CHD4 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHD4
Entrez Gene ID	100921881
Full Name	chromodomain helicase DNA binding protein 4
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012550712.1	XP_012406166.1	LOW QUALITY PROTEIN: chromodomain-helicase-DNA-binding protein 4
XM_023504578.1	XP_023360346.1	LOW QUALITY PROTEIN: chromodomain-helicase-DNA-binding protein 4
XM_031938277.1	XP_031794137.1	chromodomain-helicase-DNA-binding protein 4 isoform X1
XM_031938280.1	XP_031794140.1	chromodomain-helicase-DNA-binding protein 4 isoform X4
XM_031938281.1	XP_031794141.1	chromodomain-helicase-DNA-binding protein 4 isoform X5
XM_031938282.1	XP_031794142.1	chromodomain-helicase-DNA-binding protein 4 isoform X6
XM_031938278.1	XP_031794138.1	chromodomain-helicase-DNA-binding protein 4 isoform X2
XM_031938279.1	XP_031794139.1	chromodomain-helicase-DNA-binding protein 4 isoform X3

Pathways from BioSystems
Gene Ontology

The following CHD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa133447	XM_012550712.1 Latest version!	Sarcophilus harrisii chromodomain-helicase-DNA-binding protein 4 (LOC100921881), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa156102	XM_023504578.1 Latest version!	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa236821	XM_031938277.1 Latest version!	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa236822	XM_031938278.1 Latest version!	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa236823	XM_031938279.1 Latest version!	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa236824	XM_031938280.1 Latest version!	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa236825	XM_031938281.1 Latest version!	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa236826	XM_031938282.1 Latest version!	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHD4 ( XM_012550712.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406166.1 Sarcophilus harrisii chromodomain-helicase-DNA-binding protein 4 (LOC100921881), mRNA.

Price & Availability↑

CloneID	OSa133447
Clone ID Related Accession (Same CDS sequence)	XM_012550712.1
Accession Version	XM_012550712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: chromodomain-helicase-DNA-binding protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843622.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGGTCATTC AAAAGGCCCT 
TTCCTAGTGA GTGCCCCACT TTCTACAATC ATCAACTGGG AGCGAGAGTT TGAGATGTGG 
GCTCCAGACA TGTATGTGGT AACCTATGTT GGAGACAAGG ATAGTCGTGC TATTATTCGT 
GAAAACGAGT TCTCTTTTGA AGACAATGCT ATCCGTGGTG GCAAGAAGGC TTCCAGAATG 
AAGAAAGAAG CATCTGTGAA GTTTCATGTT TTGCTGACTT CCTATGAACT GATTACCATT 
GACATGGCCA TTCTGGGCTC CATTGATTGG GCCTGCCTAA TTGTGGATGA AGCTCATAGG 
CTTAAGAACA ATCAGTCTAA GTTTTTCAGG GTACTCAATG GCTATTCCCT CCAGCACAAG 
TTACTACTAA CAGGGACTCC TCTACAGAAT AACTTGGAGG AGCTTTTCCA CCTGCTTAAT 
TTCCTCACTC CAGAAAGATT CCACAACCTG GAGGGCTTCC TAGAAGAATT TGCTGACATT 
GCCAAGGAAG ACCAGATCAA AAAATTGCAT GACATGTTGG GGCCTCATAT GCTTCGACGG 
CTTAAAGCTG ATGTCTTCAA GAACATGCCT TCTAAGACAG AGCTGATTGA GCCCAATGCA 
GAAGAAATAC TACAAATGAC CAAGATGTTG GATCTGTTAG AGGATTTTTT GGAACACGAA 
GGTTATAAAT ATGAGCGAAT CGATGGGGAA ATTACTGGGA ACACGCGTCA GGAAGCCATT 
GATCGTTTTA ACACACCTGG TGCTCAGCAG TTTTGCTTTC TTCTTTCTAC TCGAGCTGGG 
GGCCTGGGAA TCAGTCTAGC TACTGCAGAT ACTGTTATCA TCTATGACTC TGATTGGAAC 
CCCCATAATG ACATCCAGGC TTTTAGCAGA GCCCATCGAA TTGGTCAGAA TAAGAAAGTG 
ATGATCTACC GTTTTGTGAC CCACGCTTCA GTGGAGGAGC ACATCACACA GGAAAAGGAA 
GGTATCCAGT GGGAAGCAAA GGAGGACAAC TCTGAGGGTG AAGAAATCCT AGAAGAAGTT 
GGTGGTGACC CTGAGGAGGA GGATGATCAT CACATGGAGT TTTGCCGTGT CTGCAAGGAT 
GGTGGGGAAC TACTCTGCTG TGATACCTGC CCCTCTTCCT ACCATATCCA CTGCTTGAAC 
CCCCCACTCC CAGAGATCCC CAACGGCGAA TGGCTTTGTC CTCGTTGCAC ATGCCCTTCA 
CTGAAAGGTA AAGTTCAGAA GATCCTGATT TGGAAATGGG GGCAGCCACC ATCCCCTACA 
CCAGTGCCCC GACCCCCTGA TGCTGACCCC AATACCCCTT CTCCTAAGCC TTTAGAGGGG 
CGACCTGAGC GGCAATTCTT TGTGAAATGG CAAGGCATGT CCTACTGGCA CTGTTCTTGG 
GTGTCAGAAC TACAGCTGGA GCTTCACTGC CAAGTGATGT TCCGTAACTA CCAGCGTAAG 
AATGATATGG ATGAGCCACC ATCTGGTGAC TTTGGTGGGG AGGAAGAAAA AAGCCGTAAA 
CGAAAGAACA AGGACCCCAA GTTTGCAGAA ATGGAAGAAC GTTTCTACCG CTATGGGATT 
AAACCTGAGT GGATGATGAT CCACCGTATC CTCAACCACA GTGTAGATAA GAAGGGTCAT 
GTCCACTATT TGATCAAATG GAGGGACCTG CCCTATGATC AGGCATCCTG GGAGAGTGAA 
GATGTAGAGG TGCAAGACTA TGACCTATTC AAGCAGAGCT ATTGGAACCA TAGGGAGCTA 
ATGCGGGGTG AGGAAGGACG ACCTGGCAAA AAGCTCAAGA AGGTTAAGCT TAGGAAGTTG 
GAGAGGCCTC CTGAAACTCC TACAGTTGAT CCAACAGTGA AATATGAGCG GCAACCAGAG 
TACTTAGATG CCACTGGTGG GACTCTACAT CCCTATCAAA TGGAGGGATT GAACTGGTTG 
CGCTTCTCCT GGGCCCAGGG CACTGACACT ATCTTGGCAG ATGAAATGGG CTTGGGCAAG 
ACGGTTCAGA CCGCTGTCTT CCTCTATTCT TTGTACAAGG AGGGTCATTC AAAAGGCCCT 
TTCCTAGTGA GTGCCCCACT TTCTACAATC ATCAACTGGG AGCGAGAGTT TGAGATGTGG 
GCTCCAGACA TGTATGTGGT AACCTATGTT GGAGACAAGG ATAGTCATGC TATTATTCGT 
GAAAAGGAGT TCTCTTTTGA AGACAATGCT ATCCATGGTG GCAAGAAGGC TTCCAGAATG 
AAGAAAGAAG CATCTGTGAA GTTTCATGTT TTGCTGACTT CCTATGAACT GATTACCATT 
GACATGGCCA TTCTGGGCTC CATTGATTGG GCCTGCCTAA TTGTGGATGA AGCTCATAGG 
CTTTTCAGGG TACTCAATGG CTATTCCCTC CAGCACAAGT TACTACTAAC AGGGACTCCT 
CTACAGAATA ACTTGGAAGA GCTTTTCCAC CTGCTTAATT TCCTCACTCC AGAAAGATTC 
CACAACCTGG AGGGCTTCCT AGAAGAATTT GCTGACATTG CCAAGGAAGA CCAGATCAAA 
AAATTGCATG ACATGTTGGG GCCTCATATG CTTCGACGGC TTAAAGCTGA TGTCTTCAAG 
AACATGCCTT CTAAGACAGA GCTGNNATTG AGCCCAATGC AGAAGAAATA CTACAAGTAC 
ATTCTAACCC GAAACTTTGA GGCACTCAAT GCTCGGGGTG GTGGCAATCA GGTTTCCCTA 
CTTAATGTGG TGATGGATTT AAAAAAGTGC TGTAACCACC CTTACCTCTT CCCTGTGGCT 
GCCATGGAAG CACCAAAGAT GCCCAATGGG ATGTATGATG GCAGTGCTCT AATTCAAGCA 
TCAGGGAAGC TACTTTTATT GCAAAAAATG CTCAAGAAAC TCAAAGAGGG TGGTCACCGT 
GTGCTCATTT TCTCACAGAT GACCAAGATG TTGGATCTGT TAGAGGATTT TTTGGAACAC 
GAAGGTTATA AATATGAGCG AATCGATGGG GAAATTACTG GGAACACGCG TCAGGAAGCC 
ATTGATCGTT TTAACACACC TGGTGCTCAG CAGTTTTGCT TTCTTCTTTC TACTCGAGCT 
GGGGGCCTGG GAATCAGTCT AGCTACTGCA GATACTGTTA TCATCTATGA CTCTGATTGG 
AACCCCCATA ATGACATCCA GGCTTTTAGC AGAGCCCATC GAATTGGTCA GAATAAGAAA 
GTGATGATCT ACCGTTTTGT GACCCACGCT TCAGTGGAGG AGCACATCAC ACAGGTGGCG 
AAGAAGAAGA TGATGCTTAC ACATCTTGTA GTAAGGCCTG GCCTGGGCTC TAAGACTGGC 
TCCATGTCCA AGCAGGAGCT TGATGATATC CTCAAGTTTG GCACTGAGGA ATTATTCAAA 
GATGAAGCCA CTGATGGAGG AGGAGACAAC AAGGAAGGAG AAGACAGTAG TGTTATTCAC 
TATGATGACA AGGCCATAGA GCGACTGCTG GATCGAAATC AGGATGAAAC AGAAGACACA 
GAACTTCAAG GCATGAATGA ATATTTGAGT TCCTTCAAAG TGGCCCAGTA TGTAGTACAG 
GAAGAAGAAA TGGGGGAGGA AGAAGAAGTA GAAAGAGAGA TCATCAAGCA GGAAGAAAGT 
GTGGATCCTG ACTACTGGGA GAAGCTGCTG CGACACCATT ATGAGCAGCA GCAAGAGGAT 
CTGGCCCGAA ACCTAGGCAA AGGGAAGCGA ATTCGTAAGC AGGTCAACTA CAACGATGGC 
TCCCAGGAGG ATCGAGATTG GCAGGATGAC CAGTCAGACA ATCAGTCCGA CTACTCAGTG 
GCCTCAGAGG AAGGCGATGA GGACTTTGAT GAGCGTTCAG AAGCTCCCAG ACGGCCTAGC 
CGCAAGGGCC TGCGAAATGA TAAGGACAAA CCATTGCCTC CATTGTTGGC TCGTGTTGGT 
GGAAATATTG AGGTACTTGG CTTCAATGCC CGCCAGCGGA AGGCTTTCCT CAATGCCATT 
ATGCGTTATG GGATGCCTCC TCAGGATGCC TTTACTACCC AATGGCTTGT GCGGGACCTG 
AGGGGCAAGT CAGAGAAAGA GTTCAAGGCA TATGTGTCTC TCTTTATGCG TCACCTTTGT 
GAACCAGGAG CAGATGGGGC TGAGACCTTT GCTGATGGTG TACCCCGTGA GGGCTTATCC 
CGGCAGCATG TTCTTACTCG TATTGGTGTT ATGTCACTTA TCCGAAAAAA GGTTCAGGAA 
TTTGAGCATG TCAATGGGCG CTGGAGCATG CCTGAGCTGG CTGAGGTAGA AGAGAACAAG 
AAGATGTCAC AGCCAGGATC ACCTTCACCT AAAACTCCCA CACCTTCCAC TCCAGGAGAC 
ACACAACCCA ACACTCCTGC ACCTGTGCCA CCCCCTGATG ATGGGGTGAA AGCAGAGGAG 
AATAATAACA AAGAAGAAGA GAACACAGAA GGAGAGAGAG AGGTGAAACC TGCAGCTCCT 
GAGGCTTCTG TGGAGGCTAC ACAGCCCTCT GCATCTAACC CAGAAGAGGA AAAAGTTCTG 
ACTGAGCCTC TGGAAGGAGA AGAGAAGGTG GATAAGCCAG AAGTCAAAGA GAAGACAGAG 
GAGACCATGG AGACAGAAAC CAAAGGTGTT GCTGAAACGG AAAAGGTAGA GGAGAAAGCA 
ACAGTGGATC TGACCCCTAT TGTGGTAGAA GACAAAGAGG AGAAGAAAGA AGAAGAAGAG 
AAAAAAGAAG TGATGCTTCA GAATGGGGAG ACTCCCAAGG ACCTGGGTGA TGAGAAGCAG 
AAGAAGAACA TCAAGCAGCG TTTTATGTTC AACATTGCAG ATGGTGGCTT CACTGAGCTG 
CATTCCCTTT GGCAGAATGA GGAACGGGCA GCCACAGTTA CAAAGAAGAC CTATGAGATC 
TGGCATCGGC GGCATGACTA CTGGCTGCTG GCTGGCATTA TTAACCATGG CTATGCTCGG 
TGGCAGGACA TTCAGAATGA CCCGCGCTAT GCCATTCTTA ATGAACCCTT CAAAGGTGAA 
ATGAATCGTG GTAACTTCTT GGAGATAAAA AACAAATTCC TGGCTAGAAG ATTTAAGGTG 
AGCAAGGAGA AGAGGAATAT AATCTTTGGT TTTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406166.1
CDS	88..5586
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERVLLCRQLGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIEPNAEEILQMTKMLDLLEDFLEHEGYKYERIDGEITGNTRQEAIDRFNTPGAQQFCFLLSTRAGGLGISLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTHASVEEHITQEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSHAIIREKEFSFEDNAIHGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELnullLSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIQASGKLLLLQKMLKKLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGEITGNTRQEAIDRFNTPGAQQFCFLLSTRAGGLGISLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTHASVEEHITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVQEEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKVSKEKRNIIFGFL

Target ORF information:

RefSeq Version	XM_012550712.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain-helicase-DNA-binding protein 4 (LOC100921881), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550712.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGGTCATTC AAAAGGCCCT 
TTCCTAGTGA GTGCCCCACT TTCTACAATC ATCAACTGGG AGCGAGAGTT TGAGATGTGG 
GCTCCAGACA TGTATGTGGT AACCTATGTT GGAGACAAGG ATAGTCGTGC TATTATTCGT 
GAAAACGAGT TCTCTTTTGA AGACAATGCT ATCCGTGGTG GCAAGAAGGC TTCCAGAATG 
AAGAAAGAAG CATCTGTGAA GTTTCATGTT TTGCTGACTT CCTATGAACT GATTACCATT 
GACATGGCCA TTCTGGGCTC CATTGATTGG GCCTGCCTAA TTGTGGATGA AGCTCATAGG 
CTTAAGAACA ATCAGTCTAA GTTTTTCAGG GTACTCAATG GCTATTCCCT CCAGCACAAG 
TTACTACTAA CAGGGACTCC TCTACAGAAT AACTTGGAGG AGCTTTTCCA CCTGCTTAAT 
TTCCTCACTC CAGAAAGATT CCACAACCTG GAGGGCTTCC TAGAAGAATT TGCTGACATT 
GCCAAGGAAG ACCAGATCAA AAAATTGCAT GACATGTTGG GGCCTCATAT GCTTCGACGG 
CTTAAAGCTG ATGTCTTCAA GAACATGCCT TCTAAGACAG AGCTGATTGA GCCCAATGCA 
GAAGAAATAC TACAAATGAC CAAGATGTTG GATCTGTTAG AGGATTTTTT GGAACACGAA 
GGTTATAAAT ATGAGCGAAT CGATGGGGAA ATTACTGGGA ACACGCGTCA GGAAGCCATT 
GATCGTTTTA ACACACCTGG TGCTCAGCAG TTTTGCTTTC TTCTTTCTAC TCGAGCTGGG 
GGCCTGGGAA TCAGTCTAGC TACTGCAGAT ACTGTTATCA TCTATGACTC TGATTGGAAC 
CCCCATAATG ACATCCAGGC TTTTAGCAGA GCCCATCGAA TTGGTCAGAA TAAGAAAGTG 
ATGATCTACC GTTTTGTGAC CCACGCTTCA GTGGAGGAGC ACATCACACA GGAAAAGGAA 
GGTATCCAGT GGGAAGCAAA GGAGGACAAC TCTGAGGGTG AAGAAATCCT AGAAGAAGTT 
GGTGGTGACC CTGAGGAGGA GGATGATCAT CACATGGAGT TTTGCCGTGT CTGCAAGGAT 
GGTGGGGAAC TACTCTGCTG TGATACCTGC CCCTCTTCCT ACCATATCCA CTGCTTGAAC 
CCCCCACTCC CAGAGATCCC CAACGGCGAA TGGCTTTGTC CTCGTTGCAC ATGCCCTTCA 
CTGAAAGGTA AAGTTCAGAA GATCCTGATT TGGAAATGGG GGCAGCCACC ATCCCCTACA 
CCAGTGCCCC GACCCCCTGA TGCTGACCCC AATACCCCTT CTCCTAAGCC TTTAGAGGGG 
CGACCTGAGC GGCAATTCTT TGTGAAATGG CAAGGCATGT CCTACTGGCA CTGTTCTTGG 
GTGTCAGAAC TACAGCTGGA GCTTCACTGC CAAGTGATGT TCCGTAACTA CCAGCGTAAG 
AATGATATGG ATGAGCCACC ATCTGGTGAC TTTGGTGGGG AGGAAGAAAA AAGCCGTAAA 
CGAAAGAACA AGGACCCCAA GTTTGCAGAA ATGGAAGAAC GTTTCTACCG CTATGGGATT 
AAACCTGAGT GGATGATGAT CCACCGTATC CTCAACCACA GTGTAGATAA GAAGGGTCAT 
GTCCACTATT TGATCAAATG GAGGGACCTG CCCTATGATC AGGCATCCTG GGAGAGTGAA 
GATGTAGAGG TGCAAGACTA TGACCTATTC AAGCAGAGCT ATTGGAACCA TAGGGAGCTA 
ATGCGGGGTG AGGAAGGACG ACCTGGCAAA AAGCTCAAGA AGGTTAAGCT TAGGAAGTTG 
GAGAGGCCTC CTGAAACTCC TACAGTTGAT CCAACAGTGA AATATGAGCG GCAACCAGAG 
TACTTAGATG CCACTGGTGG GACTCTACAT CCCTATCAAA TGGAGGGATT GAACTGGTTG 
CGCTTCTCCT GGGCCCAGGG CACTGACACT ATCTTGGCAG ATGAAATGGG CTTGGGCAAG 
ACGGTTCAGA CCGCTGTCTT CCTCTATTCT TTGTACAAGG AGGGTCATTC AAAAGGCCCT 
TTCCTAGTGA GTGCCCCACT TTCTACAATC ATCAACTGGG AGCGAGAGTT TGAGATGTGG 
GCTCCAGACA TGTATGTGGT AACCTATGTT GGAGACAAGG ATAGTCATGC TATTATTCGT 
GAAAAGGAGT TCTCTTTTGA AGACAATGCT ATCCATGGTG GCAAGAAGGC TTCCAGAATG 
AAGAAAGAAG CATCTGTGAA GTTTCATGTT TTGCTGACTT CCTATGAACT GATTACCATT 
GACATGGCCA TTCTGGGCTC CATTGATTGG GCCTGCCTAA TTGTGGATGA AGCTCATAGG 
CTTTTCAGGG TACTCAATGG CTATTCCCTC CAGCACAAGT TACTACTAAC AGGGACTCCT 
CTACAGAATA ACTTGGAAGA GCTTTTCCAC CTGCTTAATT TCCTCACTCC AGAAAGATTC 
CACAACCTGG AGGGCTTCCT AGAAGAATTT GCTGACATTG CCAAGGAAGA CCAGATCAAA 
AAATTGCATG ACATGTTGGG GCCTCATATG CTTCGACGGC TTAAAGCTGA TGTCTTCAAG 
AACATGCCTT CTAAGACAGA GCTGNNATTG AGCCCAATGC AGAAGAAATA CTACAAGTAC 
ATTCTAACCC GAAACTTTGA GGCACTCAAT GCTCGGGGTG GTGGCAATCA GGTTTCCCTA 
CTTAATGTGG TGATGGATTT AAAAAAGTGC TGTAACCACC CTTACCTCTT CCCTGTGGCT 
GCCATGGAAG CACCAAAGAT GCCCAATGGG ATGTATGATG GCAGTGCTCT AATTCAAGCA 
TCAGGGAAGC TACTTTTATT GCAAAAAATG CTCAAGAAAC TCAAAGAGGG TGGTCACCGT 
GTGCTCATTT TCTCACAGAT GACCAAGATG TTGGATCTGT TAGAGGATTT TTTGGAACAC 
GAAGGTTATA AATATGAGCG AATCGATGGG GAAATTACTG GGAACACGCG TCAGGAAGCC 
ATTGATCGTT TTAACACACC TGGTGCTCAG CAGTTTTGCT TTCTTCTTTC TACTCGAGCT 
GGGGGCCTGG GAATCAGTCT AGCTACTGCA GATACTGTTA TCATCTATGA CTCTGATTGG 
AACCCCCATA ATGACATCCA GGCTTTTAGC AGAGCCCATC GAATTGGTCA GAATAAGAAA 
GTGATGATCT ACCGTTTTGT GACCCACGCT TCAGTGGAGG AGCACATCAC ACAGGTGGCG 
AAGAAGAAGA TGATGCTTAC ACATCTTGTA GTAAGGCCTG GCCTGGGCTC TAAGACTGGC 
TCCATGTCCA AGCAGGAGCT TGATGATATC CTCAAGTTTG GCACTGAGGA ATTATTCAAA 
GATGAAGCCA CTGATGGAGG AGGAGACAAC AAGGAAGGAG AAGACAGTAG TGTTATTCAC 
TATGATGACA AGGCCATAGA GCGACTGCTG GATCGAAATC AGGATGAAAC AGAAGACACA 
GAACTTCAAG GCATGAATGA ATATTTGAGT TCCTTCAAAG TGGCCCAGTA TGTAGTACAG 
GAAGAAGAAA TGGGGGAGGA AGAAGAAGTA GAAAGAGAGA TCATCAAGCA GGAAGAAAGT 
GTGGATCCTG ACTACTGGGA GAAGCTGCTG CGACACCATT ATGAGCAGCA GCAAGAGGAT 
CTGGCCCGAA ACCTAGGCAA AGGGAAGCGA ATTCGTAAGC AGGTCAACTA CAACGATGGC 
TCCCAGGAGG ATCGAGATTG GCAGGATGAC CAGTCAGACA ATCAGTCCGA CTACTCAGTG 
GCCTCAGAGG AAGGCGATGA GGACTTTGAT GAGCGTTCAG AAGCTCCCAG ACGGCCTAGC 
CGCAAGGGCC TGCGAAATGA TAAGGACAAA CCATTGCCTC CATTGTTGGC TCGTGTTGGT 
GGAAATATTG AGGTACTTGG CTTCAATGCC CGCCAGCGGA AGGCTTTCCT CAATGCCATT 
ATGCGTTATG GGATGCCTCC TCAGGATGCC TTTACTACCC AATGGCTTGT GCGGGACCTG 
AGGGGCAAGT CAGAGAAAGA GTTCAAGGCA TATGTGTCTC TCTTTATGCG TCACCTTTGT 
GAACCAGGAG CAGATGGGGC TGAGACCTTT GCTGATGGTG TACCCCGTGA GGGCTTATCC 
CGGCAGCATG TTCTTACTCG TATTGGTGTT ATGTCACTTA TCCGAAAAAA GGTTCAGGAA 
TTTGAGCATG TCAATGGGCG CTGGAGCATG CCTGAGCTGG CTGAGGTAGA AGAGAACAAG 
AAGATGTCAC AGCCAGGATC ACCTTCACCT AAAACTCCCA CACCTTCCAC TCCAGGAGAC 
ACACAACCCA ACACTCCTGC ACCTGTGCCA CCCCCTGATG ATGGGGTGAA AGCAGAGGAG 
AATAATAACA AAGAAGAAGA GAACACAGAA GGAGAGAGAG AGGTGAAACC TGCAGCTCCT 
GAGGCTTCTG TGGAGGCTAC ACAGCCCTCT GCATCTAACC CAGAAGAGGA AAAAGTTCTG 
ACTGAGCCTC TGGAAGGAGA AGAGAAGGTG GATAAGCCAG AAGTCAAAGA GAAGACAGAG 
GAGACCATGG AGACAGAAAC CAAAGGTGTT GCTGAAACGG AAAAGGTAGA GGAGAAAGCA 
ACAGTGGATC TGACCCCTAT TGTGGTAGAA GACAAAGAGG AGAAGAAAGA AGAAGAAGAG 
AAAAAAGAAG TGATGCTTCA GAATGGGGAG ACTCCCAAGG ACCTGGGTGA TGAGAAGCAG 
AAGAAGAACA TCAAGCAGCG TTTTATGTTC AACATTGCAG ATGGTGGCTT CACTGAGCTG 
CATTCCCTTT GGCAGAATGA GGAACGGGCA GCCACAGTTA CAAAGAAGAC CTATGAGATC 
TGGCATCGGC GGCATGACTA CTGGCTGCTG GCTGGCATTA TTAACCATGG CTATGCTCGG 
TGGCAGGACA TTCAGAATGA CCCGCGCTAT GCCATTCTTA ATGAACCCTT CAAAGGTGAA 
ATGAATCGTG GTAACTTCTT GGAGATAAAA AACAAATTCC TGGCTAGAAG ATTTAAGGTG 
AGCAAGGAGA AGAGGAATAT AATCTTTGGT TTTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_023504578.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023360346.1 Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), mRNA.

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CloneID	OSa156102
Clone ID Related Accession (Same CDS sequence)	XM_023504578.1
Accession Version	XM_023504578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: chromodomain-helicase-DNA-binding protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843622.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGGTCA TTCAAAAGGC CCTTTCCTAG TGAGTGCCCC ACTTTCTACA 
ATCATCAACT GGGAGCGAGA GTTTGAGATG TGGGCTCCAG ACATGTATGT GGTAACCTAT 
GTTGGAGACA AGGATAGTCG TGCTATTATT CGTGAAAACG AGTTCTCTTT TGAAGACAAT 
GCTATCCGTG GTGGCAAGAA GGCTTCCAGA ATGAAGAAAG AAGCATCTGT GAAGTTTCAT 
GTTTTGCTGA CTTCCTATGA ACTGATTACC ATTGACATGG CCATTCTGGG CTCCATTGAT 
TGGGCCTGCC TAATTGTGGA TGAAGCTCAT AGGCTTAAGA ACAATCAGTC TAAGTTTTTC 
AGGGTACTCA ATGGCTATTC CCTCCAGCAC AAGTTACTAC TAACAGGGAC TCCTCTACAG 
AATAACTTGG AGGAGCTTTT CCACCTGCTT AATTTCCTCA CTCCAGAAAG ATTCCACAAC 
CTGGAGGGCT TCCTAGAAGA ATTTGCTGAC ATTGCCAAGG AAGACCAGAT CAAAAAATTG 
CATGACATGT TGGGGCCTCA TATGCTTCGA CGGCTTAAAG CTGATGTCTT CAAGAACATG 
CCTTCTAAGA CAGAGCTGAT TGAGCCCAAT GCAGAAGAAA TACTACAAAT GACCAAGATG 
TTGGATCTGT TAGAGGATTT TTTGGAACAC GAAGGTTATA AATATGAGCG AATCGATGGG 
GAAATTACTG GGAACACGCG TCAGGAAGCC ATTGATCGTT TTAACACACC TGGTGCTCAG 
CAGTTTTGCT TTCTTCTTTC TACTCGAGCT GGGGGCCTGG GAATCAGTCT AGCTACTGCA 
GATACTGTTA TCATCTATGA CTCTGATTGG AACCCCCATA ATGACATCCA GGCTTTTAGC 
AGAGCCCATC GAATTGGTCA GAATAAGAAA GTGATGATCT ACCGTTTTGT GACCCACGCT 
TCAGTGGAGG AGCACATCAC ACAGGAAAAG GAAGGTATCC AGTGGGAAGC AAAGGAGGAC 
AACTCTGAGG GTGAAGAAAT CCTAGAAGAA GTTGGTGGTG ACCCTGAGGA GGAGGATGAT 
CATCACATGG AGTTTTGCCG TGTCTGCAAG GATGGTGGGG AACTACTCTG CTGTGATACC 
TGCCCCTCTT CCTACCATAT CCACTGCTTG AACCCCCCAC TCCCAGAGAT CCCCAACGGC 
GAATGGCTTT GTCCTCGTTG CACATGCCCT TCACTGAAAG GTAAAGTTCA GAAGATCCTG 
ATTTGGAAAT GGGGGCAGCC ACCATCCCCT ACACCAGTGC CCCGACCCCC TGATGCTGAC 
CCCAATACCC CTTCTCCTAA GCCTTTAGAG GGGCGACCTG AGCGGCAATT CTTTGTGAAA 
TGGCAAGGCA TGTCCTACTG GCACTGTTCT TGGGTGTCAG AACTACAGCT GGAGCTTCAC 
TGCCAAGTGA TGTTCCGTAA CTACCAGCGT AAGAATGATA TGGATGAGCC ACCATCTGGT 
GACTTTGGTG GGGAGGAAGA AAAAAGCCGT AAACGAAAGA ACAAGGACCC CAAGTTTGCA 
GAAATGGAAG AACGTTTCTA CCGCTATGGG ATTAAACCTG AGTGGATGAT GATCCACCGT 
ATCCTCAACC ACAGTGTAGA TAAGAAGGGT CATGTCCACT ATTTGATCAA ATGGAGGGAC 
CTGCCCTATG ATCAGGCATC CTGGGAGAGT GAAGATGTAG AGGTGCAAGA CTATGACCTA 
TTCAAGCAGA GCTATTGGAA CCATAGGGAG CTAATGCGGG GTGAGGAAGG ACGACCTGGC 
AAAAAGCTCA AGAAGGTTAA GCTTAGGAAG TTGGAGAGGC CTCCTGAAAC TCCTACAGTT 
GATCCAACAG TGAAATATGA GCGGCAACCA GAGTACTTAG ATGCCACTGG TGGGACTCTA 
CATCCCTATC AAATGGAGGG ATTGAACTGG TTGCGCTTCT CCTGGGCCCA GGGCACTGAC 
ACTATCTTGG CAGATGAAAT GGGCTTGGGC AAGACGGTTC AGACCGCTGT CTTCCTCTAT 
TCTTTGTACA AGGAGGGTCA TTCAAAAGGC CCTTTCCTAG TGAGTGCCCC ACTTTCTACA 
ATCATCAACT GGGAGCGAGA GTTTGAGATG TGGGCTCCAG ACATGTATGT GGTAACCTAT 
GTTGGAGACA AGGATAGTCA TGCTATTATT CGTGAAAAGG AGTTCTCTTT TGAAGACAAT 
GCTATCCATG GTGGCAAGAA GGCTTCCAGA ATGAAGAAAG AAGCATCTGT GAAGTTTCAT 
GTTTTGCTGA CTTCCTATGA ACTGATTACC ATTGACATGG CCATTCTGGG CTCCATTGAT 
TGGGCCTGCC TAATTGTGGA TGAAGCTCAT AGGCTTTTCA GGGTACTCAA TGGCTATTCC 
CTCCAGCACA AGTTACTACT AACAGGGACT CCTCTACAGA ATAACTTGGA AGAGCTTTTC 
CACCTGCTTA ATTTCCTCAC TCCAGAAAGA TTCCACAACC TGGAGGGCTT CCTAGAAGAA 
TTTGCTGACA TTGCCAAGGA AGACCAGATC AAAAAATTGC ATGACATGTT GGGGCCTCAT 
ATGCTTCGAC GGCTTAAAGC TGATGTCTTC AAGAACATGC CTTCTAAGAC AGAGCTGNNA 
TTGAGCCCAA TGCAGAAGAA ATACTACAAG TACATTCTAA CCCGAAACTT TGAGGCACTC 
AATGCTCGGG GTGGTGGCAA TCAGGTTTCC CTACTTAATG TGGTGATGGA TTTAAAAAAG 
TGCTGTAACC ACCCTTACCT CTTCCCTGTG GCTGCCATGG AAGCACCAAA GATGCCCAAT 
GGGATGTATG ATGGCAGTGC TCTAATTCAA GCATCAGGGA AGCTACTTTT ATTGCAAAAA 
ATGCTCAAGA AACTCAAAGA GGGTGGTCAC CGTGTGCTCA TTTTCTCACA GATGACCAAG 
ATGTTGGATC TGTTAGAGGA TTTTTTGGAA CACGAAGGTT ATAAATATGA GCGAATCGAT 
GGGGAAATTA CTGGGAACAC GCGTCAGGAA GCCATTGATC GTTTTAACAC ACCTGGTGCT 
CAGCAGTTTT GCTTTCTTCT TTCTACTCGA GCTGGGGGCC TGGGAATCAG TCTAGCTACT 
GCAGATACTG TTATCATCTA TGACTCTGAT TGGAACCCCC ATAATGACAT CCAGGCTTTT 
AGCAGAGCCC ATCGAATTGG TCAGAATAAG AAAGTGATGA TCTACCGTTT TGTGACCCAC 
GCTTCAGTGG AGGAGCACAT CACACAGGTG GCGAAGAAGA AGATGATGCT TACACATCTT 
GTAGTAAGGC CTGGCCTGGG CTCTAAGACT GGCTCCATGT CCAAGCAGGA GCTTGATGAT 
ATCCTCAAGT TTGGCACTGA GGAATTATTC AAAGATGAAG CCACTGATGG AGGAGGAGAC 
AACAAGGAAG GAGAAGACAG TAGTGTTATT CACTATGATG ACAAGGCCAT AGAGCGACTG 
CTGGATCGAA ATCAGGATGA AACAGAAGAC ACAGAACTTC AAGGCATGAA TGAATATTTG 
AGTTCCTTCA AAGTGGCCCA GTATGTAGTA CAGGAAGAAG AAATGGGGGA GGAAGAAGAA 
GTAGAAAGAG AGATCATCAA GCAGGAAGAA AGTGTGGATC CTGACTACTG GGAGAAGCTG 
CTGCGACACC ATTATGAGCA GCAGCAAGAG GATCTGGCCC GAAACCTAGG CAAAGGGAAG 
CGAATTCGTA AGCAGGTCAA CTACAACGAT GGCTCCCAGG AGGATCGAGA TTGGCAGGAT 
GACCAGTCAG ACAATCAGTC CGACTACTCA GTGGCCTCAG AGGAAGGCGA TGAGGACTTT 
GATGAGCGTT CAGAAGCTCC CAGACGGCCT AGCCGCAAGG GCCTGCGAAA TGATAAGGAC 
AAACCATTGC CTCCATTGTT GGCTCGTGTT GGTGGAAATA TTGAGGTACT TGGCTTCAAT 
GCCCGCCAGC GGAAGGCTTT CCTCAATGCC ATTATGCGTT ATGGGATGCC TCCTCAGGAT 
GCCTTTACTA CCCAATGGCT TGTGCGGGAC CTGAGGGGCA AGTCAGAGAA AGAGTTCAAG 
GCATATGTGT CTCTCTTTAT GCGTCACCTT TGTGAACCAG GAGCAGATGG GGCTGAGACC 
TTTGCTGATG GTGTACCCCG TGAGGGCTTA TCCCGGCAGC ATGTTCTTAC TCGTATTGGT 
GTTATGTCAC TTATCCGAAA AAAGGTTCAG GAATTTGAGC ATGTCAATGG GCGCTGGAGC 
ATGCCTGAGC TGGCTGAGGT AGAAGAGAAC AAGAAGATGT CACAGCCAGG ATCACCTTCA 
CCTAAAACTC CCACACCTTC CACTCCAGGA GACACACAAC CCAACACTCC TGCACCTGTG 
CCACCCCCTG ATGATGGGGT GAAAGCAGAG GAGAATAATA ACAAAGAAGA AGAGAACACA 
GAAGGAGAGA GAGAGGTGAA ACCTGCAGCT CCTGAGGCTT CTGTGGAGGC TACACAGCCC 
TCTGCATCTA ACCCAGAAGA GGAAAAAGTT CTGACTGAGC CTCTGGAAGG AGAAGAGAAG 
GTGGATAAGC CAGAAGTCAA AGAGAAGACA GAGGAGACCA TGGAGACAGA AACCAAAGGT 
GTTGCTGAAA CGGAAAAGGT AGAGGAGAAA GCAACAGTGG ATCTGACCCC TATTGTGGTA 
GAAGACAAAG AGGAGAAGAA AGAAGAAGAA GAGAAAAAAG AAGTGATGCT TCAGAATGGG 
GAGACTCCCA AGGACCTGGG TGATGAGAAG CAGAAGAAGA ACATCAAGCA GCGTTTTATG 
TTCAACATTG CAGATGGTGG CTTCACTGAG CTGCATTCCC TTTGGCAGAA TGAGGAACGG 
GCAGCCACAG TTACAAAGAA GACCTATGAG ATCTGGCATC GGCGGCATGA CTACTGGCTG 
CTGGCTGGCA TTATTAACCA TGGCTATGCT CGGTGGCAGG ACATTCAGAA TGACCCGCGC 
TATGCCATTC TTAATGAACC CTTCAAAGGT GAAATGAATC GTGGTAACTT CTTGGAGATA 
AAAAACAAAT TCCTGGCTAG AAGATTTAAG GTGAGCAAGG AGAAGAGGAA TATAATCTTT 
GGTTTTTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023360346.1
CDS	79..5610
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERVLLCRQLGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIEPNAEEILQMTKMLDLLEDFLEHEGYKYERIDGEITGNTRQEAIDRFNTPGAQQFCFLLSTRAGGLGISLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTHASVEEHITQEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSHAIIREKEFSFEDNAIHGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELnullLSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIQASGKLLLLQKMLKKLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGEITGNTRQEAIDRFNTPGAQQFCFLLSTRAGGLGISLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTHASVEEHITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVQEEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKVSKEKRNIIFGFL

Target ORF information:

RefSeq Version	XM_023504578.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023504578.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGGTCA TTCAAAAGGC CCTTTCCTAG TGAGTGCCCC ACTTTCTACA 
ATCATCAACT GGGAGCGAGA GTTTGAGATG TGGGCTCCAG ACATGTATGT GGTAACCTAT 
GTTGGAGACA AGGATAGTCG TGCTATTATT CGTGAAAACG AGTTCTCTTT TGAAGACAAT 
GCTATCCGTG GTGGCAAGAA GGCTTCCAGA ATGAAGAAAG AAGCATCTGT GAAGTTTCAT 
GTTTTGCTGA CTTCCTATGA ACTGATTACC ATTGACATGG CCATTCTGGG CTCCATTGAT 
TGGGCCTGCC TAATTGTGGA TGAAGCTCAT AGGCTTAAGA ACAATCAGTC TAAGTTTTTC 
AGGGTACTCA ATGGCTATTC CCTCCAGCAC AAGTTACTAC TAACAGGGAC TCCTCTACAG 
AATAACTTGG AGGAGCTTTT CCACCTGCTT AATTTCCTCA CTCCAGAAAG ATTCCACAAC 
CTGGAGGGCT TCCTAGAAGA ATTTGCTGAC ATTGCCAAGG AAGACCAGAT CAAAAAATTG 
CATGACATGT TGGGGCCTCA TATGCTTCGA CGGCTTAAAG CTGATGTCTT CAAGAACATG 
CCTTCTAAGA CAGAGCTGAT TGAGCCCAAT GCAGAAGAAA TACTACAAAT GACCAAGATG 
TTGGATCTGT TAGAGGATTT TTTGGAACAC GAAGGTTATA AATATGAGCG AATCGATGGG 
GAAATTACTG GGAACACGCG TCAGGAAGCC ATTGATCGTT TTAACACACC TGGTGCTCAG 
CAGTTTTGCT TTCTTCTTTC TACTCGAGCT GGGGGCCTGG GAATCAGTCT AGCTACTGCA 
GATACTGTTA TCATCTATGA CTCTGATTGG AACCCCCATA ATGACATCCA GGCTTTTAGC 
AGAGCCCATC GAATTGGTCA GAATAAGAAA GTGATGATCT ACCGTTTTGT GACCCACGCT 
TCAGTGGAGG AGCACATCAC ACAGGAAAAG GAAGGTATCC AGTGGGAAGC AAAGGAGGAC 
AACTCTGAGG GTGAAGAAAT CCTAGAAGAA GTTGGTGGTG ACCCTGAGGA GGAGGATGAT 
CATCACATGG AGTTTTGCCG TGTCTGCAAG GATGGTGGGG AACTACTCTG CTGTGATACC 
TGCCCCTCTT CCTACCATAT CCACTGCTTG AACCCCCCAC TCCCAGAGAT CCCCAACGGC 
GAATGGCTTT GTCCTCGTTG CACATGCCCT TCACTGAAAG GTAAAGTTCA GAAGATCCTG 
ATTTGGAAAT GGGGGCAGCC ACCATCCCCT ACACCAGTGC CCCGACCCCC TGATGCTGAC 
CCCAATACCC CTTCTCCTAA GCCTTTAGAG GGGCGACCTG AGCGGCAATT CTTTGTGAAA 
TGGCAAGGCA TGTCCTACTG GCACTGTTCT TGGGTGTCAG AACTACAGCT GGAGCTTCAC 
TGCCAAGTGA TGTTCCGTAA CTACCAGCGT AAGAATGATA TGGATGAGCC ACCATCTGGT 
GACTTTGGTG GGGAGGAAGA AAAAAGCCGT AAACGAAAGA ACAAGGACCC CAAGTTTGCA 
GAAATGGAAG AACGTTTCTA CCGCTATGGG ATTAAACCTG AGTGGATGAT GATCCACCGT 
ATCCTCAACC ACAGTGTAGA TAAGAAGGGT CATGTCCACT ATTTGATCAA ATGGAGGGAC 
CTGCCCTATG ATCAGGCATC CTGGGAGAGT GAAGATGTAG AGGTGCAAGA CTATGACCTA 
TTCAAGCAGA GCTATTGGAA CCATAGGGAG CTAATGCGGG GTGAGGAAGG ACGACCTGGC 
AAAAAGCTCA AGAAGGTTAA GCTTAGGAAG TTGGAGAGGC CTCCTGAAAC TCCTACAGTT 
GATCCAACAG TGAAATATGA GCGGCAACCA GAGTACTTAG ATGCCACTGG TGGGACTCTA 
CATCCCTATC AAATGGAGGG ATTGAACTGG TTGCGCTTCT CCTGGGCCCA GGGCACTGAC 
ACTATCTTGG CAGATGAAAT GGGCTTGGGC AAGACGGTTC AGACCGCTGT CTTCCTCTAT 
TCTTTGTACA AGGAGGGTCA TTCAAAAGGC CCTTTCCTAG TGAGTGCCCC ACTTTCTACA 
ATCATCAACT GGGAGCGAGA GTTTGAGATG TGGGCTCCAG ACATGTATGT GGTAACCTAT 
GTTGGAGACA AGGATAGTCA TGCTATTATT CGTGAAAAGG AGTTCTCTTT TGAAGACAAT 
GCTATCCATG GTGGCAAGAA GGCTTCCAGA ATGAAGAAAG AAGCATCTGT GAAGTTTCAT 
GTTTTGCTGA CTTCCTATGA ACTGATTACC ATTGACATGG CCATTCTGGG CTCCATTGAT 
TGGGCCTGCC TAATTGTGGA TGAAGCTCAT AGGCTTTTCA GGGTACTCAA TGGCTATTCC 
CTCCAGCACA AGTTACTACT AACAGGGACT CCTCTACAGA ATAACTTGGA AGAGCTTTTC 
CACCTGCTTA ATTTCCTCAC TCCAGAAAGA TTCCACAACC TGGAGGGCTT CCTAGAAGAA 
TTTGCTGACA TTGCCAAGGA AGACCAGATC AAAAAATTGC ATGACATGTT GGGGCCTCAT 
ATGCTTCGAC GGCTTAAAGC TGATGTCTTC AAGAACATGC CTTCTAAGAC AGAGCTGNNA 
TTGAGCCCAA TGCAGAAGAA ATACTACAAG TACATTCTAA CCCGAAACTT TGAGGCACTC 
AATGCTCGGG GTGGTGGCAA TCAGGTTTCC CTACTTAATG TGGTGATGGA TTTAAAAAAG 
TGCTGTAACC ACCCTTACCT CTTCCCTGTG GCTGCCATGG AAGCACCAAA GATGCCCAAT 
GGGATGTATG ATGGCAGTGC TCTAATTCAA GCATCAGGGA AGCTACTTTT ATTGCAAAAA 
ATGCTCAAGA AACTCAAAGA GGGTGGTCAC CGTGTGCTCA TTTTCTCACA GATGACCAAG 
ATGTTGGATC TGTTAGAGGA TTTTTTGGAA CACGAAGGTT ATAAATATGA GCGAATCGAT 
GGGGAAATTA CTGGGAACAC GCGTCAGGAA GCCATTGATC GTTTTAACAC ACCTGGTGCT 
CAGCAGTTTT GCTTTCTTCT TTCTACTCGA GCTGGGGGCC TGGGAATCAG TCTAGCTACT 
GCAGATACTG TTATCATCTA TGACTCTGAT TGGAACCCCC ATAATGACAT CCAGGCTTTT 
AGCAGAGCCC ATCGAATTGG TCAGAATAAG AAAGTGATGA TCTACCGTTT TGTGACCCAC 
GCTTCAGTGG AGGAGCACAT CACACAGGTG GCGAAGAAGA AGATGATGCT TACACATCTT 
GTAGTAAGGC CTGGCCTGGG CTCTAAGACT GGCTCCATGT CCAAGCAGGA GCTTGATGAT 
ATCCTCAAGT TTGGCACTGA GGAATTATTC AAAGATGAAG CCACTGATGG AGGAGGAGAC 
AACAAGGAAG GAGAAGACAG TAGTGTTATT CACTATGATG ACAAGGCCAT AGAGCGACTG 
CTGGATCGAA ATCAGGATGA AACAGAAGAC ACAGAACTTC AAGGCATGAA TGAATATTTG 
AGTTCCTTCA AAGTGGCCCA GTATGTAGTA CAGGAAGAAG AAATGGGGGA GGAAGAAGAA 
GTAGAAAGAG AGATCATCAA GCAGGAAGAA AGTGTGGATC CTGACTACTG GGAGAAGCTG 
CTGCGACACC ATTATGAGCA GCAGCAAGAG GATCTGGCCC GAAACCTAGG CAAAGGGAAG 
CGAATTCGTA AGCAGGTCAA CTACAACGAT GGCTCCCAGG AGGATCGAGA TTGGCAGGAT 
GACCAGTCAG ACAATCAGTC CGACTACTCA GTGGCCTCAG AGGAAGGCGA TGAGGACTTT 
GATGAGCGTT CAGAAGCTCC CAGACGGCCT AGCCGCAAGG GCCTGCGAAA TGATAAGGAC 
AAACCATTGC CTCCATTGTT GGCTCGTGTT GGTGGAAATA TTGAGGTACT TGGCTTCAAT 
GCCCGCCAGC GGAAGGCTTT CCTCAATGCC ATTATGCGTT ATGGGATGCC TCCTCAGGAT 
GCCTTTACTA CCCAATGGCT TGTGCGGGAC CTGAGGGGCA AGTCAGAGAA AGAGTTCAAG 
GCATATGTGT CTCTCTTTAT GCGTCACCTT TGTGAACCAG GAGCAGATGG GGCTGAGACC 
TTTGCTGATG GTGTACCCCG TGAGGGCTTA TCCCGGCAGC ATGTTCTTAC TCGTATTGGT 
GTTATGTCAC TTATCCGAAA AAAGGTTCAG GAATTTGAGC ATGTCAATGG GCGCTGGAGC 
ATGCCTGAGC TGGCTGAGGT AGAAGAGAAC AAGAAGATGT CACAGCCAGG ATCACCTTCA 
CCTAAAACTC CCACACCTTC CACTCCAGGA GACACACAAC CCAACACTCC TGCACCTGTG 
CCACCCCCTG ATGATGGGGT GAAAGCAGAG GAGAATAATA ACAAAGAAGA AGAGAACACA 
GAAGGAGAGA GAGAGGTGAA ACCTGCAGCT CCTGAGGCTT CTGTGGAGGC TACACAGCCC 
TCTGCATCTA ACCCAGAAGA GGAAAAAGTT CTGACTGAGC CTCTGGAAGG AGAAGAGAAG 
GTGGATAAGC CAGAAGTCAA AGAGAAGACA GAGGAGACCA TGGAGACAGA AACCAAAGGT 
GTTGCTGAAA CGGAAAAGGT AGAGGAGAAA GCAACAGTGG ATCTGACCCC TATTGTGGTA 
GAAGACAAAG AGGAGAAGAA AGAAGAAGAA GAGAAAAAAG AAGTGATGCT TCAGAATGGG 
GAGACTCCCA AGGACCTGGG TGATGAGAAG CAGAAGAAGA ACATCAAGCA GCGTTTTATG 
TTCAACATTG CAGATGGTGG CTTCACTGAG CTGCATTCCC TTTGGCAGAA TGAGGAACGG 
GCAGCCACAG TTACAAAGAA GACCTATGAG ATCTGGCATC GGCGGCATGA CTACTGGCTG 
CTGGCTGGCA TTATTAACCA TGGCTATGCT CGGTGGCAGG ACATTCAGAA TGACCCGCGC 
TATGCCATTC TTAATGAACC CTTCAAAGGT GAAATGAATC GTGGTAACTT CTTGGAGATA 
AAAAACAAAT TCCTGGCTAG AAGATTTAAG GTGAGCAAGG AGAAGAGGAA TATAATCTTT 
GGTTTTTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_031938277.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031794137.1 Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa236821
Clone ID Related Accession (Same CDS sequence)	XM_031938277.1
Accession Version	XM_031938277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG GCGAGGAGGA GGTGACTGCT 
GTGGATGGTT ATGAGACAGA CCACCAGGAC TACTGTGAAG TGTGCCAGCA AGGAGGAGAG 
ATCATCCTGT GTGATACCTG TCCCCGTGCT TACCACATGG TCTGCCTGGA TCCTGACATG 
GAGAAGGCCC CGGAGGGCAA ATGGAGTTGT CCACACTGTG AAAAGGAAGG TATCCAGTGG 
GAAGCAAAGG AGGACAACTC TGAGGGTGAA GAAATCCTAG AAGAAGTTGG TGGTGACCCT 
GAGGAGGAGG ATGATCATCA CATGGAGTTT TGCCGTGTCT GCAAGGATGG TGGGGAACTA 
CTCTGCTGTG ATACCTGCCC CTCTTCCTAC CATATCCACT GCTTGAACCC CCCACTCCCA 
GAGATCCCCA ACGGCGAATG GCTTTGTCCT CGTTGCACAT GCCCTTCACT GAAAGGTAAA 
GTTCAGAAGA TCCTGATTTG GAAATGGGGG CAGCCACCAT CCCCTACACC AGTGCCCCGA 
CCCCCTGATG CTGACCCCAA TACCCCTTCT CCTAAGCCTT TAGAGGGGCG ACCTGAGCGG 
CAATTCTTTG TGAAATGGCA AGGCATGTCC TACTGGCACT GTTCTTGGGT GTCAGAACTA 
CAGCTGGAGC TTCACTGCCA AGTGATGTTC CGTAACTACC AGCGTAAGAA TGATATGGAT 
GAGCCACCAT CTGGTGACTT TGGTGGGGAG GAAGAAAAAA GCCGTAAACG AAAGAACAAG 
GACCCCAAGT TTGCAGAAAT GGAAGAACGT TTCTACCGCT ATGGGATTAA ACCTGAGTGG 
ATGATGATCC ACCGTATCCT CAACCACAGT GTAGATAAGA AGGGTCATGT CCACTATTTG 
ATCAAATGGA GGGACCTGCC CTATGATCAG GCATCCTGGG AGAGTGAAGA TGTAGAGGTG 
CAAGACTATG ACCTATTCAA GCAGAGCTAT TGGAACCATA GGGAGCTAAT GCGGGGTGAG 
GAAGGACGAC CTGGCAAAAA GCTCAAGAAG GTTAAGCTTA GGAAGTTGGA GAGGCCTCCT 
GAAACTCCTA CAGTTGATCC AACAGTGAAA TATGAGCGGC AACCAGAGTA CTTAGATGCC 
ACTGGTGGGA CTCTACATCC CTATCAAATG GAGGGATTGA ACTGGTTGCG CTTCTCCTGG 
GCCCAGGGCA CTGACACTAT CTTGGCAGAT GAAATGGGCT TGGGCAAGAC GGTTCAGACC 
GCTGTCTTCC TCTATTCTTT GTACAAGGAG GGTCATTCAA AAGGCCCTTT CCTAGTGAGT 
GCCCCACTTT CTACAATCAT CAACTGGGAG CGAGAGTTTG AGATGTGGGC TCCAGACATG 
TATGTGGTAA CCTATGTTGG AGACAAGGAT AGTCGTGCTA TTATTCGTGA AAACGAGTTC 
TCTTTTGAAG ACAATGCTAT CCGTGGTGGC AAGAAGGCTT CCAGAATGAA GAAAGAAGCA 
TCTGTGAAGT TTCATGTTTT GCTGACTTCC TATGAACTGA TTACCATTGA CATGGCCATT 
CTGGGCTCCA TTGATTGGGC CTGCCTAATT GTGGATGAAG CTCATAGGCT TAAGAACAAT 
CAGTCTAAGT TTTTCAGGGT ACTCAATGGC TATTCCCTCC AGCACAAGTT ACTACTAACA 
GGGACTCCTC TACAGAATAA CTTGGAGGAG CTTTTCCACC TGCTTAATTT CCTCACTCCA 
GAAAGATTCC ACAACCTGGA GGGCTTCCTA GAAGAATTTG CTGACATTGC CAAGGAAGAC 
CAGATCAAAA AATTGCATGA CATGTTGGGG CCTCATATGC TTCGACGGCT TAAAGCTGAT 
GTCTTCAAGA ACATGCCCTC TAAGACAGAG CTGATTGTGC GTGTGGAACT GAGCCCGATG 
CAGAAGAAAT ACTACAAGTA CATTCTAACC CGAAACTTTG AGGCACTCAA TGCTCGGGGT 
GGTGGTAATC AGGTTTCCCT ACTTAATGTG GTGATGGATC TAAAAAAGTG CTGTAACCAC 
CCTTACCTCT TCCCTGTGGC TGCCATGGAA GCACCAAAGA TGCCCAATGG GATGTATGAT 
GGCAGTGCTC TAATTCGAGC ATCAGGGAAG CTACTTTTAT TGCAAAAAAT GCTCAAGAAC 
CTCAAAGAGG GTGGTCACCG TGTGCTCATT TTCTCGCAGA TGACCAAGAT GTTGGATCTG 
TTAGAGGATT TTTTGGAACA TGAAGGTTAT AAATATGAGC GAATCGATGG GGGAATTACT 
GGGAACATGC GTCAGGAAGC CATTGATCGT TTTAACGCAC CTGGGGCTCA GCAGTTTTGC 
TTTCTTCTTT CTACTCGAGC TGGGGGCCTG GGAATCAATC TAGCTACTGC AGATACTGTT 
ATCATCTATG ACTCTGATTG GAACCCCCAT AATGACATCC AGGCTTTTAG CAGAGCCCAT 
CGAATTGGTC AGAATAAGAA AGTGATGATC TACCGTTTTG TGACCCGCGC TTCAGTGGAG 
GAGCGCATCA CACAGGTGGC GAAGAAGAAG ATGATGCTTA CACATCTTGT AGTAAGGCCT 
GGCCTGGGCT CTAAGACTGG CTCCATGTCC AAGCAGGAGC TTGATGATAT CCTCAAGTTT 
GGCACTGAGG AATTATTCAA AGATGAAGCC ACTGATGGAG GAGGAGACAA CAAGGAAGGA 
GAAGACAGTA GTGTTATTCA CTATGATGAC AAGGCCATAG AGCGACTGCT GGATCGAAAT 
CAGGATGAAA CAGAAGACAC AGAACTTCAA GGCATGAATG AATATTTGAG TTCCTTCAAA 
GTGGCCCAGT ATGTAGTACG GGAAGAAGAA ATGGGGGAGG AAGAAGAAGT AGAAAGAGAG 
ATCATCAAGC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAGCTGCT GCGACACCAT 
TATGAGCAGC AGCAAGAGGA TCTGGCCCGA AACCTAGGCA AAGGGAAGCG AATTCGTAAG 
CAGGTCAACT ACAACGATGG CTCCCAGGAG GATCGAGGTG TGTCTGGCCG GCCCCATCCC 
CTGCCCTTGG GCCGCTCCCC TAGGGCCGTG GGCCCCGCTC ATCTGCCCTC TCTCCCTCCA 
GATTGGCAGG ATGACCAGTC AGACAATCAG TCCGACTACT CAGTGGCCTC AGAGGAAGGC 
GATGAGGACT TTGATGAGCG TTCAGAAGCT CCCAGACGGC CTAGCCGCAA GGGCCTGCGA 
AATGATAAGG ACAAACCATT GCCTCCATTG TTGGCTCGTG TTGGTGGAAA TATTGAGGTA 
CTTGGCTTCA ATGCCCGCCA GCGGAAGGCT TTCCTCAATG CCATTATGCG TTATGGGATG 
CCTCCTCAGG ATGCCTTTAC TACCCAATGG CTTGTGCGGG ACCTGAGGGG CAAGTCAGAG 
AAAGAGTTCA AGGCATATGT GTCTCTCTTT ATGCGTCACC TTTGTGAACC AGGAGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTACCC CGTGAGGGCT TATCCCGGCA GCATGTTCTT 
ACTCGTATTG GTGTTATGTC ACTTATCCGA AAAAAGGTTC AGGAATTTGA GCATGTCAAT 
GGGCGCTGGA GCATGCCTGA GCTGGCTGAG GTAGAAGAGA ACAAGAAGAT GTCACAGCCA 
GGATCACCTT CACCTAAAAC TCCCACACCT TCCACTCCAG GAGACACACA ACCCAACACT 
CCTGCACCTG TGCCACCCCC TGATGATGGG GTGAAAGCAG AGGAGAATAA TAACAAAGAA 
GAAGAGAACA CAGAAGGAGA GAGAGAGGTG AAACCTGCAG CTCCTGAGGC TTCTGTGGAG 
GCTACACAGC CCTCTGCATC TAACCCAGAA GAGGAAAAAG TTCTGACTGA GCCTCTGGAA 
GGAGAAGAGA AGGTGGATAA GCCAGAAGTC AAAGAGAAGA CAGAGGAGAC CATGGAGACA 
GAAACCAAAG GTGTTGCTGA AACGGAAAAG GTAGAGGAGA AAGCAACAGT GGATCTGACC 
CCTATTGTGG TAGAAGACAA AGAGGAGAAG AAAGAAGAAG AAGAGAAAAA AGAAGTGATG 
CTTCAGAATG GGGAGACTCC CAAGGACCTG GGTGATGAGA AGCAGAAGAA GAACATCAAG 
CAGCGTTTTA TGTTCAACAT TGCAGATGGT GGCTTCACTG AGCTGCATTC CCTTTGGCAG 
AATGAGGAAC GGGCAGCCAC AGTTACAAAG AAGACCTATG AGATCTGGCA TCGGCGGCAT 
GACTACTGGC TGCTGGCTGG CATTATTAAC CATGGCTATG CTCGGTGGCA GGACATTCAG 
AATGACCCGC GCTATGCCAT TCTTAATGAA CCCTTCAAAG GTGAAATGAA TCGTGGTAAC 
TTCTTGGAGA TAAAAAACAA ATTCCTGGCT AGAAGATTTA AGCTCTTGGA ACAAGCCTTG 
GTGATTGAGG AGCAACTGCG TCGAGCAGCT TACCTGAACA TGTCAGAGGA CCCTTCCCAT 
CCTTCCATGG CCCTCAATAC TCGTTTTGCT GAAGTGGAAT GCCTGGCTGA GAGCCACCAG 
CACCTGTCTA AGGAGTCAAT GGCAGGAAAT AAGCCAGCTA ATGCTGTCCT GCACAAAGTT 
CTGAAACAAC TGGAGGAATT GTTGAGTGAC ATGAAGGCTG ATGTCACAAG GCTGCCAGCC 
ACAATTGCCC GTATTCCCCC TGTGGCTGTG CGGCTACAGA TGTCAGAGCG CAACATCCTC 
AGCCGGCTGG CAAACCGGGG CCCTGAACCC ACCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031794137.1
CDS	220..6039
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERVLLCRQLGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKEPKSSGQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKASPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRATKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVSGRPHPLPLGRSPRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRGPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_031938277.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031938277.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG GCGAGGAGGA GGTGACTGCT 
GTGGATGGTT ATGAGACAGA CCACCAGGAC TACTGTGAAG TGTGCCAGCA AGGAGGAGAG 
ATCATCCTGT GTGATACCTG TCCCCGTGCT TACCACATGG TCTGCCTGGA TCCTGACATG 
GAGAAGGCCC CGGAGGGCAA ATGGAGTTGT CCACACTGTG AAAAGGAAGG TATCCAGTGG 
GAAGCAAAGG AGGACAACTC TGAGGGTGAA GAAATCCTAG AAGAAGTTGG TGGTGACCCT 
GAGGAGGAGG ATGATCATCA CATGGAGTTT TGCCGTGTCT GCAAGGATGG TGGGGAACTA 
CTCTGCTGTG ATACCTGCCC CTCTTCCTAC CATATCCACT GCTTGAACCC CCCACTCCCA 
GAGATCCCCA ACGGCGAATG GCTTTGTCCT CGTTGCACAT GCCCTTCACT GAAAGGTAAA 
GTTCAGAAGA TCCTGATTTG GAAATGGGGG CAGCCACCAT CCCCTACACC AGTGCCCCGA 
CCCCCTGATG CTGACCCCAA TACCCCTTCT CCTAAGCCTT TAGAGGGGCG ACCTGAGCGG 
CAATTCTTTG TGAAATGGCA AGGCATGTCC TACTGGCACT GTTCTTGGGT GTCAGAACTA 
CAGCTGGAGC TTCACTGCCA AGTGATGTTC CGTAACTACC AGCGTAAGAA TGATATGGAT 
GAGCCACCAT CTGGTGACTT TGGTGGGGAG GAAGAAAAAA GCCGTAAACG AAAGAACAAG 
GACCCCAAGT TTGCAGAAAT GGAAGAACGT TTCTACCGCT ATGGGATTAA ACCTGAGTGG 
ATGATGATCC ACCGTATCCT CAACCACAGT GTAGATAAGA AGGGTCATGT CCACTATTTG 
ATCAAATGGA GGGACCTGCC CTATGATCAG GCATCCTGGG AGAGTGAAGA TGTAGAGGTG 
CAAGACTATG ACCTATTCAA GCAGAGCTAT TGGAACCATA GGGAGCTAAT GCGGGGTGAG 
GAAGGACGAC CTGGCAAAAA GCTCAAGAAG GTTAAGCTTA GGAAGTTGGA GAGGCCTCCT 
GAAACTCCTA CAGTTGATCC AACAGTGAAA TATGAGCGGC AACCAGAGTA CTTAGATGCC 
ACTGGTGGGA CTCTACATCC CTATCAAATG GAGGGATTGA ACTGGTTGCG CTTCTCCTGG 
GCCCAGGGCA CTGACACTAT CTTGGCAGAT GAAATGGGCT TGGGCAAGAC GGTTCAGACC 
GCTGTCTTCC TCTATTCTTT GTACAAGGAG GGTCATTCAA AAGGCCCTTT CCTAGTGAGT 
GCCCCACTTT CTACAATCAT CAACTGGGAG CGAGAGTTTG AGATGTGGGC TCCAGACATG 
TATGTGGTAA CCTATGTTGG AGACAAGGAT AGTCGTGCTA TTATTCGTGA AAACGAGTTC 
TCTTTTGAAG ACAATGCTAT CCGTGGTGGC AAGAAGGCTT CCAGAATGAA GAAAGAAGCA 
TCTGTGAAGT TTCATGTTTT GCTGACTTCC TATGAACTGA TTACCATTGA CATGGCCATT 
CTGGGCTCCA TTGATTGGGC CTGCCTAATT GTGGATGAAG CTCATAGGCT TAAGAACAAT 
CAGTCTAAGT TTTTCAGGGT ACTCAATGGC TATTCCCTCC AGCACAAGTT ACTACTAACA 
GGGACTCCTC TACAGAATAA CTTGGAGGAG CTTTTCCACC TGCTTAATTT CCTCACTCCA 
GAAAGATTCC ACAACCTGGA GGGCTTCCTA GAAGAATTTG CTGACATTGC CAAGGAAGAC 
CAGATCAAAA AATTGCATGA CATGTTGGGG CCTCATATGC TTCGACGGCT TAAAGCTGAT 
GTCTTCAAGA ACATGCCCTC TAAGACAGAG CTGATTGTGC GTGTGGAACT GAGCCCGATG 
CAGAAGAAAT ACTACAAGTA CATTCTAACC CGAAACTTTG AGGCACTCAA TGCTCGGGGT 
GGTGGTAATC AGGTTTCCCT ACTTAATGTG GTGATGGATC TAAAAAAGTG CTGTAACCAC 
CCTTACCTCT TCCCTGTGGC TGCCATGGAA GCACCAAAGA TGCCCAATGG GATGTATGAT 
GGCAGTGCTC TAATTCGAGC ATCAGGGAAG CTACTTTTAT TGCAAAAAAT GCTCAAGAAC 
CTCAAAGAGG GTGGTCACCG TGTGCTCATT TTCTCGCAGA TGACCAAGAT GTTGGATCTG 
TTAGAGGATT TTTTGGAACA TGAAGGTTAT AAATATGAGC GAATCGATGG GGGAATTACT 
GGGAACATGC GTCAGGAAGC CATTGATCGT TTTAACGCAC CTGGGGCTCA GCAGTTTTGC 
TTTCTTCTTT CTACTCGAGC TGGGGGCCTG GGAATCAATC TAGCTACTGC AGATACTGTT 
ATCATCTATG ACTCTGATTG GAACCCCCAT AATGACATCC AGGCTTTTAG CAGAGCCCAT 
CGAATTGGTC AGAATAAGAA AGTGATGATC TACCGTTTTG TGACCCGCGC TTCAGTGGAG 
GAGCGCATCA CACAGGTGGC GAAGAAGAAG ATGATGCTTA CACATCTTGT AGTAAGGCCT 
GGCCTGGGCT CTAAGACTGG CTCCATGTCC AAGCAGGAGC TTGATGATAT CCTCAAGTTT 
GGCACTGAGG AATTATTCAA AGATGAAGCC ACTGATGGAG GAGGAGACAA CAAGGAAGGA 
GAAGACAGTA GTGTTATTCA CTATGATGAC AAGGCCATAG AGCGACTGCT GGATCGAAAT 
CAGGATGAAA CAGAAGACAC AGAACTTCAA GGCATGAATG AATATTTGAG TTCCTTCAAA 
GTGGCCCAGT ATGTAGTACG GGAAGAAGAA ATGGGGGAGG AAGAAGAAGT AGAAAGAGAG 
ATCATCAAGC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAGCTGCT GCGACACCAT 
TATGAGCAGC AGCAAGAGGA TCTGGCCCGA AACCTAGGCA AAGGGAAGCG AATTCGTAAG 
CAGGTCAACT ACAACGATGG CTCCCAGGAG GATCGAGGTG TGTCTGGCCG GCCCCATCCC 
CTGCCCTTGG GCCGCTCCCC TAGGGCCGTG GGCCCCGCTC ATCTGCCCTC TCTCCCTCCA 
GATTGGCAGG ATGACCAGTC AGACAATCAG TCCGACTACT CAGTGGCCTC AGAGGAAGGC 
GATGAGGACT TTGATGAGCG TTCAGAAGCT CCCAGACGGC CTAGCCGCAA GGGCCTGCGA 
AATGATAAGG ACAAACCATT GCCTCCATTG TTGGCTCGTG TTGGTGGAAA TATTGAGGTA 
CTTGGCTTCA ATGCCCGCCA GCGGAAGGCT TTCCTCAATG CCATTATGCG TTATGGGATG 
CCTCCTCAGG ATGCCTTTAC TACCCAATGG CTTGTGCGGG ACCTGAGGGG CAAGTCAGAG 
AAAGAGTTCA AGGCATATGT GTCTCTCTTT ATGCGTCACC TTTGTGAACC AGGAGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTACCC CGTGAGGGCT TATCCCGGCA GCATGTTCTT 
ACTCGTATTG GTGTTATGTC ACTTATCCGA AAAAAGGTTC AGGAATTTGA GCATGTCAAT 
GGGCGCTGGA GCATGCCTGA GCTGGCTGAG GTAGAAGAGA ACAAGAAGAT GTCACAGCCA 
GGATCACCTT CACCTAAAAC TCCCACACCT TCCACTCCAG GAGACACACA ACCCAACACT 
CCTGCACCTG TGCCACCCCC TGATGATGGG GTGAAAGCAG AGGAGAATAA TAACAAAGAA 
GAAGAGAACA CAGAAGGAGA GAGAGAGGTG AAACCTGCAG CTCCTGAGGC TTCTGTGGAG 
GCTACACAGC CCTCTGCATC TAACCCAGAA GAGGAAAAAG TTCTGACTGA GCCTCTGGAA 
GGAGAAGAGA AGGTGGATAA GCCAGAAGTC AAAGAGAAGA CAGAGGAGAC CATGGAGACA 
GAAACCAAAG GTGTTGCTGA AACGGAAAAG GTAGAGGAGA AAGCAACAGT GGATCTGACC 
CCTATTGTGG TAGAAGACAA AGAGGAGAAG AAAGAAGAAG AAGAGAAAAA AGAAGTGATG 
CTTCAGAATG GGGAGACTCC CAAGGACCTG GGTGATGAGA AGCAGAAGAA GAACATCAAG 
CAGCGTTTTA TGTTCAACAT TGCAGATGGT GGCTTCACTG AGCTGCATTC CCTTTGGCAG 
AATGAGGAAC GGGCAGCCAC AGTTACAAAG AAGACCTATG AGATCTGGCA TCGGCGGCAT 
GACTACTGGC TGCTGGCTGG CATTATTAAC CATGGCTATG CTCGGTGGCA GGACATTCAG 
AATGACCCGC GCTATGCCAT TCTTAATGAA CCCTTCAAAG GTGAAATGAA TCGTGGTAAC 
TTCTTGGAGA TAAAAAACAA ATTCCTGGCT AGAAGATTTA AGCTCTTGGA ACAAGCCTTG 
GTGATTGAGG AGCAACTGCG TCGAGCAGCT TACCTGAACA TGTCAGAGGA CCCTTCCCAT 
CCTTCCATGG CCCTCAATAC TCGTTTTGCT GAAGTGGAAT GCCTGGCTGA GAGCCACCAG 
CACCTGTCTA AGGAGTCAAT GGCAGGAAAT AAGCCAGCTA ATGCTGTCCT GCACAAAGTT 
CTGAAACAAC TGGAGGAATT GTTGAGTGAC ATGAAGGCTG ATGTCACAAG GCTGCCAGCC 
ACAATTGCCC GTATTCCCCC TGTGGCTGTG CGGCTACAGA TGTCAGAGCG CAACATCCTC 
AGCCGGCTGG CAAACCGGGG CCCTGAACCC ACCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_031938278.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031794138.1 Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa236822
Clone ID Related Accession (Same CDS sequence)	XM_031938278.1
Accession Version	XM_031938278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG GCGAGGAGGA GGTGACTGCT 
GTGGATGGTT ATGAGACAGA CCACCAGGAC TACTGTGAAG TGTGCCAGCA AGGAGGAGAG 
ATCATCCTGT GTGATACCTG TCCCCGTGCT TACCACATGG TCTGCCTGGA TCCTGACATG 
GAGAAGGCCC CGGAGGGCAA ATGGAGTTGT CCACACTGTG AAAAGGAAGG TATCCAGTGG 
GAAGCAAAGG AGGACAACTC TGAGGGTGAA GAAATCCTAG AAGAAGTTGG TGGTGACCCT 
GAGGAGGAGG ATGATCATCA CATGGAGTTT TGCCGTGTCT GCAAGGATGG TGGGGAACTA 
CTCTGCTGTG ATACCTGCCC CTCTTCCTAC CATATCCACT GCTTGAACCC CCCACTCCCA 
GAGATCCCCA ACGGCGAATG GCTTTGTCCT CGTTGCACAT GCCCTTCACT GAAAGGTAAA 
GTTCAGAAGA TCCTGATTTG GAAATGGGGG CAGCCACCAT CCCCTACACC AGTGCCCCGA 
CCCCCTGATG CTGACCCCAA TACCCCTTCT CCTAAGCCTT TAGAGGGGCG ACCTGAGCGG 
CAATTCTTTG TGAAATGGCA AGGCATGTCC TACTGGCACT GTTCTTGGGT GTCAGAACTA 
CAGCTGGAGC TTCACTGCCA AGTGATGTTC CGTAACTACC AGCGTAAGAA TGATATGGAT 
GAGCCACCAT CTGGTGACTT TGGTGGGGAG GAAGAAAAAA GCCGTAAACG AAAGAACAAG 
GACCCCAAGT TTGCAGAAAT GGAAGAACGT TTCTACCGCT ATGGGATTAA ACCTGAGTGG 
ATGATGATCC ACCGTATCCT CAACCACAGT GTAGATAAGA AGGGTCATGT CCACTATTTG 
ATCAAATGGA GGGACCTGCC CTATGATCAG GCATCCTGGG AGAGTGAAGA TGTAGAGGTG 
CAAGACTATG ACCTATTCAA GCAGAGCTAT TGGAACCATA GGGAGCTAAT GCGGGGTGAG 
GAAGGACGAC CTGGCAAAAA GCTCAAGAAG GTTAAGCTTA GGAAGTTGGA GAGGCCTCCT 
GAAACTCCTA CAGTTGATCC AACAGTGAAA TATGAGCGGC AACCAGAGTA CTTAGATGCC 
ACTGGTGGGA CTCTACATCC CTATCAAATG GAGGGATTGA ACTGGTTGCG CTTCTCCTGG 
GCCCAGGGCA CTGACACTAT CTTGGCAGAT GAAATGGGCT TGGGCAAGAC GGTTCAGACC 
GCTGTCTTCC TCTATTCTTT GTACAAGGAG GGTCATTCAA AAGGCCCTTT CCTAGTGAGT 
GCCCCACTTT CTACAATCAT CAACTGGGAG CGAGAGTTTG AGATGTGGGC TCCAGACATG 
TATGTGGTAA CCTATGTTGG AGACAAGGAT AGTCGTGCTA TTATTCGTGA AAACGAGTTC 
TCTTTTGAAG ACAATGCTAT CCGTGGTGGC AAGAAGGCTT CCAGAATGAA GAAAGAAGCA 
TCTGTGAAGT TTCATGTTTT GCTGACTTCC TATGAACTGA TTACCATTGA CATGGCCATT 
CTGGGCTCCA TTGATTGGGC CTGCCTAATT GTGGATGAAG CTCATAGGCT TAAGAACAAT 
CAGTCTAAGT TTTTCAGGGT ACTCAATGGC TATTCCCTCC AGCACAAGTT ACTACTAACA 
GGGACTCCTC TACAGAATAA CTTGGAGGAG CTTTTCCACC TGCTTAATTT CCTCACTCCA 
GAAAGATTCC ACAACCTGGA GGGCTTCCTA GAAGAATTTG CTGACATTGC CAAGGAAGAC 
CAGATCAAAA AATTGCATGA CATGTTGGGG CCTCATATGC TTCGACGGCT TAAAGCTGAT 
GTCTTCAAGA ACATGCCCTC TAAGACAGAG CTGATTGTGC GTGTGGAACT GAGCCCGATG 
CAGAAGAAAT ACTACAAGTA CATTCTAACC CGAAACTTTG AGGCACTCAA TGCTCGGGGT 
GGTGGTAATC AGGTTTCCCT ACTTAATGTG GTGATGGATC TAAAAAAGTG CTGTAACCAC 
CCTTACCTCT TCCCTGTGGC TGCCATGGAA GCACCAAAGA TGCCCAATGG GATGTATGAT 
GGCAGTGCTC TAATTCGAGC ATCAGGGAAG CTACTTTTAT TGCAAAAAAT GCTCAAGAAC 
CTCAAAGAGG GTGGTCACCG TGTGCTCATT TTCTCGCAGA TGACCAAGAT GTTGGATCTG 
TTAGAGGATT TTTTGGAACA TGAAGGTTAT AAATATGAGC GAATCGATGG GGGAATTACT 
GGGAACATGC GTCAGGAAGC CATTGATCGT TTTAACGCAC CTGGGGCTCA GCAGTTTTGC 
TTTCTTCTTT CTACTCGAGC TGGGGGCCTG GGAATCAATC TAGCTACTGC AGATACTGTT 
ATCATCTATG ACTCTGATTG GAACCCCCAT AATGACATCC AGGCTTTTAG CAGAGCCCAT 
CGAATTGGTC AGAATAAGAA AGTGATGATC TACCGTTTTG TGACCCGCGC TTCAGTGGAG 
GAGCGCATCA CACAGGTGGC GAAGAAGAAG ATGATGCTTA CACATCTTGT AGTAAGGCCT 
GGCCTGGGCT CTAAGACTGG CTCCATGTCC AAGCAGGAGC TTGATGATAT CCTCAAGTTT 
GGCACTGAGG AATTATTCAA AGATGAAGCC ACTGATGGAG GAGGAGACAA CAAGGAAGGA 
GAAGACAGTA GTGTTATTCA CTATGATGAC AAGGCCATAG AGCGACTGCT GGATCGAAAT 
CAGGATGAAA CAGAAGACAC AGAACTTCAA GGCATGAATG AATATTTGAG TTCCTTCAAA 
GTGGCCCAGT ATGTAGTACG GGAAGAAGAA ATGGGGGAGG AAGAAGAAGT AGAAAGAGAG 
ATCATCAAGC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAGCTGCT GCGACACCAT 
TATGAGCAGC AGCAAGAGGA TCTGGCCCGA AACCTAGGCA AAGGGAAGCG AATTCGTAAG 
CAGGTCAACT ACAACGATGG CTCCCAGGAG GATCGAGATT GGCAGGATGA CCAGTCAGAC 
AATCAGTCCG ACTACTCAGT GGCCTCAGAG GAAGGCGATG AGGACTTTGA TGAGCGTTCA 
GAAGCTCCCA GACGGCCTAG CCGCAAGGGC CTGCGAAATG ATAAGGACAA ACCATTGCCT 
CCATTGTTGG CTCGTGTTGG TGGAAATATT GAGGTACTTG GCTTCAATGC CCGCCAGCGG 
AAGGCTTTCC TCAATGCCAT TATGCGTTAT GGGATGCCTC CTCAGGATGC CTTTACTACC 
CAATGGCTTG TGCGGGACCT GAGGGGCAAG TCAGAGAAAG AGTTCAAGGC ATATGTGTCT 
CTCTTTATGC GTCACCTTTG TGAACCAGGA GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTACCCCGTG AGGGCTTATC CCGGCAGCAT GTTCTTACTC GTATTGGTGT TATGTCACTT 
ATCCGAAAAA AGGTTCAGGA ATTTGAGCAT GTCAATGGGC GCTGGAGCAT GCCTGAGCTG 
GCTGAGGTAG AAGAGAACAA GAAGATGTCA CAGCCAGGAT CACCTTCACC TAAAACTCCC 
ACACCTTCCA CTCCAGGAGA CACACAACCC AACACTCCTG CACCTGTGCC ACCCCCTGAT 
GATGGGGTGA AAGCAGAGGA GAATAATAAC AAAGAAGAAG AGAACACAGA AGGAGAGAGA 
GAGGTGAAAC CTGCAGCTCC TGAGGCTTCT GTGGAGGCTA CACAGCCCTC TGCATCTAAC 
CCAGAAGAGG AAAAAGTTCT GACTGAGCCT CTGGAAGGAG AAGAGAAGGT GGATAAGCCA 
GAAGTCAAAG AGAAGACAGA GGAGACCATG GAGACAGAAA CCAAAGGTGT TGCTGAAACG 
GAAAAGGTAG AGGAGAAAGC AACAGTGGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAG 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAA GTGATGCTTC AGAATGGGGA GACTCCCAAG 
GACCTGGGTG ATGAGAAGCA GAAGAAGAAC ATCAAGCAGC GTTTTATGTT CAACATTGCA 
GATGGTGGCT TCACTGAGCT GCATTCCCTT TGGCAGAATG AGGAACGGGC AGCCACAGTT 
ACAAAGAAGA CCTATGAGAT CTGGCATCGG CGGCATGACT ACTGGCTGCT GGCTGGCATT 
ATTAACCATG GCTATGCTCG GTGGCAGGAC ATTCAGAATG ACCCGCGCTA TGCCATTCTT 
AATGAACCCT TCAAAGGTGA AATGAATCGT GGTAACTTCT TGGAGATAAA AAACAAATTC 
CTGGCTAGAA GATTTAAGCT CTTGGAACAA GCCTTGGTGA TTGAGGAGCA ACTGCGTCGA 
GCAGCTTACC TGAACATGTC AGAGGACCCT TCCCATCCTT CCATGGCCCT CAATACTCGT 
TTTGCTGAAG TGGAATGCCT GGCTGAGAGC CACCAGCACC TGTCTAAGGA GTCAATGGCA 
GGAAATAAGC CAGCTAATGC TGTCCTGCAC AAAGGTATTC TGAAACAACT GGAGGAATTG 
TTGAGTGACA TGAAGGCTGA TGTCACAAGG CTGCCAGCCA CAATTGCCCG TATTCCCCCT 
GTGGCTGTGC GGCTACAGAT GTCAGAGCGC AACATCCTCA GCCGGCTGGC AAACCGGGGC 
CCTGAACCCA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031794138.1
CDS	220..5958
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERVLLCRQLGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKEPKSSGQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKASPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRATKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRGPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_031938278.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031938278.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG GCGAGGAGGA GGTGACTGCT 
GTGGATGGTT ATGAGACAGA CCACCAGGAC TACTGTGAAG TGTGCCAGCA AGGAGGAGAG 
ATCATCCTGT GTGATACCTG TCCCCGTGCT TACCACATGG TCTGCCTGGA TCCTGACATG 
GAGAAGGCCC CGGAGGGCAA ATGGAGTTGT CCACACTGTG AAAAGGAAGG TATCCAGTGG 
GAAGCAAAGG AGGACAACTC TGAGGGTGAA GAAATCCTAG AAGAAGTTGG TGGTGACCCT 
GAGGAGGAGG ATGATCATCA CATGGAGTTT TGCCGTGTCT GCAAGGATGG TGGGGAACTA 
CTCTGCTGTG ATACCTGCCC CTCTTCCTAC CATATCCACT GCTTGAACCC CCCACTCCCA 
GAGATCCCCA ACGGCGAATG GCTTTGTCCT CGTTGCACAT GCCCTTCACT GAAAGGTAAA 
GTTCAGAAGA TCCTGATTTG GAAATGGGGG CAGCCACCAT CCCCTACACC AGTGCCCCGA 
CCCCCTGATG CTGACCCCAA TACCCCTTCT CCTAAGCCTT TAGAGGGGCG ACCTGAGCGG 
CAATTCTTTG TGAAATGGCA AGGCATGTCC TACTGGCACT GTTCTTGGGT GTCAGAACTA 
CAGCTGGAGC TTCACTGCCA AGTGATGTTC CGTAACTACC AGCGTAAGAA TGATATGGAT 
GAGCCACCAT CTGGTGACTT TGGTGGGGAG GAAGAAAAAA GCCGTAAACG AAAGAACAAG 
GACCCCAAGT TTGCAGAAAT GGAAGAACGT TTCTACCGCT ATGGGATTAA ACCTGAGTGG 
ATGATGATCC ACCGTATCCT CAACCACAGT GTAGATAAGA AGGGTCATGT CCACTATTTG 
ATCAAATGGA GGGACCTGCC CTATGATCAG GCATCCTGGG AGAGTGAAGA TGTAGAGGTG 
CAAGACTATG ACCTATTCAA GCAGAGCTAT TGGAACCATA GGGAGCTAAT GCGGGGTGAG 
GAAGGACGAC CTGGCAAAAA GCTCAAGAAG GTTAAGCTTA GGAAGTTGGA GAGGCCTCCT 
GAAACTCCTA CAGTTGATCC AACAGTGAAA TATGAGCGGC AACCAGAGTA CTTAGATGCC 
ACTGGTGGGA CTCTACATCC CTATCAAATG GAGGGATTGA ACTGGTTGCG CTTCTCCTGG 
GCCCAGGGCA CTGACACTAT CTTGGCAGAT GAAATGGGCT TGGGCAAGAC GGTTCAGACC 
GCTGTCTTCC TCTATTCTTT GTACAAGGAG GGTCATTCAA AAGGCCCTTT CCTAGTGAGT 
GCCCCACTTT CTACAATCAT CAACTGGGAG CGAGAGTTTG AGATGTGGGC TCCAGACATG 
TATGTGGTAA CCTATGTTGG AGACAAGGAT AGTCGTGCTA TTATTCGTGA AAACGAGTTC 
TCTTTTGAAG ACAATGCTAT CCGTGGTGGC AAGAAGGCTT CCAGAATGAA GAAAGAAGCA 
TCTGTGAAGT TTCATGTTTT GCTGACTTCC TATGAACTGA TTACCATTGA CATGGCCATT 
CTGGGCTCCA TTGATTGGGC CTGCCTAATT GTGGATGAAG CTCATAGGCT TAAGAACAAT 
CAGTCTAAGT TTTTCAGGGT ACTCAATGGC TATTCCCTCC AGCACAAGTT ACTACTAACA 
GGGACTCCTC TACAGAATAA CTTGGAGGAG CTTTTCCACC TGCTTAATTT CCTCACTCCA 
GAAAGATTCC ACAACCTGGA GGGCTTCCTA GAAGAATTTG CTGACATTGC CAAGGAAGAC 
CAGATCAAAA AATTGCATGA CATGTTGGGG CCTCATATGC TTCGACGGCT TAAAGCTGAT 
GTCTTCAAGA ACATGCCCTC TAAGACAGAG CTGATTGTGC GTGTGGAACT GAGCCCGATG 
CAGAAGAAAT ACTACAAGTA CATTCTAACC CGAAACTTTG AGGCACTCAA TGCTCGGGGT 
GGTGGTAATC AGGTTTCCCT ACTTAATGTG GTGATGGATC TAAAAAAGTG CTGTAACCAC 
CCTTACCTCT TCCCTGTGGC TGCCATGGAA GCACCAAAGA TGCCCAATGG GATGTATGAT 
GGCAGTGCTC TAATTCGAGC ATCAGGGAAG CTACTTTTAT TGCAAAAAAT GCTCAAGAAC 
CTCAAAGAGG GTGGTCACCG TGTGCTCATT TTCTCGCAGA TGACCAAGAT GTTGGATCTG 
TTAGAGGATT TTTTGGAACA TGAAGGTTAT AAATATGAGC GAATCGATGG GGGAATTACT 
GGGAACATGC GTCAGGAAGC CATTGATCGT TTTAACGCAC CTGGGGCTCA GCAGTTTTGC 
TTTCTTCTTT CTACTCGAGC TGGGGGCCTG GGAATCAATC TAGCTACTGC AGATACTGTT 
ATCATCTATG ACTCTGATTG GAACCCCCAT AATGACATCC AGGCTTTTAG CAGAGCCCAT 
CGAATTGGTC AGAATAAGAA AGTGATGATC TACCGTTTTG TGACCCGCGC TTCAGTGGAG 
GAGCGCATCA CACAGGTGGC GAAGAAGAAG ATGATGCTTA CACATCTTGT AGTAAGGCCT 
GGCCTGGGCT CTAAGACTGG CTCCATGTCC AAGCAGGAGC TTGATGATAT CCTCAAGTTT 
GGCACTGAGG AATTATTCAA AGATGAAGCC ACTGATGGAG GAGGAGACAA CAAGGAAGGA 
GAAGACAGTA GTGTTATTCA CTATGATGAC AAGGCCATAG AGCGACTGCT GGATCGAAAT 
CAGGATGAAA CAGAAGACAC AGAACTTCAA GGCATGAATG AATATTTGAG TTCCTTCAAA 
GTGGCCCAGT ATGTAGTACG GGAAGAAGAA ATGGGGGAGG AAGAAGAAGT AGAAAGAGAG 
ATCATCAAGC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAGCTGCT GCGACACCAT 
TATGAGCAGC AGCAAGAGGA TCTGGCCCGA AACCTAGGCA AAGGGAAGCG AATTCGTAAG 
CAGGTCAACT ACAACGATGG CTCCCAGGAG GATCGAGATT GGCAGGATGA CCAGTCAGAC 
AATCAGTCCG ACTACTCAGT GGCCTCAGAG GAAGGCGATG AGGACTTTGA TGAGCGTTCA 
GAAGCTCCCA GACGGCCTAG CCGCAAGGGC CTGCGAAATG ATAAGGACAA ACCATTGCCT 
CCATTGTTGG CTCGTGTTGG TGGAAATATT GAGGTACTTG GCTTCAATGC CCGCCAGCGG 
AAGGCTTTCC TCAATGCCAT TATGCGTTAT GGGATGCCTC CTCAGGATGC CTTTACTACC 
CAATGGCTTG TGCGGGACCT GAGGGGCAAG TCAGAGAAAG AGTTCAAGGC ATATGTGTCT 
CTCTTTATGC GTCACCTTTG TGAACCAGGA GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTACCCCGTG AGGGCTTATC CCGGCAGCAT GTTCTTACTC GTATTGGTGT TATGTCACTT 
ATCCGAAAAA AGGTTCAGGA ATTTGAGCAT GTCAATGGGC GCTGGAGCAT GCCTGAGCTG 
GCTGAGGTAG AAGAGAACAA GAAGATGTCA CAGCCAGGAT CACCTTCACC TAAAACTCCC 
ACACCTTCCA CTCCAGGAGA CACACAACCC AACACTCCTG CACCTGTGCC ACCCCCTGAT 
GATGGGGTGA AAGCAGAGGA GAATAATAAC AAAGAAGAAG AGAACACAGA AGGAGAGAGA 
GAGGTGAAAC CTGCAGCTCC TGAGGCTTCT GTGGAGGCTA CACAGCCCTC TGCATCTAAC 
CCAGAAGAGG AAAAAGTTCT GACTGAGCCT CTGGAAGGAG AAGAGAAGGT GGATAAGCCA 
GAAGTCAAAG AGAAGACAGA GGAGACCATG GAGACAGAAA CCAAAGGTGT TGCTGAAACG 
GAAAAGGTAG AGGAGAAAGC AACAGTGGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAG 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAA GTGATGCTTC AGAATGGGGA GACTCCCAAG 
GACCTGGGTG ATGAGAAGCA GAAGAAGAAC ATCAAGCAGC GTTTTATGTT CAACATTGCA 
GATGGTGGCT TCACTGAGCT GCATTCCCTT TGGCAGAATG AGGAACGGGC AGCCACAGTT 
ACAAAGAAGA CCTATGAGAT CTGGCATCGG CGGCATGACT ACTGGCTGCT GGCTGGCATT 
ATTAACCATG GCTATGCTCG GTGGCAGGAC ATTCAGAATG ACCCGCGCTA TGCCATTCTT 
AATGAACCCT TCAAAGGTGA AATGAATCGT GGTAACTTCT TGGAGATAAA AAACAAATTC 
CTGGCTAGAA GATTTAAGCT CTTGGAACAA GCCTTGGTGA TTGAGGAGCA ACTGCGTCGA 
GCAGCTTACC TGAACATGTC AGAGGACCCT TCCCATCCTT CCATGGCCCT CAATACTCGT 
TTTGCTGAAG TGGAATGCCT GGCTGAGAGC CACCAGCACC TGTCTAAGGA GTCAATGGCA 
GGAAATAAGC CAGCTAATGC TGTCCTGCAC AAAGGTATTC TGAAACAACT GGAGGAATTG 
TTGAGTGACA TGAAGGCTGA TGTCACAAGG CTGCCAGCCA CAATTGCCCG TATTCCCCCT 
GTGGCTGTGC GGCTACAGAT GTCAGAGCGC AACATCCTCA GCCGGCTGGC AAACCGGGGC 
CCTGAACCCA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_031938279.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031794139.1 Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

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CloneID	OSa236823
Clone ID Related Accession (Same CDS sequence)	XM_031938279.1
Accession Version	XM_031938279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG GCGAGGAGGA GGTGACTGCT 
GTGGATGGTT ATGAGACAGA CCACCAGGAC TACTGTGAAG TGTGCCAGCA AGGAGGAGAG 
ATCATCCTGT GTGATACCTG TCCCCGTGCT TACCACATGG TCTGCCTGGA TCCTGACATG 
GAGAAGGCCC CGGAGGGCAA ATGGAGTTGT CCACACTGTG AAAAGGAAGG TATCCAGTGG 
GAAGCAAAGG AGGACAACTC TGAGGGTGAA GAAATCCTAG AAGAAGTTGG TGGTGACCCT 
GAGGAGGAGG ATGATCATCA CATGGAGTTT TGCCGTGTCT GCAAGGATGG TGGGGAACTA 
CTCTGCTGTG ATACCTGCCC CTCTTCCTAC CATATCCACT GCTTGAACCC CCCACTCCCA 
GAGATCCCCA ACGGCGAATG GCTTTGTCCT CGTTGCACAT GCCCTTCACT GAAAGGTAAA 
GTTCAGAAGA TCCTGATTTG GAAATGGGGG CAGCCACCAT CCCCTACACC AGTGCCCCGA 
CCCCCTGATG CTGACCCCAA TACCCCTTCT CCTAAGCCTT TAGAGGGGCG ACCTGAGCGG 
CAATTCTTTG TGAAATGGCA AGGCATGTCC TACTGGCACT GTTCTTGGGT GTCAGAACTA 
CAGCTGGAGC TTCACTGCCA AGTGATGTTC CGTAACTACC AGCGTAAGAA TGATATGGAT 
GAGCCACCAT CTGGTGACTT TGGTGGGGAG GAAGAAAAAA GCCGTAAACG AAAGAACAAG 
GACCCCAAGT TTGCAGAAAT GGAAGAACGT TTCTACCGCT ATGGGATTAA ACCTGAGTGG 
ATGATGATCC ACCGTATCCT CAACCACAGT GTAGATAAGA AGGGTCATGT CCACTATTTG 
ATCAAATGGA GGGACCTGCC CTATGATCAG GCATCCTGGG AGAGTGAAGA TGTAGAGGTG 
CAAGACTATG ACCTATTCAA GCAGAGCTAT TGGAACCATA GGGAGCTAAT GCGGGGTGAG 
GAAGGACGAC CTGGCAAAAA GCTCAAGAAG GTTAAGCTTA GGAAGTTGGA GAGGCCTCCT 
GAAACTCCTA CAGTTGATCC AACAGTGAAA TATGAGCGGC AACCAGAGTA CTTAGATGCC 
ACTGGTGGGA CTCTACATCC CTATCAAATG GAGGGATTGA ACTGGTTGCG CTTCTCCTGG 
GCCCAGGGCA CTGACACTAT CTTGGCAGAT GAAATGGGCT TGGGCAAGAC GGTTCAGACC 
GCTGTCTTCC TCTATTCTTT GTACAAGGAG GGTCATTCAA AAGGCCCTTT CCTAGTGAGT 
GCCCCACTTT CTACAATCAT CAACTGGGAG CGAGAGTTTG AGATGTGGGC TCCAGACATG 
TATGTGGTAA CCTATGTTGG AGACAAGGAT AGTCGTGCTA TTATTCGTGA AAACGAGTTC 
TCTTTTGAAG ACAATGCTAT CCGTGGTGGC AAGAAGGCTT CCAGAATGAA GAAAGAAGCA 
TCTGTGAAGT TTCATGTTTT GCTGACTTCC TATGAACTGA TTACCATTGA CATGGCCATT 
CTGGGCTCCA TTGATTGGGC CTGCCTAATT GTGGATGAAG CTCATAGGCT TAAGAACAAT 
CAGTCTAAGT TTTTCAGGGT ACTCAATGGC TATTCCCTCC AGCACAAGTT ACTACTAACA 
GGGACTCCTC TACAGAATAA CTTGGAGGAG CTTTTCCACC TGCTTAATTT CCTCACTCCA 
GAAAGATTCC ACAACCTGGA GGGCTTCCTA GAAGAATTTG CTGACATTGC CAAGGAAGAC 
CAGATCAAAA AATTGCATGA CATGTTGGGG CCTCATATGC TTCGACGGCT TAAAGCTGAT 
GTCTTCAAGA ACATGCCCTC TAAGACAGAG CTGATTGTGC GTGTGGAACT GAGCCCGATG 
CAGAAGAAAT ACTACAAGTA CATTCTAACC CGAAACTTTG AGGCACTCAA TGCTCGGGGT 
GGTGGTAATC AGGTTTCCCT ACTTAATGTG GTGATGGATC TAAAAAAGTG CTGTAACCAC 
CCTTACCTCT TCCCTGTGGC TGCCATGGAA GCACCAAAGA TGCCCAATGG GATGTATGAT 
GGCAGTGCTC TAATTCGAGC ATCAGGGAAG CTACTTTTAT TGCAAAAAAT GCTCAAGAAC 
CTCAAAGAGG GTGGTCACCG TGTGCTCATT TTCTCGCAGA TGACCAAGAT GTTGGATCTG 
TTAGAGGATT TTTTGGAACA TGAAGGTTAT AAATATGAGC GAATCGATGG GGGAATTACT 
GGGAACATGC GTCAGGAAGC CATTGATCGT TTTAACGCAC CTGGGGCTCA GCAGTTTTGC 
TTTCTTCTTT CTACTCGAGC TGGGGGCCTG GGAATCAATC TAGCTACTGC AGATACTGTT 
ATCATCTATG ACTCTGATTG GAACCCCCAT AATGACATCC AGGCTTTTAG CAGAGCCCAT 
CGAATTGGTC AGAATAAGAA AGTGATGATC TACCGTTTTG TGACCCGCGC TTCAGTGGAG 
GAGCGCATCA CACAGGTGGC GAAGAAGAAG ATGATGCTTA CACATCTTGT AGTAAGGCCT 
GGCCTGGGCT CTAAGACTGG CTCCATGTCC AAGCAGGAGC TTGATGATAT CCTCAAGTTT 
GGCACTGAGG AATTATTCAA AGATGAAGCC ACTGATGGAG GAGGAGACAA CAAGGAAGGA 
GAAGACAGTA GTGTTATTCA CTATGATGAC AAGGCCATAG AGCGACTGCT GGATCGAAAT 
CAGGATGAAA CAGAAGACAC AGAACTTCAA GGCATGAATG AATATTTGAG TTCCTTCAAA 
GTGGCCCAGT ATGTAGTACG GGAAGAAGAA ATGGGGGAGG AAGAAGAAGT AGAAAGAGAG 
ATCATCAAGC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAGCTGCT GCGACACCAT 
TATGAGCAGC AGCAAGAGGA TCTGGCCCGA AACCTAGGCA AAGGGAAGCG AATTCGTAAG 
CAGGTCAACT ACAACGATGG CTCCCAGGAG GATCGAGATT GGCAGGATGA CCAGTCAGAC 
AATCAGTCCG ACTACTCAGT GGCCTCAGAG GAAGGCGATG AGGACTTTGA TGAGCGTTCA 
GAAGCTCCCA GACGGCCTAG CCGCAAGGGC CTGCGAAATG ATAAGGACAA ACCATTGCCT 
CCATTGTTGG CTCGTGTTGG TGGAAATATT GAGGTACTTG GCTTCAATGC CCGCCAGCGG 
AAGGCTTTCC TCAATGCCAT TATGCGTTAT GGGATGCCTC CTCAGGATGC CTTTACTACC 
CAATGGCTTG TGCGGGACCT GAGGGGCAAG TCAGAGAAAG AGTTCAAGGC ATATGTGTCT 
CTCTTTATGC GTCACCTTTG TGAACCAGGA GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTACCCCGTG AGGGCTTATC CCGGCAGCAT GTTCTTACTC GTATTGGTGT TATGTCACTT 
ATCCGAAAAA AGGTTCAGGA ATTTGAGCAT GTCAATGGGC GCTGGAGCAT GCCTGAGCTG 
GCTGAGGTAG AAGAGAACAA GAAGATGTCA CAGCCAGGAT CACCTTCACC TAAAACTCCC 
ACACCTTCCA CTCCAGGAGA CACACAACCC AACACTCCTG CACCTGTGCC ACCCCCTGAT 
GATGGGGTGA AAGCAGAGGA GAATAATAAC AAAGAAGAAG AGAACACAGA AGGAGAGAGA 
GAGGTGAAAC CTGCAGCTCC TGAGGCTTCT GTGGAGGCTA CACAGCCCTC TGCATCTAAC 
CCAGAAGAGG AAAAAGTTCT GACTGAGCCT CTGGAAGGAG AAGAGAAGGT GGATAAGCCA 
GAAGTCAAAG AGAAGACAGA GGAGACCATG GAGACAGAAA CCAAAGGTGT TGCTGAAACG 
GAAAAGGTAG AGGAGAAAGC AACAGTGGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAG 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAA GTGATGCTTC AGAATGGGGA GACTCCCAAG 
GACCTGGGTG ATGAGAAGCA GAAGAAGAAC ATCAAGCAGC GTTTTATGTT CAACATTGCA 
GATGGTGGCT TCACTGAGCT GCATTCCCTT TGGCAGAATG AGGAACGGGC AGCCACAGTT 
ACAAAGAAGA CCTATGAGAT CTGGCATCGG CGGCATGACT ACTGGCTGCT GGCTGGCATT 
ATTAACCATG GCTATGCTCG GTGGCAGGAC ATTCAGAATG ACCCGCGCTA TGCCATTCTT 
AATGAACCCT TCAAAGGTGA AATGAATCGT GGTAACTTCT TGGAGATAAA AAACAAATTC 
CTGGCTAGAA GATTTAAGCT CTTGGAACAA GCCTTGGTGA TTGAGGAGCA ACTGCGTCGA 
GCAGCTTACC TGAACATGTC AGAGGACCCT TCCCATCCTT CCATGGCCCT CAATACTCGT 
TTTGCTGAAG TGGAATGCCT GGCTGAGAGC CACCAGCACC TGTCTAAGGA GTCAATGGCA 
GGAAATAAGC CAGCTAATGC TGTCCTGCAC AAAGTTCTGA AACAACTGGA GGAATTGTTG 
AGTGACATGA AGGCTGATGT CACAAGGCTG CCAGCCACAA TTGCCCGTAT TCCCCCTGTG 
GCTGTGCGGC TACAGATGTC AGAGCGCAAC ATCCTCAGCC GGCTGGCAAA CCGGGGCCCT 
GAACCCACCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031794139.1
CDS	219..5954
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERVLLCRQLGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKEPKSSGQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKASPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRATKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRGPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_031938279.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031938279.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG GCGAGGAGGA GGTGACTGCT 
GTGGATGGTT ATGAGACAGA CCACCAGGAC TACTGTGAAG TGTGCCAGCA AGGAGGAGAG 
ATCATCCTGT GTGATACCTG TCCCCGTGCT TACCACATGG TCTGCCTGGA TCCTGACATG 
GAGAAGGCCC CGGAGGGCAA ATGGAGTTGT CCACACTGTG AAAAGGAAGG TATCCAGTGG 
GAAGCAAAGG AGGACAACTC TGAGGGTGAA GAAATCCTAG AAGAAGTTGG TGGTGACCCT 
GAGGAGGAGG ATGATCATCA CATGGAGTTT TGCCGTGTCT GCAAGGATGG TGGGGAACTA 
CTCTGCTGTG ATACCTGCCC CTCTTCCTAC CATATCCACT GCTTGAACCC CCCACTCCCA 
GAGATCCCCA ACGGCGAATG GCTTTGTCCT CGTTGCACAT GCCCTTCACT GAAAGGTAAA 
GTTCAGAAGA TCCTGATTTG GAAATGGGGG CAGCCACCAT CCCCTACACC AGTGCCCCGA 
CCCCCTGATG CTGACCCCAA TACCCCTTCT CCTAAGCCTT TAGAGGGGCG ACCTGAGCGG 
CAATTCTTTG TGAAATGGCA AGGCATGTCC TACTGGCACT GTTCTTGGGT GTCAGAACTA 
CAGCTGGAGC TTCACTGCCA AGTGATGTTC CGTAACTACC AGCGTAAGAA TGATATGGAT 
GAGCCACCAT CTGGTGACTT TGGTGGGGAG GAAGAAAAAA GCCGTAAACG AAAGAACAAG 
GACCCCAAGT TTGCAGAAAT GGAAGAACGT TTCTACCGCT ATGGGATTAA ACCTGAGTGG 
ATGATGATCC ACCGTATCCT CAACCACAGT GTAGATAAGA AGGGTCATGT CCACTATTTG 
ATCAAATGGA GGGACCTGCC CTATGATCAG GCATCCTGGG AGAGTGAAGA TGTAGAGGTG 
CAAGACTATG ACCTATTCAA GCAGAGCTAT TGGAACCATA GGGAGCTAAT GCGGGGTGAG 
GAAGGACGAC CTGGCAAAAA GCTCAAGAAG GTTAAGCTTA GGAAGTTGGA GAGGCCTCCT 
GAAACTCCTA CAGTTGATCC AACAGTGAAA TATGAGCGGC AACCAGAGTA CTTAGATGCC 
ACTGGTGGGA CTCTACATCC CTATCAAATG GAGGGATTGA ACTGGTTGCG CTTCTCCTGG 
GCCCAGGGCA CTGACACTAT CTTGGCAGAT GAAATGGGCT TGGGCAAGAC GGTTCAGACC 
GCTGTCTTCC TCTATTCTTT GTACAAGGAG GGTCATTCAA AAGGCCCTTT CCTAGTGAGT 
GCCCCACTTT CTACAATCAT CAACTGGGAG CGAGAGTTTG AGATGTGGGC TCCAGACATG 
TATGTGGTAA CCTATGTTGG AGACAAGGAT AGTCGTGCTA TTATTCGTGA AAACGAGTTC 
TCTTTTGAAG ACAATGCTAT CCGTGGTGGC AAGAAGGCTT CCAGAATGAA GAAAGAAGCA 
TCTGTGAAGT TTCATGTTTT GCTGACTTCC TATGAACTGA TTACCATTGA CATGGCCATT 
CTGGGCTCCA TTGATTGGGC CTGCCTAATT GTGGATGAAG CTCATAGGCT TAAGAACAAT 
CAGTCTAAGT TTTTCAGGGT ACTCAATGGC TATTCCCTCC AGCACAAGTT ACTACTAACA 
GGGACTCCTC TACAGAATAA CTTGGAGGAG CTTTTCCACC TGCTTAATTT CCTCACTCCA 
GAAAGATTCC ACAACCTGGA GGGCTTCCTA GAAGAATTTG CTGACATTGC CAAGGAAGAC 
CAGATCAAAA AATTGCATGA CATGTTGGGG CCTCATATGC TTCGACGGCT TAAAGCTGAT 
GTCTTCAAGA ACATGCCCTC TAAGACAGAG CTGATTGTGC GTGTGGAACT GAGCCCGATG 
CAGAAGAAAT ACTACAAGTA CATTCTAACC CGAAACTTTG AGGCACTCAA TGCTCGGGGT 
GGTGGTAATC AGGTTTCCCT ACTTAATGTG GTGATGGATC TAAAAAAGTG CTGTAACCAC 
CCTTACCTCT TCCCTGTGGC TGCCATGGAA GCACCAAAGA TGCCCAATGG GATGTATGAT 
GGCAGTGCTC TAATTCGAGC ATCAGGGAAG CTACTTTTAT TGCAAAAAAT GCTCAAGAAC 
CTCAAAGAGG GTGGTCACCG TGTGCTCATT TTCTCGCAGA TGACCAAGAT GTTGGATCTG 
TTAGAGGATT TTTTGGAACA TGAAGGTTAT AAATATGAGC GAATCGATGG GGGAATTACT 
GGGAACATGC GTCAGGAAGC CATTGATCGT TTTAACGCAC CTGGGGCTCA GCAGTTTTGC 
TTTCTTCTTT CTACTCGAGC TGGGGGCCTG GGAATCAATC TAGCTACTGC AGATACTGTT 
ATCATCTATG ACTCTGATTG GAACCCCCAT AATGACATCC AGGCTTTTAG CAGAGCCCAT 
CGAATTGGTC AGAATAAGAA AGTGATGATC TACCGTTTTG TGACCCGCGC TTCAGTGGAG 
GAGCGCATCA CACAGGTGGC GAAGAAGAAG ATGATGCTTA CACATCTTGT AGTAAGGCCT 
GGCCTGGGCT CTAAGACTGG CTCCATGTCC AAGCAGGAGC TTGATGATAT CCTCAAGTTT 
GGCACTGAGG AATTATTCAA AGATGAAGCC ACTGATGGAG GAGGAGACAA CAAGGAAGGA 
GAAGACAGTA GTGTTATTCA CTATGATGAC AAGGCCATAG AGCGACTGCT GGATCGAAAT 
CAGGATGAAA CAGAAGACAC AGAACTTCAA GGCATGAATG AATATTTGAG TTCCTTCAAA 
GTGGCCCAGT ATGTAGTACG GGAAGAAGAA ATGGGGGAGG AAGAAGAAGT AGAAAGAGAG 
ATCATCAAGC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAGCTGCT GCGACACCAT 
TATGAGCAGC AGCAAGAGGA TCTGGCCCGA AACCTAGGCA AAGGGAAGCG AATTCGTAAG 
CAGGTCAACT ACAACGATGG CTCCCAGGAG GATCGAGATT GGCAGGATGA CCAGTCAGAC 
AATCAGTCCG ACTACTCAGT GGCCTCAGAG GAAGGCGATG AGGACTTTGA TGAGCGTTCA 
GAAGCTCCCA GACGGCCTAG CCGCAAGGGC CTGCGAAATG ATAAGGACAA ACCATTGCCT 
CCATTGTTGG CTCGTGTTGG TGGAAATATT GAGGTACTTG GCTTCAATGC CCGCCAGCGG 
AAGGCTTTCC TCAATGCCAT TATGCGTTAT GGGATGCCTC CTCAGGATGC CTTTACTACC 
CAATGGCTTG TGCGGGACCT GAGGGGCAAG TCAGAGAAAG AGTTCAAGGC ATATGTGTCT 
CTCTTTATGC GTCACCTTTG TGAACCAGGA GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTACCCCGTG AGGGCTTATC CCGGCAGCAT GTTCTTACTC GTATTGGTGT TATGTCACTT 
ATCCGAAAAA AGGTTCAGGA ATTTGAGCAT GTCAATGGGC GCTGGAGCAT GCCTGAGCTG 
GCTGAGGTAG AAGAGAACAA GAAGATGTCA CAGCCAGGAT CACCTTCACC TAAAACTCCC 
ACACCTTCCA CTCCAGGAGA CACACAACCC AACACTCCTG CACCTGTGCC ACCCCCTGAT 
GATGGGGTGA AAGCAGAGGA GAATAATAAC AAAGAAGAAG AGAACACAGA AGGAGAGAGA 
GAGGTGAAAC CTGCAGCTCC TGAGGCTTCT GTGGAGGCTA CACAGCCCTC TGCATCTAAC 
CCAGAAGAGG AAAAAGTTCT GACTGAGCCT CTGGAAGGAG AAGAGAAGGT GGATAAGCCA 
GAAGTCAAAG AGAAGACAGA GGAGACCATG GAGACAGAAA CCAAAGGTGT TGCTGAAACG 
GAAAAGGTAG AGGAGAAAGC AACAGTGGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAG 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAA GTGATGCTTC AGAATGGGGA GACTCCCAAG 
GACCTGGGTG ATGAGAAGCA GAAGAAGAAC ATCAAGCAGC GTTTTATGTT CAACATTGCA 
GATGGTGGCT TCACTGAGCT GCATTCCCTT TGGCAGAATG AGGAACGGGC AGCCACAGTT 
ACAAAGAAGA CCTATGAGAT CTGGCATCGG CGGCATGACT ACTGGCTGCT GGCTGGCATT 
ATTAACCATG GCTATGCTCG GTGGCAGGAC ATTCAGAATG ACCCGCGCTA TGCCATTCTT 
AATGAACCCT TCAAAGGTGA AATGAATCGT GGTAACTTCT TGGAGATAAA AAACAAATTC 
CTGGCTAGAA GATTTAAGCT CTTGGAACAA GCCTTGGTGA TTGAGGAGCA ACTGCGTCGA 
GCAGCTTACC TGAACATGTC AGAGGACCCT TCCCATCCTT CCATGGCCCT CAATACTCGT 
TTTGCTGAAG TGGAATGCCT GGCTGAGAGC CACCAGCACC TGTCTAAGGA GTCAATGGCA 
GGAAATAAGC CAGCTAATGC TGTCCTGCAC AAAGTTCTGA AACAACTGGA GGAATTGTTG 
AGTGACATGA AGGCTGATGT CACAAGGCTG CCAGCCACAA TTGCCCGTAT TCCCCCTGTG 
GCTGTGCGGC TACAGATGTC AGAGCGCAAC ATCCTCAGCC GGCTGGCAAA CCGGGGCCCT 
GAACCCACCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_031938280.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031794140.1 Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

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CloneID	OSa236824
Clone ID Related Accession (Same CDS sequence)	XM_031938280.1
Accession Version	XM_031938280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC TGGGAGACAG TTCTGGGGAG 
GGGCCTGAAT TTGTGGAGGA AGAAGATGAG ATGGTTTTGC GATCAGACAG CGAGGGCAGT 
GACTATACCC CTGGCAAGAA AAAGAAGAAG AAGCTTGGAC CTAAGAAGGA GAAGAAAAGC 
AAGTCTAAGA GAAAGGAGGA AGAAGAAGAA GATGATGATG ATGATGACTC AAAGGAGCCC 
AAGTCATCAG GTCAGCTACT AGAAGATTGG GGCATGGAGG ACATTGACCA TGTGTTCTCA 
GAAGAAGATT ATCGAACTCT TACCAACTAT AAGGCCTTCA GCCAGTTTGT CAGACCTCTC 
ATTGCTGCTA AGAACCCCAA GATTGCAGTT TCCAAGATGA TGATGGTTCT AGGAGCCAAA 
TGGCGAGAAT TCAGTACCAA CAACCCCTTC AAGGGCAGTT CCGGGGCCTC AGTGGCAGCT 
GCAGCTGCGG CAGCAGTAGC TGTAGTGGAG AGTATGGTAA CTGCAACTGA AGTTGCTCCA 
CCTCCACCCC CTGTGGAAGT ACCCATCAGA AAGGCCAAAA CCAAGGAGGG TAAAGGTCCC 
AATGCCCGAC GGAAACCTAA AGCTAGCCCC CGTGTGCCTG ATGCTAAGAA GCCCAAACCG 
AAGAAGGTGG CACCCCTGAA AATCAAACTG GGAGGCTTTG GCTCTAAGCG CAAGAGGTCT 
TCGAGTGAAG ATGATGACCT GGATGTGGAA TCTGACTTTG ATGATGCCAG TATCAACAGT 
TACTCCGTGT CAGATGGTTC GACTAGTAGG AGCAGCCGCA GCCGCAAGAA ACTTAGAGCT 
ACCAAAAAGA AAAAGAAAGG CGAGGAGGAG GTGACTGCTG TGGATGGTTA TGAGACAGAC 
CACCAGGACT ACTGTGAAGT GTGCCAGCAA GGAGGAGAGA TCATCCTGTG TGATACCTGT 
CCCCGTGCTT ACCACATGGT CTGCCTGGAT CCTGACATGG AGAAGGCCCC GGAGGGCAAA 
TGGAGTTGTC CACACTGTGA AAAGGAAGGT ATCCAGTGGG AAGCAAAGGA GGACAACTCT 
GAGGGTGAAG AAATCCTAGA AGAAGTTGGT GGTGACCCTG AGGAGGAGGA TGATCATCAC 
ATGGAGTTTT GCCGTGTCTG CAAGGATGGT GGGGAACTAC TCTGCTGTGA TACCTGCCCC 
TCTTCCTACC ATATCCACTG CTTGAACCCC CCACTCCCAG AGATCCCCAA CGGCGAATGG 
CTTTGTCCTC GTTGCACATG CCCTTCACTG AAAGGTAAAG TTCAGAAGAT CCTGATTTGG 
AAATGGGGGC AGCCACCATC CCCTACACCA GTGCCCCGAC CCCCTGATGC TGACCCCAAT 
ACCCCTTCTC CTAAGCCTTT AGAGGGGCGA CCTGAGCGGC AATTCTTTGT GAAATGGCAA 
GGCATGTCCT ACTGGCACTG TTCTTGGGTG TCAGAACTAC AGCTGGAGCT TCACTGCCAA 
GTGATGTTCC GTAACTACCA GCGTAAGAAT GATATGGATG AGCCACCATC TGGTGACTTT 
GGTGGGGAGG AAGAAAAAAG CCGTAAACGA AAGAACAAGG ACCCCAAGTT TGCAGAAATG 
GAAGAACGTT TCTACCGCTA TGGGATTAAA CCTGAGTGGA TGATGATCCA CCGTATCCTC 
AACCACAGTG TAGATAAGAA GGGTCATGTC CACTATTTGA TCAAATGGAG GGACCTGCCC 
TATGATCAGG CATCCTGGGA GAGTGAAGAT GTAGAGGTGC AAGACTATGA CCTATTCAAG 
CAGAGCTATT GGAACCATAG GGAGCTAATG CGGGGTGAGG AAGGACGACC TGGCAAAAAG 
CTCAAGAAGG TTAAGCTTAG GAAGTTGGAG AGGCCTCCTG AAACTCCTAC AGTTGATCCA 
ACAGTGAAAT ATGAGCGGCA ACCAGAGTAC TTAGATGCCA CTGGTGGGAC TCTACATCCC 
TATCAAATGG AGGGATTGAA CTGGTTGCGC TTCTCCTGGG CCCAGGGCAC TGACACTATC 
TTGGCAGATG AAATGGGCTT GGGCAAGACG GTTCAGACCG CTGTCTTCCT CTATTCTTTG 
TACAAGGAGG GTCATTCAAA AGGCCCTTTC CTAGTGAGTG CCCCACTTTC TACAATCATC 
AACTGGGAGC GAGAGTTTGA GATGTGGGCT CCAGACATGT ATGTGGTAAC CTATGTTGGA 
GACAAGGATA GTCGTGCTAT TATTCGTGAA AACGAGTTCT CTTTTGAAGA CAATGCTATC 
CGTGGTGGCA AGAAGGCTTC CAGAATGAAG AAAGAAGCAT CTGTGAAGTT TCATGTTTTG 
CTGACTTCCT ATGAACTGAT TACCATTGAC ATGGCCATTC TGGGCTCCAT TGATTGGGCC 
TGCCTAATTG TGGATGAAGC TCATAGGCTT AAGAACAATC AGTCTAAGTT TTTCAGGGTA 
CTCAATGGCT ATTCCCTCCA GCACAAGTTA CTACTAACAG GGACTCCTCT ACAGAATAAC 
TTGGAGGAGC TTTTCCACCT GCTTAATTTC CTCACTCCAG AAAGATTCCA CAACCTGGAG 
GGCTTCCTAG AAGAATTTGC TGACATTGCC AAGGAAGACC AGATCAAAAA ATTGCATGAC 
ATGTTGGGGC CTCATATGCT TCGACGGCTT AAAGCTGATG TCTTCAAGAA CATGCCCTCT 
AAGACAGAGC TGATTGTGCG TGTGGAACTG AGCCCGATGC AGAAGAAATA CTACAAGTAC 
ATTCTAACCC GAAACTTTGA GGCACTCAAT GCTCGGGGTG GTGGTAATCA GGTTTCCCTA 
CTTAATGTGG TGATGGATCT AAAAAAGTGC TGTAACCACC CTTACCTCTT CCCTGTGGCT 
GCCATGGAAG CACCAAAGAT GCCCAATGGG ATGTATGATG GCAGTGCTCT AATTCGAGCA 
TCAGGGAAGC TACTTTTATT GCAAAAAATG CTCAAGAACC TCAAAGAGGG TGGTCACCGT 
GTGCTCATTT TCTCGCAGAT GACCAAGATG TTGGATCTGT TAGAGGATTT TTTGGAACAT 
GAAGGTTATA AATATGAGCG AATCGATGGG GGAATTACTG GGAACATGCG TCAGGAAGCC 
ATTGATCGTT TTAACGCACC TGGGGCTCAG CAGTTTTGCT TTCTTCTTTC TACTCGAGCT 
GGGGGCCTGG GAATCAATCT AGCTACTGCA GATACTGTTA TCATCTATGA CTCTGATTGG 
AACCCCCATA ATGACATCCA GGCTTTTAGC AGAGCCCATC GAATTGGTCA GAATAAGAAA 
GTGATGATCT ACCGTTTTGT GACCCGCGCT TCAGTGGAGG AGCGCATCAC ACAGGTGGCG 
AAGAAGAAGA TGATGCTTAC ACATCTTGTA GTAAGGCCTG GCCTGGGCTC TAAGACTGGC 
TCCATGTCCA AGCAGGAGCT TGATGATATC CTCAAGTTTG GCACTGAGGA ATTATTCAAA 
GATGAAGCCA CTGATGGAGG AGGAGACAAC AAGGAAGGAG AAGACAGTAG TGTTATTCAC 
TATGATGACA AGGCCATAGA GCGACTGCTG GATCGAAATC AGGATGAAAC AGAAGACACA 
GAACTTCAAG GCATGAATGA ATATTTGAGT TCCTTCAAAG TGGCCCAGTA TGTAGTACGG 
GAAGAAGAAA TGGGGGAGGA AGAAGAAGTA GAAAGAGAGA TCATCAAGCA GGAAGAAAGT 
GTGGATCCTG ACTACTGGGA GAAGCTGCTG CGACACCATT ATGAGCAGCA GCAAGAGGAT 
CTGGCCCGAA ACCTAGGCAA AGGGAAGCGA ATTCGTAAGC AGGTCAACTA CAACGATGGC 
TCCCAGGAGG ATCGAGATTG GCAGGATGAC CAGTCAGACA ATCAGTCCGA CTACTCAGTG 
GCCTCAGAGG AAGGCGATGA GGACTTTGAT GAGCGTTCAG AAGCTCCCAG ACGGCCTAGC 
CGCAAGGGCC TGCGAAATGA TAAGGACAAA CCATTGCCTC CATTGTTGGC TCGTGTTGGT 
GGAAATATTG AGGTACTTGG CTTCAATGCC CGCCAGCGGA AGGCTTTCCT CAATGCCATT 
ATGCGTTATG GGATGCCTCC TCAGGATGCC TTTACTACCC AATGGCTTGT GCGGGACCTG 
AGGGGCAAGT CAGAGAAAGA GTTCAAGGCA TATGTGTCTC TCTTTATGCG TCACCTTTGT 
GAACCAGGAG CAGATGGGGC TGAGACCTTT GCTGATGGTG TACCCCGTGA GGGCTTATCC 
CGGCAGCATG TTCTTACTCG TATTGGTGTT ATGTCACTTA TCCGAAAAAA GGTTCAGGAA 
TTTGAGCATG TCAATGGGCG CTGGAGCATG CCTGAGCTGG CTGAGGTAGA AGAGAACAAG 
AAGATGTCAC AGCCAGGATC ACCTTCACCT AAAACTCCCA CACCTTCCAC TCCAGGAGAC 
ACACAACCCA ACACTCCTGC ACCTGTGCCA CCCCCTGATG ATGGGGTGAA AGCAGAGGAG 
AATAATAACA AAGAAGAAGA GAACACAGAA GGAGAGAGAG AGGTGAAACC TGCAGCTCCT 
GAGGCTTCTG TGGAGGCTAC ACAGCCCTCT GCATCTAACC CAGAAGAGGA AAAAGTTCTG 
ACTGAGCCTC TGGAAGGAGA AGAGAAGGTG GATAAGCCAG AAGTCAAAGA GAAGACAGAG 
GAGACCATGG AGACAGAAAC CAAAGGTGTT GCTGAAACGG AAAAGGTAGA GGAGAAAGCA 
ACAGTGGATC TGACCCCTAT TGTGGTAGAA GACAAAGAGG AGAAGAAAGA AGAAGAAGAG 
AAAAAAGAAG TGATGCTTCA GAATGGGGAG ACTCCCAAGG ACCTGGGTGA TGAGAAGCAG 
AAGAAGAACA TCAAGCAGCG TTTTATGTTC AACATTGCAG ATGGTGGCTT CACTGAGCTG 
CATTCCCTTT GGCAGAATGA GGAACGGGCA GCCACAGTTA CAAAGAAGAC CTATGAGATC 
TGGCATCGGC GGCATGACTA CTGGCTGCTG GCTGGCATTA TTAACCATGG CTATGCTCGG 
TGGCAGGACA TTCAGAATGA CCCGCGCTAT GCCATTCTTA ATGAACCCTT CAAAGGTGAA 
ATGAATCGTG GTAACTTCTT GGAGATAAAA AACAAATTCC TGGCTAGAAG ATTTAAGCTC 
TTGGAACAAG CCTTGGTGAT TGAGGAGCAA CTGCGTCGAG CAGCTTACCT GAACATGTCA 
GAGGACCCTT CCCATCCTTC CATGGCCCTC AATACTCGTT TTGCTGAAGT GGAATGCCTG 
GCTGAGAGCC ACCAGCACCT GTCTAAGGAG TCAATGGCAG GAAATAAGCC AGCTAATGCT 
GTCCTGCACA AAGGTATTCT GAAACAACTG GAGGAATTGT TGAGTGACAT GAAGGCTGAT 
GTCACAAGGC TGCCAGCCAC AATTGCCCGT ATTCCCCCTG TGGCTGTGCG GCTACAGATG 
TCAGAGCGCA ACATCCTCAG CCGGCTGGCA AACCGGGGCC CTGAACCCAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031794140.1
CDS	220..5937
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKEPKSSGQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKASPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRATKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRGPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_031938280.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031938280.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC TGGGAGACAG TTCTGGGGAG 
GGGCCTGAAT TTGTGGAGGA AGAAGATGAG ATGGTTTTGC GATCAGACAG CGAGGGCAGT 
GACTATACCC CTGGCAAGAA AAAGAAGAAG AAGCTTGGAC CTAAGAAGGA GAAGAAAAGC 
AAGTCTAAGA GAAAGGAGGA AGAAGAAGAA GATGATGATG ATGATGACTC AAAGGAGCCC 
AAGTCATCAG GTCAGCTACT AGAAGATTGG GGCATGGAGG ACATTGACCA TGTGTTCTCA 
GAAGAAGATT ATCGAACTCT TACCAACTAT AAGGCCTTCA GCCAGTTTGT CAGACCTCTC 
ATTGCTGCTA AGAACCCCAA GATTGCAGTT TCCAAGATGA TGATGGTTCT AGGAGCCAAA 
TGGCGAGAAT TCAGTACCAA CAACCCCTTC AAGGGCAGTT CCGGGGCCTC AGTGGCAGCT 
GCAGCTGCGG CAGCAGTAGC TGTAGTGGAG AGTATGGTAA CTGCAACTGA AGTTGCTCCA 
CCTCCACCCC CTGTGGAAGT ACCCATCAGA AAGGCCAAAA CCAAGGAGGG TAAAGGTCCC 
AATGCCCGAC GGAAACCTAA AGCTAGCCCC CGTGTGCCTG ATGCTAAGAA GCCCAAACCG 
AAGAAGGTGG CACCCCTGAA AATCAAACTG GGAGGCTTTG GCTCTAAGCG CAAGAGGTCT 
TCGAGTGAAG ATGATGACCT GGATGTGGAA TCTGACTTTG ATGATGCCAG TATCAACAGT 
TACTCCGTGT CAGATGGTTC GACTAGTAGG AGCAGCCGCA GCCGCAAGAA ACTTAGAGCT 
ACCAAAAAGA AAAAGAAAGG CGAGGAGGAG GTGACTGCTG TGGATGGTTA TGAGACAGAC 
CACCAGGACT ACTGTGAAGT GTGCCAGCAA GGAGGAGAGA TCATCCTGTG TGATACCTGT 
CCCCGTGCTT ACCACATGGT CTGCCTGGAT CCTGACATGG AGAAGGCCCC GGAGGGCAAA 
TGGAGTTGTC CACACTGTGA AAAGGAAGGT ATCCAGTGGG AAGCAAAGGA GGACAACTCT 
GAGGGTGAAG AAATCCTAGA AGAAGTTGGT GGTGACCCTG AGGAGGAGGA TGATCATCAC 
ATGGAGTTTT GCCGTGTCTG CAAGGATGGT GGGGAACTAC TCTGCTGTGA TACCTGCCCC 
TCTTCCTACC ATATCCACTG CTTGAACCCC CCACTCCCAG AGATCCCCAA CGGCGAATGG 
CTTTGTCCTC GTTGCACATG CCCTTCACTG AAAGGTAAAG TTCAGAAGAT CCTGATTTGG 
AAATGGGGGC AGCCACCATC CCCTACACCA GTGCCCCGAC CCCCTGATGC TGACCCCAAT 
ACCCCTTCTC CTAAGCCTTT AGAGGGGCGA CCTGAGCGGC AATTCTTTGT GAAATGGCAA 
GGCATGTCCT ACTGGCACTG TTCTTGGGTG TCAGAACTAC AGCTGGAGCT TCACTGCCAA 
GTGATGTTCC GTAACTACCA GCGTAAGAAT GATATGGATG AGCCACCATC TGGTGACTTT 
GGTGGGGAGG AAGAAAAAAG CCGTAAACGA AAGAACAAGG ACCCCAAGTT TGCAGAAATG 
GAAGAACGTT TCTACCGCTA TGGGATTAAA CCTGAGTGGA TGATGATCCA CCGTATCCTC 
AACCACAGTG TAGATAAGAA GGGTCATGTC CACTATTTGA TCAAATGGAG GGACCTGCCC 
TATGATCAGG CATCCTGGGA GAGTGAAGAT GTAGAGGTGC AAGACTATGA CCTATTCAAG 
CAGAGCTATT GGAACCATAG GGAGCTAATG CGGGGTGAGG AAGGACGACC TGGCAAAAAG 
CTCAAGAAGG TTAAGCTTAG GAAGTTGGAG AGGCCTCCTG AAACTCCTAC AGTTGATCCA 
ACAGTGAAAT ATGAGCGGCA ACCAGAGTAC TTAGATGCCA CTGGTGGGAC TCTACATCCC 
TATCAAATGG AGGGATTGAA CTGGTTGCGC TTCTCCTGGG CCCAGGGCAC TGACACTATC 
TTGGCAGATG AAATGGGCTT GGGCAAGACG GTTCAGACCG CTGTCTTCCT CTATTCTTTG 
TACAAGGAGG GTCATTCAAA AGGCCCTTTC CTAGTGAGTG CCCCACTTTC TACAATCATC 
AACTGGGAGC GAGAGTTTGA GATGTGGGCT CCAGACATGT ATGTGGTAAC CTATGTTGGA 
GACAAGGATA GTCGTGCTAT TATTCGTGAA AACGAGTTCT CTTTTGAAGA CAATGCTATC 
CGTGGTGGCA AGAAGGCTTC CAGAATGAAG AAAGAAGCAT CTGTGAAGTT TCATGTTTTG 
CTGACTTCCT ATGAACTGAT TACCATTGAC ATGGCCATTC TGGGCTCCAT TGATTGGGCC 
TGCCTAATTG TGGATGAAGC TCATAGGCTT AAGAACAATC AGTCTAAGTT TTTCAGGGTA 
CTCAATGGCT ATTCCCTCCA GCACAAGTTA CTACTAACAG GGACTCCTCT ACAGAATAAC 
TTGGAGGAGC TTTTCCACCT GCTTAATTTC CTCACTCCAG AAAGATTCCA CAACCTGGAG 
GGCTTCCTAG AAGAATTTGC TGACATTGCC AAGGAAGACC AGATCAAAAA ATTGCATGAC 
ATGTTGGGGC CTCATATGCT TCGACGGCTT AAAGCTGATG TCTTCAAGAA CATGCCCTCT 
AAGACAGAGC TGATTGTGCG TGTGGAACTG AGCCCGATGC AGAAGAAATA CTACAAGTAC 
ATTCTAACCC GAAACTTTGA GGCACTCAAT GCTCGGGGTG GTGGTAATCA GGTTTCCCTA 
CTTAATGTGG TGATGGATCT AAAAAAGTGC TGTAACCACC CTTACCTCTT CCCTGTGGCT 
GCCATGGAAG CACCAAAGAT GCCCAATGGG ATGTATGATG GCAGTGCTCT AATTCGAGCA 
TCAGGGAAGC TACTTTTATT GCAAAAAATG CTCAAGAACC TCAAAGAGGG TGGTCACCGT 
GTGCTCATTT TCTCGCAGAT GACCAAGATG TTGGATCTGT TAGAGGATTT TTTGGAACAT 
GAAGGTTATA AATATGAGCG AATCGATGGG GGAATTACTG GGAACATGCG TCAGGAAGCC 
ATTGATCGTT TTAACGCACC TGGGGCTCAG CAGTTTTGCT TTCTTCTTTC TACTCGAGCT 
GGGGGCCTGG GAATCAATCT AGCTACTGCA GATACTGTTA TCATCTATGA CTCTGATTGG 
AACCCCCATA ATGACATCCA GGCTTTTAGC AGAGCCCATC GAATTGGTCA GAATAAGAAA 
GTGATGATCT ACCGTTTTGT GACCCGCGCT TCAGTGGAGG AGCGCATCAC ACAGGTGGCG 
AAGAAGAAGA TGATGCTTAC ACATCTTGTA GTAAGGCCTG GCCTGGGCTC TAAGACTGGC 
TCCATGTCCA AGCAGGAGCT TGATGATATC CTCAAGTTTG GCACTGAGGA ATTATTCAAA 
GATGAAGCCA CTGATGGAGG AGGAGACAAC AAGGAAGGAG AAGACAGTAG TGTTATTCAC 
TATGATGACA AGGCCATAGA GCGACTGCTG GATCGAAATC AGGATGAAAC AGAAGACACA 
GAACTTCAAG GCATGAATGA ATATTTGAGT TCCTTCAAAG TGGCCCAGTA TGTAGTACGG 
GAAGAAGAAA TGGGGGAGGA AGAAGAAGTA GAAAGAGAGA TCATCAAGCA GGAAGAAAGT 
GTGGATCCTG ACTACTGGGA GAAGCTGCTG CGACACCATT ATGAGCAGCA GCAAGAGGAT 
CTGGCCCGAA ACCTAGGCAA AGGGAAGCGA ATTCGTAAGC AGGTCAACTA CAACGATGGC 
TCCCAGGAGG ATCGAGATTG GCAGGATGAC CAGTCAGACA ATCAGTCCGA CTACTCAGTG 
GCCTCAGAGG AAGGCGATGA GGACTTTGAT GAGCGTTCAG AAGCTCCCAG ACGGCCTAGC 
CGCAAGGGCC TGCGAAATGA TAAGGACAAA CCATTGCCTC CATTGTTGGC TCGTGTTGGT 
GGAAATATTG AGGTACTTGG CTTCAATGCC CGCCAGCGGA AGGCTTTCCT CAATGCCATT 
ATGCGTTATG GGATGCCTCC TCAGGATGCC TTTACTACCC AATGGCTTGT GCGGGACCTG 
AGGGGCAAGT CAGAGAAAGA GTTCAAGGCA TATGTGTCTC TCTTTATGCG TCACCTTTGT 
GAACCAGGAG CAGATGGGGC TGAGACCTTT GCTGATGGTG TACCCCGTGA GGGCTTATCC 
CGGCAGCATG TTCTTACTCG TATTGGTGTT ATGTCACTTA TCCGAAAAAA GGTTCAGGAA 
TTTGAGCATG TCAATGGGCG CTGGAGCATG CCTGAGCTGG CTGAGGTAGA AGAGAACAAG 
AAGATGTCAC AGCCAGGATC ACCTTCACCT AAAACTCCCA CACCTTCCAC TCCAGGAGAC 
ACACAACCCA ACACTCCTGC ACCTGTGCCA CCCCCTGATG ATGGGGTGAA AGCAGAGGAG 
AATAATAACA AAGAAGAAGA GAACACAGAA GGAGAGAGAG AGGTGAAACC TGCAGCTCCT 
GAGGCTTCTG TGGAGGCTAC ACAGCCCTCT GCATCTAACC CAGAAGAGGA AAAAGTTCTG 
ACTGAGCCTC TGGAAGGAGA AGAGAAGGTG GATAAGCCAG AAGTCAAAGA GAAGACAGAG 
GAGACCATGG AGACAGAAAC CAAAGGTGTT GCTGAAACGG AAAAGGTAGA GGAGAAAGCA 
ACAGTGGATC TGACCCCTAT TGTGGTAGAA GACAAAGAGG AGAAGAAAGA AGAAGAAGAG 
AAAAAAGAAG TGATGCTTCA GAATGGGGAG ACTCCCAAGG ACCTGGGTGA TGAGAAGCAG 
AAGAAGAACA TCAAGCAGCG TTTTATGTTC AACATTGCAG ATGGTGGCTT CACTGAGCTG 
CATTCCCTTT GGCAGAATGA GGAACGGGCA GCCACAGTTA CAAAGAAGAC CTATGAGATC 
TGGCATCGGC GGCATGACTA CTGGCTGCTG GCTGGCATTA TTAACCATGG CTATGCTCGG 
TGGCAGGACA TTCAGAATGA CCCGCGCTAT GCCATTCTTA ATGAACCCTT CAAAGGTGAA 
ATGAATCGTG GTAACTTCTT GGAGATAAAA AACAAATTCC TGGCTAGAAG ATTTAAGCTC 
TTGGAACAAG CCTTGGTGAT TGAGGAGCAA CTGCGTCGAG CAGCTTACCT GAACATGTCA 
GAGGACCCTT CCCATCCTTC CATGGCCCTC AATACTCGTT TTGCTGAAGT GGAATGCCTG 
GCTGAGAGCC ACCAGCACCT GTCTAAGGAG TCAATGGCAG GAAATAAGCC AGCTAATGCT 
GTCCTGCACA AAGGTATTCT GAAACAACTG GAGGAATTGT TGAGTGACAT GAAGGCTGAT 
GTCACAAGGC TGCCAGCCAC AATTGCCCGT ATTCCCCCTG TGGCTGTGCG GCTACAGATG 
TCAGAGCGCA ACATCCTCAG CCGGCTGGCA AACCGGGGCC CTGAACCCAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_031938281.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031794141.1 Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.

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CloneID	OSa236825
Clone ID Related Accession (Same CDS sequence)	XM_031938281.1
Accession Version	XM_031938281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC TGGGAGACAG TTCTGGGGAG 
GGGCCTGAAT TTGTGGAGGA AGAAGATGAG ATGGTTTTGC GATCAGACAG CGAGGGCAGT 
GACTATACCC CTGGCAAGAA AAAGAAGAAG AAGCTTGGAC CTAAGAAGGA GAAGAAAAGC 
AAGTCTAAGA GAAAGGAGGA AGAAGAAGAA GATGATGATG ATGATGACTC AAAGGAGCCC 
AAGTCATCAG GTCAGCTACT AGAAGATTGG GGCATGGAGG ACATTGACCA TGTGTTCTCA 
GAAGAAGATT ATCGAACTCT TACCAACTAT AAGGCCTTCA GCCAGTTTGT CAGACCTCTC 
ATTGCTGCTA AGAACCCCAA GATTGCAGTT TCCAAGATGA TGATGGTTCT AGGAGCCAAA 
TGGCGAGAAT TCAGTACCAA CAACCCCTTC AAGGGCAGTT CCGGGGCCTC AGTGGCAGCT 
GCAGCTGCGG CAGCAGTAGC TGTAGTGGAG AGTATGGTAA CTGCAACTGA AGTTGCTCCA 
CCTCCACCCC CTGTGGAAGT ACCCATCAGA AAGGCCAAAA CCAAGGAGGG TAAAGGTCCC 
AATGCCCGAC GGAAACCTAA AGCTAGCCCC CGTGTGCCTG ATGCTAAGAA GCCCAAACCG 
AAGAAGGTGG CACCCCTGAA AATCAAACTG GGAGGCTTTG GCTCTAAGCG CAAGAGGTCT 
TCGAGTGAAG ATGATGACCT GGATGTGGAA TCTGACTTTG ATGATGCCAG TATCAACAGT 
TACTCCGTGT CAGATGGTTC GACTAGTAGG AGCAGCCGCA GCCGCAAGAA ACTTAGAGCT 
ACCAAAAAGA AAAAGAAAGG CGAGGAGGAG GTGACTGCTG TGGATGGTTA TGAGACAGAC 
CACCAGGACT ACTGTGAAGT GTGCCAGCAA GGAGGAGAGA TCATCCTGTG TGATACCTGT 
CCCCGTGCTT ACCACATGGT CTGCCTGGAT CCTGACATGG AGAAGGCCCC GGAGGGCAAA 
TGGAGTTGTC CACACTGTGA AAAGGAAGGT ATCCAGTGGG AAGCAAAGGA GGACAACTCT 
GAGGGTGAAG AAATCCTAGA AGAAGTTGGT GGTGACCCTG AGGAGGAGGA TGATCATCAC 
ATGGAGTTTT GCCGTGTCTG CAAGGATGGT GGGGAACTAC TCTGCTGTGA TACCTGCCCC 
TCTTCCTACC ATATCCACTG CTTGAACCCC CCACTCCCAG AGATCCCCAA CGGCGAATGG 
CTTTGTCCTC GTTGCACATG CCCTTCACTG AAAGGTAAAG TTCAGAAGAT CCTGATTTGG 
AAATGGGGGC AGCCACCATC CCCTACACCA GTGCCCCGAC CCCCTGATGC TGACCCCAAT 
ACCCCTTCTC CTAAGCCTTT AGAGGGGCGA CCTGAGCGGC AATTCTTTGT GAAATGGCAA 
GGCATGTCCT ACTGGCACTG TTCTTGGGTG TCAGAACTAC AGCTGGAGCT TCACTGCCAA 
GTGATGTTCC GTAACTACCA GCGTAAGAAT GATATGGATG AGCCACCATC TGGTGACTTT 
GGTGGGGAGG AAGAAAAAAG CCGTAAACGA AAGAACAAGG ACCCCAAGTT TGCAGAAATG 
GAAGAACGTT TCTACCGCTA TGGGATTAAA CCTGAGTGGA TGATGATCCA CCGTATCCTC 
AACCACAGTG TAGATAAGAA GGGTCATGTC CACTATTTGA TCAAATGGAG GGACCTGCCC 
TATGATCAGG CATCCTGGGA GAGTGAAGAT GTAGAGGTGC AAGACTATGA CCTATTCAAG 
CAGAGCTATT GGAACCATAG GGAGCTAATG CGGGGTGAGG AAGGACGACC TGGCAAAAAG 
CTCAAGAAGG TTAAGCTTAG GAAGTTGGAG AGGCCTCCTG AAACTCCTAC AGTTGATCCA 
ACAGTGAAAT ATGAGCGGCA ACCAGAGTAC TTAGATGCCA CTGGTGGGAC TCTACATCCC 
TATCAAATGG AGGGATTGAA CTGGTTGCGC TTCTCCTGGG CCCAGGGCAC TGACACTATC 
TTGGCAGATG AAATGGGCTT GGGCAAGACG GTTCAGACCG CTGTCTTCCT CTATTCTTTG 
TACAAGGAGG GTCATTCAAA AGGCCCTTTC CTAGTGAGTG CCCCACTTTC TACAATCATC 
AACTGGGAGC GAGAGTTTGA GATGTGGGCT CCAGACATGT ATGTGGTAAC CTATGTTGGA 
GACAAGGATA GTCGTGCTAT TATTCGTGAA AACGAGTTCT CTTTTGAAGA CAATGCTATC 
CGTGGTGGCA AGAAGGCTTC CAGAATGAAG AAAGAAGCAT CTGTGAAGTT TCATGTTTTG 
CTGACTTCCT ATGAACTGAT TACCATTGAC ATGGCCATTC TGGGCTCCAT TGATTGGGCC 
TGCCTAATTG TGGATGAAGC TCATAGGCTT AAGAACAATC AGTCTAAGTT TTTCAGGGTA 
CTCAATGGCT ATTCCCTCCA GCACAAGTTA CTACTAACAG GGACTCCTCT ACAGAATAAC 
TTGGAGGAGC TTTTCCACCT GCTTAATTTC CTCACTCCAG AAAGATTCCA CAACCTGGAG 
GGCTTCCTAG AAGAATTTGC TGACATTGCC AAGGAAGACC AGATCAAAAA ATTGCATGAC 
ATGTTGGGGC CTCATATGCT TCGACGGCTT AAAGCTGATG TCTTCAAGAA CATGCCCTCT 
AAGACAGAGC TGATTGTGCG TGTGGAACTG AGCCCGATGC AGAAGAAATA CTACAAGTAC 
ATTCTAACCC GAAACTTTGA GGCACTCAAT GCTCGGGGTG GTGGTAATCA GGTTTCCCTA 
CTTAATGTGG TGATGGATCT AAAAAAGTGC TGTAACCACC CTTACCTCTT CCCTGTGGCT 
GCCATGGAAG CACCAAAGAT GCCCAATGGG ATGTATGATG GCAGTGCTCT AATTCGAGCA 
TCAGGGAAGC TACTTTTATT GCAAAAAATG CTCAAGAACC TCAAAGAGGG TGGTCACCGT 
GTGCTCATTT TCTCGCAGAT GACCAAGATG TTGGATCTGT TAGAGGATTT TTTGGAACAT 
GAAGGTTATA AATATGAGCG AATCGATGGG GGAATTACTG GGAACATGCG TCAGGAAGCC 
ATTGATCGTT TTAACGCACC TGGGGCTCAG CAGTTTTGCT TTCTTCTTTC TACTCGAGCT 
GGGGGCCTGG GAATCAATCT AGCTACTGCA GATACTGTTA TCATCTATGA CTCTGATTGG 
AACCCCCATA ATGACATCCA GGCTTTTAGC AGAGCCCATC GAATTGGTCA GAATAAGAAA 
GTGATGATCT ACCGTTTTGT GACCCGCGCT TCAGTGGAGG AGCGCATCAC ACAGGTGGCG 
AAGAAGAAGA TGATGCTTAC ACATCTTGTA GTAAGGCCTG GCCTGGGCTC TAAGACTGGC 
TCCATGTCCA AGCAGGAGCT TGATGATATC CTCAAGTTTG GCACTGAGGA ATTATTCAAA 
GATGAAGCCA CTGATGGAGG AGGAGACAAC AAGGAAGGAG AAGACAGTAG TGTTATTCAC 
TATGATGACA AGGCCATAGA GCGACTGCTG GATCGAAATC AGGATGAAAC AGAAGACACA 
GAACTTCAAG GCATGAATGA ATATTTGAGT TCCTTCAAAG TGGCCCAGTA TGTAGTACGG 
GAAGAAGAAA TGGGGGAGGA AGAAGAAGTA GAAAGAGAGA TCATCAAGCA GGAAGAAAGT 
GTGGATCCTG ACTACTGGGA GAAGCTGCTG CGACACCATT ATGAGCAGCA GCAAGAGGAT 
CTGGCCCGAA ACCTAGGCAA AGGGAAGCGA ATTCGTAAGC AGGTCAACTA CAACGATGGC 
TCCCAGGAGG ATCGAGATTG GCAGGATGAC CAGTCAGACA ATCAGTCCGA CTACTCAGTG 
GCCTCAGAGG AAGGCGATGA GGACTTTGAT GAGCGTTCAG AAGCTCCCAG ACGGCCTAGC 
CGCAAGGGCC TGCGAAATGA TAAGGACAAA CCATTGCCTC CATTGTTGGC TCGTGTTGGT 
GGAAATATTG AGGTACTTGG CTTCAATGCC CGCCAGCGGA AGGCTTTCCT CAATGCCATT 
ATGCGTTATG GGATGCCTCC TCAGGATGCC TTTACTACCC AATGGCTTGT GCGGGACCTG 
AGGGGCAAGT CAGAGAAAGA GTTCAAGGCA TATGTGTCTC TCTTTATGCG TCACCTTTGT 
GAACCAGGAG CAGATGGGGC TGAGACCTTT GCTGATGGTG TACCCCGTGA GGGCTTATCC 
CGGCAGCATG TTCTTACTCG TATTGGTGTT ATGTCACTTA TCCGAAAAAA GGTTCAGGAA 
TTTGAGCATG TCAATGGGCG CTGGAGCATG CCTGAGCTGG CTGAGGTAGA AGAGAACAAG 
AAGATGTCAC AGCCAGGATC ACCTTCACCT AAAACTCCCA CACCTTCCAC TCCAGGAGAC 
ACACAACCCA ACACTCCTGC ACCTGTGCCA CCCCCTGATG ATGGGGTGAA AGCAGAGGAG 
AATAATAACA AAGAAGAAGA GAACACAGAA GGAGAGAGAG AGGTGAAACC TGCAGCTCCT 
GAGGCTTCTG TGGAGGCTAC ACAGCCCTCT GCATCTAACC CAGAAGAGGA AAAAGTTCTG 
ACTGAGCCTC TGGAAGGAGA AGAGAAGGTG GATAAGCCAG AAGTCAAAGA GAAGACAGAG 
GAGACCATGG AGACAGAAAC CAAAGGTGTT GCTGAAACGG AAAAGGTAGA GGAGAAAGCA 
ACAGTGGATC TGACCCCTAT TGTGGTAGAA GACAAAGAGG AGAAGAAAGA AGAAGAAGAG 
AAAAAAGAAG TGATGCTTCA GAATGGGGAG ACTCCCAAGG ACCTGGGTGA TGAGAAGCAG 
AAGAAGAACA TCAAGCAGCG TTTTATGTTC AACATTGCAG ATGGTGGCTT CACTGAGCTG 
CATTCCCTTT GGCAGAATGA GGAACGGGCA GCCACAGTTA CAAAGAAGAC CTATGAGATC 
TGGCATCGGC GGCATGACTA CTGGCTGCTG GCTGGCATTA TTAACCATGG CTATGCTCGG 
TGGCAGGACA TTCAGAATGA CCCGCGCTAT GCCATTCTTA ATGAACCCTT CAAAGGTGAA 
ATGAATCGTG GTAACTTCTT GGAGATAAAA AACAAATTCC TGGCTAGAAG ATTTAAGCTC 
TTGGAACAAG CCTTGGTGAT TGAGGAGCAA CTGCGTCGAG CAGCTTACCT GAACATGTCA 
GAGGACCCTT CCCATCCTTC CATGGCCCTC AATACTCGTT TTGCTGAAGT GGAATGCCTG 
GCTGAGAGCC ACCAGCACCT GTCTAAGGAG TCAATGGCAG GAAATAAGCC AGCTAATGCT 
GTCCTGCACA AAGTTCTGAA ACAACTGGAG GAATTGTTGA GTGACATGAA GGCTGATGTC 
ACAAGGCTGC CAGCCACAAT TGCCCGTATT CCCCCTGTGG CTGTGCGGCT ACAGATGTCA 
GAGCGCAACA TCCTCAGCCG GCTGGCAAAC CGGGGCCCTG AACCCACCCC ACAGCAGGTA 
GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031794141.1
CDS	219..5933
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKEPKSSGQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKASPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRATKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRGPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_031938281.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031938281.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC TGGGAGACAG TTCTGGGGAG 
GGGCCTGAAT TTGTGGAGGA AGAAGATGAG ATGGTTTTGC GATCAGACAG CGAGGGCAGT 
GACTATACCC CTGGCAAGAA AAAGAAGAAG AAGCTTGGAC CTAAGAAGGA GAAGAAAAGC 
AAGTCTAAGA GAAAGGAGGA AGAAGAAGAA GATGATGATG ATGATGACTC AAAGGAGCCC 
AAGTCATCAG GTCAGCTACT AGAAGATTGG GGCATGGAGG ACATTGACCA TGTGTTCTCA 
GAAGAAGATT ATCGAACTCT TACCAACTAT AAGGCCTTCA GCCAGTTTGT CAGACCTCTC 
ATTGCTGCTA AGAACCCCAA GATTGCAGTT TCCAAGATGA TGATGGTTCT AGGAGCCAAA 
TGGCGAGAAT TCAGTACCAA CAACCCCTTC AAGGGCAGTT CCGGGGCCTC AGTGGCAGCT 
GCAGCTGCGG CAGCAGTAGC TGTAGTGGAG AGTATGGTAA CTGCAACTGA AGTTGCTCCA 
CCTCCACCCC CTGTGGAAGT ACCCATCAGA AAGGCCAAAA CCAAGGAGGG TAAAGGTCCC 
AATGCCCGAC GGAAACCTAA AGCTAGCCCC CGTGTGCCTG ATGCTAAGAA GCCCAAACCG 
AAGAAGGTGG CACCCCTGAA AATCAAACTG GGAGGCTTTG GCTCTAAGCG CAAGAGGTCT 
TCGAGTGAAG ATGATGACCT GGATGTGGAA TCTGACTTTG ATGATGCCAG TATCAACAGT 
TACTCCGTGT CAGATGGTTC GACTAGTAGG AGCAGCCGCA GCCGCAAGAA ACTTAGAGCT 
ACCAAAAAGA AAAAGAAAGG CGAGGAGGAG GTGACTGCTG TGGATGGTTA TGAGACAGAC 
CACCAGGACT ACTGTGAAGT GTGCCAGCAA GGAGGAGAGA TCATCCTGTG TGATACCTGT 
CCCCGTGCTT ACCACATGGT CTGCCTGGAT CCTGACATGG AGAAGGCCCC GGAGGGCAAA 
TGGAGTTGTC CACACTGTGA AAAGGAAGGT ATCCAGTGGG AAGCAAAGGA GGACAACTCT 
GAGGGTGAAG AAATCCTAGA AGAAGTTGGT GGTGACCCTG AGGAGGAGGA TGATCATCAC 
ATGGAGTTTT GCCGTGTCTG CAAGGATGGT GGGGAACTAC TCTGCTGTGA TACCTGCCCC 
TCTTCCTACC ATATCCACTG CTTGAACCCC CCACTCCCAG AGATCCCCAA CGGCGAATGG 
CTTTGTCCTC GTTGCACATG CCCTTCACTG AAAGGTAAAG TTCAGAAGAT CCTGATTTGG 
AAATGGGGGC AGCCACCATC CCCTACACCA GTGCCCCGAC CCCCTGATGC TGACCCCAAT 
ACCCCTTCTC CTAAGCCTTT AGAGGGGCGA CCTGAGCGGC AATTCTTTGT GAAATGGCAA 
GGCATGTCCT ACTGGCACTG TTCTTGGGTG TCAGAACTAC AGCTGGAGCT TCACTGCCAA 
GTGATGTTCC GTAACTACCA GCGTAAGAAT GATATGGATG AGCCACCATC TGGTGACTTT 
GGTGGGGAGG AAGAAAAAAG CCGTAAACGA AAGAACAAGG ACCCCAAGTT TGCAGAAATG 
GAAGAACGTT TCTACCGCTA TGGGATTAAA CCTGAGTGGA TGATGATCCA CCGTATCCTC 
AACCACAGTG TAGATAAGAA GGGTCATGTC CACTATTTGA TCAAATGGAG GGACCTGCCC 
TATGATCAGG CATCCTGGGA GAGTGAAGAT GTAGAGGTGC AAGACTATGA CCTATTCAAG 
CAGAGCTATT GGAACCATAG GGAGCTAATG CGGGGTGAGG AAGGACGACC TGGCAAAAAG 
CTCAAGAAGG TTAAGCTTAG GAAGTTGGAG AGGCCTCCTG AAACTCCTAC AGTTGATCCA 
ACAGTGAAAT ATGAGCGGCA ACCAGAGTAC TTAGATGCCA CTGGTGGGAC TCTACATCCC 
TATCAAATGG AGGGATTGAA CTGGTTGCGC TTCTCCTGGG CCCAGGGCAC TGACACTATC 
TTGGCAGATG AAATGGGCTT GGGCAAGACG GTTCAGACCG CTGTCTTCCT CTATTCTTTG 
TACAAGGAGG GTCATTCAAA AGGCCCTTTC CTAGTGAGTG CCCCACTTTC TACAATCATC 
AACTGGGAGC GAGAGTTTGA GATGTGGGCT CCAGACATGT ATGTGGTAAC CTATGTTGGA 
GACAAGGATA GTCGTGCTAT TATTCGTGAA AACGAGTTCT CTTTTGAAGA CAATGCTATC 
CGTGGTGGCA AGAAGGCTTC CAGAATGAAG AAAGAAGCAT CTGTGAAGTT TCATGTTTTG 
CTGACTTCCT ATGAACTGAT TACCATTGAC ATGGCCATTC TGGGCTCCAT TGATTGGGCC 
TGCCTAATTG TGGATGAAGC TCATAGGCTT AAGAACAATC AGTCTAAGTT TTTCAGGGTA 
CTCAATGGCT ATTCCCTCCA GCACAAGTTA CTACTAACAG GGACTCCTCT ACAGAATAAC 
TTGGAGGAGC TTTTCCACCT GCTTAATTTC CTCACTCCAG AAAGATTCCA CAACCTGGAG 
GGCTTCCTAG AAGAATTTGC TGACATTGCC AAGGAAGACC AGATCAAAAA ATTGCATGAC 
ATGTTGGGGC CTCATATGCT TCGACGGCTT AAAGCTGATG TCTTCAAGAA CATGCCCTCT 
AAGACAGAGC TGATTGTGCG TGTGGAACTG AGCCCGATGC AGAAGAAATA CTACAAGTAC 
ATTCTAACCC GAAACTTTGA GGCACTCAAT GCTCGGGGTG GTGGTAATCA GGTTTCCCTA 
CTTAATGTGG TGATGGATCT AAAAAAGTGC TGTAACCACC CTTACCTCTT CCCTGTGGCT 
GCCATGGAAG CACCAAAGAT GCCCAATGGG ATGTATGATG GCAGTGCTCT AATTCGAGCA 
TCAGGGAAGC TACTTTTATT GCAAAAAATG CTCAAGAACC TCAAAGAGGG TGGTCACCGT 
GTGCTCATTT TCTCGCAGAT GACCAAGATG TTGGATCTGT TAGAGGATTT TTTGGAACAT 
GAAGGTTATA AATATGAGCG AATCGATGGG GGAATTACTG GGAACATGCG TCAGGAAGCC 
ATTGATCGTT TTAACGCACC TGGGGCTCAG CAGTTTTGCT TTCTTCTTTC TACTCGAGCT 
GGGGGCCTGG GAATCAATCT AGCTACTGCA GATACTGTTA TCATCTATGA CTCTGATTGG 
AACCCCCATA ATGACATCCA GGCTTTTAGC AGAGCCCATC GAATTGGTCA GAATAAGAAA 
GTGATGATCT ACCGTTTTGT GACCCGCGCT TCAGTGGAGG AGCGCATCAC ACAGGTGGCG 
AAGAAGAAGA TGATGCTTAC ACATCTTGTA GTAAGGCCTG GCCTGGGCTC TAAGACTGGC 
TCCATGTCCA AGCAGGAGCT TGATGATATC CTCAAGTTTG GCACTGAGGA ATTATTCAAA 
GATGAAGCCA CTGATGGAGG AGGAGACAAC AAGGAAGGAG AAGACAGTAG TGTTATTCAC 
TATGATGACA AGGCCATAGA GCGACTGCTG GATCGAAATC AGGATGAAAC AGAAGACACA 
GAACTTCAAG GCATGAATGA ATATTTGAGT TCCTTCAAAG TGGCCCAGTA TGTAGTACGG 
GAAGAAGAAA TGGGGGAGGA AGAAGAAGTA GAAAGAGAGA TCATCAAGCA GGAAGAAAGT 
GTGGATCCTG ACTACTGGGA GAAGCTGCTG CGACACCATT ATGAGCAGCA GCAAGAGGAT 
CTGGCCCGAA ACCTAGGCAA AGGGAAGCGA ATTCGTAAGC AGGTCAACTA CAACGATGGC 
TCCCAGGAGG ATCGAGATTG GCAGGATGAC CAGTCAGACA ATCAGTCCGA CTACTCAGTG 
GCCTCAGAGG AAGGCGATGA GGACTTTGAT GAGCGTTCAG AAGCTCCCAG ACGGCCTAGC 
CGCAAGGGCC TGCGAAATGA TAAGGACAAA CCATTGCCTC CATTGTTGGC TCGTGTTGGT 
GGAAATATTG AGGTACTTGG CTTCAATGCC CGCCAGCGGA AGGCTTTCCT CAATGCCATT 
ATGCGTTATG GGATGCCTCC TCAGGATGCC TTTACTACCC AATGGCTTGT GCGGGACCTG 
AGGGGCAAGT CAGAGAAAGA GTTCAAGGCA TATGTGTCTC TCTTTATGCG TCACCTTTGT 
GAACCAGGAG CAGATGGGGC TGAGACCTTT GCTGATGGTG TACCCCGTGA GGGCTTATCC 
CGGCAGCATG TTCTTACTCG TATTGGTGTT ATGTCACTTA TCCGAAAAAA GGTTCAGGAA 
TTTGAGCATG TCAATGGGCG CTGGAGCATG CCTGAGCTGG CTGAGGTAGA AGAGAACAAG 
AAGATGTCAC AGCCAGGATC ACCTTCACCT AAAACTCCCA CACCTTCCAC TCCAGGAGAC 
ACACAACCCA ACACTCCTGC ACCTGTGCCA CCCCCTGATG ATGGGGTGAA AGCAGAGGAG 
AATAATAACA AAGAAGAAGA GAACACAGAA GGAGAGAGAG AGGTGAAACC TGCAGCTCCT 
GAGGCTTCTG TGGAGGCTAC ACAGCCCTCT GCATCTAACC CAGAAGAGGA AAAAGTTCTG 
ACTGAGCCTC TGGAAGGAGA AGAGAAGGTG GATAAGCCAG AAGTCAAAGA GAAGACAGAG 
GAGACCATGG AGACAGAAAC CAAAGGTGTT GCTGAAACGG AAAAGGTAGA GGAGAAAGCA 
ACAGTGGATC TGACCCCTAT TGTGGTAGAA GACAAAGAGG AGAAGAAAGA AGAAGAAGAG 
AAAAAAGAAG TGATGCTTCA GAATGGGGAG ACTCCCAAGG ACCTGGGTGA TGAGAAGCAG 
AAGAAGAACA TCAAGCAGCG TTTTATGTTC AACATTGCAG ATGGTGGCTT CACTGAGCTG 
CATTCCCTTT GGCAGAATGA GGAACGGGCA GCCACAGTTA CAAAGAAGAC CTATGAGATC 
TGGCATCGGC GGCATGACTA CTGGCTGCTG GCTGGCATTA TTAACCATGG CTATGCTCGG 
TGGCAGGACA TTCAGAATGA CCCGCGCTAT GCCATTCTTA ATGAACCCTT CAAAGGTGAA 
ATGAATCGTG GTAACTTCTT GGAGATAAAA AACAAATTCC TGGCTAGAAG ATTTAAGCTC 
TTGGAACAAG CCTTGGTGAT TGAGGAGCAA CTGCGTCGAG CAGCTTACCT GAACATGTCA 
GAGGACCCTT CCCATCCTTC CATGGCCCTC AATACTCGTT TTGCTGAAGT GGAATGCCTG 
GCTGAGAGCC ACCAGCACCT GTCTAAGGAG TCAATGGCAG GAAATAAGCC AGCTAATGCT 
GTCCTGCACA AAGTTCTGAA ACAACTGGAG GAATTGTTGA GTGACATGAA GGCTGATGTC 
ACAAGGCTGC CAGCCACAAT TGCCCGTATT CCCCCTGTGG CTGTGCGGCT ACAGATGTCA 
GAGCGCAACA TCCTCAGCCG GCTGGCAAAC CGGGGCCCTG AACCCACCCC ACAGCAGGTA 
GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_031938282.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031794142.1 Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.

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CloneID	OSa236826
Clone ID Related Accession (Same CDS sequence)	XM_031938282.1
Accession Version	XM_031938282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG ACCACCAGGA CTACTGTGAA 
GTGTGCCAGC AAGGAGGAGA GATCATCCTG TGTGATACCT GTCCCCGTGC TTACCACATG 
GTCTGCCTGG ATCCTGACAT GGAGAAGGCC CCGGAGGGCA AATGGAGTTG TCCACACTGT 
GAAAAGGAAG GTATCCAGTG GGAAGCAAAG GAGGACAACT CTGAGGGTGA AGAAATCCTA 
GAAGAAGTTG GTGGTGACCC TGAGGAGGAG GATGATCATC ACATGGAGTT TTGCCGTGTC 
TGCAAGGATG GTGGGGAACT ACTCTGCTGT GATACCTGCC CCTCTTCCTA CCATATCCAC 
TGCTTGAACC CCCCACTCCC AGAGATCCCC AACGGCGAAT GGCTTTGTCC TCGTTGCACA 
TGCCCTTCAC TGAAAGGTAA AGTTCAGAAG ATCCTGATTT GGAAATGGGG GCAGCCACCA 
TCCCCTACAC CAGTGCCCCG ACCCCCTGAT GCTGACCCCA ATACCCCTTC TCCTAAGCCT 
TTAGAGGGGC GACCTGAGCG GCAATTCTTT GTGAAATGGC AAGGCATGTC CTACTGGCAC 
TGTTCTTGGG TGTCAGAACT ACAGCTGGAG CTTCACTGCC AAGTGATGTT CCGTAACTAC 
CAGCGTAAGA ATGATATGGA TGAGCCACCA TCTGGTGACT TTGGTGGGGA GGAAGAAAAA 
AGCCGTAAAC GAAAGAACAA GGACCCCAAG TTTGCAGAAA TGGAAGAACG TTTCTACCGC 
TATGGGATTA AACCTGAGTG GATGATGATC CACCGTATCC TCAACCACAG TGTAGATAAG 
AAGGGTCATG TCCACTATTT GATCAAATGG AGGGACCTGC CCTATGATCA GGCATCCTGG 
GAGAGTGAAG ATGTAGAGGT GCAAGACTAT GACCTATTCA AGCAGAGCTA TTGGAACCAT 
AGGGAGCTAA TGCGGGGTGA GGAAGGACGA CCTGGCAAAA AGCTCAAGAA GGTTAAGCTT 
AGGAAGTTGG AGAGGCCTCC TGAAACTCCT ACAGTTGATC CAACAGTGAA ATATGAGCGG 
CAACCAGAGT ACTTAGATGC CACTGGTGGG ACTCTACATC CCTATCAAAT GGAGGGATTG 
AACTGGTTGC GCTTCTCCTG GGCCCAGGGC ACTGACACTA TCTTGGCAGA TGAAATGGGC 
TTGGGCAAGA CGGTTCAGAC CGCTGTCTTC CTCTATTCTT TGTACAAGGA GGGTCATTCA 
AAAGGCCCTT TCCTAGTGAG TGCCCCACTT TCTACAATCA TCAACTGGGA GCGAGAGTTT 
GAGATGTGGG CTCCAGACAT GTATGTGGTA ACCTATGTTG GAGACAAGGA TAGTCGTGCT 
ATTATTCGTG AAAACGAGTT CTCTTTTGAA GACAATGCTA TCCGTGGTGG CAAGAAGGCT 
TCCAGAATGA AGAAAGAAGC ATCTGTGAAG TTTCATGTTT TGCTGACTTC CTATGAACTG 
ATTACCATTG ACATGGCCAT TCTGGGCTCC ATTGATTGGG CCTGCCTAAT TGTGGATGAA 
GCTCATAGGC TTAAGAACAA TCAGTCTAAG TTTTTCAGGG TACTCAATGG CTATTCCCTC 
CAGCACAAGT TACTACTAAC AGGGACTCCT CTACAGAATA ACTTGGAGGA GCTTTTCCAC 
CTGCTTAATT TCCTCACTCC AGAAAGATTC CACAACCTGG AGGGCTTCCT AGAAGAATTT 
GCTGACATTG CCAAGGAAGA CCAGATCAAA AAATTGCATG ACATGTTGGG GCCTCATATG 
CTTCGACGGC TTAAAGCTGA TGTCTTCAAG AACATGCCCT CTAAGACAGA GCTGATTGTG 
CGTGTGGAAC TGAGCCCGAT GCAGAAGAAA TACTACAAGT ACATTCTAAC CCGAAACTTT 
GAGGCACTCA ATGCTCGGGG TGGTGGTAAT CAGGTTTCCC TACTTAATGT GGTGATGGAT 
CTAAAAAAGT GCTGTAACCA CCCTTACCTC TTCCCTGTGG CTGCCATGGA AGCACCAAAG 
ATGCCCAATG GGATGTATGA TGGCAGTGCT CTAATTCGAG CATCAGGGAA GCTACTTTTA 
TTGCAAAAAA TGCTCAAGAA CCTCAAAGAG GGTGGTCACC GTGTGCTCAT TTTCTCGCAG 
ATGACCAAGA TGTTGGATCT GTTAGAGGAT TTTTTGGAAC ATGAAGGTTA TAAATATGAG 
CGAATCGATG GGGGAATTAC TGGGAACATG CGTCAGGAAG CCATTGATCG TTTTAACGCA 
CCTGGGGCTC AGCAGTTTTG CTTTCTTCTT TCTACTCGAG CTGGGGGCCT GGGAATCAAT 
CTAGCTACTG CAGATACTGT TATCATCTAT GACTCTGATT GGAACCCCCA TAATGACATC 
CAGGCTTTTA GCAGAGCCCA TCGAATTGGT CAGAATAAGA AAGTGATGAT CTACCGTTTT 
GTGACCCGCG CTTCAGTGGA GGAGCGCATC ACACAGGTGG CGAAGAAGAA GATGATGCTT 
ACACATCTTG TAGTAAGGCC TGGCCTGGGC TCTAAGACTG GCTCCATGTC CAAGCAGGAG 
CTTGATGATA TCCTCAAGTT TGGCACTGAG GAATTATTCA AAGATGAAGC CACTGATGGA 
GGAGGAGACA ACAAGGAAGG AGAAGACAGT AGTGTTATTC ACTATGATGA CAAGGCCATA 
GAGCGACTGC TGGATCGAAA TCAGGATGAA ACAGAAGACA CAGAACTTCA AGGCATGAAT 
GAATATTTGA GTTCCTTCAA AGTGGCCCAG TATGTAGTAC GGGAAGAAGA AATGGGGGAG 
GAAGAAGAAG TAGAAAGAGA GATCATCAAG CAGGAAGAAA GTGTGGATCC TGACTACTGG 
GAGAAGCTGC TGCGACACCA TTATGAGCAG CAGCAAGAGG ATCTGGCCCG AAACCTAGGC 
AAAGGGAAGC GAATTCGTAA GCAGGTCAAC TACAACGATG GCTCCCAGGA GGATCGAGAT 
TGGCAGGATG ACCAGTCAGA CAATCAGTCC GACTACTCAG TGGCCTCAGA GGAAGGCGAT 
GAGGACTTTG ATGAGCGTTC AGAAGCTCCC AGACGGCCTA GCCGCAAGGG CCTGCGAAAT 
GATAAGGACA AACCATTGCC TCCATTGTTG GCTCGTGTTG GTGGAAATAT TGAGGTACTT 
GGCTTCAATG CCCGCCAGCG GAAGGCTTTC CTCAATGCCA TTATGCGTTA TGGGATGCCT 
CCTCAGGATG CCTTTACTAC CCAATGGCTT GTGCGGGACC TGAGGGGCAA GTCAGAGAAA 
GAGTTCAAGG CATATGTGTC TCTCTTTATG CGTCACCTTT GTGAACCAGG AGCAGATGGG 
GCTGAGACCT TTGCTGATGG TGTACCCCGT GAGGGCTTAT CCCGGCAGCA TGTTCTTACT 
CGTATTGGTG TTATGTCACT TATCCGAAAA AAGGTTCAGG AATTTGAGCA TGTCAATGGG 
CGCTGGAGCA TGCCTGAGCT GGCTGAGGTA GAAGAGAACA AGAAGATGTC ACAGCCAGGA 
TCACCTTCAC CTAAAACTCC CACACCTTCC ACTCCAGGAG ACACACAACC CAACACTCCT 
GCACCTGTGC CACCCCCTGA TGATGGGGTG AAAGCAGAGG AGAATAATAA CAAAGAAGAA 
GAGAACACAG AAGGAGAGAG AGAGGTGAAA CCTGCAGCTC CTGAGGCTTC TGTGGAGGCT 
ACACAGCCCT CTGCATCTAA CCCAGAAGAG GAAAAAGTTC TGACTGAGCC TCTGGAAGGA 
GAAGAGAAGG TGGATAAGCC AGAAGTCAAA GAGAAGACAG AGGAGACCAT GGAGACAGAA 
ACCAAAGGTG TTGCTGAAAC GGAAAAGGTA GAGGAGAAAG CAACAGTGGA TCTGACCCCT 
ATTGTGGTAG AAGACAAAGA GGAGAAGAAA GAAGAAGAAG AGAAAAAAGA AGTGATGCTT 
CAGAATGGGG AGACTCCCAA GGACCTGGGT GATGAGAAGC AGAAGAAGAA CATCAAGCAG 
CGTTTTATGT TCAACATTGC AGATGGTGGC TTCACTGAGC TGCATTCCCT TTGGCAGAAT 
GAGGAACGGG CAGCCACAGT TACAAAGAAG ACCTATGAGA TCTGGCATCG GCGGCATGAC 
TACTGGCTGC TGGCTGGCAT TATTAACCAT GGCTATGCTC GGTGGCAGGA CATTCAGAAT 
GACCCGCGCT ATGCCATTCT TAATGAACCC TTCAAAGGTG AAATGAATCG TGGTAACTTC 
TTGGAGATAA AAAACAAATT CCTGGCTAGA AGATTTAAGC TCTTGGAACA AGCCTTGGTG 
ATTGAGGAGC AACTGCGTCG AGCAGCTTAC CTGAACATGT CAGAGGACCC TTCCCATCCT 
TCCATGGCCC TCAATACTCG TTTTGCTGAA GTGGAATGCC TGGCTGAGAG CCACCAGCAC 
CTGTCTAAGG AGTCAATGGC AGGAAATAAG CCAGCTAATG CTGTCCTGCA CAAAGGTATT 
CTGAAACAAC TGGAGGAATT GTTGAGTGAC ATGAAGGCTG ATGTCACAAG GCTGCCAGCC 
ACAATTGCCC GTATTCCCCC TGTGGCTGTG CGGCTACAGA TGTCAGAGCG CAACATCCTC 
AGCCGGCTGG CAAACCGGGG CCCTGAACCC ACCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031794142.1
CDS	220..5919
Translation	MASGLGSPSPCSAGSEEEDMEVLLNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERVLLCRQLGDSSGEGPEFVEEEDEMVLRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKEPKSSGQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKASPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRATKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPSLKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGEEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEVQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPPDDGVKAEENNNKEEENTEGEREVKPAAPEASVEATQPSASNPEEEKVLTEPLEGEEKVDKPEVKEKTEETMETETKGVAETEKVEEKATVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLGDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRGPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_031938282.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031938282.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCATCCCCC TGCTCAGCAG GCAGTGAGGA AGAGGACATG 
GAGGTGCTTC TAAACAGCCT TCCCCCACCC CATCCAGAGA ATGAAGAAGA CCCAGAGGAA 
GACTTATCAG AGGCAGAGAC TCCTAAACTC AAAAAGAAGA AGAAGCCTAA GAAACCTCGG 
GACCCTAAAA TCCCAAAGAG CAAACGACAA AAAAAGGAGC GTGTGCTCTT ATGCCGGCAG 
CTGGGAGACA GTTCTGGGGA GGGGCCTGAA TTTGTGGAGG AAGAAGATGA GATGGTTTTG 
CGATCAGACA GCGAGGGCAG TGACTATACC CCTGGCAAGA AAAAGAAGAA GAAGCTTGGA 
CCTAAGAAGG AGAAGAAAAG CAAGTCTAAG AGAAAGGAGG AAGAAGAAGA AGATGATGAT 
GATGATGACT CAAAGGAGCC CAAGTCATCA GGTCAGCTAC TAGAAGATTG GGGCATGGAG 
GACATTGACC ATGTGTTCTC AGAAGAAGAT TATCGAACTC TTACCAACTA TAAGGCCTTC 
AGCCAGTTTG TCAGACCTCT CATTGCTGCT AAGAACCCCA AGATTGCAGT TTCCAAGATG 
ATGATGGTTC TAGGAGCCAA ATGGCGAGAA TTCAGTACCA ACAACCCCTT CAAGGGCAGT 
TCCGGGGCCT CAGTGGCAGC TGCAGCTGCG GCAGCAGTAG CTGTAGTGGA GAGTATGGTA 
ACTGCAACTG AAGTTGCTCC ACCTCCACCC CCTGTGGAAG TACCCATCAG AAAGGCCAAA 
ACCAAGGAGG GTAAAGGTCC CAATGCCCGA CGGAAACCTA AAGCTAGCCC CCGTGTGCCT 
GATGCTAAGA AGCCCAAACC GAAGAAGGTG GCACCCCTGA AAATCAAACT GGGAGGCTTT 
GGCTCTAAGC GCAAGAGGTC TTCGAGTGAA GATGATGACC TGGATGTGGA ATCTGACTTT 
GATGATGCCA GTATCAACAG TTACTCCGTG TCAGATGGTT CGACTAGTAG GAGCAGCCGC 
AGCCGCAAGA AACTTAGAGC TACCAAAAAG AAAAAGAAAG ACCACCAGGA CTACTGTGAA 
GTGTGCCAGC AAGGAGGAGA GATCATCCTG TGTGATACCT GTCCCCGTGC TTACCACATG 
GTCTGCCTGG ATCCTGACAT GGAGAAGGCC CCGGAGGGCA AATGGAGTTG TCCACACTGT 
GAAAAGGAAG GTATCCAGTG GGAAGCAAAG GAGGACAACT CTGAGGGTGA AGAAATCCTA 
GAAGAAGTTG GTGGTGACCC TGAGGAGGAG GATGATCATC ACATGGAGTT TTGCCGTGTC 
TGCAAGGATG GTGGGGAACT ACTCTGCTGT GATACCTGCC CCTCTTCCTA CCATATCCAC 
TGCTTGAACC CCCCACTCCC AGAGATCCCC AACGGCGAAT GGCTTTGTCC TCGTTGCACA 
TGCCCTTCAC TGAAAGGTAA AGTTCAGAAG ATCCTGATTT GGAAATGGGG GCAGCCACCA 
TCCCCTACAC CAGTGCCCCG ACCCCCTGAT GCTGACCCCA ATACCCCTTC TCCTAAGCCT 
TTAGAGGGGC GACCTGAGCG GCAATTCTTT GTGAAATGGC AAGGCATGTC CTACTGGCAC 
TGTTCTTGGG TGTCAGAACT ACAGCTGGAG CTTCACTGCC AAGTGATGTT CCGTAACTAC 
CAGCGTAAGA ATGATATGGA TGAGCCACCA TCTGGTGACT TTGGTGGGGA GGAAGAAAAA 
AGCCGTAAAC GAAAGAACAA GGACCCCAAG TTTGCAGAAA TGGAAGAACG TTTCTACCGC 
TATGGGATTA AACCTGAGTG GATGATGATC CACCGTATCC TCAACCACAG TGTAGATAAG 
AAGGGTCATG TCCACTATTT GATCAAATGG AGGGACCTGC CCTATGATCA GGCATCCTGG 
GAGAGTGAAG ATGTAGAGGT GCAAGACTAT GACCTATTCA AGCAGAGCTA TTGGAACCAT 
AGGGAGCTAA TGCGGGGTGA GGAAGGACGA CCTGGCAAAA AGCTCAAGAA GGTTAAGCTT 
AGGAAGTTGG AGAGGCCTCC TGAAACTCCT ACAGTTGATC CAACAGTGAA ATATGAGCGG 
CAACCAGAGT ACTTAGATGC CACTGGTGGG ACTCTACATC CCTATCAAAT GGAGGGATTG 
AACTGGTTGC GCTTCTCCTG GGCCCAGGGC ACTGACACTA TCTTGGCAGA TGAAATGGGC 
TTGGGCAAGA CGGTTCAGAC CGCTGTCTTC CTCTATTCTT TGTACAAGGA GGGTCATTCA 
AAAGGCCCTT TCCTAGTGAG TGCCCCACTT TCTACAATCA TCAACTGGGA GCGAGAGTTT 
GAGATGTGGG CTCCAGACAT GTATGTGGTA ACCTATGTTG GAGACAAGGA TAGTCGTGCT 
ATTATTCGTG AAAACGAGTT CTCTTTTGAA GACAATGCTA TCCGTGGTGG CAAGAAGGCT 
TCCAGAATGA AGAAAGAAGC ATCTGTGAAG TTTCATGTTT TGCTGACTTC CTATGAACTG 
ATTACCATTG ACATGGCCAT TCTGGGCTCC ATTGATTGGG CCTGCCTAAT TGTGGATGAA 
GCTCATAGGC TTAAGAACAA TCAGTCTAAG TTTTTCAGGG TACTCAATGG CTATTCCCTC 
CAGCACAAGT TACTACTAAC AGGGACTCCT CTACAGAATA ACTTGGAGGA GCTTTTCCAC 
CTGCTTAATT TCCTCACTCC AGAAAGATTC CACAACCTGG AGGGCTTCCT AGAAGAATTT 
GCTGACATTG CCAAGGAAGA CCAGATCAAA AAATTGCATG ACATGTTGGG GCCTCATATG 
CTTCGACGGC TTAAAGCTGA TGTCTTCAAG AACATGCCCT CTAAGACAGA GCTGATTGTG 
CGTGTGGAAC TGAGCCCGAT GCAGAAGAAA TACTACAAGT ACATTCTAAC CCGAAACTTT 
GAGGCACTCA ATGCTCGGGG TGGTGGTAAT CAGGTTTCCC TACTTAATGT GGTGATGGAT 
CTAAAAAAGT GCTGTAACCA CCCTTACCTC TTCCCTGTGG CTGCCATGGA AGCACCAAAG 
ATGCCCAATG GGATGTATGA TGGCAGTGCT CTAATTCGAG CATCAGGGAA GCTACTTTTA 
TTGCAAAAAA TGCTCAAGAA CCTCAAAGAG GGTGGTCACC GTGTGCTCAT TTTCTCGCAG 
ATGACCAAGA TGTTGGATCT GTTAGAGGAT TTTTTGGAAC ATGAAGGTTA TAAATATGAG 
CGAATCGATG GGGGAATTAC TGGGAACATG CGTCAGGAAG CCATTGATCG TTTTAACGCA 
CCTGGGGCTC AGCAGTTTTG CTTTCTTCTT TCTACTCGAG CTGGGGGCCT GGGAATCAAT 
CTAGCTACTG CAGATACTGT TATCATCTAT GACTCTGATT GGAACCCCCA TAATGACATC 
CAGGCTTTTA GCAGAGCCCA TCGAATTGGT CAGAATAAGA AAGTGATGAT CTACCGTTTT 
GTGACCCGCG CTTCAGTGGA GGAGCGCATC ACACAGGTGG CGAAGAAGAA GATGATGCTT 
ACACATCTTG TAGTAAGGCC TGGCCTGGGC TCTAAGACTG GCTCCATGTC CAAGCAGGAG 
CTTGATGATA TCCTCAAGTT TGGCACTGAG GAATTATTCA AAGATGAAGC CACTGATGGA 
GGAGGAGACA ACAAGGAAGG AGAAGACAGT AGTGTTATTC ACTATGATGA CAAGGCCATA 
GAGCGACTGC TGGATCGAAA TCAGGATGAA ACAGAAGACA CAGAACTTCA AGGCATGAAT 
GAATATTTGA GTTCCTTCAA AGTGGCCCAG TATGTAGTAC GGGAAGAAGA AATGGGGGAG 
GAAGAAGAAG TAGAAAGAGA GATCATCAAG CAGGAAGAAA GTGTGGATCC TGACTACTGG 
GAGAAGCTGC TGCGACACCA TTATGAGCAG CAGCAAGAGG ATCTGGCCCG AAACCTAGGC 
AAAGGGAAGC GAATTCGTAA GCAGGTCAAC TACAACGATG GCTCCCAGGA GGATCGAGAT 
TGGCAGGATG ACCAGTCAGA CAATCAGTCC GACTACTCAG TGGCCTCAGA GGAAGGCGAT 
GAGGACTTTG ATGAGCGTTC AGAAGCTCCC AGACGGCCTA GCCGCAAGGG CCTGCGAAAT 
GATAAGGACA AACCATTGCC TCCATTGTTG GCTCGTGTTG GTGGAAATAT TGAGGTACTT 
GGCTTCAATG CCCGCCAGCG GAAGGCTTTC CTCAATGCCA TTATGCGTTA TGGGATGCCT 
CCTCAGGATG CCTTTACTAC CCAATGGCTT GTGCGGGACC TGAGGGGCAA GTCAGAGAAA 
GAGTTCAAGG CATATGTGTC TCTCTTTATG CGTCACCTTT GTGAACCAGG AGCAGATGGG 
GCTGAGACCT TTGCTGATGG TGTACCCCGT GAGGGCTTAT CCCGGCAGCA TGTTCTTACT 
CGTATTGGTG TTATGTCACT TATCCGAAAA AAGGTTCAGG AATTTGAGCA TGTCAATGGG 
CGCTGGAGCA TGCCTGAGCT GGCTGAGGTA GAAGAGAACA AGAAGATGTC ACAGCCAGGA 
TCACCTTCAC CTAAAACTCC CACACCTTCC ACTCCAGGAG ACACACAACC CAACACTCCT 
GCACCTGTGC CACCCCCTGA TGATGGGGTG AAAGCAGAGG AGAATAATAA CAAAGAAGAA 
GAGAACACAG AAGGAGAGAG AGAGGTGAAA CCTGCAGCTC CTGAGGCTTC TGTGGAGGCT 
ACACAGCCCT CTGCATCTAA CCCAGAAGAG GAAAAAGTTC TGACTGAGCC TCTGGAAGGA 
GAAGAGAAGG TGGATAAGCC AGAAGTCAAA GAGAAGACAG AGGAGACCAT GGAGACAGAA 
ACCAAAGGTG TTGCTGAAAC GGAAAAGGTA GAGGAGAAAG CAACAGTGGA TCTGACCCCT 
ATTGTGGTAG AAGACAAAGA GGAGAAGAAA GAAGAAGAAG AGAAAAAAGA AGTGATGCTT 
CAGAATGGGG AGACTCCCAA GGACCTGGGT GATGAGAAGC AGAAGAAGAA CATCAAGCAG 
CGTTTTATGT TCAACATTGC AGATGGTGGC TTCACTGAGC TGCATTCCCT TTGGCAGAAT 
GAGGAACGGG CAGCCACAGT TACAAAGAAG ACCTATGAGA TCTGGCATCG GCGGCATGAC 
TACTGGCTGC TGGCTGGCAT TATTAACCAT GGCTATGCTC GGTGGCAGGA CATTCAGAAT 
GACCCGCGCT ATGCCATTCT TAATGAACCC TTCAAAGGTG AAATGAATCG TGGTAACTTC 
TTGGAGATAA AAAACAAATT CCTGGCTAGA AGATTTAAGC TCTTGGAACA AGCCTTGGTG 
ATTGAGGAGC AACTGCGTCG AGCAGCTTAC CTGAACATGT CAGAGGACCC TTCCCATCCT 
TCCATGGCCC TCAATACTCG TTTTGCTGAA GTGGAATGCC TGGCTGAGAG CCACCAGCAC 
CTGTCTAAGG AGTCAATGGC AGGAAATAAG CCAGCTAATG CTGTCCTGCA CAAAGGTATT 
CTGAAACAAC TGGAGGAATT GTTGAGTGAC ATGAAGGCTG ATGTCACAAG GCTGCCAGCC 
ACAATTGCCC GTATTCCCCC TGTGGCTGTG CGGCTACAGA TGTCAGAGCG CAACATCCTC 
AGCCGGCTGG CAAACCGGGG CCCTGAACCC ACCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CHD4 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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