COL5A3 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A3
Entrez Gene ID	100913921
Full Name	collagen type V alpha 3 chain
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012541920.2	XP_012397374.1	collagen alpha-3(V) chain isoform X2
XM_023496595.1	XP_023352363.1	collagen alpha-3(V) chain isoform X1
XM_012541920.1	XP_012397374.1	collagen alpha-3(V) chain
XM_023496595.2	XP_023352363.2	collagen alpha-3(V) chain

Pathways from BioSystems
Gene Ontology

The following COL5A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa122168	XM_012541920.2 Latest version!	Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa158903	XM_023496595.1 Latest version!	Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa221996	XM_023496595.2 Latest version!	Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OSa122168	XM_012541920.1	Sarcophilus harrisii collagen, type V, alpha 3 (COL5A3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A3 ( XM_012541920.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012397374.1 Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.

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CloneID	OSa122168
Clone ID Related Accession (Same CDS sequence)	XM_012541920.2 , XM_012541920.1
Accession Version	XM_012541920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-3(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823414.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012541920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGATG GGACCGGTAG CCGAGAACGG GGCTGGTCCA GATCCTGGGC AGATTTTTTC 
CTGTTCCTCA CTTCCCTGAT GCAACTGCCC CAAGGGGGAA TTGCTGATCC TGTAGATGTG 
CTTCAAGAAT TGGGAATCAA GGAAGGCAAA GCCCAGGTCC CCGAAGTGCC TGGCTTCTGC 
CCTCAGAGGT TCCCAGAAGG AGATCGAGCA TTCTGGGTGG GGAACTCCAG CCAACTCAGT 
GTTCCTGTGG CGAAGCTTTG GCCAGCTGGT CACTTTCCAG AGAACTTTTC ACTGCTGATC 
ACCCTGAGGG GCTATTCTTC CGCTCAGGCA GTCCTCCTGT CCATTTATGA TGAAAGGGGA 
GTGAGGCAAC TGGGACTAGG AGTGGGGCGG CCAACCCAGC TCCTGTCTGA CCCACCCAGC 
CCCAGCCTGC TTCCTTCAGC AGTTGACCTC ATGGATGGCA GGTGGCACCG TGTGGCGGTC 
AGTGTGGACA GCTGGACAGT GACTCTGGTG GTTGACTGTG AGCCACAGCC CCCAGTGAGC 
TGGGGCCGGC AGAGATTGAC CCCTATCAGC ATAGCTGGGT TCATTATGCT GGGGACACAG 
GACTCTGGAG AAGAGACCTT TCAGGGGGAT GTCCAGGACC TTATGATCCT GCAAGATGCT 
CAGGCTGCCT TCAGGGCCTG TGAAAACTTG CTGCCAGGGT GTGATACGCC CACTACCTCA 
GCTACCCCAT CTACCCCAGT TGAGCCAGAT ACCTCTCCTC CCCAAAGGAA AGGGAAGGGG 
AGAGGGAAGG GAAAGGGCCG GAAAGGCCGA AAGGGCAAGG GCAAGAAGAA GAAGAAAAAC 
AAGGAGTCTG AGAAACCAGA AGTGCCTCCA ATTTCACCAG AGGGCCAGAC CATCACTCAC 
ATTGCTGAGA GAGAGACTCT AACTCTCCTC CCTGAGTCAG AGCCTGTGGC CCCAACTGTC 
CCCTCAAATG TCACAGTCAT CGCATCCACA GTGGCCATCG ATCTCAATGT CACCACTTAT 
AAGGACCCTG GAATTGAGTC AGTGTCTTTT GAAGATACAC AGGAGCTCCC TGAGCCAAGG 
GAGGGAAGAG ATGAAGAGAG GGGAAACTAC ACTATGAACT CCATCTATGG ACCGGCTGAG 
CGTGGACCAG AGTCCCAGAT CTATATATTT CCTAATGCTG GTGAAAAAGG TCAGAAAGGA 
GAGCCAGCAG TGATTGAAGT GGGCCAGCAG TTTGAAGGGT ACCCAGGACT TCCAGGGCCT 
TTGGGAATAG TTGGTCCTTC AGGTCCTCCT GGTCCACCTG GCTTCCCTGG AGACCCAGGG 
GAAAGGGGTC CTGCTGGTCG TCCTGGTCTC CCTGGAGTGG ATGGTATCCG AGGCCTCCCA 
GGGACTGTAA TAATGATGCC TTTCCACTTT GGGGGTGAGT CCCTCAAGGG TCCCCCAAGC 
TCCTTCCAAC AGGCACAGGC ACAGGCAGTG CTACAGCAAA CCCAGCTCTC CATGCAAGGC 
CCTCCTGGCC CTATCGGGCT TACAGGTCGA GCTGGACCCT TGGGTCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAACCAGG GATAGCAGGA CCACAGGGTC CTAGAGGTCT ACAGGGTTTA 
CCTGGGCCCT CTGGAAAAGA AGGAAAACAG GGTCGCTCTG GAGCAGATGG ACCAAGGGGT 
CTCCCAGGGG ATACTGGACC TAAGGGTGAC CGGGGTTTCG ATGGCCTGCC CGGGCTGCCA 
GGAGAGAAAG GCCAAAGGGG AGATGTTGGC CATGTTGGGC AGCCAGGGCC CCCTGGCGAG 
GATGGTAAGA AGGGCTCAGA GGGGCCTCCA GGGCCCAGGG GCCAAGCTGG GGAACCAGGA 
CCCCGGGGGC TTATTGGCGC AAGAGGCTCC CCAGGTGCCC CAGGACAACC AGGTGTGGTG 
GGGATAGATG GACCTCCGGG TGCCAAAGGA AATGTGGGCA TCCCAGGAGA GCCAGGTCCT 
CCAGGTCAGC AAGGAAACCC AGGGTCCCAG GGTCTCCCTG GACCACAGGG ACCAATTGGG 
GAACCAGGGG ACAAGGGGCC CCCAGGAAAG CCAGGAATTC CAGGTCTTCC AGGAGCTGAA 
GGCCCACCGG GTCACCCCGG CCATGAGGGC CCCACAGGCG AGAAAGGGGC CCAGGGTCCA 
GAAGGGTCCA TAGGCCCAAC AGGGTATCCC GGGCAGCGAG GAGTAAAGGG CACATCTGGA 
AACCGAGGCC TAAAGGGGGA GAAAGGCGAG AAGGGAGAAG ATGGCTTCCC AGGCTTTAAG 
GGTGACGCAG GTCTAAAAGG AGATCGAGGT GACCCTGGAC CTCTGGGCCC AAGGGGAGAG 
GATGGGCCCG AGGGGCTGAA GGGTCAGGCA GGATTGCTGG GTGAAGAGGG ACCCCCAGGT 
CCAGCTGGGG AGAAGGGCAA GCTTGGTATC CCAGGCCTCC CAGGTTATCC AGGCCGTCAG 
GGTCCTAAGG GATCCATTGG ATTTCCTGGG CCACTGGGTC TGCCTGGAGA GAAAGGGAAA 
CGGGGGAAGG CTGGTCAGCC AGGACAGGAA GGCGAACGGG GACCACCAGG AGCCCGTGGA 
GAGAGGGGAC AGAATGGTCC CACAGGAAAA CCAGGCCCCA AGGGAGATGT GGGCCATGAT 
GGTTCTGCAG GGTTTCCTGG AGAAAAGGGA TTGCCGGGTC TAAAGGGAGC CCTTGGATTT 
CCTGGGCCAA AAGGACCCCC CGGCCCTGCA GGGAAGGAAG GAAGACCTGG TCACCCTGGG 
CAGCGGGGAG AGTTGGGTTT CCAAGGACAG ACAGGTCCCC CAGGACCAGC TGGTGTCATT 
GGCCCACAGG GGAAGACTGG TGAAACAGGG CCACTCGGTG AAAGGGGCCA TCCAGGCCCT 
CCTGGGCCCC CTGGAGAACA AGGGCTTCCA GGACTTCTGG GAAGAGAAGG AGCTCAGGGG 
GACCCTGGAC CAGCTGGTCT CCCTGGGAAG GATGGCCCCC CTGGTCCCCG AGGCTTCCCA 
GGCCCTCGTG GAGCCCCTGG AGATACAGGA ACAACTGGCC TGAAGGGGTC CCAGGGCCCC 
CCTGGCCCTA TTGGTGCCAA TGGTCCCCCC GGAGAACGAG GTCCTGTGGG CCCTGCTGGG 
GGCATTGGGT TGCCTGGTCA AGGGGGTGGA CAAGGGCCTC TTGGTCCTGC TGGAGAGACA 
GGAGCAGCAG GGGAGCGTGG ACCCCCTGGT CCCACAGGGA AAGATGGAAT TCCAGGGCTT 
CCAGGCCCCT TGGGACCAGC TGGAGCTACA GGGCCGGCTG GGGAAGATGG AGACAAGGGG 
GAAATGGGTG CCCCAGGCCA CAAGGGAAGC AAAGGTGACA AAGGGGAGCC GGGTCCCCCT 
GGGCCAACAG GGATCCAAGG TCCTGCTGGA CACCCAGGAC TTCCAGGTGC GGATGGGGAA 
CGAGGCCGTC GGGGTCCACC AGGCCTCTTT GGGCAGAAAG GAGATGATGG TGTGAGGGGC 
TTCCCAGGAG GCATTGGTCC CCCTGGCCTA CAGGGGATGC CAGGACCACC TGGAGAGAAG 
GGAGAGGTCG GAGATGTGGG CTCCATGGGT CCTCATGGTG CTCCAGGTCC CCGAGGACCT 
CAGGGCCCTA GTGGGTCAGA GGGATCTCCA GGGCTGCCTG GAGGCATTGG TCAACCAGGG 
GCTGTTGGAG AAAAAGGGGA ACCTGGGGAA GTGGGGGATC CTGGTCCCCC AGGCAAGCCT 
GGCCCAGTGG GACCCAAGGG AGAGTTTGGT GAAAAGGGTG ACTCAGGACC AACAGGGGCT 
GCCGGGCCTC CAGGGAAGAA AGGACCTCCT GGTGAAGATG GAGCCAAAGG GAGTCTGGGT 
CCCACAGGAT TCCCCGGAGA CCCTGGTCCC CCTGGAGACC CTGGTGTTGC TGGCAAAGAA 
GGTGTCCAGG GAGTGAAAGG GGACCACGGG GATGTCGGGG CTCCGGGCCC TCCTGGTGCC 
TCTGGAGAAC CTGGGCCTCC AGGGCCTCCT GGGAAAAGGG GGCCTGTTGG CCGGATAGGT 
CAAGAGGGCA GAGAAGGAGA GAAGGGGGCC AAGGGTCAGC CTGGTCCTGA TGGCCCCCCT 
GGAAGGACAG GCCCAGTGGG TCCAAGGGGC CCTCCTGGCC GACTTGGGGC TGAGGGCCTC 
CCAGGGATTC CTGGACCCTC GGGTGAGCCA GGTCTTCTTG GCCCTCCTGG ACAACTGGGT 
CCACCTGGGC CCCTGGGACC CAGTGGTCTC CCTGGCCTTA AAGGAGATTC TGGCATCAAG 
GGGGAGAAGG GTCATATTGG GTTAATTGGC CTCATTGGTC CCCCTGGAGA GCCTGGTGAG 
AAGGGAGACC AAGGAGTCCC AGGGGTACAA GGCACTCCAG GCCCTAAAGG AGAACCTGGT 
CTCCGAGGCC CTATAGGCCC TCTAGGACCC CGTGGGTCCC CAGGATTGGC GGGTCCGTTG 
GGGCAGAAAG GATCAAAAGG GTCACCAGGA TCCATTGGCC CCCGGGGAGA CACAGGAGCC 
CCAGGACCGC CCGGACCCCC GGGCTCCCCA GCAGAGGCGG GGGAACTCCT GCCCCTGGAG 
GGGTCCCGGC AGCGGCGCTC AGCTGAGGGA GTCCCTGGGC CTAGTGAGAG TGGCCTGGAG 
GAGGTATACG CCTCCCTCAG CTCCCTGAAG AGCGAAATAG ATCAGCTGAG AAGTCCTCGG 
GGGACCCAGG AGAGCCCCGG CCTGGTGTGT AAGGAGCTTG GCCGGAGCCA TCCCCACTTG 
CCTGATGGGG AATATTGGAT TGATCCCAAC CAGGGATGCT CAAGAGATGC TTTTAGAGTT 
TTCTGTAACT TCACAGCAGG AGGAGAGACG TGCATCTTTC CTGATAAGAA GTTTGAAGCA 
GTAAAGCTGG CTGCCTGGTC TAGGGAGAAG CCGGGGAACT GGTATAGCAC ATTTAAAAGA 
GGGAAGAAGT TCTCCTATGT GGACTCAGAT GGCAACGCAG TCCGTGTTGT GCAGCTGACC 
TTCTTGAAAC TATTGAGTGC TAGTGCTCGA CAGAGCTTCA CCTACATCTG CCAGAATTCT 
GTGGCGTGGC TAGATGAGAG AGAGGGTGAC TACAGTCGCT CCCTCCGATT CCTGGGGGCT 
GATGGGGAAG AACTGTCCCA CAACCGTACC TCAGATGTCA TCCGGGCTCA CCACGATGGA 
TGCAGGCTCC GGAAGGGCCA CTCTCAGACA GTCCTGGAGC TCCGGTCGTC CAAGGTGGGC 
CAGCTGCCCC TCAAAGACGT GGCTGCGTCA GACTTCGGGC AGACAAACCA AAAGTTTGGA 
TTTGAGCTGG GCCCTGTCTG CTTCAGCAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012397374.1
CDS	170..5482
Translation	MRDGTGSRERGWSRSWADFFLFLTSLMQLPQGGIADPVDVLQELGIKEGKAQVPEVPGFCPQRFPEGDRAFWVGNSSQLSVPVAKLWPAGHFPENFSLLITLRGYSSAQAVLLSIYDERGVRQLGLGVGRPTQLLSDPPSPSLLPSAVDLMDGRWHRVAVSVDSWTVTLVVDCEPQPPVSWGRQRLTPISIAGFIMLGTQDSGEETFQGDVQDLMILQDAQAAFRACENLLPGCDTPTTSATPSTPVEPDTSPPQRKGKGRGKGKGRKGRKGKGKKKKKNKESEKPEVPPISPEGQTITHIAERETLTLLPESEPVAPTVPSNVTVIASTVAIDLNVTTYKDPGIESVSFEDTQELPEPREGRDEERGNYTMNSIYGPAERGPESQIYIFPNAGEKGQKGEPAVIEVGQQFEGYPGLPGPLGIVGPSGPPGPPGFPGDPGERGPAGRPGLPGVDGIRGLPGTVIMMPFHFGGESLKGPPSSFQQAQAQAVLQQTQLSMQGPPGPIGLTGRAGPLGLPGYPGLKGEPGIAGPQGPRGLQGLPGPSGKEGKQGRSGADGPRGLPGDTGPKGDRGFDGLPGLPGEKGQRGDVGHVGQPGPPGEDGKKGSEGPPGPRGQAGEPGPRGLIGARGSPGAPGQPGVVGIDGPPGAKGNVGIPGEPGPPGQQGNPGSQGLPGPQGPIGEPGDKGPPGKPGIPGLPGAEGPPGHPGHEGPTGEKGAQGPEGSIGPTGYPGQRGVKGTSGNRGLKGEKGEKGEDGFPGFKGDAGLKGDRGDPGPLGPRGEDGPEGLKGQAGLLGEEGPPGPAGEKGKLGIPGLPGYPGRQGPKGSIGFPGPLGLPGEKGKRGKAGQPGQEGERGPPGARGERGQNGPTGKPGPKGDVGHDGSAGFPGEKGLPGLKGALGFPGPKGPPGPAGKEGRPGHPGQRGELGFQGQTGPPGPAGVIGPQGKTGETGPLGERGHPGPPGPPGEQGLPGLLGREGAQGDPGPAGLPGKDGPPGPRGFPGPRGAPGDTGTTGLKGSQGPPGPIGANGPPGERGPVGPAGGIGLPGQGGGQGPLGPAGETGAAGERGPPGPTGKDGIPGLPGPLGPAGATGPAGEDGDKGEMGAPGHKGSKGDKGEPGPPGPTGIQGPAGHPGLPGADGERGRRGPPGLFGQKGDDGVRGFPGGIGPPGLQGMPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGSPGLPGGIGQPGAVGEKGEPGEVGDPGPPGKPGPVGPKGEFGEKGDSGPTGAAGPPGKKGPPGEDGAKGSLGPTGFPGDPGPPGDPGVAGKEGVQGVKGDHGDVGAPGPPGASGEPGPPGPPGKRGPVGRIGQEGREGEKGAKGQPGPDGPPGRTGPVGPRGPPGRLGAEGLPGIPGPSGEPGLLGPPGQLGPPGPLGPSGLPGLKGDSGIKGEKGHIGLIGLIGPPGEPGEKGDQGVPGVQGTPGPKGEPGLRGPIGPLGPRGSPGLAGPLGQKGSKGSPGSIGPRGDTGAPGPPGPPGSPAEAGELLPLEGSRQRRSAEGVPGPSESGLEEVYASLSSLKSEIDQLRSPRGTQESPGLVCKELGRSHPHLPDGEYWIDPNQGCSRDAFRVFCNFTAGGETCIFPDKKFEAVKLAAWSREKPGNWYSTFKRGKKFSYVDSDGNAVRVVQLTFLKLLSASARQSFTYICQNSVAWLDEREGDYSRSLRFLGADGEELSHNRTSDVIRAHHDGCRLRKGHSQTVLELRSSKVGQLPLKDVAASDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_012541920.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012541920.2

ORF Insert Sequence:

ATGAGGGATG GGACCGGTAG CCGAGAACGG GGCTGGTCCA GATCCTGGGC AGATTTTTTC 
CTGTTCCTCA CTTCCCTGAT GCAACTGCCC CAAGGGGGAA TTGCTGATCC TGTAGATGTG 
CTTCAAGAAT TGGGAATCAA GGAAGGCAAA GCCCAGGTCC CCGAAGTGCC TGGCTTCTGC 
CCTCAGAGGT TCCCAGAAGG AGATCGAGCA TTCTGGGTGG GGAACTCCAG CCAACTCAGT 
GTTCCTGTGG CGAAGCTTTG GCCAGCTGGT CACTTTCCAG AGAACTTTTC ACTGCTGATC 
ACCCTGAGGG GCTATTCTTC CGCTCAGGCA GTCCTCCTGT CCATTTATGA TGAAAGGGGA 
GTGAGGCAAC TGGGACTAGG AGTGGGGCGG CCAACCCAGC TCCTGTCTGA CCCACCCAGC 
CCCAGCCTGC TTCCTTCAGC AGTTGACCTC ATGGATGGCA GGTGGCACCG TGTGGCGGTC 
AGTGTGGACA GCTGGACAGT GACTCTGGTG GTTGACTGTG AGCCACAGCC CCCAGTGAGC 
TGGGGCCGGC AGAGATTGAC CCCTATCAGC ATAGCTGGGT TCATTATGCT GGGGACACAG 
GACTCTGGAG AAGAGACCTT TCAGGGGGAT GTCCAGGACC TTATGATCCT GCAAGATGCT 
CAGGCTGCCT TCAGGGCCTG TGAAAACTTG CTGCCAGGGT GTGATACGCC CACTACCTCA 
GCTACCCCAT CTACCCCAGT TGAGCCAGAT ACCTCTCCTC CCCAAAGGAA AGGGAAGGGG 
AGAGGGAAGG GAAAGGGCCG GAAAGGCCGA AAGGGCAAGG GCAAGAAGAA GAAGAAAAAC 
AAGGAGTCTG AGAAACCAGA AGTGCCTCCA ATTTCACCAG AGGGCCAGAC CATCACTCAC 
ATTGCTGAGA GAGAGACTCT AACTCTCCTC CCTGAGTCAG AGCCTGTGGC CCCAACTGTC 
CCCTCAAATG TCACAGTCAT CGCATCCACA GTGGCCATCG ATCTCAATGT CACCACTTAT 
AAGGACCCTG GAATTGAGTC AGTGTCTTTT GAAGATACAC AGGAGCTCCC TGAGCCAAGG 
GAGGGAAGAG ATGAAGAGAG GGGAAACTAC ACTATGAACT CCATCTATGG ACCGGCTGAG 
CGTGGACCAG AGTCCCAGAT CTATATATTT CCTAATGCTG GTGAAAAAGG TCAGAAAGGA 
GAGCCAGCAG TGATTGAAGT GGGCCAGCAG TTTGAAGGGT ACCCAGGACT TCCAGGGCCT 
TTGGGAATAG TTGGTCCTTC AGGTCCTCCT GGTCCACCTG GCTTCCCTGG AGACCCAGGG 
GAAAGGGGTC CTGCTGGTCG TCCTGGTCTC CCTGGAGTGG ATGGTATCCG AGGCCTCCCA 
GGGACTGTAA TAATGATGCC TTTCCACTTT GGGGGTGAGT CCCTCAAGGG TCCCCCAAGC 
TCCTTCCAAC AGGCACAGGC ACAGGCAGTG CTACAGCAAA CCCAGCTCTC CATGCAAGGC 
CCTCCTGGCC CTATCGGGCT TACAGGTCGA GCTGGACCCT TGGGTCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAACCAGG GATAGCAGGA CCACAGGGTC CTAGAGGTCT ACAGGGTTTA 
CCTGGGCCCT CTGGAAAAGA AGGAAAACAG GGTCGCTCTG GAGCAGATGG ACCAAGGGGT 
CTCCCAGGGG ATACTGGACC TAAGGGTGAC CGGGGTTTCG ATGGCCTGCC CGGGCTGCCA 
GGAGAGAAAG GCCAAAGGGG AGATGTTGGC CATGTTGGGC AGCCAGGGCC CCCTGGCGAG 
GATGGTAAGA AGGGCTCAGA GGGGCCTCCA GGGCCCAGGG GCCAAGCTGG GGAACCAGGA 
CCCCGGGGGC TTATTGGCGC AAGAGGCTCC CCAGGTGCCC CAGGACAACC AGGTGTGGTG 
GGGATAGATG GACCTCCGGG TGCCAAAGGA AATGTGGGCA TCCCAGGAGA GCCAGGTCCT 
CCAGGTCAGC AAGGAAACCC AGGGTCCCAG GGTCTCCCTG GACCACAGGG ACCAATTGGG 
GAACCAGGGG ACAAGGGGCC CCCAGGAAAG CCAGGAATTC CAGGTCTTCC AGGAGCTGAA 
GGCCCACCGG GTCACCCCGG CCATGAGGGC CCCACAGGCG AGAAAGGGGC CCAGGGTCCA 
GAAGGGTCCA TAGGCCCAAC AGGGTATCCC GGGCAGCGAG GAGTAAAGGG CACATCTGGA 
AACCGAGGCC TAAAGGGGGA GAAAGGCGAG AAGGGAGAAG ATGGCTTCCC AGGCTTTAAG 
GGTGACGCAG GTCTAAAAGG AGATCGAGGT GACCCTGGAC CTCTGGGCCC AAGGGGAGAG 
GATGGGCCCG AGGGGCTGAA GGGTCAGGCA GGATTGCTGG GTGAAGAGGG ACCCCCAGGT 
CCAGCTGGGG AGAAGGGCAA GCTTGGTATC CCAGGCCTCC CAGGTTATCC AGGCCGTCAG 
GGTCCTAAGG GATCCATTGG ATTTCCTGGG CCACTGGGTC TGCCTGGAGA GAAAGGGAAA 
CGGGGGAAGG CTGGTCAGCC AGGACAGGAA GGCGAACGGG GACCACCAGG AGCCCGTGGA 
GAGAGGGGAC AGAATGGTCC CACAGGAAAA CCAGGCCCCA AGGGAGATGT GGGCCATGAT 
GGTTCTGCAG GGTTTCCTGG AGAAAAGGGA TTGCCGGGTC TAAAGGGAGC CCTTGGATTT 
CCTGGGCCAA AAGGACCCCC CGGCCCTGCA GGGAAGGAAG GAAGACCTGG TCACCCTGGG 
CAGCGGGGAG AGTTGGGTTT CCAAGGACAG ACAGGTCCCC CAGGACCAGC TGGTGTCATT 
GGCCCACAGG GGAAGACTGG TGAAACAGGG CCACTCGGTG AAAGGGGCCA TCCAGGCCCT 
CCTGGGCCCC CTGGAGAACA AGGGCTTCCA GGACTTCTGG GAAGAGAAGG AGCTCAGGGG 
GACCCTGGAC CAGCTGGTCT CCCTGGGAAG GATGGCCCCC CTGGTCCCCG AGGCTTCCCA 
GGCCCTCGTG GAGCCCCTGG AGATACAGGA ACAACTGGCC TGAAGGGGTC CCAGGGCCCC 
CCTGGCCCTA TTGGTGCCAA TGGTCCCCCC GGAGAACGAG GTCCTGTGGG CCCTGCTGGG 
GGCATTGGGT TGCCTGGTCA AGGGGGTGGA CAAGGGCCTC TTGGTCCTGC TGGAGAGACA 
GGAGCAGCAG GGGAGCGTGG ACCCCCTGGT CCCACAGGGA AAGATGGAAT TCCAGGGCTT 
CCAGGCCCCT TGGGACCAGC TGGAGCTACA GGGCCGGCTG GGGAAGATGG AGACAAGGGG 
GAAATGGGTG CCCCAGGCCA CAAGGGAAGC AAAGGTGACA AAGGGGAGCC GGGTCCCCCT 
GGGCCAACAG GGATCCAAGG TCCTGCTGGA CACCCAGGAC TTCCAGGTGC GGATGGGGAA 
CGAGGCCGTC GGGGTCCACC AGGCCTCTTT GGGCAGAAAG GAGATGATGG TGTGAGGGGC 
TTCCCAGGAG GCATTGGTCC CCCTGGCCTA CAGGGGATGC CAGGACCACC TGGAGAGAAG 
GGAGAGGTCG GAGATGTGGG CTCCATGGGT CCTCATGGTG CTCCAGGTCC CCGAGGACCT 
CAGGGCCCTA GTGGGTCAGA GGGATCTCCA GGGCTGCCTG GAGGCATTGG TCAACCAGGG 
GCTGTTGGAG AAAAAGGGGA ACCTGGGGAA GTGGGGGATC CTGGTCCCCC AGGCAAGCCT 
GGCCCAGTGG GACCCAAGGG AGAGTTTGGT GAAAAGGGTG ACTCAGGACC AACAGGGGCT 
GCCGGGCCTC CAGGGAAGAA AGGACCTCCT GGTGAAGATG GAGCCAAAGG GAGTCTGGGT 
CCCACAGGAT TCCCCGGAGA CCCTGGTCCC CCTGGAGACC CTGGTGTTGC TGGCAAAGAA 
GGTGTCCAGG GAGTGAAAGG GGACCACGGG GATGTCGGGG CTCCGGGCCC TCCTGGTGCC 
TCTGGAGAAC CTGGGCCTCC AGGGCCTCCT GGGAAAAGGG GGCCTGTTGG CCGGATAGGT 
CAAGAGGGCA GAGAAGGAGA GAAGGGGGCC AAGGGTCAGC CTGGTCCTGA TGGCCCCCCT 
GGAAGGACAG GCCCAGTGGG TCCAAGGGGC CCTCCTGGCC GACTTGGGGC TGAGGGCCTC 
CCAGGGATTC CTGGACCCTC GGGTGAGCCA GGTCTTCTTG GCCCTCCTGG ACAACTGGGT 
CCACCTGGGC CCCTGGGACC CAGTGGTCTC CCTGGCCTTA AAGGAGATTC TGGCATCAAG 
GGGGAGAAGG GTCATATTGG GTTAATTGGC CTCATTGGTC CCCCTGGAGA GCCTGGTGAG 
AAGGGAGACC AAGGAGTCCC AGGGGTACAA GGCACTCCAG GCCCTAAAGG AGAACCTGGT 
CTCCGAGGCC CTATAGGCCC TCTAGGACCC CGTGGGTCCC CAGGATTGGC GGGTCCGTTG 
GGGCAGAAAG GATCAAAAGG GTCACCAGGA TCCATTGGCC CCCGGGGAGA CACAGGAGCC 
CCAGGACCGC CCGGACCCCC GGGCTCCCCA GCAGAGGCGG GGGAACTCCT GCCCCTGGAG 
GGGTCCCGGC AGCGGCGCTC AGCTGAGGGA GTCCCTGGGC CTAGTGAGAG TGGCCTGGAG 
GAGGTATACG CCTCCCTCAG CTCCCTGAAG AGCGAAATAG ATCAGCTGAG AAGTCCTCGG 
GGGACCCAGG AGAGCCCCGG CCTGGTGTGT AAGGAGCTTG GCCGGAGCCA TCCCCACTTG 
CCTGATGGGG AATATTGGAT TGATCCCAAC CAGGGATGCT CAAGAGATGC TTTTAGAGTT 
TTCTGTAACT TCACAGCAGG AGGAGAGACG TGCATCTTTC CTGATAAGAA GTTTGAAGCA 
GTAAAGCTGG CTGCCTGGTC TAGGGAGAAG CCGGGGAACT GGTATAGCAC ATTTAAAAGA 
GGGAAGAAGT TCTCCTATGT GGACTCAGAT GGCAACGCAG TCCGTGTTGT GCAGCTGACC 
TTCTTGAAAC TATTGAGTGC TAGTGCTCGA CAGAGCTTCA CCTACATCTG CCAGAATTCT 
GTGGCGTGGC TAGATGAGAG AGAGGGTGAC TACAGTCGCT CCCTCCGATT CCTGGGGGCT 
GATGGGGAAG AACTGTCCCA CAACCGTACC TCAGATGTCA TCCGGGCTCA CCACGATGGA 
TGCAGGCTCC GGAAGGGCCA CTCTCAGACA GTCCTGGAGC TCCGGTCGTC CAAGGTGGGC 
CAGCTGCCCC TCAAAGACGT GGCTGCGTCA GACTTCGGGC AGACAAACCA AAAGTTTGGA 
TTTGAGCTGG GCCCTGTCTG CTTCAGCAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_012541920.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012397374.1 Sarcophilus harrisii collagen, type V, alpha 3 (COL5A3), mRNA.

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CloneID	OSa122168
Clone ID Related Accession (Same CDS sequence)	XM_012541920.2 , XM_012541920.1
Accession Version	XM_012541920.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-3(V) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823414.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGATG GGACCGGTAG CCGAGAACGG GGCTGGTCCA GATCCTGGGC AGATTTTTTC 
CTGTTCCTCA CTTCCCTGAT GCAACTGCCC CAAGGGGGAA TTGCTGATCC TGTAGATGTG 
CTTCAAGAAT TGGGAATCAA GGAAGGCAAA GCCCAGGTCC CCGAAGTGCC TGGCTTCTGC 
CCTCAGAGGT TCCCAGAAGG AGATCGAGCA TTCTGGGTGG GGAACTCCAG CCAACTCAGT 
GTTCCTGTGG CGAAGCTTTG GCCAGCTGGT CACTTTCCAG AGAACTTTTC ACTGCTGATC 
ACCCTGAGGG GCTATTCTTC CGCTCAGGCA GTCCTCCTGT CCATTTATGA TGAAAGGGGA 
GTGAGGCAAC TGGGACTAGG AGTGGGGCGG CCAACCCAGC TCCTGTCTGA CCCACCCAGC 
CCCAGCCTGC TTCCTTCAGC AGTTGACCTC ATGGATGGCA GGTGGCACCG TGTGGCGGTC 
AGTGTGGACA GCTGGACAGT GACTCTGGTG GTTGACTGTG AGCCACAGCC CCCAGTGAGC 
TGGGGCCGGC AGAGATTGAC CCCTATCAGC ATAGCTGGGT TCATTATGCT GGGGACACAG 
GACTCTGGAG AAGAGACCTT TCAGGGGGAT GTCCAGGACC TTATGATCCT GCAAGATGCT 
CAGGCTGCCT TCAGGGCCTG TGAAAACTTG CTGCCAGGGT GTGATACGCC CACTACCTCA 
GCTACCCCAT CTACCCCAGT TGAGCCAGAT ACCTCTCCTC CCCAAAGGAA AGGGAAGGGG 
AGAGGGAAGG GAAAGGGCCG GAAAGGCCGA AAGGGCAAGG GCAAGAAGAA GAAGAAAAAC 
AAGGAGTCTG AGAAACCAGA AGTGCCTCCA ATTTCACCAG AGGGCCAGAC CATCACTCAC 
ATTGCTGAGA GAGAGACTCT AACTCTCCTC CCTGAGTCAG AGCCTGTGGC CCCAACTGTC 
CCCTCAAATG TCACAGTCAT CGCATCCACA GTGGCCATCG ATCTCAATGT CACCACTTAT 
AAGGACCCTG GAATTGAGTC AGTGTCTTTT GAAGATACAC AGGAGCTCCC TGAGCCAAGG 
GAGGGAAGAG ATGAAGAGAG GGGAAACTAC ACTATGAACT CCATCTATGG ACCGGCTGAG 
CGTGGACCAG AGTCCCAGAT CTATATATTT CCTAATGCTG GTGAAAAAGG TCAGAAAGGA 
GAGCCAGCAG TGATTGAAGT GGGCCAGCAG TTTGAAGGGT ACCCAGGACT TCCAGGGCCT 
TTGGGAATAG TTGGTCCTTC AGGTCCTCCT GGTCCACCTG GCTTCCCTGG AGACCCAGGG 
GAAAGGGGTC CTGCTGGTCG TCCTGGTCTC CCTGGAGTGG ATGGTATCCG AGGCCTCCCA 
GGGACTGTAA TAATGATGCC TTTCCACTTT GGGGGTGAGT CCCTCAAGGG TCCCCCAAGC 
TCCTTCCAAC AGGCACAGGC ACAGGCAGTG CTACAGCAAA CCCAGCTCTC CATGCAAGGC 
CCTCCTGGCC CTATCGGGCT TACAGGTCGA GCTGGACCCT TGGGTCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAACCAGG GATAGCAGGA CCACAGGGTC CTAGAGGTCT ACAGGGTTTA 
CCTGGGCCCT CTGGAAAAGA AGGAAAACAG GGTCGCTCTG GAGCAGATGG ACCAAGGGGT 
CTCCCAGGGG ATACTGGACC TAAGGGTGAC CGGGGTTTCG ATGGCCTGCC CGGGCTGCCA 
GGAGAGAAAG GCCAAAGGGG AGATGTTGGC CATGTTGGGC AGCCAGGGCC CCCTGGCGAG 
GATGGTAAGA AGGGCTCAGA GGGGCCTCCA GGGCCCAGGG GCCAAGCTGG GGAACCAGGA 
CCCCGGGGGC TTATTGGCGC AAGAGGCTCC CCAGGTGCCC CAGGACAACC AGGTGTGGTG 
GGGATAGATG GACCTCCGGG TGCCAAAGGA AATGTGGGCA TCCCAGGAGA GCCAGGTCCT 
CCAGGTCAGC AAGGAAACCC AGGGTCCCAG GGTCTCCCTG GACCACAGGG ACCAATTGGG 
GAACCAGGGG ACAAGGGGCC CCCAGGAAAG CCAGGAATTC CAGGTCTTCC AGGAGCTGAA 
GGCCCACCGG GTCACCCCGG CCATGAGGGC CCCACAGGCG AGAAAGGGGC CCAGGGTCCA 
GAAGGGTCCA TAGGCCCAAC AGGGTATCCC GGGCAGCGAG GAGTAAAGGG CACATCTGGA 
AACCGAGGCC TAAAGGGGGA GAAAGGCGAG AAGGGAGAAG ATGGCTTCCC AGGCTTTAAG 
GGTGACGCAG GTCTAAAAGG AGATCGAGGT GACCCTGGAC CTCTGGGCCC AAGGGGAGAG 
GATGGGCCCG AGGGGCTGAA GGGTCAGGCA GGATTGCTGG GTGAAGAGGG ACCCCCAGGT 
CCAGCTGGGG AGAAGGGCAA GCTTGGTATC CCAGGCCTCC CAGGTTATCC AGGCCGTCAG 
GGTCCTAAGG GATCCATTGG ATTTCCTGGG CCACTGGGTC TGCCTGGAGA GAAAGGGAAA 
CGGGGGAAGG CTGGTCAGCC AGGACAGGAA GGCGAACGGG GACCACCAGG AGCCCGTGGA 
GAGAGGGGAC AGAATGGTCC CACAGGAAAA CCAGGCCCCA AGGGAGATGT GGGCCATGAT 
GGTTCTGCAG GGTTTCCTGG AGAAAAGGGA TTGCCGGGTC TAAAGGGAGC CCTTGGATTT 
CCTGGGCCAA AAGGACCCCC CGGCCCTGCA GGGAAGGAAG GAAGACCTGG TCACCCTGGG 
CAGCGGGGAG AGTTGGGTTT CCAAGGACAG ACAGGTCCCC CAGGACCAGC TGGTGTCATT 
GGCCCACAGG GGAAGACTGG TGAAACAGGG CCACTCGGTG AAAGGGGCCA TCCAGGCCCT 
CCTGGGCCCC CTGGAGAACA AGGGCTTCCA GGACTTCTGG GAAGAGAAGG AGCTCAGGGG 
GACCCTGGAC CAGCTGGTCT CCCTGGGAAG GATGGCCCCC CTGGTCCCCG AGGCTTCCCA 
GGCCCTCGTG GAGCCCCTGG AGATACAGGA ACAACTGGCC TGAAGGGGTC CCAGGGCCCC 
CCTGGCCCTA TTGGTGCCAA TGGTCCCCCC GGAGAACGAG GTCCTGTGGG CCCTGCTGGG 
GGCATTGGGT TGCCTGGTCA AGGGGGTGGA CAAGGGCCTC TTGGTCCTGC TGGAGAGACA 
GGAGCAGCAG GGGAGCGTGG ACCCCCTGGT CCCACAGGGA AAGATGGAAT TCCAGGGCTT 
CCAGGCCCCT TGGGACCAGC TGGAGCTACA GGGCCGGCTG GGGAAGATGG AGACAAGGGG 
GAAATGGGTG CCCCAGGCCA CAAGGGAAGC AAAGGTGACA AAGGGGAGCC GGGTCCCCCT 
GGGCCAACAG GGATCCAAGG TCCTGCTGGA CACCCAGGAC TTCCAGGTGC GGATGGGGAA 
CGAGGCCGTC GGGGTCCACC AGGCCTCTTT GGGCAGAAAG GAGATGATGG TGTGAGGGGC 
TTCCCAGGAG GCATTGGTCC CCCTGGCCTA CAGGGGATGC CAGGACCACC TGGAGAGAAG 
GGAGAGGTCG GAGATGTGGG CTCCATGGGT CCTCATGGTG CTCCAGGTCC CCGAGGACCT 
CAGGGCCCTA GTGGGTCAGA GGGATCTCCA GGGCTGCCTG GAGGCATTGG TCAACCAGGG 
GCTGTTGGAG AAAAAGGGGA ACCTGGGGAA GTGGGGGATC CTGGTCCCCC AGGCAAGCCT 
GGCCCAGTGG GACCCAAGGG AGAGTTTGGT GAAAAGGGTG ACTCAGGACC AACAGGGGCT 
GCCGGGCCTC CAGGGAAGAA AGGACCTCCT GGTGAAGATG GAGCCAAAGG GAGTCTGGGT 
CCCACAGGAT TCCCCGGAGA CCCTGGTCCC CCTGGAGACC CTGGTGTTGC TGGCAAAGAA 
GGTGTCCAGG GAGTGAAAGG GGACCACGGG GATGTCGGGG CTCCGGGCCC TCCTGGTGCC 
TCTGGAGAAC CTGGGCCTCC AGGGCCTCCT GGGAAAAGGG GGCCTGTTGG CCGGATAGGT 
CAAGAGGGCA GAGAAGGAGA GAAGGGGGCC AAGGGTCAGC CTGGTCCTGA TGGCCCCCCT 
GGAAGGACAG GCCCAGTGGG TCCAAGGGGC CCTCCTGGCC GACTTGGGGC TGAGGGCCTC 
CCAGGGATTC CTGGACCCTC GGGTGAGCCA GGTCTTCTTG GCCCTCCTGG ACAACTGGGT 
CCACCTGGGC CCCTGGGACC CAGTGGTCTC CCTGGCCTTA AAGGAGATTC TGGCATCAAG 
GGGGAGAAGG GTCATATTGG GTTAATTGGC CTCATTGGTC CCCCTGGAGA GCCTGGTGAG 
AAGGGAGACC AAGGAGTCCC AGGGGTACAA GGCACTCCAG GCCCTAAAGG AGAACCTGGT 
CTCCGAGGCC CTATAGGCCC TCTAGGACCC CGTGGGTCCC CAGGATTGGC GGGTCCGTTG 
GGGCAGAAAG GATCAAAAGG GTCACCAGGA TCCATTGGCC CCCGGGGAGA CACAGGAGCC 
CCAGGACCGC CCGGACCCCC GGGCTCCCCA GCAGAGGCGG GGGAACTCCT GCCCCTGGAG 
GGGTCCCGGC AGCGGCGCTC AGCTGAGGGA GTCCCTGGGC CTAGTGAGAG TGGCCTGGAG 
GAGGTATACG CCTCCCTCAG CTCCCTGAAG AGCGAAATAG ATCAGCTGAG AAGTCCTCGG 
GGGACCCAGG AGAGCCCCGG CCTGGTGTGT AAGGAGCTTG GCCGGAGCCA TCCCCACTTG 
CCTGATGGGG AATATTGGAT TGATCCCAAC CAGGGATGCT CAAGAGATGC TTTTAGAGTT 
TTCTGTAACT TCACAGCAGG AGGAGAGACG TGCATCTTTC CTGATAAGAA GTTTGAAGCA 
GTAAAGCTGG CTGCCTGGTC TAGGGAGAAG CCGGGGAACT GGTATAGCAC ATTTAAAAGA 
GGGAAGAAGT TCTCCTATGT GGACTCAGAT GGCAACGCAG TCCGTGTTGT GCAGCTGACC 
TTCTTGAAAC TATTGAGTGC TAGTGCTCGA CAGAGCTTCA CCTACATCTG CCAGAATTCT 
GTGGCGTGGC TAGATGAGAG AGAGGGTGAC TACAGTCGCT CCCTCCGATT CCTGGGGGCT 
GATGGGGAAG AACTGTCCCA CAACCGTACC TCAGATGTCA TCCGGGCTCA CCACGATGGA 
TGCAGGCTCC GGAAGGGCCA CTCTCAGACA GTCCTGGAGC TCCGGTCGTC CAAGGTGGGC 
CAGCTGCCCC TCAAAGACGT GGCTGCGTCA GACTTCGGGC AGACAAACCA AAAGTTTGGA 
TTTGAGCTGG GCCCTGTCTG CTTCAGCAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012397374.1
CDS	1..5313
Translation	MRDGTGSRERGWSRSWADFFLFLTSLMQLPQGGIADPVDVLQELGIKEGKAQVPEVPGFCPQRFPEGDRAFWVGNSSQLSVPVAKLWPAGHFPENFSLLITLRGYSSAQAVLLSIYDERGVRQLGLGVGRPTQLLSDPPSPSLLPSAVDLMDGRWHRVAVSVDSWTVTLVVDCEPQPPVSWGRQRLTPISIAGFIMLGTQDSGEETFQGDVQDLMILQDAQAAFRACENLLPGCDTPTTSATPSTPVEPDTSPPQRKGKGRGKGKGRKGRKGKGKKKKKNKESEKPEVPPISPEGQTITHIAERETLTLLPESEPVAPTVPSNVTVIASTVAIDLNVTTYKDPGIESVSFEDTQELPEPREGRDEERGNYTMNSIYGPAERGPESQIYIFPNAGEKGQKGEPAVIEVGQQFEGYPGLPGPLGIVGPSGPPGPPGFPGDPGERGPAGRPGLPGVDGIRGLPGTVIMMPFHFGGESLKGPPSSFQQAQAQAVLQQTQLSMQGPPGPIGLTGRAGPLGLPGYPGLKGEPGIAGPQGPRGLQGLPGPSGKEGKQGRSGADGPRGLPGDTGPKGDRGFDGLPGLPGEKGQRGDVGHVGQPGPPGEDGKKGSEGPPGPRGQAGEPGPRGLIGARGSPGAPGQPGVVGIDGPPGAKGNVGIPGEPGPPGQQGNPGSQGLPGPQGPIGEPGDKGPPGKPGIPGLPGAEGPPGHPGHEGPTGEKGAQGPEGSIGPTGYPGQRGVKGTSGNRGLKGEKGEKGEDGFPGFKGDAGLKGDRGDPGPLGPRGEDGPEGLKGQAGLLGEEGPPGPAGEKGKLGIPGLPGYPGRQGPKGSIGFPGPLGLPGEKGKRGKAGQPGQEGERGPPGARGERGQNGPTGKPGPKGDVGHDGSAGFPGEKGLPGLKGALGFPGPKGPPGPAGKEGRPGHPGQRGELGFQGQTGPPGPAGVIGPQGKTGETGPLGERGHPGPPGPPGEQGLPGLLGREGAQGDPGPAGLPGKDGPPGPRGFPGPRGAPGDTGTTGLKGSQGPPGPIGANGPPGERGPVGPAGGIGLPGQGGGQGPLGPAGETGAAGERGPPGPTGKDGIPGLPGPLGPAGATGPAGEDGDKGEMGAPGHKGSKGDKGEPGPPGPTGIQGPAGHPGLPGADGERGRRGPPGLFGQKGDDGVRGFPGGIGPPGLQGMPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGSPGLPGGIGQPGAVGEKGEPGEVGDPGPPGKPGPVGPKGEFGEKGDSGPTGAAGPPGKKGPPGEDGAKGSLGPTGFPGDPGPPGDPGVAGKEGVQGVKGDHGDVGAPGPPGASGEPGPPGPPGKRGPVGRIGQEGREGEKGAKGQPGPDGPPGRTGPVGPRGPPGRLGAEGLPGIPGPSGEPGLLGPPGQLGPPGPLGPSGLPGLKGDSGIKGEKGHIGLIGLIGPPGEPGEKGDQGVPGVQGTPGPKGEPGLRGPIGPLGPRGSPGLAGPLGQKGSKGSPGSIGPRGDTGAPGPPGPPGSPAEAGELLPLEGSRQRRSAEGVPGPSESGLEEVYASLSSLKSEIDQLRSPRGTQESPGLVCKELGRSHPHLPDGEYWIDPNQGCSRDAFRVFCNFTAGGETCIFPDKKFEAVKLAAWSREKPGNWYSTFKRGKKFSYVDSDGNAVRVVQLTFLKLLSASARQSFTYICQNSVAWLDEREGDYSRSLRFLGADGEELSHNRTSDVIRAHHDGCRLRKGHSQTVLELRSSKVGQLPLKDVAASDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_012541920.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen, type V, alpha 3 (COL5A3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012541920.1

ORF Insert Sequence:

ATGAGGGATG GGACCGGTAG CCGAGAACGG GGCTGGTCCA GATCCTGGGC AGATTTTTTC 
CTGTTCCTCA CTTCCCTGAT GCAACTGCCC CAAGGGGGAA TTGCTGATCC TGTAGATGTG 
CTTCAAGAAT TGGGAATCAA GGAAGGCAAA GCCCAGGTCC CCGAAGTGCC TGGCTTCTGC 
CCTCAGAGGT TCCCAGAAGG AGATCGAGCA TTCTGGGTGG GGAACTCCAG CCAACTCAGT 
GTTCCTGTGG CGAAGCTTTG GCCAGCTGGT CACTTTCCAG AGAACTTTTC ACTGCTGATC 
ACCCTGAGGG GCTATTCTTC CGCTCAGGCA GTCCTCCTGT CCATTTATGA TGAAAGGGGA 
GTGAGGCAAC TGGGACTAGG AGTGGGGCGG CCAACCCAGC TCCTGTCTGA CCCACCCAGC 
CCCAGCCTGC TTCCTTCAGC AGTTGACCTC ATGGATGGCA GGTGGCACCG TGTGGCGGTC 
AGTGTGGACA GCTGGACAGT GACTCTGGTG GTTGACTGTG AGCCACAGCC CCCAGTGAGC 
TGGGGCCGGC AGAGATTGAC CCCTATCAGC ATAGCTGGGT TCATTATGCT GGGGACACAG 
GACTCTGGAG AAGAGACCTT TCAGGGGGAT GTCCAGGACC TTATGATCCT GCAAGATGCT 
CAGGCTGCCT TCAGGGCCTG TGAAAACTTG CTGCCAGGGT GTGATACGCC CACTACCTCA 
GCTACCCCAT CTACCCCAGT TGAGCCAGAT ACCTCTCCTC CCCAAAGGAA AGGGAAGGGG 
AGAGGGAAGG GAAAGGGCCG GAAAGGCCGA AAGGGCAAGG GCAAGAAGAA GAAGAAAAAC 
AAGGAGTCTG AGAAACCAGA AGTGCCTCCA ATTTCACCAG AGGGCCAGAC CATCACTCAC 
ATTGCTGAGA GAGAGACTCT AACTCTCCTC CCTGAGTCAG AGCCTGTGGC CCCAACTGTC 
CCCTCAAATG TCACAGTCAT CGCATCCACA GTGGCCATCG ATCTCAATGT CACCACTTAT 
AAGGACCCTG GAATTGAGTC AGTGTCTTTT GAAGATACAC AGGAGCTCCC TGAGCCAAGG 
GAGGGAAGAG ATGAAGAGAG GGGAAACTAC ACTATGAACT CCATCTATGG ACCGGCTGAG 
CGTGGACCAG AGTCCCAGAT CTATATATTT CCTAATGCTG GTGAAAAAGG TCAGAAAGGA 
GAGCCAGCAG TGATTGAAGT GGGCCAGCAG TTTGAAGGGT ACCCAGGACT TCCAGGGCCT 
TTGGGAATAG TTGGTCCTTC AGGTCCTCCT GGTCCACCTG GCTTCCCTGG AGACCCAGGG 
GAAAGGGGTC CTGCTGGTCG TCCTGGTCTC CCTGGAGTGG ATGGTATCCG AGGCCTCCCA 
GGGACTGTAA TAATGATGCC TTTCCACTTT GGGGGTGAGT CCCTCAAGGG TCCCCCAAGC 
TCCTTCCAAC AGGCACAGGC ACAGGCAGTG CTACAGCAAA CCCAGCTCTC CATGCAAGGC 
CCTCCTGGCC CTATCGGGCT TACAGGTCGA GCTGGACCCT TGGGTCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAACCAGG GATAGCAGGA CCACAGGGTC CTAGAGGTCT ACAGGGTTTA 
CCTGGGCCCT CTGGAAAAGA AGGAAAACAG GGTCGCTCTG GAGCAGATGG ACCAAGGGGT 
CTCCCAGGGG ATACTGGACC TAAGGGTGAC CGGGGTTTCG ATGGCCTGCC CGGGCTGCCA 
GGAGAGAAAG GCCAAAGGGG AGATGTTGGC CATGTTGGGC AGCCAGGGCC CCCTGGCGAG 
GATGGTAAGA AGGGCTCAGA GGGGCCTCCA GGGCCCAGGG GCCAAGCTGG GGAACCAGGA 
CCCCGGGGGC TTATTGGCGC AAGAGGCTCC CCAGGTGCCC CAGGACAACC AGGTGTGGTG 
GGGATAGATG GACCTCCGGG TGCCAAAGGA AATGTGGGCA TCCCAGGAGA GCCAGGTCCT 
CCAGGTCAGC AAGGAAACCC AGGGTCCCAG GGTCTCCCTG GACCACAGGG ACCAATTGGG 
GAACCAGGGG ACAAGGGGCC CCCAGGAAAG CCAGGAATTC CAGGTCTTCC AGGAGCTGAA 
GGCCCACCGG GTCACCCCGG CCATGAGGGC CCCACAGGCG AGAAAGGGGC CCAGGGTCCA 
GAAGGGTCCA TAGGCCCAAC AGGGTATCCC GGGCAGCGAG GAGTAAAGGG CACATCTGGA 
AACCGAGGCC TAAAGGGGGA GAAAGGCGAG AAGGGAGAAG ATGGCTTCCC AGGCTTTAAG 
GGTGACGCAG GTCTAAAAGG AGATCGAGGT GACCCTGGAC CTCTGGGCCC AAGGGGAGAG 
GATGGGCCCG AGGGGCTGAA GGGTCAGGCA GGATTGCTGG GTGAAGAGGG ACCCCCAGGT 
CCAGCTGGGG AGAAGGGCAA GCTTGGTATC CCAGGCCTCC CAGGTTATCC AGGCCGTCAG 
GGTCCTAAGG GATCCATTGG ATTTCCTGGG CCACTGGGTC TGCCTGGAGA GAAAGGGAAA 
CGGGGGAAGG CTGGTCAGCC AGGACAGGAA GGCGAACGGG GACCACCAGG AGCCCGTGGA 
GAGAGGGGAC AGAATGGTCC CACAGGAAAA CCAGGCCCCA AGGGAGATGT GGGCCATGAT 
GGTTCTGCAG GGTTTCCTGG AGAAAAGGGA TTGCCGGGTC TAAAGGGAGC CCTTGGATTT 
CCTGGGCCAA AAGGACCCCC CGGCCCTGCA GGGAAGGAAG GAAGACCTGG TCACCCTGGG 
CAGCGGGGAG AGTTGGGTTT CCAAGGACAG ACAGGTCCCC CAGGACCAGC TGGTGTCATT 
GGCCCACAGG GGAAGACTGG TGAAACAGGG CCACTCGGTG AAAGGGGCCA TCCAGGCCCT 
CCTGGGCCCC CTGGAGAACA AGGGCTTCCA GGACTTCTGG GAAGAGAAGG AGCTCAGGGG 
GACCCTGGAC CAGCTGGTCT CCCTGGGAAG GATGGCCCCC CTGGTCCCCG AGGCTTCCCA 
GGCCCTCGTG GAGCCCCTGG AGATACAGGA ACAACTGGCC TGAAGGGGTC CCAGGGCCCC 
CCTGGCCCTA TTGGTGCCAA TGGTCCCCCC GGAGAACGAG GTCCTGTGGG CCCTGCTGGG 
GGCATTGGGT TGCCTGGTCA AGGGGGTGGA CAAGGGCCTC TTGGTCCTGC TGGAGAGACA 
GGAGCAGCAG GGGAGCGTGG ACCCCCTGGT CCCACAGGGA AAGATGGAAT TCCAGGGCTT 
CCAGGCCCCT TGGGACCAGC TGGAGCTACA GGGCCGGCTG GGGAAGATGG AGACAAGGGG 
GAAATGGGTG CCCCAGGCCA CAAGGGAAGC AAAGGTGACA AAGGGGAGCC GGGTCCCCCT 
GGGCCAACAG GGATCCAAGG TCCTGCTGGA CACCCAGGAC TTCCAGGTGC GGATGGGGAA 
CGAGGCCGTC GGGGTCCACC AGGCCTCTTT GGGCAGAAAG GAGATGATGG TGTGAGGGGC 
TTCCCAGGAG GCATTGGTCC CCCTGGCCTA CAGGGGATGC CAGGACCACC TGGAGAGAAG 
GGAGAGGTCG GAGATGTGGG CTCCATGGGT CCTCATGGTG CTCCAGGTCC CCGAGGACCT 
CAGGGCCCTA GTGGGTCAGA GGGATCTCCA GGGCTGCCTG GAGGCATTGG TCAACCAGGG 
GCTGTTGGAG AAAAAGGGGA ACCTGGGGAA GTGGGGGATC CTGGTCCCCC AGGCAAGCCT 
GGCCCAGTGG GACCCAAGGG AGAGTTTGGT GAAAAGGGTG ACTCAGGACC AACAGGGGCT 
GCCGGGCCTC CAGGGAAGAA AGGACCTCCT GGTGAAGATG GAGCCAAAGG GAGTCTGGGT 
CCCACAGGAT TCCCCGGAGA CCCTGGTCCC CCTGGAGACC CTGGTGTTGC TGGCAAAGAA 
GGTGTCCAGG GAGTGAAAGG GGACCACGGG GATGTCGGGG CTCCGGGCCC TCCTGGTGCC 
TCTGGAGAAC CTGGGCCTCC AGGGCCTCCT GGGAAAAGGG GGCCTGTTGG CCGGATAGGT 
CAAGAGGGCA GAGAAGGAGA GAAGGGGGCC AAGGGTCAGC CTGGTCCTGA TGGCCCCCCT 
GGAAGGACAG GCCCAGTGGG TCCAAGGGGC CCTCCTGGCC GACTTGGGGC TGAGGGCCTC 
CCAGGGATTC CTGGACCCTC GGGTGAGCCA GGTCTTCTTG GCCCTCCTGG ACAACTGGGT 
CCACCTGGGC CCCTGGGACC CAGTGGTCTC CCTGGCCTTA AAGGAGATTC TGGCATCAAG 
GGGGAGAAGG GTCATATTGG GTTAATTGGC CTCATTGGTC CCCCTGGAGA GCCTGGTGAG 
AAGGGAGACC AAGGAGTCCC AGGGGTACAA GGCACTCCAG GCCCTAAAGG AGAACCTGGT 
CTCCGAGGCC CTATAGGCCC TCTAGGACCC CGTGGGTCCC CAGGATTGGC GGGTCCGTTG 
GGGCAGAAAG GATCAAAAGG GTCACCAGGA TCCATTGGCC CCCGGGGAGA CACAGGAGCC 
CCAGGACCGC CCGGACCCCC GGGCTCCCCA GCAGAGGCGG GGGAACTCCT GCCCCTGGAG 
GGGTCCCGGC AGCGGCGCTC AGCTGAGGGA GTCCCTGGGC CTAGTGAGAG TGGCCTGGAG 
GAGGTATACG CCTCCCTCAG CTCCCTGAAG AGCGAAATAG ATCAGCTGAG AAGTCCTCGG 
GGGACCCAGG AGAGCCCCGG CCTGGTGTGT AAGGAGCTTG GCCGGAGCCA TCCCCACTTG 
CCTGATGGGG AATATTGGAT TGATCCCAAC CAGGGATGCT CAAGAGATGC TTTTAGAGTT 
TTCTGTAACT TCACAGCAGG AGGAGAGACG TGCATCTTTC CTGATAAGAA GTTTGAAGCA 
GTAAAGCTGG CTGCCTGGTC TAGGGAGAAG CCGGGGAACT GGTATAGCAC ATTTAAAAGA 
GGGAAGAAGT TCTCCTATGT GGACTCAGAT GGCAACGCAG TCCGTGTTGT GCAGCTGACC 
TTCTTGAAAC TATTGAGTGC TAGTGCTCGA CAGAGCTTCA CCTACATCTG CCAGAATTCT 
GTGGCGTGGC TAGATGAGAG AGAGGGTGAC TACAGTCGCT CCCTCCGATT CCTGGGGGCT 
GATGGGGAAG AACTGTCCCA CAACCGTACC TCAGATGTCA TCCGGGCTCA CCACGATGGA 
TGCAGGCTCC GGAAGGGCCA CTCTCAGACA GTCCTGGAGC TCCGGTCGTC CAAGGTGGGC 
CAGCTGCCCC TCAAAGACGT GGCTGCGTCA GACTTCGGGC AGACAAACCA AAAGTTTGGA 
TTTGAGCTGG GCCCTGTCTG CTTCAGCAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_023496595.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352363.1 Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.

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CloneID	OSa158903
Clone ID Related Accession (Same CDS sequence)	XM_023496595.1
Accession Version	XM_023496595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-3(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823414.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTG AGCCAGATAC CTCTCCTCCC CAAAGGAAAG GGAAGGGGAG AGGGAAGGGA 
AAGGGCCGGA AAGGCCGAAA GGGCAAGGGC AAGAAGAAGA AGAAAAACAA GGAGTCTGAG 
AAACCAGAAG TGCCTCCAAT TTCACCAGAG GGCCAGACCA TCACTCACAT TGCTGAGAGA 
GAGACTCTAA CTCTCCTCCC TGAGTCAGAG CCTGTGGCCC CAACTGTCCC CTCAAATGTC 
ACAGTCATCG CATCCACAGT GGCCATCGAT CTCAATGTCA CCACTTATAA GGACCCTGGA 
ATTGAGTCAG TGTCTTTTGA AGATACACAG GAGCTCCCTG AGCCAAGGGA GGGAAGAGAT 
GAAGAGAGGG GAAACTACAC TATGAACTCC ATCTATGGAC CGGCTGAGCG TGGACCAGAG 
TCCCAGATCT ATATATTTCC TAATGCTGGT GAAAAAGGTC AGAAAGGAGA GCCAGCAGTG 
ATTGAAGTGG GCCAGCAGTT TGAAGGGTAC CCAGGACTTC CAGGGCCTTT GGGAATAGTT 
GGTCCTTCAG GTCCTCCTGG TCCACCTGGC TTCCCTGGAG ACCCAGGGGA AAGGGGTCCT 
GCTGGTCGTC CTGGTCTCCC TGGAGTGGAT GGTATCCGAG GCCTCCCAGG GACTGTAATA 
ATGATGCCTT TCCACTTTGG GGGTGAGTCC CTCAAGGGTC CCCCAAGCTC CTTCCAACAG 
GCACAGGCAC AGGCAGTGCT ACAGCAAACC CAGCTCTCCA TGCAAGGCCC TCCTGGCCCT 
ATCGGGCTTA CAGGTCGAGC TGGACCCTTG GGTCTCCCTG GGTATCCAGG TCTGAAAGGA 
GAACCAGGGA TAGCAGGACC ACAGGGTCCT AGAGGTCTAC AGGGTTTACC TGGGCCCTCT 
GGAAAAGAAG GAAAACAGGG TCGCTCTGGA GCAGATGGAC CAAGGGGTCT CCCAGGGGAT 
ACTGGACCTA AGGGTGACCG GGGTTTCGAT GGCCTGCCCG GGCTGCCAGG AGAGAAAGGC 
CAAAGGGGAG ATGTTGGCCA TGTTGGGCAG CCAGGGCCCC CTGGCGAGGA TGGTAAGAAG 
GGCTCAGAGG GGCCTCCAGG GCCCAGGGGC CAAGCTGGGG AACCAGGACC CCGGGGGCTT 
ATTGGCGCAA GAGGCTCCCC AGGTGCCCCA GGACAACCAG GTGTGGTGGG GATAGATGGA 
CCTCCGGGTG CCAAAGGAAA TGTGGGCATC CCAGGAGAGC CAGGTCCTCC AGGTCAGCAA 
GGAAACCCAG GGTCCCAGGG TCTCCCTGGA CCACAGGGAC CAATTGGGGA ACCAGGGGAC 
AAGGGGCCCC CAGGAAAGCC AGGAATTCCA GGTCTTCCAG GAGCTGAAGG CCCACCGGGT 
CACCCCGGCC ATGAGGGCCC CACAGGCGAG AAAGGGGCCC AGGGTCCAGA AGGGTCCATA 
GGCCCAACAG GGTATCCCGG GCAGCGAGGA GTAAAGGGCA CATCTGGAAA CCGAGGCCTA 
AAGGGGGAGA AAGGCGAGAA GGGAGAAGAT GGCTTCCCAG GCTTTAAGGG TGACGCAGGT 
CTAAAAGGAG ATCGAGGTGA CCCTGGACCT CTGGGCCCAA GGGGAGAGGA TGGGCCCGAG 
GGGCTGAAGG GTCAGGCAGG ATTGCTGGGT GAAGAGGGAC CCCCAGGTCC AGCTGGGGAG 
AAGGGCAAGC TTGGTATCCC AGGCCTCCCA GGTTATCCAG GCCGTCAGGG TCCTAAGGGA 
TCCATTGGAT TTCCTGGGCC ACTGGGTCTG CCTGGAGAGA AAGGGAAACG GGGGAAGGCT 
GGTCAGCCAG GACAGGAAGG CGAACGGGGA CCACCAGGAG CCCGTGGAGA GAGGGGACAG 
AATGGTCCCA CAGGAAAACC AGGCCCCAAG GGAGATGTGG GCCATGATGG TTCTGCAGGG 
TTTCCTGGAG AAAAGGGATT GCCGGGTCTA AAGGGAGCCC TTGGATTTCC TGGGCCAAAA 
GGACCCCCCG GCCCTGCAGG GAAGGAAGGA AGACCTGGTC ACCCTGGGCA GCGGGGAGAG 
TTGGGTTTCC AAGGACAGAC AGGTCCCCCA GGACCAGCTG GTGTCATTGG CCCACAGGGG 
AAGACTGGTG AAACAGGGCC ACTCGGTGAA AGGGGCCATC CAGGCCCTCC TGGGCCCCCT 
GGAGAACAAG GGCTTCCAGG ACTTCTGGGA AGAGAAGGAG CTCAGGGGGA CCCTGGACCA 
GCTGGTCTCC CTGGGAAGGA TGGCCCCCCT GGTCCCCGAG GCTTCCCAGG CCCTCGTGGA 
GCCCCTGGAG ATACAGGAAC AACTGGCCTG AAGGGGTCCC AGGGCCCCCC TGGCCCTATT 
GGTGCCAATG GTCCCCCCGG AGAACGAGGT CCTGTGGGCC CTGCTGGGGG CATTGGGTTG 
CCTGGTCAAG GGGGTGGACA AGGGCCTCTT GGTCCTGCTG GAGAGACAGG AGCAGCAGGG 
GAGCGTGGAC CCCCTGGTCC CACAGGGAAA GATGGAATTC CAGGGCTTCC AGGCCCCTTG 
GGACCAGCTG GAGCTACAGG GCCGGCTGGG GAAGATGGAG ACAAGGGGGA AATGGGTGCC 
CCAGGCCACA AGGGAAGCAA AGGTGACAAA GGGGAGCCGG GTCCCCCTGG GCCAACAGGG 
ATCCAAGGTC CTGCTGGACA CCCAGGACTT CCAGGTGCGG ATGGGGAACG AGGCCGTCGG 
GGTCCACCAG GCCTCTTTGG GCAGAAAGGA GATGATGGTG TGAGGGGCTT CCCAGGAGGC 
ATTGGTCCCC CTGGCCTACA GGGGATGCCA GGACCACCTG GAGAGAAGGG AGAGGTCGGA 
GATGTGGGCT CCATGGGTCC TCATGGTGCT CCAGGTCCCC GAGGACCTCA GGGCCCTAGT 
GGGTCAGAGG GATCTCCAGG GCTGCCTGGA GGCATTGGTC AACCAGGGGC TGTTGGAGAA 
AAAGGGGAAC CTGGGGAAGT GGGGGATCCT GGTCCCCCAG GCAAGCCTGG CCCAGTGGGA 
CCCAAGGGAG AGTTTGGTGA AAAGGGTGAC TCAGGACCAA CAGGGGCTGC CGGGCCTCCA 
GGGAAGAAAG GACCTCCTGG TGAAGATGGA GCCAAAGGGA GTCTGGGTCC CACAGGATTC 
CCCGGAGACC CTGGTCCCCC TGGAGACCCT GGTGTTGCTG GCAAAGAAGG TGTCCAGGGA 
GTGAAAGGGG ACCACGGGGA TGTCGGGGCT CCGGGCCCTC CTGGTGCCTC TGGAGAACCT 
GGGCCTCCAG GGCCTCCTGG GAAAAGGGGG CCTGTTGGCC GGATAGGTCA AGAGGGCAGA 
GAAGGAGAGA AGGGGGCCAA GGGTCAGCCT GGTCCTGATG GCCCCCCTGG AAGGACAGGC 
CCAGTGGGTC CAAGGGGCCC TCCTGGCCGA CTTGGGGCTG AGGGCCTCCC AGGGATTCCT 
GGACCCTCGG GTGAGCCAGG TCTTCTTGGC CCTCCTGGAC AACTGGGTCC ACCTGGGCCC 
CTGGGACCCA GTGGTCTCCC TGGCCTTAAA GGAGATTCTG GCATCAAGGG GGAGAAGGGT 
CATATTGGGT TAATTGGCCT CATTGGTCCC CCTGGAGAGC CTGGTGAGAA GGGAGACCAA 
GGAGTCCCAG GGGTACAAGG CACTCCAGGC CCTAAAGGAG AACCTGGTCT CCGAGGCCCT 
ATAGGCCCTC TAGGACCCCG TGGGTCCCCA GGATTGGCGG GTCCGTTGGG GCAGAAAGGA 
TCAAAAGGGT CACCAGGATC CATTGGCCCC CGGGGAGACA CAGGAGCCCC AGGACCGCCC 
GGACCCCCGG GCTCCCCAGC AGAGGCGGGG GAACTCCTGC CCCTGGAGGG GTCCCGGCAG 
CGGCGCTCAG CTGAGGGAGT CCCTGGGCCT AGTGAGAGTG GCCTGGAGGA GGTATACGCC 
TCCCTCAGCT CCCTGAAGAG CGAAATAGAT CAGCTGAGAA GTCCTCGGGG GACCCAGGAG 
AGCCCCGGCC TGGTGTGTAA GGAGCTTGGC CGGAGCCATC CCCACTTGCC TGATGGGGAA 
TATTGGATTG ATCCCAACCA GGGATGCTCA AGAGATGCTT TTAGAGTTTT CTGTAACTTC 
ACAGCAGGAG GAGAGACGTG CATCTTTCCT GATAAGAAGT TTGAAGCAGT AAAGCTGGCT 
GCCTGGTCTA GGGAGAAGCC GGGGAACTGG TATAGCACAT TTAAAAGAGG GAAGAAGTTC 
TCCTATGTGG ACTCAGATGG CAACGCAGTC CGTGTTGTGC AGCTGACCTT CTTGAAACTA 
TTGAGTGCTA GTGCTCGACA GAGCTTCACC TACATCTGCC AGAATTCTGT GGCGTGGCTA 
GATGAGAGAG AGGGTGACTA CAGTCGCTCC CTCCGATTCC TGGGGGCTGA TGGGGAAGAA 
CTGTCCCACA ACCGTACCTC AGATGTCATC CGGGCTCACC ACGATGGATG CAGGCTCCGG 
AAGGGCCACT CTCAGACAGT CCTGGAGCTC CGGTCGTCCA AGGTGGGCCA GCTGCCCCTC 
AAAGACGTGG CTGCGTCAGA CTTCGGGCAG ACAAACCAAA AGTTTGGATT TGAGCTGGGC 
CCTGTCTGCT TCAGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352363.1
CDS	25..4605
Translation	MEVEPDTSPPQRKGKGRGKGKGRKGRKGKGKKKKKNKESEKPEVPPISPEGQTITHIAERETLTLLPESEPVAPTVPSNVTVIASTVAIDLNVTTYKDPGIESVSFEDTQELPEPREGRDEERGNYTMNSIYGPAERGPESQIYIFPNAGEKGQKGEPAVIEVGQQFEGYPGLPGPLGIVGPSGPPGPPGFPGDPGERGPAGRPGLPGVDGIRGLPGTVIMMPFHFGGESLKGPPSSFQQAQAQAVLQQTQLSMQGPPGPIGLTGRAGPLGLPGYPGLKGEPGIAGPQGPRGLQGLPGPSGKEGKQGRSGADGPRGLPGDTGPKGDRGFDGLPGLPGEKGQRGDVGHVGQPGPPGEDGKKGSEGPPGPRGQAGEPGPRGLIGARGSPGAPGQPGVVGIDGPPGAKGNVGIPGEPGPPGQQGNPGSQGLPGPQGPIGEPGDKGPPGKPGIPGLPGAEGPPGHPGHEGPTGEKGAQGPEGSIGPTGYPGQRGVKGTSGNRGLKGEKGEKGEDGFPGFKGDAGLKGDRGDPGPLGPRGEDGPEGLKGQAGLLGEEGPPGPAGEKGKLGIPGLPGYPGRQGPKGSIGFPGPLGLPGEKGKRGKAGQPGQEGERGPPGARGERGQNGPTGKPGPKGDVGHDGSAGFPGEKGLPGLKGALGFPGPKGPPGPAGKEGRPGHPGQRGELGFQGQTGPPGPAGVIGPQGKTGETGPLGERGHPGPPGPPGEQGLPGLLGREGAQGDPGPAGLPGKDGPPGPRGFPGPRGAPGDTGTTGLKGSQGPPGPIGANGPPGERGPVGPAGGIGLPGQGGGQGPLGPAGETGAAGERGPPGPTGKDGIPGLPGPLGPAGATGPAGEDGDKGEMGAPGHKGSKGDKGEPGPPGPTGIQGPAGHPGLPGADGERGRRGPPGLFGQKGDDGVRGFPGGIGPPGLQGMPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGSPGLPGGIGQPGAVGEKGEPGEVGDPGPPGKPGPVGPKGEFGEKGDSGPTGAAGPPGKKGPPGEDGAKGSLGPTGFPGDPGPPGDPGVAGKEGVQGVKGDHGDVGAPGPPGASGEPGPPGPPGKRGPVGRIGQEGREGEKGAKGQPGPDGPPGRTGPVGPRGPPGRLGAEGLPGIPGPSGEPGLLGPPGQLGPPGPLGPSGLPGLKGDSGIKGEKGHIGLIGLIGPPGEPGEKGDQGVPGVQGTPGPKGEPGLRGPIGPLGPRGSPGLAGPLGQKGSKGSPGSIGPRGDTGAPGPPGPPGSPAEAGELLPLEGSRQRRSAEGVPGPSESGLEEVYASLSSLKSEIDQLRSPRGTQESPGLVCKELGRSHPHLPDGEYWIDPNQGCSRDAFRVFCNFTAGGETCIFPDKKFEAVKLAAWSREKPGNWYSTFKRGKKFSYVDSDGNAVRVVQLTFLKLLSASARQSFTYICQNSVAWLDEREGDYSRSLRFLGADGEELSHNRTSDVIRAHHDGCRLRKGHSQTVLELRSSKVGQLPLKDVAASDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_023496595.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496595.1

ORF Insert Sequence:

ATGGAGGTTG AGCCAGATAC CTCTCCTCCC CAAAGGAAAG GGAAGGGGAG AGGGAAGGGA 
AAGGGCCGGA AAGGCCGAAA GGGCAAGGGC AAGAAGAAGA AGAAAAACAA GGAGTCTGAG 
AAACCAGAAG TGCCTCCAAT TTCACCAGAG GGCCAGACCA TCACTCACAT TGCTGAGAGA 
GAGACTCTAA CTCTCCTCCC TGAGTCAGAG CCTGTGGCCC CAACTGTCCC CTCAAATGTC 
ACAGTCATCG CATCCACAGT GGCCATCGAT CTCAATGTCA CCACTTATAA GGACCCTGGA 
ATTGAGTCAG TGTCTTTTGA AGATACACAG GAGCTCCCTG AGCCAAGGGA GGGAAGAGAT 
GAAGAGAGGG GAAACTACAC TATGAACTCC ATCTATGGAC CGGCTGAGCG TGGACCAGAG 
TCCCAGATCT ATATATTTCC TAATGCTGGT GAAAAAGGTC AGAAAGGAGA GCCAGCAGTG 
ATTGAAGTGG GCCAGCAGTT TGAAGGGTAC CCAGGACTTC CAGGGCCTTT GGGAATAGTT 
GGTCCTTCAG GTCCTCCTGG TCCACCTGGC TTCCCTGGAG ACCCAGGGGA AAGGGGTCCT 
GCTGGTCGTC CTGGTCTCCC TGGAGTGGAT GGTATCCGAG GCCTCCCAGG GACTGTAATA 
ATGATGCCTT TCCACTTTGG GGGTGAGTCC CTCAAGGGTC CCCCAAGCTC CTTCCAACAG 
GCACAGGCAC AGGCAGTGCT ACAGCAAACC CAGCTCTCCA TGCAAGGCCC TCCTGGCCCT 
ATCGGGCTTA CAGGTCGAGC TGGACCCTTG GGTCTCCCTG GGTATCCAGG TCTGAAAGGA 
GAACCAGGGA TAGCAGGACC ACAGGGTCCT AGAGGTCTAC AGGGTTTACC TGGGCCCTCT 
GGAAAAGAAG GAAAACAGGG TCGCTCTGGA GCAGATGGAC CAAGGGGTCT CCCAGGGGAT 
ACTGGACCTA AGGGTGACCG GGGTTTCGAT GGCCTGCCCG GGCTGCCAGG AGAGAAAGGC 
CAAAGGGGAG ATGTTGGCCA TGTTGGGCAG CCAGGGCCCC CTGGCGAGGA TGGTAAGAAG 
GGCTCAGAGG GGCCTCCAGG GCCCAGGGGC CAAGCTGGGG AACCAGGACC CCGGGGGCTT 
ATTGGCGCAA GAGGCTCCCC AGGTGCCCCA GGACAACCAG GTGTGGTGGG GATAGATGGA 
CCTCCGGGTG CCAAAGGAAA TGTGGGCATC CCAGGAGAGC CAGGTCCTCC AGGTCAGCAA 
GGAAACCCAG GGTCCCAGGG TCTCCCTGGA CCACAGGGAC CAATTGGGGA ACCAGGGGAC 
AAGGGGCCCC CAGGAAAGCC AGGAATTCCA GGTCTTCCAG GAGCTGAAGG CCCACCGGGT 
CACCCCGGCC ATGAGGGCCC CACAGGCGAG AAAGGGGCCC AGGGTCCAGA AGGGTCCATA 
GGCCCAACAG GGTATCCCGG GCAGCGAGGA GTAAAGGGCA CATCTGGAAA CCGAGGCCTA 
AAGGGGGAGA AAGGCGAGAA GGGAGAAGAT GGCTTCCCAG GCTTTAAGGG TGACGCAGGT 
CTAAAAGGAG ATCGAGGTGA CCCTGGACCT CTGGGCCCAA GGGGAGAGGA TGGGCCCGAG 
GGGCTGAAGG GTCAGGCAGG ATTGCTGGGT GAAGAGGGAC CCCCAGGTCC AGCTGGGGAG 
AAGGGCAAGC TTGGTATCCC AGGCCTCCCA GGTTATCCAG GCCGTCAGGG TCCTAAGGGA 
TCCATTGGAT TTCCTGGGCC ACTGGGTCTG CCTGGAGAGA AAGGGAAACG GGGGAAGGCT 
GGTCAGCCAG GACAGGAAGG CGAACGGGGA CCACCAGGAG CCCGTGGAGA GAGGGGACAG 
AATGGTCCCA CAGGAAAACC AGGCCCCAAG GGAGATGTGG GCCATGATGG TTCTGCAGGG 
TTTCCTGGAG AAAAGGGATT GCCGGGTCTA AAGGGAGCCC TTGGATTTCC TGGGCCAAAA 
GGACCCCCCG GCCCTGCAGG GAAGGAAGGA AGACCTGGTC ACCCTGGGCA GCGGGGAGAG 
TTGGGTTTCC AAGGACAGAC AGGTCCCCCA GGACCAGCTG GTGTCATTGG CCCACAGGGG 
AAGACTGGTG AAACAGGGCC ACTCGGTGAA AGGGGCCATC CAGGCCCTCC TGGGCCCCCT 
GGAGAACAAG GGCTTCCAGG ACTTCTGGGA AGAGAAGGAG CTCAGGGGGA CCCTGGACCA 
GCTGGTCTCC CTGGGAAGGA TGGCCCCCCT GGTCCCCGAG GCTTCCCAGG CCCTCGTGGA 
GCCCCTGGAG ATACAGGAAC AACTGGCCTG AAGGGGTCCC AGGGCCCCCC TGGCCCTATT 
GGTGCCAATG GTCCCCCCGG AGAACGAGGT CCTGTGGGCC CTGCTGGGGG CATTGGGTTG 
CCTGGTCAAG GGGGTGGACA AGGGCCTCTT GGTCCTGCTG GAGAGACAGG AGCAGCAGGG 
GAGCGTGGAC CCCCTGGTCC CACAGGGAAA GATGGAATTC CAGGGCTTCC AGGCCCCTTG 
GGACCAGCTG GAGCTACAGG GCCGGCTGGG GAAGATGGAG ACAAGGGGGA AATGGGTGCC 
CCAGGCCACA AGGGAAGCAA AGGTGACAAA GGGGAGCCGG GTCCCCCTGG GCCAACAGGG 
ATCCAAGGTC CTGCTGGACA CCCAGGACTT CCAGGTGCGG ATGGGGAACG AGGCCGTCGG 
GGTCCACCAG GCCTCTTTGG GCAGAAAGGA GATGATGGTG TGAGGGGCTT CCCAGGAGGC 
ATTGGTCCCC CTGGCCTACA GGGGATGCCA GGACCACCTG GAGAGAAGGG AGAGGTCGGA 
GATGTGGGCT CCATGGGTCC TCATGGTGCT CCAGGTCCCC GAGGACCTCA GGGCCCTAGT 
GGGTCAGAGG GATCTCCAGG GCTGCCTGGA GGCATTGGTC AACCAGGGGC TGTTGGAGAA 
AAAGGGGAAC CTGGGGAAGT GGGGGATCCT GGTCCCCCAG GCAAGCCTGG CCCAGTGGGA 
CCCAAGGGAG AGTTTGGTGA AAAGGGTGAC TCAGGACCAA CAGGGGCTGC CGGGCCTCCA 
GGGAAGAAAG GACCTCCTGG TGAAGATGGA GCCAAAGGGA GTCTGGGTCC CACAGGATTC 
CCCGGAGACC CTGGTCCCCC TGGAGACCCT GGTGTTGCTG GCAAAGAAGG TGTCCAGGGA 
GTGAAAGGGG ACCACGGGGA TGTCGGGGCT CCGGGCCCTC CTGGTGCCTC TGGAGAACCT 
GGGCCTCCAG GGCCTCCTGG GAAAAGGGGG CCTGTTGGCC GGATAGGTCA AGAGGGCAGA 
GAAGGAGAGA AGGGGGCCAA GGGTCAGCCT GGTCCTGATG GCCCCCCTGG AAGGACAGGC 
CCAGTGGGTC CAAGGGGCCC TCCTGGCCGA CTTGGGGCTG AGGGCCTCCC AGGGATTCCT 
GGACCCTCGG GTGAGCCAGG TCTTCTTGGC CCTCCTGGAC AACTGGGTCC ACCTGGGCCC 
CTGGGACCCA GTGGTCTCCC TGGCCTTAAA GGAGATTCTG GCATCAAGGG GGAGAAGGGT 
CATATTGGGT TAATTGGCCT CATTGGTCCC CCTGGAGAGC CTGGTGAGAA GGGAGACCAA 
GGAGTCCCAG GGGTACAAGG CACTCCAGGC CCTAAAGGAG AACCTGGTCT CCGAGGCCCT 
ATAGGCCCTC TAGGACCCCG TGGGTCCCCA GGATTGGCGG GTCCGTTGGG GCAGAAAGGA 
TCAAAAGGGT CACCAGGATC CATTGGCCCC CGGGGAGACA CAGGAGCCCC AGGACCGCCC 
GGACCCCCGG GCTCCCCAGC AGAGGCGGGG GAACTCCTGC CCCTGGAGGG GTCCCGGCAG 
CGGCGCTCAG CTGAGGGAGT CCCTGGGCCT AGTGAGAGTG GCCTGGAGGA GGTATACGCC 
TCCCTCAGCT CCCTGAAGAG CGAAATAGAT CAGCTGAGAA GTCCTCGGGG GACCCAGGAG 
AGCCCCGGCC TGGTGTGTAA GGAGCTTGGC CGGAGCCATC CCCACTTGCC TGATGGGGAA 
TATTGGATTG ATCCCAACCA GGGATGCTCA AGAGATGCTT TTAGAGTTTT CTGTAACTTC 
ACAGCAGGAG GAGAGACGTG CATCTTTCCT GATAAGAAGT TTGAAGCAGT AAAGCTGGCT 
GCCTGGTCTA GGGAGAAGCC GGGGAACTGG TATAGCACAT TTAAAAGAGG GAAGAAGTTC 
TCCTATGTGG ACTCAGATGG CAACGCAGTC CGTGTTGTGC AGCTGACCTT CTTGAAACTA 
TTGAGTGCTA GTGCTCGACA GAGCTTCACC TACATCTGCC AGAATTCTGT GGCGTGGCTA 
GATGAGAGAG AGGGTGACTA CAGTCGCTCC CTCCGATTCC TGGGGGCTGA TGGGGAAGAA 
CTGTCCCACA ACCGTACCTC AGATGTCATC CGGGCTCACC ACGATGGATG CAGGCTCCGG 
AAGGGCCACT CTCAGACAGT CCTGGAGCTC CGGTCGTCCA AGGTGGGCCA GCTGCCCCTC 
AAAGACGTGG CTGCGTCAGA CTTCGGGCAG ACAAACCAAA AGTTTGGATT TGAGCTGGGC 
CCTGTCTGCT TCAGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_023496595.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352363.2 Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), mRNA.

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CloneID	OSa221996
Clone ID Related Accession (Same CDS sequence)	XM_023496595.2
Accession Version	XM_023496595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-3(V) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023496595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGATG GGACCGGTAG CCGAGAACGG GGCTGGTCCA GATCCTGGGC AGATTTTTTC 
CTGTTCCTCA CTTCCCTGAT GCAACTGCCC CAAGGGGGAA TTGCTGATCC TGTAGATGTG 
CTTCAAGAAT TGGGAATCAA GGAAGGCAAA GCCCAGGTCC CCGAAGTGCC TGGCTTCTGC 
CCTCAGAGGT TCCCAGAAGG AGATCGAGCA TTCTGGGTGG GGAACTCCAG CCAACTCAGT 
GTTCCTGTGG CGAAGCTTTG GCCAGCTGGT CACTTTCCAG AGAACTTTTC ACTGCTGATC 
ACCCTGAGGG GCTATTCTTC CGCTCAGGCA GTCCTCCTGT CCATTTATGA TGAAAGGGGA 
GTGAGGCAAC TGGGACTAGG AGTGGGGCGG CCAACCCAGC TCCTGTCTGA CCCACCCAGC 
CCCAGCCTGC TTCCTTCAGC AGTTGACCTC ATGGATGGCA GGTGGCACCG TGTGGCGGTC 
AGTGTGGACA GCTGGACAGT GACTCTGGTG GTTGACTGTG AGCCACAGCC CCCAGTGAGC 
TGGGGCCGGC AGAGATTGAC CCCTATCAGC ATAGCTGGGT TCATTATGCT GGGGACACAG 
GACTCTGGAG AAGAGACCTT TCAGGGGGAT GTCCAGGACC TTATGATCCT GCAAGATGCT 
CAGGCTGCCT TCAGGGCCTG TGAAAACTTG CTGCCAGGGT GTGATACGCC CACTACCTCA 
GCTACCCCAT CTACCCCAGC CATCCCAGCT ACCCCAGCTA CCTCAGCTAC CCCAGCTGTC 
CCGTCTATCC CAGCTACCCC AGCTACCTCA GCTACTCCAG CCACTGCAGC TATCCCAGCC 
ATTCTGGCTA CCCCAGCCAT GGAGGTTGAG CCAGATACCT CTCCTCCCCA AAGGAAAGGG 
AAGGGGAGAG GGAAGGGAAA GGGCCGGAAA GGCCGAAAGG GCAAGGGCAA GAAGAAGAAG 
AAAAACAAGG AGTCTGAGAA ACCAGAAGTG CCTCCAATTT CACCAGAGGG CCAGACCATC 
ACTCACATTG CTGAGAGAGA GACTCTAACT CTCCTCCCTG AGTCAGAGCC TGTGGCCCCA 
ACTGTCCCCT CAAATGTCAC AGTCATCGCA TCCACAGTGG CCATCGATCT CAATGTCACC 
ACTTATAAGG ACCCTGGAAT TGAGTCAGTG TCTTTTGAAG ATACACAGGA GCTCCCTGAG 
CCAAGGGAGG GAAGAGATGA AGAGAGGGGA AACTACACTA TGAACTCCAT CTATGGACCG 
GCTGAGCGTG GACCAGAGTC CCAGATCTAT ATATTTCCTA ATGCTGGTGA AAAAGGTCAG 
AAAGGAGAGC CAGCAGTGAT TGAAGTGGGC CAGCAGTTTG AAGGGTACCC AGGACTTCCA 
GGGCCTTTGG GAATAGTTGG TCCTTCAGGT CCTCCTGGTC CACCTGGCTT CCCTGGAGAC 
CCAGGGGAAA GGGGTCCTGC TGGTCGTCCT GGTCTCCCTG GAGTGGATGG TATCCGAGGC 
CTCCCAGGGA CTGTAATAAT GATGCCTTTC CACTTTGGGG GTGAGTCCCT CAAGGGTCCC 
CCAAGCTCCT TCCAACAGGC ACAGGCACAG GCAGTGCTAC AGCAAACCCA GCTCTCCATG 
CAAGGCCCTC CTGGCCCTAT CGGGCTTACA GGTCGAGCTG GACCCTTGGG TCTCCCTGGG 
TATCCAGGTC TGAAAGGAGA ACCAGGGATA GCAGGACCAC AGGGTCCTAG AGGTCTACAG 
GGTTTACCTG GGCCCTCTGG AAAAGAAGGA AAACAGGGTC GCTCTGGAGC AGATGGACCA 
AGGGGTCTCC CAGGGGATAC TGGACCTAAG GGTGACCGGG GTTTCGATGG CCTGCCCGGG 
CTGCCAGGAG AGAAAGGCCA AAGGGGAGAT GTTGGCCATG TTGGGCAGCC AGGGCCCCCT 
GGCGAGGATG GTAAGAAGGG CTCAGAGGGG CCTCCAGGGC CCAGGGGCCA AGCTGGGGAA 
CCAGGACCCC GGGGGCTTAT TGGCGCAAGA GGCTCCCCAG GTGCCCCAGG ACAACCAGGT 
GTGGTGGGGA TAGATGGACC TCCGGGTGCC AAAGGAAATG TGGGCATCCC AGGAGAGCCA 
GGTCCTCCAG GTCAGCAAGG AAACCCAGGG TCCCAGGGTC TCCCTGGACC ACAGGGACCA 
ATTGGGGAAC CAGGGGACAA GGGGCCCCCA GGAAAGCCAG GAATTCCAGG TCTTCCAGGA 
GCTGAAGGCC CACCGGGTCA CCCCGGCCAT GAGGGCCCCA CAGGCGAGAA AGGGGCCCAG 
GGTCCAGAAG GGTCCATAGG CCCAACAGGG TATCCCGGGC AGCGAGGAGT AAAGGGCACA 
TCTGGAAACC GAGGCCTAAA GGGGGAGAAA GGCGAGAAGG GAGAAGATGG CTTCCCAGGC 
TTTAAGGGTG ACGCAGGTCT AAAAGGAGAT CGAGGTGACC CTGGACCTCT GGGCCCAAGG 
GGAGAGGATG GGCCCGAGGG GCTGAAGGGT CAGGCAGGAT TGCTGGGTGA AGAGGGACCC 
CCAGGTCCAG CTGGGGAGAA GGGCAAGCTT GGTATCCCAG GCCTCCCAGG TTATCCAGGC 
CGTCAGGGTC CTAAGGGATC CATTGGATTT CCTGGGCCAC TGGGTCTGCC TGGAGAGAAA 
GGGAAACGGG GGAAGGCTGG TCAGCCAGGA CAGGAAGGCG AACGGGGACC ACCAGGAGCC 
CGTGGAGAGA GGGGACAGAA TGGTCCCACA GGAAAACCAG GCCCCAAGGG AGATGTGGGC 
CATGATGGTT CTGCAGGGTT TCCTGGAGAA AAGGGATTGC CGGGTCTAAA GGGAGCCCTT 
GGATTTCCTG GGCCAAAAGG ACCCCCCGGC CCTGCAGGGA AGGAAGGAAG ACCTGGTCAC 
CCTGGGCAGC GGGGAGAGTT GGGTTTCCAA GGACAGACAG GTCCCCCAGG ACCAGCTGGT 
GTCATTGGCC CACAGGGGAA GACTGGTGAA ACAGGGCCAC TCGGTGAAAG GGGCCATCCA 
GGCCCTCCTG GGCCCCCTGG AGAACAAGGG CTTCCAGGAC TTCTGGGAAG AGAAGGAGCT 
CAGGGGGACC CTGGACCAGC TGGTCTCCCT GGGAAGGATG GCCCCCCTGG TCCCCGAGGC 
TTCCCAGGCC CTCGTGGAGC CCCTGGAGAT ACAGGAACAA CTGGCCTGAA GGGGTCCCAG 
GGCCCCCCTG GCCCTATTGG TGCCAATGGT CCCCCCGGAG AACGAGGTCC TGTGGGCCCT 
GCTGGGGGCA TTGGGTTGCC TGGTCAAGGG GGTGGACAAG GGCCTCTTGG TCCTGCTGGA 
GAGACAGGAG CAGCAGGGGA GCGTGGACCC CCTGGTCCCA CAGGGAAAGA TGGAATTCCA 
GGGCTTCCAG GCCCCTTGGG ACCAGCTGGA GCTACAGGGC CGGCTGGGGA AGATGGAGAC 
AAGGGGGAAA TGGGTGCCCC AGGCCACAAG GGAAGCAAAG GTGACAAAGG GGAGCCGGGT 
CCCCCTGGGC CAACAGGGAT CCAAGGTCCT GCTGGACACC CAGGACTTCC AGGTGCGGAT 
GGGGAACGAG GCCGTCGGGG TCCACCAGGC CTCTTTGGGC AGAAAGGAGA TGATGGTGTG 
AGGGGCTTCC CAGGAGGCAT TGGTCCCCCT GGCCTACAGG GGATGCCAGG ACCACCTGGA 
GAGAAGGGAG AGGTCGGAGA TGTGGGCTCC ATGGGTCCTC ATGGTGCTCC AGGTCCCCGA 
GGACCTCAGG GCCCTAGTGG GTCAGAGGGA TCTCCAGGGC TGCCTGGAGG CATTGGTCAA 
CCAGGGGCTG TTGGAGAAAA AGGGGAACCT GGGGAAGTGG GGGATCCTGG TCCCCCAGGC 
AAGCCTGGCC CAGTGGGACC CAAGGGAGAG TTTGGTGAAA AGGGTGACTC AGGACCAACA 
GGGGCTGCCG GGCCTCCAGG GAAGAAAGGA CCTCCTGGTG AAGATGGAGC CAAAGGGAGT 
CTGGGTCCCA CAGGATTCCC CGGAGACCCT GGTCCCCCTG GAGACCCTGG TGTTGCTGGC 
AAAGAAGGTG TCCAGGGAGT GAAAGGGGAC CACGGGGATG TCGGGGCTCC GGGCCCTCCT 
GGTGCCTCTG GAGAACCTGG GCCTCCAGGG CCTCCTGGGA AAAGGGGGCC TGTTGGCCGG 
ATAGGTCAAG AGGGCAGAGA AGGAGAGAAG GGGGCCAAGG GTCAGCCTGG TCCTGATGGC 
CCCCCTGGAA GGACAGGCCC AGTGGGTCCA AGGGGCCCTC CTGGCCGACT TGGGGCTGAG 
GGCCTCCCAG GGATTCCTGG ACCCTCGGGT GAGCCAGGTC TTCTTGGCCC TCCTGGACAA 
CTGGGTCCAC CTGGGCCCCT GGGACCCAGT GGTCTCCCTG GCCTTAAAGG AGATTCTGGC 
ATCAAGGGGG AGAAGGGTCA TATTGGGTTA ATTGGCCTCA TTGGTCCCCC TGGAGAGCCT 
GGTGAGAAGG GAGACCAAGG AGTCCCAGGG GTACAAGGCA CTCCAGGCCC TAAAGGAGAA 
CCTGGTCTCC GAGGCCCTAT AGGCCCTCTA GGACCCCGTG GGTCCCCAGG ATTGGCGGGT 
CCGTTGGGGC AGAAAGGATC AAAAGGGTCA CCAGGATCCA TTGGCCCCCG GGGAGACACA 
GGAGCCCCAG GACCGCCCGG ACCCCCGGGC TCCCCAGCAG AGGCGGGGGA ACTCCTGCCC 
CTGGAGGGGT CCCGGCAGCG GCGCTCAGCT GAGGGAGTCC CTGGGCCTAG TGAGAGTGGC 
CTGGAGGAGG TATACGCCTC CCTCAGCTCC CTGAAGAGCG AAATAGATCA GCTGAGAAGT 
CCTCGGGGGA CCCAGGAGAG CCCCGGCCTG GTGTGTAAGG AGCTTGGCCG GAGCCATCCC 
CACTTGCCTG ATGGGGAATA TTGGATTGAT CCCAACCAGG GATGCTCAAG AGATGCTTTT 
AGAGTTTTCT GTAACTTCAC AGCAGGAGGA GAGACGTGCA TCTTTCCTGA TAAGAAGTTT 
GAAGCAGTAA AGCTGGCTGC CTGGTCTAGG GAGAAGCCGG GGAACTGGTA TAGCACATTT 
AAAAGAGGGA AGAAGTTCTC CTATGTGGAC TCAGATGGCA ACGCAGTCCG TGTTGTGCAG 
CTGACCTTCT TGAAACTATT GAGTGCTAGT GCTCGACAGA GCTTCACCTA CATCTGCCAG 
AATTCTGTGG CGTGGCTAGA TGAGAGAGAG GGTGACTACA GTCGCTCCCT CCGATTCCTG 
GGGGCTGATG GGGAAGAACT GTCCCACAAC CGTACCTCAG ATGTCATCCG GGCTCACCAC 
GATGGATGCA GGCTCCGGAA GGGCCACTCT CAGACAGTCC TGGAGCTCCG GTCGTCCAAG 
GTGGGCCAGC TGCCCCTCAA AGACGTGGCT GCGTCAGACT TCGGGCAGAC AAACCAAAAG 
TTTGGATTTG AGCTGGGCCC TGTCTGCTTC AGCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352363.2
CDS	246..5684
Translation	MRDGTGSRERGWSRSWADFFLFLTSLMQLPQGGIADPVDVLQELGIKEGKAQVPEVPGFCPQRFPEGDRAFWVGNSSQLSVPVAKLWPAGHFPENFSLLITLRGYSSAQAVLLSIYDERGVRQLGLGVGRPTQLLSDPPSPSLLPSAVDLMDGRWHRVAVSVDSWTVTLVVDCEPQPPVSWGRQRLTPISIAGFIMLGTQDSGEETFQGDVQDLMILQDAQAAFRACENLLPGCDTPTTSATPSTPAIPATPATSATPAVPSIPATPATSATPATAAIPAILATPAMEVEPDTSPPQRKGKGRGKGKGRKGRKGKGKKKKKNKESEKPEVPPISPEGQTITHIAERETLTLLPESEPVAPTVPSNVTVIASTVAIDLNVTTYKDPGIESVSFEDTQELPEPREGRDEERGNYTMNSIYGPAERGPESQIYIFPNAGEKGQKGEPAVIEVGQQFEGYPGLPGPLGIVGPSGPPGPPGFPGDPGERGPAGRPGLPGVDGIRGLPGTVIMMPFHFGGESLKGPPSSFQQAQAQAVLQQTQLSMQGPPGPIGLTGRAGPLGLPGYPGLKGEPGIAGPQGPRGLQGLPGPSGKEGKQGRSGADGPRGLPGDTGPKGDRGFDGLPGLPGEKGQRGDVGHVGQPGPPGEDGKKGSEGPPGPRGQAGEPGPRGLIGARGSPGAPGQPGVVGIDGPPGAKGNVGIPGEPGPPGQQGNPGSQGLPGPQGPIGEPGDKGPPGKPGIPGLPGAEGPPGHPGHEGPTGEKGAQGPEGSIGPTGYPGQRGVKGTSGNRGLKGEKGEKGEDGFPGFKGDAGLKGDRGDPGPLGPRGEDGPEGLKGQAGLLGEEGPPGPAGEKGKLGIPGLPGYPGRQGPKGSIGFPGPLGLPGEKGKRGKAGQPGQEGERGPPGARGERGQNGPTGKPGPKGDVGHDGSAGFPGEKGLPGLKGALGFPGPKGPPGPAGKEGRPGHPGQRGELGFQGQTGPPGPAGVIGPQGKTGETGPLGERGHPGPPGPPGEQGLPGLLGREGAQGDPGPAGLPGKDGPPGPRGFPGPRGAPGDTGTTGLKGSQGPPGPIGANGPPGERGPVGPAGGIGLPGQGGGQGPLGPAGETGAAGERGPPGPTGKDGIPGLPGPLGPAGATGPAGEDGDKGEMGAPGHKGSKGDKGEPGPPGPTGIQGPAGHPGLPGADGERGRRGPPGLFGQKGDDGVRGFPGGIGPPGLQGMPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGSPGLPGGIGQPGAVGEKGEPGEVGDPGPPGKPGPVGPKGEFGEKGDSGPTGAAGPPGKKGPPGEDGAKGSLGPTGFPGDPGPPGDPGVAGKEGVQGVKGDHGDVGAPGPPGASGEPGPPGPPGKRGPVGRIGQEGREGEKGAKGQPGPDGPPGRTGPVGPRGPPGRLGAEGLPGIPGPSGEPGLLGPPGQLGPPGPLGPSGLPGLKGDSGIKGEKGHIGLIGLIGPPGEPGEKGDQGVPGVQGTPGPKGEPGLRGPIGPLGPRGSPGLAGPLGQKGSKGSPGSIGPRGDTGAPGPPGPPGSPAEAGELLPLEGSRQRRSAEGVPGPSESGLEEVYASLSSLKSEIDQLRSPRGTQESPGLVCKELGRSHPHLPDGEYWIDPNQGCSRDAFRVFCNFTAGGETCIFPDKKFEAVKLAAWSREKPGNWYSTFKRGKKFSYVDSDGNAVRVVQLTFLKLLSASARQSFTYICQNSVAWLDEREGDYSRSLRFLGADGEELSHNRTSDVIRAHHDGCRLRKGHSQTVLELRSSKVGQLPLKDVAASDFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_023496595.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type V alpha 3 chain (COL5A3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496595.2

ORF Insert Sequence:

ATGAGGGATG GGACCGGTAG CCGAGAACGG GGCTGGTCCA GATCCTGGGC AGATTTTTTC 
CTGTTCCTCA CTTCCCTGAT GCAACTGCCC CAAGGGGGAA TTGCTGATCC TGTAGATGTG 
CTTCAAGAAT TGGGAATCAA GGAAGGCAAA GCCCAGGTCC CCGAAGTGCC TGGCTTCTGC 
CCTCAGAGGT TCCCAGAAGG AGATCGAGCA TTCTGGGTGG GGAACTCCAG CCAACTCAGT 
GTTCCTGTGG CGAAGCTTTG GCCAGCTGGT CACTTTCCAG AGAACTTTTC ACTGCTGATC 
ACCCTGAGGG GCTATTCTTC CGCTCAGGCA GTCCTCCTGT CCATTTATGA TGAAAGGGGA 
GTGAGGCAAC TGGGACTAGG AGTGGGGCGG CCAACCCAGC TCCTGTCTGA CCCACCCAGC 
CCCAGCCTGC TTCCTTCAGC AGTTGACCTC ATGGATGGCA GGTGGCACCG TGTGGCGGTC 
AGTGTGGACA GCTGGACAGT GACTCTGGTG GTTGACTGTG AGCCACAGCC CCCAGTGAGC 
TGGGGCCGGC AGAGATTGAC CCCTATCAGC ATAGCTGGGT TCATTATGCT GGGGACACAG 
GACTCTGGAG AAGAGACCTT TCAGGGGGAT GTCCAGGACC TTATGATCCT GCAAGATGCT 
CAGGCTGCCT TCAGGGCCTG TGAAAACTTG CTGCCAGGGT GTGATACGCC CACTACCTCA 
GCTACCCCAT CTACCCCAGC CATCCCAGCT ACCCCAGCTA CCTCAGCTAC CCCAGCTGTC 
CCGTCTATCC CAGCTACCCC AGCTACCTCA GCTACTCCAG CCACTGCAGC TATCCCAGCC 
ATTCTGGCTA CCCCAGCCAT GGAGGTTGAG CCAGATACCT CTCCTCCCCA AAGGAAAGGG 
AAGGGGAGAG GGAAGGGAAA GGGCCGGAAA GGCCGAAAGG GCAAGGGCAA GAAGAAGAAG 
AAAAACAAGG AGTCTGAGAA ACCAGAAGTG CCTCCAATTT CACCAGAGGG CCAGACCATC 
ACTCACATTG CTGAGAGAGA GACTCTAACT CTCCTCCCTG AGTCAGAGCC TGTGGCCCCA 
ACTGTCCCCT CAAATGTCAC AGTCATCGCA TCCACAGTGG CCATCGATCT CAATGTCACC 
ACTTATAAGG ACCCTGGAAT TGAGTCAGTG TCTTTTGAAG ATACACAGGA GCTCCCTGAG 
CCAAGGGAGG GAAGAGATGA AGAGAGGGGA AACTACACTA TGAACTCCAT CTATGGACCG 
GCTGAGCGTG GACCAGAGTC CCAGATCTAT ATATTTCCTA ATGCTGGTGA AAAAGGTCAG 
AAAGGAGAGC CAGCAGTGAT TGAAGTGGGC CAGCAGTTTG AAGGGTACCC AGGACTTCCA 
GGGCCTTTGG GAATAGTTGG TCCTTCAGGT CCTCCTGGTC CACCTGGCTT CCCTGGAGAC 
CCAGGGGAAA GGGGTCCTGC TGGTCGTCCT GGTCTCCCTG GAGTGGATGG TATCCGAGGC 
CTCCCAGGGA CTGTAATAAT GATGCCTTTC CACTTTGGGG GTGAGTCCCT CAAGGGTCCC 
CCAAGCTCCT TCCAACAGGC ACAGGCACAG GCAGTGCTAC AGCAAACCCA GCTCTCCATG 
CAAGGCCCTC CTGGCCCTAT CGGGCTTACA GGTCGAGCTG GACCCTTGGG TCTCCCTGGG 
TATCCAGGTC TGAAAGGAGA ACCAGGGATA GCAGGACCAC AGGGTCCTAG AGGTCTACAG 
GGTTTACCTG GGCCCTCTGG AAAAGAAGGA AAACAGGGTC GCTCTGGAGC AGATGGACCA 
AGGGGTCTCC CAGGGGATAC TGGACCTAAG GGTGACCGGG GTTTCGATGG CCTGCCCGGG 
CTGCCAGGAG AGAAAGGCCA AAGGGGAGAT GTTGGCCATG TTGGGCAGCC AGGGCCCCCT 
GGCGAGGATG GTAAGAAGGG CTCAGAGGGG CCTCCAGGGC CCAGGGGCCA AGCTGGGGAA 
CCAGGACCCC GGGGGCTTAT TGGCGCAAGA GGCTCCCCAG GTGCCCCAGG ACAACCAGGT 
GTGGTGGGGA TAGATGGACC TCCGGGTGCC AAAGGAAATG TGGGCATCCC AGGAGAGCCA 
GGTCCTCCAG GTCAGCAAGG AAACCCAGGG TCCCAGGGTC TCCCTGGACC ACAGGGACCA 
ATTGGGGAAC CAGGGGACAA GGGGCCCCCA GGAAAGCCAG GAATTCCAGG TCTTCCAGGA 
GCTGAAGGCC CACCGGGTCA CCCCGGCCAT GAGGGCCCCA CAGGCGAGAA AGGGGCCCAG 
GGTCCAGAAG GGTCCATAGG CCCAACAGGG TATCCCGGGC AGCGAGGAGT AAAGGGCACA 
TCTGGAAACC GAGGCCTAAA GGGGGAGAAA GGCGAGAAGG GAGAAGATGG CTTCCCAGGC 
TTTAAGGGTG ACGCAGGTCT AAAAGGAGAT CGAGGTGACC CTGGACCTCT GGGCCCAAGG 
GGAGAGGATG GGCCCGAGGG GCTGAAGGGT CAGGCAGGAT TGCTGGGTGA AGAGGGACCC 
CCAGGTCCAG CTGGGGAGAA GGGCAAGCTT GGTATCCCAG GCCTCCCAGG TTATCCAGGC 
CGTCAGGGTC CTAAGGGATC CATTGGATTT CCTGGGCCAC TGGGTCTGCC TGGAGAGAAA 
GGGAAACGGG GGAAGGCTGG TCAGCCAGGA CAGGAAGGCG AACGGGGACC ACCAGGAGCC 
CGTGGAGAGA GGGGACAGAA TGGTCCCACA GGAAAACCAG GCCCCAAGGG AGATGTGGGC 
CATGATGGTT CTGCAGGGTT TCCTGGAGAA AAGGGATTGC CGGGTCTAAA GGGAGCCCTT 
GGATTTCCTG GGCCAAAAGG ACCCCCCGGC CCTGCAGGGA AGGAAGGAAG ACCTGGTCAC 
CCTGGGCAGC GGGGAGAGTT GGGTTTCCAA GGACAGACAG GTCCCCCAGG ACCAGCTGGT 
GTCATTGGCC CACAGGGGAA GACTGGTGAA ACAGGGCCAC TCGGTGAAAG GGGCCATCCA 
GGCCCTCCTG GGCCCCCTGG AGAACAAGGG CTTCCAGGAC TTCTGGGAAG AGAAGGAGCT 
CAGGGGGACC CTGGACCAGC TGGTCTCCCT GGGAAGGATG GCCCCCCTGG TCCCCGAGGC 
TTCCCAGGCC CTCGTGGAGC CCCTGGAGAT ACAGGAACAA CTGGCCTGAA GGGGTCCCAG 
GGCCCCCCTG GCCCTATTGG TGCCAATGGT CCCCCCGGAG AACGAGGTCC TGTGGGCCCT 
GCTGGGGGCA TTGGGTTGCC TGGTCAAGGG GGTGGACAAG GGCCTCTTGG TCCTGCTGGA 
GAGACAGGAG CAGCAGGGGA GCGTGGACCC CCTGGTCCCA CAGGGAAAGA TGGAATTCCA 
GGGCTTCCAG GCCCCTTGGG ACCAGCTGGA GCTACAGGGC CGGCTGGGGA AGATGGAGAC 
AAGGGGGAAA TGGGTGCCCC AGGCCACAAG GGAAGCAAAG GTGACAAAGG GGAGCCGGGT 
CCCCCTGGGC CAACAGGGAT CCAAGGTCCT GCTGGACACC CAGGACTTCC AGGTGCGGAT 
GGGGAACGAG GCCGTCGGGG TCCACCAGGC CTCTTTGGGC AGAAAGGAGA TGATGGTGTG 
AGGGGCTTCC CAGGAGGCAT TGGTCCCCCT GGCCTACAGG GGATGCCAGG ACCACCTGGA 
GAGAAGGGAG AGGTCGGAGA TGTGGGCTCC ATGGGTCCTC ATGGTGCTCC AGGTCCCCGA 
GGACCTCAGG GCCCTAGTGG GTCAGAGGGA TCTCCAGGGC TGCCTGGAGG CATTGGTCAA 
CCAGGGGCTG TTGGAGAAAA AGGGGAACCT GGGGAAGTGG GGGATCCTGG TCCCCCAGGC 
AAGCCTGGCC CAGTGGGACC CAAGGGAGAG TTTGGTGAAA AGGGTGACTC AGGACCAACA 
GGGGCTGCCG GGCCTCCAGG GAAGAAAGGA CCTCCTGGTG AAGATGGAGC CAAAGGGAGT 
CTGGGTCCCA CAGGATTCCC CGGAGACCCT GGTCCCCCTG GAGACCCTGG TGTTGCTGGC 
AAAGAAGGTG TCCAGGGAGT GAAAGGGGAC CACGGGGATG TCGGGGCTCC GGGCCCTCCT 
GGTGCCTCTG GAGAACCTGG GCCTCCAGGG CCTCCTGGGA AAAGGGGGCC TGTTGGCCGG 
ATAGGTCAAG AGGGCAGAGA AGGAGAGAAG GGGGCCAAGG GTCAGCCTGG TCCTGATGGC 
CCCCCTGGAA GGACAGGCCC AGTGGGTCCA AGGGGCCCTC CTGGCCGACT TGGGGCTGAG 
GGCCTCCCAG GGATTCCTGG ACCCTCGGGT GAGCCAGGTC TTCTTGGCCC TCCTGGACAA 
CTGGGTCCAC CTGGGCCCCT GGGACCCAGT GGTCTCCCTG GCCTTAAAGG AGATTCTGGC 
ATCAAGGGGG AGAAGGGTCA TATTGGGTTA ATTGGCCTCA TTGGTCCCCC TGGAGAGCCT 
GGTGAGAAGG GAGACCAAGG AGTCCCAGGG GTACAAGGCA CTCCAGGCCC TAAAGGAGAA 
CCTGGTCTCC GAGGCCCTAT AGGCCCTCTA GGACCCCGTG GGTCCCCAGG ATTGGCGGGT 
CCGTTGGGGC AGAAAGGATC AAAAGGGTCA CCAGGATCCA TTGGCCCCCG GGGAGACACA 
GGAGCCCCAG GACCGCCCGG ACCCCCGGGC TCCCCAGCAG AGGCGGGGGA ACTCCTGCCC 
CTGGAGGGGT CCCGGCAGCG GCGCTCAGCT GAGGGAGTCC CTGGGCCTAG TGAGAGTGGC 
CTGGAGGAGG TATACGCCTC CCTCAGCTCC CTGAAGAGCG AAATAGATCA GCTGAGAAGT 
CCTCGGGGGA CCCAGGAGAG CCCCGGCCTG GTGTGTAAGG AGCTTGGCCG GAGCCATCCC 
CACTTGCCTG ATGGGGAATA TTGGATTGAT CCCAACCAGG GATGCTCAAG AGATGCTTTT 
AGAGTTTTCT GTAACTTCAC AGCAGGAGGA GAGACGTGCA TCTTTCCTGA TAAGAAGTTT 
GAAGCAGTAA AGCTGGCTGC CTGGTCTAGG GAGAAGCCGG GGAACTGGTA TAGCACATTT 
AAAAGAGGGA AGAAGTTCTC CTATGTGGAC TCAGATGGCA ACGCAGTCCG TGTTGTGCAG 
CTGACCTTCT TGAAACTATT GAGTGCTAGT GCTCGACAGA GCTTCACCTA CATCTGCCAG 
AATTCTGTGG CGTGGCTAGA TGAGAGAGAG GGTGACTACA GTCGCTCCCT CCGATTCCTG 
GGGGCTGATG GGGAAGAACT GTCCCACAAC CGTACCTCAG ATGTCATCCG GGCTCACCAC 
GATGGATGCA GGCTCCGGAA GGGCCACTCT CAGACAGTCC TGGAGCTCCG GTCGTCCAAG 
GTGGGCCAGC TGCCCCTCAA AGACGTGGCT GCGTCAGACT TCGGGCAGAC AAACCAAAAG 
TTTGGATTTG AGCTGGGCCC TGTCTGCTTC AGCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL5A3 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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