ATCCAATCAA 
AGACAGAAAG TTCGTCTTCA ACTGCGACGA AAGGGAGCCG AAGACTTGAA CGAGGACGGA 
TATGAAGTCA CGAAAGAGAG ATTCGTGAGG ATCCAGGCCC AAGGCGGGTC GAAAACATAC 
GTCCAGACTG ACAAACCCAT TTACCAACCC GGTCAGACAG TGAGATTCCG TGCTCTGACA 
TTGAACAGTG AACTGAAAAC CGTCACAGAA CCATTCGATT CTGTTATAGT GGAGAGTCAG 
TCTCGCCAGC GCGTCAACCA GTGGACGGAC GTCAAGGGTG AGGAAGGGCT GATTGAACTC 
GAGCACGAAC TCCCGGACGA GCTCCCGGAC GGGATCACGA AACTGACTTA CTACATCAAG 
GTCTTGCGAG GCACCGAATC AGTAACGCAG ACATTTACTG TTGAAGAGTA CGTTCTTCCA 
AGGTTTGAGG TTGAAGTTAT AACACCTCCG TACATTCTCA TCACCGATCC TACTTGCACA 
GTCAAGGTTT GTGGAACTTA CACGTATGGT AAACCCGTTC AAGGATCTGT GCAAGCAAAC 
ATCACTGCTG ACGGGGGGCG ATACGACTAC AGGACTAGAA CTCGGTCTTC TAGAGAGGTT 
CTACCGTTCG ACATACCGAG TACTGATGCC GATGGTTGTG CTGTTATCAA TGTTAATCTC 
ACACGCTTAG AGCTCACATC GAGAAAGTTC TATATCTATG GAAGTCTGCA AGTATCCGCC 
AGGTTTACCG AAACGGCTAC AGGTGAAACG CTGACTGATA AATCTACGTC GCGTATGAGC 
TCATCTACAC TCACCATGAG GGCTATAAAC AAAAACAATT TCTTCAAGCC TGCACTACCA 
TACTTTGGAG CGGTCAAGGT GACTAATCCC GATGGAGCCC CAAAGCCTGG TGAGAGCATT 
ATCGTGCGAT GCAATGCTTT GGCTTCACCT GTAGAAGCCA CTTCCAATGA CCAAGGATTC 
GCTGTATTCA CTTTACCACT TCAGGGAATC TACGAAGGAT TCGATAGTGG AAACTCTCTC 
AGCTGCAATG TGACAGCCAC AGATTTCCAA GAGAACATTC CATATGACCC CGATGTCTGG 
ACCCTGAATC GACCAATCAC TTACTTTAGT CTGAGTCCTA TGTACTCCCC GACCGACAAC 
TATCTGGAGG TCGAAGCGGT GATCCAATTT GGCAATCCAA TACAAGTTGG CGGCAGCGTA 
TCTATTTCAG TCCACATGAC CAAGGATTGC ATTGGTGGAC CCAGGATACT GGCTGTGTCT 
CGAGGGAACA TCCTCCAGGA TTTCGAATAC AAGGCATCCC GTCAGGAGAG CGCAAACGGC 
ATCATTTATG ACGTCACCTT TGTTCTCACG CCGTTAATGA CCCCGTTTCT CGACCTTCTA 
GTCTTTGGTA TCCTTGATGA CGGAGAGATG ATCGCCGATA GTCTGGAAAT CGAAAGCAAA 
GCCGTCTTTG AGAATGAGGT TCACATCCAG GTCGCCGACG GACAGGATGC TACACTCGAG 
CCGGGGGCAC GGACGAGCAT CGAGATCATA GCATCCCCCG GATCGTTGTG CGCTGTCGGA 
GTGGTCGATA AGAGCGTGCA TATTCTCGGA GGCAACAACC AGATGAAGAC CAGCGACGTG 
GTCAGCTTTC TGAACACATT CCAGTTGAGC TCAGGTGACA ATGATAATCC GCGGTGCCAG 
GATCACGAGG ACGAACCAAT CAATATTGAT GATGGCGGAG TTGGCGGCCC TATACCTTTT 
ATACCGCGGC CAGTATTTGA AGTAGCACTG GAACCCCAAG TATTAGCTCG CAAGAAGCGA 
CAGTTTGGTT TCTCACCGTA TCGTAGAGAT CCATATCCGT TATACTCCGA CGTGACCAGG 
GAATTTGAGC AACTGGGTCT TGTTCGGATT TCTAATCTTA AAGTCGAGAC GAGTCCATGT 
CAGACGCCAG AACCCTATCC ATATTACTTT CGGAATGAAG CGTTCGCGTT TGCTGCTCAG 
GCAGACTTTG CACAAGCACT TTCAGTTTCG GCGGTTTCAG CAGGTCCAGC CATTCGTAAA 
TATTTTCCAG AGACTTGGCT CTGGTATATG CAACGTCCAG AGGAATCCCC AACCGTCACC 
GTCCCTGATA CCATTACAGA GTGGGTTGGA AGTGGGTTCT GTATCTCCTC AACAACTGGC 
TTTGGTATCT CCGAACCCTT CTCAGTGACA GCTTTCAAAC AGTTCTTTGC GGATTACAAT 
CTCCCTTACT CTGTCATACG AGGCGAAGAG CTGGAGCTTC AAATTAACAT CTTCAACTAC 
TTGGAAAAGT GCTTACCGGT TAAGGTAACT CTCACTGCGT CTTCTAAATT TGAGATCAAA 
GAGGAATCCA ACAGCAAATA CACTTGCGTA TGTAGCAGCT CTGAACCAAC CCAAGTGACC 
TTTAACGTTC GGCCCACAGC ACTAGGGGAC GTTGATCTTG AAACAACCGT GATCGTTGCG 
TATGACCAGA CTGTATGCAG AGGGAAACCT GTTGAAACTG GTGTGACACA CAGTGATGGA 
CTAAGCAAGC CTATTCTCGT AGAGCCTGAA GGAGAGGAGG TGGAGGAATC ACTTAGTTTG 
TACTTCTGTC CAAAAGAAGA GGAGGACGGC ACATTCAGGT ATACATTTGA GATCTCAGTT 
CCGGTGGAGT ATGTTGCGGA CTCGGGCAGA GTTACCATCT TCATGTCAGG GGACATCCTC 
GGCAATAGTC TTGCGAACAT CGACAGACTG CTCAGGATCC CGTCTGGATG CGGTGAGCAG 
AACATGATCG GGTTTGTACC AAACATCTAC GCGCTCCAGT ACCTCCAAGC GACCGAACAA 
GCTGACCAAT CACAAGTCAA CAAAGCCTTG TCAAATATGA GGAAAGGATA CCAGAAGCAG 
CTCAATTACC GGCGTAGTCG CGGGTCTTAC TCGGCGTTTG GTAACAGGGA CCCAGCAGGT 
AGTGCATGGC TGACAGCCTA TGTGATTCGC TCCTTCGCTC AGGCCAGTCA ATTCATTTCC 
ATCGATACAA AGGACCTTGA CGTCAGTATT AATTGGCTGT ACAACACCCT CGGACAAGAG 
GAGAATGGGT GTTTCCGGTC AAGGGGTCGC GTAATCCACA AAGAGATGCA GGGTGGAGTG 
AATGATGAGG TGACTTTGAC AGCGTTCATA GTTGTCAGCT TACTGGAAGC CCAGCAGCCT 
TTGGAAACCC AGGCTATTCA AGACGCGTTG TCCTGTATCA CCAACTCTAT CGGCAGTTTG 
GAAGACACGT ACACTGCCGC CCTCATCGCC TACGCCCTGA GTATAGCTAA GGATACCAAC 
GTCGTTCAAG TCCTGGAATA CCTCGAATCA GTCGACAAAA ATGAAATTGG AGTGAGATAC 
TGGGTAAGCA GCCGAACGCA GACCCCGGGA TCGCGTAGGT ACGGCCGTGG TGCAACCGCT 
TCCTCCCTCA GCATTGAGAT GACAGCGTAC GTACTCCTAA CCTACATCAA TGTTTACGGT 
CATGACGCCA TAGCTCGCGG CTCGGCTATC TCCAAGTGGC TTGTCTCACA GCAAGGACCT 
CACGGAGGGT TTAAATCGTC TCAGGACACC GTCGTCGCCA TCCACGCTCT CGCTGAGTAT 
GCCCGTCTTG TGTTCGGCGG CGAGTCTAAC CTGGGCATTG CGTTCAGCTA CAAGGAGCAT 
TGTTTGTGGA ACTCAATATT CGTCATCCAT GATGGAAATC GTCTCGTTCT CCAGCGGGCC 
GACCTCGACC AACTCCCAAA GGCAATCGCA ATGATCGCTA CTGGCGAAGG ATGTGCCCTT 
CTCCAGGTGA TCATTCGATA TAACATTCAT CCGGCTCTCC CTCCCGCACC AGCCTTCGAG 
ATCATTTATA ACGTGGGTCC TACGACGCAA CAAGACGCCT GCCAGGGAAG CTTTAATCTC 
AATGTGTGTG TGAGGTATCT CGGTGATGAT GGATTTTCCA ACATGGCCAT GGTCGAGGTG 
AAGCTAGTTA CCGGTTTCGT TCCTGAGCAA AATTCCTTTA ATTTGCTGAG GAATGGGTTC 
TACCCAGAAC TCACTAGGGC TGAATATACA GGCAAGACGG TCGCCATGTA TTTCAACCAG 
TTTACCGCCA CACTGTTGTG CTTTGATCTG GAGGTTAGAC GTGTCCATCA CGTCACAGAT 
CCCAAGCCCG GTCTCATCAC GGTTTACGAC TACTACGCGC CAGATGTCAT TCTCTCAACC 
GAGTACAGCA TGGCTTGTCA ATCGAATGGT CCACCTATCA AAGTCCTACC GGTCGAAGAG 
CCCGAGAGGC CTATCCAAAT CCTACCGGTC GAAGCACTAC CAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100893314 ( XM_011677837.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011676139.1 Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), transcript variant X2, mRNA.

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CloneID	OSd32864
Clone ID Related Accession (Same CDS sequence)	XM_011677837.1
Accession Version	XM_011677837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	alpha-2-macroglobulin-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011992034.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTTA TCGTAAGTCT TCTCATCTTC ACTATCGCAG TGATGGTGAG AGGACAGGAA 
GATGGAGATG AACTGCGGGT GGCTCTCACT TTACCTCCAA TTGCCGAAGA AGTTACACAA 
CTACCCGAAC CGACTACAAC TCCAGCACTA CCACTAAGCC CTGAGGAACT CCGAGAAGAG 
GATTTCAACA AGGAAATCTC CTACCTCTTC CTAATCCCTA AACTCTTCTA CGTGCAAACC 
AAAGAGAGGA TATGTCTCCT GGTAACCAAG AGCGACGGTC TCCTGGACTA CCGGGTGTCT 
CTCCAGAAGA TAAATGCAGG TCAATACGAA GAATACTGGA GTCCTGGAGA ACAGGAGGCT 
AAACAAGGAG AGAACTGTTT CGAATTTGAT GTACCAACGA TCGAGTCGGG ATCCAATCAA 
AGACAGAAAG TTCGTCTTCA ACTGCGACGA AAGGGAGCCG AAGACTTGAA CGAGGACGGA 
TATGAAGTCA CGAAAGAGAG ATTCGTGAGG ATCCAGGCCC AAGGCGGGTC GAAAACATAC 
GTCCAGACTG ACAAACCCAT TTACCAACCC GGTCAGACAG TGAGATTCCG TGCTCTGACA 
TTGAACAGTG AACTGAAAAC CGTCACAGAA CCATTCGATT CTGTTATAGT GGAGAGTCAG 
TCTCGCCAGC GCGTCAACCA GTGGACGGAC GTCAAGGGTG AGGAAGGGCT GATTGAACTC 
GAGCACGAAC TCCCGGACGA GCTCCCGGAC GGGATCACGA AACTGACTTA CTACATCAAG 
GTCTTGCGAG GCACCGAATC AGTAACGCAG ACATTTACTG TTGAAGAGTA CGTTCTTCCA 
AGGTTTGAGG TTGAAGTTAT AACACCTCCG TACATTCTCA TCACCGATCC TACTTGCACA 
GTCAAGGTTT GTGGAACTTA CACGTATGGT AAACCCGTTC AAGGATCTGT GCAAGCAAAC 
ATCACTGCTG ACGGGGGGCG ATACGACTAC AGGACTAGAA CTCGGTCTTC TAGAGAGGTT 
CTACCGTTCG ACATACCGAG TACTGATGCC GATGGTTGTG CTGTTATCAA TGTTAATCTC 
ACACGCTTAG AGCTCACATC GAGAAAGTTC TATATCTATG GAAGTCTGCA AGTATCCGCC 
AGGTTTACCG AAACGGCTAC AGGTGAAACG CTGACTGATA AATCTACGTC GCGTATGAGC 
TCATCTACAC TCACCATGAG GGCTATAAAC AAAAACAATT TCTTCAAGCC TGCACTACCA 
TACTTTGGAG CGGTCAAGGT GACTAATCCC GATGGAGCCC CAAAGCCTGG TGAGAGCATT 
ATCGTGCGAT GCAATGCTTT GGCTTCACCT GTAGAAGCCA CTTCCAATGA CCAAGGATTC 
GCTGTATTCA CTTTACCACT TCAGGGAATC TACGAAGGAT TCGATAGTGG AAACTCTCTC 
AGCTGCAATG TGACAGCCAA AGACTTCCAA GAGAACATTC CATATGACCC CGATGTCCGG 
ACCCTGAATC GACCAATCAC TTACTTCAGT CTGAGTCCTA TGTACTCCCC GACCGATAAC 
TATCTGGAGG TCGAAGCGGT GATCCAATTT GGCAATCCAA TACAAGTTGG CGGCAGCGTA 
TTTATTTCAG TCCACATGAC CAAGGATTGC ACTGGTGGAC CCAGGATACT GGCTGTGTCT 
CGGGGGAACA TCCTCCAGGA TTTCGAATAC GAGGCATCCC GTCAGGAGAA CGCAAACGGC 
ATCATTTATG ACGTCACCTT TGTTCTCACG CCGTTAATGA CCCCGTTTCT TGACCTCCTG 
GTCTATGGTA TCCTGGATGA CGGAGAGATG ATCGCCGATA GTCTCGAAAT CGAAAGCAAC 
GCCGTCTTTG AGAATGAGGT TCACATCCAG GTCGCCGAAG GACAGGATGC TACACTCGAG 
CCGGGGGCAC GGACGAGCAT CGAGATCATA GCATCCCCCG GATCGTTGTG CGCTGTCGGA 
GTGGTCGATA AGAGCGTGCA TATTCTAGGA GGCAACAACC AGATGAAGAC CAGCGACGTG 
GTCAGTTTTC TAAACACATT CCAGTTGAGG TCAGGTGACA ATGATAATCC GCGGTGCCAG 
GATCACGAGG ACGAACCAAT CAAGATTGAT GATGGCTTGG TTTGGTTTGG TTTCTCTCCG 
TATCGTAGAG ATCCATATCC GTTATACTCG GACGTGACCA AGGAATTTGA GCAACTGGGT 
CTTGTTCGGA TTTCTAATCT TAAAGTCGAG ACGAGTCCAT GTCAGACGCC AGAACCCTAT 
CCATATTACG CTCGGGGTCA GGTGCTTGAG AGAGCTGATT CTGAGCCAGA CTTTGCACAA 
GCATTTTCAG TTTCGGCGGC TTCAGCAGGT CCAGCCATTC GCAAATATTT TCCAGAGACT 
TGGCTCTGGT CTATGCAACG CCCAGAGGAA TCCCCAACCG TCACCGTCCC TGATACCATT 
ACAGAGTGGG TTGGAAGTGG GTTCTGTATC TCCTCAACGA CTGGCTTTGG TATCTCCAAA 
CCCTTCTCGG TGACAGCTTT CAAACCGTTC TTTGCGGATT ACAATCTCCC TTACTCTGTC 
ATACGAGGCG AAGAGCTGGA GCTTCAAATT AACATCTTCA ACTACTTGGA AAAGTGCTTA 
CCGGTTGAGG TAACTCTGAC TCCGTCTGCT AAATTTGAGA TCAAAGAGAA ATCCAACAGC 
AAATACACTT GCGTATGCAG CAGCTCTGAA CCAACCCAAG TGACCTTTAA CGTTCGGCCC 
ACAGCATTAG GGGACGTTGA TCTGGAAACA ACTGTGATCG TTGTGTATGA CCAGACTGTA 
TGCGGAGAGA AACCTGTTGA AACTGATGTA ACACACAGTG ATGGACTAAG CAAACCTATT 
CTCGTAGAGC CTGAAGGAGA GGAGGTGGAG GAATCGCTTA GTTTGTACTT CTGTCCAAAA 
GAAGAGAAAG GCGGCACATT CAGGCATACA TTTGAGATCT CAGTTCCGGT GGAGTATGTT 
ACGGACTCGG GCAGAGTTAC TATATTCTTG TCAGGGGACA TTCTCGGCAA TAGTCTTGCG 
AACATCGACA GACTGCTCAG GATCCCGTCT GGATGCGGTG AGCAGAACAT GATCGGGTTT 
GTACCAAACA TCTACGCGCT CCAGTACCTC CAAGCGACCG AACAAGCTGA CCAATCACAA 
GTCAACAAAG CCTTGTCAAA TATGAGGAAA GGATACCAGA AGCAGCTCAA TTACCGGCGT 
AGCCTCGGGT CTTACTCAGC GTTTGGTAAC CGGGACCCAT CAGGGAGTGC ATGGCTGACA 
GCCTATGTGA TTCGCTCCTT CGCTCAGGCC AGTCCATTCA TTACCATCGA TACAAAGGAC 
CTTGACGTCA GTATTAATTG GCTGTACAAC ACCCTCGGAC AAGAGGAGAA TGGGTGTTTC 
CGGTCAAGGG GTCGCGTAAT CCACAAAGAG ATGCAGGGTG GAGTGAATGA TGAGGTGACT 
TTGACAGCGT TCATAGTCGT TAGCTTACTG GAAGCCCAGC AGCCTTTGGA AACCCAGGCT 
ATCCAAGACG CGTTGTCCTG TATCCGCAAC TCTATCGGCA GTTTGGAAGA CACATACACT 
GCCGCCCTCA TCGCCTACGC CCTGAGTATA GCTAAGGATC CCAACGTCAA TAAAGTTCTG 
GAATACCTCG AATCAGTCGC CAAAAATGAA AATGGAGTGA GATACTGGGT AAGCAGCCGA 
ACCCAGACTC CGGGATCGCG TAGATACAAC CGTGGTGCAA CCGCTTCCTC CCTCAGCATT 
GAGATGACCG CGTACGTACT CCTAACCTAC ATCAATGTTT ACGGTCATGA CGCCATAGCC 
CGCGGCTCGG CTATCTCCAA GTGGATTGTC TCACAGCAAG GCCCTAACGG AGGGTTTAAA 
TCGTCTCAGG ACACCGTCGT CGCCATTCAC GCTCTCGCTG AGTATGCCCG TCTTGTGTTC 
GGCGGCGAGT CTAACCTGGG TATTGCGTTT AGCTACCAGG AGCCTTTTTC GTTGAACTCA 
ATATTCATCA TCCATGATGG AAATCGTCTC GTTCTCCAGC GGGCCGACCT CGACCAACTT 
CCAAAGGCCA TCGACGTCAT CGCTATTGGC GAAGGATGTG CCCTTCTTCA GGTTATCATC 
CGATATAACG TTCACCCGGC TCTTCCTCCC GCACCAGCCT TCGACATCAG TTTTAACGTG 
GGTCCAACGA CGCAACAACT TGAAGACGCC TGCCAAGAAA GCTTTAATCT CAATGTGTGT 
GTGAGGTATC TCGGTGATGA TGGATTTTCC AACATGGCCA TGGTCGAGGT GAAGCTAGTT 
ACTGGTTTCG TTCCTGAACA AAAGTCCTTT GATTTACTGA AGAATGGGTT CTACCCAGAA 
CTCACTAGGG CTGAACAAAG AGACAAGAAG GTCGTCTTGT ACTTCAACGA GTTTAACGCC 
AAACCGTTGT GCTTTGATCT GGAGGTTAGA CGTGTCCATC ACGTCACAGA TCCCAAGCCC 
GGTCTCATCA CGGTTTACGA CTACTACGCG CCAGATGTCA TTCTCTCCAC CGAGTGGCAC 
ATGGAATGTG TAATCGAAGC GAAAGGTGTA CCTCTTCAAC TACCTGCGGT CGAAGAGCCT 
GAGGAGCTAC CACCCGGCGA CCAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011676139.1
CDS	106..4755
Translation	MRLIVSLLIFTIAVMVRGQEDGDELRVALTLPPIAEEVTQLPEPTTTPALPLSPEELREEDFNKEISYLFLIPKLFYVQTKERICLLVTKSDGLLDYRVSLQKINAGQYEEYWSPGEQEAKQGENCFEFDVPTIESGSNQRQKVRLQLRRKGAEDLNEDGYEVTKERFVRIQAQGGSKTYVQTDKPIYQPGQTVRFRALTLNSELKTVTEPFDSVIVESQSRQRVNQWTDVKGEEGLIELEHELPDELPDGITKLTYYIKVLRGTESVTQTFTVEEYVLPRFEVEVITPPYILITDPTCTVKVCGTYTYGKPVQGSVQANITADGGRYDYRTRTRSSREVLPFDIPSTDADGCAVINVNLTRLELTSRKFYIYGSLQVSARFTETATGETLTDKSTSRMSSSTLTMRAINKNNFFKPALPYFGAVKVTNPDGAPKPGESIIVRCNALASPVEATSNDQGFAVFTLPLQGIYEGFDSGNSLSCNVTAKDFQENIPYDPDVRTLNRPITYFSLSPMYSPTDNYLEVEAVIQFGNPIQVGGSVFISVHMTKDCTGGPRILAVSRGNILQDFEYEASRQENANGIIYDVTFVLTPLMTPFLDLLVYGILDDGEMIADSLEIESNAVFENEVHIQVAEGQDATLEPGARTSIEIIASPGSLCAVGVVDKSVHILGGNNQMKTSDVVSFLNTFQLRSGDNDNPRCQDHEDEPIKIDDGLVWFGFSPYRRDPYPLYSDVTKEFEQLGLVRISNLKVETSPCQTPEPYPYYARGQVLERADSEPDFAQAFSVSAASAGPAIRKYFPETWLWSMQRPEESPTVTVPDTITEWVGSGFCISSTTGFGISKPFSVTAFKPFFADYNLPYSVIRGEELELQINIFNYLEKCLPVEVTLTPSAKFEIKEKSNSKYTCVCSSSEPTQVTFNVRPTALGDVDLETTVIVVYDQTVCGEKPVETDVTHSDGLSKPILVEPEGEEVEESLSLYFCPKEEKGGTFRHTFEISVPVEYVTDSGRVTIFLSGDILGNSLANIDRLLRIPSGCGEQNMIGFVPNIYALQYLQATEQADQSQVNKALSNMRKGYQKQLNYRRSLGSYSAFGNRDPSGSAWLTAYVIRSFAQASPFITIDTKDLDVSINWLYNTLGQEENGCFRSRGRVIHKEMQGGVNDEVTLTAFIVVSLLEAQQPLETQAIQDALSCIRNSIGSLEDTYTAALIAYALSIAKDPNVNKVLEYLESVAKNENGVRYWVSSRTQTPGSRRYNRGATASSLSIEMTAYVLLTYINVYGHDAIARGSAISKWIVSQQGPNGGFKSSQDTVVAIHALAEYARLVFGGESNLGIAFSYQEPFSLNSIFIIHDGNRLVLQRADLDQLPKAIDVIAIGEGCALLQVIIRYNVHPALPPAPAFDISFNVGPTTQQLEDACQESFNLNVCVRYLGDDGFSNMAMVEVKLVTGFVPEQKSFDLLKNGFYPELTRAEQRDKKVVLYFNEFNAKPLCFDLEVRRVHHVTDPKPGLITVYDYYAPDVILSTEWHMECVIEAKGVPLQLPAVEEPEELPPGDQL

Target ORF information:

RefSeq Version	XM_011677837.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus alpha-2-macroglobulin-like protein 1 (LOC100893314), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011677837.1

ORF Insert Sequence:

ATGAGGCTTA TCGTAAGTCT TCTCATCTTC ACTATCGCAG TGATGGTGAG AGGACAGGAA 
GATGGAGATG AACTGCGGGT GGCTCTCACT TTACCTCCAA TTGCCGAAGA AGTTACACAA 
CTACCCGAAC CGACTACAAC TCCAGCACTA CCACTAAGCC CTGAGGAACT CCGAGAAGAG 
GATTTCAACA AGGAAATCTC CTACCTCTTC CTAATCCCTA AACTCTTCTA CGTGCAAACC 
AAAGAGAGGA TATGTCTCCT GGTAACCAAG AGCGACGGTC TCCTGGACTA CCGGGTGTCT 
CTCCAGAAGA TAAATGCAGG TCAATACGAA GAATACTGGA GTCCTGGAGA ACAGGAGGCT 
AAACAAGGAG AGAACTGTTT CGAATTTGAT GTACCAACGA TCGAGTCGGG ATCCAATCAA 
AGACAGAAAG TTCGTCTTCA ACTGCGACGA AAGGGAGCCG AAGACTTGAA CGAGGACGGA 
TATGAAGTCA CGAAAGAGAG ATTCGTGAGG ATCCAGGCCC AAGGCGGGTC GAAAACATAC 
GTCCAGACTG ACAAACCCAT TTACCAACCC GGTCAGACAG TGAGATTCCG TGCTCTGACA 
TTGAACAGTG AACTGAAAAC CGTCACAGAA CCATTCGATT CTGTTATAGT GGAGAGTCAG 
TCTCGCCAGC GCGTCAACCA GTGGACGGAC GTCAAGGGTG AGGAAGGGCT GATTGAACTC 
GAGCACGAAC TCCCGGACGA GCTCCCGGAC GGGATCACGA AACTGACTTA CTACATCAAG 
GTCTTGCGAG GCACCGAATC AGTAACGCAG ACATTTACTG TTGAAGAGTA CGTTCTTCCA 
AGGTTTGAGG TTGAAGTTAT AACACCTCCG TACATTCTCA TCACCGATCC TACTTGCACA 
GTCAAGGTTT GTGGAACTTA CACGTATGGT AAACCCGTTC AAGGATCTGT GCAAGCAAAC 
ATCACTGCTG ACGGGGGGCG ATACGACTAC AGGACTAGAA CTCGGTCTTC TAGAGAGGTT 
CTACCGTTCG ACATACCGAG TACTGATGCC GATGGTTGTG CTGTTATCAA TGTTAATCTC 
ACACGCTTAG AGCTCACATC GAGAAAGTTC TATATCTATG GAAGTCTGCA AGTATCCGCC 
AGGTTTACCG AAACGGCTAC AGGTGAAACG CTGACTGATA AATCTACGTC GCGTATGAGC 
TCATCTACAC TCACCATGAG GGCTATAAAC AAAAACAATT TCTTCAAGCC TGCACTACCA 
TACTTTGGAG CGGTCAAGGT GACTAATCCC GATGGAGCCC CAAAGCCTGG TGAGAGCATT 
ATCGTGCGAT GCAATGCTTT GGCTTCACCT GTAGAAGCCA CTTCCAATGA CCAAGGATTC 
GCTGTATTCA CTTTACCACT TCAGGGAATC TACGAAGGAT TCGATAGTGG AAACTCTCTC 
AGCTGCAATG TGACAGCCAA AGACTTCCAA GAGAACATTC CATATGACCC CGATGTCCGG 
ACCCTGAATC GACCAATCAC TTACTTCAGT CTGAGTCCTA TGTACTCCCC GACCGATAAC 
TATCTGGAGG TCGAAGCGGT GATCCAATTT GGCAATCCAA TACAAGTTGG CGGCAGCGTA 
TTTATTTCAG TCCACATGAC CAAGGATTGC ACTGGTGGAC CCAGGATACT GGCTGTGTCT 
CGGGGGAACA TCCTCCAGGA TTTCGAATAC GAGGCATCCC GTCAGGAGAA CGCAAACGGC 
ATCATTTATG ACGTCACCTT TGTTCTCACG CCGTTAATGA CCCCGTTTCT TGACCTCCTG 
GTCTATGGTA TCCTGGATGA CGGAGAGATG ATCGCCGATA GTCTCGAAAT CGAAAGCAAC 
GCCGTCTTTG AGAATGAGGT TCACATCCAG GTCGCCGAAG GACAGGATGC TACACTCGAG 
CCGGGGGCAC GGACGAGCAT CGAGATCATA GCATCCCCCG GATCGTTGTG CGCTGTCGGA 
GTGGTCGATA AGAGCGTGCA TATTCTAGGA GGCAACAACC AGATGAAGAC CAGCGACGTG 
GTCAGTTTTC TAAACACATT CCAGTTGAGG TCAGGTGACA ATGATAATCC GCGGTGCCAG 
GATCACGAGG ACGAACCAAT CAAGATTGAT GATGGCTTGG TTTGGTTTGG TTTCTCTCCG 
TATCGTAGAG ATCCATATCC GTTATACTCG GACGTGACCA AGGAATTTGA GCAACTGGGT 
CTTGTTCGGA TTTCTAATCT TAAAGTCGAG ACGAGTCCAT GTCAGACGCC AGAACCCTAT 
CCATATTACG CTCGGGGTCA GGTGCTTGAG AGAGCTGATT CTGAGCCAGA CTTTGCACAA 
GCATTTTCAG TTTCGGCGGC TTCAGCAGGT CCAGCCATTC GCAAATATTT TCCAGAGACT 
TGGCTCTGGT CTATGCAACG CCCAGAGGAA TCCCCAACCG TCACCGTCCC TGATACCATT 
ACAGAGTGGG TTGGAAGTGG GTTCTGTATC TCCTCAACGA CTGGCTTTGG TATCTCCAAA 
CCCTTCTCGG TGACAGCTTT CAAACCGTTC TTTGCGGATT ACAATCTCCC TTACTCTGTC 
ATACGAGGCG AAGAGCTGGA GCTTCAAATT AACATCTTCA ACTACTTGGA AAAGTGCTTA 
CCGGTTGAGG TAACTCTGAC TCCGTCTGCT AAATTTGAGA TCAAAGAGAA ATCCAACAGC 
AAATACACTT GCGTATGCAG CAGCTCTGAA CCAACCCAAG TGACCTTTAA CGTTCGGCCC 
ACAGCATTAG GGGACGTTGA TCTGGAAACA ACTGTGATCG TTGTGTATGA CCAGACTGTA 
TGCGGAGAGA AACCTGTTGA AACTGATGTA ACACACAGTG ATGGACTAAG CAAACCTATT 
CTCGTAGAGC CTGAAGGAGA GGAGGTGGAG GAATCGCTTA GTTTGTACTT CTGTCCAAAA 
GAAGAGAAAG GCGGCACATT CAGGCATACA TTTGAGATCT CAGTTCCGGT GGAGTATGTT 
ACGGACTCGG GCAGAGTTAC TATATTCTTG TCAGGGGACA TTCTCGGCAA TAGTCTTGCG 
AACATCGACA GACTGCTCAG GATCCCGTCT GGATGCGGTG AGCAGAACAT GATCGGGTTT 
GTACCAAACA TCTACGCGCT CCAGTACCTC CAAGCGACCG AACAAGCTGA CCAATCACAA 
GTCAACAAAG CCTTGTCAAA TATGAGGAAA GGATACCAGA AGCAGCTCAA TTACCGGCGT 
AGCCTCGGGT CTTACTCAGC GTTTGGTAAC CGGGACCCAT CAGGGAGTGC ATGGCTGACA 
GCCTATGTGA TTCGCTCCTT CGCTCAGGCC AGTCCATTCA TTACCATCGA TACAAAGGAC 
CTTGACGTCA GTATTAATTG GCTGTACAAC ACCCTCGGAC AAGAGGAGAA TGGGTGTTTC 
CGGTCAAGGG GTCGCGTAAT CCACAAAGAG ATGCAGGGTG GAGTGAATGA TGAGGTGACT 
TTGACAGCGT TCATAGTCGT TAGCTTACTG GAAGCCCAGC AGCCTTTGGA AACCCAGGCT 
ATCCAAGACG CGTTGTCCTG TATCCGCAAC TCTATCGGCA GTTTGGAAGA CACATACACT 
GCCGCCCTCA TCGCCTACGC CCTGAGTATA GCTAAGGATC CCAACGTCAA TAAAGTTCTG 
GAATACCTCG AATCAGTCGC CAAAAATGAA AATGGAGTGA GATACTGGGT AAGCAGCCGA 
ACCCAGACTC CGGGATCGCG TAGATACAAC CGTGGTGCAA CCGCTTCCTC CCTCAGCATT 
GAGATGACCG CGTACGTACT CCTAACCTAC ATCAATGTTT ACGGTCATGA CGCCATAGCC 
CGCGGCTCGG CTATCTCCAA GTGGATTGTC TCACAGCAAG GCCCTAACGG AGGGTTTAAA 
TCGTCTCAGG ACACCGTCGT CGCCATTCAC GCTCTCGCTG AGTATGCCCG TCTTGTGTTC 
GGCGGCGAGT CTAACCTGGG TATTGCGTTT AGCTACCAGG AGCCTTTTTC GTTGAACTCA 
ATATTCATCA TCCATGATGG AAATCGTCTC GTTCTCCAGC GGGCCGACCT CGACCAACTT 
CCAAAGGCCA TCGACGTCAT CGCTATTGGC GAAGGATGTG CCCTTCTTCA GGTTATCATC 
CGATATAACG TTCACCCGGC TCTTCCTCCC GCACCAGCCT TCGACATCAG TTTTAACGTG 
GGTCCAACGA CGCAACAACT TGAAGACGCC TGCCAAGAAA GCTTTAATCT CAATGTGTGT 
GTGAGGTATC TCGGTGATGA TGGATTTTCC AACATGGCCA TGGTCGAGGT GAAGCTAGTT 
ACTGGTTTCG TTCCTGAACA AAAGTCCTTT GATTTACTGA AGAATGGGTT CTACCCAGAA 
CTCACTAGGG CTGAACAAAG AGACAAGAAG GTCGTCTTGT ACTTCAACGA GTTTAACGCC 
AAACCGTTGT GCTTTGATCT GGAGGTTAGA CGTGTCCATC ACGTCACAGA TCCCAAGCCC 
GGTCTCATCA CGGTTTACGA CTACTACGCG CCAGATGTCA TTCTCTCCAC CGAGTGGCAC 
ATGGAATGTG TAATCGAAGC GAAAGGTGTA CCTCTTCAAC TACCTGCGGT CGAAGAGCCT 
GAGGAGCTAC CACCCGGCGA CCAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100893314 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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