ORF » Species Summary » Sarcophilus harrisii » LOC100920521 cDNA ORF clone

LOC100920521 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

The following LOC100920521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100920521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSa121356 XM_012541284.3
Latest version!		 Sarcophilus harrisii cytochrome P450 7B1 (LOC100920521), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OSa121356 XM_012541284.1 Sarcophilus harrisii 25-hydroxycholesterol 7-alpha-hydroxylase (LOC100920521), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OSa121356 XM_012541284.2 Sarcophilus harrisii 25-hydroxycholesterol 7-alpha-hydroxylase (LOC100920521), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC100920521 ( XM_012541284.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012396738.1 Sarcophilus harrisii cytochrome P450 7B1 (LOC100920521), mRNA.
    Price & Availability↑
    CloneID OSa121356
    Clone ID Related Accession (Same CDS sequence) XM_012541284.3 , XM_012541284.1 , XM_012541284.2
    Accession Version XM_012541284.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1577635200000
    Organism Sarcophilus harrisii(Tasmanian devil)
    Product cytochrome P450 7B1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012541284.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGTTCTT CCTGGCTTCA GTGGCTTTTG AAGTATCTAC TCTGCCTGCC TGGTTTGGCC 
    TTCGCCACCG TTCTACTACT CCTGGCTCTG GGGATAAGAG GGAGAAGGAG GAGGAGACCT 
    GGGGAACCTC CATTGATAAA TGGCTACATT CCTTTTTTTG GAGTGCTCTT TCAGATAAAA 
    AACAACCCCT ACAAAAGCAT CCAAGATTAT CAGAAAAAAT ACGGAAATAT ATTTACCTTA 
    AAAATTAATG GAAACTACTT TACTTTTGTA CTGGATCCAT TTCAATACCC ACTGGTGTTA 
    AAAAATCACA AAAATTTTAC TTTTGAAAAT TTTAAAGATG AATTTATAAA AAAAATTTTC 
    CTGATCTCAA TAAAACCACA CATGAAGAAA GAATTTAAGG ACTCCCAAAA ATATATGCAA 
    AGCAAACTTT TAGATTTGTC TTATACTCTA ATGCAGAATA TGAAGCATAT TATGAAAGAG 
    CGATTTTTAC AAGCCAAAAA TTGGGAAACA AGTCAATTGT ACAAGTTTTG TATATCATTA 
    ATATTTGAAG TCACATATAT AGGCTTCTAT GGAAAGGATC CTGGCACGAA TTCAGGTGAA 
    GTTATCTGTG AATTACTAAA TAACTTTGTC AAATTTGATA ATGAATTCCT GAAACTTTTG 
    TTACCACTAC CTACCTTGTC ACTGAGAACT CTCAAGAATG TTCGGGAAAA GTTAATACAA 
    AATTTTTTAT CAGAGCACAT CTCAAACATG CAGGAAACAT CAGAAATTAT CAAAGCTAGA 
    AAAATCCTTC TTGATAAATT TACAGACCTT CAGGATCATG AAAAAGCAGC ACATCATTTT 
    GCCTTTCTGT GGGCCTCTGT GGCAAACACA ATTCCAGTTA CATTCTGGAC AGTGTATTAT 
    CTTCTGCAGC ACCCAGAAGC TGTTGCAGTG TTGCGTGATG AAATTGACCA TTTGCTACAG 
    TCAACAGGTC AAAAGAAAGA ACCTGATTTT AACATCCAGT TCACCAGAGA ACAATTGGAC 
    AGCTTGGTCT ACCTAGAAAG TATAATTAAT GAGTCCTTTC GAATGACCTC AATTTCTTCC 
    AATGTACGTT ATAGTGAAGA GGATATCACC CTAAATTTAG GAAATGAAGA CGTCAATTTA 
    CGAAAAGGAG ATATCATCAT CATTTACCCA CCATTCCTCC ACTATGACCC AGAAATTTTT 
    GAACAGCCTG AGAAGTTTAA ATTTGATCGT TTTGTAGAAG ATGGCAAGAA GAAAACTACT 
    TTTTTCAAAA GAGGAAAAGA GGTGCCATAT TTCCTATTAC CATTTGGATC TGGAATCAGC 
    AAATGTCCTG GCCGATTCTT AGCAATGCTT GAGATGAAGC AGTTCCTGAT TCTCTTGTTT 
    ATGTATTTTG ATCTAGAATT AATTGAATTT AAACCTCTAG AGTTCAACAA TTCCCGAATT 
    CTTTTTGGTA TACAATGTCC TGAATCAGAT ATTTCCTTTA GATATAAACT AAGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012396738.1
    CDS199..1698
    Translation

    Target ORF information:

    RefSeq Version XM_012541284.3
    Organism Sarcophilus harrisii(Tasmanian devil)
    Definition Sarcophilus harrisii cytochrome P450 7B1 (LOC100920521), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012541284.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGTTCTT CCTGGCTTCA GTGGCTTTTG AAGTATCTAC TCTGCCTGCC TGGTTTGGCC 
    TTCGCCACCG TTCTACTACT CCTGGCTCTG GGGATAAGAG GGAGAAGGAG GAGGAGACCT 
    GGGGAACCTC CATTGATAAA TGGCTACATT CCTTTTTTTG GAGTGCTCTT TCAGATAAAA 
    AACAACCCCT ACAAAAGCAT CCAAGATTAT CAGAAAAAAT ACGGAAATAT ATTTACCTTA 
    AAAATTAATG GAAACTACTT TACTTTTGTA CTGGATCCAT TTCAATACCC ACTGGTGTTA 
    AAAAATCACA AAAATTTTAC TTTTGAAAAT TTTAAAGATG AATTTATAAA AAAAATTTTC 
    CTGATCTCAA TAAAACCACA CATGAAGAAA GAATTTAAGG ACTCCCAAAA ATATATGCAA 
    AGCAAACTTT TAGATTTGTC TTATACTCTA ATGCAGAATA TGAAGCATAT TATGAAAGAG 
    CGATTTTTAC AAGCCAAAAA TTGGGAAACA AGTCAATTGT ACAAGTTTTG TATATCATTA 
    ATATTTGAAG TCACATATAT AGGCTTCTAT GGAAAGGATC CTGGCACGAA TTCAGGTGAA 
    GTTATCTGTG AATTACTAAA TAACTTTGTC AAATTTGATA ATGAATTCCT GAAACTTTTG 
    TTACCACTAC CTACCTTGTC ACTGAGAACT CTCAAGAATG TTCGGGAAAA GTTAATACAA 
    AATTTTTTAT CAGAGCACAT CTCAAACATG CAGGAAACAT CAGAAATTAT CAAAGCTAGA 
    AAAATCCTTC TTGATAAATT TACAGACCTT CAGGATCATG AAAAAGCAGC ACATCATTTT 
    GCCTTTCTGT GGGCCTCTGT GGCAAACACA ATTCCAGTTA CATTCTGGAC AGTGTATTAT 
    CTTCTGCAGC ACCCAGAAGC TGTTGCAGTG TTGCGTGATG AAATTGACCA TTTGCTACAG 
    TCAACAGGTC AAAAGAAAGA ACCTGATTTT AACATCCAGT TCACCAGAGA ACAATTGGAC 
    AGCTTGGTCT ACCTAGAAAG TATAATTAAT GAGTCCTTTC GAATGACCTC AATTTCTTCC 
    AATGTACGTT ATAGTGAAGA GGATATCACC CTAAATTTAG GAAATGAAGA CGTCAATTTA 
    CGAAAAGGAG ATATCATCAT CATTTACCCA CCATTCCTCC ACTATGACCC AGAAATTTTT 
    GAACAGCCTG AGAAGTTTAA ATTTGATCGT TTTGTAGAAG ATGGCAAGAA GAAAACTACT 
    TTTTTCAAAA GAGGAAAAGA GGTGCCATAT TTCCTATTAC CATTTGGATC TGGAATCAGC 
    AAATGTCCTG GCCGATTCTT AGCAATGCTT GAGATGAAGC AGTTCCTGAT TCTCTTGTTT 
    ATGTATTTTG ATCTAGAATT AATTGAATTT AAACCTCTAG AGTTCAACAA TTCCCGAATT 
    CTTTTTGGTA TACAATGTCC TGAATCAGAT ATTTCCTTTA GATATAAACT AAGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100920521 ( XM_012541284.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012396738.1 Sarcophilus harrisii 25-hydroxycholesterol 7-alpha-hydroxylase (LOC100920521), mRNA.
    Price & Availability↑
    CloneID OSa121356
    Clone ID Related Accession (Same CDS sequence) XM_012541284.3 , XM_012541284.1 , XM_012541284.2
    Accession Version XM_012541284.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1431532800000
    Organism Sarcophilus harrisii(Tasmanian devil)
    Product 25-hydroxycholesterol 7-alpha-hydroxylase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823258.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGTTCTT CCTGGCTTCA GTGGCTTTTG AAGTATCTAC TCTGCCTGCC TGGTTTGGCC 
    TTCGCCACCG TTCTACTACT CCTGGCTCTG GGGATAAGAG GGAGAAGGAG GAGGAGACCT 
    GGGGAACCTC CATTGATAAA TGGCTACATT CCTTTTTTTG GAGTGCTCTT TCAGATAAAA 
    AACAACCCCT ACAAAAGCAT CCAAGATTAT CAGAAAAAAT ACGGAAATAT ATTTACCTTA 
    AAAATTAATG GAAACTACTT TACTTTTGTA CTGGATCCAT TTCAATACCC ACTGGTGTTA 
    AAAAATCACA AAAATTTTAC TTTTGAAAAT TTTAAAGATG AATTTATAAA AAAAATTTTC 
    CTGATCTCAA TAAAACCACA CATGAAGAAA GAATTTAAGG ACTCCCAAAA ATATATGCAA 
    AGCAAACTTT TAGATTTGTC TTATACTCTA ATGCAGAATA TGAAGCATAT TATGAAAGAG 
    CGATTTTTAC AAGCCAAAAA TTGGGAAACA AGTCAATTGT ACAAGTTTTG TATATCATTA 
    ATATTTGAAG TCACATATAT AGGCTTCTAT GGAAAGGATC CTGGCACGAA TTCAGGTGAA 
    GTTATCTGTG AATTACTAAA TAACTTTGTC AAATTTGATA ATGAATTCCT GAAACTTTTG 
    TTACCACTAC CTACCTTGTC ACTGAGAACT CTCAAGAATG TTCGGGAAAA GTTAATACAA 
    AATTTTTTAT CAGAGCACAT CTCAAACATG CAGGAAACAT CAGAAATTAT CAAAGCTAGA 
    AAAATCCTTC TTGATAAATT TACAGACCTT CAGGATCATG AAAAAGCAGC ACATCATTTT 
    GCCTTTCTGT GGGCCTCTGT GGCAAACACA ATTCCAGTTA CATTCTGGAC AGTGTATTAT 
    CTTCTGCAGC ACCCAGAAGC TGTTGCAGTG TTGCGTGATG AAATTGACCA TTTGCTACAG 
    TCAACAGGTC AAAAGAAAGA ACCTGATTTT AACATCCAGT TCACCAGAGA ACAATTGGAC 
    AGCTTGGTCT ACCTAGAAAG TATAATTAAT GAGTCCTTTC GAATGACCTC AATTTCTTCC 
    AATGTACGTT ATAGTGAAGA GGATATCACC CTAAATTTAG GAAATGAAGA CGTCAATTTA 
    CGAAAAGGAG ATATCATCAT CATTTACCCA CCATTCCTCC ACTATGACCC AGAAATTTTT 
    GAACAGCCTG AGAAGTTTAA ATTTGATCGT TTTGTAGAAG ATGGCAAGAA GAAAACTACT 
    TTTTTCAAAA GAGGAAAAGA GGTGCCATAT TTCCTATTAC CATTTGGATC TGGAATCAGC 
    AAATGTCCTG GCCGATTCTT AGCAATGCTT GAGATGAAGC AGTTCCTGAT TCTCTTGTTT 
    ATGTATTTTG ATCTAGAATT AATTGAATTT AAACCTCTAG AGTTCAACAA TTCCCGAATT 
    CTTTTTGGTA TACAATGTCC TGAATCAGAT ATTTCCTTTA GATATAAACT AAGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012396738.1
    CDS96..1595
    Translation

    Target ORF information:

    RefSeq Version XM_012541284.1
    Organism Sarcophilus harrisii(Tasmanian devil)
    Definition Sarcophilus harrisii 25-hydroxycholesterol 7-alpha-hydroxylase (LOC100920521), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012541284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGTTCTT CCTGGCTTCA GTGGCTTTTG AAGTATCTAC TCTGCCTGCC TGGTTTGGCC 
    TTCGCCACCG TTCTACTACT CCTGGCTCTG GGGATAAGAG GGAGAAGGAG GAGGAGACCT 
    GGGGAACCTC CATTGATAAA TGGCTACATT CCTTTTTTTG GAGTGCTCTT TCAGATAAAA 
    AACAACCCCT ACAAAAGCAT CCAAGATTAT CAGAAAAAAT ACGGAAATAT ATTTACCTTA 
    AAAATTAATG GAAACTACTT TACTTTTGTA CTGGATCCAT TTCAATACCC ACTGGTGTTA 
    AAAAATCACA AAAATTTTAC TTTTGAAAAT TTTAAAGATG AATTTATAAA AAAAATTTTC 
    CTGATCTCAA TAAAACCACA CATGAAGAAA GAATTTAAGG ACTCCCAAAA ATATATGCAA 
    AGCAAACTTT TAGATTTGTC TTATACTCTA ATGCAGAATA TGAAGCATAT TATGAAAGAG 
    CGATTTTTAC AAGCCAAAAA TTGGGAAACA AGTCAATTGT ACAAGTTTTG TATATCATTA 
    ATATTTGAAG TCACATATAT AGGCTTCTAT GGAAAGGATC CTGGCACGAA TTCAGGTGAA 
    GTTATCTGTG AATTACTAAA TAACTTTGTC AAATTTGATA ATGAATTCCT GAAACTTTTG 
    TTACCACTAC CTACCTTGTC ACTGAGAACT CTCAAGAATG TTCGGGAAAA GTTAATACAA 
    AATTTTTTAT CAGAGCACAT CTCAAACATG CAGGAAACAT CAGAAATTAT CAAAGCTAGA 
    AAAATCCTTC TTGATAAATT TACAGACCTT CAGGATCATG AAAAAGCAGC ACATCATTTT 
    GCCTTTCTGT GGGCCTCTGT GGCAAACACA ATTCCAGTTA CATTCTGGAC AGTGTATTAT 
    CTTCTGCAGC ACCCAGAAGC TGTTGCAGTG TTGCGTGATG AAATTGACCA TTTGCTACAG 
    TCAACAGGTC AAAAGAAAGA ACCTGATTTT AACATCCAGT TCACCAGAGA ACAATTGGAC 
    AGCTTGGTCT ACCTAGAAAG TATAATTAAT GAGTCCTTTC GAATGACCTC AATTTCTTCC 
    AATGTACGTT ATAGTGAAGA GGATATCACC CTAAATTTAG GAAATGAAGA CGTCAATTTA 
    CGAAAAGGAG ATATCATCAT CATTTACCCA CCATTCCTCC ACTATGACCC AGAAATTTTT 
    GAACAGCCTG AGAAGTTTAA ATTTGATCGT TTTGTAGAAG ATGGCAAGAA GAAAACTACT 
    TTTTTCAAAA GAGGAAAAGA GGTGCCATAT TTCCTATTAC CATTTGGATC TGGAATCAGC 
    AAATGTCCTG GCCGATTCTT AGCAATGCTT GAGATGAAGC AGTTCCTGAT TCTCTTGTTT 
    ATGTATTTTG ATCTAGAATT AATTGAATTT AAACCTCTAG AGTTCAACAA TTCCCGAATT 
    CTTTTTGGTA TACAATGTCC TGAATCAGAT ATTTCCTTTA GATATAAACT AAGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100920521 ( XM_012541284.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012396738.1 Sarcophilus harrisii 25-hydroxycholesterol 7-alpha-hydroxylase (LOC100920521), mRNA.
    Price & Availability↑
    CloneID OSa121356
    Clone ID Related Accession (Same CDS sequence) XM_012541284.3 , XM_012541284.1 , XM_012541284.2
    Accession Version XM_012541284.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1515513600000
    Organism Sarcophilus harrisii(Tasmanian devil)
    Product 25-hydroxycholesterol 7-alpha-hydroxylase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823258.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012541284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGTTCTT CCTGGCTTCA GTGGCTTTTG AAGTATCTAC TCTGCCTGCC TGGTTTGGCC 
    TTCGCCACCG TTCTACTACT CCTGGCTCTG GGGATAAGAG GGAGAAGGAG GAGGAGACCT 
    GGGGAACCTC CATTGATAAA TGGCTACATT CCTTTTTTTG GAGTGCTCTT TCAGATAAAA 
    AACAACCCCT ACAAAAGCAT CCAAGATTAT CAGAAAAAAT ACGGAAATAT ATTTACCTTA 
    AAAATTAATG GAAACTACTT TACTTTTGTA CTGGATCCAT TTCAATACCC ACTGGTGTTA 
    AAAAATCACA AAAATTTTAC TTTTGAAAAT TTTAAAGATG AATTTATAAA AAAAATTTTC 
    CTGATCTCAA TAAAACCACA CATGAAGAAA GAATTTAAGG ACTCCCAAAA ATATATGCAA 
    AGCAAACTTT TAGATTTGTC TTATACTCTA ATGCAGAATA TGAAGCATAT TATGAAAGAG 
    CGATTTTTAC AAGCCAAAAA TTGGGAAACA AGTCAATTGT ACAAGTTTTG TATATCATTA 
    ATATTTGAAG TCACATATAT AGGCTTCTAT GGAAAGGATC CTGGCACGAA TTCAGGTGAA 
    GTTATCTGTG AATTACTAAA TAACTTTGTC AAATTTGATA ATGAATTCCT GAAACTTTTG 
    TTACCACTAC CTACCTTGTC ACTGAGAACT CTCAAGAATG TTCGGGAAAA GTTAATACAA 
    AATTTTTTAT CAGAGCACAT CTCAAACATG CAGGAAACAT CAGAAATTAT CAAAGCTAGA 
    AAAATCCTTC TTGATAAATT TACAGACCTT CAGGATCATG AAAAAGCAGC ACATCATTTT 
    GCCTTTCTGT GGGCCTCTGT GGCAAACACA ATTCCAGTTA CATTCTGGAC AGTGTATTAT 
    CTTCTGCAGC ACCCAGAAGC TGTTGCAGTG TTGCGTGATG AAATTGACCA TTTGCTACAG 
    TCAACAGGTC AAAAGAAAGA ACCTGATTTT AACATCCAGT TCACCAGAGA ACAATTGGAC 
    AGCTTGGTCT ACCTAGAAAG TATAATTAAT GAGTCCTTTC GAATGACCTC AATTTCTTCC 
    AATGTACGTT ATAGTGAAGA GGATATCACC CTAAATTTAG GAAATGAAGA CGTCAATTTA 
    CGAAAAGGAG ATATCATCAT CATTTACCCA CCATTCCTCC ACTATGACCC AGAAATTTTT 
    GAACAGCCTG AGAAGTTTAA ATTTGATCGT TTTGTAGAAG ATGGCAAGAA GAAAACTACT 
    TTTTTCAAAA GAGGAAAAGA GGTGCCATAT TTCCTATTAC CATTTGGATC TGGAATCAGC 
    AAATGTCCTG GCCGATTCTT AGCAATGCTT GAGATGAAGC AGTTCCTGAT TCTCTTGTTT 
    ATGTATTTTG ATCTAGAATT AATTGAATTT AAACCTCTAG AGTTCAACAA TTCCCGAATT 
    CTTTTTGGTA TACAATGTCC TGAATCAGAT ATTTCCTTTA GATATAAACT AAGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012396738.1
    CDS139..1638
    Translation

    Target ORF information:

    RefSeq Version XM_012541284.2
    Organism Sarcophilus harrisii(Tasmanian devil)
    Definition Sarcophilus harrisii 25-hydroxycholesterol 7-alpha-hydroxylase (LOC100920521), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012541284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGTTCTT CCTGGCTTCA GTGGCTTTTG AAGTATCTAC TCTGCCTGCC TGGTTTGGCC 
    TTCGCCACCG TTCTACTACT CCTGGCTCTG GGGATAAGAG GGAGAAGGAG GAGGAGACCT 
    GGGGAACCTC CATTGATAAA TGGCTACATT CCTTTTTTTG GAGTGCTCTT TCAGATAAAA 
    AACAACCCCT ACAAAAGCAT CCAAGATTAT CAGAAAAAAT ACGGAAATAT ATTTACCTTA 
    AAAATTAATG GAAACTACTT TACTTTTGTA CTGGATCCAT TTCAATACCC ACTGGTGTTA 
    AAAAATCACA AAAATTTTAC TTTTGAAAAT TTTAAAGATG AATTTATAAA AAAAATTTTC 
    CTGATCTCAA TAAAACCACA CATGAAGAAA GAATTTAAGG ACTCCCAAAA ATATATGCAA 
    AGCAAACTTT TAGATTTGTC TTATACTCTA ATGCAGAATA TGAAGCATAT TATGAAAGAG 
    CGATTTTTAC AAGCCAAAAA TTGGGAAACA AGTCAATTGT ACAAGTTTTG TATATCATTA 
    ATATTTGAAG TCACATATAT AGGCTTCTAT GGAAAGGATC CTGGCACGAA TTCAGGTGAA 
    GTTATCTGTG AATTACTAAA TAACTTTGTC AAATTTGATA ATGAATTCCT GAAACTTTTG 
    TTACCACTAC CTACCTTGTC ACTGAGAACT CTCAAGAATG TTCGGGAAAA GTTAATACAA 
    AATTTTTTAT CAGAGCACAT CTCAAACATG CAGGAAACAT CAGAAATTAT CAAAGCTAGA 
    AAAATCCTTC TTGATAAATT TACAGACCTT CAGGATCATG AAAAAGCAGC ACATCATTTT 
    GCCTTTCTGT GGGCCTCTGT GGCAAACACA ATTCCAGTTA CATTCTGGAC AGTGTATTAT 
    CTTCTGCAGC ACCCAGAAGC TGTTGCAGTG TTGCGTGATG AAATTGACCA TTTGCTACAG 
    TCAACAGGTC AAAAGAAAGA ACCTGATTTT AACATCCAGT TCACCAGAGA ACAATTGGAC 
    AGCTTGGTCT ACCTAGAAAG TATAATTAAT GAGTCCTTTC GAATGACCTC AATTTCTTCC 
    AATGTACGTT ATAGTGAAGA GGATATCACC CTAAATTTAG GAAATGAAGA CGTCAATTTA 
    CGAAAAGGAG ATATCATCAT CATTTACCCA CCATTCCTCC ACTATGACCC AGAAATTTTT 
    GAACAGCCTG AGAAGTTTAA ATTTGATCGT TTTGTAGAAG ATGGCAAGAA GAAAACTACT 
    TTTTTCAAAA GAGGAAAAGA GGTGCCATAT TTCCTATTAC CATTTGGATC TGGAATCAGC 
    AAATGTCCTG GCCGATTCTT AGCAATGCTT GAGATGAAGC AGTTCCTGAT TCTCTTGTTT 
    ATGTATTTTG ATCTAGAATT AATTGAATTT AAACCTCTAG AGTTCAACAA TTCCCGAATT 
    CTTTTTGGTA TACAATGTCC TGAATCAGAT ATTTCCTTTA GATATAAACT AAGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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