FLNB cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	FLNB
Entrez Gene ID	100923582
Full Name	filamin B
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031953198.1	XP_031809058.1	filamin-B isoform X3
XM_031953219.1	XP_031809079.1	filamin-B isoform X6
XM_003762958.2	XP_003763006.1	filamin-B
XM_031953204.1	XP_031809064.1	filamin-B isoform X4
XM_031953212.1	XP_031809072.1	filamin-B isoform X5
XM_031953191.1	XP_031809051.1	filamin-B isoform X2
XM_031953181.1	XP_031809041.1	filamin-B isoform X1

Pathways from BioSystems
Gene Ontology

The following FLNB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLNB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa218998	XM_031953181.1 Latest version!	Sarcophilus harrisii filamin B (FLNB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa218999	XM_031953191.1 Latest version!	Sarcophilus harrisii filamin B (FLNB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa219000	XM_031953198.1 Latest version!	Sarcophilus harrisii filamin B (FLNB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa219001	XM_031953204.1 Latest version!	Sarcophilus harrisii filamin B (FLNB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa219002	XM_031953212.1 Latest version!	Sarcophilus harrisii filamin B (FLNB), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa219003	XM_031953219.1 Latest version!	Sarcophilus harrisii filamin B (FLNB), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FLNB ( XM_031953181.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809041.1 Sarcophilus harrisii filamin B (FLNB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa218998
Clone ID Related Accession (Same CDS sequence)	XM_031953181.1
Accession Version	XM_031953181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	filamin-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG CGGACGAGGT GGACTCCCAG GCCCGGCGCA GCCCATTGAA GGCCTTGTCA 
GATTTCTTTA GAGATGACCA GCAGGGTGAC TTTAATGAGA CAGGCCTGGT GGAACCGGTG 
ACTGTGACGG ACAACGGCGA TGGCACTCAC ACCGTCACCT ACACCCCCTC GCAGGAAGGC 
CCGTACATGC TGTCTGTCAA GTACGCCGAT GAGGACGTCC CCCACAGTCC CTTTAAGGTC 
AAGGTCCTCC CCACGTATGA CGCTAGCAAA GTGACCGCAA GCGGCCCCGG TCTCAGCTCC 
TATGGGGTCC CGGCCAGCTT GCCCGTGGAG TTTGCAATCG ATGCCAAAAA AGCAGGAATT 
GGGCCCCTCG CTGTCCAGAT AACGGACCAA GAAGGAAAGC CCAAAAAGGC CGACGTCCAC 
GACAACAAAG ATGGCACCTA CTCCGTGACC TACCTCCCAG ACAAGACGGG CCGCTATCTG 
ATCGGGGTCA CGTACGGCGG GGAAAACATC CCGTCTTCTC CTTACCGCAT CCGTGCAACG 
CCGACTGGAG ACGCCAGCAA GTGCATGGCC ACAGGTCCTG GGATCGCACC CACTGTCAAA 
ACTGGAGAGG AAGTTGGCTT TGTGGTGGAT GCTAAGACTG CGGGGAAAGG CAAGGTGACC 
TGCACGATCC TGACCCCGGA CGGCACGGAA GTGGAAGCCG AGGTCATCGA GAACGAGGAT 
GGGACCTACG ACATCTTCTA CACGGCAGCC AAACCCGGGA CCTACGTCAT CTATGTGCGC 
TTTGGGGGCG TGGATATCCC CAACAGCCCC TTCACCGTCA TGGCCACAGA GGAGGACGTT 
GCCGTAATGA AGGAGGCGCC GGTAAATGCA TGTCCCCCCG GCTACAGTCC TTGGGTGACG 
GAAGAAGCCT ACGTTCCCGT CAGTGACATG AATGGCCTGG GATTTAAGCC CTTTGACCTG 
GTCATTCCCT TTGCTGTACG GAAGGGAGAA GTAACCGGGG AAGTGCACAT GCCCTCCGGG 
AAGACGGCTG CCCCCGAGAT CGTCGACAAC AAAGATGGCA CCGTCACGGT TCGCTACGCG 
CCCACCGAGG TCGGCCTGCA CGAGATGCAC ATCAAGTACA TGGGGAACCA CATCCCCGAG 
AGCCCGCTCC AGTTCTATGT GAACTACCCA AATAGCGGCA GCGTGTCGGC GTACGGGCCC 
GGCCTGGTGT ACGGCGTCGC CAACAAGACG GCCACCTTCA CCATCGTCAC AGAGGATGCG 
GGAGAGGGGG GCCTGGACCT GGCCATCGAG GGGCCTTCCA AAGCTGAAAT CAGCTGCATC 
GACAACAAGG ACGGCACCTG CACGGTGACC TACCTGCCCA CACTCCCCGG GGACTACAGC 
ATCCTGGTGA AGTACAATGA CAAGCACATA CCCGGCAGCC CCTTCACCGC CAAGATCACA 
GATAACAACA GGAGGTGTTC CCAAGTGAAG CTGGGCTCGG CTGCCGACTT CCTGCTGGAC 
ATCAGCGAGA CTGACCTGAG CACACTGACC GCCAGCATCA AAGCTCCGTC CGGGCGAGAT 
GAGCCTTGCC TCTTGAAGAG GCTCCCAAAC AACCACATTG GCATTTCTTT CATCCCCCGG 
GAAGTCGGGG AACACCTGGT CAGCATTAAG AAAAATGGCA GCCACGTGGC CAACAGCCCG 
GTATCCATCA TGGTGGTTCA GTCCGAGATC GGAGACGCCC GGAGGGCCAA GGTCTCAGGC 
CGGGGCTTGA GTGAAGGGCG CACTTTTGAA ATGTCCGACT TCATTGTTGA CACAAGGGAT 
GCAGGTTACG GTGGCATATC CCTGGCGGTG GAAGGCCCCA GCAAGGTGGA CATCCAGACG 
GAAGACCTTG AAGATGGCAC CTGTAAAGTG TCCTACTTCC CCACGGTGCC GGGGGTGTAT 
ATTGTATCCA CCAAGTTTGC AGATGAACAC GTGCCAGGGA GTCCCTTCAC TGTGAAAATC 
AGCGGAGAAG GCAGGGTCAA GGAAAGCATC ACGCGCCCAA GTCGGGCACC GTCGGTGGCG 
ACCGTTGGGA GCATCTGTGA CCTCAACCTG AAAATCCCAG AAATAGACAG TGCAGACATG 
TCGGCCCACG TCACCAGCCC CTCTGGCCGC ACGACGGAGG CCGAGATCGT GCCCGTAGAC 
AAGAGCTCCC ACTGTGTCCG GTTCGTGCCC CAGGAGATGG GTGTGCACAC GGTGAGCGTC 
AAGTACCGAG GGCAGCACGT GACCGGAAGC CCGTTCCAGT TCACAGTTGG GCCCCTCGGG 
GAAGGGGGTG CCCAGAAAGT CCGCGCCGGG GGCCCCGGCC TCGAGAGAGG AGAAGCGGGA 
GTGCCAGCTG AATTTAGCAT TTGGACCAGA GAAGCAGGGG CCGGGGGTCT CTCGATTGCT 
GTCGAAGGAC CGAGTAAGGC AGAAATTGCC TTTGACGACC ACAAAAACGG GTCGTGTGGC 
GTGTCTTACA TTGCTCAAGA ACCGGGTAAC TATGAGGTGT CGATCAAGTT CAACGACGAG 
CACATCCCAG AGAGCCCCTA CTTGGTGCCC ATCATCGCTC CTTCCGACGA CGCCCGCAGA 
CTCACTGTTA TGAGCCTTCA GGAATCTGGA TTAAAAGTTA ACCAGCCAGC ATCCTTTGCG 
ATAAGGCTGA ATGGGGCCAA AGGCAAGATC GATGCAAAAG TCCACAGCCC CTCGGGTGCC 
GTCGAGGAGT GCCACGTGTC CGAGCTGGAG CCAGATAAGT ACGCCGTGCG CTTCATTCCC 
CACGAGAACG GCATTCACAC GATCGACGTC AAGTTCAATG GCAGCCACGT GGTTGGAAGC 
CCTTTTAAAG TTCGAGTCGG GGATCCAGGA CAAGCGGGAA ATCCAGCTCT CGTCTCTGCC 
TATGGAGCTG GCCTGGAAGG GGGGACCACA GGCGTCCAAT CGGAGTTTTT CATCAATACC 
ACCAGAGCTG GCCCCGGGAC ACTGTCTGTT ACAATTGAGG GACCGTCCAA GGTCAAAATG 
GACTGCCAGG AGACTCCAGA AGGTTACAAA GTCATGTATA CACCGATGGC TCCTGGGAAT 
TATCTGATCG GTGTGAAATA CGGCGGACCC AACCACATCG TTGGCAGCCC TTTCAAGGCC 
AAGGTGACAG GTCAACGCCT CGTCAGCCCG TGCTCTGCCA ACGAGACATC TTCCATCCTG 
GTTGAGTCGG TGACCAGATC CTCCACAGAA ACCTGCTACA GCGCCGTCCC CAAAGCTTCT 
TCAGACGCCA GCAAAGTGAC TTCAAAGGGA GCAGGGCTCG TGAAAGCTTT CGTGGGCCAG 
AAGAGCAGCT TCCTGGTCGA CTGCAGCAAG GCGGGTTCCA ATATGTTACT GATTGGTGTC 
CATGGTCCCA CCACGCCTTG TGAAGAAGTG TCCATGAAAC ATGTAGGAAA CCAGCAGTAT 
AATGTGACTT ATGTTGTCAA GGAGAAGGGG GATTATGTTT TGGCTGTCAA GTGGGGTGAG 
GAGCACATTC CGGGAAGTCC CTTCCATATC ACAGTACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809041.1
CDS	151..8052
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLELESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPSKPVDNAREAMQQADDWLGVPQVITPEEIIHPNVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVAGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEATGNIANKATFFDIYTAGAGVGDIEVEVEDPQGKKTVEMVIEDKGNQVYRCAYKPIQAGPHVVKVSFAGESIPKSPFTVNVGEACNPNACRASGRGLQPKGVRIRETADFKVDTKSAGSGELSVTVRGPKGLEELVKQKGFANGVYAFEYYPGTPGKYVVAITWGGHNIPKSPFEVQVGPEAGVQKVRAWGPGLHGGIAGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPYMAYIQPASGDFNPDLVQAYGPGLEKTGCIVNNLAEFTVDPKDAGKAPLKIYAQDGEGSPIEIQMKGKMDGTYACFYTPLKPIKHTISVVWGGVNIPKSPYRVGVGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCTEAGEGDVSVGIKCDAGVLGPDEEDVDFDIIHNANDTFTVKYAPPAPGRYTIKVLFASKEIPASPFRVKVDPSHDASKVRAEGPGLSKAGVENGKPTHFTVFTKGAGKAPLDVQFSGPQAGDVVHDLDIIDNYDYSHTVKYTPVQQGSTQVLVTYGGDPIPRSPFVVEVAAPLDLSKIKLNGLENRVEVGKDQEFVVDTKGAGGQGKLDVTILSPARQVVPCLVASVPGKDCSTAKYIPKEEGLYAVDVSYDGNPIPGSPFTVEASLPPDPTKVKAHGPGLEAGFVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDASKVVASGPGLEHGKVGEAGVLTVDCSEAGRGVLGMEAVSDDGVRADVHIQNNKDGTYAVTYVPLAAGMYTLSLNYGGEQVPGFPARVKVEPAVDTSKIKVYGPGIEGKDVFREATTSFTVDSRPLTKVGGEHVKASIANPSGAFTDYLLKDNADGTYEVEFTPFEKGFHTVEVTYDDVPVPNSPFQVAVTEGCHPSRVQAQGPGLKEGFTHKPNPFTVVTRGAGIGGLGITVEGPSESKISCKDNKDGSCSAEYIPFVPGDYDVNITYGNEHVPGSPFRIPVKDVIDPSKVKLAGPGLGAGVRAKVPQSFTVDTSQAGLAPLEVLVLGPRADEVDSQARRSPLKALSDFFRDDQQGDFNETGLVEPVTVTDNGDGTHTVTYTPSQEGPYMLSVKYADEDVPHSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVEFAIDAKKAGIGPLAVQITDQEGKPKKADVHDNKDGTYSVTYLPDKTGRYLIGVTYGGENIPSSPYRIRATPTGDASKCMATGPGIAPTVKTGEEVGFVVDAKTAGKGKVTCTILTPDGTEVEAEVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATEEDVAVMKEAPVNACPPGYSPWVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEVTGEVHMPSGKTAAPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGNHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDNNRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGSHVANSPVSIMVVQSEIGDARRAKVSGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRPSRAPSVATVGSICDLNLKIPEIDSADMSAHVTSPSGRTTEAEIVPVDKSSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAQKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYLVPIIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGDPGQAGNPALVSAYGAGLEGGTTGVQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPCSANETSSILVESVTRSSTETCYSAVPKASSDASKVTSKGAGLVKAFVGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKEKGDYVLAVKWGEEHIPGSPFHITVP

Target ORF information:

RefSeq Version	XM_031953181.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii filamin B (FLNB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953181.1

ORF Insert Sequence:

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG CGGACGAGGT GGACTCCCAG GCCCGGCGCA GCCCATTGAA GGCCTTGTCA 
GATTTCTTTA GAGATGACCA GCAGGGTGAC TTTAATGAGA CAGGCCTGGT GGAACCGGTG 
ACTGTGACGG ACAACGGCGA TGGCACTCAC ACCGTCACCT ACACCCCCTC GCAGGAAGGC 
CCGTACATGC TGTCTGTCAA GTACGCCGAT GAGGACGTCC CCCACAGTCC CTTTAAGGTC 
AAGGTCCTCC CCACGTATGA CGCTAGCAAA GTGACCGCAA GCGGCCCCGG TCTCAGCTCC 
TATGGGGTCC CGGCCAGCTT GCCCGTGGAG TTTGCAATCG ATGCCAAAAA AGCAGGAATT 
GGGCCCCTCG CTGTCCAGAT AACGGACCAA GAAGGAAAGC CCAAAAAGGC CGACGTCCAC 
GACAACAAAG ATGGCACCTA CTCCGTGACC TACCTCCCAG ACAAGACGGG CCGCTATCTG 
ATCGGGGTCA CGTACGGCGG GGAAAACATC CCGTCTTCTC CTTACCGCAT CCGTGCAACG 
CCGACTGGAG ACGCCAGCAA GTGCATGGCC ACAGGTCCTG GGATCGCACC CACTGTCAAA 
ACTGGAGAGG AAGTTGGCTT TGTGGTGGAT GCTAAGACTG CGGGGAAAGG CAAGGTGACC 
TGCACGATCC TGACCCCGGA CGGCACGGAA GTGGAAGCCG AGGTCATCGA GAACGAGGAT 
GGGACCTACG ACATCTTCTA CACGGCAGCC AAACCCGGGA CCTACGTCAT CTATGTGCGC 
TTTGGGGGCG TGGATATCCC CAACAGCCCC TTCACCGTCA TGGCCACAGA GGAGGACGTT 
GCCGTAATGA AGGAGGCGCC GGTAAATGCA TGTCCCCCCG GCTACAGTCC TTGGGTGACG 
GAAGAAGCCT ACGTTCCCGT CAGTGACATG AATGGCCTGG GATTTAAGCC CTTTGACCTG 
GTCATTCCCT TTGCTGTACG GAAGGGAGAA GTAACCGGGG AAGTGCACAT GCCCTCCGGG 
AAGACGGCTG CCCCCGAGAT CGTCGACAAC AAAGATGGCA CCGTCACGGT TCGCTACGCG 
CCCACCGAGG TCGGCCTGCA CGAGATGCAC ATCAAGTACA TGGGGAACCA CATCCCCGAG 
AGCCCGCTCC AGTTCTATGT GAACTACCCA AATAGCGGCA GCGTGTCGGC GTACGGGCCC 
GGCCTGGTGT ACGGCGTCGC CAACAAGACG GCCACCTTCA CCATCGTCAC AGAGGATGCG 
GGAGAGGGGG GCCTGGACCT GGCCATCGAG GGGCCTTCCA AAGCTGAAAT CAGCTGCATC 
GACAACAAGG ACGGCACCTG CACGGTGACC TACCTGCCCA CACTCCCCGG GGACTACAGC 
ATCCTGGTGA AGTACAATGA CAAGCACATA CCCGGCAGCC CCTTCACCGC CAAGATCACA 
GATAACAACA GGAGGTGTTC CCAAGTGAAG CTGGGCTCGG CTGCCGACTT CCTGCTGGAC 
ATCAGCGAGA CTGACCTGAG CACACTGACC GCCAGCATCA AAGCTCCGTC CGGGCGAGAT 
GAGCCTTGCC TCTTGAAGAG GCTCCCAAAC AACCACATTG GCATTTCTTT CATCCCCCGG 
GAAGTCGGGG AACACCTGGT CAGCATTAAG AAAAATGGCA GCCACGTGGC CAACAGCCCG 
GTATCCATCA TGGTGGTTCA GTCCGAGATC GGAGACGCCC GGAGGGCCAA GGTCTCAGGC 
CGGGGCTTGA GTGAAGGGCG CACTTTTGAA ATGTCCGACT TCATTGTTGA CACAAGGGAT 
GCAGGTTACG GTGGCATATC CCTGGCGGTG GAAGGCCCCA GCAAGGTGGA CATCCAGACG 
GAAGACCTTG AAGATGGCAC CTGTAAAGTG TCCTACTTCC CCACGGTGCC GGGGGTGTAT 
ATTGTATCCA CCAAGTTTGC AGATGAACAC GTGCCAGGGA GTCCCTTCAC TGTGAAAATC 
AGCGGAGAAG GCAGGGTCAA GGAAAGCATC ACGCGCCCAA GTCGGGCACC GTCGGTGGCG 
ACCGTTGGGA GCATCTGTGA CCTCAACCTG AAAATCCCAG AAATAGACAG TGCAGACATG 
TCGGCCCACG TCACCAGCCC CTCTGGCCGC ACGACGGAGG CCGAGATCGT GCCCGTAGAC 
AAGAGCTCCC ACTGTGTCCG GTTCGTGCCC CAGGAGATGG GTGTGCACAC GGTGAGCGTC 
AAGTACCGAG GGCAGCACGT GACCGGAAGC CCGTTCCAGT TCACAGTTGG GCCCCTCGGG 
GAAGGGGGTG CCCAGAAAGT CCGCGCCGGG GGCCCCGGCC TCGAGAGAGG AGAAGCGGGA 
GTGCCAGCTG AATTTAGCAT TTGGACCAGA GAAGCAGGGG CCGGGGGTCT CTCGATTGCT 
GTCGAAGGAC CGAGTAAGGC AGAAATTGCC TTTGACGACC ACAAAAACGG GTCGTGTGGC 
GTGTCTTACA TTGCTCAAGA ACCGGGTAAC TATGAGGTGT CGATCAAGTT CAACGACGAG 
CACATCCCAG AGAGCCCCTA CTTGGTGCCC ATCATCGCTC CTTCCGACGA CGCCCGCAGA 
CTCACTGTTA TGAGCCTTCA GGAATCTGGA TTAAAAGTTA ACCAGCCAGC ATCCTTTGCG 
ATAAGGCTGA ATGGGGCCAA AGGCAAGATC GATGCAAAAG TCCACAGCCC CTCGGGTGCC 
GTCGAGGAGT GCCACGTGTC CGAGCTGGAG CCAGATAAGT ACGCCGTGCG CTTCATTCCC 
CACGAGAACG GCATTCACAC GATCGACGTC AAGTTCAATG GCAGCCACGT GGTTGGAAGC 
CCTTTTAAAG TTCGAGTCGG GGATCCAGGA CAAGCGGGAA ATCCAGCTCT CGTCTCTGCC 
TATGGAGCTG GCCTGGAAGG GGGGACCACA GGCGTCCAAT CGGAGTTTTT CATCAATACC 
ACCAGAGCTG GCCCCGGGAC ACTGTCTGTT ACAATTGAGG GACCGTCCAA GGTCAAAATG 
GACTGCCAGG AGACTCCAGA AGGTTACAAA GTCATGTATA CACCGATGGC TCCTGGGAAT 
TATCTGATCG GTGTGAAATA CGGCGGACCC AACCACATCG TTGGCAGCCC TTTCAAGGCC 
AAGGTGACAG GTCAACGCCT CGTCAGCCCG TGCTCTGCCA ACGAGACATC TTCCATCCTG 
GTTGAGTCGG TGACCAGATC CTCCACAGAA ACCTGCTACA GCGCCGTCCC CAAAGCTTCT 
TCAGACGCCA GCAAAGTGAC TTCAAAGGGA GCAGGGCTCG TGAAAGCTTT CGTGGGCCAG 
AAGAGCAGCT TCCTGGTCGA CTGCAGCAAG GCGGGTTCCA ATATGTTACT GATTGGTGTC 
CATGGTCCCA CCACGCCTTG TGAAGAAGTG TCCATGAAAC ATGTAGGAAA CCAGCAGTAT 
AATGTGACTT ATGTTGTCAA GGAGAAGGGG GATTATGTTT TGGCTGTCAA GTGGGGTGAG 
GAGCACATTC CGGGAAGTCC CTTCCATATC ACAGTACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_031953191.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809051.1 Sarcophilus harrisii filamin B (FLNB), transcript variant X2, mRNA.

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CloneID	OSa218999
Clone ID Related Accession (Same CDS sequence)	XM_031953191.1
Accession Version	XM_031953191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	filamin-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG CGGACGAGGT GGACTCCCAG GCCCGGCGCA GCCCATTGAA GGCCTTGTCA 
GATTTCTTTA GAGATGACCA GCAGGGTGAC TTTAATGAGA CAGGCCTGGT GGAACCGGTG 
ACTGTGACGG ACAACGGCGA TGGCACTCAC ACCGTCACCT ACACCCCCTC GCAGGAAGGC 
CCGTACATGC TGTCTGTCAA GTACGCCGAT GAGGACGTCC CCCACAGTCC CTTTAAGGTC 
AAGGTCCTCC CCACGTATGA CGCTAGCAAA GTGACCGCAA GCGGCCCCGG TCTCAGCTCC 
TATGGGGTCC CGGCCAGCTT GCCCGTGGAG TTTGCAATCG ATGCCAAAAA AGCAGGAATT 
GGGCCCCTCG CTGTCCAGAT AACGGACCAA GAAGGAAAGC CCAAAAAGGC CGACGTCCAC 
GACAACAAAG ATGGCACCTA CTCCGTGACC TACCTCCCAG ACAAGACGGG CCGCTATCTG 
ATCGGGGTCA CGTACGGCGG GGAAAACATC CCGTCTTCTC CTTACCGCAT CCGTGCAACG 
CCGACTGGAG ACGCCAGCAA GTGCATGGCC ACAGGTCCTG GGATCGCACC CACTGTCAAA 
ACTGGAGAGG AAGTTGGCTT TGTGGTGGAT GCTAAGACTG CGGGGAAAGG CAAGGTGACC 
TGCACGATCC TGACCCCGGA CGGCACGGAA GTGGAAGCCG AGGTCATCGA GAACGAGGAT 
GGGACCTACG ACATCTTCTA CACGGCAGCC AAACCCGGGA CCTACGTCAT CTATGTGCGC 
TTTGGGGGCG TGGATATCCC CAACAGCCCC TTCACCGTCA TGGCCACAGA GGAGGACGTT 
GCCGTAATGA AGGAGGCGCC GGTGACGGAA GAAGCCTACG TTCCCGTCAG TGACATGAAT 
GGCCTGGGAT TTAAGCCCTT TGACCTGGTC ATTCCCTTTG CTGTACGGAA GGGAGAAGTA 
ACCGGGGAAG TGCACATGCC CTCCGGGAAG ACGGCTGCCC CCGAGATCGT CGACAACAAA 
GATGGCACCG TCACGGTTCG CTACGCGCCC ACCGAGGTCG GCCTGCACGA GATGCACATC 
AAGTACATGG GGAACCACAT CCCCGAGAGC CCGCTCCAGT TCTATGTGAA CTACCCAAAT 
AGCGGCAGCG TGTCGGCGTA CGGGCCCGGC CTGGTGTACG GCGTCGCCAA CAAGACGGCC 
ACCTTCACCA TCGTCACAGA GGATGCGGGA GAGGGGGGCC TGGACCTGGC CATCGAGGGG 
CCTTCCAAAG CTGAAATCAG CTGCATCGAC AACAAGGACG GCACCTGCAC GGTGACCTAC 
CTGCCCACAC TCCCCGGGGA CTACAGCATC CTGGTGAAGT ACAATGACAA GCACATACCC 
GGCAGCCCCT TCACCGCCAA GATCACAGAT AACAACAGGA GGTGTTCCCA AGTGAAGCTG 
GGCTCGGCTG CCGACTTCCT GCTGGACATC AGCGAGACTG ACCTGAGCAC ACTGACCGCC 
AGCATCAAAG CTCCGTCCGG GCGAGATGAG CCTTGCCTCT TGAAGAGGCT CCCAAACAAC 
CACATTGGCA TTTCTTTCAT CCCCCGGGAA GTCGGGGAAC ACCTGGTCAG CATTAAGAAA 
AATGGCAGCC ACGTGGCCAA CAGCCCGGTA TCCATCATGG TGGTTCAGTC CGAGATCGGA 
GACGCCCGGA GGGCCAAGGT CTCAGGCCGG GGCTTGAGTG AAGGGCGCAC TTTTGAAATG 
TCCGACTTCA TTGTTGACAC AAGGGATGCA GGTTACGGTG GCATATCCCT GGCGGTGGAA 
GGCCCCAGCA AGGTGGACAT CCAGACGGAA GACCTTGAAG ATGGCACCTG TAAAGTGTCC 
TACTTCCCCA CGGTGCCGGG GGTGTATATT GTATCCACCA AGTTTGCAGA TGAACACGTG 
CCAGGGAGTC CCTTCACTGT GAAAATCAGC GGAGAAGGCA GGGTCAAGGA AAGCATCACG 
CGCCCAAGTC GGGCACCGTC GGTGGCGACC GTTGGGAGCA TCTGTGACCT CAACCTGAAA 
ATCCCAGAAA TAGACAGTGC AGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCACG 
ACGGAGGCCG AGATCGTGCC CGTAGACAAG AGCTCCCACT GTGTCCGGTT CGTGCCCCAG 
GAGATGGGTG TGCACACGGT GAGCGTCAAG TACCGAGGGC AGCACGTGAC CGGAAGCCCG 
TTCCAGTTCA CAGTTGGGCC CCTCGGGGAA GGGGGTGCCC AGAAAGTCCG CGCCGGGGGC 
CCCGGCCTCG AGAGAGGAGA AGCGGGAGTG CCAGCTGAAT TTAGCATTTG GACCAGAGAA 
GCAGGGGCCG GGGGTCTCTC GATTGCTGTC GAAGGACCGA GTAAGGCAGA AATTGCCTTT 
GACGACCACA AAAACGGGTC GTGTGGCGTG TCTTACATTG CTCAAGAACC GGGTAACTAT 
GAGGTGTCGA TCAAGTTCAA CGACGAGCAC ATCCCAGAGA GCCCCTACTT GGTGCCCATC 
ATCGCTCCTT CCGACGACGC CCGCAGACTC ACTGTTATGA GCCTTCAGGA ATCTGGATTA 
AAAGTTAACC AGCCAGCATC CTTTGCGATA AGGCTGAATG GGGCCAAAGG CAAGATCGAT 
GCAAAAGTCC ACAGCCCCTC GGGTGCCGTC GAGGAGTGCC ACGTGTCCGA GCTGGAGCCA 
GATAAGTACG CCGTGCGCTT CATTCCCCAC GAGAACGGCA TTCACACGAT CGACGTCAAG 
TTCAATGGCA GCCACGTGGT TGGAAGCCCT TTTAAAGTTC GAGTCGGGGA TCCAGGACAA 
GCGGGAAATC CAGCTCTCGT CTCTGCCTAT GGAGCTGGCC TGGAAGGGGG GACCACAGGC 
GTCCAATCGG AGTTTTTCAT CAATACCACC AGAGCTGGCC CCGGGACACT GTCTGTTACA 
ATTGAGGGAC CGTCCAAGGT CAAAATGGAC TGCCAGGAGA CTCCAGAAGG TTACAAAGTC 
ATGTATACAC CGATGGCTCC TGGGAATTAT CTGATCGGTG TGAAATACGG CGGACCCAAC 
CACATCGTTG GCAGCCCTTT CAAGGCCAAG GTGACAGGTC AACGCCTCGT CAGCCCGTGC 
TCTGCCAACG AGACATCTTC CATCCTGGTT GAGTCGGTGA CCAGATCCTC CACAGAAACC 
TGCTACAGCG CCGTCCCCAA AGCTTCTTCA GACGCCAGCA AAGTGACTTC AAAGGGAGCA 
GGGCTCGTGA AAGCTTTCGT GGGCCAGAAG AGCAGCTTCC TGGTCGACTG CAGCAAGGCG 
GGTTCCAATA TGTTACTGAT TGGTGTCCAT GGTCCCACCA CGCCTTGTGA AGAAGTGTCC 
ATGAAACATG TAGGAAACCA GCAGTATAAT GTGACTTATG TTGTCAAGGA GAAGGGGGAT 
TATGTTTTGG CTGTCAAGTG GGGTGAGGAG CACATTCCGG GAAGTCCCTT CCATATCACA 
GTACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809051.1
CDS	151..8019
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLELESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPSKPVDNAREAMQQADDWLGVPQVITPEEIIHPNVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVAGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEATGNIANKATFFDIYTAGAGVGDIEVEVEDPQGKKTVEMVIEDKGNQVYRCAYKPIQAGPHVVKVSFAGESIPKSPFTVNVGEACNPNACRASGRGLQPKGVRIRETADFKVDTKSAGSGELSVTVRGPKGLEELVKQKGFANGVYAFEYYPGTPGKYVVAITWGGHNIPKSPFEVQVGPEAGVQKVRAWGPGLHGGIAGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPYMAYIQPASGDFNPDLVQAYGPGLEKTGCIVNNLAEFTVDPKDAGKAPLKIYAQDGEGSPIEIQMKGKMDGTYACFYTPLKPIKHTISVVWGGVNIPKSPYRVGVGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCTEAGEGDVSVGIKCDAGVLGPDEEDVDFDIIHNANDTFTVKYAPPAPGRYTIKVLFASKEIPASPFRVKVDPSHDASKVRAEGPGLSKAGVENGKPTHFTVFTKGAGKAPLDVQFSGPQAGDVVHDLDIIDNYDYSHTVKYTPVQQGSTQVLVTYGGDPIPRSPFVVEVAAPLDLSKIKLNGLENRVEVGKDQEFVVDTKGAGGQGKLDVTILSPARQVVPCLVASVPGKDCSTAKYIPKEEGLYAVDVSYDGNPIPGSPFTVEASLPPDPTKVKAHGPGLEAGFVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDASKVVASGPGLEHGKVGEAGVLTVDCSEAGRGVLGMEAVSDDGVRADVHIQNNKDGTYAVTYVPLAAGMYTLSLNYGGEQVPGFPARVKVEPAVDTSKIKVYGPGIEGKDVFREATTSFTVDSRPLTKVGGEHVKASIANPSGAFTDYLLKDNADGTYEVEFTPFEKGFHTVEVTYDDVPVPNSPFQVAVTEGCHPSRVQAQGPGLKEGFTHKPNPFTVVTRGAGIGGLGITVEGPSESKISCKDNKDGSCSAEYIPFVPGDYDVNITYGNEHVPGSPFRIPVKDVIDPSKVKLAGPGLGAGVRAKVPQSFTVDTSQAGLAPLEVLVLGPRADEVDSQARRSPLKALSDFFRDDQQGDFNETGLVEPVTVTDNGDGTHTVTYTPSQEGPYMLSVKYADEDVPHSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVEFAIDAKKAGIGPLAVQITDQEGKPKKADVHDNKDGTYSVTYLPDKTGRYLIGVTYGGENIPSSPYRIRATPTGDASKCMATGPGIAPTVKTGEEVGFVVDAKTAGKGKVTCTILTPDGTEVEAEVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATEEDVAVMKEAPVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEVTGEVHMPSGKTAAPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGNHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDNNRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGSHVANSPVSIMVVQSEIGDARRAKVSGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRPSRAPSVATVGSICDLNLKIPEIDSADMSAHVTSPSGRTTEAEIVPVDKSSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAQKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYLVPIIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGDPGQAGNPALVSAYGAGLEGGTTGVQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPCSANETSSILVESVTRSSTETCYSAVPKASSDASKVTSKGAGLVKAFVGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKEKGDYVLAVKWGEEHIPGSPFHITVP

Target ORF information:

RefSeq Version	XM_031953191.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii filamin B (FLNB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953191.1

ORF Insert Sequence:

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG CGGACGAGGT GGACTCCCAG GCCCGGCGCA GCCCATTGAA GGCCTTGTCA 
GATTTCTTTA GAGATGACCA GCAGGGTGAC TTTAATGAGA CAGGCCTGGT GGAACCGGTG 
ACTGTGACGG ACAACGGCGA TGGCACTCAC ACCGTCACCT ACACCCCCTC GCAGGAAGGC 
CCGTACATGC TGTCTGTCAA GTACGCCGAT GAGGACGTCC CCCACAGTCC CTTTAAGGTC 
AAGGTCCTCC CCACGTATGA CGCTAGCAAA GTGACCGCAA GCGGCCCCGG TCTCAGCTCC 
TATGGGGTCC CGGCCAGCTT GCCCGTGGAG TTTGCAATCG ATGCCAAAAA AGCAGGAATT 
GGGCCCCTCG CTGTCCAGAT AACGGACCAA GAAGGAAAGC CCAAAAAGGC CGACGTCCAC 
GACAACAAAG ATGGCACCTA CTCCGTGACC TACCTCCCAG ACAAGACGGG CCGCTATCTG 
ATCGGGGTCA CGTACGGCGG GGAAAACATC CCGTCTTCTC CTTACCGCAT CCGTGCAACG 
CCGACTGGAG ACGCCAGCAA GTGCATGGCC ACAGGTCCTG GGATCGCACC CACTGTCAAA 
ACTGGAGAGG AAGTTGGCTT TGTGGTGGAT GCTAAGACTG CGGGGAAAGG CAAGGTGACC 
TGCACGATCC TGACCCCGGA CGGCACGGAA GTGGAAGCCG AGGTCATCGA GAACGAGGAT 
GGGACCTACG ACATCTTCTA CACGGCAGCC AAACCCGGGA CCTACGTCAT CTATGTGCGC 
TTTGGGGGCG TGGATATCCC CAACAGCCCC TTCACCGTCA TGGCCACAGA GGAGGACGTT 
GCCGTAATGA AGGAGGCGCC GGTGACGGAA GAAGCCTACG TTCCCGTCAG TGACATGAAT 
GGCCTGGGAT TTAAGCCCTT TGACCTGGTC ATTCCCTTTG CTGTACGGAA GGGAGAAGTA 
ACCGGGGAAG TGCACATGCC CTCCGGGAAG ACGGCTGCCC CCGAGATCGT CGACAACAAA 
GATGGCACCG TCACGGTTCG CTACGCGCCC ACCGAGGTCG GCCTGCACGA GATGCACATC 
AAGTACATGG GGAACCACAT CCCCGAGAGC CCGCTCCAGT TCTATGTGAA CTACCCAAAT 
AGCGGCAGCG TGTCGGCGTA CGGGCCCGGC CTGGTGTACG GCGTCGCCAA CAAGACGGCC 
ACCTTCACCA TCGTCACAGA GGATGCGGGA GAGGGGGGCC TGGACCTGGC CATCGAGGGG 
CCTTCCAAAG CTGAAATCAG CTGCATCGAC AACAAGGACG GCACCTGCAC GGTGACCTAC 
CTGCCCACAC TCCCCGGGGA CTACAGCATC CTGGTGAAGT ACAATGACAA GCACATACCC 
GGCAGCCCCT TCACCGCCAA GATCACAGAT AACAACAGGA GGTGTTCCCA AGTGAAGCTG 
GGCTCGGCTG CCGACTTCCT GCTGGACATC AGCGAGACTG ACCTGAGCAC ACTGACCGCC 
AGCATCAAAG CTCCGTCCGG GCGAGATGAG CCTTGCCTCT TGAAGAGGCT CCCAAACAAC 
CACATTGGCA TTTCTTTCAT CCCCCGGGAA GTCGGGGAAC ACCTGGTCAG CATTAAGAAA 
AATGGCAGCC ACGTGGCCAA CAGCCCGGTA TCCATCATGG TGGTTCAGTC CGAGATCGGA 
GACGCCCGGA GGGCCAAGGT CTCAGGCCGG GGCTTGAGTG AAGGGCGCAC TTTTGAAATG 
TCCGACTTCA TTGTTGACAC AAGGGATGCA GGTTACGGTG GCATATCCCT GGCGGTGGAA 
GGCCCCAGCA AGGTGGACAT CCAGACGGAA GACCTTGAAG ATGGCACCTG TAAAGTGTCC 
TACTTCCCCA CGGTGCCGGG GGTGTATATT GTATCCACCA AGTTTGCAGA TGAACACGTG 
CCAGGGAGTC CCTTCACTGT GAAAATCAGC GGAGAAGGCA GGGTCAAGGA AAGCATCACG 
CGCCCAAGTC GGGCACCGTC GGTGGCGACC GTTGGGAGCA TCTGTGACCT CAACCTGAAA 
ATCCCAGAAA TAGACAGTGC AGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCACG 
ACGGAGGCCG AGATCGTGCC CGTAGACAAG AGCTCCCACT GTGTCCGGTT CGTGCCCCAG 
GAGATGGGTG TGCACACGGT GAGCGTCAAG TACCGAGGGC AGCACGTGAC CGGAAGCCCG 
TTCCAGTTCA CAGTTGGGCC CCTCGGGGAA GGGGGTGCCC AGAAAGTCCG CGCCGGGGGC 
CCCGGCCTCG AGAGAGGAGA AGCGGGAGTG CCAGCTGAAT TTAGCATTTG GACCAGAGAA 
GCAGGGGCCG GGGGTCTCTC GATTGCTGTC GAAGGACCGA GTAAGGCAGA AATTGCCTTT 
GACGACCACA AAAACGGGTC GTGTGGCGTG TCTTACATTG CTCAAGAACC GGGTAACTAT 
GAGGTGTCGA TCAAGTTCAA CGACGAGCAC ATCCCAGAGA GCCCCTACTT GGTGCCCATC 
ATCGCTCCTT CCGACGACGC CCGCAGACTC ACTGTTATGA GCCTTCAGGA ATCTGGATTA 
AAAGTTAACC AGCCAGCATC CTTTGCGATA AGGCTGAATG GGGCCAAAGG CAAGATCGAT 
GCAAAAGTCC ACAGCCCCTC GGGTGCCGTC GAGGAGTGCC ACGTGTCCGA GCTGGAGCCA 
GATAAGTACG CCGTGCGCTT CATTCCCCAC GAGAACGGCA TTCACACGAT CGACGTCAAG 
TTCAATGGCA GCCACGTGGT TGGAAGCCCT TTTAAAGTTC GAGTCGGGGA TCCAGGACAA 
GCGGGAAATC CAGCTCTCGT CTCTGCCTAT GGAGCTGGCC TGGAAGGGGG GACCACAGGC 
GTCCAATCGG AGTTTTTCAT CAATACCACC AGAGCTGGCC CCGGGACACT GTCTGTTACA 
ATTGAGGGAC CGTCCAAGGT CAAAATGGAC TGCCAGGAGA CTCCAGAAGG TTACAAAGTC 
ATGTATACAC CGATGGCTCC TGGGAATTAT CTGATCGGTG TGAAATACGG CGGACCCAAC 
CACATCGTTG GCAGCCCTTT CAAGGCCAAG GTGACAGGTC AACGCCTCGT CAGCCCGTGC 
TCTGCCAACG AGACATCTTC CATCCTGGTT GAGTCGGTGA CCAGATCCTC CACAGAAACC 
TGCTACAGCG CCGTCCCCAA AGCTTCTTCA GACGCCAGCA AAGTGACTTC AAAGGGAGCA 
GGGCTCGTGA AAGCTTTCGT GGGCCAGAAG AGCAGCTTCC TGGTCGACTG CAGCAAGGCG 
GGTTCCAATA TGTTACTGAT TGGTGTCCAT GGTCCCACCA CGCCTTGTGA AGAAGTGTCC 
ATGAAACATG TAGGAAACCA GCAGTATAAT GTGACTTATG TTGTCAAGGA GAAGGGGGAT 
TATGTTTTGG CTGTCAAGTG GGGTGAGGAG CACATTCCGG GAAGTCCCTT CCATATCACA 
GTACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_031953198.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809058.1 Sarcophilus harrisii filamin B (FLNB), transcript variant X3, mRNA.

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CloneID	OSa219000
Clone ID Related Accession (Same CDS sequence)	XM_031953198.1
Accession Version	XM_031953198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	filamin-B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG CGGACGAGGT GGACTCCCAG GCCCGGCGCA GCCCATTGAA GGCCTTGTCA 
GATTTCTTTA GAGATGACCA GCAGGGTGAC TTTAATGAGA CAGGCCTGGT GGAACCGGTG 
ACTGTGACGG ACAACGGCGA TGGCACTCAC ACCGTCACCT ACACCCCCTC GCAGGAAGGC 
CCGTACATGC TGTCTGTCAA GTACGCCGAT GAGGACGTCC CCCACAGTCC CTTTAAGGTC 
AAGGTCCTCC CCACGTATGA CGCTAGCAAA GTGACCGCAA GCGGCCCCGG TCTCAGCTCC 
TATGGGGTCC CGGCCAGCTT GCCCGTGGAG TTTGCAATCG ATGCCAAAAA AGCAGGAATT 
GGGCCCCTCG CTGTCCAGAT AACGGACCAA GAAGGAAAGC CCAAAAAGGC CGACGTCCAC 
GACAACAAAG ATGGCACCTA CTCCGTGACC TACCTCCCAG ACAAGACGGG CCGCTATCTG 
ATCGGGGTCA CGTACGGCGG GGAAAACATC CCGTCTTCTC CTTACCGCAT CCGTGCAACG 
CCGACTGGAG ACGCCAGCAA GTGCATGGCC ACAGGTCCTG GGATCGCACC CACTGTCAAA 
ACTGGAGAGG AAGTTGGCTT TGTGGTGGAT GCTAAGACTG CGGGGAAAGG CAAGGTGACC 
TGCACGATCC TGACCCCGGA CGGCACGGAA GTGGAAGCCG AGGTCATCGA GAACGAGGAT 
GGGACCTACG ACATCTTCTA CACGGCAGCC AAACCCGGGA CCTACGTCAT CTATGTGCGC 
TTTGGGGGCG TGGATATCCC CAACAGCCCC TTCACCGTCA TGGTGACGGA AGAAGCCTAC 
GTTCCCGTCA GTGACATGAA TGGCCTGGGA TTTAAGCCCT TTGACCTGGT CATTCCCTTT 
GCTGTACGGA AGGGAGAAGT AACCGGGGAA GTGCACATGC CCTCCGGGAA GACGGCTGCC 
CCCGAGATCG TCGACAACAA AGATGGCACC GTCACGGTTC GCTACGCGCC CACCGAGGTC 
GGCCTGCACG AGATGCACAT CAAGTACATG GGGAACCACA TCCCCGAGAG CCCGCTCCAG 
TTCTATGTGA ACTACCCAAA TAGCGGCAGC GTGTCGGCGT ACGGGCCCGG CCTGGTGTAC 
GGCGTCGCCA ACAAGACGGC CACCTTCACC ATCGTCACAG AGGATGCGGG AGAGGGGGGC 
CTGGACCTGG CCATCGAGGG GCCTTCCAAA GCTGAAATCA GCTGCATCGA CAACAAGGAC 
GGCACCTGCA CGGTGACCTA CCTGCCCACA CTCCCCGGGG ACTACAGCAT CCTGGTGAAG 
TACAATGACA AGCACATACC CGGCAGCCCC TTCACCGCCA AGATCACAGA TAACAACAGG 
AGGTGTTCCC AAGTGAAGCT GGGCTCGGCT GCCGACTTCC TGCTGGACAT CAGCGAGACT 
GACCTGAGCA CACTGACCGC CAGCATCAAA GCTCCGTCCG GGCGAGATGA GCCTTGCCTC 
TTGAAGAGGC TCCCAAACAA CCACATTGGC ATTTCTTTCA TCCCCCGGGA AGTCGGGGAA 
CACCTGGTCA GCATTAAGAA AAATGGCAGC CACGTGGCCA ACAGCCCGGT ATCCATCATG 
GTGGTTCAGT CCGAGATCGG AGACGCCCGG AGGGCCAAGG TCTCAGGCCG GGGCTTGAGT 
GAAGGGCGCA CTTTTGAAAT GTCCGACTTC ATTGTTGACA CAAGGGATGC AGGTTACGGT 
GGCATATCCC TGGCGGTGGA AGGCCCCAGC AAGGTGGACA TCCAGACGGA AGACCTTGAA 
GATGGCACCT GTAAAGTGTC CTACTTCCCC ACGGTGCCGG GGGTGTATAT TGTATCCACC 
AAGTTTGCAG ATGAACACGT GCCAGGGAGT CCCTTCACTG TGAAAATCAG CGGAGAAGGC 
AGGGTCAAGG AAAGCATCAC GCGCCCAAGT CGGGCACCGT CGGTGGCGAC CGTTGGGAGC 
ATCTGTGACC TCAACCTGAA AATCCCAGAA ATAGACAGTG CAGACATGTC GGCCCACGTC 
ACCAGCCCCT CTGGCCGCAC GACGGAGGCC GAGATCGTGC CCGTAGACAA GAGCTCCCAC 
TGTGTCCGGT TCGTGCCCCA GGAGATGGGT GTGCACACGG TGAGCGTCAA GTACCGAGGG 
CAGCACGTGA CCGGAAGCCC GTTCCAGTTC ACAGTTGGGC CCCTCGGGGA AGGGGGTGCC 
CAGAAAGTCC GCGCCGGGGG CCCCGGCCTC GAGAGAGGAG AAGCGGGAGT GCCAGCTGAA 
TTTAGCATTT GGACCAGAGA AGCAGGGGCC GGGGGTCTCT CGATTGCTGT CGAAGGACCG 
AGTAAGGCAG AAATTGCCTT TGACGACCAC AAAAACGGGT CGTGTGGCGT GTCTTACATT 
GCTCAAGAAC CGGGTAACTA TGAGGTGTCG ATCAAGTTCA ACGACGAGCA CATCCCAGAG 
AGCCCCTACT TGGTGCCCAT CATCGCTCCT TCCGACGACG CCCGCAGACT CACTGTTATG 
AGCCTTCAGG AATCTGGATT AAAAGTTAAC CAGCCAGCAT CCTTTGCGAT AAGGCTGAAT 
GGGGCCAAAG GCAAGATCGA TGCAAAAGTC CACAGCCCCT CGGGTGCCGT CGAGGAGTGC 
CACGTGTCCG AGCTGGAGCC AGATAAGTAC GCCGTGCGCT TCATTCCCCA CGAGAACGGC 
ATTCACACGA TCGACGTCAA GTTCAATGGC AGCCACGTGG TTGGAAGCCC TTTTAAAGTT 
CGAGTCGGGG ATCCAGGACA AGCGGGAAAT CCAGCTCTCG TCTCTGCCTA TGGAGCTGGC 
CTGGAAGGGG GGACCACAGG CGTCCAATCG GAGTTTTTCA TCAATACCAC CAGAGCTGGC 
CCCGGGACAC TGTCTGTTAC AATTGAGGGA CCGTCCAAGG TCAAAATGGA CTGCCAGGAG 
ACTCCAGAAG GTTACAAAGT CATGTATACA CCGATGGCTC CTGGGAATTA TCTGATCGGT 
GTGAAATACG GCGGACCCAA CCACATCGTT GGCAGCCCTT TCAAGGCCAA GGTGACAGGT 
CAACGCCTCG TCAGCCCGTG CTCTGCCAAC GAGACATCTT CCATCCTGGT TGAGTCGGTG 
ACCAGATCCT CCACAGAAAC CTGCTACAGC GCCGTCCCCA AAGCTTCTTC AGACGCCAGC 
AAAGTGACTT CAAAGGGAGC AGGGCTCGTG AAAGCTTTCG TGGGCCAGAA GAGCAGCTTC 
CTGGTCGACT GCAGCAAGGC GGGTTCCAAT ATGTTACTGA TTGGTGTCCA TGGTCCCACC 
ACGCCTTGTG AAGAAGTGTC CATGAAACAT GTAGGAAACC AGCAGTATAA TGTGACTTAT 
GTTGTCAAGG AGAAGGGGGA TTATGTTTTG GCTGTCAAGT GGGGTGAGGA GCACATTCCG 
GGAAGTCCCT TCCATATCAC AGTACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809058.1
CDS	150..7979
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLELESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPSKPVDNAREAMQQADDWLGVPQVITPEEIIHPNVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVAGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEATGNIANKATFFDIYTAGAGVGDIEVEVEDPQGKKTVEMVIEDKGNQVYRCAYKPIQAGPHVVKVSFAGESIPKSPFTVNVGEACNPNACRASGRGLQPKGVRIRETADFKVDTKSAGSGELSVTVRGPKGLEELVKQKGFANGVYAFEYYPGTPGKYVVAITWGGHNIPKSPFEVQVGPEAGVQKVRAWGPGLHGGIAGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPYMAYIQPASGDFNPDLVQAYGPGLEKTGCIVNNLAEFTVDPKDAGKAPLKIYAQDGEGSPIEIQMKGKMDGTYACFYTPLKPIKHTISVVWGGVNIPKSPYRVGVGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCTEAGEGDVSVGIKCDAGVLGPDEEDVDFDIIHNANDTFTVKYAPPAPGRYTIKVLFASKEIPASPFRVKVDPSHDASKVRAEGPGLSKAGVENGKPTHFTVFTKGAGKAPLDVQFSGPQAGDVVHDLDIIDNYDYSHTVKYTPVQQGSTQVLVTYGGDPIPRSPFVVEVAAPLDLSKIKLNGLENRVEVGKDQEFVVDTKGAGGQGKLDVTILSPARQVVPCLVASVPGKDCSTAKYIPKEEGLYAVDVSYDGNPIPGSPFTVEASLPPDPTKVKAHGPGLEAGFVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDASKVVASGPGLEHGKVGEAGVLTVDCSEAGRGVLGMEAVSDDGVRADVHIQNNKDGTYAVTYVPLAAGMYTLSLNYGGEQVPGFPARVKVEPAVDTSKIKVYGPGIEGKDVFREATTSFTVDSRPLTKVGGEHVKASIANPSGAFTDYLLKDNADGTYEVEFTPFEKGFHTVEVTYDDVPVPNSPFQVAVTEGCHPSRVQAQGPGLKEGFTHKPNPFTVVTRGAGIGGLGITVEGPSESKISCKDNKDGSCSAEYIPFVPGDYDVNITYGNEHVPGSPFRIPVKDVIDPSKVKLAGPGLGAGVRAKVPQSFTVDTSQAGLAPLEVLVLGPRADEVDSQARRSPLKALSDFFRDDQQGDFNETGLVEPVTVTDNGDGTHTVTYTPSQEGPYMLSVKYADEDVPHSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVEFAIDAKKAGIGPLAVQITDQEGKPKKADVHDNKDGTYSVTYLPDKTGRYLIGVTYGGENIPSSPYRIRATPTGDASKCMATGPGIAPTVKTGEEVGFVVDAKTAGKGKVTCTILTPDGTEVEAEVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEVTGEVHMPSGKTAAPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGNHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDNNRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGSHVANSPVSIMVVQSEIGDARRAKVSGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRPSRAPSVATVGSICDLNLKIPEIDSADMSAHVTSPSGRTTEAEIVPVDKSSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAQKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYLVPIIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGDPGQAGNPALVSAYGAGLEGGTTGVQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPCSANETSSILVESVTRSSTETCYSAVPKASSDASKVTSKGAGLVKAFVGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKEKGDYVLAVKWGEEHIPGSPFHITVP

Target ORF information:

RefSeq Version	XM_031953198.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii filamin B (FLNB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953198.1

ORF Insert Sequence:

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG CGGACGAGGT GGACTCCCAG GCCCGGCGCA GCCCATTGAA GGCCTTGTCA 
GATTTCTTTA GAGATGACCA GCAGGGTGAC TTTAATGAGA CAGGCCTGGT GGAACCGGTG 
ACTGTGACGG ACAACGGCGA TGGCACTCAC ACCGTCACCT ACACCCCCTC GCAGGAAGGC 
CCGTACATGC TGTCTGTCAA GTACGCCGAT GAGGACGTCC CCCACAGTCC CTTTAAGGTC 
AAGGTCCTCC CCACGTATGA CGCTAGCAAA GTGACCGCAA GCGGCCCCGG TCTCAGCTCC 
TATGGGGTCC CGGCCAGCTT GCCCGTGGAG TTTGCAATCG ATGCCAAAAA AGCAGGAATT 
GGGCCCCTCG CTGTCCAGAT AACGGACCAA GAAGGAAAGC CCAAAAAGGC CGACGTCCAC 
GACAACAAAG ATGGCACCTA CTCCGTGACC TACCTCCCAG ACAAGACGGG CCGCTATCTG 
ATCGGGGTCA CGTACGGCGG GGAAAACATC CCGTCTTCTC CTTACCGCAT CCGTGCAACG 
CCGACTGGAG ACGCCAGCAA GTGCATGGCC ACAGGTCCTG GGATCGCACC CACTGTCAAA 
ACTGGAGAGG AAGTTGGCTT TGTGGTGGAT GCTAAGACTG CGGGGAAAGG CAAGGTGACC 
TGCACGATCC TGACCCCGGA CGGCACGGAA GTGGAAGCCG AGGTCATCGA GAACGAGGAT 
GGGACCTACG ACATCTTCTA CACGGCAGCC AAACCCGGGA CCTACGTCAT CTATGTGCGC 
TTTGGGGGCG TGGATATCCC CAACAGCCCC TTCACCGTCA TGGTGACGGA AGAAGCCTAC 
GTTCCCGTCA GTGACATGAA TGGCCTGGGA TTTAAGCCCT TTGACCTGGT CATTCCCTTT 
GCTGTACGGA AGGGAGAAGT AACCGGGGAA GTGCACATGC CCTCCGGGAA GACGGCTGCC 
CCCGAGATCG TCGACAACAA AGATGGCACC GTCACGGTTC GCTACGCGCC CACCGAGGTC 
GGCCTGCACG AGATGCACAT CAAGTACATG GGGAACCACA TCCCCGAGAG CCCGCTCCAG 
TTCTATGTGA ACTACCCAAA TAGCGGCAGC GTGTCGGCGT ACGGGCCCGG CCTGGTGTAC 
GGCGTCGCCA ACAAGACGGC CACCTTCACC ATCGTCACAG AGGATGCGGG AGAGGGGGGC 
CTGGACCTGG CCATCGAGGG GCCTTCCAAA GCTGAAATCA GCTGCATCGA CAACAAGGAC 
GGCACCTGCA CGGTGACCTA CCTGCCCACA CTCCCCGGGG ACTACAGCAT CCTGGTGAAG 
TACAATGACA AGCACATACC CGGCAGCCCC TTCACCGCCA AGATCACAGA TAACAACAGG 
AGGTGTTCCC AAGTGAAGCT GGGCTCGGCT GCCGACTTCC TGCTGGACAT CAGCGAGACT 
GACCTGAGCA CACTGACCGC CAGCATCAAA GCTCCGTCCG GGCGAGATGA GCCTTGCCTC 
TTGAAGAGGC TCCCAAACAA CCACATTGGC ATTTCTTTCA TCCCCCGGGA AGTCGGGGAA 
CACCTGGTCA GCATTAAGAA AAATGGCAGC CACGTGGCCA ACAGCCCGGT ATCCATCATG 
GTGGTTCAGT CCGAGATCGG AGACGCCCGG AGGGCCAAGG TCTCAGGCCG GGGCTTGAGT 
GAAGGGCGCA CTTTTGAAAT GTCCGACTTC ATTGTTGACA CAAGGGATGC AGGTTACGGT 
GGCATATCCC TGGCGGTGGA AGGCCCCAGC AAGGTGGACA TCCAGACGGA AGACCTTGAA 
GATGGCACCT GTAAAGTGTC CTACTTCCCC ACGGTGCCGG GGGTGTATAT TGTATCCACC 
AAGTTTGCAG ATGAACACGT GCCAGGGAGT CCCTTCACTG TGAAAATCAG CGGAGAAGGC 
AGGGTCAAGG AAAGCATCAC GCGCCCAAGT CGGGCACCGT CGGTGGCGAC CGTTGGGAGC 
ATCTGTGACC TCAACCTGAA AATCCCAGAA ATAGACAGTG CAGACATGTC GGCCCACGTC 
ACCAGCCCCT CTGGCCGCAC GACGGAGGCC GAGATCGTGC CCGTAGACAA GAGCTCCCAC 
TGTGTCCGGT TCGTGCCCCA GGAGATGGGT GTGCACACGG TGAGCGTCAA GTACCGAGGG 
CAGCACGTGA CCGGAAGCCC GTTCCAGTTC ACAGTTGGGC CCCTCGGGGA AGGGGGTGCC 
CAGAAAGTCC GCGCCGGGGG CCCCGGCCTC GAGAGAGGAG AAGCGGGAGT GCCAGCTGAA 
TTTAGCATTT GGACCAGAGA AGCAGGGGCC GGGGGTCTCT CGATTGCTGT CGAAGGACCG 
AGTAAGGCAG AAATTGCCTT TGACGACCAC AAAAACGGGT CGTGTGGCGT GTCTTACATT 
GCTCAAGAAC CGGGTAACTA TGAGGTGTCG ATCAAGTTCA ACGACGAGCA CATCCCAGAG 
AGCCCCTACT TGGTGCCCAT CATCGCTCCT TCCGACGACG CCCGCAGACT CACTGTTATG 
AGCCTTCAGG AATCTGGATT AAAAGTTAAC CAGCCAGCAT CCTTTGCGAT AAGGCTGAAT 
GGGGCCAAAG GCAAGATCGA TGCAAAAGTC CACAGCCCCT CGGGTGCCGT CGAGGAGTGC 
CACGTGTCCG AGCTGGAGCC AGATAAGTAC GCCGTGCGCT TCATTCCCCA CGAGAACGGC 
ATTCACACGA TCGACGTCAA GTTCAATGGC AGCCACGTGG TTGGAAGCCC TTTTAAAGTT 
CGAGTCGGGG ATCCAGGACA AGCGGGAAAT CCAGCTCTCG TCTCTGCCTA TGGAGCTGGC 
CTGGAAGGGG GGACCACAGG CGTCCAATCG GAGTTTTTCA TCAATACCAC CAGAGCTGGC 
CCCGGGACAC TGTCTGTTAC AATTGAGGGA CCGTCCAAGG TCAAAATGGA CTGCCAGGAG 
ACTCCAGAAG GTTACAAAGT CATGTATACA CCGATGGCTC CTGGGAATTA TCTGATCGGT 
GTGAAATACG GCGGACCCAA CCACATCGTT GGCAGCCCTT TCAAGGCCAA GGTGACAGGT 
CAACGCCTCG TCAGCCCGTG CTCTGCCAAC GAGACATCTT CCATCCTGGT TGAGTCGGTG 
ACCAGATCCT CCACAGAAAC CTGCTACAGC GCCGTCCCCA AAGCTTCTTC AGACGCCAGC 
AAAGTGACTT CAAAGGGAGC AGGGCTCGTG AAAGCTTTCG TGGGCCAGAA GAGCAGCTTC 
CTGGTCGACT GCAGCAAGGC GGGTTCCAAT ATGTTACTGA TTGGTGTCCA TGGTCCCACC 
ACGCCTTGTG AAGAAGTGTC CATGAAACAT GTAGGAAACC AGCAGTATAA TGTGACTTAT 
GTTGTCAAGG AGAAGGGGGA TTATGTTTTG GCTGTCAAGT GGGGTGAGGA GCACATTCCG 
GGAAGTCCCT TCCATATCAC AGTACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_031953204.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809064.1 Sarcophilus harrisii filamin B (FLNB), transcript variant X4, mRNA.

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CloneID	OSa219001
Clone ID Related Accession (Same CDS sequence)	XM_031953204.1
Accession Version	XM_031953204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	filamin-B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG GCCTGGTGGA ACCGGTGACT GTGACGGACA ACGGCGATGG CACTCACACC 
GTCACCTACA CCCCCTCGCA GGAAGGCCCG TACATGCTGT CTGTCAAGTA CGCCGATGAG 
GACGTCCCCC ACAGTCCCTT TAAGGTCAAG GTCCTCCCCA CGTATGACGC TAGCAAAGTG 
ACCGCAAGCG GCCCCGGTCT CAGCTCCTAT GGGGTCCCGG CCAGCTTGCC CGTGGAGTTT 
GCAATCGATG CCAAAAAAGC AGGAATTGGG CCCCTCGCTG TCCAGATAAC GGACCAAGAA 
GGAAAGCCCA AAAAGGCCGA CGTCCACGAC AACAAAGATG GCACCTACTC CGTGACCTAC 
CTCCCAGACA AGACGGGCCG CTATCTGATC GGGGTCACGT ACGGCGGGGA AAACATCCCG 
TCTTCTCCTT ACCGCATCCG TGCAACGCCG ACTGGAGACG CCAGCAAGTG CATGGCCACA 
GGTCCTGGGA TCGCACCCAC TGTCAAAACT GGAGAGGAAG TTGGCTTTGT GGTGGATGCT 
AAGACTGCGG GGAAAGGCAA GGTGACCTGC ACGATCCTGA CCCCGGACGG CACGGAAGTG 
GAAGCCGAGG TCATCGAGAA CGAGGATGGG ACCTACGACA TCTTCTACAC GGCAGCCAAA 
CCCGGGACCT ACGTCATCTA TGTGCGCTTT GGGGGCGTGG ATATCCCCAA CAGCCCCTTC 
ACCGTCATGG CCACAGAGGA GGACGTTGCC GTAATGAAGG AGGCGCCGGT GACGGAAGAA 
GCCTACGTTC CCGTCAGTGA CATGAATGGC CTGGGATTTA AGCCCTTTGA CCTGGTCATT 
CCCTTTGCTG TACGGAAGGG AGAAGTAACC GGGGAAGTGC ACATGCCCTC CGGGAAGACG 
GCTGCCCCCG AGATCGTCGA CAACAAAGAT GGCACCGTCA CGGTTCGCTA CGCGCCCACC 
GAGGTCGGCC TGCACGAGAT GCACATCAAG TACATGGGGA ACCACATCCC CGAGAGCCCG 
CTCCAGTTCT ATGTGAACTA CCCAAATAGC GGCAGCGTGT CGGCGTACGG GCCCGGCCTG 
GTGTACGGCG TCGCCAACAA GACGGCCACC TTCACCATCG TCACAGAGGA TGCGGGAGAG 
GGGGGCCTGG ACCTGGCCAT CGAGGGGCCT TCCAAAGCTG AAATCAGCTG CATCGACAAC 
AAGGACGGCA CCTGCACGGT GACCTACCTG CCCACACTCC CCGGGGACTA CAGCATCCTG 
GTGAAGTACA ATGACAAGCA CATACCCGGC AGCCCCTTCA CCGCCAAGAT CACAGATAAC 
AACAGGAGGT GTTCCCAAGT GAAGCTGGGC TCGGCTGCCG ACTTCCTGCT GGACATCAGC 
GAGACTGACC TGAGCACACT GACCGCCAGC ATCAAAGCTC CGTCCGGGCG AGATGAGCCT 
TGCCTCTTGA AGAGGCTCCC AAACAACCAC ATTGGCATTT CTTTCATCCC CCGGGAAGTC 
GGGGAACACC TGGTCAGCAT TAAGAAAAAT GGCAGCCACG TGGCCAACAG CCCGGTATCC 
ATCATGGTGG TTCAGTCCGA GATCGGAGAC GCCCGGAGGG CCAAGGTCTC AGGCCGGGGC 
TTGAGTGAAG GGCGCACTTT TGAAATGTCC GACTTCATTG TTGACACAAG GGATGCAGGT 
TACGGTGGCA TATCCCTGGC GGTGGAAGGC CCCAGCAAGG TGGACATCCA GACGGAAGAC 
CTTGAAGATG GCACCTGTAA AGTGTCCTAC TTCCCCACGG TGCCGGGGGT GTATATTGTA 
TCCACCAAGT TTGCAGATGA ACACGTGCCA GGGAGTCCCT TCACTGTGAA AATCAGCGGA 
GAAGGCAGGG TCAAGGAAAG CATCACGCGC CCAAGTCGGG CACCGTCGGT GGCGACCGTT 
GGGAGCATCT GTGACCTCAA CCTGAAAATC CCAGAAATAG ACAGTGCAGA CATGTCGGCC 
CACGTCACCA GCCCCTCTGG CCGCACGACG GAGGCCGAGA TCGTGCCCGT AGACAAGAGC 
TCCCACTGTG TCCGGTTCGT GCCCCAGGAG ATGGGTGTGC ACACGGTGAG CGTCAAGTAC 
CGAGGGCAGC ACGTGACCGG AAGCCCGTTC CAGTTCACAG TTGGGCCCCT CGGGGAAGGG 
GGTGCCCAGA AAGTCCGCGC CGGGGGCCCC GGCCTCGAGA GAGGAGAAGC GGGAGTGCCA 
GCTGAATTTA GCATTTGGAC CAGAGAAGCA GGGGCCGGGG GTCTCTCGAT TGCTGTCGAA 
GGACCGAGTA AGGCAGAAAT TGCCTTTGAC GACCACAAAA ACGGGTCGTG TGGCGTGTCT 
TACATTGCTC AAGAACCGGG TAACTATGAG GTGTCGATCA AGTTCAACGA CGAGCACATC 
CCAGAGAGCC CCTACTTGGT GCCCATCATC GCTCCTTCCG ACGACGCCCG CAGACTCACT 
GTTATGAGCC TTCAGGAATC TGGATTAAAA GTTAACCAGC CAGCATCCTT TGCGATAAGG 
CTGAATGGGG CCAAAGGCAA GATCGATGCA AAAGTCCACA GCCCCTCGGG TGCCGTCGAG 
GAGTGCCACG TGTCCGAGCT GGAGCCAGAT AAGTACGCCG TGCGCTTCAT TCCCCACGAG 
AACGGCATTC ACACGATCGA CGTCAAGTTC AATGGCAGCC ACGTGGTTGG AAGCCCTTTT 
AAAGTTCGAG TCGGGGATCC AGGACAAGCG GGAAATCCAG CTCTCGTCTC TGCCTATGGA 
GCTGGCCTGG AAGGGGGGAC CACAGGCGTC CAATCGGAGT TTTTCATCAA TACCACCAGA 
GCTGGCCCCG GGACACTGTC TGTTACAATT GAGGGACCGT CCAAGGTCAA AATGGACTGC 
CAGGAGACTC CAGAAGGTTA CAAAGTCATG TATACACCGA TGGCTCCTGG GAATTATCTG 
ATCGGTGTGA AATACGGCGG ACCCAACCAC ATCGTTGGCA GCCCTTTCAA GGCCAAGGTG 
ACAGGTCAAC GCCTCGTCAG CCCGTGCTCT GCCAACGAGA CATCTTCCAT CCTGGTTGAG 
TCGGTGACCA GATCCTCCAC AGAAACCTGC TACAGCGCCG TCCCCAAAGC TTCTTCAGAC 
GCCAGCAAAG TGACTTCAAA GGGAGCAGGG CTCGTGAAAG CTTTCGTGGG CCAGAAGAGC 
AGCTTCCTGG TCGACTGCAG CAAGGCGGGT TCCAATATGT TACTGATTGG TGTCCATGGT 
CCCACCACGC CTTGTGAAGA AGTGTCCATG AAACATGTAG GAAACCAGCA GTATAATGTG 
ACTTATGTTG TCAAGGAGAA GGGGGATTAT GTTTTGGCTG TCAAGTGGGG TGAGGAGCAC 
ATTCCGGGAA GTCCCTTCCA TATCACAGTA CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809064.1
CDS	1..7776
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLELESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPSKPVDNAREAMQQADDWLGVPQVITPEEIIHPNVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVAGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEATGNIANKATFFDIYTAGAGVGDIEVEVEDPQGKKTVEMVIEDKGNQVYRCAYKPIQAGPHVVKVSFAGESIPKSPFTVNVGEACNPNACRASGRGLQPKGVRIRETADFKVDTKSAGSGELSVTVRGPKGLEELVKQKGFANGVYAFEYYPGTPGKYVVAITWGGHNIPKSPFEVQVGPEAGVQKVRAWGPGLHGGIAGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPYMAYIQPASGDFNPDLVQAYGPGLEKTGCIVNNLAEFTVDPKDAGKAPLKIYAQDGEGSPIEIQMKGKMDGTYACFYTPLKPIKHTISVVWGGVNIPKSPYRVGVGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCTEAGEGDVSVGIKCDAGVLGPDEEDVDFDIIHNANDTFTVKYAPPAPGRYTIKVLFASKEIPASPFRVKVDPSHDASKVRAEGPGLSKAGVENGKPTHFTVFTKGAGKAPLDVQFSGPQAGDVVHDLDIIDNYDYSHTVKYTPVQQGSTQVLVTYGGDPIPRSPFVVEVAAPLDLSKIKLNGLENRVEVGKDQEFVVDTKGAGGQGKLDVTILSPARQVVPCLVASVPGKDCSTAKYIPKEEGLYAVDVSYDGNPIPGSPFTVEASLPPDPTKVKAHGPGLEAGFVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDASKVVASGPGLEHGKVGEAGVLTVDCSEAGRGVLGMEAVSDDGVRADVHIQNNKDGTYAVTYVPLAAGMYTLSLNYGGEQVPGFPARVKVEPAVDTSKIKVYGPGIEGKDVFREATTSFTVDSRPLTKVGGEHVKASIANPSGAFTDYLLKDNADGTYEVEFTPFEKGFHTVEVTYDDVPVPNSPFQVAVTEGCHPSRVQAQGPGLKEGFTHKPNPFTVVTRGAGIGGLGITVEGPSESKISCKDNKDGSCSAEYIPFVPGDYDVNITYGNEHVPGSPFRIPVKDVIDPSKVKLAGPGLGAGVRAKVPQSFTVDTSQAGLAPLEVLVLGPRGLVEPVTVTDNGDGTHTVTYTPSQEGPYMLSVKYADEDVPHSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVEFAIDAKKAGIGPLAVQITDQEGKPKKADVHDNKDGTYSVTYLPDKTGRYLIGVTYGGENIPSSPYRIRATPTGDASKCMATGPGIAPTVKTGEEVGFVVDAKTAGKGKVTCTILTPDGTEVEAEVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATEEDVAVMKEAPVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEVTGEVHMPSGKTAAPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGNHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDNNRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGSHVANSPVSIMVVQSEIGDARRAKVSGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRPSRAPSVATVGSICDLNLKIPEIDSADMSAHVTSPSGRTTEAEIVPVDKSSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAQKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYLVPIIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGDPGQAGNPALVSAYGAGLEGGTTGVQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPCSANETSSILVESVTRSSTETCYSAVPKASSDASKVTSKGAGLVKAFVGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKEKGDYVLAVKWGEEHIPGSPFHITVP

Target ORF information:

RefSeq Version	XM_031953204.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii filamin B (FLNB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953204.1

ORF Insert Sequence:

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG GCCTGGTGGA ACCGGTGACT GTGACGGACA ACGGCGATGG CACTCACACC 
GTCACCTACA CCCCCTCGCA GGAAGGCCCG TACATGCTGT CTGTCAAGTA CGCCGATGAG 
GACGTCCCCC ACAGTCCCTT TAAGGTCAAG GTCCTCCCCA CGTATGACGC TAGCAAAGTG 
ACCGCAAGCG GCCCCGGTCT CAGCTCCTAT GGGGTCCCGG CCAGCTTGCC CGTGGAGTTT 
GCAATCGATG CCAAAAAAGC AGGAATTGGG CCCCTCGCTG TCCAGATAAC GGACCAAGAA 
GGAAAGCCCA AAAAGGCCGA CGTCCACGAC AACAAAGATG GCACCTACTC CGTGACCTAC 
CTCCCAGACA AGACGGGCCG CTATCTGATC GGGGTCACGT ACGGCGGGGA AAACATCCCG 
TCTTCTCCTT ACCGCATCCG TGCAACGCCG ACTGGAGACG CCAGCAAGTG CATGGCCACA 
GGTCCTGGGA TCGCACCCAC TGTCAAAACT GGAGAGGAAG TTGGCTTTGT GGTGGATGCT 
AAGACTGCGG GGAAAGGCAA GGTGACCTGC ACGATCCTGA CCCCGGACGG CACGGAAGTG 
GAAGCCGAGG TCATCGAGAA CGAGGATGGG ACCTACGACA TCTTCTACAC GGCAGCCAAA 
CCCGGGACCT ACGTCATCTA TGTGCGCTTT GGGGGCGTGG ATATCCCCAA CAGCCCCTTC 
ACCGTCATGG CCACAGAGGA GGACGTTGCC GTAATGAAGG AGGCGCCGGT GACGGAAGAA 
GCCTACGTTC CCGTCAGTGA CATGAATGGC CTGGGATTTA AGCCCTTTGA CCTGGTCATT 
CCCTTTGCTG TACGGAAGGG AGAAGTAACC GGGGAAGTGC ACATGCCCTC CGGGAAGACG 
GCTGCCCCCG AGATCGTCGA CAACAAAGAT GGCACCGTCA CGGTTCGCTA CGCGCCCACC 
GAGGTCGGCC TGCACGAGAT GCACATCAAG TACATGGGGA ACCACATCCC CGAGAGCCCG 
CTCCAGTTCT ATGTGAACTA CCCAAATAGC GGCAGCGTGT CGGCGTACGG GCCCGGCCTG 
GTGTACGGCG TCGCCAACAA GACGGCCACC TTCACCATCG TCACAGAGGA TGCGGGAGAG 
GGGGGCCTGG ACCTGGCCAT CGAGGGGCCT TCCAAAGCTG AAATCAGCTG CATCGACAAC 
AAGGACGGCA CCTGCACGGT GACCTACCTG CCCACACTCC CCGGGGACTA CAGCATCCTG 
GTGAAGTACA ATGACAAGCA CATACCCGGC AGCCCCTTCA CCGCCAAGAT CACAGATAAC 
AACAGGAGGT GTTCCCAAGT GAAGCTGGGC TCGGCTGCCG ACTTCCTGCT GGACATCAGC 
GAGACTGACC TGAGCACACT GACCGCCAGC ATCAAAGCTC CGTCCGGGCG AGATGAGCCT 
TGCCTCTTGA AGAGGCTCCC AAACAACCAC ATTGGCATTT CTTTCATCCC CCGGGAAGTC 
GGGGAACACC TGGTCAGCAT TAAGAAAAAT GGCAGCCACG TGGCCAACAG CCCGGTATCC 
ATCATGGTGG TTCAGTCCGA GATCGGAGAC GCCCGGAGGG CCAAGGTCTC AGGCCGGGGC 
TTGAGTGAAG GGCGCACTTT TGAAATGTCC GACTTCATTG TTGACACAAG GGATGCAGGT 
TACGGTGGCA TATCCCTGGC GGTGGAAGGC CCCAGCAAGG TGGACATCCA GACGGAAGAC 
CTTGAAGATG GCACCTGTAA AGTGTCCTAC TTCCCCACGG TGCCGGGGGT GTATATTGTA 
TCCACCAAGT TTGCAGATGA ACACGTGCCA GGGAGTCCCT TCACTGTGAA AATCAGCGGA 
GAAGGCAGGG TCAAGGAAAG CATCACGCGC CCAAGTCGGG CACCGTCGGT GGCGACCGTT 
GGGAGCATCT GTGACCTCAA CCTGAAAATC CCAGAAATAG ACAGTGCAGA CATGTCGGCC 
CACGTCACCA GCCCCTCTGG CCGCACGACG GAGGCCGAGA TCGTGCCCGT AGACAAGAGC 
TCCCACTGTG TCCGGTTCGT GCCCCAGGAG ATGGGTGTGC ACACGGTGAG CGTCAAGTAC 
CGAGGGCAGC ACGTGACCGG AAGCCCGTTC CAGTTCACAG TTGGGCCCCT CGGGGAAGGG 
GGTGCCCAGA AAGTCCGCGC CGGGGGCCCC GGCCTCGAGA GAGGAGAAGC GGGAGTGCCA 
GCTGAATTTA GCATTTGGAC CAGAGAAGCA GGGGCCGGGG GTCTCTCGAT TGCTGTCGAA 
GGACCGAGTA AGGCAGAAAT TGCCTTTGAC GACCACAAAA ACGGGTCGTG TGGCGTGTCT 
TACATTGCTC AAGAACCGGG TAACTATGAG GTGTCGATCA AGTTCAACGA CGAGCACATC 
CCAGAGAGCC CCTACTTGGT GCCCATCATC GCTCCTTCCG ACGACGCCCG CAGACTCACT 
GTTATGAGCC TTCAGGAATC TGGATTAAAA GTTAACCAGC CAGCATCCTT TGCGATAAGG 
CTGAATGGGG CCAAAGGCAA GATCGATGCA AAAGTCCACA GCCCCTCGGG TGCCGTCGAG 
GAGTGCCACG TGTCCGAGCT GGAGCCAGAT AAGTACGCCG TGCGCTTCAT TCCCCACGAG 
AACGGCATTC ACACGATCGA CGTCAAGTTC AATGGCAGCC ACGTGGTTGG AAGCCCTTTT 
AAAGTTCGAG TCGGGGATCC AGGACAAGCG GGAAATCCAG CTCTCGTCTC TGCCTATGGA 
GCTGGCCTGG AAGGGGGGAC CACAGGCGTC CAATCGGAGT TTTTCATCAA TACCACCAGA 
GCTGGCCCCG GGACACTGTC TGTTACAATT GAGGGACCGT CCAAGGTCAA AATGGACTGC 
CAGGAGACTC CAGAAGGTTA CAAAGTCATG TATACACCGA TGGCTCCTGG GAATTATCTG 
ATCGGTGTGA AATACGGCGG ACCCAACCAC ATCGTTGGCA GCCCTTTCAA GGCCAAGGTG 
ACAGGTCAAC GCCTCGTCAG CCCGTGCTCT GCCAACGAGA CATCTTCCAT CCTGGTTGAG 
TCGGTGACCA GATCCTCCAC AGAAACCTGC TACAGCGCCG TCCCCAAAGC TTCTTCAGAC 
GCCAGCAAAG TGACTTCAAA GGGAGCAGGG CTCGTGAAAG CTTTCGTGGG CCAGAAGAGC 
AGCTTCCTGG TCGACTGCAG CAAGGCGGGT TCCAATATGT TACTGATTGG TGTCCATGGT 
CCCACCACGC CTTGTGAAGA AGTGTCCATG AAACATGTAG GAAACCAGCA GTATAATGTG 
ACTTATGTTG TCAAGGAGAA GGGGGATTAT GTTTTGGCTG TCAAGTGGGG TGAGGAGCAC 
ATTCCGGGAA GTCCCTTCCA TATCACAGTA CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_031953212.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809072.1 Sarcophilus harrisii filamin B (FLNB), transcript variant X5, mRNA.

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CloneID	OSa219002
Clone ID Related Accession (Same CDS sequence)	XM_031953212.1
Accession Version	XM_031953212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	filamin-B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG GCCTGGTGGA ACCGGTGACT GTGACGGACA ACGGCGATGG CACTCACACC 
GTCACCTACA CCCCCTCGCA GGAAGGCCCG TACATGCTGT CTGTCAAGTA CGCCGATGAG 
GACGTCCCCC ACAGTCCCTT TAAGGTCAAG GTCCTCCCCA CGTATGACGC TAGCAAAGTG 
ACCGCAAGCG GCCCCGGTCT CAGCTCCTAT GGGGTCCCGG CCAGCTTGCC CGTGGAGTTT 
GCAATCGATG CCAAAAAAGC AGGAATTGGG CCCCTCGCTG TCCAGATAAC GGACCAAGAA 
GGAAAGCCCA AAAAGGCCGA CGTCCACGAC AACAAAGATG GCACCTACTC CGTGACCTAC 
CTCCCAGACA AGACGGGCCG CTATCTGATC GGGGTCACGT ACGGCGGGGA AAACATCCCG 
TCTTCTCCTT ACCGCATCCG TGCAACGCCG ACTGGAGACG CCAGCAAGTG CATGGCCACA 
GGTCCTGGGA TCGCACCCAC TGTCAAAACT GGAGAGGAAG TTGGCTTTGT GGTGGATGCT 
AAGACTGCGG GGAAAGGCAA GGTGACCTGC ACGATCCTGA CCCCGGACGG CACGGAAGTG 
GAAGCCGAGG TCATCGAGAA CGAGGATGGG ACCTACGACA TCTTCTACAC GGCAGCCAAA 
CCCGGGACCT ACGTCATCTA TGTGCGCTTT GGGGGCGTGG ATATCCCCAA CAGCCCCTTC 
ACCGTCATGG TGACGGAAGA AGCCTACGTT CCCGTCAGTG ACATGAATGG CCTGGGATTT 
AAGCCCTTTG ACCTGGTCAT TCCCTTTGCT GTACGGAAGG GAGAAGTAAC CGGGGAAGTG 
CACATGCCCT CCGGGAAGAC GGCTGCCCCC GAGATCGTCG ACAACAAAGA TGGCACCGTC 
ACGGTTCGCT ACGCGCCCAC CGAGGTCGGC CTGCACGAGA TGCACATCAA GTACATGGGG 
AACCACATCC CCGAGAGCCC GCTCCAGTTC TATGTGAACT ACCCAAATAG CGGCAGCGTG 
TCGGCGTACG GGCCCGGCCT GGTGTACGGC GTCGCCAACA AGACGGCCAC CTTCACCATC 
GTCACAGAGG ATGCGGGAGA GGGGGGCCTG GACCTGGCCA TCGAGGGGCC TTCCAAAGCT 
GAAATCAGCT GCATCGACAA CAAGGACGGC ACCTGCACGG TGACCTACCT GCCCACACTC 
CCCGGGGACT ACAGCATCCT GGTGAAGTAC AATGACAAGC ACATACCCGG CAGCCCCTTC 
ACCGCCAAGA TCACAGATAA CAACAGGAGG TGTTCCCAAG TGAAGCTGGG CTCGGCTGCC 
GACTTCCTGC TGGACATCAG CGAGACTGAC CTGAGCACAC TGACCGCCAG CATCAAAGCT 
CCGTCCGGGC GAGATGAGCC TTGCCTCTTG AAGAGGCTCC CAAACAACCA CATTGGCATT 
TCTTTCATCC CCCGGGAAGT CGGGGAACAC CTGGTCAGCA TTAAGAAAAA TGGCAGCCAC 
GTGGCCAACA GCCCGGTATC CATCATGGTG GTTCAGTCCG AGATCGGAGA CGCCCGGAGG 
GCCAAGGTCT CAGGCCGGGG CTTGAGTGAA GGGCGCACTT TTGAAATGTC CGACTTCATT 
GTTGACACAA GGGATGCAGG TTACGGTGGC ATATCCCTGG CGGTGGAAGG CCCCAGCAAG 
GTGGACATCC AGACGGAAGA CCTTGAAGAT GGCACCTGTA AAGTGTCCTA CTTCCCCACG 
GTGCCGGGGG TGTATATTGT ATCCACCAAG TTTGCAGATG AACACGTGCC AGGGAGTCCC 
TTCACTGTGA AAATCAGCGG AGAAGGCAGG GTCAAGGAAA GCATCACGCG CCCAAGTCGG 
GCACCGTCGG TGGCGACCGT TGGGAGCATC TGTGACCTCA ACCTGAAAAT CCCAGAAATA 
GACAGTGCAG ACATGTCGGC CCACGTCACC AGCCCCTCTG GCCGCACGAC GGAGGCCGAG 
ATCGTGCCCG TAGACAAGAG CTCCCACTGT GTCCGGTTCG TGCCCCAGGA GATGGGTGTG 
CACACGGTGA GCGTCAAGTA CCGAGGGCAG CACGTGACCG GAAGCCCGTT CCAGTTCACA 
GTTGGGCCCC TCGGGGAAGG GGGTGCCCAG AAAGTCCGCG CCGGGGGCCC CGGCCTCGAG 
AGAGGAGAAG CGGGAGTGCC AGCTGAATTT AGCATTTGGA CCAGAGAAGC AGGGGCCGGG 
GGTCTCTCGA TTGCTGTCGA AGGACCGAGT AAGGCAGAAA TTGCCTTTGA CGACCACAAA 
AACGGGTCGT GTGGCGTGTC TTACATTGCT CAAGAACCGG GTAACTATGA GGTGTCGATC 
AAGTTCAACG ACGAGCACAT CCCAGAGAGC CCCTACTTGG TGCCCATCAT CGCTCCTTCC 
GACGACGCCC GCAGACTCAC TGTTATGAGC CTTCAGGAAT CTGGATTAAA AGTTAACCAG 
CCAGCATCCT TTGCGATAAG GCTGAATGGG GCCAAAGGCA AGATCGATGC AAAAGTCCAC 
AGCCCCTCGG GTGCCGTCGA GGAGTGCCAC GTGTCCGAGC TGGAGCCAGA TAAGTACGCC 
GTGCGCTTCA TTCCCCACGA GAACGGCATT CACACGATCG ACGTCAAGTT CAATGGCAGC 
CACGTGGTTG GAAGCCCTTT TAAAGTTCGA GTCGGGGATC CAGGACAAGC GGGAAATCCA 
GCTCTCGTCT CTGCCTATGG AGCTGGCCTG GAAGGGGGGA CCACAGGCGT CCAATCGGAG 
TTTTTCATCA ATACCACCAG AGCTGGCCCC GGGACACTGT CTGTTACAAT TGAGGGACCG 
TCCAAGGTCA AAATGGACTG CCAGGAGACT CCAGAAGGTT ACAAAGTCAT GTATACACCG 
ATGGCTCCTG GGAATTATCT GATCGGTGTG AAATACGGCG GACCCAACCA CATCGTTGGC 
AGCCCTTTCA AGGCCAAGGT GACAGGTCAA CGCCTCGTCA GCCCGTGCTC TGCCAACGAG 
ACATCTTCCA TCCTGGTTGA GTCGGTGACC AGATCCTCCA CAGAAACCTG CTACAGCGCC 
GTCCCCAAAG CTTCTTCAGA CGCCAGCAAA GTGACTTCAA AGGGAGCAGG GCTCGTGAAA 
GCTTTCGTGG GCCAGAAGAG CAGCTTCCTG GTCGACTGCA GCAAGGCGGG TTCCAATATG 
TTACTGATTG GTGTCCATGG TCCCACCACG CCTTGTGAAG AAGTGTCCAT GAAACATGTA 
GGAAACCAGC AGTATAATGT GACTTATGTT GTCAAGGAGA AGGGGGATTA TGTTTTGGCT 
GTCAAGTGGG GTGAGGAGCA CATTCCGGGA AGTCCCTTCC ATATCACAGT ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809072.1
CDS	1..7737
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLELESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPSKPVDNAREAMQQADDWLGVPQVITPEEIIHPNVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVAGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEATGNIANKATFFDIYTAGAGVGDIEVEVEDPQGKKTVEMVIEDKGNQVYRCAYKPIQAGPHVVKVSFAGESIPKSPFTVNVGEACNPNACRASGRGLQPKGVRIRETADFKVDTKSAGSGELSVTVRGPKGLEELVKQKGFANGVYAFEYYPGTPGKYVVAITWGGHNIPKSPFEVQVGPEAGVQKVRAWGPGLHGGIAGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPYMAYIQPASGDFNPDLVQAYGPGLEKTGCIVNNLAEFTVDPKDAGKAPLKIYAQDGEGSPIEIQMKGKMDGTYACFYTPLKPIKHTISVVWGGVNIPKSPYRVGVGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCTEAGEGDVSVGIKCDAGVLGPDEEDVDFDIIHNANDTFTVKYAPPAPGRYTIKVLFASKEIPASPFRVKVDPSHDASKVRAEGPGLSKAGVENGKPTHFTVFTKGAGKAPLDVQFSGPQAGDVVHDLDIIDNYDYSHTVKYTPVQQGSTQVLVTYGGDPIPRSPFVVEVAAPLDLSKIKLNGLENRVEVGKDQEFVVDTKGAGGQGKLDVTILSPARQVVPCLVASVPGKDCSTAKYIPKEEGLYAVDVSYDGNPIPGSPFTVEASLPPDPTKVKAHGPGLEAGFVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDASKVVASGPGLEHGKVGEAGVLTVDCSEAGRGVLGMEAVSDDGVRADVHIQNNKDGTYAVTYVPLAAGMYTLSLNYGGEQVPGFPARVKVEPAVDTSKIKVYGPGIEGKDVFREATTSFTVDSRPLTKVGGEHVKASIANPSGAFTDYLLKDNADGTYEVEFTPFEKGFHTVEVTYDDVPVPNSPFQVAVTEGCHPSRVQAQGPGLKEGFTHKPNPFTVVTRGAGIGGLGITVEGPSESKISCKDNKDGSCSAEYIPFVPGDYDVNITYGNEHVPGSPFRIPVKDVIDPSKVKLAGPGLGAGVRAKVPQSFTVDTSQAGLAPLEVLVLGPRGLVEPVTVTDNGDGTHTVTYTPSQEGPYMLSVKYADEDVPHSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVEFAIDAKKAGIGPLAVQITDQEGKPKKADVHDNKDGTYSVTYLPDKTGRYLIGVTYGGENIPSSPYRIRATPTGDASKCMATGPGIAPTVKTGEEVGFVVDAKTAGKGKVTCTILTPDGTEVEAEVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEVTGEVHMPSGKTAAPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGNHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDNNRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGSHVANSPVSIMVVQSEIGDARRAKVSGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRPSRAPSVATVGSICDLNLKIPEIDSADMSAHVTSPSGRTTEAEIVPVDKSSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAQKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYLVPIIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGDPGQAGNPALVSAYGAGLEGGTTGVQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPCSANETSSILVESVTRSSTETCYSAVPKASSDASKVTSKGAGLVKAFVGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKEKGDYVLAVKWGEEHIPGSPFHITVP

Target ORF information:

RefSeq Version	XM_031953212.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii filamin B (FLNB), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953212.1

ORF Insert Sequence:

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG GCCTGGTGGA ACCGGTGACT GTGACGGACA ACGGCGATGG CACTCACACC 
GTCACCTACA CCCCCTCGCA GGAAGGCCCG TACATGCTGT CTGTCAAGTA CGCCGATGAG 
GACGTCCCCC ACAGTCCCTT TAAGGTCAAG GTCCTCCCCA CGTATGACGC TAGCAAAGTG 
ACCGCAAGCG GCCCCGGTCT CAGCTCCTAT GGGGTCCCGG CCAGCTTGCC CGTGGAGTTT 
GCAATCGATG CCAAAAAAGC AGGAATTGGG CCCCTCGCTG TCCAGATAAC GGACCAAGAA 
GGAAAGCCCA AAAAGGCCGA CGTCCACGAC AACAAAGATG GCACCTACTC CGTGACCTAC 
CTCCCAGACA AGACGGGCCG CTATCTGATC GGGGTCACGT ACGGCGGGGA AAACATCCCG 
TCTTCTCCTT ACCGCATCCG TGCAACGCCG ACTGGAGACG CCAGCAAGTG CATGGCCACA 
GGTCCTGGGA TCGCACCCAC TGTCAAAACT GGAGAGGAAG TTGGCTTTGT GGTGGATGCT 
AAGACTGCGG GGAAAGGCAA GGTGACCTGC ACGATCCTGA CCCCGGACGG CACGGAAGTG 
GAAGCCGAGG TCATCGAGAA CGAGGATGGG ACCTACGACA TCTTCTACAC GGCAGCCAAA 
CCCGGGACCT ACGTCATCTA TGTGCGCTTT GGGGGCGTGG ATATCCCCAA CAGCCCCTTC 
ACCGTCATGG TGACGGAAGA AGCCTACGTT CCCGTCAGTG ACATGAATGG CCTGGGATTT 
AAGCCCTTTG ACCTGGTCAT TCCCTTTGCT GTACGGAAGG GAGAAGTAAC CGGGGAAGTG 
CACATGCCCT CCGGGAAGAC GGCTGCCCCC GAGATCGTCG ACAACAAAGA TGGCACCGTC 
ACGGTTCGCT ACGCGCCCAC CGAGGTCGGC CTGCACGAGA TGCACATCAA GTACATGGGG 
AACCACATCC CCGAGAGCCC GCTCCAGTTC TATGTGAACT ACCCAAATAG CGGCAGCGTG 
TCGGCGTACG GGCCCGGCCT GGTGTACGGC GTCGCCAACA AGACGGCCAC CTTCACCATC 
GTCACAGAGG ATGCGGGAGA GGGGGGCCTG GACCTGGCCA TCGAGGGGCC TTCCAAAGCT 
GAAATCAGCT GCATCGACAA CAAGGACGGC ACCTGCACGG TGACCTACCT GCCCACACTC 
CCCGGGGACT ACAGCATCCT GGTGAAGTAC AATGACAAGC ACATACCCGG CAGCCCCTTC 
ACCGCCAAGA TCACAGATAA CAACAGGAGG TGTTCCCAAG TGAAGCTGGG CTCGGCTGCC 
GACTTCCTGC TGGACATCAG CGAGACTGAC CTGAGCACAC TGACCGCCAG CATCAAAGCT 
CCGTCCGGGC GAGATGAGCC TTGCCTCTTG AAGAGGCTCC CAAACAACCA CATTGGCATT 
TCTTTCATCC CCCGGGAAGT CGGGGAACAC CTGGTCAGCA TTAAGAAAAA TGGCAGCCAC 
GTGGCCAACA GCCCGGTATC CATCATGGTG GTTCAGTCCG AGATCGGAGA CGCCCGGAGG 
GCCAAGGTCT CAGGCCGGGG CTTGAGTGAA GGGCGCACTT TTGAAATGTC CGACTTCATT 
GTTGACACAA GGGATGCAGG TTACGGTGGC ATATCCCTGG CGGTGGAAGG CCCCAGCAAG 
GTGGACATCC AGACGGAAGA CCTTGAAGAT GGCACCTGTA AAGTGTCCTA CTTCCCCACG 
GTGCCGGGGG TGTATATTGT ATCCACCAAG TTTGCAGATG AACACGTGCC AGGGAGTCCC 
TTCACTGTGA AAATCAGCGG AGAAGGCAGG GTCAAGGAAA GCATCACGCG CCCAAGTCGG 
GCACCGTCGG TGGCGACCGT TGGGAGCATC TGTGACCTCA ACCTGAAAAT CCCAGAAATA 
GACAGTGCAG ACATGTCGGC CCACGTCACC AGCCCCTCTG GCCGCACGAC GGAGGCCGAG 
ATCGTGCCCG TAGACAAGAG CTCCCACTGT GTCCGGTTCG TGCCCCAGGA GATGGGTGTG 
CACACGGTGA GCGTCAAGTA CCGAGGGCAG CACGTGACCG GAAGCCCGTT CCAGTTCACA 
GTTGGGCCCC TCGGGGAAGG GGGTGCCCAG AAAGTCCGCG CCGGGGGCCC CGGCCTCGAG 
AGAGGAGAAG CGGGAGTGCC AGCTGAATTT AGCATTTGGA CCAGAGAAGC AGGGGCCGGG 
GGTCTCTCGA TTGCTGTCGA AGGACCGAGT AAGGCAGAAA TTGCCTTTGA CGACCACAAA 
AACGGGTCGT GTGGCGTGTC TTACATTGCT CAAGAACCGG GTAACTATGA GGTGTCGATC 
AAGTTCAACG ACGAGCACAT CCCAGAGAGC CCCTACTTGG TGCCCATCAT CGCTCCTTCC 
GACGACGCCC GCAGACTCAC TGTTATGAGC CTTCAGGAAT CTGGATTAAA AGTTAACCAG 
CCAGCATCCT TTGCGATAAG GCTGAATGGG GCCAAAGGCA AGATCGATGC AAAAGTCCAC 
AGCCCCTCGG GTGCCGTCGA GGAGTGCCAC GTGTCCGAGC TGGAGCCAGA TAAGTACGCC 
GTGCGCTTCA TTCCCCACGA GAACGGCATT CACACGATCG ACGTCAAGTT CAATGGCAGC 
CACGTGGTTG GAAGCCCTTT TAAAGTTCGA GTCGGGGATC CAGGACAAGC GGGAAATCCA 
GCTCTCGTCT CTGCCTATGG AGCTGGCCTG GAAGGGGGGA CCACAGGCGT CCAATCGGAG 
TTTTTCATCA ATACCACCAG AGCTGGCCCC GGGACACTGT CTGTTACAAT TGAGGGACCG 
TCCAAGGTCA AAATGGACTG CCAGGAGACT CCAGAAGGTT ACAAAGTCAT GTATACACCG 
ATGGCTCCTG GGAATTATCT GATCGGTGTG AAATACGGCG GACCCAACCA CATCGTTGGC 
AGCCCTTTCA AGGCCAAGGT GACAGGTCAA CGCCTCGTCA GCCCGTGCTC TGCCAACGAG 
ACATCTTCCA TCCTGGTTGA GTCGGTGACC AGATCCTCCA CAGAAACCTG CTACAGCGCC 
GTCCCCAAAG CTTCTTCAGA CGCCAGCAAA GTGACTTCAA AGGGAGCAGG GCTCGTGAAA 
GCTTTCGTGG GCCAGAAGAG CAGCTTCCTG GTCGACTGCA GCAAGGCGGG TTCCAATATG 
TTACTGATTG GTGTCCATGG TCCCACCACG CCTTGTGAAG AAGTGTCCAT GAAACATGTA 
GGAAACCAGC AGTATAATGT GACTTATGTT GTCAAGGAGA AGGGGGATTA TGTTTTGGCT 
GTCAAGTGGG GTGAGGAGCA CATTCCGGGA AGTCCCTTCC ATATCACAGT ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_031953219.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809079.1 Sarcophilus harrisii filamin B (FLNB), transcript variant X6, mRNA.

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CloneID	OSa219003
Clone ID Related Accession (Same CDS sequence)	XM_031953219.1
Accession Version	XM_031953219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	filamin-B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG GCCTGGTGGA ACCGGTGACT GTGACGGACA ACGGCGATGG CACTCACACC 
GTCACCTACA CCCCCTCGCA GGAAGGCCCG TACATGCTGT CTGTCAAGTA CGCCGATGAG 
GACGTCCCCC ACAGTCCCTT TAAGGTCAAG GTCCTCCCCA CGTATGACGC TAGCAAAGTG 
ACCGCAAGCG GCCCCGGTCT CAGCTCCTAT GGGGTCCCGG CCAGCTTGCC CGTGGAGTTT 
GCAATCGATG CCAAAAAAGC AGGAATTGGG CCCCTCGCTG TCCAGATAAC GGACCAAGAA 
GGAAAGCCCA AAAAGGCCGA CGTCCACGAC AACAAAGATG GCACCTACTC CGTGACCTAC 
CTCCCAGACA AGACGGGCCG CTATCTGATC GGGGTCACGT ACGGCGGGGA AAACATCCCG 
TCTTCTCCTT ACCGCATCCG TGCAACGCCG ACTGGAGACG CCAGCAAGTG CATGGCCACA 
GGTCCTGGGA TCGCACCCAC TGTCAAAACT GGAGAGGAAG TTGGCTTTGT GGTGGATGCT 
AAGACTGCGG GGAAAGGCAA GGTGACCTGC ACGATCCTGA CCCCGGACGG CACGGAAGTG 
GAAGCCGAGG TCATCGAGAA CGAGGATGGG ACCTACGACA TCTTCTACAC GGCAGCCAAA 
CCCGGGACCT ACGTCATCTA TGTGCGCTTT GGGGGCGTGG ATATCCCCAA CAGCCCCTTC 
ACCGTCATGG CCACAGAGGA GGACGTTGCC GTAATGAAGG AGGCGCCGGT AAATGCATGT 
CCCCCCGGCT ACAGTCCTTG GGTGACGGAA GAAGCCTACG TTCCCGTCAG TGACATGAAT 
GGCCTGGGAT TTAAGCCCTT TGACCTGGTC ATTCCCTTTG CTGTACGGAA GGGAGAAGTA 
ACCGGGGAAG TGCACATGCC CTCCGGGAAG ACGGCTGCCC CCGAGATCGT CGACAACAAA 
GATGGCACCG TCACGGTTCG CTACGCGCCC ACCGAGGTCG GCCTGCACGA GATGCACATC 
AAGTACATGG GGAACCACAT CCCCGAGAGC CCGCTCCAGT TCTATGTGAA CTACCCAAAT 
AGCGGCAGCG TGTCGGCGTA CGGGCCCGGC CTGGTGTACG GCGTCGCCAA CAAGACGGCC 
ACCTTCACCA TCGTCACAGA GGATGCGGGA GAGGGGGGCC TGGACCTGGC CATCGAGGGG 
CCTTCCAAAG CTGAAATCAG CTGCATCGAC AACAAGGACG GCACCTGCAC GGTGACCTAC 
CTGCCCACAC TCCCCGGGGA CTACAGCATC CTGGTGAAGT ACAATGACAA GCACATACCC 
GGCAGCCCCT TCACCGCCAA GATCACAGAT AACAACAGGA GGTGTTCCCA AGTGAAGCTG 
GGCTCGGCTG CCGACTTCCT GCTGGACATC AGCGAGACTG ACCTGAGCAC ACTGACCGCC 
AGCATCAAAG CTCCGTCCGG GCGAGATGAG CCTTGCCTCT TGAAGAGGCT CCCAAACAAC 
CACATTGGCA TTTCTTTCAT CCCCCGGGAA GTCGGGGAAC ACCTGGTCAG CATTAAGAAA 
AATGGCAGCC ACGTGGCCAA CAGCCCGGTA TCCATCATGG TGGTTCAGTC CGAGATCGGA 
GACGCCCGGA GGGCCAAGGT CTCAGGCCGG GGCTTGAGTG AAGGGCGCAC TTTTGAAATG 
TCCGACTTCA TTGTTGACAC AAGGGATGCA GGTTACGGTG GCATATCCCT GGCGGTGGAA 
GGCCCCAGCA AGGTGGACAT CCAGACGGAA GACCTTGAAG ATGGCACCTG TAAAGTGTCC 
TACTTCCCCA CGGTGCCGGG GGTGTATATT GTATCCACCA AGTTTGCAGA TGAACACGTG 
CCAGGGAGTC CCTTCACTGT GAAAATCAGC GGAGAAGGCA GGGTCAAGGA AAGCATCACG 
CGCCCAAGTC GGGCACCGTC GGTGGCGACC GTTGGGAGCA TCTGTGACCT CAACCTGAAA 
ATCCCAGAAA TAGACAGTGC AGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCACG 
ACGGAGGCCG AGATCGTGCC CGTAGACAAG AGCTCCCACT GTGTCCGGTT CGTGCCCCAG 
GAGATGGGTG TGCACACGGT GAGCGTCAAG TACCGAGGGC AGCACGTGAC CGGAAGCCCG 
TTCCAGTTCA CAGTTGGGCC CCTCGGGGAA GGGGGTGCCC AGAAAGTCCG CGCCGGGGGC 
CCCGGCCTCG AGAGAGGAGA AGCGGGAGTG CCAGCTGAAT TTAGCATTTG GACCAGAGAA 
GCAGGGGCCG GGGGTCTCTC GATTGCTGTC GAAGGACCGA GTAAGGCAGA AATTGCCTTT 
GACGACCACA AAAACGGGTC GTGTGGCGTG TCTTACATTG CTCAAGAACC GGGTAACTAT 
GAGGTGTCGA TCAAGTTCAA CGACGAGCAC ATCCCAGAGA GCCCCTACTT GGTGCCCATC 
ATCGCTCCTT CCGACGACGC CCGCAGACTC ACTGTTATGA GCCTTCAGGA ATCTGGATTA 
AAAGTTAACC AGCCAGCATC CTTTGCGATA AGGCTGAATG GGGCCAAAGG CAAGATCGAT 
GCAAAAGTCC ACAGCCCCTC GGGTGCCGTC GAGGAGTGCC ACGTGTCCGA GCTGGAGCCA 
GATAAGTACG CCGTGCGCTT CATTCCCCAC GAGAACGGCA TTCACACGAT CGACGTCAAG 
TTCAATGGCA GCCACGTGGT TGGAAGCCCT TTTAAAGTTC GAGTCGGGGA TCCAGGACAA 
GCGGGAAATC CAGCTCTCGT CTCTGCCTAT GGAGCTGGCC TGGAAGGGGG GACCACAGGC 
GTCCAATCGG AGTTTTTCAT CAATACCACC AGAGCTGGCC CCGGGACACT GTCTGTTACA 
ATTGAGGGAC CGTCCAAGGT CAAAATGGAC TGCCAGGAGA CTCCAGAAGG TTACAAAGTC 
ATGTATACAC CGATGGCTCC TGGGAATTAT CTGATCGGTG TGAAATACGG CGGACCCAAC 
CACATCGTTG GCAGCCCTTT CAAGGCCAAG GTGACAGGTC AACGCCTCGT CAGCCCGTGC 
TCTGCCAACG AGACATCTTC CATCCTGGTT GAGTCGGTGA CCAGATCCTC CACAGAAACC 
TGCTACAGCG CCGTCCCCAA AGCTTCTTCA GACGCCAGCA AAGTGACTTC AAAGGGAGCA 
GGGCTCGTGA AAGCTTTCGT GGGCCAGAAG AGCAGCTTCC TGGTCGACTG CAGCAAGGCG 
GGTTCCAATA TGTTACTGAT TGGTGTCCAT GGTCCCACCA CGCCTTGTGA AGAAGTGTCC 
ATGAAACATG TAGGAAACCA GCAGTATAAT GTGACTTATG TTGTCAAGGA GAAGGGGGAT 
TATGTTTTGG CTGTCAAGTG GGGTGAGGAG CACATTCCGG GAAGTCCCTT CCATATCACA 
GTACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809079.1
CDS	150..7958
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLELESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPSKPVDNAREAMQQADDWLGVPQVITPEEIIHPNVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVAGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEATGNIANKATFFDIYTAGAGVGDIEVEVEDPQGKKTVEMVIEDKGNQVYRCAYKPIQAGPHVVKVSFAGESIPKSPFTVNVGEACNPNACRASGRGLQPKGVRIRETADFKVDTKSAGSGELSVTVRGPKGLEELVKQKGFANGVYAFEYYPGTPGKYVVAITWGGHNIPKSPFEVQVGPEAGVQKVRAWGPGLHGGIAGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPYMAYIQPASGDFNPDLVQAYGPGLEKTGCIVNNLAEFTVDPKDAGKAPLKIYAQDGEGSPIEIQMKGKMDGTYACFYTPLKPIKHTISVVWGGVNIPKSPYRVGVGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCTEAGEGDVSVGIKCDAGVLGPDEEDVDFDIIHNANDTFTVKYAPPAPGRYTIKVLFASKEIPASPFRVKVDPSHDASKVRAEGPGLSKAGVENGKPTHFTVFTKGAGKAPLDVQFSGPQAGDVVHDLDIIDNYDYSHTVKYTPVQQGSTQVLVTYGGDPIPRSPFVVEVAAPLDLSKIKLNGLENRVEVGKDQEFVVDTKGAGGQGKLDVTILSPARQVVPCLVASVPGKDCSTAKYIPKEEGLYAVDVSYDGNPIPGSPFTVEASLPPDPTKVKAHGPGLEAGFVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDASKVVASGPGLEHGKVGEAGVLTVDCSEAGRGVLGMEAVSDDGVRADVHIQNNKDGTYAVTYVPLAAGMYTLSLNYGGEQVPGFPARVKVEPAVDTSKIKVYGPGIEGKDVFREATTSFTVDSRPLTKVGGEHVKASIANPSGAFTDYLLKDNADGTYEVEFTPFEKGFHTVEVTYDDVPVPNSPFQVAVTEGCHPSRVQAQGPGLKEGFTHKPNPFTVVTRGAGIGGLGITVEGPSESKISCKDNKDGSCSAEYIPFVPGDYDVNITYGNEHVPGSPFRIPVKDVIDPSKVKLAGPGLGAGVRAKVPQSFTVDTSQAGLAPLEVLVLGPRGLVEPVTVTDNGDGTHTVTYTPSQEGPYMLSVKYADEDVPHSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVEFAIDAKKAGIGPLAVQITDQEGKPKKADVHDNKDGTYSVTYLPDKTGRYLIGVTYGGENIPSSPYRIRATPTGDASKCMATGPGIAPTVKTGEEVGFVVDAKTAGKGKVTCTILTPDGTEVEAEVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATEEDVAVMKEAPVNACPPGYSPWVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEVTGEVHMPSGKTAAPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGNHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDNNRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGSHVANSPVSIMVVQSEIGDARRAKVSGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRPSRAPSVATVGSICDLNLKIPEIDSADMSAHVTSPSGRTTEAEIVPVDKSSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAQKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYLVPIIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGDPGQAGNPALVSAYGAGLEGGTTGVQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPCSANETSSILVESVTRSSTETCYSAVPKASSDASKVTSKGAGLVKAFVGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKEKGDYVLAVKWGEEHIPGSPFHITVP

Target ORF information:

RefSeq Version	XM_031953219.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii filamin B (FLNB), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953219.1

ORF Insert Sequence:

ATGCCCGTGA CGGAGAAGGA CCTGGCCGAG GACGCGCCGT GGAAGAAGAT CCAGCAGAAC 
ACGTTCACGC GCTGGTGCAA CGAGCACCTC AAGTGCGTGA ACAAGCGCAT CGGCAACCTG 
CAGACGGACC TGAGCGACGG GCTGCGGCTG ATCGCGCTGC TCGAGGTGCT CAGCCAGAAG 
CGCATGTACC GCAAGTACCA CCAGCGGCCT ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGAGCTGGAG AGCATCAAGC TCGTGTCCAT AGATAGTAAA 
GCTATTGTTG ATGGAAACTT GAAGCTCATT CTGGGCCTGG TATGGACCCT CATTCTTCAC 
TACTCAATTT CTATGCCTGT TTGGGAAGAT GAAGGAGATG ATGATGCTAA GAAACAAACT 
CCGAAACAGA GGCTGCTGGG CTGGATTCAA AACAAAATCC CTTATTTGCC CATCACCAAC 
TTTAACCAGA ATTGGCAAGA TGGCAAAGCC TTGGGAGCCC TCGTGGATAG CTGTGCCCCA 
GGTCTCTGCC CTGACTGGGA ATCTTGGGAT CCCTCAAAGC CGGTGGACAA TGCCCGGGAA 
GCCATGCAGC AGGCCGATGA CTGGTTGGGG GTCCCACAGG TCATCACTCC TGAAGAAATC 
ATTCACCCCA ATGTGGATGA ACACTCCGTC ATGACTTACC TGTCTCAGTT TCCCAAAGCC 
AAACTCAAGC CGGGAGCCCC TCTCAAACCC AAACTCAACC CAAAGAAGGC CCGGGCCTAT 
GGCCGAGGAA TTGAGCCCAC TGGAAACATG GTGAAGCAGC CGGCCAAGTT TACGGTGGAC 
ACTATCAGTG CTGGACAAGG GGACGTGATG GTTTTTGTGG AAGATCCTGA GGGGAATAAA 
GAGGAGGCAC AAGTGACTCC AGACAGTGAC AAGAACAAGA CTTACTCTGT TGAGTATCTG 
CCTAAAGTCG CTGGACTTCA CAAGGTCACA GTTCTCTTTG CTGGTCAGCA CATCTCCAAG 
AGTCCGTTTG AGGTCAATGT TGACAAGGCC CAGGGCGATG CCAGCAAAGT GACAGCCAAA 
GGACCAGGCT TGGAAGCCAC TGGCAATATT GCTAATAAGG CAACTTTCTT TGACATCTAC 
ACAGCAGGAG CTGGTGTGGG AGACATCGAA GTGGAGGTGG AAGATCCCCA AGGGAAGAAA 
ACGGTAGAAA TGGTCATAGA AGACAAAGGG AATCAGGTCT ACCGCTGTGC CTACAAACCC 
ATTCAGGCTG GTCCCCATGT GGTCAAGGTC TCATTTGCTG GGGAATCTAT TCCCAAAAGC 
CCCTTTACCG TGAATGTTGG CGAAGCCTGC AACCCGAATG CCTGCCGGGC CAGCGGTCGG 
GGCCTGCAGC CCAAGGGCGT GAGGATCCGC GAGACGGCCG ACTTCAAGGT GGACACGAAG 
TCTGCAGGAA GCGGGGAGCT CAGTGTGACT GTGCGGGGGC CCAAGGGGCT GGAGGAGCTT 
GTCAAACAGA AGGGCTTCGC GAACGGCGTC TATGCCTTTG AGTACTACCC GGGAACTCCT 
GGGAAGTACG TGGTGGCCAT CACGTGGGGA GGACACAACA TTCCAAAGAG TCCCTTTGAA 
GTCCAAGTGG GCCCTGAGGC AGGTGTGCAG AAAGTCAGAG CGTGGGGGCC CGGTCTCCAT 
GGAGGCATTG CCGGGAGGTC AGCGGACTTT GTGGTGGAGT CCATTGGCTC GGAAGTTGGA 
TCATTGGGAT TTGCCATTGA AGGCCCCTCC CAGGCCAAGA TTGAATACGA CGACCAGAAC 
GATGGGTCCT GTGACGTGAA GTACTGGCCC AAGGAGCCCG GGGAGTACGC CGTGCACATC 
ATGTGCGATG ATGAGGATAT CAAGGACAGT CCCTATATGG CCTACATACA GCCCGCCTCA 
GGGGACTTCA ACCCAGACCT GGTTCAAGCC TATGGCCCAG GATTAGAGAA GACGGGTTGT 
ATCGTCAACA ACTTGGCCGA ATTCACCGTC GATCCCAAGG ATGCTGGGAA GGCGCCCTTG 
AAGATCTATG CTCAGGATGG AGAAGGCAGT CCCATTGAGA TTCAGATGAA GGGCAAGATG 
GACGGGACCT ACGCCTGTTT TTACACCCCG CTGAAGCCCA TAAAACACAC CATTTCTGTC 
GTCTGGGGAG GAGTGAATAT CCCCAAAAGC CCCTACAGGG TTGGCGTTGG TCAAGGCAGC 
CACCCTCAGA AGGTCAAGGT GTTTGGCCCT GGAGTGGAAA GAAGCGGCCT CAAAGCCAAC 
GAGCCCACTC ACTTCACCGT GGACTGCACC GAGGCAGGGG AAGGCGACGT CAGCGTCGGT 
ATTAAATGTG ACGCCGGGGT TCTGGGCCCG GATGAAGAAG ACGTGGACTT TGACATCATT 
CACAACGCCA ACGACACGTT CACCGTGAAA TACGCGCCGC CTGCCCCTGG CAGATACACC 
ATCAAAGTTC TCTTTGCCTC CAAGGAAATC CCTGCCAGTC CTTTCCGAGT CAAAGTTGAC 
CCCTCCCATG ATGCCAGCAA AGTCAGAGCC GAAGGCCCCG GGCTCAGTAA GGCAGGTGTG 
GAAAATGGCA AGCCGACTCA CTTTACCGTT TTCACCAAGG GGGCGGGGAA AGCCCCGCTC 
GATGTGCAGT TCAGCGGCCC CCAGGCTGGG GACGTCGTCC ACGATCTGGA CATCATCGAC 
AACTATGACT ATTCCCACAC TGTGAAGTAT ACCCCAGTCC AACAGGGCAG CACCCAAGTC 
CTGGTGACCT ACGGCGGAGA TCCGATCCCC AGAAGCCCCT TTGTGGTCGA GGTTGCTGCT 
CCTTTGGATC TGAGCAAGAT AAAGCTCAAT GGGCTGGAGA ACAGAGTGGA GGTTGGCAAA 
GACCAGGAGT TTGTGGTTGA CACCAAAGGC GCTGGGGGGC AGGGCAAGTT GGACGTGACC 
ATTTTAAGCC CTGCGCGGCA GGTGGTCCCG TGCCTCGTGG CCTCCGTGCC AGGCAAGGAC 
TGCAGCACCG CCAAGTACAT CCCCAAGGAG GAGGGCTTGT ACGCTGTTGA CGTGAGCTAC 
GATGGGAACC CCATTCCAGG CAGCCCCTTC ACTGTGGAAG CCTCGCTGCC CCCGGACCCC 
ACAAAGGTGA AAGCCCATGG CCCCGGCCTG GAAGCCGGGT TTGTGGGAAA GCCTGCCGAG 
TTCACCATAG ACACCAAAGG AGCCGGTACC GGGGGCCTGG GCCTGACGGT TGAAGGCCCG 
TGTGAAGCCA AAATCGAGTG CTCCGACAAT GGCGACGGCA CCTGCTCAGT CTCTTACCTG 
CCGACCAAAC CTGGCGAGTA CTTTGTCAAC ATCCTCTTTG AAGAGGTCCA CATTCCCGGC 
TCTCCGTTCA AAGCCGACAT CGAGATGCCC TTCGATGCCT CCAAAGTGGT GGCCAGCGGC 
CCCGGCCTCG AGCACGGGAA AGTGGGGGAG GCCGGCGTCC TGACCGTGGA CTGTTCGGAG 
GCCGGCCGCG GGGTCCTGGG CATGGAGGCC GTGTCCGACG ATGGGGTCAG AGCGGACGTC 
CACATCCAGA ATAACAAGGA CGGCACTTAC GCCGTGACCT ATGTGCCCCT GGCTGCGGGC 
ATGTACACGC TGAGCCTGAA TTACGGCGGA GAGCAAGTGC CAGGATTCCC TGCCCGGGTC 
AAGGTGGAGC CCGCCGTGGA CACGAGCAAG ATCAAGGTCT ATGGCCCTGG CATCGAAGGG 
AAAGATGTGT TCCGGGAAGC CACGACCAGC TTCACTGTGG ACTCCCGCCC GCTGACCAAG 
GTCGGGGGAG AGCACGTCAA AGCCAGCATT GCCAACCCCT CCGGGGCCTT CACGGACTAC 
CTCCTCAAGG ACAACGCCGA CGGCACCTAC GAGGTGGAGT TCACCCCGTT TGAGAAAGGT 
TTCCACACAG TGGAAGTGAC ATATGACGAT GTGCCTGTGC CCAATAGCCC CTTCCAGGTG 
GCCGTCACCG AGGGCTGCCA TCCATCGCGA GTGCAAGCCC AAGGCCCTGG GCTGAAGGAA 
GGCTTTACCC ACAAACCCAA TCCCTTCACT GTGGTTACCA GAGGGGCAGG AATCGGGGGC 
CTGGGGATCA CGGTGGAAGG ACCCTCCGAG TCGAAGATCA GTTGCAAGGA TAACAAAGAT 
GGCAGCTGTA GCGCGGAGTA CATCCCTTTT GTGCCGGGAG ATTATGATGT CAATATTACG 
TACGGAAACG AGCATGTGCC TGGCAGCCCA TTCAGGATCC CTGTGAAAGA TGTCATAGAC 
CCCAGTAAGG TCAAGCTTGC AGGCCCTGGC CTGGGGGCAG GAGTCAGAGC TAAAGTCCCA 
CAGTCCTTCA CTGTGGACAC CAGTCAGGCT GGTCTGGCTC CCCTGGAAGT CCTGGTCCTG 
GGCCCCAGAG GCCTGGTGGA ACCGGTGACT GTGACGGACA ACGGCGATGG CACTCACACC 
GTCACCTACA CCCCCTCGCA GGAAGGCCCG TACATGCTGT CTGTCAAGTA CGCCGATGAG 
GACGTCCCCC ACAGTCCCTT TAAGGTCAAG GTCCTCCCCA CGTATGACGC TAGCAAAGTG 
ACCGCAAGCG GCCCCGGTCT CAGCTCCTAT GGGGTCCCGG CCAGCTTGCC CGTGGAGTTT 
GCAATCGATG CCAAAAAAGC AGGAATTGGG CCCCTCGCTG TCCAGATAAC GGACCAAGAA 
GGAAAGCCCA AAAAGGCCGA CGTCCACGAC AACAAAGATG GCACCTACTC CGTGACCTAC 
CTCCCAGACA AGACGGGCCG CTATCTGATC GGGGTCACGT ACGGCGGGGA AAACATCCCG 
TCTTCTCCTT ACCGCATCCG TGCAACGCCG ACTGGAGACG CCAGCAAGTG CATGGCCACA 
GGTCCTGGGA TCGCACCCAC TGTCAAAACT GGAGAGGAAG TTGGCTTTGT GGTGGATGCT 
AAGACTGCGG GGAAAGGCAA GGTGACCTGC ACGATCCTGA CCCCGGACGG CACGGAAGTG 
GAAGCCGAGG TCATCGAGAA CGAGGATGGG ACCTACGACA TCTTCTACAC GGCAGCCAAA 
CCCGGGACCT ACGTCATCTA TGTGCGCTTT GGGGGCGTGG ATATCCCCAA CAGCCCCTTC 
ACCGTCATGG CCACAGAGGA GGACGTTGCC GTAATGAAGG AGGCGCCGGT AAATGCATGT 
CCCCCCGGCT ACAGTCCTTG GGTGACGGAA GAAGCCTACG TTCCCGTCAG TGACATGAAT 
GGCCTGGGAT TTAAGCCCTT TGACCTGGTC ATTCCCTTTG CTGTACGGAA GGGAGAAGTA 
ACCGGGGAAG TGCACATGCC CTCCGGGAAG ACGGCTGCCC CCGAGATCGT CGACAACAAA 
GATGGCACCG TCACGGTTCG CTACGCGCCC ACCGAGGTCG GCCTGCACGA GATGCACATC 
AAGTACATGG GGAACCACAT CCCCGAGAGC CCGCTCCAGT TCTATGTGAA CTACCCAAAT 
AGCGGCAGCG TGTCGGCGTA CGGGCCCGGC CTGGTGTACG GCGTCGCCAA CAAGACGGCC 
ACCTTCACCA TCGTCACAGA GGATGCGGGA GAGGGGGGCC TGGACCTGGC CATCGAGGGG 
CCTTCCAAAG CTGAAATCAG CTGCATCGAC AACAAGGACG GCACCTGCAC GGTGACCTAC 
CTGCCCACAC TCCCCGGGGA CTACAGCATC CTGGTGAAGT ACAATGACAA GCACATACCC 
GGCAGCCCCT TCACCGCCAA GATCACAGAT AACAACAGGA GGTGTTCCCA AGTGAAGCTG 
GGCTCGGCTG CCGACTTCCT GCTGGACATC AGCGAGACTG ACCTGAGCAC ACTGACCGCC 
AGCATCAAAG CTCCGTCCGG GCGAGATGAG CCTTGCCTCT TGAAGAGGCT CCCAAACAAC 
CACATTGGCA TTTCTTTCAT CCCCCGGGAA GTCGGGGAAC ACCTGGTCAG CATTAAGAAA 
AATGGCAGCC ACGTGGCCAA CAGCCCGGTA TCCATCATGG TGGTTCAGTC CGAGATCGGA 
GACGCCCGGA GGGCCAAGGT CTCAGGCCGG GGCTTGAGTG AAGGGCGCAC TTTTGAAATG 
TCCGACTTCA TTGTTGACAC AAGGGATGCA GGTTACGGTG GCATATCCCT GGCGGTGGAA 
GGCCCCAGCA AGGTGGACAT CCAGACGGAA GACCTTGAAG ATGGCACCTG TAAAGTGTCC 
TACTTCCCCA CGGTGCCGGG GGTGTATATT GTATCCACCA AGTTTGCAGA TGAACACGTG 
CCAGGGAGTC CCTTCACTGT GAAAATCAGC GGAGAAGGCA GGGTCAAGGA AAGCATCACG 
CGCCCAAGTC GGGCACCGTC GGTGGCGACC GTTGGGAGCA TCTGTGACCT CAACCTGAAA 
ATCCCAGAAA TAGACAGTGC AGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCACG 
ACGGAGGCCG AGATCGTGCC CGTAGACAAG AGCTCCCACT GTGTCCGGTT CGTGCCCCAG 
GAGATGGGTG TGCACACGGT GAGCGTCAAG TACCGAGGGC AGCACGTGAC CGGAAGCCCG 
TTCCAGTTCA CAGTTGGGCC CCTCGGGGAA GGGGGTGCCC AGAAAGTCCG CGCCGGGGGC 
CCCGGCCTCG AGAGAGGAGA AGCGGGAGTG CCAGCTGAAT TTAGCATTTG GACCAGAGAA 
GCAGGGGCCG GGGGTCTCTC GATTGCTGTC GAAGGACCGA GTAAGGCAGA AATTGCCTTT 
GACGACCACA AAAACGGGTC GTGTGGCGTG TCTTACATTG CTCAAGAACC GGGTAACTAT 
GAGGTGTCGA TCAAGTTCAA CGACGAGCAC ATCCCAGAGA GCCCCTACTT GGTGCCCATC 
ATCGCTCCTT CCGACGACGC CCGCAGACTC ACTGTTATGA GCCTTCAGGA ATCTGGATTA 
AAAGTTAACC AGCCAGCATC CTTTGCGATA AGGCTGAATG GGGCCAAAGG CAAGATCGAT 
GCAAAAGTCC ACAGCCCCTC GGGTGCCGTC GAGGAGTGCC ACGTGTCCGA GCTGGAGCCA 
GATAAGTACG CCGTGCGCTT CATTCCCCAC GAGAACGGCA TTCACACGAT CGACGTCAAG 
TTCAATGGCA GCCACGTGGT TGGAAGCCCT TTTAAAGTTC GAGTCGGGGA TCCAGGACAA 
GCGGGAAATC CAGCTCTCGT CTCTGCCTAT GGAGCTGGCC TGGAAGGGGG GACCACAGGC 
GTCCAATCGG AGTTTTTCAT CAATACCACC AGAGCTGGCC CCGGGACACT GTCTGTTACA 
ATTGAGGGAC CGTCCAAGGT CAAAATGGAC TGCCAGGAGA CTCCAGAAGG TTACAAAGTC 
ATGTATACAC CGATGGCTCC TGGGAATTAT CTGATCGGTG TGAAATACGG CGGACCCAAC 
CACATCGTTG GCAGCCCTTT CAAGGCCAAG GTGACAGGTC AACGCCTCGT CAGCCCGTGC 
TCTGCCAACG AGACATCTTC CATCCTGGTT GAGTCGGTGA CCAGATCCTC CACAGAAACC 
TGCTACAGCG CCGTCCCCAA AGCTTCTTCA GACGCCAGCA AAGTGACTTC AAAGGGAGCA 
GGGCTCGTGA AAGCTTTCGT GGGCCAGAAG AGCAGCTTCC TGGTCGACTG CAGCAAGGCG 
GGTTCCAATA TGTTACTGAT TGGTGTCCAT GGTCCCACCA CGCCTTGTGA AGAAGTGTCC 
ATGAAACATG TAGGAAACCA GCAGTATAAT GTGACTTATG TTGTCAAGGA GAAGGGGGAT 
TATGTTTTGG CTGTCAAGTG GGGTGAGGAG CACATTCCGG GAAGTCCCTT CCATATCACA 
GTACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FLNB cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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