HECW2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2
Entrez Gene ID	100919405
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031960290.1	XP_031816150.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_031960291.1	XP_031816151.1	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_031960289.1	XP_031816149.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_003764075.2	XP_003764123.1	E3 ubiquitin-protein ligase HECW2

Pathways from BioSystems
Gene Ontology

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa125886	XM_003764075.2 Latest version!	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa229933	XM_031960291.1 Latest version!	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa229931	XM_031960289.1 Latest version!	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa229932	XM_031960290.1 Latest version!	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2 ( XM_003764075.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003764123.1 Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Price & Availability↑

CloneID	OSa125886
Clone ID Related Accession (Same CDS sequence)	XM_003764075.2
Accession Version	XM_003764075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003764075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCTAGCT CAGCCAGAGA GCATCTGCTT TTTGTGAGGA GGCGCAACCC CCAGATGCGC 
TACACACTGA GTCCAGAGAA CCTTCAGAGT CTCGCCTCTC AGGGTTCCAT GCCCGAGAAC 
ATGAACCTTC AGAGGGCTAA TAGTGACACT GATCTGGTGA CTTCGGAAAG CCGCTCTAGC 
CTGACTGCAA GCATGTATGA ATATACCCTG GGCCAAGCCC AGAACCTCAT TATCTTCTGG 
GATATTAAAG AGGAAGTGGA CCCTACTGAC TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTTTGGGA TTCCAAAAAC CGAGGTGTTA CTGGGACACA AAAAGGACAA 
ATTGTTTGGA GAATTGAACC TGGGCCCTAT TTTATGGAAC ATCTTAGGGC AGTTGGGCTA 
AAGAAAGGAA TGTTCTTTAA TCCTGATCCT TACCTTAAGA TGTCAATTCA GCCAGGAAAG 
AAGAGCAGTT TTCCTACATT TGCCCATCAT GGGCAGGAAC GAAGGTCTAC CATCATCAGT 
AACACAACTA ATCCAATTTG GCACAGAGAG AAATACTCTT TTTTTGCACT TCTTACTGAT 
GTCTTAGAAA TTGAAATTAA AGACAAATTT GCCAAGAGTC GTCCTATCAT CAAGCGTTTT 
CTTGGGAAAC TAACCATACC AGTCCAGAGG TTGCTGGAGC GTCAAGCCAT TGGTGATCAA 
ATGCTCAGCT ACAACCTTGG CAGAAGGCTC CCAGCTGACC ATGTGAGTGG GTATCTCCAA 
TTCAAAGTTG AGATTACATC TTCTATTCAT GAAGATGCTT CCCCAGAAGC AGTTGGTACC 
ATCCTTGGAG TAAATTCTGT GAACGGAGAC CTGGGGAGTC CCTCTGATGA TGAGGATATG 
CCGGGGGCAC ATCACGACTC CCCTGCAGTT TCTAATGGTC CAGTTTCTGA GGAAAGTGAT 
GGCACTCCCA AGCATTCCTT TAGGACTAGC TCTACTATGG AAATAGACCC TGAGGACCTC 
ACTTCTAGTT CTTCCAGGAC CTCCCCTCCC AGAGGACGGC AGGATTCGCT GAACGATTAT 
CTGGATGCCA TTGAGCACAA TGGTCATTCC AGGCCGGGGA CATCCGCCTG TGCTGAGAGG 
TCCCTGGGAG CTTCTCCCAA GCTGAGGAGC AGCTTCCCCA CCGATACCAG ACTCAATGCC 
ATGCTTCACA TTGACTCTGA TGAGGAGGAT CATGAGTTTC AGCAAGATCT AGGTTATCCC 
TCTTCCTTGG AAGAGGAAGG GGGGCTAGTG ATGTTCAGTA GTGCTTCACG GACTGAGGAG 
GGGAACTCGG CAAGTCCGGC AAAGCCAGAA TCCAGCCCCG CTGAGGGGGA GGAACCCCCA 
GCAACTGAAG CCGCTTCTGT GGAGAATAAG CAGGAGGAGG AACTTGCGGA GGTCCCTGAC 
AGTTCCTCAG TTTCAGATGC AGCTGCCGAA CGAGAAAATG GCCAGGAACC TCAGCCAAGT 
GAAGAGCAAG GCAATGGTGA ATTGGGTGAC TCTCAAGAGG GTGACCCCCC TACCAGCGAG 
GCAGACATGG GAAATTTGGA TCTGCCTGGG GAAAGCAGAA GGGCCATTAG TGAAACTGAG 
TCGATTGACC AAGGGTCTGA GCCTTCCCAG GTATCATCTG AGACAGAACC CAGTGACCCC 
GCCCGGACCG AGAGCGTCAG CGAAGCCAGC ACCAGGCCCG AGGGGGAGAG CGACGCCGAA 
GGGGCCGATA GTTCCTGCAA CGAGAGTGTT ACCACGCAGC TGTCTTCGGT GGATACCCGC 
TGCTCCTCTC TGGAGAGTGC CCGGTTTCCT GAAACTCCAG CTTTTTCGTC TCAGGAGGAA 
GAGGAGGGGG CTTGTGCTTC AGAACCTGCC CATGGTGCCA CTGCTGCAGG GTCTCAGGAC 
TCCGTGTGCA CCCCTGGGTC TTTGCCCATG GTAAGGGTAC CCAGCGGTCA GGAGGAGGGA 
CAGGTGGCAG ATGGGCCTGC GTTGGCTGAT GGAGAGGAGA TGGGCGAGAT CTGGCAGAGG 
AGGAATAGTC TTCAGGCTAC GGCAGAGAGC CAGGCCCACG AAGAAGATGC TGGGGATGCT 
CAAAACGCTT GTGAAGGGGC CGCAGCTCAA GCTGAAGGAG CTACTGGAGG TTCTCAAGCC 
AACGGCCACC AGCCATTGCG ATCGCTACCT TCAGTACGTC AGGATGTTAG CCGATACCAG 
AGGGTGGACG AGGCTCTGCC ACCAAATTGG GAGGCTCGGA TTGACAGCCA TGGAAGGATA 
TTCTATGTGG ACCATGTAAA CAGAACAACA ACGTGGCAGA GGCCCACTGC TCCCCCCGCC 
CCACAGATAC TTCAGAGATC CAATTCTATA CAACAAATGG AACAGCTAAA CCGTAGGTAT 
CAAAGTATCC GCAGGACAAT GACTAATGAA AGGCCTGAAG AACACACTAA TGCTATTGAT 
GGAGCTGGAG AGGACACAGA CTTCCATCAG TCCAATGCAG ATTTTCGACG GGAGAATGTG 
CTGCCCCACT CTACCTCCAG GTCCAGGCTG ACGTTACTGT TACAGTCTCC CCCCGTGAAA 
TTTCTCATAA GCCCAGAGTT CTTCACCGTG CTGCATTCTA ACCCTAGTGC CTATCGCATG 
TTTACAAACA ATACGTGTTT GAAGCACATG ATCACCAAAG TCCGGCGGGA CACTCACCAC 
TTTGAACGCT ACCAGCATAA TCGAGACCTA GTGGGATTCC TCAACATGTT CGCTAACAAA 
CAGCTGGAGC TGCCACGGGG CTGGGAAATG AAGCATGATC ACCAGGGCAA GGCTTTTTTT 
GTCGACCACA ATTCCCGTAC CACCACCTTC ATTGACCCTC GGCTCCCACT TCAGAGCAGC 
AGACCAACAA GCGCTTTGGT ACATCGGCAA CATCTGACCA GACAGCGCAG TCATAGTGCT 
GGTGAAGTAG GAGAGGACTC TCGACATTCA GGGCCACCAG TTCTTCCTAG GCCATCCAGC 
ACCTTCAATA CAGTCAGTAG GTCACAGTAC CAGGATATGG TTCCAGTAGC CATGAGGGAG 
AAGATTCAGT TTATCCGGAC GGAGGGGACA CCTGGACTGG TACGTCTTTC CAGTGATGCT 
GATCTTGTGA TGTTGCTAAG TTTATTTGAA GAAGAGATAA TGTCATATGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA TTGCCAGTCC CCACGCAGTT CCCCTGTGTC CTCGCCTCAG 
AACTCTCCAG GTACGCAGCG TGCCAATGCT CGAGCTCCCG CCCCTTATAA ACGAGATTTT 
GAAGCCAAAC TACGGAATTT CTACAGGAAG TTGGAAACTA AAGGATATGG GCAAGGGCCC 
GGGAAGTTAA AGTTAATCAT CAGAAGAGAC CATTTGTTGG AAGATGCTTT TAATCAGATT 
ATGGGCTATT CCAGAAAAGA CTTGCAGAGG AATAAGCTCT ATGTCACCTT TGTTGGAGAG 
GAAGGGCTGG ATTACAGTGG ACCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAATCCAT ATTATGGCTT ATTTGAATAT TCAGCTAATG ATACATATAC AGTACAAATA 
AGTCCCATGT CTGCTTTTGT AGACAATCAC CACGAATGGT TCAGGTTTAG TGGTCGAATC 
CTTGGCCTTG CACTGATACA CCAGTATTTG TTAGATGCCT TCTTCACACG ACCATTTTAT 
AAGGCACTCC TCAGAATTCT GTGTGATTTG AGTGATCTAG AATACCTTGA TGAGGAGTTC 
CACCAGAGCC TTCAGTGGAT GAAAGACAAT GACATACATG ATATCCTAGA CCTCACCTTT 
ACTGTAAATG AAGAAGTTTT TGGACAGATC ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TCACAGAGAA GAACAAGAAA GAATACATCG AAAGAATGGT GAAATGGAGA 
ATTGAGAGGG GTGTTGTACA ACAAACAGAG AGCTTAGTAC GAGGTTTTTA TGAGGTAGTG 
GATGCTAGAC TGGTGTCTGT CTTTGATGCA AGAGAACTGG AACTGGTCAT TGCTGGAACT 
GCTGAAATAG ATTTAAGTGA CTGGAGAAAC AACACAGAAT ATAGAGGAGG ATATCATGAC 
AATCATATTG TAATCCGGTG GTTCTGGGCT GCTGTAGAAA GATTTAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT CACAGGCACA TCCAGCATAC CCTATGAAGG CTTTGCTTCT 
CTGCGAGGGA GTAATGGGCC AAGGAGATTC TGTGTGGAGA AATGGGGAAA AATCACTGCC 
CTTCCCAGGG CTCACACTTG TTTTAATCGT CTGGACCTTC CCCCCTATCC ATCATTCTCC 
ATGCTTTATG AAAAACTTTT GACAGCTGTA GAAGAAACAA GTACTTTTGG ACTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003764123.1
CDS	1..4440
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLASQGSMPENMNLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEHLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTFAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGAHHDSPAVSNGPVSEESDGTPKHSFRTSSTMEIDPEDLTSSSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTSACAERSLGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLVMFSSASRTEEGNSASPAKPESSPAEGEEPPATEAASVENKQEEELAEVPDSSSVSDAAAERENGQEPQPSEEQGNGELGDSQEGDPPTSEADMGNLDLPGESRRAISETESIDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDAEGADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEEGACASEPAHGATAAGSQDSVCTPGSLPMVRVPSGQEEGQVADGPALADGEEMGEIWQRRNSLQATAESQAHEEDAGDAQNACEGAAAQAEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNERPEEHTNAIDGAGEDTDFHQSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRSQYQDMVPVAMREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRSSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003764075.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003764075.2

ORF Insert Sequence:

ATGGCTAGCT CAGCCAGAGA GCATCTGCTT TTTGTGAGGA GGCGCAACCC CCAGATGCGC 
TACACACTGA GTCCAGAGAA CCTTCAGAGT CTCGCCTCTC AGGGTTCCAT GCCCGAGAAC 
ATGAACCTTC AGAGGGCTAA TAGTGACACT GATCTGGTGA CTTCGGAAAG CCGCTCTAGC 
CTGACTGCAA GCATGTATGA ATATACCCTG GGCCAAGCCC AGAACCTCAT TATCTTCTGG 
GATATTAAAG AGGAAGTGGA CCCTACTGAC TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTTTGGGA TTCCAAAAAC CGAGGTGTTA CTGGGACACA AAAAGGACAA 
ATTGTTTGGA GAATTGAACC TGGGCCCTAT TTTATGGAAC ATCTTAGGGC AGTTGGGCTA 
AAGAAAGGAA TGTTCTTTAA TCCTGATCCT TACCTTAAGA TGTCAATTCA GCCAGGAAAG 
AAGAGCAGTT TTCCTACATT TGCCCATCAT GGGCAGGAAC GAAGGTCTAC CATCATCAGT 
AACACAACTA ATCCAATTTG GCACAGAGAG AAATACTCTT TTTTTGCACT TCTTACTGAT 
GTCTTAGAAA TTGAAATTAA AGACAAATTT GCCAAGAGTC GTCCTATCAT CAAGCGTTTT 
CTTGGGAAAC TAACCATACC AGTCCAGAGG TTGCTGGAGC GTCAAGCCAT TGGTGATCAA 
ATGCTCAGCT ACAACCTTGG CAGAAGGCTC CCAGCTGACC ATGTGAGTGG GTATCTCCAA 
TTCAAAGTTG AGATTACATC TTCTATTCAT GAAGATGCTT CCCCAGAAGC AGTTGGTACC 
ATCCTTGGAG TAAATTCTGT GAACGGAGAC CTGGGGAGTC CCTCTGATGA TGAGGATATG 
CCGGGGGCAC ATCACGACTC CCCTGCAGTT TCTAATGGTC CAGTTTCTGA GGAAAGTGAT 
GGCACTCCCA AGCATTCCTT TAGGACTAGC TCTACTATGG AAATAGACCC TGAGGACCTC 
ACTTCTAGTT CTTCCAGGAC CTCCCCTCCC AGAGGACGGC AGGATTCGCT GAACGATTAT 
CTGGATGCCA TTGAGCACAA TGGTCATTCC AGGCCGGGGA CATCCGCCTG TGCTGAGAGG 
TCCCTGGGAG CTTCTCCCAA GCTGAGGAGC AGCTTCCCCA CCGATACCAG ACTCAATGCC 
ATGCTTCACA TTGACTCTGA TGAGGAGGAT CATGAGTTTC AGCAAGATCT AGGTTATCCC 
TCTTCCTTGG AAGAGGAAGG GGGGCTAGTG ATGTTCAGTA GTGCTTCACG GACTGAGGAG 
GGGAACTCGG CAAGTCCGGC AAAGCCAGAA TCCAGCCCCG CTGAGGGGGA GGAACCCCCA 
GCAACTGAAG CCGCTTCTGT GGAGAATAAG CAGGAGGAGG AACTTGCGGA GGTCCCTGAC 
AGTTCCTCAG TTTCAGATGC AGCTGCCGAA CGAGAAAATG GCCAGGAACC TCAGCCAAGT 
GAAGAGCAAG GCAATGGTGA ATTGGGTGAC TCTCAAGAGG GTGACCCCCC TACCAGCGAG 
GCAGACATGG GAAATTTGGA TCTGCCTGGG GAAAGCAGAA GGGCCATTAG TGAAACTGAG 
TCGATTGACC AAGGGTCTGA GCCTTCCCAG GTATCATCTG AGACAGAACC CAGTGACCCC 
GCCCGGACCG AGAGCGTCAG CGAAGCCAGC ACCAGGCCCG AGGGGGAGAG CGACGCCGAA 
GGGGCCGATA GTTCCTGCAA CGAGAGTGTT ACCACGCAGC TGTCTTCGGT GGATACCCGC 
TGCTCCTCTC TGGAGAGTGC CCGGTTTCCT GAAACTCCAG CTTTTTCGTC TCAGGAGGAA 
GAGGAGGGGG CTTGTGCTTC AGAACCTGCC CATGGTGCCA CTGCTGCAGG GTCTCAGGAC 
TCCGTGTGCA CCCCTGGGTC TTTGCCCATG GTAAGGGTAC CCAGCGGTCA GGAGGAGGGA 
CAGGTGGCAG ATGGGCCTGC GTTGGCTGAT GGAGAGGAGA TGGGCGAGAT CTGGCAGAGG 
AGGAATAGTC TTCAGGCTAC GGCAGAGAGC CAGGCCCACG AAGAAGATGC TGGGGATGCT 
CAAAACGCTT GTGAAGGGGC CGCAGCTCAA GCTGAAGGAG CTACTGGAGG TTCTCAAGCC 
AACGGCCACC AGCCATTGCG ATCGCTACCT TCAGTACGTC AGGATGTTAG CCGATACCAG 
AGGGTGGACG AGGCTCTGCC ACCAAATTGG GAGGCTCGGA TTGACAGCCA TGGAAGGATA 
TTCTATGTGG ACCATGTAAA CAGAACAACA ACGTGGCAGA GGCCCACTGC TCCCCCCGCC 
CCACAGATAC TTCAGAGATC CAATTCTATA CAACAAATGG AACAGCTAAA CCGTAGGTAT 
CAAAGTATCC GCAGGACAAT GACTAATGAA AGGCCTGAAG AACACACTAA TGCTATTGAT 
GGAGCTGGAG AGGACACAGA CTTCCATCAG TCCAATGCAG ATTTTCGACG GGAGAATGTG 
CTGCCCCACT CTACCTCCAG GTCCAGGCTG ACGTTACTGT TACAGTCTCC CCCCGTGAAA 
TTTCTCATAA GCCCAGAGTT CTTCACCGTG CTGCATTCTA ACCCTAGTGC CTATCGCATG 
TTTACAAACA ATACGTGTTT GAAGCACATG ATCACCAAAG TCCGGCGGGA CACTCACCAC 
TTTGAACGCT ACCAGCATAA TCGAGACCTA GTGGGATTCC TCAACATGTT CGCTAACAAA 
CAGCTGGAGC TGCCACGGGG CTGGGAAATG AAGCATGATC ACCAGGGCAA GGCTTTTTTT 
GTCGACCACA ATTCCCGTAC CACCACCTTC ATTGACCCTC GGCTCCCACT TCAGAGCAGC 
AGACCAACAA GCGCTTTGGT ACATCGGCAA CATCTGACCA GACAGCGCAG TCATAGTGCT 
GGTGAAGTAG GAGAGGACTC TCGACATTCA GGGCCACCAG TTCTTCCTAG GCCATCCAGC 
ACCTTCAATA CAGTCAGTAG GTCACAGTAC CAGGATATGG TTCCAGTAGC CATGAGGGAG 
AAGATTCAGT TTATCCGGAC GGAGGGGACA CCTGGACTGG TACGTCTTTC CAGTGATGCT 
GATCTTGTGA TGTTGCTAAG TTTATTTGAA GAAGAGATAA TGTCATATGT GCCTCCACAT 
GCCTTACTCC ACCCCAGCTA TTGCCAGTCC CCACGCAGTT CCCCTGTGTC CTCGCCTCAG 
AACTCTCCAG GTACGCAGCG TGCCAATGCT CGAGCTCCCG CCCCTTATAA ACGAGATTTT 
GAAGCCAAAC TACGGAATTT CTACAGGAAG TTGGAAACTA AAGGATATGG GCAAGGGCCC 
GGGAAGTTAA AGTTAATCAT CAGAAGAGAC CATTTGTTGG AAGATGCTTT TAATCAGATT 
ATGGGCTATT CCAGAAAAGA CTTGCAGAGG AATAAGCTCT ATGTCACCTT TGTTGGAGAG 
GAAGGGCTGG ATTACAGTGG ACCTTCTAGA GAGTTTTTCT TCCTGGTATC CAGAGAACTC 
TTTAATCCAT ATTATGGCTT ATTTGAATAT TCAGCTAATG ATACATATAC AGTACAAATA 
AGTCCCATGT CTGCTTTTGT AGACAATCAC CACGAATGGT TCAGGTTTAG TGGTCGAATC 
CTTGGCCTTG CACTGATACA CCAGTATTTG TTAGATGCCT TCTTCACACG ACCATTTTAT 
AAGGCACTCC TCAGAATTCT GTGTGATTTG AGTGATCTAG AATACCTTGA TGAGGAGTTC 
CACCAGAGCC TTCAGTGGAT GAAAGACAAT GACATACATG ATATCCTAGA CCTCACCTTT 
ACTGTAAATG AAGAAGTTTT TGGACAGATC ACTGAACGAG AATTAAAGCC AGGGGGTGCC 
AATATCCCAG TCACAGAGAA GAACAAGAAA GAATACATCG AAAGAATGGT GAAATGGAGA 
ATTGAGAGGG GTGTTGTACA ACAAACAGAG AGCTTAGTAC GAGGTTTTTA TGAGGTAGTG 
GATGCTAGAC TGGTGTCTGT CTTTGATGCA AGAGAACTGG AACTGGTCAT TGCTGGAACT 
GCTGAAATAG ATTTAAGTGA CTGGAGAAAC AACACAGAAT ATAGAGGAGG ATATCATGAC 
AATCATATTG TAATCCGGTG GTTCTGGGCT GCTGTAGAAA GATTTAACAA TGAACAACGA 
CTAAGGTTGT TACAGTTTGT CACAGGCACA TCCAGCATAC CCTATGAAGG CTTTGCTTCT 
CTGCGAGGGA GTAATGGGCC AAGGAGATTC TGTGTGGAGA AATGGGGAAA AATCACTGCC 
CTTCCCAGGG CTCACACTTG TTTTAATCGT CTGGACCTTC CCCCCTATCC ATCATTCTCC 
ATGCTTTATG AAAAACTTTT GACAGCTGTA GAAGAAACAA GTACTTTTGG ACTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_031960291.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031816151.1 Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSa229933
Clone ID Related Accession (Same CDS sequence)	XM_031960291.1
Accession Version	XM_031960291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AAATAAAAAT ATGTTTTAAA TACTACCATG GCATTAGTGG AGCCTTGAGA 
GCTACTACCC CATGTATTAC TGTGAAAAAT CCTGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGTG CTTCAGGAAA TCAACATTGT CGGAAATTAG TCAGTTTTAC ATTGTCTGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGAATG TTCTTTAATC CTGATCCTTA CCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTT CCTACATTTG CCCATCATGG GCAGGAACGA 
AGGTCTACCA TCATCAGTAA CACAACTAAT CCAATTTGGC ACAGAGAGAA ATACTCTTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGTCGT 
CCTATCATCA AGCGTTTTCT TGGGAAACTA ACCATACCAG TCCAGAGGTT GCTGGAGCGT 
CAAGCCATTG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT 
GTGAGTGGGT ATCTCCAATT CAAAGTTGAG ATTACATCTT CTATTCATGA AGATGCTTCC 
CCAGAAGCAG TTGGTACCAT CCTTGGAGTA AATTCTGTGA ACGGAGACCT GGGGAGTCCC 
TCTGATGATG AGGATATGCC GGGGGCACAT CACGACTCCC CTGCAGTTTC TAATGGTCCA 
GTTTCTGAGG AAAGTGATGG CACTCCCAAG CATTCCTTTA GGACTAGCTC TACTATGGAA 
ATAGACCCTG AGGACCTCAC TTCTAGTTCT TCCAGGACCT CCCCTCCCAG AGGACGGCAG 
GATTCGCTGA ACGATTATCT GGATGCCATT GAGCACAATG GTCATTCCAG GCCGGGGACA 
TCCGCCTGTG CTGAGAGGTC CCTGGGAGCT TCTCCCAAGC TGAGGAGCAG CTTCCCCACC 
GATACCAGAC TCAATGCCAT GCTTCACATT GACTCTGATG AGGAGGATCA TGAGTTTCAG 
CAAGATCTAG GTTATCCCTC TTCCTTGGAA GAGGAAGGGG GGCTAGTGAT GTTCAGTAGT 
GCTTCACGGA CTGAGGAGGG GAACTCGGCA AGTCCGGCAA AGCCAGAATC CAGCCCCGCT 
GAGGGGGAGG AACCCCCAGC AACTGAAGCC GCTTCTGTGG AGAATAAGCA GGAGGAGGAA 
CTTGCGGAGG TCCCTGACAG TTCCTCAGTT TCAGATGCAG CTGCCGAACG AGAAAATGGC 
CAGGAACCTC AGCCAAGTGA AGAGCAAGGC AATGGTGAAT TGGGTGACTC TCAAGAGGGT 
GACCCCCCTA CCAGCGAGGC AGACATGGGA AATTTGGATC TGCCTGGGGA AAGCAGAAGG 
GCCATTAGTG AAACTGAGTC GATTGACCAA GGGTCTGAGC CTTCCCAGGT ATCATCTGAG 
ACAGAACCCA GTGACCCCGC CCGGACCGAG AGCGTCAGCG AAGCCAGCAC CAGGCCCGAG 
GGGGAGAGCG ACGCCGAAGG GGCCGATAGT TCCTGCAACG AGAGTGTTAC CACGCAGCTG 
TCTTCGGTGG ATACCCGCTG CTCCTCTCTG GAGAGTGCCC GGTTTCCTGA AACTCCAGCT 
TTTTCGTCTC AGGAGGAAGA GGAGGGGGCT TGTGCTTCAG AACCTGCCCA TGGTGCCACT 
GCTGCAGGGT CTCAGGACTC CGTGTGCACC CCTGGGTCTT TGCCCATGGT AAGGGTACCC 
AGCGGTCAGG AGGAGGGACA GGTGGCAGAT GGGCCTGCGT TGGCTGATGG AGAGGAGATG 
GGCGAGATCT GGCAGAGGAG GAATAGTCTT CAGGCTACGG CAGAGAGCCA GGCCCACGAA 
GAAGATGCTG GGGATGCTCA AAACGCTTGT GAAGGGGCCG CAGCTCAAGC TGAAGGAGCT 
ACTGGAGGTT CTCAAGCCAA CGGCCACCAG CCATTGCGAT CGCTACCTTC AGTACGTCAG 
GATGTTAGCC GATACCAGAG GGTGGACGAG GCTCTGCCAC CAAATTGGGA GGCTCGGATT 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACAACAAC GTGGCAGAGG 
CCCACTGCTC CCCCCGCCCC ACAGATACTT CAGAGATCCA ATTCTATACA ACAAATGGAA 
CAGCTAAACC GTAGGTATCA AAGTATCCGC AGGACAATGA CTAATGAAAG GCCTGAAGAA 
CACACTAATG CTATTGATGG AGCTGGAGAG GACACAGACT TCCATCAGTC CAATGCAGAT 
TTTCGACGGG AGAATGTGCT GCCCCACTCT ACCTCCAGGT CCAGGCTGAC GTTACTGTTA 
CAGTCTCCCC CCGTGAAATT TCTCATAAGC CCAGAGTTCT TCACCGTGCT GCATTCTAAC 
CCTAGTGCCT ATCGCATGTT TACAAACAAT ACGTGTTTGA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CTCACCACTT TGAACGCTAC CAGCATAATC GAGACCTAGT GGGATTCCTC 
AACATGTTCG CTAACAAACA GCTGGAGCTG CCACGGGGCT GGGAAATGAA GCATGATCAC 
CAGGGCAAGG CTTTTTTTGT CGACCACAAT TCCCGTACCA CCACCTTCAT TGACCCTCGG 
CTCCCACTTC AGAGCAGCAG ACCAACAAGC GCTTTGGTAC ATCGGCAACA TCTGACCAGA 
CAGCGCAGTC ATAGTGCTGG TGAAGTAGGA GAGGACTCTC GACATTCAGG GCCACCAGTT 
CTTCCTAGGC CATCCAGCAC CTTCAATACA GTCAGTAGGT CACAGTACCA GGATATGGTT 
CCAGTAGCCT ACAATGACAA GATTGTTGCA TTTCTGCGTC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GGCAACCTGA TCTTACCAGG AACCACTCAC TCAGGGAGAA GATTCAGTTT 
ATCCGGACGG AGGGGACACC TGGACTGGTA CGTCTTTCCA GTGATGCTGA TCTTGTGATG 
TTGCTAAGTT TATTTGAAGA AGAGATAATG TCATATGTGC CTCCACATGC CTTACTCCAC 
CCCAGCTATT GCCAGTCCCC ACGCAGTTCC CCTGTGTCCT CGCCTCAGAA CTCTCCAGGT 
ACGCAGCGTG CCAATGCTCG AGCTCCCGCC CCTTATAAAC GAGATTTTGA AGCCAAACTA 
CGGAATTTCT ACAGGAAGTT GGAAACTAAA GGATATGGGC AAGGGCCCGG GAAGTTAAAG 
TTAATCATCA GAAGAGACCA TTTGTTGGAA GATGCTTTTA ATCAGATTAT GGGCTATTCC 
AGAAAAGACT TGCAGAGGAA TAAGCTCTAT GTCACCTTTG TTGGAGAGGA AGGGCTGGAT 
TACAGTGGAC CTTCTAGAGA GTTTTTCTTC CTGGTATCCA GAGAACTCTT TAATCCATAT 
TATGGCTTAT TTGAATATTC AGCTAATGAT ACATATACAG TACAAATAAG TCCCATGTCT 
GCTTTTGTAG ACAATCACCA CGAATGGTTC AGGTTTAGTG GTCGAATCCT TGGCCTTGCA 
CTGATACACC AGTATTTGTT AGATGCCTTC TTCACACGAC CATTTTATAA GGCACTCCTC 
AGAATTCTGT GTGATTTGAG TGATCTAGAA TACCTTGATG AGGAGTTCCA CCAGAGCCTT 
CAGTGGATGA AAGACAATGA CATACATGAT ATCCTAGACC TCACCTTTAC TGTAAATGAA 
GAAGTTTTTG GACAGATCAC TGAACGAGAA TTAAAGCCAG GGGGTGCCAA TATCCCAGTC 
ACAGAGAAGA ACAAGAAAGA ATACATCGAA AGAATGGTGA AATGGAGAAT TGAGAGGGGT 
GTTGTACAAC AAACAGAGAG CTTAGTACGA GGTTTTTATG AGGTAGTGGA TGCTAGACTG 
GTGTCTGTCT TTGATGCAAG AGAACTGGAA CTGGTCATTG CTGGAACTGC TGAAATAGAT 
TTAAGTGACT GGAGAAACAA CACAGAATAT AGAGGAGGAT ATCATGACAA TCATATTGTA 
ATCCGGTGGT TCTGGGCTGC TGTAGAAAGA TTTAACAATG AACAACGACT AAGGTTGTTA 
CAGTTTGTCA CAGGCACATC CAGCATACCC TATGAAGGCT TTGCTTCTCT GCGAGGGAGT 
AATGGGCCAA GGAGATTCTG TGTGGAGAAA TGGGGAAAAA TCACTGCCCT TCCCAGGGCT 
CACACTTGTT TTAATCGTCT GGACCTTCCC CCCTATCCAT CATTCTCCAT GCTTTATGAA 
AAACTTTTGA CAGCTGTAGA AGAAACAAGT ACTTTTGGAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031816151.1
CDS	625..4932
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHCRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTFAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGAHHDSPAVSNGPVSEESDGTPKHSFRTSSTMEIDPEDLTSSSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTSACAERSLGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLVMFSSASRTEEGNSASPAKPESSPAEGEEPPATEAASVENKQEEELAEVPDSSSVSDAAAERENGQEPQPSEEQGNGELGDSQEGDPPTSEADMGNLDLPGESRRAISETESIDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDAEGADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEEGACASEPAHGATAAGSQDSVCTPGSLPMVRVPSGQEEGQVADGPALADGEEMGEIWQRRNSLQATAESQAHEEDAGDAQNACEGAAAQAEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNERPEEHTNAIDGAGEDTDFHQSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRSQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRSSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_031960291.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031960291.1

ORF Insert Sequence:

ATGGAACCGG AAATAAAAAT ATGTTTTAAA TACTACCATG GCATTAGTGG AGCCTTGAGA 
GCTACTACCC CATGTATTAC TGTGAAAAAT CCTGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGTG CTTCAGGAAA TCAACATTGT CGGAAATTAG TCAGTTTTAC ATTGTCTGAT 
CTTAGGGCAG TTGGGCTAAA GAAAGGAATG TTCTTTAATC CTGATCCTTA CCTTAAGATG 
TCAATTCAGC CAGGAAAGAA GAGCAGTTTT CCTACATTTG CCCATCATGG GCAGGAACGA 
AGGTCTACCA TCATCAGTAA CACAACTAAT CCAATTTGGC ACAGAGAGAA ATACTCTTTT 
TTTGCACTTC TTACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGTCGT 
CCTATCATCA AGCGTTTTCT TGGGAAACTA ACCATACCAG TCCAGAGGTT GCTGGAGCGT 
CAAGCCATTG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT 
GTGAGTGGGT ATCTCCAATT CAAAGTTGAG ATTACATCTT CTATTCATGA AGATGCTTCC 
CCAGAAGCAG TTGGTACCAT CCTTGGAGTA AATTCTGTGA ACGGAGACCT GGGGAGTCCC 
TCTGATGATG AGGATATGCC GGGGGCACAT CACGACTCCC CTGCAGTTTC TAATGGTCCA 
GTTTCTGAGG AAAGTGATGG CACTCCCAAG CATTCCTTTA GGACTAGCTC TACTATGGAA 
ATAGACCCTG AGGACCTCAC TTCTAGTTCT TCCAGGACCT CCCCTCCCAG AGGACGGCAG 
GATTCGCTGA ACGATTATCT GGATGCCATT GAGCACAATG GTCATTCCAG GCCGGGGACA 
TCCGCCTGTG CTGAGAGGTC CCTGGGAGCT TCTCCCAAGC TGAGGAGCAG CTTCCCCACC 
GATACCAGAC TCAATGCCAT GCTTCACATT GACTCTGATG AGGAGGATCA TGAGTTTCAG 
CAAGATCTAG GTTATCCCTC TTCCTTGGAA GAGGAAGGGG GGCTAGTGAT GTTCAGTAGT 
GCTTCACGGA CTGAGGAGGG GAACTCGGCA AGTCCGGCAA AGCCAGAATC CAGCCCCGCT 
GAGGGGGAGG AACCCCCAGC AACTGAAGCC GCTTCTGTGG AGAATAAGCA GGAGGAGGAA 
CTTGCGGAGG TCCCTGACAG TTCCTCAGTT TCAGATGCAG CTGCCGAACG AGAAAATGGC 
CAGGAACCTC AGCCAAGTGA AGAGCAAGGC AATGGTGAAT TGGGTGACTC TCAAGAGGGT 
GACCCCCCTA CCAGCGAGGC AGACATGGGA AATTTGGATC TGCCTGGGGA AAGCAGAAGG 
GCCATTAGTG AAACTGAGTC GATTGACCAA GGGTCTGAGC CTTCCCAGGT ATCATCTGAG 
ACAGAACCCA GTGACCCCGC CCGGACCGAG AGCGTCAGCG AAGCCAGCAC CAGGCCCGAG 
GGGGAGAGCG ACGCCGAAGG GGCCGATAGT TCCTGCAACG AGAGTGTTAC CACGCAGCTG 
TCTTCGGTGG ATACCCGCTG CTCCTCTCTG GAGAGTGCCC GGTTTCCTGA AACTCCAGCT 
TTTTCGTCTC AGGAGGAAGA GGAGGGGGCT TGTGCTTCAG AACCTGCCCA TGGTGCCACT 
GCTGCAGGGT CTCAGGACTC CGTGTGCACC CCTGGGTCTT TGCCCATGGT AAGGGTACCC 
AGCGGTCAGG AGGAGGGACA GGTGGCAGAT GGGCCTGCGT TGGCTGATGG AGAGGAGATG 
GGCGAGATCT GGCAGAGGAG GAATAGTCTT CAGGCTACGG CAGAGAGCCA GGCCCACGAA 
GAAGATGCTG GGGATGCTCA AAACGCTTGT GAAGGGGCCG CAGCTCAAGC TGAAGGAGCT 
ACTGGAGGTT CTCAAGCCAA CGGCCACCAG CCATTGCGAT CGCTACCTTC AGTACGTCAG 
GATGTTAGCC GATACCAGAG GGTGGACGAG GCTCTGCCAC CAAATTGGGA GGCTCGGATT 
GACAGCCATG GAAGGATATT CTATGTGGAC CATGTAAACA GAACAACAAC GTGGCAGAGG 
CCCACTGCTC CCCCCGCCCC ACAGATACTT CAGAGATCCA ATTCTATACA ACAAATGGAA 
CAGCTAAACC GTAGGTATCA AAGTATCCGC AGGACAATGA CTAATGAAAG GCCTGAAGAA 
CACACTAATG CTATTGATGG AGCTGGAGAG GACACAGACT TCCATCAGTC CAATGCAGAT 
TTTCGACGGG AGAATGTGCT GCCCCACTCT ACCTCCAGGT CCAGGCTGAC GTTACTGTTA 
CAGTCTCCCC CCGTGAAATT TCTCATAAGC CCAGAGTTCT TCACCGTGCT GCATTCTAAC 
CCTAGTGCCT ATCGCATGTT TACAAACAAT ACGTGTTTGA AGCACATGAT CACCAAAGTC 
CGGCGGGACA CTCACCACTT TGAACGCTAC CAGCATAATC GAGACCTAGT GGGATTCCTC 
AACATGTTCG CTAACAAACA GCTGGAGCTG CCACGGGGCT GGGAAATGAA GCATGATCAC 
CAGGGCAAGG CTTTTTTTGT CGACCACAAT TCCCGTACCA CCACCTTCAT TGACCCTCGG 
CTCCCACTTC AGAGCAGCAG ACCAACAAGC GCTTTGGTAC ATCGGCAACA TCTGACCAGA 
CAGCGCAGTC ATAGTGCTGG TGAAGTAGGA GAGGACTCTC GACATTCAGG GCCACCAGTT 
CTTCCTAGGC CATCCAGCAC CTTCAATACA GTCAGTAGGT CACAGTACCA GGATATGGTT 
CCAGTAGCCT ACAATGACAA GATTGTTGCA TTTCTGCGTC AGCCCAATAT CTTTGAAATT 
CTACAGGAGC GGCAACCTGA TCTTACCAGG AACCACTCAC TCAGGGAGAA GATTCAGTTT 
ATCCGGACGG AGGGGACACC TGGACTGGTA CGTCTTTCCA GTGATGCTGA TCTTGTGATG 
TTGCTAAGTT TATTTGAAGA AGAGATAATG TCATATGTGC CTCCACATGC CTTACTCCAC 
CCCAGCTATT GCCAGTCCCC ACGCAGTTCC CCTGTGTCCT CGCCTCAGAA CTCTCCAGGT 
ACGCAGCGTG CCAATGCTCG AGCTCCCGCC CCTTATAAAC GAGATTTTGA AGCCAAACTA 
CGGAATTTCT ACAGGAAGTT GGAAACTAAA GGATATGGGC AAGGGCCCGG GAAGTTAAAG 
TTAATCATCA GAAGAGACCA TTTGTTGGAA GATGCTTTTA ATCAGATTAT GGGCTATTCC 
AGAAAAGACT TGCAGAGGAA TAAGCTCTAT GTCACCTTTG TTGGAGAGGA AGGGCTGGAT 
TACAGTGGAC CTTCTAGAGA GTTTTTCTTC CTGGTATCCA GAGAACTCTT TAATCCATAT 
TATGGCTTAT TTGAATATTC AGCTAATGAT ACATATACAG TACAAATAAG TCCCATGTCT 
GCTTTTGTAG ACAATCACCA CGAATGGTTC AGGTTTAGTG GTCGAATCCT TGGCCTTGCA 
CTGATACACC AGTATTTGTT AGATGCCTTC TTCACACGAC CATTTTATAA GGCACTCCTC 
AGAATTCTGT GTGATTTGAG TGATCTAGAA TACCTTGATG AGGAGTTCCA CCAGAGCCTT 
CAGTGGATGA AAGACAATGA CATACATGAT ATCCTAGACC TCACCTTTAC TGTAAATGAA 
GAAGTTTTTG GACAGATCAC TGAACGAGAA TTAAAGCCAG GGGGTGCCAA TATCCCAGTC 
ACAGAGAAGA ACAAGAAAGA ATACATCGAA AGAATGGTGA AATGGAGAAT TGAGAGGGGT 
GTTGTACAAC AAACAGAGAG CTTAGTACGA GGTTTTTATG AGGTAGTGGA TGCTAGACTG 
GTGTCTGTCT TTGATGCAAG AGAACTGGAA CTGGTCATTG CTGGAACTGC TGAAATAGAT 
TTAAGTGACT GGAGAAACAA CACAGAATAT AGAGGAGGAT ATCATGACAA TCATATTGTA 
ATCCGGTGGT TCTGGGCTGC TGTAGAAAGA TTTAACAATG AACAACGACT AAGGTTGTTA 
CAGTTTGTCA CAGGCACATC CAGCATACCC TATGAAGGCT TTGCTTCTCT GCGAGGGAGT 
AATGGGCCAA GGAGATTCTG TGTGGAGAAA TGGGGAAAAA TCACTGCCCT TCCCAGGGCT 
CACACTTGTT TTAATCGTCT GGACCTTCCC CCCTATCCAT CATTCTCCAT GCTTTATGAA 
AAACTTTTGA CAGCTGTAGA AGAAACAAGT ACTTTTGGAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_031960289.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031816149.1 Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OSa229931
Clone ID Related Accession (Same CDS sequence)	XM_031960289.1
Accession Version	XM_031960289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCAGAGA GCATCTGCTT TTTGTGAGGA GGCGCAACCC CCAGATGCGC 
TACACACTGA GTCCAGAGAA CCTTCAGAGT CTCGCCTCTC AGGGTTCCAT GCCCGAGAAC 
ATGAACCTTC AGAGGGCTAA TAGTGACACT GATCTGGTGA CTTCGGAAAG CCGCTCTAGC 
CTGACTGCAA GCATGTATGA ATATACCCTG GGCCAAGCCC AGAACCTCAT TATCTTCTGG 
GATATTAAAG AGGAAGTGGA CCCTACTGAC TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTTTGGGA TTCCAAAAAC CGAGGTGTTA CTGGGACACA AAAAGGACAA 
ATTGTTTGGA GAATTGAACC TGGGCCCTAT TTTATGGAAC CGGAAATAAA AATATGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCTTG AGAGCTACTA CCCCATGTAT TACTGTGAAA 
AATCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG AAATCAACAT 
TGTCGGAAAT TAGTCAGTTT TACATTGTCT GATCTTAGGG CAGTTGGGCT AAAGAAAGGA 
ATGTTCTTTA ATCCTGATCC TTACCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTTCCTACAT TTGCCCATCA TGGGCAGGAA CGAAGGTCTA CCATCATCAG TAACACAACT 
AATCCAATTT GGCACAGAGA GAAATACTCT TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCTATCA TCAAGCGTTT TCTTGGGAAA 
CTAACCATAC CAGTCCAGAG GTTGCTGGAG CGTCAAGCCA TTGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTATCTCCA ATTCAAAGTT 
GAGATTACAT CTTCTATTCA TGAAGATGCT TCCCCAGAAG CAGTTGGTAC CATCCTTGGA 
GTAAATTCTG TGAACGGAGA CCTGGGGAGT CCCTCTGATG ATGAGGATAT GCCGGGGGCA 
CATCACGACT CCCCTGCAGT TTCTAATGGT CCAGTTTCTG AGGAAAGTGA TGGCACTCCC 
AAGCATTCCT TTAGGACTAG CTCTACTATG GAAATAGACC CTGAGGACCT CACTTCTAGT 
TCTTCCAGGA CCTCCCCTCC CAGAGGACGG CAGGATTCGC TGAACGATTA TCTGGATGCC 
ATTGAGCACA ATGGTCATTC CAGGCCGGGG ACATCCGCCT GTGCTGAGAG GTCCCTGGGA 
GCTTCTCCCA AGCTGAGGAG CAGCTTCCCC ACCGATACCA GACTCAATGC CATGCTTCAC 
ATTGACTCTG ATGAGGAGGA TCATGAGTTT CAGCAAGATC TAGGTTATCC CTCTTCCTTG 
GAAGAGGAAG GGGGGCTAGT GATGTTCAGT AGTGCTTCAC GGACTGAGGA GGGGAACTCG 
GCAAGTCCGG CAAAGCCAGA ATCCAGCCCC GCTGAGGGGG AGGAACCCCC AGCAACTGAA 
GCCGCTTCTG TGGAGAATAA GCAGGAGGAG GAACTTGCGG AGGTCCCTGA CAGTTCCTCA 
GTTTCAGATG CAGCTGCCGA ACGAGAAAAT GGCCAGGAAC CTCAGCCAAG TGAAGAGCAA 
GGCAATGGTG AATTGGGTGA CTCTCAAGAG GGTGACCCCC CTACCAGCGA GGCAGACATG 
GGAAATTTGG ATCTGCCTGG GGAAAGCAGA AGGGCCATTA GTGAAACTGA GTCGATTGAC 
CAAGGGTCTG AGCCTTCCCA GGTATCATCT GAGACAGAAC CCAGTGACCC CGCCCGGACC 
GAGAGCGTCA GCGAAGCCAG CACCAGGCCC GAGGGGGAGA GCGACGCCGA AGGGGCCGAT 
AGTTCCTGCA ACGAGAGTGT TACCACGCAG CTGTCTTCGG TGGATACCCG CTGCTCCTCT 
CTGGAGAGTG CCCGGTTTCC TGAAACTCCA GCTTTTTCGT CTCAGGAGGA AGAGGAGGGG 
GCTTGTGCTT CAGAACCTGC CCATGGTGCC ACTGCTGCAG GGTCTCAGGA CTCCGTGTGC 
ACCCCTGGGT CTTTGCCCAT GGTAAGGGTA CCCAGCGGTC AGGAGGAGGG ACAGGTGGCA 
GATGGGCCTG CGTTGGCTGA TGGAGAGGAG ATGGGCGAGA TCTGGCAGAG GAGGAATAGT 
CTTCAGGCTA CGGCAGAGAG CCAGGCCCAC GAAGAAGATG CTGGGGATGC TCAAAACGCT 
TGTGAAGGGG CCGCAGCTCA AGCTGAAGGA GCTACTGGAG GTTCTCAAGC CAACGGCCAC 
CAGCCATTGC GATCGCTACC TTCAGTACGT CAGGATGTTA GCCGATACCA GAGGGTGGAC 
GAGGCTCTGC CACCAAATTG GGAGGCTCGG ATTGACAGCC ATGGAAGGAT ATTCTATGTG 
GACCATGTAA ACAGAACAAC AACGTGGCAG AGGCCCACTG CTCCCCCCGC CCCACAGATA 
CTTCAGAGAT CCAATTCTAT ACAACAAATG GAACAGCTAA ACCGTAGGTA TCAAAGTATC 
CGCAGGACAA TGACTAATGA AAGGCCTGAA GAACACACTA ATGCTATTGA TGGAGCTGGA 
GAGGACACAG ACTTCCATCA GTCCAATGCA GATTTTCGAC GGGAGAATGT GCTGCCCCAC 
TCTACCTCCA GGTCCAGGCT GACGTTACTG TTACAGTCTC CCCCCGTGAA ATTTCTCATA 
AGCCCAGAGT TCTTCACCGT GCTGCATTCT AACCCTAGTG CCTATCGCAT GTTTACAAAC 
AATACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACTCACCA CTTTGAACGC 
TACCAGCATA ATCGAGACCT AGTGGGATTC CTCAACATGT TCGCTAACAA ACAGCTGGAG 
CTGCCACGGG GCTGGGAAAT GAAGCATGAT CACCAGGGCA AGGCTTTTTT TGTCGACCAC 
AATTCCCGTA CCACCACCTT CATTGACCCT CGGCTCCCAC TTCAGAGCAG CAGACCAACA 
AGCGCTTTGG TACATCGGCA ACATCTGACC AGACAGCGCA GTCATAGTGC TGGTGAAGTA 
GGAGAGGACT CTCGACATTC AGGGCCACCA GTTCTTCCTA GGCCATCCAG CACCTTCAAT 
ACAGTCAGTA GGTCACAGTA CCAGGATATG GTTCCAGTAG CCTACAATGA CAAGATTGTT 
GCATTTCTGC GTCAGCCCAA TATCTTTGAA ATTCTACAGG AGCGGCAACC TGATCTTACC 
AGGAACCACT CACTCAGGGA GAAGATTCAG TTTATCCGGA CGGAGGGGAC ACCTGGACTG 
GTACGTCTTT CCAGTGATGC TGATCTTGTG ATGTTGCTAA GTTTATTTGA AGAAGAGATA 
ATGTCATATG TGCCTCCACA TGCCTTACTC CACCCCAGCT ATTGCCAGTC CCCACGCAGT 
TCCCCTGTGT CCTCGCCTCA GAACTCTCCA GGTACGCAGC GTGCCAATGC TCGAGCTCCC 
GCCCCTTATA AACGAGATTT TGAAGCCAAA CTACGGAATT TCTACAGGAA GTTGGAAACT 
AAAGGATATG GGCAAGGGCC CGGGAAGTTA AAGTTAATCA TCAGAAGAGA CCATTTGTTG 
GAAGATGCTT TTAATCAGAT TATGGGCTAT TCCAGAAAAG ACTTGCAGAG GAATAAGCTC 
TATGTCACCT TTGTTGGAGA GGAAGGGCTG GATTACAGTG GACCTTCTAG AGAGTTTTTC 
TTCCTGGTAT CCAGAGAACT CTTTAATCCA TATTATGGCT TATTTGAATA TTCAGCTAAT 
GATACATATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCACGAATGG 
TTCAGGTTTA GTGGTCGAAT CCTTGGCCTT GCACTGATAC ACCAGTATTT GTTAGATGCC 
TTCTTCACAC GACCATTTTA TAAGGCACTC CTCAGAATTC TGTGTGATTT GAGTGATCTA 
GAATACCTTG ATGAGGAGTT CCACCAGAGC CTTCAGTGGA TGAAAGACAA TGACATACAT 
GATATCCTAG ACCTCACCTT TACTGTAAAT GAAGAAGTTT TTGGACAGAT CACTGAACGA 
GAATTAAAGC CAGGGGGTGC CAATATCCCA GTCACAGAGA AGAACAAGAA AGAATACATC 
GAAAGAATGG TGAAATGGAG AATTGAGAGG GGTGTTGTAC AACAAACAGA GAGCTTAGTA 
CGAGGTTTTT ATGAGGTAGT GGATGCTAGA CTGGTGTCTG TCTTTGATGC AAGAGAACTG 
GAACTGGTCA TTGCTGGAAC TGCTGAAATA GATTTAAGTG ACTGGAGAAA CAACACAGAA 
TATAGAGGAG GATATCATGA CAATCATATT GTAATCCGGT GGTTCTGGGC TGCTGTAGAA 
AGATTTAACA ATGAACAACG ACTAAGGTTG TTACAGTTTG TCACAGGCAC ATCCAGCATA 
CCCTATGAAG GCTTTGCTTC TCTGCGAGGG AGTAATGGGC CAAGGAGATT CTGTGTGGAG 
AAATGGGGAA AAATCACTGC CCTTCCCAGG GCTCACACTT GTTTTAATCG TCTGGACCTT 
CCCCCCTATC CATCATTCTC CATGCTTTAT GAAAAACTTT TGACAGCTGT AGAAGAAACA 
AGTACTTTTG GACTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031816149.1
CDS	545..5245
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLASQGSMPENMNLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHCRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTFAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGAHHDSPAVSNGPVSEESDGTPKHSFRTSSTMEIDPEDLTSSSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTSACAERSLGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLVMFSSASRTEEGNSASPAKPESSPAEGEEPPATEAASVENKQEEELAEVPDSSSVSDAAAERENGQEPQPSEEQGNGELGDSQEGDPPTSEADMGNLDLPGESRRAISETESIDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDAEGADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEEGACASEPAHGATAAGSQDSVCTPGSLPMVRVPSGQEEGQVADGPALADGEEMGEIWQRRNSLQATAESQAHEEDAGDAQNACEGAAAQAEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNERPEEHTNAIDGAGEDTDFHQSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRSQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRSSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_031960289.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031960289.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCAGAGA GCATCTGCTT TTTGTGAGGA GGCGCAACCC CCAGATGCGC 
TACACACTGA GTCCAGAGAA CCTTCAGAGT CTCGCCTCTC AGGGTTCCAT GCCCGAGAAC 
ATGAACCTTC AGAGGGCTAA TAGTGACACT GATCTGGTGA CTTCGGAAAG CCGCTCTAGC 
CTGACTGCAA GCATGTATGA ATATACCCTG GGCCAAGCCC AGAACCTCAT TATCTTCTGG 
GATATTAAAG AGGAAGTGGA CCCTACTGAC TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTTTGGGA TTCCAAAAAC CGAGGTGTTA CTGGGACACA AAAAGGACAA 
ATTGTTTGGA GAATTGAACC TGGGCCCTAT TTTATGGAAC CGGAAATAAA AATATGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCTTG AGAGCTACTA CCCCATGTAT TACTGTGAAA 
AATCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG AAATCAACAT 
TGTCGGAAAT TAGTCAGTTT TACATTGTCT GATCTTAGGG CAGTTGGGCT AAAGAAAGGA 
ATGTTCTTTA ATCCTGATCC TTACCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTTCCTACAT TTGCCCATCA TGGGCAGGAA CGAAGGTCTA CCATCATCAG TAACACAACT 
AATCCAATTT GGCACAGAGA GAAATACTCT TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCTATCA TCAAGCGTTT TCTTGGGAAA 
CTAACCATAC CAGTCCAGAG GTTGCTGGAG CGTCAAGCCA TTGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTATCTCCA ATTCAAAGTT 
GAGATTACAT CTTCTATTCA TGAAGATGCT TCCCCAGAAG CAGTTGGTAC CATCCTTGGA 
GTAAATTCTG TGAACGGAGA CCTGGGGAGT CCCTCTGATG ATGAGGATAT GCCGGGGGCA 
CATCACGACT CCCCTGCAGT TTCTAATGGT CCAGTTTCTG AGGAAAGTGA TGGCACTCCC 
AAGCATTCCT TTAGGACTAG CTCTACTATG GAAATAGACC CTGAGGACCT CACTTCTAGT 
TCTTCCAGGA CCTCCCCTCC CAGAGGACGG CAGGATTCGC TGAACGATTA TCTGGATGCC 
ATTGAGCACA ATGGTCATTC CAGGCCGGGG ACATCCGCCT GTGCTGAGAG GTCCCTGGGA 
GCTTCTCCCA AGCTGAGGAG CAGCTTCCCC ACCGATACCA GACTCAATGC CATGCTTCAC 
ATTGACTCTG ATGAGGAGGA TCATGAGTTT CAGCAAGATC TAGGTTATCC CTCTTCCTTG 
GAAGAGGAAG GGGGGCTAGT GATGTTCAGT AGTGCTTCAC GGACTGAGGA GGGGAACTCG 
GCAAGTCCGG CAAAGCCAGA ATCCAGCCCC GCTGAGGGGG AGGAACCCCC AGCAACTGAA 
GCCGCTTCTG TGGAGAATAA GCAGGAGGAG GAACTTGCGG AGGTCCCTGA CAGTTCCTCA 
GTTTCAGATG CAGCTGCCGA ACGAGAAAAT GGCCAGGAAC CTCAGCCAAG TGAAGAGCAA 
GGCAATGGTG AATTGGGTGA CTCTCAAGAG GGTGACCCCC CTACCAGCGA GGCAGACATG 
GGAAATTTGG ATCTGCCTGG GGAAAGCAGA AGGGCCATTA GTGAAACTGA GTCGATTGAC 
CAAGGGTCTG AGCCTTCCCA GGTATCATCT GAGACAGAAC CCAGTGACCC CGCCCGGACC 
GAGAGCGTCA GCGAAGCCAG CACCAGGCCC GAGGGGGAGA GCGACGCCGA AGGGGCCGAT 
AGTTCCTGCA ACGAGAGTGT TACCACGCAG CTGTCTTCGG TGGATACCCG CTGCTCCTCT 
CTGGAGAGTG CCCGGTTTCC TGAAACTCCA GCTTTTTCGT CTCAGGAGGA AGAGGAGGGG 
GCTTGTGCTT CAGAACCTGC CCATGGTGCC ACTGCTGCAG GGTCTCAGGA CTCCGTGTGC 
ACCCCTGGGT CTTTGCCCAT GGTAAGGGTA CCCAGCGGTC AGGAGGAGGG ACAGGTGGCA 
GATGGGCCTG CGTTGGCTGA TGGAGAGGAG ATGGGCGAGA TCTGGCAGAG GAGGAATAGT 
CTTCAGGCTA CGGCAGAGAG CCAGGCCCAC GAAGAAGATG CTGGGGATGC TCAAAACGCT 
TGTGAAGGGG CCGCAGCTCA AGCTGAAGGA GCTACTGGAG GTTCTCAAGC CAACGGCCAC 
CAGCCATTGC GATCGCTACC TTCAGTACGT CAGGATGTTA GCCGATACCA GAGGGTGGAC 
GAGGCTCTGC CACCAAATTG GGAGGCTCGG ATTGACAGCC ATGGAAGGAT ATTCTATGTG 
GACCATGTAA ACAGAACAAC AACGTGGCAG AGGCCCACTG CTCCCCCCGC CCCACAGATA 
CTTCAGAGAT CCAATTCTAT ACAACAAATG GAACAGCTAA ACCGTAGGTA TCAAAGTATC 
CGCAGGACAA TGACTAATGA AAGGCCTGAA GAACACACTA ATGCTATTGA TGGAGCTGGA 
GAGGACACAG ACTTCCATCA GTCCAATGCA GATTTTCGAC GGGAGAATGT GCTGCCCCAC 
TCTACCTCCA GGTCCAGGCT GACGTTACTG TTACAGTCTC CCCCCGTGAA ATTTCTCATA 
AGCCCAGAGT TCTTCACCGT GCTGCATTCT AACCCTAGTG CCTATCGCAT GTTTACAAAC 
AATACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACTCACCA CTTTGAACGC 
TACCAGCATA ATCGAGACCT AGTGGGATTC CTCAACATGT TCGCTAACAA ACAGCTGGAG 
CTGCCACGGG GCTGGGAAAT GAAGCATGAT CACCAGGGCA AGGCTTTTTT TGTCGACCAC 
AATTCCCGTA CCACCACCTT CATTGACCCT CGGCTCCCAC TTCAGAGCAG CAGACCAACA 
AGCGCTTTGG TACATCGGCA ACATCTGACC AGACAGCGCA GTCATAGTGC TGGTGAAGTA 
GGAGAGGACT CTCGACATTC AGGGCCACCA GTTCTTCCTA GGCCATCCAG CACCTTCAAT 
ACAGTCAGTA GGTCACAGTA CCAGGATATG GTTCCAGTAG CCTACAATGA CAAGATTGTT 
GCATTTCTGC GTCAGCCCAA TATCTTTGAA ATTCTACAGG AGCGGCAACC TGATCTTACC 
AGGAACCACT CACTCAGGGA GAAGATTCAG TTTATCCGGA CGGAGGGGAC ACCTGGACTG 
GTACGTCTTT CCAGTGATGC TGATCTTGTG ATGTTGCTAA GTTTATTTGA AGAAGAGATA 
ATGTCATATG TGCCTCCACA TGCCTTACTC CACCCCAGCT ATTGCCAGTC CCCACGCAGT 
TCCCCTGTGT CCTCGCCTCA GAACTCTCCA GGTACGCAGC GTGCCAATGC TCGAGCTCCC 
GCCCCTTATA AACGAGATTT TGAAGCCAAA CTACGGAATT TCTACAGGAA GTTGGAAACT 
AAAGGATATG GGCAAGGGCC CGGGAAGTTA AAGTTAATCA TCAGAAGAGA CCATTTGTTG 
GAAGATGCTT TTAATCAGAT TATGGGCTAT TCCAGAAAAG ACTTGCAGAG GAATAAGCTC 
TATGTCACCT TTGTTGGAGA GGAAGGGCTG GATTACAGTG GACCTTCTAG AGAGTTTTTC 
TTCCTGGTAT CCAGAGAACT CTTTAATCCA TATTATGGCT TATTTGAATA TTCAGCTAAT 
GATACATATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCACGAATGG 
TTCAGGTTTA GTGGTCGAAT CCTTGGCCTT GCACTGATAC ACCAGTATTT GTTAGATGCC 
TTCTTCACAC GACCATTTTA TAAGGCACTC CTCAGAATTC TGTGTGATTT GAGTGATCTA 
GAATACCTTG ATGAGGAGTT CCACCAGAGC CTTCAGTGGA TGAAAGACAA TGACATACAT 
GATATCCTAG ACCTCACCTT TACTGTAAAT GAAGAAGTTT TTGGACAGAT CACTGAACGA 
GAATTAAAGC CAGGGGGTGC CAATATCCCA GTCACAGAGA AGAACAAGAA AGAATACATC 
GAAAGAATGG TGAAATGGAG AATTGAGAGG GGTGTTGTAC AACAAACAGA GAGCTTAGTA 
CGAGGTTTTT ATGAGGTAGT GGATGCTAGA CTGGTGTCTG TCTTTGATGC AAGAGAACTG 
GAACTGGTCA TTGCTGGAAC TGCTGAAATA GATTTAAGTG ACTGGAGAAA CAACACAGAA 
TATAGAGGAG GATATCATGA CAATCATATT GTAATCCGGT GGTTCTGGGC TGCTGTAGAA 
AGATTTAACA ATGAACAACG ACTAAGGTTG TTACAGTTTG TCACAGGCAC ATCCAGCATA 
CCCTATGAAG GCTTTGCTTC TCTGCGAGGG AGTAATGGGC CAAGGAGATT CTGTGTGGAG 
AAATGGGGAA AAATCACTGC CCTTCCCAGG GCTCACACTT GTTTTAATCG TCTGGACCTT 
CCCCCCTATC CATCATTCTC CATGCTTTAT GAAAAACTTT TGACAGCTGT AGAAGAAACA 
AGTACTTTTG GACTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_031960290.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031816150.1 Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

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CloneID	OSa229932
Clone ID Related Accession (Same CDS sequence)	XM_031960290.1
Accession Version	XM_031960290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCAGAGA GCATCTGCTT TTTGTGAGGA GGCGCAACCC CCAGATGCGC 
TACACACTGA GTCCAGAGAA CCTTCAGAGT CTCGCCTCTC AGGGTTCCAT GCCCGAGAAC 
ATGAACCTTC AGAGGGCTAA TAGTGACACT GATCTGGTGA CTTCGGAAAG CCGCTCTAGC 
CTGACTGCAA GCATGTATGA ATATACCCTG GGCCAAGCCC AGAACCTCAT TATCTTCTGG 
GATATTAAAG AGGAAGTGGA CCCTACTGAC TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTTTGGGA TTCCAAAAAC CGAGGTGTTA CTGGGACACA AAAAGGACAA 
ATTGTTTGGA GAATTGAACC TGGGCCCTAT TTTATGGAAC CGGAAATAAA AATATGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCTTG AGAGCTACTA CCCCATGTAT TACTGTGAAA 
AATCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG AAATCAACAT 
TGTCGGAAAT TAGTCAGTTT TACATTGTCT GATCTTAGGG CAGTTGGGCT AAAGAAAGGA 
ATGTTCTTTA ATCCTGATCC TTACCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTTCCTACAT TTGCCCATCA TGGGCAGGAA CGAAGGTCTA CCATCATCAG TAACACAACT 
AATCCAATTT GGCACAGAGA GAAATACTCT TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCTATCA TCAAGCGTTT TCTTGGGAAA 
CTAACCATAC CAGTCCAGAG GTTGCTGGAG CGTCAAGCCA TTGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTATCTCCA ATTCAAAGTT 
GAGATTACAT CTTCTATTCA TGAAGATGCT TCCCCAGAAG CAGTTGGTAC CATCCTTGGA 
GTAAATTCTG TGAACGGAGA CCTGGGGAGT CCCTCTGATG ATGAGGATAT GCCGGGGGCA 
CATCACGACT CCCCTGCAGT TTCTAATGGT CCAGTTTCTG AGGAAAGTGA TGGCACTCCC 
AAGCATTCCT TTAGGACTAG CTCTACTATG GAAATAGACC CTGAGGACCT CACTTCTAGT 
TCTTCCAGGA CCTCCCCTCC CAGAGGACGG CAGGATTCGC TGAACGATTA TCTGGATGCC 
ATTGAGCACA ATGGTCATTC CAGGCCGGGG ACATCCGCCT GTGCTGAGAG GTCCCTGGGA 
GCTTCTCCCA AGCTGAGGAG CAGCTTCCCC ACCGATACCA GACTCAATGC CATGCTTCAC 
ATTGACTCTG ATGAGGAGGA TCATGAGTTT CAGCAAGATC TAGGTTATCC CTCTTCCTTG 
GAAGAGGAAG GGGGGCTAGT GATGTTCAGT AGTGCTTCAC GGACTGAGGA GGGGAACTCG 
GCAAGTCCGG CAAAGCCAGA ATCCAGCCCC GCTGAGGGGG AGGAACCCCC AGCAACTGAA 
GCCGCTTCTG TGGAGAATAA GCAGGAGGAG GAACTTGCGG AGGTCCCTGA CAGTTCCTCA 
GTTTCAGATG CAGCTGCCGA ACGAGAAAAT GGCCAGGAAC CTCAGCCAAG TGAAGAGCAA 
GGCAATGGTG AATTGGGTGA CTCTCAAGAG GGTGACCCCC CTACCAGCGA GGCAGACATG 
GGAAATTTGG ATCTGCCTGG GGAAAGCAGA AGGGCCATTA GTGAAACTGA GTCGATTGAC 
CAAGGGTCTG AGCCTTCCCA GGTATCATCT GAGACAGAAC CCAGTGACCC CGCCCGGACC 
GAGAGCGTCA GCGAAGCCAG CACCAGGCCC GAGGGGGAGA GCGACGCCGA AGGGGCCGAT 
AGTTCCTGCA ACGAGAGTGT TACCACGCAG CTGTCTTCGG TGGATACCCG CTGCTCCTCT 
CTGGAGAGTG CCCGGTTTCC TGAAACTCCA GCTTTTTCGT CTCAGGAGGA AGAGGAGGGG 
GCTTGTGCTT CAGAACCTGC CCATGGTGCC ACTGCTGCAG GGTCTCAGGA CTCCGTGTGC 
ACCCCTGGGT CTTTGCCCAT GGTAAGGGTA CCCAGCGGTC AGGAGGAGGG ACAGGTGGCA 
GATGGGCCTG CGTTGGCTGA TGGAGAGGAG ATGGGCGAGA TCTGGCAGAG GAGGAATAGT 
CTTCAGGCTA CGGCAGAGAG CCAGGCCCAC GAAGAAGATG CTGGGGATGC TCAAAACGCT 
TGTGAAGGGG CCGCAGCTCA AGCTGAAGGA GCTACTGGAG ATTGGGAGGC TCGGATTGAC 
AGCCATGGAA GGATATTCTA TGTGGACCAT GTAAACAGAA CAACAACGTG GCAGAGGCCC 
ACTGCTCCCC CCGCCCCACA GATACTTCAG AGATCCAATT CTATACAACA AATGGAACAG 
CTAAACCGTA GGTATCAAAG TATCCGCAGG ACAATGACTA ATGAAAGGCC TGAAGAACAC 
ACTAATGCTA TTGATGGAGC TGGAGAGGAC ACAGACTTCC ATCAGTCCAA TGCAGATTTT 
CGACGGGAGA ATGTGCTGCC CCACTCTACC TCCAGGTCCA GGCTGACGTT ACTGTTACAG 
TCTCCCCCCG TGAAATTTCT CATAAGCCCA GAGTTCTTCA CCGTGCTGCA TTCTAACCCT 
AGTGCCTATC GCATGTTTAC AAACAATACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACTC ACCACTTTGA ACGCTACCAG CATAATCGAG ACCTAGTGGG ATTCCTCAAC 
ATGTTCGCTA ACAAACAGCT GGAGCTGCCA CGGGGCTGGG AAATGAAGCA TGATCACCAG 
GGCAAGGCTT TTTTTGTCGA CCACAATTCC CGTACCACCA CCTTCATTGA CCCTCGGCTC 
CCACTTCAGA GCAGCAGACC AACAAGCGCT TTGGTACATC GGCAACATCT GACCAGACAG 
CGCAGTCATA GTGCTGGTGA AGTAGGAGAG GACTCTCGAC ATTCAGGGCC ACCAGTTCTT 
CCTAGGCCAT CCAGCACCTT CAATACAGTC AGTAGGTCAC AGTACCAGGA TATGGTTCCA 
GTAGCCTACA ATGACAAGAT TGTTGCATTT CTGCGTCAGC CCAATATCTT TGAAATTCTA 
CAGGAGCGGC AACCTGATCT TACCAGGAAC CACTCACTCA GGGAGAAGAT TCAGTTTATC 
CGGACGGAGG GGACACCTGG ACTGGTACGT CTTTCCAGTG ATGCTGATCT TGTGATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TATGTGCCTC CACATGCCTT ACTCCACCCC 
AGCTATTGCC AGTCCCCACG CAGTTCCCCT GTGTCCTCGC CTCAGAACTC TCCAGGTACG 
CAGCGTGCCA ATGCTCGAGC TCCCGCCCCT TATAAACGAG ATTTTGAAGC CAAACTACGG 
AATTTCTACA GGAAGTTGGA AACTAAAGGA TATGGGCAAG GGCCCGGGAA GTTAAAGTTA 
ATCATCAGAA GAGACCATTT GTTGGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACTTGC AGAGGAATAA GCTCTATGTC ACCTTTGTTG GAGAGGAAGG GCTGGATTAC 
AGTGGACCTT CTAGAGAGTT TTTCTTCCTG GTATCCAGAG AACTCTTTAA TCCATATTAT 
GGCTTATTTG AATATTCAGC TAATGATACA TATACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCACGA ATGGTTCAGG TTTAGTGGTC GAATCCTTGG CCTTGCACTG 
ATACACCAGT ATTTGTTAGA TGCCTTCTTC ACACGACCAT TTTATAAGGC ACTCCTCAGA 
ATTCTGTGTG ATTTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTTCAG 
TGGATGAAAG ACAATGACAT ACATGATATC CTAGACCTCA CCTTTACTGT AAATGAAGAA 
GTTTTTGGAC AGATCACTGA ACGAGAATTA AAGCCAGGGG GTGCCAATAT CCCAGTCACA 
GAGAAGAACA AGAAAGAATA CATCGAAAGA ATGGTGAAAT GGAGAATTGA GAGGGGTGTT 
GTACAACAAA CAGAGAGCTT AGTACGAGGT TTTTATGAGG TAGTGGATGC TAGACTGGTG 
TCTGTCTTTG ATGCAAGAGA ACTGGAACTG GTCATTGCTG GAACTGCTGA AATAGATTTA 
AGTGACTGGA GAAACAACAC AGAATATAGA GGAGGATATC ATGACAATCA TATTGTAATC 
CGGTGGTTCT GGGCTGCTGT AGAAAGATTT AACAATGAAC AACGACTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATACCCTAT GAAGGCTTTG CTTCTCTGCG AGGGAGTAAT 
GGGCCAAGGA GATTCTGTGT GGAGAAATGG GGAAAAATCA CTGCCCTTCC CAGGGCTCAC 
ACTTGTTTTA ATCGTCTGGA CCTTCCCCCC TATCCATCAT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CTGTAGAAGA AACAAGTACT TTTGGACTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031816150.1
CDS	546..5150
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLASQGSMPENMNLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHCRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTFAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEITSSIHEDASPEAVGTILGVNSVNGDLGSPSDDEDMPGAHHDSPAVSNGPVSEESDGTPKHSFRTSSTMEIDPEDLTSSSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTSACAERSLGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLVMFSSASRTEEGNSASPAKPESSPAEGEEPPATEAASVENKQEEELAEVPDSSSVSDAAAERENGQEPQPSEEQGNGELGDSQEGDPPTSEADMGNLDLPGESRRAISETESIDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDAEGADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEEGACASEPAHGATAAGSQDSVCTPGSLPMVRVPSGQEEGQVADGPALADGEEMGEIWQRRNSLQATAESQAHEEDAGDAQNACEGAAAQAEGATGDWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNERPEEHTNAIDGAGEDTDFHQSNADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRSQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRSSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_031960290.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031960290.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCAGAGA GCATCTGCTT TTTGTGAGGA GGCGCAACCC CCAGATGCGC 
TACACACTGA GTCCAGAGAA CCTTCAGAGT CTCGCCTCTC AGGGTTCCAT GCCCGAGAAC 
ATGAACCTTC AGAGGGCTAA TAGTGACACT GATCTGGTGA CTTCGGAAAG CCGCTCTAGC 
CTGACTGCAA GCATGTATGA ATATACCCTG GGCCAAGCCC AGAACCTCAT TATCTTCTGG 
GATATTAAAG AGGAAGTGGA CCCTACTGAC TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTTTGGGA TTCCAAAAAC CGAGGTGTTA CTGGGACACA AAAAGGACAA 
ATTGTTTGGA GAATTGAACC TGGGCCCTAT TTTATGGAAC CGGAAATAAA AATATGTTTT 
AAATACTACC ATGGCATTAG TGGAGCCTTG AGAGCTACTA CCCCATGTAT TACTGTGAAA 
AATCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG AAATCAACAT 
TGTCGGAAAT TAGTCAGTTT TACATTGTCT GATCTTAGGG CAGTTGGGCT AAAGAAAGGA 
ATGTTCTTTA ATCCTGATCC TTACCTTAAG ATGTCAATTC AGCCAGGAAA GAAGAGCAGT 
TTTCCTACAT TTGCCCATCA TGGGCAGGAA CGAAGGTCTA CCATCATCAG TAACACAACT 
AATCCAATTT GGCACAGAGA GAAATACTCT TTTTTTGCAC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCTATCA TCAAGCGTTT TCTTGGGAAA 
CTAACCATAC CAGTCCAGAG GTTGCTGGAG CGTCAAGCCA TTGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTATCTCCA ATTCAAAGTT 
GAGATTACAT CTTCTATTCA TGAAGATGCT TCCCCAGAAG CAGTTGGTAC CATCCTTGGA 
GTAAATTCTG TGAACGGAGA CCTGGGGAGT CCCTCTGATG ATGAGGATAT GCCGGGGGCA 
CATCACGACT CCCCTGCAGT TTCTAATGGT CCAGTTTCTG AGGAAAGTGA TGGCACTCCC 
AAGCATTCCT TTAGGACTAG CTCTACTATG GAAATAGACC CTGAGGACCT CACTTCTAGT 
TCTTCCAGGA CCTCCCCTCC CAGAGGACGG CAGGATTCGC TGAACGATTA TCTGGATGCC 
ATTGAGCACA ATGGTCATTC CAGGCCGGGG ACATCCGCCT GTGCTGAGAG GTCCCTGGGA 
GCTTCTCCCA AGCTGAGGAG CAGCTTCCCC ACCGATACCA GACTCAATGC CATGCTTCAC 
ATTGACTCTG ATGAGGAGGA TCATGAGTTT CAGCAAGATC TAGGTTATCC CTCTTCCTTG 
GAAGAGGAAG GGGGGCTAGT GATGTTCAGT AGTGCTTCAC GGACTGAGGA GGGGAACTCG 
GCAAGTCCGG CAAAGCCAGA ATCCAGCCCC GCTGAGGGGG AGGAACCCCC AGCAACTGAA 
GCCGCTTCTG TGGAGAATAA GCAGGAGGAG GAACTTGCGG AGGTCCCTGA CAGTTCCTCA 
GTTTCAGATG CAGCTGCCGA ACGAGAAAAT GGCCAGGAAC CTCAGCCAAG TGAAGAGCAA 
GGCAATGGTG AATTGGGTGA CTCTCAAGAG GGTGACCCCC CTACCAGCGA GGCAGACATG 
GGAAATTTGG ATCTGCCTGG GGAAAGCAGA AGGGCCATTA GTGAAACTGA GTCGATTGAC 
CAAGGGTCTG AGCCTTCCCA GGTATCATCT GAGACAGAAC CCAGTGACCC CGCCCGGACC 
GAGAGCGTCA GCGAAGCCAG CACCAGGCCC GAGGGGGAGA GCGACGCCGA AGGGGCCGAT 
AGTTCCTGCA ACGAGAGTGT TACCACGCAG CTGTCTTCGG TGGATACCCG CTGCTCCTCT 
CTGGAGAGTG CCCGGTTTCC TGAAACTCCA GCTTTTTCGT CTCAGGAGGA AGAGGAGGGG 
GCTTGTGCTT CAGAACCTGC CCATGGTGCC ACTGCTGCAG GGTCTCAGGA CTCCGTGTGC 
ACCCCTGGGT CTTTGCCCAT GGTAAGGGTA CCCAGCGGTC AGGAGGAGGG ACAGGTGGCA 
GATGGGCCTG CGTTGGCTGA TGGAGAGGAG ATGGGCGAGA TCTGGCAGAG GAGGAATAGT 
CTTCAGGCTA CGGCAGAGAG CCAGGCCCAC GAAGAAGATG CTGGGGATGC TCAAAACGCT 
TGTGAAGGGG CCGCAGCTCA AGCTGAAGGA GCTACTGGAG ATTGGGAGGC TCGGATTGAC 
AGCCATGGAA GGATATTCTA TGTGGACCAT GTAAACAGAA CAACAACGTG GCAGAGGCCC 
ACTGCTCCCC CCGCCCCACA GATACTTCAG AGATCCAATT CTATACAACA AATGGAACAG 
CTAAACCGTA GGTATCAAAG TATCCGCAGG ACAATGACTA ATGAAAGGCC TGAAGAACAC 
ACTAATGCTA TTGATGGAGC TGGAGAGGAC ACAGACTTCC ATCAGTCCAA TGCAGATTTT 
CGACGGGAGA ATGTGCTGCC CCACTCTACC TCCAGGTCCA GGCTGACGTT ACTGTTACAG 
TCTCCCCCCG TGAAATTTCT CATAAGCCCA GAGTTCTTCA CCGTGCTGCA TTCTAACCCT 
AGTGCCTATC GCATGTTTAC AAACAATACG TGTTTGAAGC ACATGATCAC CAAAGTCCGG 
CGGGACACTC ACCACTTTGA ACGCTACCAG CATAATCGAG ACCTAGTGGG ATTCCTCAAC 
ATGTTCGCTA ACAAACAGCT GGAGCTGCCA CGGGGCTGGG AAATGAAGCA TGATCACCAG 
GGCAAGGCTT TTTTTGTCGA CCACAATTCC CGTACCACCA CCTTCATTGA CCCTCGGCTC 
CCACTTCAGA GCAGCAGACC AACAAGCGCT TTGGTACATC GGCAACATCT GACCAGACAG 
CGCAGTCATA GTGCTGGTGA AGTAGGAGAG GACTCTCGAC ATTCAGGGCC ACCAGTTCTT 
CCTAGGCCAT CCAGCACCTT CAATACAGTC AGTAGGTCAC AGTACCAGGA TATGGTTCCA 
GTAGCCTACA ATGACAAGAT TGTTGCATTT CTGCGTCAGC CCAATATCTT TGAAATTCTA 
CAGGAGCGGC AACCTGATCT TACCAGGAAC CACTCACTCA GGGAGAAGAT TCAGTTTATC 
CGGACGGAGG GGACACCTGG ACTGGTACGT CTTTCCAGTG ATGCTGATCT TGTGATGTTG 
CTAAGTTTAT TTGAAGAAGA GATAATGTCA TATGTGCCTC CACATGCCTT ACTCCACCCC 
AGCTATTGCC AGTCCCCACG CAGTTCCCCT GTGTCCTCGC CTCAGAACTC TCCAGGTACG 
CAGCGTGCCA ATGCTCGAGC TCCCGCCCCT TATAAACGAG ATTTTGAAGC CAAACTACGG 
AATTTCTACA GGAAGTTGGA AACTAAAGGA TATGGGCAAG GGCCCGGGAA GTTAAAGTTA 
ATCATCAGAA GAGACCATTT GTTGGAAGAT GCTTTTAATC AGATTATGGG CTATTCCAGA 
AAAGACTTGC AGAGGAATAA GCTCTATGTC ACCTTTGTTG GAGAGGAAGG GCTGGATTAC 
AGTGGACCTT CTAGAGAGTT TTTCTTCCTG GTATCCAGAG AACTCTTTAA TCCATATTAT 
GGCTTATTTG AATATTCAGC TAATGATACA TATACAGTAC AAATAAGTCC CATGTCTGCT 
TTTGTAGACA ATCACCACGA ATGGTTCAGG TTTAGTGGTC GAATCCTTGG CCTTGCACTG 
ATACACCAGT ATTTGTTAGA TGCCTTCTTC ACACGACCAT TTTATAAGGC ACTCCTCAGA 
ATTCTGTGTG ATTTGAGTGA TCTAGAATAC CTTGATGAGG AGTTCCACCA GAGCCTTCAG 
TGGATGAAAG ACAATGACAT ACATGATATC CTAGACCTCA CCTTTACTGT AAATGAAGAA 
GTTTTTGGAC AGATCACTGA ACGAGAATTA AAGCCAGGGG GTGCCAATAT CCCAGTCACA 
GAGAAGAACA AGAAAGAATA CATCGAAAGA ATGGTGAAAT GGAGAATTGA GAGGGGTGTT 
GTACAACAAA CAGAGAGCTT AGTACGAGGT TTTTATGAGG TAGTGGATGC TAGACTGGTG 
TCTGTCTTTG ATGCAAGAGA ACTGGAACTG GTCATTGCTG GAACTGCTGA AATAGATTTA 
AGTGACTGGA GAAACAACAC AGAATATAGA GGAGGATATC ATGACAATCA TATTGTAATC 
CGGTGGTTCT GGGCTGCTGT AGAAAGATTT AACAATGAAC AACGACTAAG GTTGTTACAG 
TTTGTCACAG GCACATCCAG CATACCCTAT GAAGGCTTTG CTTCTCTGCG AGGGAGTAAT 
GGGCCAAGGA GATTCTGTGT GGAGAAATGG GGAAAAATCA CTGCCCTTCC CAGGGCTCAC 
ACTTGTTTTA ATCGTCTGGA CCTTCCCCCC TATCCATCAT TCTCCATGCT TTATGAAAAA 
CTTTTGACAG CTGTAGAAGA AACAAGTACT TTTGGACTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECW2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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