Cbs cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cbs
Entrez Gene ID	24250
Full Name	cystathionine beta synthase
General protein information	Preferred Names cystathionine beta synthase Names cystathionine beta-synthase beta-thionase hemoprotein H-450 serine sulfhydrase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	catalyzes condensation of serine and homocyteine to cystathione; involved in transsulfuration pathway; regulated by insulin; alternative splicing produces four mRNA species [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012522.2	NP_036654.2	cystathionine beta-synthase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0149700	NP_001056819.1
Neurospora crassa	NCU08216	XP_959306.1
Xenopus tropicalis (tropical clawed frog)	cbs	NP_001008171.1
Homo sapiens (human)	LOC102724560	XP_006723989.1
Magnaporthe oryzae (rice blast fungus)	MGG_07384	XP_003711260.1
Eremothecium gossypii	AGOS_AGR012C	NP_986677.2
Homo sapiens (human)	CBS	NP_000062.1
Bos taurus (cattle)	CBS	NP_001095470.1
Mus musculus (house mouse)	Cbs	NP_001258282.1
Rattus norvegicus (Norway rat)	Cbs	NP_036654.2
Gallus gallus (chicken)	CBS	XP_416752.3
Drosophila melanogaster (fruit fly)	Cbs	NP_728384.1
Kluyveromyces lactis	KLLA0F09317g	XP_455504.1
Canis lupus familiaris (dog)	CBS	XP_853796.2
Danio rerio (zebrafish)	cbsb	NP_001014367.2
Saccharomyces cerevisiae (baker's yeast)	CYS4	NP_011671.3
Oryza sativa (rice)	Os06g0564500	NP_001057884.1
Oryza sativa (rice)	Os06g0564700	NP_001057885.1
Oryza sativa (rice)	Os06g0149900	NP_001056820.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000162	XP_310977.5
Pan troglodytes (chimpanzee)	CBS	XP_001143121.1
Danio rerio (zebrafish)	cbsa	NP_001104702.1

Gene Ontology
Bibliography

Related articles in PubMed

Hyperhomocysteinemia in polycystic ovary syndrome: decreased betaine-homocysteine methyltransferase and cystathionine β-synthase-mediated homocysteine metabolism.
Li D, Liu HX, Fang YY, Huo JN, Wu QJ, Wang TR, Zhou YM, Wang XX, Ma XX
Reproductive biomedicine online37(2)234-241(2018 08)

Prenatal maternal stress induces visceral hypersensitivity of adult rat offspring through activation of cystathionine-β-synthase signaling in primary sensory neurons.
Wang HJ, Xu X, Xie RH, Rui YY, Zhang PA, Zhu XJ, Xu GY
Molecular pain141744806918777406(2018 Jan-Dec)

Nitration-mediated deficiency of cystathionine β-synthase activity accelerates the progression of hyperhomocysteinemia.
Wang H, Sun Q, Zhou Y, Zhang H, Luo C, Xu J, Dong Y, Wu Y, Liu H, Wang W
Free radical biology & medicine113519-529(2017 12)

Excessive nNOS/NO/AMPK signaling activation mediated by the blockage of the CBS/H2S system contributes to oxygen‑glucose deprivation‑induced endoplasmic reticulum stress in PC12 cells.
Zhang R, Lin YQ, Wang WS, Wang XQ
International journal of molecular medicine40(2)549-557(2017 Aug)

Loss of the Protein Cystathionine β-Synthase During Kidney Injury Promotes Renal Tubulointerstitial Fibrosis.
Yuan X, Zhang J, Xie F, Tan W, Wang S, Huang L, Tao L, Xing Q, Yuan Q
Kidney & blood pressure research42(3)428-443(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cbs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cbs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12746	NM_012522.2 Latest version!	Rattus norvegicus cystathionine beta synthase (Cbs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cbs ( NM_012522.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036654.2 Rattus norvegicus cystathionine beta synthase (Cbs), mRNA.

Price & Availability↑

CloneID	ORa12746
Clone ID Related Accession (Same CDS sequence)	NM_012522.2
Accession Version	NM_012522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	cystathionine beta-synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M88344.1, D01098.1, M88346.1, M88345.1 and AA997754.1. On Oct 12, 2007 this sequence version replaced NM_012522.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D01098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCTTCAG GGACATCCCA GTGTGAAGAT GGCTCTGCAG GGTGCCCCCA GGACTTGGAG 
GTACAGCCAG AAAAAGGGCA ACTGGAGAAG GGAGCCTCAG GGGACAAGGA AAGAGTCTGG 
ATCTCGCCTG ATACCCCAAG CAGATGTACT TGGCAGCTGG GCAGGCCCAT GGCGGATTCC 
CCACATTACC ACACAGTGCC GACAAAATCC CCGAAAATTT TGCCAGATAT TCTGAGGAAA 
ATTGGCAACA CCCCTATGGT CAGAATCAAC AGGATCTCCA AGAATGCAGG ACTCAAGTGC 
GAGCTGTTGG CCAAGTGTGA GTTCTTCAAC GCCGGTGGGA GTGTGAAGGA CCGCATCAGC 
CTCCGGATGA TTGAAGACGC TGAGCGAGCC GGAACCTTGA AGCCCGGAGA CACGATCATT 
GAGCCAACTT CTGGCAACAC AGGGATCGGG CTGGCTCTGG CAGCTGCTGT GAAGGGCTAT 
CGCTGCATTA TCGTGATGCC TGAGAAGATG AGTATGGAGA AGGTGGATGT GCTGCGAGCT 
CTGGGAGCTG AGATTGTGAG GACGCCCACC AACGCCAGAT TCGATTCCCC CGAGTCCCAC 
GTAGGAGTGG CATGGCGACT GAAGAACGAA ATCCCCAATT CTCACATTCT GGACCAGTAC 
CGCAATGCCA GCAACCCCTT GGCGCACTAC GATGACACCG CAGAGGAGAT CCTGCAGCAG 
TGCGACGGGA AGGTGGACAT GCTGGTGGCT TCAGCAGGCA CGGGTGGCAC CATCACGGGT 
ATCGCGAGGA AGCTGAAGGA GAAGTGCCCA GGTTGTAAAA TCATCGGTGT AGATCCCGAG 
GGGTCCATCC TCGCGGAGCC CGAGGAGCTG AACCAGACGG AGCAAACAGC CTATGAGGTG 
GAAGGGATCG GCTACGACTT CATCCCCACC GTCCTGGACA GGGCGGTGGT GGATAGGTGG 
TTCAAGAGCA ATGATGACGA TTCCTTCGCC TTCGCCCGCA TGCTCATCTC CCAGGAGGGA 
CTGCTGTGCG GTGGGAGTTC AGGCAGCGCT ATGGCCGTGG CTGTGAAGGC TGCCCAGGAG 
CTAAAGGAAG GACAGCGCTG TGTGGTCATC CTGCCCGACT CTGTGCGCAA CTACATGTCC 
AAGTTCTTGA GTGACAAATG GATGCTGCAG AAAGGCTTCA TGAAGGAGGA GCTCTCCGTG 
AAGAGACCCT GGTGGTGGCA TCTGCGTGTC CAAGAGCTGA GCCTATCAGC ACCGCTGACC 
GTGTTGCCCA CTGTCACCTG TGAGCACACC ATCGCCATCC TCCGGGAGAA GGGTTTTGAC 
CAGGCACCTG TGGTCAACGA GTCTGGGGCC ATCCTAGGGA TGGTGACTCT CGGGAACATG 
TTGTCCTCCC TGCTTGCTGG GAAGGTGCGG CCATCAGACG AAGTCTGCAA AGTCCTCTAC 
AAGCAGTTCA AGCCGATCCA CCTGACCGAC ACACTGGGCA TGCTCTCCCA CATCCTGGAG 
ATGGACCACT TCGCCCTGGT GGTCCATGAG CAGATCCAAT ACCGCAACAA TGGCGTGTCC 
AGCAAGCAGC TGATGGTGTT TGGTGTTGTC ACCGCCATTG ACCTGCTAAA CTTCGTGGCA 
GCCCGTGAGC AGACCCGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036654.2
CDS	145..1788
Translation	MPSGTSQCEDGSAGCPQDLEVQPEKGQLEKGASGDKERVWISPDTPSRCTWQLGRPMADSPHYHTVPTKSPKILPDILRKIGNTPMVRINRISKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERAGTLKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDDTAEEILQQCDGKVDMLVASAGTGGTITGIARKLKEKCPGCKIIGVDPEGSILAEPEELNQTEQTAYEVEGIGYDFIPTVLDRAVVDRWFKSNDDDSFAFARMLISQEGLLCGGSSGSAMAVAVKAAQELKEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFMKEELSVKRPWWWHLRVQELSLSAPLTVLPTVTCEHTIAILREKGFDQAPVVNESGAILGMVTLGNMLSSLLAGKVRPSDEVCKVLYKQFKPIHLTDTLGMLSHILEMDHFALVVHEQIQYRNNGVSSKQLMVFGVVTAIDLLNFVAAREQTRK

Target ORF information:

RefSeq Version	NM_012522.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cystathionine beta synthase (Cbs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012522.2

ORF Insert Sequence:

ATGCCTTCAG GGACATCCCA GTGTGAAGAT GGCTCTGCAG GGTGCCCCCA GGACTTGGAG 
GTACAGCCAG AAAAAGGGCA ACTGGAGAAG GGAGCCTCAG GGGACAAGGA AAGAGTCTGG 
ATCTCGCCTG ATACCCCAAG CAGATGTACT TGGCAGCTGG GCAGGCCCAT GGCGGATTCC 
CCACATTACC ACACAGTGCC GACAAAATCC CCGAAAATTT TGCCAGATAT TCTGAGGAAA 
ATTGGCAACA CCCCTATGGT CAGAATCAAC AGGATCTCCA AGAATGCAGG ACTCAAGTGC 
GAGCTGTTGG CCAAGTGTGA GTTCTTCAAC GCCGGTGGGA GTGTGAAGGA CCGCATCAGC 
CTCCGGATGA TTGAAGACGC TGAGCGAGCC GGAACCTTGA AGCCCGGAGA CACGATCATT 
GAGCCAACTT CTGGCAACAC AGGGATCGGG CTGGCTCTGG CAGCTGCTGT GAAGGGCTAT 
CGCTGCATTA TCGTGATGCC TGAGAAGATG AGTATGGAGA AGGTGGATGT GCTGCGAGCT 
CTGGGAGCTG AGATTGTGAG GACGCCCACC AACGCCAGAT TCGATTCCCC CGAGTCCCAC 
GTAGGAGTGG CATGGCGACT GAAGAACGAA ATCCCCAATT CTCACATTCT GGACCAGTAC 
CGCAATGCCA GCAACCCCTT GGCGCACTAC GATGACACCG CAGAGGAGAT CCTGCAGCAG 
TGCGACGGGA AGGTGGACAT GCTGGTGGCT TCAGCAGGCA CGGGTGGCAC CATCACGGGT 
ATCGCGAGGA AGCTGAAGGA GAAGTGCCCA GGTTGTAAAA TCATCGGTGT AGATCCCGAG 
GGGTCCATCC TCGCGGAGCC CGAGGAGCTG AACCAGACGG AGCAAACAGC CTATGAGGTG 
GAAGGGATCG GCTACGACTT CATCCCCACC GTCCTGGACA GGGCGGTGGT GGATAGGTGG 
TTCAAGAGCA ATGATGACGA TTCCTTCGCC TTCGCCCGCA TGCTCATCTC CCAGGAGGGA 
CTGCTGTGCG GTGGGAGTTC AGGCAGCGCT ATGGCCGTGG CTGTGAAGGC TGCCCAGGAG 
CTAAAGGAAG GACAGCGCTG TGTGGTCATC CTGCCCGACT CTGTGCGCAA CTACATGTCC 
AAGTTCTTGA GTGACAAATG GATGCTGCAG AAAGGCTTCA TGAAGGAGGA GCTCTCCGTG 
AAGAGACCCT GGTGGTGGCA TCTGCGTGTC CAAGAGCTGA GCCTATCAGC ACCGCTGACC 
GTGTTGCCCA CTGTCACCTG TGAGCACACC ATCGCCATCC TCCGGGAGAA GGGTTTTGAC 
CAGGCACCTG TGGTCAACGA GTCTGGGGCC ATCCTAGGGA TGGTGACTCT CGGGAACATG 
TTGTCCTCCC TGCTTGCTGG GAAGGTGCGG CCATCAGACG AAGTCTGCAA AGTCCTCTAC 
AAGCAGTTCA AGCCGATCCA CCTGACCGAC ACACTGGGCA TGCTCTCCCA CATCCTGGAG 
ATGGACCACT TCGCCCTGGT GGTCCATGAG CAGATCCAAT ACCGCAACAA TGGCGTGTCC 
AGCAAGCAGC TGATGGTGTT TGGTGTTGTC ACCGCCATTG ACCTGCTAAA CTTCGTGGCA 
GCCCGTGAGC AGACCCGGAA ATAG