ORF » Species Summary » Sarcophilus harrisii » CHFR cDNA ORF clone

CHFR cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

The following CHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSa122654 XM_003760919.2
Latest version!		 Sarcophilus harrisii checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OSa159194 XM_031956428.1
Latest version!		 Sarcophilus harrisii checkpoint with forkhead and ring finger domains (CHFR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OSa159194 XM_023496942.1
Latest version!		 Sarcophilus harrisii checkpoint with forkhead and ring finger domains (CHFR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CHFR ( XM_003760919.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003760967.1 Sarcophilus harrisii checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA.
    Price & Availability↑
    CloneID OSa122654
    Clone ID Related Accession (Same CDS sequence) XM_003760919.2
    Accession Version XM_003760919.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1431532800000
    Organism Sarcophilus harrisii(Tasmanian devil)
    Product E3 ubiquitin-protein ligase CHFR
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823439.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003760919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGCAGC CGTGGGGGAG GCTGATACGC CTGGGCGCCG CCGAGGGCGA GCCCCCCGTG 
    CTGCTGCGGA GGAAGGAGTG TACCATCGGG CGCAGGAAAG GTTGTGACCT TTCATTCCCT 
    GGTAACAAAT TGGTGTCTGG AGATCACTGT AAAATAGTGG TGGATGAAGA ATCTGGTCAG 
    GTCTCTCTAG AGGATACCAG CACTAATGGA ACTGTAATTA ATAAAGTGAG GATAGTGAAG 
    AAGCAGTCAT ATGCTTTACA GACTGGGGAT GTCATCTACG TGGTTTACAG GAAGAACGAG 
    CCAGAGCAAA ATGTTGCATA TCTCTATGAA TCTTTGAAAG CAAAGCCAAG CATATCACCA 
    GAACTCACTG AGGCTACTGG AAGAAATGTG TGCAACGTGA CCAAAGATAC CTCAGGTTCA 
    GAAGGAAGCA ATGATACCCA GGTTATGCCA TCCTTGCCCA ATGATCAGTC TTGCTTTGAG 
    GAACCACAGC CATCTACATC CACATCAGAC CTCTTTAATA CGTCCTCTAC CTCTCTCCTG 
    GAGTCTGCTA CTGCTGAGCG GGAGCATCCC TCTAGACCTG GTGGAGCCAG GTGTGTAAGC 
    ATATCATCAC CTGAAGATCA GTATCAATTT GCCACATGTG ATGATACTTC CAGCTTCTTA 
    TCAGCTCCTC TGGACAAAGA GGGAACCTCA GCTTTCCTAT CCAAACCTCA GGACTGGGAA 
    GACTTGGGGC CTGCTAAAAA GAAAATTAAA GGAGATCTCC AGCCGTATGT GGTGTGCCGA 
    CAGTGTCCCG GTTATCGCAG GCAGGCCGTG CCACCCTCCC CCTGTCTGCT GCCTGAGAGG 
    GACGGAGGTG CCTTCGAGGC CCCTGCAGAT GCCCCCTCCA CCTCTGCCAG CTTTCCTGCA 
    GCCACCCAGG ACTATGTGTG TCCTTCACAA GGAAGTCACG CCATATGCAC CTGCTGTTTC 
    CAGCCCATGC CAGACCGTCG AGCTGAGCGG GAGCAGAACC CGCATGTCGT TGCCCCCCAG 
    CAGTGCGCCG TGTGCTCACA GCCCTTCTGC CACCTGTATT GGGGCTGTAC CCGGACAGGC 
    TGCTCGGGCT GCCTGGCACC GTTCTGCGAG ATAAATCTTG GTGAAAAATG TTTAGATGGT 
    GTACTAAACA ACAACAACTA CGAATCAGAT ATTCTAAAGG CTTACCTAAC ATCCCGGAAC 
    TTGACATGGA AGAATGTGTT AAATGAAAGT TTGCTGGCTC TTCAGAGGGG ACTGTTTCTC 
    TTGTCAGATT ACCGAATTAC TGGAAATACT GTGCTTTGTT ACTGCTGTGG CCTACGCAAC 
    TTTCGAGAGC TGACATACCA GTACAGACAA AACATCCCTG CTTCAGAATT ACCAGGGACT 
    GTAACATCTC GTCCTGATTG CTACTGGGGG CGCAACTGCC GGACTCAGGT TAAAGCTCAC 
    CATGCCATGA AATTCAATCA TATTTGTGAA CAAACACGCT TCAAGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003760967.1
    CDS22..1512
    Translation

    Target ORF information:

    RefSeq Version XM_003760919.2
    Organism Sarcophilus harrisii(Tasmanian devil)
    Definition Sarcophilus harrisii checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (CHFR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003760919.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGCAGC CGTGGGGGAG GCTGATACGC CTGGGCGCCG CCGAGGGCGA GCCCCCCGTG 
    CTGCTGCGGA GGAAGGAGTG TACCATCGGG CGCAGGAAAG GTTGTGACCT TTCATTCCCT 
    GGTAACAAAT TGGTGTCTGG AGATCACTGT AAAATAGTGG TGGATGAAGA ATCTGGTCAG 
    GTCTCTCTAG AGGATACCAG CACTAATGGA ACTGTAATTA ATAAAGTGAG GATAGTGAAG 
    AAGCAGTCAT ATGCTTTACA GACTGGGGAT GTCATCTACG TGGTTTACAG GAAGAACGAG 
    CCAGAGCAAA ATGTTGCATA TCTCTATGAA TCTTTGAAAG CAAAGCCAAG CATATCACCA 
    GAACTCACTG AGGCTACTGG AAGAAATGTG TGCAACGTGA CCAAAGATAC CTCAGGTTCA 
    GAAGGAAGCA ATGATACCCA GGTTATGCCA TCCTTGCCCA ATGATCAGTC TTGCTTTGAG 
    GAACCACAGC CATCTACATC CACATCAGAC CTCTTTAATA CGTCCTCTAC CTCTCTCCTG 
    GAGTCTGCTA CTGCTGAGCG GGAGCATCCC TCTAGACCTG GTGGAGCCAG GTGTGTAAGC 
    ATATCATCAC CTGAAGATCA GTATCAATTT GCCACATGTG ATGATACTTC CAGCTTCTTA 
    TCAGCTCCTC TGGACAAAGA GGGAACCTCA GCTTTCCTAT CCAAACCTCA GGACTGGGAA 
    GACTTGGGGC CTGCTAAAAA GAAAATTAAA GGAGATCTCC AGCCGTATGT GGTGTGCCGA 
    CAGTGTCCCG GTTATCGCAG GCAGGCCGTG CCACCCTCCC CCTGTCTGCT GCCTGAGAGG 
    GACGGAGGTG CCTTCGAGGC CCCTGCAGAT GCCCCCTCCA CCTCTGCCAG CTTTCCTGCA 
    GCCACCCAGG ACTATGTGTG TCCTTCACAA GGAAGTCACG CCATATGCAC CTGCTGTTTC 
    CAGCCCATGC CAGACCGTCG AGCTGAGCGG GAGCAGAACC CGCATGTCGT TGCCCCCCAG 
    CAGTGCGCCG TGTGCTCACA GCCCTTCTGC CACCTGTATT GGGGCTGTAC CCGGACAGGC 
    TGCTCGGGCT GCCTGGCACC GTTCTGCGAG ATAAATCTTG GTGAAAAATG TTTAGATGGT 
    GTACTAAACA ACAACAACTA CGAATCAGAT ATTCTAAAGG CTTACCTAAC ATCCCGGAAC 
    TTGACATGGA AGAATGTGTT AAATGAAAGT TTGCTGGCTC TTCAGAGGGG ACTGTTTCTC 
    TTGTCAGATT ACCGAATTAC TGGAAATACT GTGCTTTGTT ACTGCTGTGG CCTACGCAAC 
    TTTCGAGAGC TGACATACCA GTACAGACAA AACATCCCTG CTTCAGAATT ACCAGGGACT 
    GTAACATCTC GTCCTGATTG CTACTGGGGG CGCAACTGCC GGACTCAGGT TAAAGCTCAC 
    CATGCCATGA AATTCAATCA TATTTGTGAA CAAACACGCT TCAAGAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CHFR ( XM_023496942.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352710.1 Sarcophilus harrisii checkpoint with forkhead and ring finger domains (CHFR), mRNA.
    Price & Availability↑
    CloneID OSa159194
    Clone ID Related Accession (Same CDS sequence) XM_023496942.1 , XM_031956428.1
    Accession Version XM_023496942.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1515513600000
    Organism Sarcophilus harrisii(Tasmanian devil)
    Product E3 ubiquitin-protein ligase CHFR
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823439.1) and transcript sequence (GEDN01118292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 762 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCAGC CGTGGGGGAG GCTGATACGC CTGGGCGCCG CCGAGGGCGA GCCCCCCGTG 
    CTGCTGCGGA GGAAGGAGTG TACCATCGGG CGCAGGAAAG GTTGTGACCT TTCATTCCCT 
    GGTAACAAAT TGGTGTCTGG AGATCACTGT AAAATAGTGG TGGATGAAGA ATCTGGTCAG 
    GTCTCTCTAG AGGATACCAG CACTAATGGA ACTGTAATTA ATAAAGTGAG GATAGTGAAG 
    AAGCAGTCAT ATGCTTTACA GACTGGGGAT GTCATCTACG TGGTTTACAG GAAGAACGAG 
    CCAGAGCAAA ATGTTGCATA TCTCTATGAA TCTTTGAAAG CAAAGCCAAG CATATCACCA 
    GAACTCACTG AGGCTACTGG AAGAAATGTG TGCAACGTGA CCAAAGATAC CTCAGGTTCA 
    GAAGGAAGCA ATGATACCCA GGTTATGCCA TCCTTGCCCA ATGATCAGTC TTGCTTTGAG 
    GAACCACAGC CATCTACATC CACATCAGAC CTCTTTAATA CGTCCTCTAC CTCTCTCCTG 
    GAGTCTGCTA CTGCTGAGCG GGAGCATCCC TCTAGACCTG GTGGAGCCAG GTGTGTAAGC 
    ATATCATCAC CTGAAGATCA GTATCAATTT GCCACATGTG ATGATACTTC CAGCTTCTTA 
    TCAGCTCCTC TGGACAAAGA GGGAACCTCA GCTTTCCTAT CCAAACCTCA GGACTGGGAA 
    GACTTGGGGC CTGCTAAAAA GAAAATTAAA GGAGATGGGC ATACGAATCT GAGTCTGCAG 
    TTGTCTGTTG CAGACCAGTG TAAAAACAGC AGGGCCGTTC CTGAGGAAGT GAAAGCAGAA 
    AATATGAAAC CAGACAAAAT GGAAGAAACT CTCACTTGCA TTATTTGTCA GGAGTTGCTG 
    CACGACTGTG TGAGTCTGCA GCCCTGCATG CACACCTTCT GTGCCGCTTG CTATTCAGGT 
    TGGATGGAGC GATCCTCCCT CTGTCCCACG TGCCGGTGTC CGGTCGAACG TATATGCAAA 
    AACCACATCC TGAACAACCT GGTTGAGGCC TACCTTATCC AGCACCCAGA TAAGTGCCGT 
    AATGAAGAGG ATGTGCGCAA TATGGATGCC AGGAATAAAA TCACACAGGA CATGCTTCAG 
    CCAAAAGTCA GAAGGTCCTT CTCTGATGAG GAGGGAAGTT CCGAGGACCT GTTGGAACTG 
    TCTGATGTGG ACAGCGAATC TTCAGATATC AGCCAGCCGT ATGTGGTGTG CCGACAGTGT 
    CCCGGTTATC GCAGGCAGGC CGTGCCACCC TCCCCCTGTC TGCTGCCTGA GAGGGACGGA 
    GGTGCCTTCG AGGCCCCTGC AGATGCCCCC TCCACCTCTG CCAGCTTTCC TGCAGCCACC 
    CAGGACTATG TGTGTCCTTC ACAAGGAAGT CACGCCATAT GCACCTGCTG TTTCCAGCCC 
    ATGCCAGACC GTCGAGCTGA GCGGGAGCAG AACCCGCATG TCGTTGCCCC CCAGCAGTGC 
    GCCGTGTGCT CACAGCCCTT CTGCCACCTG TATTGGGGCT GTACCCGGAC AGGCTGCTCG 
    GGCTGCCTGG CACCGTTCTG CGAGATAAAT CTTGGTGAAA AATGTTTAGA TGGTGTACTA 
    AACAACAACA ACTACGAATC AGATATTCTA AAGGCTTACC TAACATCCCG GAACTTGACA 
    TGGAAGAATG TGTTAAATGA AAGTTTGCTG GCTCTTCAGA GGGGACTGTT TCTCTTGTCA 
    GATTACCGAA TTACTGGAAA TACTGTGCTT TGTTACTGCT GTGGCCTACG CAACTTTCGA 
    GAGCTGACAT ACCAGTACAG ACAAAACATC CCTGCTTCAG AATTACCAGG GACTGTAACA 
    TCTCGTCCTG ATTGCTACTG GGGGCGCAAC TGCCGGACTC AGGTTAAAGC TCACCATGCC 
    ATGAAATTCA ATCATATTTG TGAACAAACA CGCTTCAAGA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023352710.1
    CDS1725..3689
    Translation

    Target ORF information:

    RefSeq Version XM_023496942.1
    Organism Sarcophilus harrisii(Tasmanian devil)
    Definition Sarcophilus harrisii checkpoint with forkhead and ring finger domains (CHFR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_023496942.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCAGC CGTGGGGGAG GCTGATACGC CTGGGCGCCG CCGAGGGCGA GCCCCCCGTG 
    CTGCTGCGGA GGAAGGAGTG TACCATCGGG CGCAGGAAAG GTTGTGACCT TTCATTCCCT 
    GGTAACAAAT TGGTGTCTGG AGATCACTGT AAAATAGTGG TGGATGAAGA ATCTGGTCAG 
    GTCTCTCTAG AGGATACCAG CACTAATGGA ACTGTAATTA ATAAAGTGAG GATAGTGAAG 
    AAGCAGTCAT ATGCTTTACA GACTGGGGAT GTCATCTACG TGGTTTACAG GAAGAACGAG 
    CCAGAGCAAA ATGTTGCATA TCTCTATGAA TCTTTGAAAG CAAAGCCAAG CATATCACCA 
    GAACTCACTG AGGCTACTGG AAGAAATGTG TGCAACGTGA CCAAAGATAC CTCAGGTTCA 
    GAAGGAAGCA ATGATACCCA GGTTATGCCA TCCTTGCCCA ATGATCAGTC TTGCTTTGAG 
    GAACCACAGC CATCTACATC CACATCAGAC CTCTTTAATA CGTCCTCTAC CTCTCTCCTG 
    GAGTCTGCTA CTGCTGAGCG GGAGCATCCC TCTAGACCTG GTGGAGCCAG GTGTGTAAGC 
    ATATCATCAC CTGAAGATCA GTATCAATTT GCCACATGTG ATGATACTTC CAGCTTCTTA 
    TCAGCTCCTC TGGACAAAGA GGGAACCTCA GCTTTCCTAT CCAAACCTCA GGACTGGGAA 
    GACTTGGGGC CTGCTAAAAA GAAAATTAAA GGAGATGGGC ATACGAATCT GAGTCTGCAG 
    TTGTCTGTTG CAGACCAGTG TAAAAACAGC AGGGCCGTTC CTGAGGAAGT GAAAGCAGAA 
    AATATGAAAC CAGACAAAAT GGAAGAAACT CTCACTTGCA TTATTTGTCA GGAGTTGCTG 
    CACGACTGTG TGAGTCTGCA GCCCTGCATG CACACCTTCT GTGCCGCTTG CTATTCAGGT 
    TGGATGGAGC GATCCTCCCT CTGTCCCACG TGCCGGTGTC CGGTCGAACG TATATGCAAA 
    AACCACATCC TGAACAACCT GGTTGAGGCC TACCTTATCC AGCACCCAGA TAAGTGCCGT 
    AATGAAGAGG ATGTGCGCAA TATGGATGCC AGGAATAAAA TCACACAGGA CATGCTTCAG 
    CCAAAAGTCA GAAGGTCCTT CTCTGATGAG GAGGGAAGTT CCGAGGACCT GTTGGAACTG 
    TCTGATGTGG ACAGCGAATC TTCAGATATC AGCCAGCCGT ATGTGGTGTG CCGACAGTGT 
    CCCGGTTATC GCAGGCAGGC CGTGCCACCC TCCCCCTGTC TGCTGCCTGA GAGGGACGGA 
    GGTGCCTTCG AGGCCCCTGC AGATGCCCCC TCCACCTCTG CCAGCTTTCC TGCAGCCACC 
    CAGGACTATG TGTGTCCTTC ACAAGGAAGT CACGCCATAT GCACCTGCTG TTTCCAGCCC 
    ATGCCAGACC GTCGAGCTGA GCGGGAGCAG AACCCGCATG TCGTTGCCCC CCAGCAGTGC 
    GCCGTGTGCT CACAGCCCTT CTGCCACCTG TATTGGGGCT GTACCCGGAC AGGCTGCTCG 
    GGCTGCCTGG CACCGTTCTG CGAGATAAAT CTTGGTGAAA AATGTTTAGA TGGTGTACTA 
    AACAACAACA ACTACGAATC AGATATTCTA AAGGCTTACC TAACATCCCG GAACTTGACA 
    TGGAAGAATG TGTTAAATGA AAGTTTGCTG GCTCTTCAGA GGGGACTGTT TCTCTTGTCA 
    GATTACCGAA TTACTGGAAA TACTGTGCTT TGTTACTGCT GTGGCCTACG CAACTTTCGA 
    GAGCTGACAT ACCAGTACAG ACAAAACATC CCTGCTTCAG AATTACCAGG GACTGTAACA 
    TCTCGTCCTG ATTGCTACTG GGGGCGCAAC TGCCGGACTC AGGTTAAAGC TCACCATGCC 
    ATGAAATTCA ATCATATTTG TGAACAAACA CGCTTCAAGA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CHFR ( XM_031956428.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031812288.1 Sarcophilus harrisii checkpoint with forkhead and ring finger domains (CHFR), mRNA.
    Price & Availability↑
    CloneID OSa159194
    Clone ID Related Accession (Same CDS sequence) XM_023496942.1 , XM_031956428.1
    Accession Version XM_031956428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1577635200000
    Organism Sarcophilus harrisii(Tasmanian devil)
    Product E3 ubiquitin-protein ligase CHFR
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCAGC CGTGGGGGAG GCTGATACGC CTGGGCGCCG CCGAGGGCGA GCCCCCCGTG 
    CTGCTGCGGA GGAAGGAGTG TACCATCGGG CGCAGGAAAG GTTGTGACCT TTCATTCCCT 
    GGTAACAAAT TGGTGTCTGG AGATCACTGT AAAATAGTGG TGGATGAAGA ATCTGGTCAG 
    GTCTCTCTAG AGGATACCAG CACTAATGGA ACTGTAATTA ATAAAGTGAG GATAGTGAAG 
    AAGCAGTCAT ATGCTTTACA GACTGGGGAT GTCATCTACG TGGTTTACAG GAAGAACGAG 
    CCAGAGCAAA ATGTTGCATA TCTCTATGAA TCTTTGAAAG CAAAGCCAAG CATATCACCA 
    GAACTCACTG AGGCTACTGG AAGAAATGTG TGCAACGTGA CCAAAGATAC CTCAGGTTCA 
    GAAGGAAGCA ATGATACCCA GGTTATGCCA TCCTTGCCCA ATGATCAGTC TTGCTTTGAG 
    GAACCACAGC CATCTACATC CACATCAGAC CTCTTTAATA CGTCCTCTAC CTCTCTCCTG 
    GAGTCTGCTA CTGCTGAGCG GGAGCATCCC TCTAGACCTG GTGGAGCCAG GTGTGTAAGC 
    ATATCATCAC CTGAAGATCA GTATCAATTT GCCACATGTG ATGATACTTC CAGCTTCTTA 
    TCAGCTCCTC TGGACAAAGA GGGAACCTCA GCTTTCCTAT CCAAACCTCA GGACTGGGAA 
    GACTTGGGGC CTGCTAAAAA GAAAATTAAA GGAGATGGGC ATACGAATCT GAGTCTGCAG 
    TTGTCTGTTG CAGACCAGTG TAAAAACAGC AGGGCCGTTC CTGAGGAAGT GAAAGCAGAA 
    AATATGAAAC CAGACAAAAT GGAAGAAACT CTCACTTGCA TTATTTGTCA GGAGTTGCTG 
    CACGACTGTG TGAGTCTGCA GCCCTGCATG CACACCTTCT GTGCCGCTTG CTATTCAGGT 
    TGGATGGAGC GATCCTCCCT CTGTCCCACG TGCCGGTGTC CGGTCGAACG TATATGCAAA 
    AACCACATCC TGAACAACCT GGTTGAGGCC TACCTTATCC AGCACCCAGA TAAGTGCCGT 
    AATGAAGAGG ATGTGCGCAA TATGGATGCC AGGAATAAAA TCACACAGGA CATGCTTCAG 
    CCAAAAGTCA GAAGGTCCTT CTCTGATGAG GAGGGAAGTT CCGAGGACCT GTTGGAACTG 
    TCTGATGTGG ACAGCGAATC TTCAGATATC AGCCAGCCGT ATGTGGTGTG CCGACAGTGT 
    CCCGGTTATC GCAGGCAGGC CGTGCCACCC TCCCCCTGTC TGCTGCCTGA GAGGGACGGA 
    GGTGCCTTCG AGGCCCCTGC AGATGCCCCC TCCACCTCTG CCAGCTTTCC TGCAGCCACC 
    CAGGACTATG TGTGTCCTTC ACAAGGAAGT CACGCCATAT GCACCTGCTG TTTCCAGCCC 
    ATGCCAGACC GTCGAGCTGA GCGGGAGCAG AACCCGCATG TCGTTGCCCC CCAGCAGTGC 
    GCCGTGTGCT CACAGCCCTT CTGCCACCTG TATTGGGGCT GTACCCGGAC AGGCTGCTCG 
    GGCTGCCTGG CACCGTTCTG CGAGATAAAT CTTGGTGAAA AATGTTTAGA TGGTGTACTA 
    AACAACAACA ACTACGAATC AGATATTCTA AAGGCTTACC TAACATCCCG GAACTTGACA 
    TGGAAGAATG TGTTAAATGA AAGTTTGCTG GCTCTTCAGA GGGGACTGTT TCTCTTGTCA 
    GATTACCGAA TTACTGGAAA TACTGTGCTT TGTTACTGCT GTGGCCTACG CAACTTTCGA 
    GAGCTGACAT ACCAGTACAG ACAAAACATC CCTGCTTCAG AATTACCAGG GACTGTAACA 
    TCTCGTCCTG ATTGCTACTG GGGGCGCAAC TGCCGGACTC AGGTTAAAGC TCACCATGCC 
    ATGAAATTCA ATCATATTTG TGAACAAACA CGCTTCAAGA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031812288.1
    CDS445..2409
    Translation

    Target ORF information:

    RefSeq Version XM_031956428.1
    Organism Sarcophilus harrisii(Tasmanian devil)
    Definition Sarcophilus harrisii checkpoint with forkhead and ring finger domains (CHFR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031956428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCAGC CGTGGGGGAG GCTGATACGC CTGGGCGCCG CCGAGGGCGA GCCCCCCGTG 
    CTGCTGCGGA GGAAGGAGTG TACCATCGGG CGCAGGAAAG GTTGTGACCT TTCATTCCCT 
    GGTAACAAAT TGGTGTCTGG AGATCACTGT AAAATAGTGG TGGATGAAGA ATCTGGTCAG 
    GTCTCTCTAG AGGATACCAG CACTAATGGA ACTGTAATTA ATAAAGTGAG GATAGTGAAG 
    AAGCAGTCAT ATGCTTTACA GACTGGGGAT GTCATCTACG TGGTTTACAG GAAGAACGAG 
    CCAGAGCAAA ATGTTGCATA TCTCTATGAA TCTTTGAAAG CAAAGCCAAG CATATCACCA 
    GAACTCACTG AGGCTACTGG AAGAAATGTG TGCAACGTGA CCAAAGATAC CTCAGGTTCA 
    GAAGGAAGCA ATGATACCCA GGTTATGCCA TCCTTGCCCA ATGATCAGTC TTGCTTTGAG 
    GAACCACAGC CATCTACATC CACATCAGAC CTCTTTAATA CGTCCTCTAC CTCTCTCCTG 
    GAGTCTGCTA CTGCTGAGCG GGAGCATCCC TCTAGACCTG GTGGAGCCAG GTGTGTAAGC 
    ATATCATCAC CTGAAGATCA GTATCAATTT GCCACATGTG ATGATACTTC CAGCTTCTTA 
    TCAGCTCCTC TGGACAAAGA GGGAACCTCA GCTTTCCTAT CCAAACCTCA GGACTGGGAA 
    GACTTGGGGC CTGCTAAAAA GAAAATTAAA GGAGATGGGC ATACGAATCT GAGTCTGCAG 
    TTGTCTGTTG CAGACCAGTG TAAAAACAGC AGGGCCGTTC CTGAGGAAGT GAAAGCAGAA 
    AATATGAAAC CAGACAAAAT GGAAGAAACT CTCACTTGCA TTATTTGTCA GGAGTTGCTG 
    CACGACTGTG TGAGTCTGCA GCCCTGCATG CACACCTTCT GTGCCGCTTG CTATTCAGGT 
    TGGATGGAGC GATCCTCCCT CTGTCCCACG TGCCGGTGTC CGGTCGAACG TATATGCAAA 
    AACCACATCC TGAACAACCT GGTTGAGGCC TACCTTATCC AGCACCCAGA TAAGTGCCGT 
    AATGAAGAGG ATGTGCGCAA TATGGATGCC AGGAATAAAA TCACACAGGA CATGCTTCAG 
    CCAAAAGTCA GAAGGTCCTT CTCTGATGAG GAGGGAAGTT CCGAGGACCT GTTGGAACTG 
    TCTGATGTGG ACAGCGAATC TTCAGATATC AGCCAGCCGT ATGTGGTGTG CCGACAGTGT 
    CCCGGTTATC GCAGGCAGGC CGTGCCACCC TCCCCCTGTC TGCTGCCTGA GAGGGACGGA 
    GGTGCCTTCG AGGCCCCTGC AGATGCCCCC TCCACCTCTG CCAGCTTTCC TGCAGCCACC 
    CAGGACTATG TGTGTCCTTC ACAAGGAAGT CACGCCATAT GCACCTGCTG TTTCCAGCCC 
    ATGCCAGACC GTCGAGCTGA GCGGGAGCAG AACCCGCATG TCGTTGCCCC CCAGCAGTGC 
    GCCGTGTGCT CACAGCCCTT CTGCCACCTG TATTGGGGCT GTACCCGGAC AGGCTGCTCG 
    GGCTGCCTGG CACCGTTCTG CGAGATAAAT CTTGGTGAAA AATGTTTAGA TGGTGTACTA 
    AACAACAACA ACTACGAATC AGATATTCTA AAGGCTTACC TAACATCCCG GAACTTGACA 
    TGGAAGAATG TGTTAAATGA AAGTTTGCTG GCTCTTCAGA GGGGACTGTT TCTCTTGTCA 
    GATTACCGAA TTACTGGAAA TACTGTGCTT TGTTACTGCT GTGGCCTACG CAACTTTCGA 
    GAGCTGACAT ACCAGTACAG ACAAAACATC CCTGCTTCAG AATTACCAGG GACTGTAACA 
    TCTCGTCCTG ATTGCTACTG GGGGCGCAAC TGCCGGACTC AGGTTAAAGC TCACCATGCC 
    ATGAAATTCA ATCATATTTG TGAACAAACA CGCTTCAAGA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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