HEATR1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEATR1
Entrez Gene ID	100917590
Full Name	HEAT repeat containing 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012547958.3	XP_012403412.1	HEAT repeat-containing protein 1 isoform X2
XM_023502057.2	XP_023357825.1	HEAT repeat-containing protein 1 isoform X1
XM_023502057.1	XP_023357825.1	HEAT repeat-containing protein 1 isoform X1
XM_012547958.1	XP_012403412.1	HEAT repeat-containing protein 1
XM_012547958.2	XP_012403412.1	HEAT repeat-containing protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following HEATR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEATR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa129748	XM_012547958.3 Latest version!	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa154067	XM_023502057.2 Latest version!	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OSa154067	XM_023502057.1	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa129748	XM_012547958.1	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa129748	XM_012547958.2	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEATR1 ( XM_012547958.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012403412.1 Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa129748
Clone ID Related Accession (Same CDS sequence)	XM_012547958.3 , XM_012547958.1 , XM_012547958.2
Accession Version	XM_012547958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012547958.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAAC TGAGGAACTG GACAGTCATA TATATGCTAG ACTCTTAGAA 
GATATACTTA AAGATATATC ATTGGAAAAC AACTTAGACC ACTTGTTGGC TAGCACTCTT 
CTTGAAGAAT ATATTTCATA TAGTTCAAGG AAAGAAATAG ATTCTAAGGA GATATCACTA 
CTCAATGAAC AACTCCTTCC TCTCATTAGA CTTTTGGAAA GCAAATACCC TAAGACATTA 
GATTTGGTAT TGGAAGACCA TCTGAAGGAT GTTACAGATC CCCAAAAGCA AGAATTTTTT 
CATCAATTTA TTTCTCTCTC CACAAGTGGA GGAAAGTTTC AGTTTTTAGC AGATTCAGAT 
TCTTGCCTCA CGCTGAGCTT GAACCATCCA CTGGCTCCAG TGAGACTTTT GGCAGTTAAC 
CATCTGAAAA ATATTATGGA AACATCAAAG GACAATATTG ATGCTTCTTT CATAAAAGAA 
GCTATTATAA TGCGTCTTGG TGATGATAAT TTAGATGTTG TTCTGTCAGC CATGAATGCT 
TTTGTGGTTT TCAAAAAAGA GTTTAGCCCA GAAGAAACAG TTTCAAGTCT TTTGAGACTT 
TTTCAAAGAG CAGAACTTTC ACAGGATGGG GCATGGTACA AAATACTTGA ATTAGCAGCT 
GACACTCTAA TTAAAGAAGA GATTCTGAAT GGAAATGATG AGTTACTAAA TCAAGTGGTC 
ATACATTTGC TGCCATTTAT GGTTGTTACC AATGACAATT CAAAATCCCA AGAAATAAAA 
ATGGCTACTT ATCTGTCAAA ATCAGGAATT TGCTCTTTGC ACCCTATACT GAAAGGATGG 
AAAGAAGCTC TTGAAAAAGT GATTAAAACC ACAAAGCCAA CAGAATTGAT TGGTGTAGCA 
AATCAGAAAA TGGTTCAGTT GTTGGCTGAC AATATAAATT CAGGGAATCC TTCTTCAATG 
TTAAAGATGG TGGAAGATTT GATAGCTGTT GGTGAAAAGG AAAGCTACAC ATTGAAGCAG 
AAAGTAACTT CCCAAGTTGT CATTTCAGTT CTTGTCTCTT GCTGCTCTTC ATTAAAGGAA 
ACTCACTTTC CATTTGCATT GAAAGTCTTC AATTTGCTGG AAAGGAAAAT AAGAGAAAAT 
GAAGTTATTA CTTCGTTGGA AATCTCTTTT GAATGTCATT CTGAACTGTC AGACAAAGGA 
ATACCAGTGG AGATATGGAT TCAGTATGTA GAAAAGCTCA ACAGAGGGCA GATGATTGCT 
GTGGAAGATG CTGTTATGCT TGCATTTTCA TTGAAAAACT TTATTCAAGT CTTGAAGCCT 
CCTAAATCTT TTCTTAAAGA TGATAAGTGG TGGAATCCTG AACATATGAA AGAAGACAGC 
CGAGACTATC TGCACTTATT GATCCAGATA TTTGAGGTTA TTATTGCTGG TGCTGATGCC 
CTTTGCTTCA GAGTTCTGAT GAAACTTTTC TTAAAGGTAC ATTTAGAAGA TGTTTTTGAG 
TTATTCAAGT TCTTCTCACT TTTATGGACC TATAATTATA GCCTTTCAAA TCCACTTAAC 
TATAATGTGA AAACAATACT ACAGACTCAA GCTCTTTACA TAGGACGTGC AATGCTGGCA 
GGTCAGGCTG CACAGAAGAA AAAATACCTG GCATCAACAT CTTCTCCAGT GGTGATATGT 
TTACTCATAA ATTTGGGAAG TCCTATAAGA GAAGTACGAA GAGCTGCCAT TCTCTGCCTC 
CAGACCTTAC GTGAAGTAGA GTCTCCACTT TATCCTGTAA TACAACACCT AGTCCTAAAA 
GAAGAGGAGA TTATTTCAGA TGCTACATAT GTAGCACAGG ATTTGAATAC TTTATTTGAA 
GATCTACAAA CAGAAAAAAA GCCAAAATCT CACCAGACAT TAACTGAAGC CTTAAAACAT 
TTGCTTTATT GTGTACATAG CTGTCCATCC TATATTGCAA AAAACTTGAT GAAAATTCTT 
CAGGAAGTCA ATAGTGAGAC GGTGCTTTCT CAGCTGTTGC CCACAATTGA TCAGTTGCTA 
GAGAAAGTCC TAAAGGAACC CCCTACAGTC CTGAAGGATG AAGCTGTCAT TTTGCACCTT 
GCCCTTGGGA AATACAATGA GCATTCAGCT TCTCTCTTGC ACAAGGACCC ACAGAGTTTA 
GATATATTCC TCAAAGCTGT GCATACCACC AAGGAAGTCT TCGAAGGGAT GCCTACATTC 
CAGATCCTTG CCCTTGGGAA GATTACAAAA CCATTCTTTG CTGCTATATC AGATGACAAA 
GTTCAACAGA AGCTTCTGGG AATGCTGTTT GATTTACTTT TGAATTGCAA AAATACACTC 
TGTGCTCAGA CTGTTAGCAG TGTTTTTAAA ACAATTTCTG TTGATGCTGA ACAAATCCGA 
TTAGAACTAG AACCACCAGA GAAAAACAAG TCCTTGGGTA CAATTCAGCA AACAAGAAGA 
CAGAAAATGC AACAGAAAAA ACTACAAGAT ACAGAACCTG CACAAGATGT TGGCAGTGCT 
TACTGGCAGA GAGTAACCCT CATCTTAGAA TTACTGCAGC ACAAAAAGAA ACTCAAAAGT 
CCTCAGTTAT TAGTGCCATG TCTTTTTAAT TTGCTCTCCA GGTGTCTAGA AACTTTGCCA 
CCGGAGCAGG GAAATCTGGA ATATACCAAA CAGCTAATTC TTAGCTGTCT ACTTAATATC 
TGCCAGAAAC TATCCCCAGA TGGTTCTAAG ATCCCTAAAG GTATTCTTGA TGAAGAAAAA 
TTCAATGTTG AATTGTTAGT TCAGTGTGTG CGGGTTTCAG AGATGCCTCA GACACATCAC 
CATGCCCTCT TGCTTTTGGG TGCAGTTGCT GGAATATTTC CTGATAAAGT TTTGCACAAC 
ATTATGCCCA TCTTTACATT CATGGGAGCC AATGTTATGC GCCTTGATGA CACTTACAGT 
TTTCAAGTTA TCAACAAGAC AGTGAAAATG GTCATTCCTG CTCTTATCCA GGCAGACAGT 
GGTAAGTCTG CAGATGTCAC AAGGAACATA GAAGAAGTTG TGGTTAAAAT TATAAATGTG 
TTTGTGGATG CTCTACCTCA TGTTCCCGAA CATAGACGCC TGCCCATCCT TGTACAACTT 
GTTGATACTC TTGGTGCAGA GAAATTCCTC TGGATTCTCC TGCTGTTGCT TTTTGAACAG 
TATGTCACTA AAACAGTGTT GGTTGCTGCT CATGGAGAAA AGGATGCTAT CTTAGAGACT 
GACACTGAAT TTTGGATATC AGTCTCTTGT GAATTTACTG TCCAACAGCA GCTCCAAAGT 
TTAATGAATA TACTCAGATA CTTAACCAAA CTTCCAGAGA AAAAAGAAGG AGAAGAACCT 
CCCAAAACAG GATCTCACAA CTCCAAGACA CTGGAACAGA TGGTACAGGT TTTCAATGTG 
GACACTCATA CCAGCAAGCA GCTCCGACAT TTTACATTTT TGTCTGTGTC CTTTATGTCT 
CAACTCTTGG CTTCCCATAA TTTTATCAAA AAGGTCATGG AAAATGGTGG TACTGTGGCT 
TTACAAGGCC TTGAACAGAG ATTACTTGAA ACTGTTCTTG GTTATATAAA TTCTGTGGCA 
CAGTCAGTGG AGGAAAATGC AGACAAATTA ACTGCAAAGT TTTGGCGTGC TCTCTTGAGT 
AAAGCTTATG ATTTGCTTGA TAAAGTCAAT TCCTTATTGC CCACAGAAAC TTTTGTTCCT 
GTGATAAGAG GATTGCTGAC TAACCAACTG CCTTCTGTCC GTCGTAAAGC AATGGACCTC 
TTAAACAATA AATTGCATCA GTATGCAAAG TGGAAAAAGG GACAGGTGAC CCTTTTCTTG 
AAACTGGTTC CAGATCTCTT GGATATTGCA CAGCATAAAA TAAAGGAGAA AGAAGAAGAA 
CAGGCCATCA ACAGACAGAC TGCCTTGTAC AGTTTGAAAT TACTCTGCAA AAATTTTGGC 
ACAGAAAATT CAGAGGTTTT TATCCCTGTA TTGAGTGCAG CCATTAACCT GATCAATCCA 
GAGGTGAAGG ATGAGAAGAA CGTATTGGGA AGTGCTTTGT TGTGTATAGC TGAGGTCACC 
TACGTACTGG GAGCATTAGC AATACCTCAT CTTCCCAGAC TTATGCCTGC ATTATTGACT 
ACCCTGGAGA ATACGAACGA GCTGATCTCA AGTGAGGTTT ACTTGCTTAG TGCATTGGCA 
GCCCTTCAGA AAGTTGTTGA AACTCTTCCA CATTTTCTCA GCCCATACCT ACAGGGTATT 
TTGATACAGG CAATTCGTTG GGAGACAGTT GTGAAGGAGA TGGGTCCTAC TTCCCAGGCA 
ACTCTCCGAG TGACATCCCT GAAAGCAACA CTGGCTACTA CACTTTCTTC AAGAGTTTTA 
CTGTCAGCAC TCAACACAGT TTACAAGCAA ATTGGAAAGA ACTGGAAGAA TCGAATAGGT 
CCATTTATGA GCCTCCTTCA AGAGCACATT GCAGTAATGG AGAAGGAAGA CCTCAACTCT 
CATCAGTCTC AGTTAACAGC ATTTTTCTTG GAAGCCTTGA ACTTCAGATC ATTACATTCA 
GAGGATAACC TAGAAGAAGT TGGTCAAACT GAAGGTTGTA TAGTTAGCTG TCTAATGAGC 
ATGGTTATGA AACTTTCTGA AGTTACTTTC AGACCTCTCT TCTTTAAGCT ATTTGATTGG 
GCCAGAACAG AAGATGCTCC AAAGGACAGG CTGCTGACAT TCTGCAACTT GGCAGATTGT 
ATTGCTGAAA AATTGAAAGG CCTTTTTACC CTATTTGCTG GTCATTTAGT GAAACCTTTT 
GCTGACACTT TGAACCAAAT CAACATCTCT AAAACAGACG AAGCCTTTTT TGACTCTGGA 
AATGAACCTG AAAAGAGCTG TTTGTTACTG CAGTCTATAT TGGATTGTCT GCACAAAATC 
TTTCTTTTTG ACACTCAGCA TTTTGTAAGC AAAGAGAGAG CTGAAGCACT GATGATGCCT 
CTGGTGGATC AGCTGGAAAA CATGCTTGGA GGAGAAGAGA CATTTCAGGA AAGAGTGACG 
AAGCACCTAA TACCGTGTAT TGCTCAGTTT TCTGTAGCCA TGGCAGATGA TTCTCTTTGG 
AAACCATTGA ACTACCAAAT TCTCCTTAAG ATGCGGCATT CTTCACCTAA GGTTCGATTC 
GCTGCCTTGA TTACAGTTTT AGAGCTGGCT GAAAAATTAA AGGAGAACTA CATCGTGCTT 
CTCCCTGAAT CCATTCCTTT CTTAGCAGAG CTGATGGAAG ATGAATGTGA AGAAGTGGAA 
AATCAGTGCC AGAAGACTAT TCAACAACTG GAAGTCATCT TGGGAGAGCC ACTCCAGAGC 
TATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012403412.1
CDS	129..6557
Translation	MTSLSQQLQRLALPQNDPSLLARHEVASLLFDCKEAASIDRDTIFAIGCTGLEELIGIDPSFQKFESSLFSQLAKSLERSVQTKAVNKQLDENISLFLIHLSPYFMLKPAQKCLEWLIHRFHIHLYNQDSLIGCVLPYHETKIFVRVIQLLKISSPTHKWNWLEPVKMSGVHLAKGTLVTHCYKDLGFMDFICSLVTKSVKVFSEYPGSSAQLRVLLTFYASTIVSALVSANDISDSLLAKLFPYIQKGLKSSLPDYRAATYMIICQVSVKVTMEDTFVHSLASQIGKTLTKIPSLIKNGLGCLIVLLQRQKVEGLGKKLFAHLCNVPDLVSLLHEISVTYDISPLLHYMLPHLVVTIVHHVTEEETEELDSHIYARLLEDILKDISLENNLDHLLASTLLEEYISYSSRKEIDSKEISLLNEQLLPLIRLLESKYPKTLDLVLEDHLKDVTDPQKQEFFHQFISLSTSGGKFQFLADSDSCLTLSLNHPLAPVRLLAVNHLKNIMETSKDNIDASFIKEAIIMRLGDDNLDVVLSAMNAFVVFKKEFSPEETVSSLLRLFQRAELSQDGAWYKILELAADTLIKEEILNGNDELLNQVVIHLLPFMVVTNDNSKSQEIKMATYLSKSGICSLHPILKGWKEALEKVIKTTKPTELIGVANQKMVQLLADNINSGNPSSMLKMVEDLIAVGEKESYTLKQKVTSQVVISVLVSCCSSLKETHFPFALKVFNLLERKIRENEVITSLEISFECHSELSDKGIPVEIWIQYVEKLNRGQMIAVEDAVMLAFSLKNFIQVLKPPKSFLKDDKWWNPEHMKEDSRDYLHLLIQIFEVIIAGADALCFRVLMKLFLKVHLEDVFELFKFFSLLWTYNYSLSNPLNYNVKTILQTQALYIGRAMLAGQAAQKKKYLASTSSPVVICLLINLGSPIREVRRAAILCLQTLREVESPLYPVIQHLVLKEEEIISDATYVAQDLNTLFEDLQTEKKPKSHQTLTEALKHLLYCVHSCPSYIAKNLMKILQEVNSETVLSQLLPTIDQLLEKVLKEPPTVLKDEAVILHLALGKYNEHSASLLHKDPQSLDIFLKAVHTTKEVFEGMPTFQILALGKITKPFFAAISDDKVQQKLLGMLFDLLLNCKNTLCAQTVSSVFKTISVDAEQIRLELEPPEKNKSLGTIQQTRRQKMQQKKLQDTEPAQDVGSAYWQRVTLILELLQHKKKLKSPQLLVPCLFNLLSRCLETLPPEQGNLEYTKQLILSCLLNICQKLSPDGSKIPKGILDEEKFNVELLVQCVRVSEMPQTHHHALLLLGAVAGIFPDKVLHNIMPIFTFMGANVMRLDDTYSFQVINKTVKMVIPALIQADSGKSADVTRNIEEVVVKIINVFVDALPHVPEHRRLPILVQLVDTLGAEKFLWILLLLLFEQYVTKTVLVAAHGEKDAILETDTEFWISVSCEFTVQQQLQSLMNILRYLTKLPEKKEGEEPPKTGSHNSKTLEQMVQVFNVDTHTSKQLRHFTFLSVSFMSQLLASHNFIKKVMENGGTVALQGLEQRLLETVLGYINSVAQSVEENADKLTAKFWRALLSKAYDLLDKVNSLLPTETFVPVIRGLLTNQLPSVRRKAMDLLNNKLHQYAKWKKGQVTLFLKLVPDLLDIAQHKIKEKEEEQAINRQTALYSLKLLCKNFGTENSEVFIPVLSAAINLINPEVKDEKNVLGSALLCIAEVTYVLGALAIPHLPRLMPALLTTLENTNELISSEVYLLSALAALQKVVETLPHFLSPYLQGILIQAIRWETVVKEMGPTSQATLRVTSLKATLATTLSSRVLLSALNTVYKQIGKNWKNRIGPFMSLLQEHIAVMEKEDLNSHQSQLTAFFLEALNFRSLHSEDNLEEVGQTEGCIVSCLMSMVMKLSEVTFRPLFFKLFDWARTEDAPKDRLLTFCNLADCIAEKLKGLFTLFAGHLVKPFADTLNQINISKTDEAFFDSGNEPEKSCLLLQSILDCLHKIFLFDTQHFVSKERAEALMMPLVDQLENMLGGEETFQERVTKHLIPCIAQFSVAMADDSLWKPLNYQILLKMRHSSPKVRFAALITVLELAEKLKENYIVLLPESIPFLAELMEDECEEVENQCQKTIQQLEVILGEPLQSYF

Target ORF information:

RefSeq Version	XM_012547958.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012547958.3

ORF Insert Sequence:

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAAC TGAGGAACTG GACAGTCATA TATATGCTAG ACTCTTAGAA 
GATATACTTA AAGATATATC ATTGGAAAAC AACTTAGACC ACTTGTTGGC TAGCACTCTT 
CTTGAAGAAT ATATTTCATA TAGTTCAAGG AAAGAAATAG ATTCTAAGGA GATATCACTA 
CTCAATGAAC AACTCCTTCC TCTCATTAGA CTTTTGGAAA GCAAATACCC TAAGACATTA 
GATTTGGTAT TGGAAGACCA TCTGAAGGAT GTTACAGATC CCCAAAAGCA AGAATTTTTT 
CATCAATTTA TTTCTCTCTC CACAAGTGGA GGAAAGTTTC AGTTTTTAGC AGATTCAGAT 
TCTTGCCTCA CGCTGAGCTT GAACCATCCA CTGGCTCCAG TGAGACTTTT GGCAGTTAAC 
CATCTGAAAA ATATTATGGA AACATCAAAG GACAATATTG ATGCTTCTTT CATAAAAGAA 
GCTATTATAA TGCGTCTTGG TGATGATAAT TTAGATGTTG TTCTGTCAGC CATGAATGCT 
TTTGTGGTTT TCAAAAAAGA GTTTAGCCCA GAAGAAACAG TTTCAAGTCT TTTGAGACTT 
TTTCAAAGAG CAGAACTTTC ACAGGATGGG GCATGGTACA AAATACTTGA ATTAGCAGCT 
GACACTCTAA TTAAAGAAGA GATTCTGAAT GGAAATGATG AGTTACTAAA TCAAGTGGTC 
ATACATTTGC TGCCATTTAT GGTTGTTACC AATGACAATT CAAAATCCCA AGAAATAAAA 
ATGGCTACTT ATCTGTCAAA ATCAGGAATT TGCTCTTTGC ACCCTATACT GAAAGGATGG 
AAAGAAGCTC TTGAAAAAGT GATTAAAACC ACAAAGCCAA CAGAATTGAT TGGTGTAGCA 
AATCAGAAAA TGGTTCAGTT GTTGGCTGAC AATATAAATT CAGGGAATCC TTCTTCAATG 
TTAAAGATGG TGGAAGATTT GATAGCTGTT GGTGAAAAGG AAAGCTACAC ATTGAAGCAG 
AAAGTAACTT CCCAAGTTGT CATTTCAGTT CTTGTCTCTT GCTGCTCTTC ATTAAAGGAA 
ACTCACTTTC CATTTGCATT GAAAGTCTTC AATTTGCTGG AAAGGAAAAT AAGAGAAAAT 
GAAGTTATTA CTTCGTTGGA AATCTCTTTT GAATGTCATT CTGAACTGTC AGACAAAGGA 
ATACCAGTGG AGATATGGAT TCAGTATGTA GAAAAGCTCA ACAGAGGGCA GATGATTGCT 
GTGGAAGATG CTGTTATGCT TGCATTTTCA TTGAAAAACT TTATTCAAGT CTTGAAGCCT 
CCTAAATCTT TTCTTAAAGA TGATAAGTGG TGGAATCCTG AACATATGAA AGAAGACAGC 
CGAGACTATC TGCACTTATT GATCCAGATA TTTGAGGTTA TTATTGCTGG TGCTGATGCC 
CTTTGCTTCA GAGTTCTGAT GAAACTTTTC TTAAAGGTAC ATTTAGAAGA TGTTTTTGAG 
TTATTCAAGT TCTTCTCACT TTTATGGACC TATAATTATA GCCTTTCAAA TCCACTTAAC 
TATAATGTGA AAACAATACT ACAGACTCAA GCTCTTTACA TAGGACGTGC AATGCTGGCA 
GGTCAGGCTG CACAGAAGAA AAAATACCTG GCATCAACAT CTTCTCCAGT GGTGATATGT 
TTACTCATAA ATTTGGGAAG TCCTATAAGA GAAGTACGAA GAGCTGCCAT TCTCTGCCTC 
CAGACCTTAC GTGAAGTAGA GTCTCCACTT TATCCTGTAA TACAACACCT AGTCCTAAAA 
GAAGAGGAGA TTATTTCAGA TGCTACATAT GTAGCACAGG ATTTGAATAC TTTATTTGAA 
GATCTACAAA CAGAAAAAAA GCCAAAATCT CACCAGACAT TAACTGAAGC CTTAAAACAT 
TTGCTTTATT GTGTACATAG CTGTCCATCC TATATTGCAA AAAACTTGAT GAAAATTCTT 
CAGGAAGTCA ATAGTGAGAC GGTGCTTTCT CAGCTGTTGC CCACAATTGA TCAGTTGCTA 
GAGAAAGTCC TAAAGGAACC CCCTACAGTC CTGAAGGATG AAGCTGTCAT TTTGCACCTT 
GCCCTTGGGA AATACAATGA GCATTCAGCT TCTCTCTTGC ACAAGGACCC ACAGAGTTTA 
GATATATTCC TCAAAGCTGT GCATACCACC AAGGAAGTCT TCGAAGGGAT GCCTACATTC 
CAGATCCTTG CCCTTGGGAA GATTACAAAA CCATTCTTTG CTGCTATATC AGATGACAAA 
GTTCAACAGA AGCTTCTGGG AATGCTGTTT GATTTACTTT TGAATTGCAA AAATACACTC 
TGTGCTCAGA CTGTTAGCAG TGTTTTTAAA ACAATTTCTG TTGATGCTGA ACAAATCCGA 
TTAGAACTAG AACCACCAGA GAAAAACAAG TCCTTGGGTA CAATTCAGCA AACAAGAAGA 
CAGAAAATGC AACAGAAAAA ACTACAAGAT ACAGAACCTG CACAAGATGT TGGCAGTGCT 
TACTGGCAGA GAGTAACCCT CATCTTAGAA TTACTGCAGC ACAAAAAGAA ACTCAAAAGT 
CCTCAGTTAT TAGTGCCATG TCTTTTTAAT TTGCTCTCCA GGTGTCTAGA AACTTTGCCA 
CCGGAGCAGG GAAATCTGGA ATATACCAAA CAGCTAATTC TTAGCTGTCT ACTTAATATC 
TGCCAGAAAC TATCCCCAGA TGGTTCTAAG ATCCCTAAAG GTATTCTTGA TGAAGAAAAA 
TTCAATGTTG AATTGTTAGT TCAGTGTGTG CGGGTTTCAG AGATGCCTCA GACACATCAC 
CATGCCCTCT TGCTTTTGGG TGCAGTTGCT GGAATATTTC CTGATAAAGT TTTGCACAAC 
ATTATGCCCA TCTTTACATT CATGGGAGCC AATGTTATGC GCCTTGATGA CACTTACAGT 
TTTCAAGTTA TCAACAAGAC AGTGAAAATG GTCATTCCTG CTCTTATCCA GGCAGACAGT 
GGTAAGTCTG CAGATGTCAC AAGGAACATA GAAGAAGTTG TGGTTAAAAT TATAAATGTG 
TTTGTGGATG CTCTACCTCA TGTTCCCGAA CATAGACGCC TGCCCATCCT TGTACAACTT 
GTTGATACTC TTGGTGCAGA GAAATTCCTC TGGATTCTCC TGCTGTTGCT TTTTGAACAG 
TATGTCACTA AAACAGTGTT GGTTGCTGCT CATGGAGAAA AGGATGCTAT CTTAGAGACT 
GACACTGAAT TTTGGATATC AGTCTCTTGT GAATTTACTG TCCAACAGCA GCTCCAAAGT 
TTAATGAATA TACTCAGATA CTTAACCAAA CTTCCAGAGA AAAAAGAAGG AGAAGAACCT 
CCCAAAACAG GATCTCACAA CTCCAAGACA CTGGAACAGA TGGTACAGGT TTTCAATGTG 
GACACTCATA CCAGCAAGCA GCTCCGACAT TTTACATTTT TGTCTGTGTC CTTTATGTCT 
CAACTCTTGG CTTCCCATAA TTTTATCAAA AAGGTCATGG AAAATGGTGG TACTGTGGCT 
TTACAAGGCC TTGAACAGAG ATTACTTGAA ACTGTTCTTG GTTATATAAA TTCTGTGGCA 
CAGTCAGTGG AGGAAAATGC AGACAAATTA ACTGCAAAGT TTTGGCGTGC TCTCTTGAGT 
AAAGCTTATG ATTTGCTTGA TAAAGTCAAT TCCTTATTGC CCACAGAAAC TTTTGTTCCT 
GTGATAAGAG GATTGCTGAC TAACCAACTG CCTTCTGTCC GTCGTAAAGC AATGGACCTC 
TTAAACAATA AATTGCATCA GTATGCAAAG TGGAAAAAGG GACAGGTGAC CCTTTTCTTG 
AAACTGGTTC CAGATCTCTT GGATATTGCA CAGCATAAAA TAAAGGAGAA AGAAGAAGAA 
CAGGCCATCA ACAGACAGAC TGCCTTGTAC AGTTTGAAAT TACTCTGCAA AAATTTTGGC 
ACAGAAAATT CAGAGGTTTT TATCCCTGTA TTGAGTGCAG CCATTAACCT GATCAATCCA 
GAGGTGAAGG ATGAGAAGAA CGTATTGGGA AGTGCTTTGT TGTGTATAGC TGAGGTCACC 
TACGTACTGG GAGCATTAGC AATACCTCAT CTTCCCAGAC TTATGCCTGC ATTATTGACT 
ACCCTGGAGA ATACGAACGA GCTGATCTCA AGTGAGGTTT ACTTGCTTAG TGCATTGGCA 
GCCCTTCAGA AAGTTGTTGA AACTCTTCCA CATTTTCTCA GCCCATACCT ACAGGGTATT 
TTGATACAGG CAATTCGTTG GGAGACAGTT GTGAAGGAGA TGGGTCCTAC TTCCCAGGCA 
ACTCTCCGAG TGACATCCCT GAAAGCAACA CTGGCTACTA CACTTTCTTC AAGAGTTTTA 
CTGTCAGCAC TCAACACAGT TTACAAGCAA ATTGGAAAGA ACTGGAAGAA TCGAATAGGT 
CCATTTATGA GCCTCCTTCA AGAGCACATT GCAGTAATGG AGAAGGAAGA CCTCAACTCT 
CATCAGTCTC AGTTAACAGC ATTTTTCTTG GAAGCCTTGA ACTTCAGATC ATTACATTCA 
GAGGATAACC TAGAAGAAGT TGGTCAAACT GAAGGTTGTA TAGTTAGCTG TCTAATGAGC 
ATGGTTATGA AACTTTCTGA AGTTACTTTC AGACCTCTCT TCTTTAAGCT ATTTGATTGG 
GCCAGAACAG AAGATGCTCC AAAGGACAGG CTGCTGACAT TCTGCAACTT GGCAGATTGT 
ATTGCTGAAA AATTGAAAGG CCTTTTTACC CTATTTGCTG GTCATTTAGT GAAACCTTTT 
GCTGACACTT TGAACCAAAT CAACATCTCT AAAACAGACG AAGCCTTTTT TGACTCTGGA 
AATGAACCTG AAAAGAGCTG TTTGTTACTG CAGTCTATAT TGGATTGTCT GCACAAAATC 
TTTCTTTTTG ACACTCAGCA TTTTGTAAGC AAAGAGAGAG CTGAAGCACT GATGATGCCT 
CTGGTGGATC AGCTGGAAAA CATGCTTGGA GGAGAAGAGA CATTTCAGGA AAGAGTGACG 
AAGCACCTAA TACCGTGTAT TGCTCAGTTT TCTGTAGCCA TGGCAGATGA TTCTCTTTGG 
AAACCATTGA ACTACCAAAT TCTCCTTAAG ATGCGGCATT CTTCACCTAA GGTTCGATTC 
GCTGCCTTGA TTACAGTTTT AGAGCTGGCT GAAAAATTAA AGGAGAACTA CATCGTGCTT 
CTCCCTGAAT CCATTCCTTT CTTAGCAGAG CTGATGGAAG ATGAATGTGA AGAAGTGGAA 
AATCAGTGCC AGAAGACTAT TCAACAACTG GAAGTCATCT TGGGAGAGCC ACTCCAGAGC 
TATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR1 ( XM_012547958.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012403412.1 Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), mRNA.

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CloneID	OSa129748
Clone ID Related Accession (Same CDS sequence)	XM_012547958.3 , XM_012547958.1 , XM_012547958.2
Accession Version	XM_012547958.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAAC TGAGGAACTG GACAGTCATA TATATGCTAG ACTCTTAGAA 
GATATACTTA AAGATATATC ATTGGAAAAC AACTTAGACC ACTTGTTGGC TAGCACTCTT 
CTTGAAGAAT ATATTTCATA TAGTTCAAGG AAAGAAATAG ATTCTAAGGA GATATCACTA 
CTCAATGAAC AACTCCTTCC TCTCATTAGA CTTTTGGAAA GCAAATACCC TAAGACATTA 
GATTTGGTAT TGGAAGACCA TCTGAAGGAT GTTACAGATC CCCAAAAGCA AGAATTTTTT 
CATCAATTTA TTTCTCTCTC CACAAGTGGA GGAAAGTTTC AGTTTTTAGC AGATTCAGAT 
TCTTGCCTCA CGCTGAGCTT GAACCATCCA CTGGCTCCAG TGAGACTTTT GGCAGTTAAC 
CATCTGAAAA ATATTATGGA AACATCAAAG GACAATATTG ATGCTTCTTT CATAAAAGAA 
GCTATTATAA TGCGTCTTGG TGATGATAAT TTAGATGTTG TTCTGTCAGC CATGAATGCT 
TTTGTGGTTT TCAAAAAAGA GTTTAGCCCA GAAGAAACAG TTTCAAGTCT TTTGAGACTT 
TTTCAAAGAG CAGAACTTTC ACAGGATGGG GCATGGTACA AAATACTTGA ATTAGCAGCT 
GACACTCTAA TTAAAGAAGA GATTCTGAAT GGAAATGATG AGTTACTAAA TCAAGTGGTC 
ATACATTTGC TGCCATTTAT GGTTGTTACC AATGACAATT CAAAATCCCA AGAAATAAAA 
ATGGCTACTT ATCTGTCAAA ATCAGGAATT TGCTCTTTGC ACCCTATACT GAAAGGATGG 
AAAGAAGCTC TTGAAAAAGT GATTAAAACC ACAAAGCCAA CAGAATTGAT TGGTGTAGCA 
AATCAGAAAA TGGTTCAGTT GTTGGCTGAC AATATAAATT CAGGGAATCC TTCTTCAATG 
TTAAAGATGG TGGAAGATTT GATAGCTGTT GGTGAAAAGG AAAGCTACAC ATTGAAGCAG 
AAAGTAACTT CCCAAGTTGT CATTTCAGTT CTTGTCTCTT GCTGCTCTTC ATTAAAGGAA 
ACTCACTTTC CATTTGCATT GAAAGTCTTC AATTTGCTGG AAAGGAAAAT AAGAGAAAAT 
GAAGTTATTA CTTCGTTGGA AATCTCTTTT GAATGTCATT CTGAACTGTC AGACAAAGGA 
ATACCAGTGG AGATATGGAT TCAGTATGTA GAAAAGCTCA ACAGAGGGCA GATGATTGCT 
GTGGAAGATG CTGTTATGCT TGCATTTTCA TTGAAAAACT TTATTCAAGT CTTGAAGCCT 
CCTAAATCTT TTCTTAAAGA TGATAAGTGG TGGAATCCTG AACATATGAA AGAAGACAGC 
CGAGACTATC TGCACTTATT GATCCAGATA TTTGAGGTTA TTATTGCTGG TGCTGATGCC 
CTTTGCTTCA GAGTTCTGAT GAAACTTTTC TTAAAGGTAC ATTTAGAAGA TGTTTTTGAG 
TTATTCAAGT TCTTCTCACT TTTATGGACC TATAATTATA GCCTTTCAAA TCCACTTAAC 
TATAATGTGA AAACAATACT ACAGACTCAA GCTCTTTACA TAGGACGTGC AATGCTGGCA 
GGTCAGGCTG CACAGAAGAA AAAATACCTG GCATCAACAT CTTCTCCAGT GGTGATATGT 
TTACTCATAA ATTTGGGAAG TCCTATAAGA GAAGTACGAA GAGCTGCCAT TCTCTGCCTC 
CAGACCTTAC GTGAAGTAGA GTCTCCACTT TATCCTGTAA TACAACACCT AGTCCTAAAA 
GAAGAGGAGA TTATTTCAGA TGCTACATAT GTAGCACAGG ATTTGAATAC TTTATTTGAA 
GATCTACAAA CAGAAAAAAA GCCAAAATCT CACCAGACAT TAACTGAAGC CTTAAAACAT 
TTGCTTTATT GTGTACATAG CTGTCCATCC TATATTGCAA AAAACTTGAT GAAAATTCTT 
CAGGAAGTCA ATAGTGAGAC GGTGCTTTCT CAGCTGTTGC CCACAATTGA TCAGTTGCTA 
GAGAAAGTCC TAAAGGAACC CCCTACAGTC CTGAAGGATG AAGCTGTCAT TTTGCACCTT 
GCCCTTGGGA AATACAATGA GCATTCAGCT TCTCTCTTGC ACAAGGACCC ACAGAGTTTA 
GATATATTCC TCAAAGCTGT GCATACCACC AAGGAAGTCT TCGAAGGGAT GCCTACATTC 
CAGATCCTTG CCCTTGGGAA GATTACAAAA CCATTCTTTG CTGCTATATC AGATGACAAA 
GTTCAACAGA AGCTTCTGGG AATGCTGTTT GATTTACTTT TGAATTGCAA AAATACACTC 
TGTGCTCAGA CTGTTAGCAG TGTTTTTAAA ACAATTTCTG TTGATGCTGA ACAAATCCGA 
TTAGAACTAG AACCACCAGA GAAAAACAAG TCCTTGGGTA CAATTCAGCA AACAAGAAGA 
CAGAAAATGC AACAGAAAAA ACTACAAGAT ACAGAACCTG CACAAGATGT TGGCAGTGCT 
TACTGGCAGA GAGTAACCCT CATCTTAGAA TTACTGCAGC ACAAAAAGAA ACTCAAAAGT 
CCTCAGTTAT TAGTGCCATG TCTTTTTAAT TTGCTCTCCA GGTGTCTAGA AACTTTGCCA 
CCGGAGCAGG GAAATCTGGA ATATACCAAA CAGCTAATTC TTAGCTGTCT ACTTAATATC 
TGCCAGAAAC TATCCCCAGA TGGTTCTAAG ATCCCTAAAG GTATTCTTGA TGAAGAAAAA 
TTCAATGTTG AATTGTTAGT TCAGTGTGTG CGGGTTTCAG AGATGCCTCA GACACATCAC 
CATGCCCTCT TGCTTTTGGG TGCAGTTGCT GGAATATTTC CTGATAAAGT TTTGCACAAC 
ATTATGCCCA TCTTTACATT CATGGGAGCC AATGTTATGC GCCTTGATGA CACTTACAGT 
TTTCAAGTTA TCAACAAGAC AGTGAAAATG GTCATTCCTG CTCTTATCCA GGCAGACAGT 
GGTAAGTCTG CAGATGTCAC AAGGAACATA GAAGAAGTTG TGGTTAAAAT TATAAATGTG 
TTTGTGGATG CTCTACCTCA TGTTCCCGAA CATAGACGCC TGCCCATCCT TGTACAACTT 
GTTGATACTC TTGGTGCAGA GAAATTCCTC TGGATTCTCC TGCTGTTGCT TTTTGAACAG 
TATGTCACTA AAACAGTGTT GGTTGCTGCT CATGGAGAAA AGGATGCTAT CTTAGAGACT 
GACACTGAAT TTTGGATATC AGTCTCTTGT GAATTTACTG TCCAACAGCA GCTCCAAAGT 
TTAATGAATA TACTCAGATA CTTAACCAAA CTTCCAGAGA AAAAAGAAGG AGAAGAACCT 
CCCAAAACAG GATCTCACAA CTCCAAGACA CTGGAACAGA TGGTACAGGT TTTCAATGTG 
GACACTCATA CCAGCAAGCA GCTCCGACAT TTTACATTTT TGTCTGTGTC CTTTATGTCT 
CAACTCTTGG CTTCCCATAA TTTTATCAAA AAGGTCATGG AAAATGGTGG TACTGTGGCT 
TTACAAGGCC TTGAACAGAG ATTACTTGAA ACTGTTCTTG GTTATATAAA TTCTGTGGCA 
CAGTCAGTGG AGGAAAATGC AGACAAATTA ACTGCAAAGT TTTGGCGTGC TCTCTTGAGT 
AAAGCTTATG ATTTGCTTGA TAAAGTCAAT TCCTTATTGC CCACAGAAAC TTTTGTTCCT 
GTGATAAGAG GATTGCTGAC TAACCAACTG CCTTCTGTCC GTCGTAAAGC AATGGACCTC 
TTAAACAATA AATTGCATCA GTATGCAAAG TGGAAAAAGG GACAGGTGAC CCTTTTCTTG 
AAACTGGTTC CAGATCTCTT GGATATTGCA CAGCATAAAA TAAAGGAGAA AGAAGAAGAA 
CAGGCCATCA ACAGACAGAC TGCCTTGTAC AGTTTGAAAT TACTCTGCAA AAATTTTGGC 
ACAGAAAATT CAGAGGTTTT TATCCCTGTA TTGAGTGCAG CCATTAACCT GATCAATCCA 
GAGGTGAAGG ATGAGAAGAA CGTATTGGGA AGTGCTTTGT TGTGTATAGC TGAGGTCACC 
TACGTACTGG GAGCATTAGC AATACCTCAT CTTCCCAGAC TTATGCCTGC ATTATTGACT 
ACCCTGGAGA ATACGAACGA GCTGATCTCA AGTGAGGTTT ACTTGCTTAG TGCATTGGCA 
GCCCTTCAGA AAGTTGTTGA AACTCTTCCA CATTTTCTCA GCCCATACCT ACAGGGTATT 
TTGATACAGG CAATTCGTTG GGAGACAGTT GTGAAGGAGA TGGGTCCTAC TTCCCAGGCA 
ACTCTCCGAG TGACATCCCT GAAAGCAACA CTGGCTACTA CACTTTCTTC AAGAGTTTTA 
CTGTCAGCAC TCAACACAGT TTACAAGCAA ATTGGAAAGA ACTGGAAGAA TCGAATAGGT 
CCATTTATGA GCCTCCTTCA AGAGCACATT GCAGTAATGG AGAAGGAAGA CCTCAACTCT 
CATCAGTCTC AGTTAACAGC ATTTTTCTTG GAAGCCTTGA ACTTCAGATC ATTACATTCA 
GAGGATAACC TAGAAGAAGT TGGTCAAACT GAAGGTTGTA TAGTTAGCTG TCTAATGAGC 
ATGGTTATGA AACTTTCTGA AGTTACTTTC AGACCTCTCT TCTTTAAGCT ATTTGATTGG 
GCCAGAACAG AAGATGCTCC AAAGGACAGG CTGCTGACAT TCTGCAACTT GGCAGATTGT 
ATTGCTGAAA AATTGAAAGG CCTTTTTACC CTATTTGCTG GTCATTTAGT GAAACCTTTT 
GCTGACACTT TGAACCAAAT CAACATCTCT AAAACAGACG AAGCCTTTTT TGACTCTGGA 
AATGAACCTG AAAAGAGCTG TTTGTTACTG CAGTCTATAT TGGATTGTCT GCACAAAATC 
TTTCTTTTTG ACACTCAGCA TTTTGTAAGC AAAGAGAGAG CTGAAGCACT GATGATGCCT 
CTGGTGGATC AGCTGGAAAA CATGCTTGGA GGAGAAGAGA CATTTCAGGA AAGAGTGACG 
AAGCACCTAA TACCGTGTAT TGCTCAGTTT TCTGTAGCCA TGGCAGATGA TTCTCTTTGG 
AAACCATTGA ACTACCAAAT TCTCCTTAAG ATGCGGCATT CTTCACCTAA GGTTCGATTC 
GCTGCCTTGA TTACAGTTTT AGAGCTGGCT GAAAAATTAA AGGAGAACTA CATCGTGCTT 
CTCCCTGAAT CCATTCCTTT CTTAGCAGAG CTGATGGAAG ATGAATGTGA AGAAGTGGAA 
AATCAGTGCC AGAAGACTAT TCAACAACTG GAAGTCATCT TGGGAGAGCC ACTCCAGAGC 
TATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012403412.1
CDS	87..6515
Translation	MTSLSQQLQRLALPQNDPSLLARHEVASLLFDCKEAASIDRDTIFAIGCTGLEELIGIDPSFQKFESSLFSQLAKSLERSVQTKAVNKQLDENISLFLIHLSPYFMLKPAQKCLEWLIHRFHIHLYNQDSLIGCVLPYHETKIFVRVIQLLKISSPTHKWNWLEPVKMSGVHLAKGTLVTHCYKDLGFMDFICSLVTKSVKVFSEYPGSSAQLRVLLTFYASTIVSALVSANDISDSLLAKLFPYIQKGLKSSLPDYRAATYMIICQVSVKVTMEDTFVHSLASQIGKTLTKIPSLIKNGLGCLIVLLQRQKVEGLGKKLFAHLCNVPDLVSLLHEISVTYDISPLLHYMLPHLVVTIVHHVTEEETEELDSHIYARLLEDILKDISLENNLDHLLASTLLEEYISYSSRKEIDSKEISLLNEQLLPLIRLLESKYPKTLDLVLEDHLKDVTDPQKQEFFHQFISLSTSGGKFQFLADSDSCLTLSLNHPLAPVRLLAVNHLKNIMETSKDNIDASFIKEAIIMRLGDDNLDVVLSAMNAFVVFKKEFSPEETVSSLLRLFQRAELSQDGAWYKILELAADTLIKEEILNGNDELLNQVVIHLLPFMVVTNDNSKSQEIKMATYLSKSGICSLHPILKGWKEALEKVIKTTKPTELIGVANQKMVQLLADNINSGNPSSMLKMVEDLIAVGEKESYTLKQKVTSQVVISVLVSCCSSLKETHFPFALKVFNLLERKIRENEVITSLEISFECHSELSDKGIPVEIWIQYVEKLNRGQMIAVEDAVMLAFSLKNFIQVLKPPKSFLKDDKWWNPEHMKEDSRDYLHLLIQIFEVIIAGADALCFRVLMKLFLKVHLEDVFELFKFFSLLWTYNYSLSNPLNYNVKTILQTQALYIGRAMLAGQAAQKKKYLASTSSPVVICLLINLGSPIREVRRAAILCLQTLREVESPLYPVIQHLVLKEEEIISDATYVAQDLNTLFEDLQTEKKPKSHQTLTEALKHLLYCVHSCPSYIAKNLMKILQEVNSETVLSQLLPTIDQLLEKVLKEPPTVLKDEAVILHLALGKYNEHSASLLHKDPQSLDIFLKAVHTTKEVFEGMPTFQILALGKITKPFFAAISDDKVQQKLLGMLFDLLLNCKNTLCAQTVSSVFKTISVDAEQIRLELEPPEKNKSLGTIQQTRRQKMQQKKLQDTEPAQDVGSAYWQRVTLILELLQHKKKLKSPQLLVPCLFNLLSRCLETLPPEQGNLEYTKQLILSCLLNICQKLSPDGSKIPKGILDEEKFNVELLVQCVRVSEMPQTHHHALLLLGAVAGIFPDKVLHNIMPIFTFMGANVMRLDDTYSFQVINKTVKMVIPALIQADSGKSADVTRNIEEVVVKIINVFVDALPHVPEHRRLPILVQLVDTLGAEKFLWILLLLLFEQYVTKTVLVAAHGEKDAILETDTEFWISVSCEFTVQQQLQSLMNILRYLTKLPEKKEGEEPPKTGSHNSKTLEQMVQVFNVDTHTSKQLRHFTFLSVSFMSQLLASHNFIKKVMENGGTVALQGLEQRLLETVLGYINSVAQSVEENADKLTAKFWRALLSKAYDLLDKVNSLLPTETFVPVIRGLLTNQLPSVRRKAMDLLNNKLHQYAKWKKGQVTLFLKLVPDLLDIAQHKIKEKEEEQAINRQTALYSLKLLCKNFGTENSEVFIPVLSAAINLINPEVKDEKNVLGSALLCIAEVTYVLGALAIPHLPRLMPALLTTLENTNELISSEVYLLSALAALQKVVETLPHFLSPYLQGILIQAIRWETVVKEMGPTSQATLRVTSLKATLATTLSSRVLLSALNTVYKQIGKNWKNRIGPFMSLLQEHIAVMEKEDLNSHQSQLTAFFLEALNFRSLHSEDNLEEVGQTEGCIVSCLMSMVMKLSEVTFRPLFFKLFDWARTEDAPKDRLLTFCNLADCIAEKLKGLFTLFAGHLVKPFADTLNQINISKTDEAFFDSGNEPEKSCLLLQSILDCLHKIFLFDTQHFVSKERAEALMMPLVDQLENMLGGEETFQERVTKHLIPCIAQFSVAMADDSLWKPLNYQILLKMRHSSPKVRFAALITVLELAEKLKENYIVLLPESIPFLAELMEDECEEVENQCQKTIQQLEVILGEPLQSYF

Target ORF information:

RefSeq Version	XM_012547958.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012547958.1

ORF Insert Sequence:

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAAC TGAGGAACTG GACAGTCATA TATATGCTAG ACTCTTAGAA 
GATATACTTA AAGATATATC ATTGGAAAAC AACTTAGACC ACTTGTTGGC TAGCACTCTT 
CTTGAAGAAT ATATTTCATA TAGTTCAAGG AAAGAAATAG ATTCTAAGGA GATATCACTA 
CTCAATGAAC AACTCCTTCC TCTCATTAGA CTTTTGGAAA GCAAATACCC TAAGACATTA 
GATTTGGTAT TGGAAGACCA TCTGAAGGAT GTTACAGATC CCCAAAAGCA AGAATTTTTT 
CATCAATTTA TTTCTCTCTC CACAAGTGGA GGAAAGTTTC AGTTTTTAGC AGATTCAGAT 
TCTTGCCTCA CGCTGAGCTT GAACCATCCA CTGGCTCCAG TGAGACTTTT GGCAGTTAAC 
CATCTGAAAA ATATTATGGA AACATCAAAG GACAATATTG ATGCTTCTTT CATAAAAGAA 
GCTATTATAA TGCGTCTTGG TGATGATAAT TTAGATGTTG TTCTGTCAGC CATGAATGCT 
TTTGTGGTTT TCAAAAAAGA GTTTAGCCCA GAAGAAACAG TTTCAAGTCT TTTGAGACTT 
TTTCAAAGAG CAGAACTTTC ACAGGATGGG GCATGGTACA AAATACTTGA ATTAGCAGCT 
GACACTCTAA TTAAAGAAGA GATTCTGAAT GGAAATGATG AGTTACTAAA TCAAGTGGTC 
ATACATTTGC TGCCATTTAT GGTTGTTACC AATGACAATT CAAAATCCCA AGAAATAAAA 
ATGGCTACTT ATCTGTCAAA ATCAGGAATT TGCTCTTTGC ACCCTATACT GAAAGGATGG 
AAAGAAGCTC TTGAAAAAGT GATTAAAACC ACAAAGCCAA CAGAATTGAT TGGTGTAGCA 
AATCAGAAAA TGGTTCAGTT GTTGGCTGAC AATATAAATT CAGGGAATCC TTCTTCAATG 
TTAAAGATGG TGGAAGATTT GATAGCTGTT GGTGAAAAGG AAAGCTACAC ATTGAAGCAG 
AAAGTAACTT CCCAAGTTGT CATTTCAGTT CTTGTCTCTT GCTGCTCTTC ATTAAAGGAA 
ACTCACTTTC CATTTGCATT GAAAGTCTTC AATTTGCTGG AAAGGAAAAT AAGAGAAAAT 
GAAGTTATTA CTTCGTTGGA AATCTCTTTT GAATGTCATT CTGAACTGTC AGACAAAGGA 
ATACCAGTGG AGATATGGAT TCAGTATGTA GAAAAGCTCA ACAGAGGGCA GATGATTGCT 
GTGGAAGATG CTGTTATGCT TGCATTTTCA TTGAAAAACT TTATTCAAGT CTTGAAGCCT 
CCTAAATCTT TTCTTAAAGA TGATAAGTGG TGGAATCCTG AACATATGAA AGAAGACAGC 
CGAGACTATC TGCACTTATT GATCCAGATA TTTGAGGTTA TTATTGCTGG TGCTGATGCC 
CTTTGCTTCA GAGTTCTGAT GAAACTTTTC TTAAAGGTAC ATTTAGAAGA TGTTTTTGAG 
TTATTCAAGT TCTTCTCACT TTTATGGACC TATAATTATA GCCTTTCAAA TCCACTTAAC 
TATAATGTGA AAACAATACT ACAGACTCAA GCTCTTTACA TAGGACGTGC AATGCTGGCA 
GGTCAGGCTG CACAGAAGAA AAAATACCTG GCATCAACAT CTTCTCCAGT GGTGATATGT 
TTACTCATAA ATTTGGGAAG TCCTATAAGA GAAGTACGAA GAGCTGCCAT TCTCTGCCTC 
CAGACCTTAC GTGAAGTAGA GTCTCCACTT TATCCTGTAA TACAACACCT AGTCCTAAAA 
GAAGAGGAGA TTATTTCAGA TGCTACATAT GTAGCACAGG ATTTGAATAC TTTATTTGAA 
GATCTACAAA CAGAAAAAAA GCCAAAATCT CACCAGACAT TAACTGAAGC CTTAAAACAT 
TTGCTTTATT GTGTACATAG CTGTCCATCC TATATTGCAA AAAACTTGAT GAAAATTCTT 
CAGGAAGTCA ATAGTGAGAC GGTGCTTTCT CAGCTGTTGC CCACAATTGA TCAGTTGCTA 
GAGAAAGTCC TAAAGGAACC CCCTACAGTC CTGAAGGATG AAGCTGTCAT TTTGCACCTT 
GCCCTTGGGA AATACAATGA GCATTCAGCT TCTCTCTTGC ACAAGGACCC ACAGAGTTTA 
GATATATTCC TCAAAGCTGT GCATACCACC AAGGAAGTCT TCGAAGGGAT GCCTACATTC 
CAGATCCTTG CCCTTGGGAA GATTACAAAA CCATTCTTTG CTGCTATATC AGATGACAAA 
GTTCAACAGA AGCTTCTGGG AATGCTGTTT GATTTACTTT TGAATTGCAA AAATACACTC 
TGTGCTCAGA CTGTTAGCAG TGTTTTTAAA ACAATTTCTG TTGATGCTGA ACAAATCCGA 
TTAGAACTAG AACCACCAGA GAAAAACAAG TCCTTGGGTA CAATTCAGCA AACAAGAAGA 
CAGAAAATGC AACAGAAAAA ACTACAAGAT ACAGAACCTG CACAAGATGT TGGCAGTGCT 
TACTGGCAGA GAGTAACCCT CATCTTAGAA TTACTGCAGC ACAAAAAGAA ACTCAAAAGT 
CCTCAGTTAT TAGTGCCATG TCTTTTTAAT TTGCTCTCCA GGTGTCTAGA AACTTTGCCA 
CCGGAGCAGG GAAATCTGGA ATATACCAAA CAGCTAATTC TTAGCTGTCT ACTTAATATC 
TGCCAGAAAC TATCCCCAGA TGGTTCTAAG ATCCCTAAAG GTATTCTTGA TGAAGAAAAA 
TTCAATGTTG AATTGTTAGT TCAGTGTGTG CGGGTTTCAG AGATGCCTCA GACACATCAC 
CATGCCCTCT TGCTTTTGGG TGCAGTTGCT GGAATATTTC CTGATAAAGT TTTGCACAAC 
ATTATGCCCA TCTTTACATT CATGGGAGCC AATGTTATGC GCCTTGATGA CACTTACAGT 
TTTCAAGTTA TCAACAAGAC AGTGAAAATG GTCATTCCTG CTCTTATCCA GGCAGACAGT 
GGTAAGTCTG CAGATGTCAC AAGGAACATA GAAGAAGTTG TGGTTAAAAT TATAAATGTG 
TTTGTGGATG CTCTACCTCA TGTTCCCGAA CATAGACGCC TGCCCATCCT TGTACAACTT 
GTTGATACTC TTGGTGCAGA GAAATTCCTC TGGATTCTCC TGCTGTTGCT TTTTGAACAG 
TATGTCACTA AAACAGTGTT GGTTGCTGCT CATGGAGAAA AGGATGCTAT CTTAGAGACT 
GACACTGAAT TTTGGATATC AGTCTCTTGT GAATTTACTG TCCAACAGCA GCTCCAAAGT 
TTAATGAATA TACTCAGATA CTTAACCAAA CTTCCAGAGA AAAAAGAAGG AGAAGAACCT 
CCCAAAACAG GATCTCACAA CTCCAAGACA CTGGAACAGA TGGTACAGGT TTTCAATGTG 
GACACTCATA CCAGCAAGCA GCTCCGACAT TTTACATTTT TGTCTGTGTC CTTTATGTCT 
CAACTCTTGG CTTCCCATAA TTTTATCAAA AAGGTCATGG AAAATGGTGG TACTGTGGCT 
TTACAAGGCC TTGAACAGAG ATTACTTGAA ACTGTTCTTG GTTATATAAA TTCTGTGGCA 
CAGTCAGTGG AGGAAAATGC AGACAAATTA ACTGCAAAGT TTTGGCGTGC TCTCTTGAGT 
AAAGCTTATG ATTTGCTTGA TAAAGTCAAT TCCTTATTGC CCACAGAAAC TTTTGTTCCT 
GTGATAAGAG GATTGCTGAC TAACCAACTG CCTTCTGTCC GTCGTAAAGC AATGGACCTC 
TTAAACAATA AATTGCATCA GTATGCAAAG TGGAAAAAGG GACAGGTGAC CCTTTTCTTG 
AAACTGGTTC CAGATCTCTT GGATATTGCA CAGCATAAAA TAAAGGAGAA AGAAGAAGAA 
CAGGCCATCA ACAGACAGAC TGCCTTGTAC AGTTTGAAAT TACTCTGCAA AAATTTTGGC 
ACAGAAAATT CAGAGGTTTT TATCCCTGTA TTGAGTGCAG CCATTAACCT GATCAATCCA 
GAGGTGAAGG ATGAGAAGAA CGTATTGGGA AGTGCTTTGT TGTGTATAGC TGAGGTCACC 
TACGTACTGG GAGCATTAGC AATACCTCAT CTTCCCAGAC TTATGCCTGC ATTATTGACT 
ACCCTGGAGA ATACGAACGA GCTGATCTCA AGTGAGGTTT ACTTGCTTAG TGCATTGGCA 
GCCCTTCAGA AAGTTGTTGA AACTCTTCCA CATTTTCTCA GCCCATACCT ACAGGGTATT 
TTGATACAGG CAATTCGTTG GGAGACAGTT GTGAAGGAGA TGGGTCCTAC TTCCCAGGCA 
ACTCTCCGAG TGACATCCCT GAAAGCAACA CTGGCTACTA CACTTTCTTC AAGAGTTTTA 
CTGTCAGCAC TCAACACAGT TTACAAGCAA ATTGGAAAGA ACTGGAAGAA TCGAATAGGT 
CCATTTATGA GCCTCCTTCA AGAGCACATT GCAGTAATGG AGAAGGAAGA CCTCAACTCT 
CATCAGTCTC AGTTAACAGC ATTTTTCTTG GAAGCCTTGA ACTTCAGATC ATTACATTCA 
GAGGATAACC TAGAAGAAGT TGGTCAAACT GAAGGTTGTA TAGTTAGCTG TCTAATGAGC 
ATGGTTATGA AACTTTCTGA AGTTACTTTC AGACCTCTCT TCTTTAAGCT ATTTGATTGG 
GCCAGAACAG AAGATGCTCC AAAGGACAGG CTGCTGACAT TCTGCAACTT GGCAGATTGT 
ATTGCTGAAA AATTGAAAGG CCTTTTTACC CTATTTGCTG GTCATTTAGT GAAACCTTTT 
GCTGACACTT TGAACCAAAT CAACATCTCT AAAACAGACG AAGCCTTTTT TGACTCTGGA 
AATGAACCTG AAAAGAGCTG TTTGTTACTG CAGTCTATAT TGGATTGTCT GCACAAAATC 
TTTCTTTTTG ACACTCAGCA TTTTGTAAGC AAAGAGAGAG CTGAAGCACT GATGATGCCT 
CTGGTGGATC AGCTGGAAAA CATGCTTGGA GGAGAAGAGA CATTTCAGGA AAGAGTGACG 
AAGCACCTAA TACCGTGTAT TGCTCAGTTT TCTGTAGCCA TGGCAGATGA TTCTCTTTGG 
AAACCATTGA ACTACCAAAT TCTCCTTAAG ATGCGGCATT CTTCACCTAA GGTTCGATTC 
GCTGCCTTGA TTACAGTTTT AGAGCTGGCT GAAAAATTAA AGGAGAACTA CATCGTGCTT 
CTCCCTGAAT CCATTCCTTT CTTAGCAGAG CTGATGGAAG ATGAATGTGA AGAAGTGGAA 
AATCAGTGCC AGAAGACTAT TCAACAACTG GAAGTCATCT TGGGAGAGCC ACTCCAGAGC 
TATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR1 ( XM_012547958.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012403412.1 Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X2, mRNA.

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CloneID	OSa129748
Clone ID Related Accession (Same CDS sequence)	XM_012547958.3 , XM_012547958.1 , XM_012547958.2
Accession Version	XM_012547958.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012547958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAAC TGAGGAACTG GACAGTCATA TATATGCTAG ACTCTTAGAA 
GATATACTTA AAGATATATC ATTGGAAAAC AACTTAGACC ACTTGTTGGC TAGCACTCTT 
CTTGAAGAAT ATATTTCATA TAGTTCAAGG AAAGAAATAG ATTCTAAGGA GATATCACTA 
CTCAATGAAC AACTCCTTCC TCTCATTAGA CTTTTGGAAA GCAAATACCC TAAGACATTA 
GATTTGGTAT TGGAAGACCA TCTGAAGGAT GTTACAGATC CCCAAAAGCA AGAATTTTTT 
CATCAATTTA TTTCTCTCTC CACAAGTGGA GGAAAGTTTC AGTTTTTAGC AGATTCAGAT 
TCTTGCCTCA CGCTGAGCTT GAACCATCCA CTGGCTCCAG TGAGACTTTT GGCAGTTAAC 
CATCTGAAAA ATATTATGGA AACATCAAAG GACAATATTG ATGCTTCTTT CATAAAAGAA 
GCTATTATAA TGCGTCTTGG TGATGATAAT TTAGATGTTG TTCTGTCAGC CATGAATGCT 
TTTGTGGTTT TCAAAAAAGA GTTTAGCCCA GAAGAAACAG TTTCAAGTCT TTTGAGACTT 
TTTCAAAGAG CAGAACTTTC ACAGGATGGG GCATGGTACA AAATACTTGA ATTAGCAGCT 
GACACTCTAA TTAAAGAAGA GATTCTGAAT GGAAATGATG AGTTACTAAA TCAAGTGGTC 
ATACATTTGC TGCCATTTAT GGTTGTTACC AATGACAATT CAAAATCCCA AGAAATAAAA 
ATGGCTACTT ATCTGTCAAA ATCAGGAATT TGCTCTTTGC ACCCTATACT GAAAGGATGG 
AAAGAAGCTC TTGAAAAAGT GATTAAAACC ACAAAGCCAA CAGAATTGAT TGGTGTAGCA 
AATCAGAAAA TGGTTCAGTT GTTGGCTGAC AATATAAATT CAGGGAATCC TTCTTCAATG 
TTAAAGATGG TGGAAGATTT GATAGCTGTT GGTGAAAAGG AAAGCTACAC ATTGAAGCAG 
AAAGTAACTT CCCAAGTTGT CATTTCAGTT CTTGTCTCTT GCTGCTCTTC ATTAAAGGAA 
ACTCACTTTC CATTTGCATT GAAAGTCTTC AATTTGCTGG AAAGGAAAAT AAGAGAAAAT 
GAAGTTATTA CTTCGTTGGA AATCTCTTTT GAATGTCATT CTGAACTGTC AGACAAAGGA 
ATACCAGTGG AGATATGGAT TCAGTATGTA GAAAAGCTCA ACAGAGGGCA GATGATTGCT 
GTGGAAGATG CTGTTATGCT TGCATTTTCA TTGAAAAACT TTATTCAAGT CTTGAAGCCT 
CCTAAATCTT TTCTTAAAGA TGATAAGTGG TGGAATCCTG AACATATGAA AGAAGACAGC 
CGAGACTATC TGCACTTATT GATCCAGATA TTTGAGGTTA TTATTGCTGG TGCTGATGCC 
CTTTGCTTCA GAGTTCTGAT GAAACTTTTC TTAAAGGTAC ATTTAGAAGA TGTTTTTGAG 
TTATTCAAGT TCTTCTCACT TTTATGGACC TATAATTATA GCCTTTCAAA TCCACTTAAC 
TATAATGTGA AAACAATACT ACAGACTCAA GCTCTTTACA TAGGACGTGC AATGCTGGCA 
GGTCAGGCTG CACAGAAGAA AAAATACCTG GCATCAACAT CTTCTCCAGT GGTGATATGT 
TTACTCATAA ATTTGGGAAG TCCTATAAGA GAAGTACGAA GAGCTGCCAT TCTCTGCCTC 
CAGACCTTAC GTGAAGTAGA GTCTCCACTT TATCCTGTAA TACAACACCT AGTCCTAAAA 
GAAGAGGAGA TTATTTCAGA TGCTACATAT GTAGCACAGG ATTTGAATAC TTTATTTGAA 
GATCTACAAA CAGAAAAAAA GCCAAAATCT CACCAGACAT TAACTGAAGC CTTAAAACAT 
TTGCTTTATT GTGTACATAG CTGTCCATCC TATATTGCAA AAAACTTGAT GAAAATTCTT 
CAGGAAGTCA ATAGTGAGAC GGTGCTTTCT CAGCTGTTGC CCACAATTGA TCAGTTGCTA 
GAGAAAGTCC TAAAGGAACC CCCTACAGTC CTGAAGGATG AAGCTGTCAT TTTGCACCTT 
GCCCTTGGGA AATACAATGA GCATTCAGCT TCTCTCTTGC ACAAGGACCC ACAGAGTTTA 
GATATATTCC TCAAAGCTGT GCATACCACC AAGGAAGTCT TCGAAGGGAT GCCTACATTC 
CAGATCCTTG CCCTTGGGAA GATTACAAAA CCATTCTTTG CTGCTATATC AGATGACAAA 
GTTCAACAGA AGCTTCTGGG AATGCTGTTT GATTTACTTT TGAATTGCAA AAATACACTC 
TGTGCTCAGA CTGTTAGCAG TGTTTTTAAA ACAATTTCTG TTGATGCTGA ACAAATCCGA 
TTAGAACTAG AACCACCAGA GAAAAACAAG TCCTTGGGTA CAATTCAGCA AACAAGAAGA 
CAGAAAATGC AACAGAAAAA ACTACAAGAT ACAGAACCTG CACAAGATGT TGGCAGTGCT 
TACTGGCAGA GAGTAACCCT CATCTTAGAA TTACTGCAGC ACAAAAAGAA ACTCAAAAGT 
CCTCAGTTAT TAGTGCCATG TCTTTTTAAT TTGCTCTCCA GGTGTCTAGA AACTTTGCCA 
CCGGAGCAGG GAAATCTGGA ATATACCAAA CAGCTAATTC TTAGCTGTCT ACTTAATATC 
TGCCAGAAAC TATCCCCAGA TGGTTCTAAG ATCCCTAAAG GTATTCTTGA TGAAGAAAAA 
TTCAATGTTG AATTGTTAGT TCAGTGTGTG CGGGTTTCAG AGATGCCTCA GACACATCAC 
CATGCCCTCT TGCTTTTGGG TGCAGTTGCT GGAATATTTC CTGATAAAGT TTTGCACAAC 
ATTATGCCCA TCTTTACATT CATGGGAGCC AATGTTATGC GCCTTGATGA CACTTACAGT 
TTTCAAGTTA TCAACAAGAC AGTGAAAATG GTCATTCCTG CTCTTATCCA GGCAGACAGT 
GGTAAGTCTG CAGATGTCAC AAGGAACATA GAAGAAGTTG TGGTTAAAAT TATAAATGTG 
TTTGTGGATG CTCTACCTCA TGTTCCCGAA CATAGACGCC TGCCCATCCT TGTACAACTT 
GTTGATACTC TTGGTGCAGA GAAATTCCTC TGGATTCTCC TGCTGTTGCT TTTTGAACAG 
TATGTCACTA AAACAGTGTT GGTTGCTGCT CATGGAGAAA AGGATGCTAT CTTAGAGACT 
GACACTGAAT TTTGGATATC AGTCTCTTGT GAATTTACTG TCCAACAGCA GCTCCAAAGT 
TTAATGAATA TACTCAGATA CTTAACCAAA CTTCCAGAGA AAAAAGAAGG AGAAGAACCT 
CCCAAAACAG GATCTCACAA CTCCAAGACA CTGGAACAGA TGGTACAGGT TTTCAATGTG 
GACACTCATA CCAGCAAGCA GCTCCGACAT TTTACATTTT TGTCTGTGTC CTTTATGTCT 
CAACTCTTGG CTTCCCATAA TTTTATCAAA AAGGTCATGG AAAATGGTGG TACTGTGGCT 
TTACAAGGCC TTGAACAGAG ATTACTTGAA ACTGTTCTTG GTTATATAAA TTCTGTGGCA 
CAGTCAGTGG AGGAAAATGC AGACAAATTA ACTGCAAAGT TTTGGCGTGC TCTCTTGAGT 
AAAGCTTATG ATTTGCTTGA TAAAGTCAAT TCCTTATTGC CCACAGAAAC TTTTGTTCCT 
GTGATAAGAG GATTGCTGAC TAACCAACTG CCTTCTGTCC GTCGTAAAGC AATGGACCTC 
TTAAACAATA AATTGCATCA GTATGCAAAG TGGAAAAAGG GACAGGTGAC CCTTTTCTTG 
AAACTGGTTC CAGATCTCTT GGATATTGCA CAGCATAAAA TAAAGGAGAA AGAAGAAGAA 
CAGGCCATCA ACAGACAGAC TGCCTTGTAC AGTTTGAAAT TACTCTGCAA AAATTTTGGC 
ACAGAAAATT CAGAGGTTTT TATCCCTGTA TTGAGTGCAG CCATTAACCT GATCAATCCA 
GAGGTGAAGG ATGAGAAGAA CGTATTGGGA AGTGCTTTGT TGTGTATAGC TGAGGTCACC 
TACGTACTGG GAGCATTAGC AATACCTCAT CTTCCCAGAC TTATGCCTGC ATTATTGACT 
ACCCTGGAGA ATACGAACGA GCTGATCTCA AGTGAGGTTT ACTTGCTTAG TGCATTGGCA 
GCCCTTCAGA AAGTTGTTGA AACTCTTCCA CATTTTCTCA GCCCATACCT ACAGGGTATT 
TTGATACAGG CAATTCGTTG GGAGACAGTT GTGAAGGAGA TGGGTCCTAC TTCCCAGGCA 
ACTCTCCGAG TGACATCCCT GAAAGCAACA CTGGCTACTA CACTTTCTTC AAGAGTTTTA 
CTGTCAGCAC TCAACACAGT TTACAAGCAA ATTGGAAAGA ACTGGAAGAA TCGAATAGGT 
CCATTTATGA GCCTCCTTCA AGAGCACATT GCAGTAATGG AGAAGGAAGA CCTCAACTCT 
CATCAGTCTC AGTTAACAGC ATTTTTCTTG GAAGCCTTGA ACTTCAGATC ATTACATTCA 
GAGGATAACC TAGAAGAAGT TGGTCAAACT GAAGGTTGTA TAGTTAGCTG TCTAATGAGC 
ATGGTTATGA AACTTTCTGA AGTTACTTTC AGACCTCTCT TCTTTAAGCT ATTTGATTGG 
GCCAGAACAG AAGATGCTCC AAAGGACAGG CTGCTGACAT TCTGCAACTT GGCAGATTGT 
ATTGCTGAAA AATTGAAAGG CCTTTTTACC CTATTTGCTG GTCATTTAGT GAAACCTTTT 
GCTGACACTT TGAACCAAAT CAACATCTCT AAAACAGACG AAGCCTTTTT TGACTCTGGA 
AATGAACCTG AAAAGAGCTG TTTGTTACTG CAGTCTATAT TGGATTGTCT GCACAAAATC 
TTTCTTTTTG ACACTCAGCA TTTTGTAAGC AAAGAGAGAG CTGAAGCACT GATGATGCCT 
CTGGTGGATC AGCTGGAAAA CATGCTTGGA GGAGAAGAGA CATTTCAGGA AAGAGTGACG 
AAGCACCTAA TACCGTGTAT TGCTCAGTTT TCTGTAGCCA TGGCAGATGA TTCTCTTTGG 
AAACCATTGA ACTACCAAAT TCTCCTTAAG ATGCGGCATT CTTCACCTAA GGTTCGATTC 
GCTGCCTTGA TTACAGTTTT AGAGCTGGCT GAAAAATTAA AGGAGAACTA CATCGTGCTT 
CTCCCTGAAT CCATTCCTTT CTTAGCAGAG CTGATGGAAG ATGAATGTGA AGAAGTGGAA 
AATCAGTGCC AGAAGACTAT TCAACAACTG GAAGTCATCT TGGGAGAGCC ACTCCAGAGC 
TATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012403412.1
CDS	90..6518
Translation	MTSLSQQLQRLALPQNDPSLLARHEVASLLFDCKEAASIDRDTIFAIGCTGLEELIGIDPSFQKFESSLFSQLAKSLERSVQTKAVNKQLDENISLFLIHLSPYFMLKPAQKCLEWLIHRFHIHLYNQDSLIGCVLPYHETKIFVRVIQLLKISSPTHKWNWLEPVKMSGVHLAKGTLVTHCYKDLGFMDFICSLVTKSVKVFSEYPGSSAQLRVLLTFYASTIVSALVSANDISDSLLAKLFPYIQKGLKSSLPDYRAATYMIICQVSVKVTMEDTFVHSLASQIGKTLTKIPSLIKNGLGCLIVLLQRQKVEGLGKKLFAHLCNVPDLVSLLHEISVTYDISPLLHYMLPHLVVTIVHHVTEEETEELDSHIYARLLEDILKDISLENNLDHLLASTLLEEYISYSSRKEIDSKEISLLNEQLLPLIRLLESKYPKTLDLVLEDHLKDVTDPQKQEFFHQFISLSTSGGKFQFLADSDSCLTLSLNHPLAPVRLLAVNHLKNIMETSKDNIDASFIKEAIIMRLGDDNLDVVLSAMNAFVVFKKEFSPEETVSSLLRLFQRAELSQDGAWYKILELAADTLIKEEILNGNDELLNQVVIHLLPFMVVTNDNSKSQEIKMATYLSKSGICSLHPILKGWKEALEKVIKTTKPTELIGVANQKMVQLLADNINSGNPSSMLKMVEDLIAVGEKESYTLKQKVTSQVVISVLVSCCSSLKETHFPFALKVFNLLERKIRENEVITSLEISFECHSELSDKGIPVEIWIQYVEKLNRGQMIAVEDAVMLAFSLKNFIQVLKPPKSFLKDDKWWNPEHMKEDSRDYLHLLIQIFEVIIAGADALCFRVLMKLFLKVHLEDVFELFKFFSLLWTYNYSLSNPLNYNVKTILQTQALYIGRAMLAGQAAQKKKYLASTSSPVVICLLINLGSPIREVRRAAILCLQTLREVESPLYPVIQHLVLKEEEIISDATYVAQDLNTLFEDLQTEKKPKSHQTLTEALKHLLYCVHSCPSYIAKNLMKILQEVNSETVLSQLLPTIDQLLEKVLKEPPTVLKDEAVILHLALGKYNEHSASLLHKDPQSLDIFLKAVHTTKEVFEGMPTFQILALGKITKPFFAAISDDKVQQKLLGMLFDLLLNCKNTLCAQTVSSVFKTISVDAEQIRLELEPPEKNKSLGTIQQTRRQKMQQKKLQDTEPAQDVGSAYWQRVTLILELLQHKKKLKSPQLLVPCLFNLLSRCLETLPPEQGNLEYTKQLILSCLLNICQKLSPDGSKIPKGILDEEKFNVELLVQCVRVSEMPQTHHHALLLLGAVAGIFPDKVLHNIMPIFTFMGANVMRLDDTYSFQVINKTVKMVIPALIQADSGKSADVTRNIEEVVVKIINVFVDALPHVPEHRRLPILVQLVDTLGAEKFLWILLLLLFEQYVTKTVLVAAHGEKDAILETDTEFWISVSCEFTVQQQLQSLMNILRYLTKLPEKKEGEEPPKTGSHNSKTLEQMVQVFNVDTHTSKQLRHFTFLSVSFMSQLLASHNFIKKVMENGGTVALQGLEQRLLETVLGYINSVAQSVEENADKLTAKFWRALLSKAYDLLDKVNSLLPTETFVPVIRGLLTNQLPSVRRKAMDLLNNKLHQYAKWKKGQVTLFLKLVPDLLDIAQHKIKEKEEEQAINRQTALYSLKLLCKNFGTENSEVFIPVLSAAINLINPEVKDEKNVLGSALLCIAEVTYVLGALAIPHLPRLMPALLTTLENTNELISSEVYLLSALAALQKVVETLPHFLSPYLQGILIQAIRWETVVKEMGPTSQATLRVTSLKATLATTLSSRVLLSALNTVYKQIGKNWKNRIGPFMSLLQEHIAVMEKEDLNSHQSQLTAFFLEALNFRSLHSEDNLEEVGQTEGCIVSCLMSMVMKLSEVTFRPLFFKLFDWARTEDAPKDRLLTFCNLADCIAEKLKGLFTLFAGHLVKPFADTLNQINISKTDEAFFDSGNEPEKSCLLLQSILDCLHKIFLFDTQHFVSKERAEALMMPLVDQLENMLGGEETFQERVTKHLIPCIAQFSVAMADDSLWKPLNYQILLKMRHSSPKVRFAALITVLELAEKLKENYIVLLPESIPFLAELMEDECEEVENQCQKTIQQLEVILGEPLQSYF

Target ORF information:

RefSeq Version	XM_012547958.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012547958.2

ORF Insert Sequence:

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAAC TGAGGAACTG GACAGTCATA TATATGCTAG ACTCTTAGAA 
GATATACTTA AAGATATATC ATTGGAAAAC AACTTAGACC ACTTGTTGGC TAGCACTCTT 
CTTGAAGAAT ATATTTCATA TAGTTCAAGG AAAGAAATAG ATTCTAAGGA GATATCACTA 
CTCAATGAAC AACTCCTTCC TCTCATTAGA CTTTTGGAAA GCAAATACCC TAAGACATTA 
GATTTGGTAT TGGAAGACCA TCTGAAGGAT GTTACAGATC CCCAAAAGCA AGAATTTTTT 
CATCAATTTA TTTCTCTCTC CACAAGTGGA GGAAAGTTTC AGTTTTTAGC AGATTCAGAT 
TCTTGCCTCA CGCTGAGCTT GAACCATCCA CTGGCTCCAG TGAGACTTTT GGCAGTTAAC 
CATCTGAAAA ATATTATGGA AACATCAAAG GACAATATTG ATGCTTCTTT CATAAAAGAA 
GCTATTATAA TGCGTCTTGG TGATGATAAT TTAGATGTTG TTCTGTCAGC CATGAATGCT 
TTTGTGGTTT TCAAAAAAGA GTTTAGCCCA GAAGAAACAG TTTCAAGTCT TTTGAGACTT 
TTTCAAAGAG CAGAACTTTC ACAGGATGGG GCATGGTACA AAATACTTGA ATTAGCAGCT 
GACACTCTAA TTAAAGAAGA GATTCTGAAT GGAAATGATG AGTTACTAAA TCAAGTGGTC 
ATACATTTGC TGCCATTTAT GGTTGTTACC AATGACAATT CAAAATCCCA AGAAATAAAA 
ATGGCTACTT ATCTGTCAAA ATCAGGAATT TGCTCTTTGC ACCCTATACT GAAAGGATGG 
AAAGAAGCTC TTGAAAAAGT GATTAAAACC ACAAAGCCAA CAGAATTGAT TGGTGTAGCA 
AATCAGAAAA TGGTTCAGTT GTTGGCTGAC AATATAAATT CAGGGAATCC TTCTTCAATG 
TTAAAGATGG TGGAAGATTT GATAGCTGTT GGTGAAAAGG AAAGCTACAC ATTGAAGCAG 
AAAGTAACTT CCCAAGTTGT CATTTCAGTT CTTGTCTCTT GCTGCTCTTC ATTAAAGGAA 
ACTCACTTTC CATTTGCATT GAAAGTCTTC AATTTGCTGG AAAGGAAAAT AAGAGAAAAT 
GAAGTTATTA CTTCGTTGGA AATCTCTTTT GAATGTCATT CTGAACTGTC AGACAAAGGA 
ATACCAGTGG AGATATGGAT TCAGTATGTA GAAAAGCTCA ACAGAGGGCA GATGATTGCT 
GTGGAAGATG CTGTTATGCT TGCATTTTCA TTGAAAAACT TTATTCAAGT CTTGAAGCCT 
CCTAAATCTT TTCTTAAAGA TGATAAGTGG TGGAATCCTG AACATATGAA AGAAGACAGC 
CGAGACTATC TGCACTTATT GATCCAGATA TTTGAGGTTA TTATTGCTGG TGCTGATGCC 
CTTTGCTTCA GAGTTCTGAT GAAACTTTTC TTAAAGGTAC ATTTAGAAGA TGTTTTTGAG 
TTATTCAAGT TCTTCTCACT TTTATGGACC TATAATTATA GCCTTTCAAA TCCACTTAAC 
TATAATGTGA AAACAATACT ACAGACTCAA GCTCTTTACA TAGGACGTGC AATGCTGGCA 
GGTCAGGCTG CACAGAAGAA AAAATACCTG GCATCAACAT CTTCTCCAGT GGTGATATGT 
TTACTCATAA ATTTGGGAAG TCCTATAAGA GAAGTACGAA GAGCTGCCAT TCTCTGCCTC 
CAGACCTTAC GTGAAGTAGA GTCTCCACTT TATCCTGTAA TACAACACCT AGTCCTAAAA 
GAAGAGGAGA TTATTTCAGA TGCTACATAT GTAGCACAGG ATTTGAATAC TTTATTTGAA 
GATCTACAAA CAGAAAAAAA GCCAAAATCT CACCAGACAT TAACTGAAGC CTTAAAACAT 
TTGCTTTATT GTGTACATAG CTGTCCATCC TATATTGCAA AAAACTTGAT GAAAATTCTT 
CAGGAAGTCA ATAGTGAGAC GGTGCTTTCT CAGCTGTTGC CCACAATTGA TCAGTTGCTA 
GAGAAAGTCC TAAAGGAACC CCCTACAGTC CTGAAGGATG AAGCTGTCAT TTTGCACCTT 
GCCCTTGGGA AATACAATGA GCATTCAGCT TCTCTCTTGC ACAAGGACCC ACAGAGTTTA 
GATATATTCC TCAAAGCTGT GCATACCACC AAGGAAGTCT TCGAAGGGAT GCCTACATTC 
CAGATCCTTG CCCTTGGGAA GATTACAAAA CCATTCTTTG CTGCTATATC AGATGACAAA 
GTTCAACAGA AGCTTCTGGG AATGCTGTTT GATTTACTTT TGAATTGCAA AAATACACTC 
TGTGCTCAGA CTGTTAGCAG TGTTTTTAAA ACAATTTCTG TTGATGCTGA ACAAATCCGA 
TTAGAACTAG AACCACCAGA GAAAAACAAG TCCTTGGGTA CAATTCAGCA AACAAGAAGA 
CAGAAAATGC AACAGAAAAA ACTACAAGAT ACAGAACCTG CACAAGATGT TGGCAGTGCT 
TACTGGCAGA GAGTAACCCT CATCTTAGAA TTACTGCAGC ACAAAAAGAA ACTCAAAAGT 
CCTCAGTTAT TAGTGCCATG TCTTTTTAAT TTGCTCTCCA GGTGTCTAGA AACTTTGCCA 
CCGGAGCAGG GAAATCTGGA ATATACCAAA CAGCTAATTC TTAGCTGTCT ACTTAATATC 
TGCCAGAAAC TATCCCCAGA TGGTTCTAAG ATCCCTAAAG GTATTCTTGA TGAAGAAAAA 
TTCAATGTTG AATTGTTAGT TCAGTGTGTG CGGGTTTCAG AGATGCCTCA GACACATCAC 
CATGCCCTCT TGCTTTTGGG TGCAGTTGCT GGAATATTTC CTGATAAAGT TTTGCACAAC 
ATTATGCCCA TCTTTACATT CATGGGAGCC AATGTTATGC GCCTTGATGA CACTTACAGT 
TTTCAAGTTA TCAACAAGAC AGTGAAAATG GTCATTCCTG CTCTTATCCA GGCAGACAGT 
GGTAAGTCTG CAGATGTCAC AAGGAACATA GAAGAAGTTG TGGTTAAAAT TATAAATGTG 
TTTGTGGATG CTCTACCTCA TGTTCCCGAA CATAGACGCC TGCCCATCCT TGTACAACTT 
GTTGATACTC TTGGTGCAGA GAAATTCCTC TGGATTCTCC TGCTGTTGCT TTTTGAACAG 
TATGTCACTA AAACAGTGTT GGTTGCTGCT CATGGAGAAA AGGATGCTAT CTTAGAGACT 
GACACTGAAT TTTGGATATC AGTCTCTTGT GAATTTACTG TCCAACAGCA GCTCCAAAGT 
TTAATGAATA TACTCAGATA CTTAACCAAA CTTCCAGAGA AAAAAGAAGG AGAAGAACCT 
CCCAAAACAG GATCTCACAA CTCCAAGACA CTGGAACAGA TGGTACAGGT TTTCAATGTG 
GACACTCATA CCAGCAAGCA GCTCCGACAT TTTACATTTT TGTCTGTGTC CTTTATGTCT 
CAACTCTTGG CTTCCCATAA TTTTATCAAA AAGGTCATGG AAAATGGTGG TACTGTGGCT 
TTACAAGGCC TTGAACAGAG ATTACTTGAA ACTGTTCTTG GTTATATAAA TTCTGTGGCA 
CAGTCAGTGG AGGAAAATGC AGACAAATTA ACTGCAAAGT TTTGGCGTGC TCTCTTGAGT 
AAAGCTTATG ATTTGCTTGA TAAAGTCAAT TCCTTATTGC CCACAGAAAC TTTTGTTCCT 
GTGATAAGAG GATTGCTGAC TAACCAACTG CCTTCTGTCC GTCGTAAAGC AATGGACCTC 
TTAAACAATA AATTGCATCA GTATGCAAAG TGGAAAAAGG GACAGGTGAC CCTTTTCTTG 
AAACTGGTTC CAGATCTCTT GGATATTGCA CAGCATAAAA TAAAGGAGAA AGAAGAAGAA 
CAGGCCATCA ACAGACAGAC TGCCTTGTAC AGTTTGAAAT TACTCTGCAA AAATTTTGGC 
ACAGAAAATT CAGAGGTTTT TATCCCTGTA TTGAGTGCAG CCATTAACCT GATCAATCCA 
GAGGTGAAGG ATGAGAAGAA CGTATTGGGA AGTGCTTTGT TGTGTATAGC TGAGGTCACC 
TACGTACTGG GAGCATTAGC AATACCTCAT CTTCCCAGAC TTATGCCTGC ATTATTGACT 
ACCCTGGAGA ATACGAACGA GCTGATCTCA AGTGAGGTTT ACTTGCTTAG TGCATTGGCA 
GCCCTTCAGA AAGTTGTTGA AACTCTTCCA CATTTTCTCA GCCCATACCT ACAGGGTATT 
TTGATACAGG CAATTCGTTG GGAGACAGTT GTGAAGGAGA TGGGTCCTAC TTCCCAGGCA 
ACTCTCCGAG TGACATCCCT GAAAGCAACA CTGGCTACTA CACTTTCTTC AAGAGTTTTA 
CTGTCAGCAC TCAACACAGT TTACAAGCAA ATTGGAAAGA ACTGGAAGAA TCGAATAGGT 
CCATTTATGA GCCTCCTTCA AGAGCACATT GCAGTAATGG AGAAGGAAGA CCTCAACTCT 
CATCAGTCTC AGTTAACAGC ATTTTTCTTG GAAGCCTTGA ACTTCAGATC ATTACATTCA 
GAGGATAACC TAGAAGAAGT TGGTCAAACT GAAGGTTGTA TAGTTAGCTG TCTAATGAGC 
ATGGTTATGA AACTTTCTGA AGTTACTTTC AGACCTCTCT TCTTTAAGCT ATTTGATTGG 
GCCAGAACAG AAGATGCTCC AAAGGACAGG CTGCTGACAT TCTGCAACTT GGCAGATTGT 
ATTGCTGAAA AATTGAAAGG CCTTTTTACC CTATTTGCTG GTCATTTAGT GAAACCTTTT 
GCTGACACTT TGAACCAAAT CAACATCTCT AAAACAGACG AAGCCTTTTT TGACTCTGGA 
AATGAACCTG AAAAGAGCTG TTTGTTACTG CAGTCTATAT TGGATTGTCT GCACAAAATC 
TTTCTTTTTG ACACTCAGCA TTTTGTAAGC AAAGAGAGAG CTGAAGCACT GATGATGCCT 
CTGGTGGATC AGCTGGAAAA CATGCTTGGA GGAGAAGAGA CATTTCAGGA AAGAGTGACG 
AAGCACCTAA TACCGTGTAT TGCTCAGTTT TCTGTAGCCA TGGCAGATGA TTCTCTTTGG 
AAACCATTGA ACTACCAAAT TCTCCTTAAG ATGCGGCATT CTTCACCTAA GGTTCGATTC 
GCTGCCTTGA TTACAGTTTT AGAGCTGGCT GAAAAATTAA AGGAGAACTA CATCGTGCTT 
CTCCCTGAAT CCATTCCTTT CTTAGCAGAG CTGATGGAAG ATGAATGTGA AGAAGTGGAA 
AATCAGTGCC AGAAGACTAT TCAACAACTG GAAGTCATCT TGGGAGAGCC ACTCCAGAGC 
TATTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR1 ( XM_023502057.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023357825.1 Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X1, mRNA.

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CloneID	OSa154067
Clone ID Related Accession (Same CDS sequence)	XM_023502057.2 , XM_023502057.1
Accession Version	XM_023502057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023502057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAGA AACTGAGGAA CTGGACAGTC ATATATATGC TAGACTCTTA 
GAAGATATAC TTAAAGATAT ATCATTGGAA AACAACTTAG ACCACTTGTT GGCTAGCACT 
CTTCTTGAAG AATATATTTC ATATAGTTCA AGGAAAGAAA TAGATTCTAA GGAGATATCA 
CTACTCAATG AACAACTCCT TCCTCTCATT AGACTTTTGG AAAGCAAATA CCCTAAGACA 
TTAGATTTGG TATTGGAAGA CCATCTGAAG GATGTTACAG ATCCCCAAAA GCAAGAATTT 
TTTCATCAAT TTATTTCTCT CTCCACAAGT GGAGGAAAGT TTCAGTTTTT AGCAGATTCA 
GATTCTTGCC TCACGCTGAG CTTGAACCAT CCACTGGCTC CAGTGAGACT TTTGGCAGTT 
AACCATCTGA AAAATATTAT GGAAACATCA AAGGACAATA TTGATGCTTC TTTCATAAAA 
GAAGCTATTA TAATGCGTCT TGGTGATGAT AATTTAGATG TTGTTCTGTC AGCCATGAAT 
GCTTTTGTGG TTTTCAAAAA AGAGTTTAGC CCAGAAGAAA CAGTTTCAAG TCTTTTGAGA 
CTTTTTCAAA GAGCAGAACT TTCACAGGAT GGGGCATGGT ACAAAATACT TGAATTAGCA 
GCTGACACTC TAATTAAAGA AGAGATTCTG AATGGAAATG ATGAGTTACT AAATCAAGTG 
GTCATACATT TGCTGCCATT TATGGTTGTT ACCAATGACA ATTCAAAATC CCAAGAAATA 
AAAATGGCTA CTTATCTGTC AAAATCAGGA ATTTGCTCTT TGCACCCTAT ACTGAAAGGA 
TGGAAAGAAG CTCTTGAAAA AGTGATTAAA ACCACAAAGC CAACAGAATT GATTGGTGTA 
GCAAATCAGA AAATGGTTCA GTTGTTGGCT GACAATATAA ATTCAGGGAA TCCTTCTTCA 
ATGTTAAAGA TGGTGGAAGA TTTGATAGCT GTTGGTGAAA AGGAAAGCTA CACATTGAAG 
CAGAAAGTAA CTTCCCAAGT TGTCATTTCA GTTCTTGTCT CTTGCTGCTC TTCATTAAAG 
GAAACTCACT TTCCATTTGC ATTGAAAGTC TTCAATTTGC TGGAAAGGAA AATAAGAGAA 
AATGAAGTTA TTACTTCGTT GGAAATCTCT TTTGAATGTC ATTCTGAACT GTCAGACAAA 
GGAATACCAG TGGAGATATG GATTCAGTAT GTAGAAAAGC TCAACAGAGG GCAGATGATT 
GCTGTGGAAG ATGCTGTTAT GCTTGCATTT TCATTGAAAA ACTTTATTCA AGTCTTGAAG 
CCTCCTAAAT CTTTTCTTAA AGATGATAAG TGGTGGAATC CTGAACATAT GAAAGAAGAC 
AGCCGAGACT ATCTGCACTT ATTGATCCAG ATATTTGAGG TTATTATTGC TGGTGCTGAT 
GCCCTTTGCT TCAGAGTTCT GATGAAACTT TTCTTAAAGG TACATTTAGA AGATGTTTTT 
GAGTTATTCA AGTTCTTCTC ACTTTTATGG ACCTATAATT ATAGCCTTTC AAATCCACTT 
AACTATAATG TGAAAACAAT ACTACAGACT CAAGCTCTTT ACATAGGACG TGCAATGCTG 
GCAGGTCAGG CTGCACAGAA GAAAAAATAC CTGGCATCAA CATCTTCTCC AGTGGTGATA 
TGTTTACTCA TAAATTTGGG AAGTCCTATA AGAGAAGTAC GAAGAGCTGC CATTCTCTGC 
CTCCAGACCT TACGTGAAGT AGAGTCTCCA CTTTATCCTG TAATACAACA CCTAGTCCTA 
AAAGAAGAGG AGATTATTTC AGATGCTACA TATGTAGCAC AGGATTTGAA TACTTTATTT 
GAAGATCTAC AAACAGAAAA AAAGCCAAAA TCTCACCAGA CATTAACTGA AGCCTTAAAA 
CATTTGCTTT ATTGTGTACA TAGCTGTCCA TCCTATATTG CAAAAAACTT GATGAAAATT 
CTTCAGGAAG TCAATAGTGA GACGGTGCTT TCTCAGCTGT TGCCCACAAT TGATCAGTTG 
CTAGAGAAAG TCCTAAAGGA ACCCCCTACA GTCCTGAAGG ATGAAGCTGT CATTTTGCAC 
CTTGCCCTTG GGAAATACAA TGAGCATTCA GCTTCTCTCT TGCACAAGGA CCCACAGAGT 
TTAGATATAT TCCTCAAAGC TGTGCATACC ACCAAGGAAG TCTTCGAAGG GATGCCTACA 
TTCCAGATCC TTGCCCTTGG GAAGATTACA AAACCATTCT TTGCTGCTAT ATCAGATGAC 
AAAGTTCAAC AGAAGCTTCT GGGAATGCTG TTTGATTTAC TTTTGAATTG CAAAAATACA 
CTCTGTGCTC AGACTGTTAG CAGTGTTTTT AAAACAATTT CTGTTGATGC TGAACAAATC 
CGATTAGAAC TAGAACCACC AGAGAAAAAC AAGTCCTTGG GTACAATTCA GCAAACAAGA 
AGACAGAAAA TGCAACAGAA AAAACTACAA GATACAGAAC CTGCACAAGA TGTTGGCAGT 
GCTTACTGGC AGAGAGTAAC CCTCATCTTA GAATTACTGC AGCACAAAAA GAAACTCAAA 
AGTCCTCAGT TATTAGTGCC ATGTCTTTTT AATTTGCTCT CCAGGTGTCT AGAAACTTTG 
CCACCGGAGC AGGGAAATCT GGAATATACC AAACAGCTAA TTCTTAGCTG TCTACTTAAT 
ATCTGCCAGA AACTATCCCC AGATGGTTCT AAGATCCCTA AAGGTATTCT TGATGAAGAA 
AAATTCAATG TTGAATTGTT AGTTCAGTGT GTGCGGGTTT CAGAGATGCC TCAGACACAT 
CACCATGCCC TCTTGCTTTT GGGTGCAGTT GCTGGAATAT TTCCTGATAA AGTTTTGCAC 
AACATTATGC CCATCTTTAC ATTCATGGGA GCCAATGTTA TGCGCCTTGA TGACACTTAC 
AGTTTTCAAG TTATCAACAA GACAGTGAAA ATGGTCATTC CTGCTCTTAT CCAGGCAGAC 
AGTGGTAAGT CTGCAGATGT CACAAGGAAC ATAGAAGAAG TTGTGGTTAA AATTATAAAT 
GTGTTTGTGG ATGCTCTACC TCATGTTCCC GAACATAGAC GCCTGCCCAT CCTTGTACAA 
CTTGTTGATA CTCTTGGTGC AGAGAAATTC CTCTGGATTC TCCTGCTGTT GCTTTTTGAA 
CAGTATGTCA CTAAAACAGT GTTGGTTGCT GCTCATGGAG AAAAGGATGC TATCTTAGAG 
ACTGACACTG AATTTTGGAT ATCAGTCTCT TGTGAATTTA CTGTCCAACA GCAGCTCCAA 
AGTTTAATGA ATATACTCAG ATACTTAACC AAACTTCCAG AGAAAAAAGA AGGAGAAGAA 
CCTCCCAAAA CAGGATCTCA CAACTCCAAG ACACTGGAAC AGATGGTACA GGTTTTCAAT 
GTGGACACTC ATACCAGCAA GCAGCTCCGA CATTTTACAT TTTTGTCTGT GTCCTTTATG 
TCTCAACTCT TGGCTTCCCA TAATTTTATC AAAAAGGTCA TGGAAAATGG TGGTACTGTG 
GCTTTACAAG GCCTTGAACA GAGATTACTT GAAACTGTTC TTGGTTATAT AAATTCTGTG 
GCACAGTCAG TGGAGGAAAA TGCAGACAAA TTAACTGCAA AGTTTTGGCG TGCTCTCTTG 
AGTAAAGCTT ATGATTTGCT TGATAAAGTC AATTCCTTAT TGCCCACAGA AACTTTTGTT 
CCTGTGATAA GAGGATTGCT GACTAACCAA CTGCCTTCTG TCCGTCGTAA AGCAATGGAC 
CTCTTAAACA ATAAATTGCA TCAGTATGCA AAGTGGAAAA AGGGACAGGT GACCCTTTTC 
TTGAAACTGG TTCCAGATCT CTTGGATATT GCACAGCATA AAATAAAGGA GAAAGAAGAA 
GAACAGGCCA TCAACAGACA GACTGCCTTG TACAGTTTGA AATTACTCTG CAAAAATTTT 
GGCACAGAAA ATTCAGAGGT TTTTATCCCT GTATTGAGTG CAGCCATTAA CCTGATCAAT 
CCAGAGGTGA AGGATGAGAA GAACGTATTG GGAAGTGCTT TGTTGTGTAT AGCTGAGGTC 
ACCTACGTAC TGGGAGCATT AGCAATACCT CATCTTCCCA GACTTATGCC TGCATTATTG 
ACTACCCTGG AGAATACGAA CGAGCTGATC TCAAGTGAGG TTTACTTGCT TAGTGCATTG 
GCAGCCCTTC AGAAAGTTGT TGAAACTCTT CCACATTTTC TCAGCCCATA CCTACAGGGT 
ATTTTGATAC AGGCAATTCG TTGGGAGACA GTTGTGAAGG AGATGGGTCC TACTTCCCAG 
GCAACTCTCC GAGTGACATC CCTGAAAGCA ACACTGGCTA CTACACTTTC TTCAAGAGTT 
TTACTGTCAG CACTCAACAC AGTTTACAAG CAAATTGGAA AGAACTGGAA GAATCGAATA 
GGTCCATTTA TGAGCCTCCT TCAAGAGCAC ATTGCAGTAA TGGAGAAGGA AGACCTCAAC 
TCTCATCAGT CTCAGTTAAC AGCATTTTTC TTGGAAGCCT TGAACTTCAG ATCATTACAT 
TCAGAGGATA ACCTAGAAGA AGTTGGTCAA ACTGAAGGTT GTATAGTTAG CTGTCTAATG 
AGCATGGTTA TGAAACTTTC TGAAGTTACT TTCAGACCTC TCTTCTTTAA GCTATTTGAT 
TGGGCCAGAA CAGAAGATGC TCCAAAGGAC AGGCTGCTGA CATTCTGCAA CTTGGCAGAT 
TGTATTGCTG AAAAATTGAA AGGCCTTTTT ACCCTATTTG CTGGTCATTT AGTGAAACCT 
TTTGCTGACA CTTTGAACCA AATCAACATC TCTAAAACAG ACGAAGCCTT TTTTGACTCT 
GGAAATGAAC CTGAAAAGAG CTGTTTGTTA CTGCAGTCTA TATTGGATTG TCTGCACAAA 
ATCTTTCTTT TTGACACTCA GCATTTTGTA AGCAAAGAGA GAGCTGAAGC ACTGATGATG 
CCTCTGGTGG ATCAGCTGGA AAACATGCTT GGAGGAGAAG AGACATTTCA GGAAAGAGTG 
ACGAAGCACC TAATACCGTG TATTGCTCAG TTTTCTGTAG CCATGGCAGA TGATTCTCTT 
TGGAAACCAT TGAACTACCA AATTCTCCTT AAGATGCGGC ATTCTTCACC TAAGGTTCGA 
TTCGCTGCCT TGATTACAGT TTTAGAGCTG GCTGAAAAAT TAAAGGAGAA CTACATCGTG 
CTTCTCCCTG AATCCATTCC TTTCTTAGCA GAGCTGATGG AAGATGAATG TGAAGAAGTG 
GAAAATCAGT GCCAGAAGAC TATTCAACAA CTGGAAGTCA TCTTGGGAGA GCCACTCCAG 
AGCTATTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023357825.1
CDS	129..6560
Translation	MTSLSQQLQRLALPQNDPSLLARHEVASLLFDCKEAASIDRDTIFAIGCTGLEELIGIDPSFQKFESSLFSQLAKSLERSVQTKAVNKQLDENISLFLIHLSPYFMLKPAQKCLEWLIHRFHIHLYNQDSLIGCVLPYHETKIFVRVIQLLKISSPTHKWNWLEPVKMSGVHLAKGTLVTHCYKDLGFMDFICSLVTKSVKVFSEYPGSSAQLRVLLTFYASTIVSALVSANDISDSLLAKLFPYIQKGLKSSLPDYRAATYMIICQVSVKVTMEDTFVHSLASQIGKTLTKIPSLIKNGLGCLIVLLQRQKVEGLGKKLFAHLCNVPDLVSLLHEISVTYDISPLLHYMLPHLVVTIVHHVTEEEETEELDSHIYARLLEDILKDISLENNLDHLLASTLLEEYISYSSRKEIDSKEISLLNEQLLPLIRLLESKYPKTLDLVLEDHLKDVTDPQKQEFFHQFISLSTSGGKFQFLADSDSCLTLSLNHPLAPVRLLAVNHLKNIMETSKDNIDASFIKEAIIMRLGDDNLDVVLSAMNAFVVFKKEFSPEETVSSLLRLFQRAELSQDGAWYKILELAADTLIKEEILNGNDELLNQVVIHLLPFMVVTNDNSKSQEIKMATYLSKSGICSLHPILKGWKEALEKVIKTTKPTELIGVANQKMVQLLADNINSGNPSSMLKMVEDLIAVGEKESYTLKQKVTSQVVISVLVSCCSSLKETHFPFALKVFNLLERKIRENEVITSLEISFECHSELSDKGIPVEIWIQYVEKLNRGQMIAVEDAVMLAFSLKNFIQVLKPPKSFLKDDKWWNPEHMKEDSRDYLHLLIQIFEVIIAGADALCFRVLMKLFLKVHLEDVFELFKFFSLLWTYNYSLSNPLNYNVKTILQTQALYIGRAMLAGQAAQKKKYLASTSSPVVICLLINLGSPIREVRRAAILCLQTLREVESPLYPVIQHLVLKEEEIISDATYVAQDLNTLFEDLQTEKKPKSHQTLTEALKHLLYCVHSCPSYIAKNLMKILQEVNSETVLSQLLPTIDQLLEKVLKEPPTVLKDEAVILHLALGKYNEHSASLLHKDPQSLDIFLKAVHTTKEVFEGMPTFQILALGKITKPFFAAISDDKVQQKLLGMLFDLLLNCKNTLCAQTVSSVFKTISVDAEQIRLELEPPEKNKSLGTIQQTRRQKMQQKKLQDTEPAQDVGSAYWQRVTLILELLQHKKKLKSPQLLVPCLFNLLSRCLETLPPEQGNLEYTKQLILSCLLNICQKLSPDGSKIPKGILDEEKFNVELLVQCVRVSEMPQTHHHALLLLGAVAGIFPDKVLHNIMPIFTFMGANVMRLDDTYSFQVINKTVKMVIPALIQADSGKSADVTRNIEEVVVKIINVFVDALPHVPEHRRLPILVQLVDTLGAEKFLWILLLLLFEQYVTKTVLVAAHGEKDAILETDTEFWISVSCEFTVQQQLQSLMNILRYLTKLPEKKEGEEPPKTGSHNSKTLEQMVQVFNVDTHTSKQLRHFTFLSVSFMSQLLASHNFIKKVMENGGTVALQGLEQRLLETVLGYINSVAQSVEENADKLTAKFWRALLSKAYDLLDKVNSLLPTETFVPVIRGLLTNQLPSVRRKAMDLLNNKLHQYAKWKKGQVTLFLKLVPDLLDIAQHKIKEKEEEQAINRQTALYSLKLLCKNFGTENSEVFIPVLSAAINLINPEVKDEKNVLGSALLCIAEVTYVLGALAIPHLPRLMPALLTTLENTNELISSEVYLLSALAALQKVVETLPHFLSPYLQGILIQAIRWETVVKEMGPTSQATLRVTSLKATLATTLSSRVLLSALNTVYKQIGKNWKNRIGPFMSLLQEHIAVMEKEDLNSHQSQLTAFFLEALNFRSLHSEDNLEEVGQTEGCIVSCLMSMVMKLSEVTFRPLFFKLFDWARTEDAPKDRLLTFCNLADCIAEKLKGLFTLFAGHLVKPFADTLNQINISKTDEAFFDSGNEPEKSCLLLQSILDCLHKIFLFDTQHFVSKERAEALMMPLVDQLENMLGGEETFQERVTKHLIPCIAQFSVAMADDSLWKPLNYQILLKMRHSSPKVRFAALITVLELAEKLKENYIVLLPESIPFLAELMEDECEEVENQCQKTIQQLEVILGEPLQSYF

Target ORF information:

RefSeq Version	XM_023502057.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023502057.2

ORF Insert Sequence:

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAGA AACTGAGGAA CTGGACAGTC ATATATATGC TAGACTCTTA 
GAAGATATAC TTAAAGATAT ATCATTGGAA AACAACTTAG ACCACTTGTT GGCTAGCACT 
CTTCTTGAAG AATATATTTC ATATAGTTCA AGGAAAGAAA TAGATTCTAA GGAGATATCA 
CTACTCAATG AACAACTCCT TCCTCTCATT AGACTTTTGG AAAGCAAATA CCCTAAGACA 
TTAGATTTGG TATTGGAAGA CCATCTGAAG GATGTTACAG ATCCCCAAAA GCAAGAATTT 
TTTCATCAAT TTATTTCTCT CTCCACAAGT GGAGGAAAGT TTCAGTTTTT AGCAGATTCA 
GATTCTTGCC TCACGCTGAG CTTGAACCAT CCACTGGCTC CAGTGAGACT TTTGGCAGTT 
AACCATCTGA AAAATATTAT GGAAACATCA AAGGACAATA TTGATGCTTC TTTCATAAAA 
GAAGCTATTA TAATGCGTCT TGGTGATGAT AATTTAGATG TTGTTCTGTC AGCCATGAAT 
GCTTTTGTGG TTTTCAAAAA AGAGTTTAGC CCAGAAGAAA CAGTTTCAAG TCTTTTGAGA 
CTTTTTCAAA GAGCAGAACT TTCACAGGAT GGGGCATGGT ACAAAATACT TGAATTAGCA 
GCTGACACTC TAATTAAAGA AGAGATTCTG AATGGAAATG ATGAGTTACT AAATCAAGTG 
GTCATACATT TGCTGCCATT TATGGTTGTT ACCAATGACA ATTCAAAATC CCAAGAAATA 
AAAATGGCTA CTTATCTGTC AAAATCAGGA ATTTGCTCTT TGCACCCTAT ACTGAAAGGA 
TGGAAAGAAG CTCTTGAAAA AGTGATTAAA ACCACAAAGC CAACAGAATT GATTGGTGTA 
GCAAATCAGA AAATGGTTCA GTTGTTGGCT GACAATATAA ATTCAGGGAA TCCTTCTTCA 
ATGTTAAAGA TGGTGGAAGA TTTGATAGCT GTTGGTGAAA AGGAAAGCTA CACATTGAAG 
CAGAAAGTAA CTTCCCAAGT TGTCATTTCA GTTCTTGTCT CTTGCTGCTC TTCATTAAAG 
GAAACTCACT TTCCATTTGC ATTGAAAGTC TTCAATTTGC TGGAAAGGAA AATAAGAGAA 
AATGAAGTTA TTACTTCGTT GGAAATCTCT TTTGAATGTC ATTCTGAACT GTCAGACAAA 
GGAATACCAG TGGAGATATG GATTCAGTAT GTAGAAAAGC TCAACAGAGG GCAGATGATT 
GCTGTGGAAG ATGCTGTTAT GCTTGCATTT TCATTGAAAA ACTTTATTCA AGTCTTGAAG 
CCTCCTAAAT CTTTTCTTAA AGATGATAAG TGGTGGAATC CTGAACATAT GAAAGAAGAC 
AGCCGAGACT ATCTGCACTT ATTGATCCAG ATATTTGAGG TTATTATTGC TGGTGCTGAT 
GCCCTTTGCT TCAGAGTTCT GATGAAACTT TTCTTAAAGG TACATTTAGA AGATGTTTTT 
GAGTTATTCA AGTTCTTCTC ACTTTTATGG ACCTATAATT ATAGCCTTTC AAATCCACTT 
AACTATAATG TGAAAACAAT ACTACAGACT CAAGCTCTTT ACATAGGACG TGCAATGCTG 
GCAGGTCAGG CTGCACAGAA GAAAAAATAC CTGGCATCAA CATCTTCTCC AGTGGTGATA 
TGTTTACTCA TAAATTTGGG AAGTCCTATA AGAGAAGTAC GAAGAGCTGC CATTCTCTGC 
CTCCAGACCT TACGTGAAGT AGAGTCTCCA CTTTATCCTG TAATACAACA CCTAGTCCTA 
AAAGAAGAGG AGATTATTTC AGATGCTACA TATGTAGCAC AGGATTTGAA TACTTTATTT 
GAAGATCTAC AAACAGAAAA AAAGCCAAAA TCTCACCAGA CATTAACTGA AGCCTTAAAA 
CATTTGCTTT ATTGTGTACA TAGCTGTCCA TCCTATATTG CAAAAAACTT GATGAAAATT 
CTTCAGGAAG TCAATAGTGA GACGGTGCTT TCTCAGCTGT TGCCCACAAT TGATCAGTTG 
CTAGAGAAAG TCCTAAAGGA ACCCCCTACA GTCCTGAAGG ATGAAGCTGT CATTTTGCAC 
CTTGCCCTTG GGAAATACAA TGAGCATTCA GCTTCTCTCT TGCACAAGGA CCCACAGAGT 
TTAGATATAT TCCTCAAAGC TGTGCATACC ACCAAGGAAG TCTTCGAAGG GATGCCTACA 
TTCCAGATCC TTGCCCTTGG GAAGATTACA AAACCATTCT TTGCTGCTAT ATCAGATGAC 
AAAGTTCAAC AGAAGCTTCT GGGAATGCTG TTTGATTTAC TTTTGAATTG CAAAAATACA 
CTCTGTGCTC AGACTGTTAG CAGTGTTTTT AAAACAATTT CTGTTGATGC TGAACAAATC 
CGATTAGAAC TAGAACCACC AGAGAAAAAC AAGTCCTTGG GTACAATTCA GCAAACAAGA 
AGACAGAAAA TGCAACAGAA AAAACTACAA GATACAGAAC CTGCACAAGA TGTTGGCAGT 
GCTTACTGGC AGAGAGTAAC CCTCATCTTA GAATTACTGC AGCACAAAAA GAAACTCAAA 
AGTCCTCAGT TATTAGTGCC ATGTCTTTTT AATTTGCTCT CCAGGTGTCT AGAAACTTTG 
CCACCGGAGC AGGGAAATCT GGAATATACC AAACAGCTAA TTCTTAGCTG TCTACTTAAT 
ATCTGCCAGA AACTATCCCC AGATGGTTCT AAGATCCCTA AAGGTATTCT TGATGAAGAA 
AAATTCAATG TTGAATTGTT AGTTCAGTGT GTGCGGGTTT CAGAGATGCC TCAGACACAT 
CACCATGCCC TCTTGCTTTT GGGTGCAGTT GCTGGAATAT TTCCTGATAA AGTTTTGCAC 
AACATTATGC CCATCTTTAC ATTCATGGGA GCCAATGTTA TGCGCCTTGA TGACACTTAC 
AGTTTTCAAG TTATCAACAA GACAGTGAAA ATGGTCATTC CTGCTCTTAT CCAGGCAGAC 
AGTGGTAAGT CTGCAGATGT CACAAGGAAC ATAGAAGAAG TTGTGGTTAA AATTATAAAT 
GTGTTTGTGG ATGCTCTACC TCATGTTCCC GAACATAGAC GCCTGCCCAT CCTTGTACAA 
CTTGTTGATA CTCTTGGTGC AGAGAAATTC CTCTGGATTC TCCTGCTGTT GCTTTTTGAA 
CAGTATGTCA CTAAAACAGT GTTGGTTGCT GCTCATGGAG AAAAGGATGC TATCTTAGAG 
ACTGACACTG AATTTTGGAT ATCAGTCTCT TGTGAATTTA CTGTCCAACA GCAGCTCCAA 
AGTTTAATGA ATATACTCAG ATACTTAACC AAACTTCCAG AGAAAAAAGA AGGAGAAGAA 
CCTCCCAAAA CAGGATCTCA CAACTCCAAG ACACTGGAAC AGATGGTACA GGTTTTCAAT 
GTGGACACTC ATACCAGCAA GCAGCTCCGA CATTTTACAT TTTTGTCTGT GTCCTTTATG 
TCTCAACTCT TGGCTTCCCA TAATTTTATC AAAAAGGTCA TGGAAAATGG TGGTACTGTG 
GCTTTACAAG GCCTTGAACA GAGATTACTT GAAACTGTTC TTGGTTATAT AAATTCTGTG 
GCACAGTCAG TGGAGGAAAA TGCAGACAAA TTAACTGCAA AGTTTTGGCG TGCTCTCTTG 
AGTAAAGCTT ATGATTTGCT TGATAAAGTC AATTCCTTAT TGCCCACAGA AACTTTTGTT 
CCTGTGATAA GAGGATTGCT GACTAACCAA CTGCCTTCTG TCCGTCGTAA AGCAATGGAC 
CTCTTAAACA ATAAATTGCA TCAGTATGCA AAGTGGAAAA AGGGACAGGT GACCCTTTTC 
TTGAAACTGG TTCCAGATCT CTTGGATATT GCACAGCATA AAATAAAGGA GAAAGAAGAA 
GAACAGGCCA TCAACAGACA GACTGCCTTG TACAGTTTGA AATTACTCTG CAAAAATTTT 
GGCACAGAAA ATTCAGAGGT TTTTATCCCT GTATTGAGTG CAGCCATTAA CCTGATCAAT 
CCAGAGGTGA AGGATGAGAA GAACGTATTG GGAAGTGCTT TGTTGTGTAT AGCTGAGGTC 
ACCTACGTAC TGGGAGCATT AGCAATACCT CATCTTCCCA GACTTATGCC TGCATTATTG 
ACTACCCTGG AGAATACGAA CGAGCTGATC TCAAGTGAGG TTTACTTGCT TAGTGCATTG 
GCAGCCCTTC AGAAAGTTGT TGAAACTCTT CCACATTTTC TCAGCCCATA CCTACAGGGT 
ATTTTGATAC AGGCAATTCG TTGGGAGACA GTTGTGAAGG AGATGGGTCC TACTTCCCAG 
GCAACTCTCC GAGTGACATC CCTGAAAGCA ACACTGGCTA CTACACTTTC TTCAAGAGTT 
TTACTGTCAG CACTCAACAC AGTTTACAAG CAAATTGGAA AGAACTGGAA GAATCGAATA 
GGTCCATTTA TGAGCCTCCT TCAAGAGCAC ATTGCAGTAA TGGAGAAGGA AGACCTCAAC 
TCTCATCAGT CTCAGTTAAC AGCATTTTTC TTGGAAGCCT TGAACTTCAG ATCATTACAT 
TCAGAGGATA ACCTAGAAGA AGTTGGTCAA ACTGAAGGTT GTATAGTTAG CTGTCTAATG 
AGCATGGTTA TGAAACTTTC TGAAGTTACT TTCAGACCTC TCTTCTTTAA GCTATTTGAT 
TGGGCCAGAA CAGAAGATGC TCCAAAGGAC AGGCTGCTGA CATTCTGCAA CTTGGCAGAT 
TGTATTGCTG AAAAATTGAA AGGCCTTTTT ACCCTATTTG CTGGTCATTT AGTGAAACCT 
TTTGCTGACA CTTTGAACCA AATCAACATC TCTAAAACAG ACGAAGCCTT TTTTGACTCT 
GGAAATGAAC CTGAAAAGAG CTGTTTGTTA CTGCAGTCTA TATTGGATTG TCTGCACAAA 
ATCTTTCTTT TTGACACTCA GCATTTTGTA AGCAAAGAGA GAGCTGAAGC ACTGATGATG 
CCTCTGGTGG ATCAGCTGGA AAACATGCTT GGAGGAGAAG AGACATTTCA GGAAAGAGTG 
ACGAAGCACC TAATACCGTG TATTGCTCAG TTTTCTGTAG CCATGGCAGA TGATTCTCTT 
TGGAAACCAT TGAACTACCA AATTCTCCTT AAGATGCGGC ATTCTTCACC TAAGGTTCGA 
TTCGCTGCCT TGATTACAGT TTTAGAGCTG GCTGAAAAAT TAAAGGAGAA CTACATCGTG 
CTTCTCCCTG AATCCATTCC TTTCTTAGCA GAGCTGATGG AAGATGAATG TGAAGAAGTG 
GAAAATCAGT GCCAGAAGAC TATTCAACAA CTGGAAGTCA TCTTGGGAGA GCCACTCCAG 
AGCTATTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR1 ( XM_023502057.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023357825.1 Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X1, mRNA.

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CloneID	OSa154067
Clone ID Related Accession (Same CDS sequence)	XM_023502057.2 , XM_023502057.1
Accession Version	XM_023502057.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAGA AACTGAGGAA CTGGACAGTC ATATATATGC TAGACTCTTA 
GAAGATATAC TTAAAGATAT ATCATTGGAA AACAACTTAG ACCACTTGTT GGCTAGCACT 
CTTCTTGAAG AATATATTTC ATATAGTTCA AGGAAAGAAA TAGATTCTAA GGAGATATCA 
CTACTCAATG AACAACTCCT TCCTCTCATT AGACTTTTGG AAAGCAAATA CCCTAAGACA 
TTAGATTTGG TATTGGAAGA CCATCTGAAG GATGTTACAG ATCCCCAAAA GCAAGAATTT 
TTTCATCAAT TTATTTCTCT CTCCACAAGT GGAGGAAAGT TTCAGTTTTT AGCAGATTCA 
GATTCTTGCC TCACGCTGAG CTTGAACCAT CCACTGGCTC CAGTGAGACT TTTGGCAGTT 
AACCATCTGA AAAATATTAT GGAAACATCA AAGGACAATA TTGATGCTTC TTTCATAAAA 
GAAGCTATTA TAATGCGTCT TGGTGATGAT AATTTAGATG TTGTTCTGTC AGCCATGAAT 
GCTTTTGTGG TTTTCAAAAA AGAGTTTAGC CCAGAAGAAA CAGTTTCAAG TCTTTTGAGA 
CTTTTTCAAA GAGCAGAACT TTCACAGGAT GGGGCATGGT ACAAAATACT TGAATTAGCA 
GCTGACACTC TAATTAAAGA AGAGATTCTG AATGGAAATG ATGAGTTACT AAATCAAGTG 
GTCATACATT TGCTGCCATT TATGGTTGTT ACCAATGACA ATTCAAAATC CCAAGAAATA 
AAAATGGCTA CTTATCTGTC AAAATCAGGA ATTTGCTCTT TGCACCCTAT ACTGAAAGGA 
TGGAAAGAAG CTCTTGAAAA AGTGATTAAA ACCACAAAGC CAACAGAATT GATTGGTGTA 
GCAAATCAGA AAATGGTTCA GTTGTTGGCT GACAATATAA ATTCAGGGAA TCCTTCTTCA 
ATGTTAAAGA TGGTGGAAGA TTTGATAGCT GTTGGTGAAA AGGAAAGCTA CACATTGAAG 
CAGAAAGTAA CTTCCCAAGT TGTCATTTCA GTTCTTGTCT CTTGCTGCTC TTCATTAAAG 
GAAACTCACT TTCCATTTGC ATTGAAAGTC TTCAATTTGC TGGAAAGGAA AATAAGAGAA 
AATGAAGTTA TTACTTCGTT GGAAATCTCT TTTGAATGTC ATTCTGAACT GTCAGACAAA 
GGAATACCAG TGGAGATATG GATTCAGTAT GTAGAAAAGC TCAACAGAGG GCAGATGATT 
GCTGTGGAAG ATGCTGTTAT GCTTGCATTT TCATTGAAAA ACTTTATTCA AGTCTTGAAG 
CCTCCTAAAT CTTTTCTTAA AGATGATAAG TGGTGGAATC CTGAACATAT GAAAGAAGAC 
AGCCGAGACT ATCTGCACTT ATTGATCCAG ATATTTGAGG TTATTATTGC TGGTGCTGAT 
GCCCTTTGCT TCAGAGTTCT GATGAAACTT TTCTTAAAGG TACATTTAGA AGATGTTTTT 
GAGTTATTCA AGTTCTTCTC ACTTTTATGG ACCTATAATT ATAGCCTTTC AAATCCACTT 
AACTATAATG TGAAAACAAT ACTACAGACT CAAGCTCTTT ACATAGGACG TGCAATGCTG 
GCAGGTCAGG CTGCACAGAA GAAAAAATAC CTGGCATCAA CATCTTCTCC AGTGGTGATA 
TGTTTACTCA TAAATTTGGG AAGTCCTATA AGAGAAGTAC GAAGAGCTGC CATTCTCTGC 
CTCCAGACCT TACGTGAAGT AGAGTCTCCA CTTTATCCTG TAATACAACA CCTAGTCCTA 
AAAGAAGAGG AGATTATTTC AGATGCTACA TATGTAGCAC AGGATTTGAA TACTTTATTT 
GAAGATCTAC AAACAGAAAA AAAGCCAAAA TCTCACCAGA CATTAACTGA AGCCTTAAAA 
CATTTGCTTT ATTGTGTACA TAGCTGTCCA TCCTATATTG CAAAAAACTT GATGAAAATT 
CTTCAGGAAG TCAATAGTGA GACGGTGCTT TCTCAGCTGT TGCCCACAAT TGATCAGTTG 
CTAGAGAAAG TCCTAAAGGA ACCCCCTACA GTCCTGAAGG ATGAAGCTGT CATTTTGCAC 
CTTGCCCTTG GGAAATACAA TGAGCATTCA GCTTCTCTCT TGCACAAGGA CCCACAGAGT 
TTAGATATAT TCCTCAAAGC TGTGCATACC ACCAAGGAAG TCTTCGAAGG GATGCCTACA 
TTCCAGATCC TTGCCCTTGG GAAGATTACA AAACCATTCT TTGCTGCTAT ATCAGATGAC 
AAAGTTCAAC AGAAGCTTCT GGGAATGCTG TTTGATTTAC TTTTGAATTG CAAAAATACA 
CTCTGTGCTC AGACTGTTAG CAGTGTTTTT AAAACAATTT CTGTTGATGC TGAACAAATC 
CGATTAGAAC TAGAACCACC AGAGAAAAAC AAGTCCTTGG GTACAATTCA GCAAACAAGA 
AGACAGAAAA TGCAACAGAA AAAACTACAA GATACAGAAC CTGCACAAGA TGTTGGCAGT 
GCTTACTGGC AGAGAGTAAC CCTCATCTTA GAATTACTGC AGCACAAAAA GAAACTCAAA 
AGTCCTCAGT TATTAGTGCC ATGTCTTTTT AATTTGCTCT CCAGGTGTCT AGAAACTTTG 
CCACCGGAGC AGGGAAATCT GGAATATACC AAACAGCTAA TTCTTAGCTG TCTACTTAAT 
ATCTGCCAGA AACTATCCCC AGATGGTTCT AAGATCCCTA AAGGTATTCT TGATGAAGAA 
AAATTCAATG TTGAATTGTT AGTTCAGTGT GTGCGGGTTT CAGAGATGCC TCAGACACAT 
CACCATGCCC TCTTGCTTTT GGGTGCAGTT GCTGGAATAT TTCCTGATAA AGTTTTGCAC 
AACATTATGC CCATCTTTAC ATTCATGGGA GCCAATGTTA TGCGCCTTGA TGACACTTAC 
AGTTTTCAAG TTATCAACAA GACAGTGAAA ATGGTCATTC CTGCTCTTAT CCAGGCAGAC 
AGTGGTAAGT CTGCAGATGT CACAAGGAAC ATAGAAGAAG TTGTGGTTAA AATTATAAAT 
GTGTTTGTGG ATGCTCTACC TCATGTTCCC GAACATAGAC GCCTGCCCAT CCTTGTACAA 
CTTGTTGATA CTCTTGGTGC AGAGAAATTC CTCTGGATTC TCCTGCTGTT GCTTTTTGAA 
CAGTATGTCA CTAAAACAGT GTTGGTTGCT GCTCATGGAG AAAAGGATGC TATCTTAGAG 
ACTGACACTG AATTTTGGAT ATCAGTCTCT TGTGAATTTA CTGTCCAACA GCAGCTCCAA 
AGTTTAATGA ATATACTCAG ATACTTAACC AAACTTCCAG AGAAAAAAGA AGGAGAAGAA 
CCTCCCAAAA CAGGATCTCA CAACTCCAAG ACACTGGAAC AGATGGTACA GGTTTTCAAT 
GTGGACACTC ATACCAGCAA GCAGCTCCGA CATTTTACAT TTTTGTCTGT GTCCTTTATG 
TCTCAACTCT TGGCTTCCCA TAATTTTATC AAAAAGGTCA TGGAAAATGG TGGTACTGTG 
GCTTTACAAG GCCTTGAACA GAGATTACTT GAAACTGTTC TTGGTTATAT AAATTCTGTG 
GCACAGTCAG TGGAGGAAAA TGCAGACAAA TTAACTGCAA AGTTTTGGCG TGCTCTCTTG 
AGTAAAGCTT ATGATTTGCT TGATAAAGTC AATTCCTTAT TGCCCACAGA AACTTTTGTT 
CCTGTGATAA GAGGATTGCT GACTAACCAA CTGCCTTCTG TCCGTCGTAA AGCAATGGAC 
CTCTTAAACA ATAAATTGCA TCAGTATGCA AAGTGGAAAA AGGGACAGGT GACCCTTTTC 
TTGAAACTGG TTCCAGATCT CTTGGATATT GCACAGCATA AAATAAAGGA GAAAGAAGAA 
GAACAGGCCA TCAACAGACA GACTGCCTTG TACAGTTTGA AATTACTCTG CAAAAATTTT 
GGCACAGAAA ATTCAGAGGT TTTTATCCCT GTATTGAGTG CAGCCATTAA CCTGATCAAT 
CCAGAGGTGA AGGATGAGAA GAACGTATTG GGAAGTGCTT TGTTGTGTAT AGCTGAGGTC 
ACCTACGTAC TGGGAGCATT AGCAATACCT CATCTTCCCA GACTTATGCC TGCATTATTG 
ACTACCCTGG AGAATACGAA CGAGCTGATC TCAAGTGAGG TTTACTTGCT TAGTGCATTG 
GCAGCCCTTC AGAAAGTTGT TGAAACTCTT CCACATTTTC TCAGCCCATA CCTACAGGGT 
ATTTTGATAC AGGCAATTCG TTGGGAGACA GTTGTGAAGG AGATGGGTCC TACTTCCCAG 
GCAACTCTCC GAGTGACATC CCTGAAAGCA ACACTGGCTA CTACACTTTC TTCAAGAGTT 
TTACTGTCAG CACTCAACAC AGTTTACAAG CAAATTGGAA AGAACTGGAA GAATCGAATA 
GGTCCATTTA TGAGCCTCCT TCAAGAGCAC ATTGCAGTAA TGGAGAAGGA AGACCTCAAC 
TCTCATCAGT CTCAGTTAAC AGCATTTTTC TTGGAAGCCT TGAACTTCAG ATCATTACAT 
TCAGAGGATA ACCTAGAAGA AGTTGGTCAA ACTGAAGGTT GTATAGTTAG CTGTCTAATG 
AGCATGGTTA TGAAACTTTC TGAAGTTACT TTCAGACCTC TCTTCTTTAA GCTATTTGAT 
TGGGCCAGAA CAGAAGATGC TCCAAAGGAC AGGCTGCTGA CATTCTGCAA CTTGGCAGAT 
TGTATTGCTG AAAAATTGAA AGGCCTTTTT ACCCTATTTG CTGGTCATTT AGTGAAACCT 
TTTGCTGACA CTTTGAACCA AATCAACATC TCTAAAACAG ACGAAGCCTT TTTTGACTCT 
GGAAATGAAC CTGAAAAGAG CTGTTTGTTA CTGCAGTCTA TATTGGATTG TCTGCACAAA 
ATCTTTCTTT TTGACACTCA GCATTTTGTA AGCAAAGAGA GAGCTGAAGC ACTGATGATG 
CCTCTGGTGG ATCAGCTGGA AAACATGCTT GGAGGAGAAG AGACATTTCA GGAAAGAGTG 
ACGAAGCACC TAATACCGTG TATTGCTCAG TTTTCTGTAG CCATGGCAGA TGATTCTCTT 
TGGAAACCAT TGAACTACCA AATTCTCCTT AAGATGCGGC ATTCTTCACC TAAGGTTCGA 
TTCGCTGCCT TGATTACAGT TTTAGAGCTG GCTGAAAAAT TAAAGGAGAA CTACATCGTG 
CTTCTCCCTG AATCCATTCC TTTCTTAGCA GAGCTGATGG AAGATGAATG TGAAGAAGTG 
GAAAATCAGT GCCAGAAGAC TATTCAACAA CTGGAAGTCA TCTTGGGAGA GCCACTCCAG 
AGCTATTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023357825.1
CDS	90..6521
Translation	MTSLSQQLQRLALPQNDPSLLARHEVASLLFDCKEAASIDRDTIFAIGCTGLEELIGIDPSFQKFESSLFSQLAKSLERSVQTKAVNKQLDENISLFLIHLSPYFMLKPAQKCLEWLIHRFHIHLYNQDSLIGCVLPYHETKIFVRVIQLLKISSPTHKWNWLEPVKMSGVHLAKGTLVTHCYKDLGFMDFICSLVTKSVKVFSEYPGSSAQLRVLLTFYASTIVSALVSANDISDSLLAKLFPYIQKGLKSSLPDYRAATYMIICQVSVKVTMEDTFVHSLASQIGKTLTKIPSLIKNGLGCLIVLLQRQKVEGLGKKLFAHLCNVPDLVSLLHEISVTYDISPLLHYMLPHLVVTIVHHVTEEEETEELDSHIYARLLEDILKDISLENNLDHLLASTLLEEYISYSSRKEIDSKEISLLNEQLLPLIRLLESKYPKTLDLVLEDHLKDVTDPQKQEFFHQFISLSTSGGKFQFLADSDSCLTLSLNHPLAPVRLLAVNHLKNIMETSKDNIDASFIKEAIIMRLGDDNLDVVLSAMNAFVVFKKEFSPEETVSSLLRLFQRAELSQDGAWYKILELAADTLIKEEILNGNDELLNQVVIHLLPFMVVTNDNSKSQEIKMATYLSKSGICSLHPILKGWKEALEKVIKTTKPTELIGVANQKMVQLLADNINSGNPSSMLKMVEDLIAVGEKESYTLKQKVTSQVVISVLVSCCSSLKETHFPFALKVFNLLERKIRENEVITSLEISFECHSELSDKGIPVEIWIQYVEKLNRGQMIAVEDAVMLAFSLKNFIQVLKPPKSFLKDDKWWNPEHMKEDSRDYLHLLIQIFEVIIAGADALCFRVLMKLFLKVHLEDVFELFKFFSLLWTYNYSLSNPLNYNVKTILQTQALYIGRAMLAGQAAQKKKYLASTSSPVVICLLINLGSPIREVRRAAILCLQTLREVESPLYPVIQHLVLKEEEIISDATYVAQDLNTLFEDLQTEKKPKSHQTLTEALKHLLYCVHSCPSYIAKNLMKILQEVNSETVLSQLLPTIDQLLEKVLKEPPTVLKDEAVILHLALGKYNEHSASLLHKDPQSLDIFLKAVHTTKEVFEGMPTFQILALGKITKPFFAAISDDKVQQKLLGMLFDLLLNCKNTLCAQTVSSVFKTISVDAEQIRLELEPPEKNKSLGTIQQTRRQKMQQKKLQDTEPAQDVGSAYWQRVTLILELLQHKKKLKSPQLLVPCLFNLLSRCLETLPPEQGNLEYTKQLILSCLLNICQKLSPDGSKIPKGILDEEKFNVELLVQCVRVSEMPQTHHHALLLLGAVAGIFPDKVLHNIMPIFTFMGANVMRLDDTYSFQVINKTVKMVIPALIQADSGKSADVTRNIEEVVVKIINVFVDALPHVPEHRRLPILVQLVDTLGAEKFLWILLLLLFEQYVTKTVLVAAHGEKDAILETDTEFWISVSCEFTVQQQLQSLMNILRYLTKLPEKKEGEEPPKTGSHNSKTLEQMVQVFNVDTHTSKQLRHFTFLSVSFMSQLLASHNFIKKVMENGGTVALQGLEQRLLETVLGYINSVAQSVEENADKLTAKFWRALLSKAYDLLDKVNSLLPTETFVPVIRGLLTNQLPSVRRKAMDLLNNKLHQYAKWKKGQVTLFLKLVPDLLDIAQHKIKEKEEEQAINRQTALYSLKLLCKNFGTENSEVFIPVLSAAINLINPEVKDEKNVLGSALLCIAEVTYVLGALAIPHLPRLMPALLTTLENTNELISSEVYLLSALAALQKVVETLPHFLSPYLQGILIQAIRWETVVKEMGPTSQATLRVTSLKATLATTLSSRVLLSALNTVYKQIGKNWKNRIGPFMSLLQEHIAVMEKEDLNSHQSQLTAFFLEALNFRSLHSEDNLEEVGQTEGCIVSCLMSMVMKLSEVTFRPLFFKLFDWARTEDAPKDRLLTFCNLADCIAEKLKGLFTLFAGHLVKPFADTLNQINISKTDEAFFDSGNEPEKSCLLLQSILDCLHKIFLFDTQHFVSKERAEALMMPLVDQLENMLGGEETFQERVTKHLIPCIAQFSVAMADDSLWKPLNYQILLKMRHSSPKVRFAALITVLELAEKLKENYIVLLPESIPFLAELMEDECEEVENQCQKTIQQLEVILGEPLQSYF

Target ORF information:

RefSeq Version	XM_023502057.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 1 (HEATR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023502057.1

ORF Insert Sequence:

ATGACATCTT TATCGCAGCA ACTACAACGT CTTGCTCTCC CTCAAAATGA TCCCAGTCTC 
TTGGCTAGGC ATGAGGTTGC ATCCTTACTA TTTGATTGTA AGGAAGCAGC CAGCATTGAC 
AGAGATACCA TCTTTGCTAT AGGATGTACT GGACTTGAAG AGCTTATTGG AATCGATCCT 
TCATTTCAGA AGTTTGAGTC TTCTTTGTTT AGCCAGTTGG CGAAAAGTTT GGAGAGGAGT 
GTTCAGACTA AGGCAGTAAA CAAACAACTG GATGAAAATA TCTCATTATT TCTTATACAC 
TTGTCTCCTT ATTTCATGCT TAAACCAGCA CAGAAGTGCC TAGAGTGGTT GATTCACAGA 
TTTCATATAC ATCTTTACAA TCAAGATAGC CTGATTGGTT GTGTTCTTCC TTATCATGAG 
ACAAAAATAT TTGTCCGTGT TATACAGCTT CTAAAAATTA GTAGTCCAAC ACATAAATGG 
AATTGGTTGG AACCTGTTAA GATGTCTGGG GTTCATTTAG CCAAAGGAAC TTTGGTTACC 
CATTGCTACA AAGACCTTGG TTTCATGGAT TTTATATGCA GTCTGGTAAC CAAATCTGTA 
AAGGTTTTTT CAGAGTACCC TGGAAGTTCA GCTCAGTTAA GAGTCCTACT GACTTTTTAT 
GCTTCCACTA TTGTGTCAGC TTTGGTGTCT GCTAATGATA TATCAGACTC TTTACTTGCT 
AAGCTCTTCC CCTATATTCA AAAGGGACTA AAGTCATCTT TGCCTGATTA TAGAGCTGCA 
ACCTACATGA TAATCTGTCA AGTCTCAGTG AAAGTGACAA TGGAGGATAC TTTTGTTCAT 
TCCTTGGCAT CACAGATTGG CAAAACTTTG ACCAAAATTC CCTCTTTGAT CAAGAATGGA 
TTAGGTTGCT TGATTGTGCT CTTGCAGAGA CAGAAAGTAG AAGGCCTTGG GAAAAAGCTA 
TTTGCTCACC TGTGTAATGT TCCTGACCTT GTTTCCTTAC TACATGAAAT TTCAGTGACG 
TATGACATTA GCCCTCTTCT GCATTACATG CTTCCTCATC TTGTTGTCAC CATTGTTCAT 
CATGTTACAG AAGAAGAAGA AACTGAGGAA CTGGACAGTC ATATATATGC TAGACTCTTA 
GAAGATATAC TTAAAGATAT ATCATTGGAA AACAACTTAG ACCACTTGTT GGCTAGCACT 
CTTCTTGAAG AATATATTTC ATATAGTTCA AGGAAAGAAA TAGATTCTAA GGAGATATCA 
CTACTCAATG AACAACTCCT TCCTCTCATT AGACTTTTGG AAAGCAAATA CCCTAAGACA 
TTAGATTTGG TATTGGAAGA CCATCTGAAG GATGTTACAG ATCCCCAAAA GCAAGAATTT 
TTTCATCAAT TTATTTCTCT CTCCACAAGT GGAGGAAAGT TTCAGTTTTT AGCAGATTCA 
GATTCTTGCC TCACGCTGAG CTTGAACCAT CCACTGGCTC CAGTGAGACT TTTGGCAGTT 
AACCATCTGA AAAATATTAT GGAAACATCA AAGGACAATA TTGATGCTTC TTTCATAAAA 
GAAGCTATTA TAATGCGTCT TGGTGATGAT AATTTAGATG TTGTTCTGTC AGCCATGAAT 
GCTTTTGTGG TTTTCAAAAA AGAGTTTAGC CCAGAAGAAA CAGTTTCAAG TCTTTTGAGA 
CTTTTTCAAA GAGCAGAACT TTCACAGGAT GGGGCATGGT ACAAAATACT TGAATTAGCA 
GCTGACACTC TAATTAAAGA AGAGATTCTG AATGGAAATG ATGAGTTACT AAATCAAGTG 
GTCATACATT TGCTGCCATT TATGGTTGTT ACCAATGACA ATTCAAAATC CCAAGAAATA 
AAAATGGCTA CTTATCTGTC AAAATCAGGA ATTTGCTCTT TGCACCCTAT ACTGAAAGGA 
TGGAAAGAAG CTCTTGAAAA AGTGATTAAA ACCACAAAGC CAACAGAATT GATTGGTGTA 
GCAAATCAGA AAATGGTTCA GTTGTTGGCT GACAATATAA ATTCAGGGAA TCCTTCTTCA 
ATGTTAAAGA TGGTGGAAGA TTTGATAGCT GTTGGTGAAA AGGAAAGCTA CACATTGAAG 
CAGAAAGTAA CTTCCCAAGT TGTCATTTCA GTTCTTGTCT CTTGCTGCTC TTCATTAAAG 
GAAACTCACT TTCCATTTGC ATTGAAAGTC TTCAATTTGC TGGAAAGGAA AATAAGAGAA 
AATGAAGTTA TTACTTCGTT GGAAATCTCT TTTGAATGTC ATTCTGAACT GTCAGACAAA 
GGAATACCAG TGGAGATATG GATTCAGTAT GTAGAAAAGC TCAACAGAGG GCAGATGATT 
GCTGTGGAAG ATGCTGTTAT GCTTGCATTT TCATTGAAAA ACTTTATTCA AGTCTTGAAG 
CCTCCTAAAT CTTTTCTTAA AGATGATAAG TGGTGGAATC CTGAACATAT GAAAGAAGAC 
AGCCGAGACT ATCTGCACTT ATTGATCCAG ATATTTGAGG TTATTATTGC TGGTGCTGAT 
GCCCTTTGCT TCAGAGTTCT GATGAAACTT TTCTTAAAGG TACATTTAGA AGATGTTTTT 
GAGTTATTCA AGTTCTTCTC ACTTTTATGG ACCTATAATT ATAGCCTTTC AAATCCACTT 
AACTATAATG TGAAAACAAT ACTACAGACT CAAGCTCTTT ACATAGGACG TGCAATGCTG 
GCAGGTCAGG CTGCACAGAA GAAAAAATAC CTGGCATCAA CATCTTCTCC AGTGGTGATA 
TGTTTACTCA TAAATTTGGG AAGTCCTATA AGAGAAGTAC GAAGAGCTGC CATTCTCTGC 
CTCCAGACCT TACGTGAAGT AGAGTCTCCA CTTTATCCTG TAATACAACA CCTAGTCCTA 
AAAGAAGAGG AGATTATTTC AGATGCTACA TATGTAGCAC AGGATTTGAA TACTTTATTT 
GAAGATCTAC AAACAGAAAA AAAGCCAAAA TCTCACCAGA CATTAACTGA AGCCTTAAAA 
CATTTGCTTT ATTGTGTACA TAGCTGTCCA TCCTATATTG CAAAAAACTT GATGAAAATT 
CTTCAGGAAG TCAATAGTGA GACGGTGCTT TCTCAGCTGT TGCCCACAAT TGATCAGTTG 
CTAGAGAAAG TCCTAAAGGA ACCCCCTACA GTCCTGAAGG ATGAAGCTGT CATTTTGCAC 
CTTGCCCTTG GGAAATACAA TGAGCATTCA GCTTCTCTCT TGCACAAGGA CCCACAGAGT 
TTAGATATAT TCCTCAAAGC TGTGCATACC ACCAAGGAAG TCTTCGAAGG GATGCCTACA 
TTCCAGATCC TTGCCCTTGG GAAGATTACA AAACCATTCT TTGCTGCTAT ATCAGATGAC 
AAAGTTCAAC AGAAGCTTCT GGGAATGCTG TTTGATTTAC TTTTGAATTG CAAAAATACA 
CTCTGTGCTC AGACTGTTAG CAGTGTTTTT AAAACAATTT CTGTTGATGC TGAACAAATC 
CGATTAGAAC TAGAACCACC AGAGAAAAAC AAGTCCTTGG GTACAATTCA GCAAACAAGA 
AGACAGAAAA TGCAACAGAA AAAACTACAA GATACAGAAC CTGCACAAGA TGTTGGCAGT 
GCTTACTGGC AGAGAGTAAC CCTCATCTTA GAATTACTGC AGCACAAAAA GAAACTCAAA 
AGTCCTCAGT TATTAGTGCC ATGTCTTTTT AATTTGCTCT CCAGGTGTCT AGAAACTTTG 
CCACCGGAGC AGGGAAATCT GGAATATACC AAACAGCTAA TTCTTAGCTG TCTACTTAAT 
ATCTGCCAGA AACTATCCCC AGATGGTTCT AAGATCCCTA AAGGTATTCT TGATGAAGAA 
AAATTCAATG TTGAATTGTT AGTTCAGTGT GTGCGGGTTT CAGAGATGCC TCAGACACAT 
CACCATGCCC TCTTGCTTTT GGGTGCAGTT GCTGGAATAT TTCCTGATAA AGTTTTGCAC 
AACATTATGC CCATCTTTAC ATTCATGGGA GCCAATGTTA TGCGCCTTGA TGACACTTAC 
AGTTTTCAAG TTATCAACAA GACAGTGAAA ATGGTCATTC CTGCTCTTAT CCAGGCAGAC 
AGTGGTAAGT CTGCAGATGT CACAAGGAAC ATAGAAGAAG TTGTGGTTAA AATTATAAAT 
GTGTTTGTGG ATGCTCTACC TCATGTTCCC GAACATAGAC GCCTGCCCAT CCTTGTACAA 
CTTGTTGATA CTCTTGGTGC AGAGAAATTC CTCTGGATTC TCCTGCTGTT GCTTTTTGAA 
CAGTATGTCA CTAAAACAGT GTTGGTTGCT GCTCATGGAG AAAAGGATGC TATCTTAGAG 
ACTGACACTG AATTTTGGAT ATCAGTCTCT TGTGAATTTA CTGTCCAACA GCAGCTCCAA 
AGTTTAATGA ATATACTCAG ATACTTAACC AAACTTCCAG AGAAAAAAGA AGGAGAAGAA 
CCTCCCAAAA CAGGATCTCA CAACTCCAAG ACACTGGAAC AGATGGTACA GGTTTTCAAT 
GTGGACACTC ATACCAGCAA GCAGCTCCGA CATTTTACAT TTTTGTCTGT GTCCTTTATG 
TCTCAACTCT TGGCTTCCCA TAATTTTATC AAAAAGGTCA TGGAAAATGG TGGTACTGTG 
GCTTTACAAG GCCTTGAACA GAGATTACTT GAAACTGTTC TTGGTTATAT AAATTCTGTG 
GCACAGTCAG TGGAGGAAAA TGCAGACAAA TTAACTGCAA AGTTTTGGCG TGCTCTCTTG 
AGTAAAGCTT ATGATTTGCT TGATAAAGTC AATTCCTTAT TGCCCACAGA AACTTTTGTT 
CCTGTGATAA GAGGATTGCT GACTAACCAA CTGCCTTCTG TCCGTCGTAA AGCAATGGAC 
CTCTTAAACA ATAAATTGCA TCAGTATGCA AAGTGGAAAA AGGGACAGGT GACCCTTTTC 
TTGAAACTGG TTCCAGATCT CTTGGATATT GCACAGCATA AAATAAAGGA GAAAGAAGAA 
GAACAGGCCA TCAACAGACA GACTGCCTTG TACAGTTTGA AATTACTCTG CAAAAATTTT 
GGCACAGAAA ATTCAGAGGT TTTTATCCCT GTATTGAGTG CAGCCATTAA CCTGATCAAT 
CCAGAGGTGA AGGATGAGAA GAACGTATTG GGAAGTGCTT TGTTGTGTAT AGCTGAGGTC 
ACCTACGTAC TGGGAGCATT AGCAATACCT CATCTTCCCA GACTTATGCC TGCATTATTG 
ACTACCCTGG AGAATACGAA CGAGCTGATC TCAAGTGAGG TTTACTTGCT TAGTGCATTG 
GCAGCCCTTC AGAAAGTTGT TGAAACTCTT CCACATTTTC TCAGCCCATA CCTACAGGGT 
ATTTTGATAC AGGCAATTCG TTGGGAGACA GTTGTGAAGG AGATGGGTCC TACTTCCCAG 
GCAACTCTCC GAGTGACATC CCTGAAAGCA ACACTGGCTA CTACACTTTC TTCAAGAGTT 
TTACTGTCAG CACTCAACAC AGTTTACAAG CAAATTGGAA AGAACTGGAA GAATCGAATA 
GGTCCATTTA TGAGCCTCCT TCAAGAGCAC ATTGCAGTAA TGGAGAAGGA AGACCTCAAC 
TCTCATCAGT CTCAGTTAAC AGCATTTTTC TTGGAAGCCT TGAACTTCAG ATCATTACAT 
TCAGAGGATA ACCTAGAAGA AGTTGGTCAA ACTGAAGGTT GTATAGTTAG CTGTCTAATG 
AGCATGGTTA TGAAACTTTC TGAAGTTACT TTCAGACCTC TCTTCTTTAA GCTATTTGAT 
TGGGCCAGAA CAGAAGATGC TCCAAAGGAC AGGCTGCTGA CATTCTGCAA CTTGGCAGAT 
TGTATTGCTG AAAAATTGAA AGGCCTTTTT ACCCTATTTG CTGGTCATTT AGTGAAACCT 
TTTGCTGACA CTTTGAACCA AATCAACATC TCTAAAACAG ACGAAGCCTT TTTTGACTCT 
GGAAATGAAC CTGAAAAGAG CTGTTTGTTA CTGCAGTCTA TATTGGATTG TCTGCACAAA 
ATCTTTCTTT TTGACACTCA GCATTTTGTA AGCAAAGAGA GAGCTGAAGC ACTGATGATG 
CCTCTGGTGG ATCAGCTGGA AAACATGCTT GGAGGAGAAG AGACATTTCA GGAAAGAGTG 
ACGAAGCACC TAATACCGTG TATTGCTCAG TTTTCTGTAG CCATGGCAGA TGATTCTCTT 
TGGAAACCAT TGAACTACCA AATTCTCCTT AAGATGCGGC ATTCTTCACC TAAGGTTCGA 
TTCGCTGCCT TGATTACAGT TTTAGAGCTG GCTGAAAAAT TAAAGGAGAA CTACATCGTG 
CTTCTCCCTG AATCCATTCC TTTCTTAGCA GAGCTGATGG AAGATGAATG TGAAGAAGTG 
GAAAATCAGT GCCAGAAGAC TATTCAACAA CTGGAAGTCA TCTTGGGAGA GCCACTCCAG 
AGCTATTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HEATR1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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