ANAPC1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANAPC1
Entrez Gene ID	105303965
Full Name	anaphase promoting complex subunit 1
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011377840.2	XP_011376142.1	anaphase-promoting complex subunit 1
XM_011377840.1	XP_011376142.1	anaphase-promoting complex subunit 1
XM_023521936.1	XP_023377704.1	anaphase-promoting complex subunit 1

Pathways from BioSystems
Gene Ontology

The following ANAPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANAPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt59533	XM_011377840.2 Latest version!	Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPt59533	XM_023521936.1 Latest version!	Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OPt59533	XM_011377840.1	Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANAPC1 ( XM_011377840.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011376142.1 Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), transcript variant X1, mRNA.

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CloneID	OPt59533
Clone ID Related Accession (Same CDS sequence)	XM_011377840.2 , XM_011377840.1 , XM_023521936.1
Accession Version	XM_011377840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	anaphase-promoting complex subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889104.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011377840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACT TCTATGAAGA AAGGGCAACA ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTCG GTCGAGACCA CTGCAAGCAT CACCCTAATG CTCTGAACCT TCAACTGCGC 
CAGCTGCAGC CGGCTTCTGA GTTATGGTCG TCCGATGGTG CTGCTGGCTT GGTGGGGTCC 
CTTCAGGAGG TTACAATCCA CGAGAAACAG AAGGAAAGCT GGCAGTTAAG GAAAGGCGTA 
AGCGAAATTG GAGAAGAAGT GGATTATGAT GAGGAACTCT ATGTGGCTGG AAATATGGTG 
ATCTGGAGCA AAGGGAGTAA AAGTCAGGCG CTGGCAGTTT ATAAAGCGTT TACAGTTGAC 
AGTCCTGTTC AACAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAA 
GCCTACAGTA GCGATGAAGT AGAGAAATGC ATATGTATAT TGCAAAGTTC GTGTATTAAC 
ATGCACAGCA TAGGAGGAAA GGATTACATA GCTTCTTTAC CTTTTCAGGT TGCCGGTGTC 
TGGCCTACTA AGTATGGACT GTTGTTCGAA CGGAGCACTT CTGCACACGA GGCACCTCCC 
GGCCCGCCCA GAGAACCTTT ACCCACCATG TTCAGCATGC TGCATCCACT GGATGAAATA 
ACCCCACTTG TGTGTAAATC GGGAAGCCTT TTCGGTTCAT CACGGGTGCA ATATGTTGTA 
GATCACGCGA TGAAAATAGT TTTTCTCAAC ATGGACCCCT CCATTGTAAT GACCTACGAC 
ACTGTTCAGA GTCTACATTC TGTGTGGGTT CTCCGGAGAG TTAAAACAGA GGAAGAGAAT 
GTTGTCTTAA AGTTCTCTGA ACAGGGGGGC ACCCCCCAGA ATGTGGCCAC GAGTAGCTCC 
CTCACAGCTC ACCTCAGAAG CCTTTCCAAA GGAGACTCTC CCGTGGGCTC GCCTTTCCAG 
AATTACTCCT CAGTCCACAG TCAGAGCCGC TCCACCTCAT CGCCCAGTCT GCACTCTCGG 
TCTCCTTCCA TCTCCAACAT GGCAGCTCTG AGCCGTGCTC ACTCCCCTGC CTCAGGAGCG 
CACTCTTTCT CAGGGGTGCA AAGGTTCAAC CTTTCAAGCC ACAATCAGTC TCCAAGGAGA 
CACAGCATTT CTCAGTCTCC AAATAGCCAT TCAAATGGCT CTTTCCTTGC GCCAGAAACC 
GAGCCGATTG TTCCTGAACT GTGTATTGAA CATGTATGGA CAGAAGCAAT TACCAGTATG 
AGAGAGAAAA ATTCACAGGC CTCCAAAGTG TTTATTACAT CTGACCTATG TGGGCAGAAG 
TTCTTGTGCT TCTTAGTAGA GTCCCAGCTC CAGTTACGCT GTGTGAAGTT TCAAGAGAGT 
AATGATAAGA CCCAGCTCAT CTTCGGCTCC ATCACCAACC TGCAGGCAAA GGATGCGGCG 
CCAGTGGAGA AGTTAGACAC CATGCTGGTC CTGGAAAGCA GTGGCAACCT GGTGCTATAC 
ACAGGCGTGG TCCGGGTGGG AAAAGTTTTT ATCCCTGGCC TGCCAGCCCC TTCCCTGACA 
GCGTCCCGTG CGGTGCCTCG TCCCAGCACC CCGCTAGACA GCATCAGCAC CCCTGAGCCT 
CTGAGTAAGC TGCTTGGATC CTTGGATGAG GCTGTTCTGT TGTCCCCAGT TCCAGAACTA 
AGAGATTCTT CGAAACTCCA TGATTCTCTC TATAATGAGG ATTGCACCTT CCAGCAGCTT 
GGGACTTATA TCCACTCTGT TAGAGACCCC GTCCGTAATA GAGTGACTCT CGAGCTGAGT 
AACGGCACCA TGGTTAGGAT CACTCTTCCT GAAATTGCCA CCTCTGAATT AGTACAAACA 
TGTTTGCAAG CCATTAAATT TATCCTGCCA AAAGAAATAG CTGTTCAGAT GCTTGTCAAG 
TGGTACAGTG TCCACAGCGC TCCGGGCAGC CCCAGCTATC ACTCAGAGTG GAATTTATTT 
GTGATTTGTC TCATGAACAT GATGGGTTAT AACACAGACC GCTTAGCGTG GACAAGAAAT 
TTTGACTTTG AAGGATCACT TTCTCCAGTC ATCGCACCCA AAAAGGCAAG GCCTTCTGAG 
ACGGGATCTG ATGAGGACTG GGAGTATTTG CTAAATTCAG ACTATCACCA GAGTGCCGAG 
TCTCACCTTC TGAGCAGGTC TCTCTGTCTG AGTCCTTTGG AGGCTCCTCA GGTGAAAGGA 
GAGGACTCAT CACAGAGCCT CAGTCTGGAC TCCTCCACGC TTCTCTTCTC CCACATGCCT 
GCAGTGTTTT TTGTTCTTCA CCTTGTCTAT GAGGAGCTCA AGCTGAACAC TCTGATGGGA 
GAAGGAAGTC GCTCACTTGT TGACCTGCTT GTTCAGTTGG CAAGAGACTT AAAATTGGAG 
CCTTACTTAG ATCATTACTA TAGAGATTAC CCGACGCTTG TCAGAACTAC TGGACAAGTG 
TGCACAGTTG ATCCAGGTCA AACAGGATTT ATGCATCACC CATCATTTTT CACTTCCGAG 
CCACCAAGTA TTTACCAGTG GGTGAGTTCT TGTCTGAAGG GCGAGGGGTC GCCACCCTAC 
CCCTACCTCC CTGGTGCCTG TGAAAGAAGC CGGCTCGTGG TCTTGAGTAT TGCACTCTAC 
ATTCTAGGTG ATGAAAGCTC TGTTTCTGAT GAATCCTCCT CACAGTATTT ATCCAGAATA 
ACTCCAACGT CCCAGAAGTC ACAAGCAGAA CAAGAGGAAA ACAGGCTTAG TGTCCAGCAT 
TCTACATCAG TTTCTAGTCT AGCCGAAAGA TTAGTTGTCT GGATGACTAG TGTAGGATTC 
ACTCTAAGGG ACTTGGAAAC GCTTCCCTTT GGAATTGCTC TTCCCATCAG AGATGCAATT 
TATCACTGTC GGGAGCAGCC TGCGTCAGAT TGGCCGGAGG CTGTCTGTCT CTTGATAGGA 
CGCCAGGACC TCTCCAAGCA GGCCTGTGAG GGAAACTTGC CCAAAGGGAA ATCTGTGCTC 
TCCTCAGATG TTCCTTCAGG AACAGAGACT GAAGAGGAGG ATGACGGCAT GAATGACATG 
AATCAGGAGG TCATGTCACT CATATGGAGT GAAGATCTGC GGGTGCAGGA TGTGCGAAGG 
CTGCTTCAAA GTGCGCGTCC GGTACGCGTC AGCGTGGTGC AGTGCCCAGA GCTCAGCGAC 
CACGAGCTCA TCGAGGAGAA GGAGAACAGA TTGCTCCAGT TGTGCCAGCG CACTATGGCC 
CTTCCGGTGG GACGAGGGAT GTTCACTTTG TTTTCGTACC ATCCTGTTCC AACGGAGCCG 
TTGCCTATTC CTAAGCTGAA TTTGACTGGG CGTGCCCCGC CGCGGAATAC AACTGTGGAC 
CTCAGTAGCG GGAACGTGGA CGTACCCCCC AACATGGCCA GCTGGGCCAG CTTCCACAAC 
GGTGTAGCCG CTGGCCTAAA GATCGCCCCG GCCTCTCAGA TTGATTCAGC CTGGATTGTG 
TACAACAAGC CCAAGCACGC TGAGTTGGCC AATGAGTACG CGGGCTTCCT GATGGCCCTG 
GGCCTGAACG GGCACCTCAC CAAGCTGGCC ACTCTCAACA TCCATGACTA CCTGACCAAG 
GGTCACGAAA TGACAAGCAT TGGACTGCTC CTCGGTGTCT CCGCTGCTAA ACTGGGAACC 
ATGGACATGT GTGTCACTCG GCTGCTCAGC ATCCACGTCC CTGCTCTCTT GCCCCCAACG 
TCCACGGAGC TGGATGTCCC TCACAGTGTC CAGGTGGCTG CAGTGGTCGG CATTGGCCTG 
GTGTATCAGG GGACAGCCCA CAGGCACACC GCAGAAGTCC TGTTGGCCGA GATAGGCCGG 
CCTCCTGGTC CCGAGATGGA ATACTGCACA GACAGAGAGT CCTACTCTTT AGCTGCTGGC 
CTGGCCCTGG GCATGGTCTG CTTGGGGCAC GGCAGTAACT TGGTAGGTAT GTCGGACTTG 
AATGTTCCTG AACAGCTTTA TCAGTACATG GTTGGAGGCC ACCGGCGCTT TCAAGCGGGA 
ATGCACAGGG AGAAGCATAA ATCTCCAAGT TACCAAATCA AAGAAGGAGA CACCATAAAT 
GTGGATGTGA CGTGCCCAGG AGCCACGCTT GCTCTGGCTA TGATCTACTT GAAAACCAAC 
AACAGATCCG TCGCAGACTG GCTTCGAGCT CCTGACACTA TGTACCTGCT GGACTTTGTG 
AAGCCAGAGT TCCTGTTGCT TAGGACCCTG GCTCGGTGCC TGATCTTGTG GGATGACATT 
TCACCAGATT CCAAGTGGGT TGATAGCAAT GTCCCTCAGA TTATACGAGA AAATAGTATC 
TCTCTCAGTG AAATCGAATT GCCTTGCTCC GAGGATTTGA ATCTGGAAAC TTTGTCGCAA 
GCACATGTCT ACATAATTGC AGGAGCCTGT TTATCTCTGG GTTTTCGATT TGCTGGCTCA 
GAAAACTTAT CAGCATTTAA CTGTTTGCAT AAATTTGCAA AGGATTTTAT GACTTATTTG 
TCTGCACCTA ATGCTTCTGT GACAGGTCCT TACAACCTGG AGACGTGCCT GAGTGTGGTG 
CTGCTGTCTC TGGCCATGGT CATGGCCGGC TCGGGGAACC TAAAGGTTTT GCAGCTGTGT 
CGCTTCTTGC ACATGAAAAC AGGTGGCGAA ATGAACTATG GTTTTCACTT AGCCCATCAC 
ATGGCCCTTG GACTTCTATT TTTGGGAGGA GGAAGGTACT CGTTGAGCAC CTCCAACTCC 
TCCATCGCTG CCCTCCTGTG TGCCCTTTAC CCACACTTCC CCGCTCACAG CACTGACAAC 
CGATATCATC TCCAGGCCCT ACGGCACCTG TATGTGCTGG CTGCAGAACC ACGGCTGCTG 
GTGCCTGTGG ATGTGGACGC CAACACACCC TGCTATGCCC TCTTGGAGGT CACCTATAAA 
GGCACTCAGT GGTACGAGCA GACCAAAGAA GAGCTGATGG CGCCCACCCT TCTTCCGGAG 
CTCCACCTCC TAAAGCAGAT CAGAGTTAAA GGCCCGCGGT ACTGGGAGCT GCTCATCGAT 
TTAAGCAAGG GGACGCAGCA CTTGAAGTCC ATCCTTTCCA AGGATGGCGT TTTATATGTG 
AAGCTCAGAG CAGGCCAGCT CTCCTACAAG GAGGACCCAA TGGGGTGGCA GAGCTTGCTG 
GCCCAGACTG TCGCTAACCG GAACTCTGAA GCCCGAGCTT TCAAGCCTGA AACAATTTCA 
GCATTCACTT CTGACCCAGC GCTTCTGTCG TTTGCTGAGT ATTTCTGCAA ACCCACTGTG 
AACATGGGCC AGAAGCAGGA AATTCTGGAT CTCTTCTCTT CAGTGCTCTA TGAATGTGTC 
ACCCAGGAGA CCCCGGAGAT GCTACCTGCG TACGTGGCAA TGGATCAGGC CGTGCGGAGA 
CTGGGGCGGG GAGAGGTGTC CGAGACAGCA GAGCTTTGGC AGATCAAGTT GGTGCTGGCC 
TTCTTCAGCT CCAGAAGCCA CCAGGAGCGG CTGCAGAGCC CCCCGAAGCG AGGTTTCTTT 
ATGAGCTCAG AGTTCCTCCC AGTGGTCAAG TGTGCAATTG ACAACACCTT GGACCAGTGG 
CTACAAGCCG GGGGTGACGT GTGTGTGCAT GCCTACCTCA GCGGACAGCC CCGGGACGAG 
TCTCAGCTGA GCGCGCTGGC CTGCTTCCTG GTCTACCACT CGGTGCCGGC CCCGCGGCAC 
CTGCCTCCTG TTGGACTGGA AGGGAGCACA AGCTTCGCTG AACTGCTCTT CAAGTTTAAA 
CAGCTGAAAA TGCCCGTGCG AGCTTTGCTG AGATTGGCTC CTTTGCTCCT TGGAAACCCA 
CAGCCGATGG TTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011376142.1
CDS	269..6106
Translation	MSNFYEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGEEVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEKAYSSDEVEKCICILQSSCINMHSIGGKDYIASLPFQVAGVWPTKYGLLFERSTSAHEAPPGPPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDHAMKIVFLNMDPSIVMTYDTVQSLHSVWVLRRVKTEEENVVLKFSEQGGTPQNVATSSSLTAHLRSLSKGDSPVGSPFQNYSSVHSQSRSTSSPSLHSRSPSISNMAALSRAHSPASGAHSFSGVQRFNLSSHNQSPRRHSISQSPNSHSNGSFLAPETEPIVPELCIEHVWTEAITSMREKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSITNLQAKDAAPVEKLDTMLVLESSGNLVLYTGVVRVGKVFIPGLPAPSLTASRAVPRPSTPLDSISTPEPLSKLLGSLDEAVLLSPVPELRDSSKLHDSLYNEDCTFQQLGTYIHSVRDPVRNRVTLELSNGTMVRITLPEIATSELVQTCLQAIKFILPKEIAVQMLVKWYSVHSAPGSPSYHSEWNLFVICLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSETGSDEDWEYLLNSDYHQSAESHLLSRSLCLSPLEAPQVKGEDSSQSLSLDSSTLLFSHMPAVFFVLHLVYEELKLNTLMGEGSRSLVDLLVQLARDLKLEPYLDHYYRDYPTLVRTTGQVCTVDPGQTGFMHHPSFFTSEPPSIYQWVSSCLKGEGSPPYPYLPGACERSRLVVLSIALYILGDESSVSDESSSQYLSRITPTSQKSQAEQEENRLSVQHSTSVSSLAERLVVWMTSVGFTLRDLETLPFGIALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMNQEVMSLIWSEDLRVQDVRRLLQSARPVRVSVVQCPELSDHELIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLSSGNVDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMCVTRLLSIHVPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLVGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDISPDSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDANTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIRVKGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYVAMDQAVRRLGRGEVSETAELWQIKLVLAFFSSRSHQERLQSPPKRGFFMSSEFLPVVKCAIDNTLDQWLQAGGDVCVHAYLSGQPRDESQLSALACFLVYHSVPAPRHLPPVGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM

Target ORF information:

RefSeq Version	XM_011377840.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011377840.2

ORF Insert Sequence:

ATGTCGAACT TCTATGAAGA AAGGGCAACA ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTCG GTCGAGACCA CTGCAAGCAT CACCCTAATG CTCTGAACCT TCAACTGCGC 
CAGCTGCAGC CGGCTTCTGA GTTATGGTCG TCCGATGGTG CTGCTGGCTT GGTGGGGTCC 
CTTCAGGAGG TTACAATCCA CGAGAAACAG AAGGAAAGCT GGCAGTTAAG GAAAGGCGTA 
AGCGAAATTG GAGAAGAAGT GGATTATGAT GAGGAACTCT ATGTGGCTGG AAATATGGTG 
ATCTGGAGCA AAGGGAGTAA AAGTCAGGCG CTGGCAGTTT ATAAAGCGTT TACAGTTGAC 
AGTCCTGTTC AACAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAA 
GCCTACAGTA GCGATGAAGT AGAGAAATGC ATATGTATAT TGCAAAGTTC GTGTATTAAC 
ATGCACAGCA TAGGAGGAAA GGATTACATA GCTTCTTTAC CTTTTCAGGT TGCCGGTGTC 
TGGCCTACTA AGTATGGACT GTTGTTCGAA CGGAGCACTT CTGCACACGA GGCACCTCCC 
GGCCCGCCCA GAGAACCTTT ACCCACCATG TTCAGCATGC TGCATCCACT GGATGAAATA 
ACCCCACTTG TGTGTAAATC GGGAAGCCTT TTCGGTTCAT CACGGGTGCA ATATGTTGTA 
GATCACGCGA TGAAAATAGT TTTTCTCAAC ATGGACCCCT CCATTGTAAT GACCTACGAC 
ACTGTTCAGA GTCTACATTC TGTGTGGGTT CTCCGGAGAG TTAAAACAGA GGAAGAGAAT 
GTTGTCTTAA AGTTCTCTGA ACAGGGGGGC ACCCCCCAGA ATGTGGCCAC GAGTAGCTCC 
CTCACAGCTC ACCTCAGAAG CCTTTCCAAA GGAGACTCTC CCGTGGGCTC GCCTTTCCAG 
AATTACTCCT CAGTCCACAG TCAGAGCCGC TCCACCTCAT CGCCCAGTCT GCACTCTCGG 
TCTCCTTCCA TCTCCAACAT GGCAGCTCTG AGCCGTGCTC ACTCCCCTGC CTCAGGAGCG 
CACTCTTTCT CAGGGGTGCA AAGGTTCAAC CTTTCAAGCC ACAATCAGTC TCCAAGGAGA 
CACAGCATTT CTCAGTCTCC AAATAGCCAT TCAAATGGCT CTTTCCTTGC GCCAGAAACC 
GAGCCGATTG TTCCTGAACT GTGTATTGAA CATGTATGGA CAGAAGCAAT TACCAGTATG 
AGAGAGAAAA ATTCACAGGC CTCCAAAGTG TTTATTACAT CTGACCTATG TGGGCAGAAG 
TTCTTGTGCT TCTTAGTAGA GTCCCAGCTC CAGTTACGCT GTGTGAAGTT TCAAGAGAGT 
AATGATAAGA CCCAGCTCAT CTTCGGCTCC ATCACCAACC TGCAGGCAAA GGATGCGGCG 
CCAGTGGAGA AGTTAGACAC CATGCTGGTC CTGGAAAGCA GTGGCAACCT GGTGCTATAC 
ACAGGCGTGG TCCGGGTGGG AAAAGTTTTT ATCCCTGGCC TGCCAGCCCC TTCCCTGACA 
GCGTCCCGTG CGGTGCCTCG TCCCAGCACC CCGCTAGACA GCATCAGCAC CCCTGAGCCT 
CTGAGTAAGC TGCTTGGATC CTTGGATGAG GCTGTTCTGT TGTCCCCAGT TCCAGAACTA 
AGAGATTCTT CGAAACTCCA TGATTCTCTC TATAATGAGG ATTGCACCTT CCAGCAGCTT 
GGGACTTATA TCCACTCTGT TAGAGACCCC GTCCGTAATA GAGTGACTCT CGAGCTGAGT 
AACGGCACCA TGGTTAGGAT CACTCTTCCT GAAATTGCCA CCTCTGAATT AGTACAAACA 
TGTTTGCAAG CCATTAAATT TATCCTGCCA AAAGAAATAG CTGTTCAGAT GCTTGTCAAG 
TGGTACAGTG TCCACAGCGC TCCGGGCAGC CCCAGCTATC ACTCAGAGTG GAATTTATTT 
GTGATTTGTC TCATGAACAT GATGGGTTAT AACACAGACC GCTTAGCGTG GACAAGAAAT 
TTTGACTTTG AAGGATCACT TTCTCCAGTC ATCGCACCCA AAAAGGCAAG GCCTTCTGAG 
ACGGGATCTG ATGAGGACTG GGAGTATTTG CTAAATTCAG ACTATCACCA GAGTGCCGAG 
TCTCACCTTC TGAGCAGGTC TCTCTGTCTG AGTCCTTTGG AGGCTCCTCA GGTGAAAGGA 
GAGGACTCAT CACAGAGCCT CAGTCTGGAC TCCTCCACGC TTCTCTTCTC CCACATGCCT 
GCAGTGTTTT TTGTTCTTCA CCTTGTCTAT GAGGAGCTCA AGCTGAACAC TCTGATGGGA 
GAAGGAAGTC GCTCACTTGT TGACCTGCTT GTTCAGTTGG CAAGAGACTT AAAATTGGAG 
CCTTACTTAG ATCATTACTA TAGAGATTAC CCGACGCTTG TCAGAACTAC TGGACAAGTG 
TGCACAGTTG ATCCAGGTCA AACAGGATTT ATGCATCACC CATCATTTTT CACTTCCGAG 
CCACCAAGTA TTTACCAGTG GGTGAGTTCT TGTCTGAAGG GCGAGGGGTC GCCACCCTAC 
CCCTACCTCC CTGGTGCCTG TGAAAGAAGC CGGCTCGTGG TCTTGAGTAT TGCACTCTAC 
ATTCTAGGTG ATGAAAGCTC TGTTTCTGAT GAATCCTCCT CACAGTATTT ATCCAGAATA 
ACTCCAACGT CCCAGAAGTC ACAAGCAGAA CAAGAGGAAA ACAGGCTTAG TGTCCAGCAT 
TCTACATCAG TTTCTAGTCT AGCCGAAAGA TTAGTTGTCT GGATGACTAG TGTAGGATTC 
ACTCTAAGGG ACTTGGAAAC GCTTCCCTTT GGAATTGCTC TTCCCATCAG AGATGCAATT 
TATCACTGTC GGGAGCAGCC TGCGTCAGAT TGGCCGGAGG CTGTCTGTCT CTTGATAGGA 
CGCCAGGACC TCTCCAAGCA GGCCTGTGAG GGAAACTTGC CCAAAGGGAA ATCTGTGCTC 
TCCTCAGATG TTCCTTCAGG AACAGAGACT GAAGAGGAGG ATGACGGCAT GAATGACATG 
AATCAGGAGG TCATGTCACT CATATGGAGT GAAGATCTGC GGGTGCAGGA TGTGCGAAGG 
CTGCTTCAAA GTGCGCGTCC GGTACGCGTC AGCGTGGTGC AGTGCCCAGA GCTCAGCGAC 
CACGAGCTCA TCGAGGAGAA GGAGAACAGA TTGCTCCAGT TGTGCCAGCG CACTATGGCC 
CTTCCGGTGG GACGAGGGAT GTTCACTTTG TTTTCGTACC ATCCTGTTCC AACGGAGCCG 
TTGCCTATTC CTAAGCTGAA TTTGACTGGG CGTGCCCCGC CGCGGAATAC AACTGTGGAC 
CTCAGTAGCG GGAACGTGGA CGTACCCCCC AACATGGCCA GCTGGGCCAG CTTCCACAAC 
GGTGTAGCCG CTGGCCTAAA GATCGCCCCG GCCTCTCAGA TTGATTCAGC CTGGATTGTG 
TACAACAAGC CCAAGCACGC TGAGTTGGCC AATGAGTACG CGGGCTTCCT GATGGCCCTG 
GGCCTGAACG GGCACCTCAC CAAGCTGGCC ACTCTCAACA TCCATGACTA CCTGACCAAG 
GGTCACGAAA TGACAAGCAT TGGACTGCTC CTCGGTGTCT CCGCTGCTAA ACTGGGAACC 
ATGGACATGT GTGTCACTCG GCTGCTCAGC ATCCACGTCC CTGCTCTCTT GCCCCCAACG 
TCCACGGAGC TGGATGTCCC TCACAGTGTC CAGGTGGCTG CAGTGGTCGG CATTGGCCTG 
GTGTATCAGG GGACAGCCCA CAGGCACACC GCAGAAGTCC TGTTGGCCGA GATAGGCCGG 
CCTCCTGGTC CCGAGATGGA ATACTGCACA GACAGAGAGT CCTACTCTTT AGCTGCTGGC 
CTGGCCCTGG GCATGGTCTG CTTGGGGCAC GGCAGTAACT TGGTAGGTAT GTCGGACTTG 
AATGTTCCTG AACAGCTTTA TCAGTACATG GTTGGAGGCC ACCGGCGCTT TCAAGCGGGA 
ATGCACAGGG AGAAGCATAA ATCTCCAAGT TACCAAATCA AAGAAGGAGA CACCATAAAT 
GTGGATGTGA CGTGCCCAGG AGCCACGCTT GCTCTGGCTA TGATCTACTT GAAAACCAAC 
AACAGATCCG TCGCAGACTG GCTTCGAGCT CCTGACACTA TGTACCTGCT GGACTTTGTG 
AAGCCAGAGT TCCTGTTGCT TAGGACCCTG GCTCGGTGCC TGATCTTGTG GGATGACATT 
TCACCAGATT CCAAGTGGGT TGATAGCAAT GTCCCTCAGA TTATACGAGA AAATAGTATC 
TCTCTCAGTG AAATCGAATT GCCTTGCTCC GAGGATTTGA ATCTGGAAAC TTTGTCGCAA 
GCACATGTCT ACATAATTGC AGGAGCCTGT TTATCTCTGG GTTTTCGATT TGCTGGCTCA 
GAAAACTTAT CAGCATTTAA CTGTTTGCAT AAATTTGCAA AGGATTTTAT GACTTATTTG 
TCTGCACCTA ATGCTTCTGT GACAGGTCCT TACAACCTGG AGACGTGCCT GAGTGTGGTG 
CTGCTGTCTC TGGCCATGGT CATGGCCGGC TCGGGGAACC TAAAGGTTTT GCAGCTGTGT 
CGCTTCTTGC ACATGAAAAC AGGTGGCGAA ATGAACTATG GTTTTCACTT AGCCCATCAC 
ATGGCCCTTG GACTTCTATT TTTGGGAGGA GGAAGGTACT CGTTGAGCAC CTCCAACTCC 
TCCATCGCTG CCCTCCTGTG TGCCCTTTAC CCACACTTCC CCGCTCACAG CACTGACAAC 
CGATATCATC TCCAGGCCCT ACGGCACCTG TATGTGCTGG CTGCAGAACC ACGGCTGCTG 
GTGCCTGTGG ATGTGGACGC CAACACACCC TGCTATGCCC TCTTGGAGGT CACCTATAAA 
GGCACTCAGT GGTACGAGCA GACCAAAGAA GAGCTGATGG CGCCCACCCT TCTTCCGGAG 
CTCCACCTCC TAAAGCAGAT CAGAGTTAAA GGCCCGCGGT ACTGGGAGCT GCTCATCGAT 
TTAAGCAAGG GGACGCAGCA CTTGAAGTCC ATCCTTTCCA AGGATGGCGT TTTATATGTG 
AAGCTCAGAG CAGGCCAGCT CTCCTACAAG GAGGACCCAA TGGGGTGGCA GAGCTTGCTG 
GCCCAGACTG TCGCTAACCG GAACTCTGAA GCCCGAGCTT TCAAGCCTGA AACAATTTCA 
GCATTCACTT CTGACCCAGC GCTTCTGTCG TTTGCTGAGT ATTTCTGCAA ACCCACTGTG 
AACATGGGCC AGAAGCAGGA AATTCTGGAT CTCTTCTCTT CAGTGCTCTA TGAATGTGTC 
ACCCAGGAGA CCCCGGAGAT GCTACCTGCG TACGTGGCAA TGGATCAGGC CGTGCGGAGA 
CTGGGGCGGG GAGAGGTGTC CGAGACAGCA GAGCTTTGGC AGATCAAGTT GGTGCTGGCC 
TTCTTCAGCT CCAGAAGCCA CCAGGAGCGG CTGCAGAGCC CCCCGAAGCG AGGTTTCTTT 
ATGAGCTCAG AGTTCCTCCC AGTGGTCAAG TGTGCAATTG ACAACACCTT GGACCAGTGG 
CTACAAGCCG GGGGTGACGT GTGTGTGCAT GCCTACCTCA GCGGACAGCC CCGGGACGAG 
TCTCAGCTGA GCGCGCTGGC CTGCTTCCTG GTCTACCACT CGGTGCCGGC CCCGCGGCAC 
CTGCCTCCTG TTGGACTGGA AGGGAGCACA AGCTTCGCTG AACTGCTCTT CAAGTTTAAA 
CAGCTGAAAA TGCCCGTGCG AGCTTTGCTG AGATTGGCTC CTTTGCTCCT TGGAAACCCA 
CAGCCGATGG TTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_011377840.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011376142.1 Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), mRNA.

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CloneID	OPt59533
Clone ID Related Accession (Same CDS sequence)	XM_011377840.2 , XM_011377840.1 , XM_023521936.1
Accession Version	XM_011377840.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	anaphase-promoting complex subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889104.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACT TCTATGAAGA AAGGGCAACA ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTCG GTCGAGACCA CTGCAAGCAT CACCCTAATG CTCTGAACCT TCAACTGCGC 
CAGCTGCAGC CGGCTTCTGA GTTATGGTCG TCCGATGGTG CTGCTGGCTT GGTGGGGTCC 
CTTCAGGAGG TTACAATCCA CGAGAAACAG AAGGAAAGCT GGCAGTTAAG GAAAGGCGTA 
AGCGAAATTG GAGAAGAAGT GGATTATGAT GAGGAACTCT ATGTGGCTGG AAATATGGTG 
ATCTGGAGCA AAGGGAGTAA AAGTCAGGCG CTGGCAGTTT ATAAAGCGTT TACAGTTGAC 
AGTCCTGTTC AACAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAA 
GCCTACAGTA GCGATGAAGT AGAGAAATGC ATATGTATAT TGCAAAGTTC GTGTATTAAC 
ATGCACAGCA TAGGAGGAAA GGATTACATA GCTTCTTTAC CTTTTCAGGT TGCCGGTGTC 
TGGCCTACTA AGTATGGACT GTTGTTCGAA CGGAGCACTT CTGCACACGA GGCACCTCCC 
GGCCCGCCCA GAGAACCTTT ACCCACCATG TTCAGCATGC TGCATCCACT GGATGAAATA 
ACCCCACTTG TGTGTAAATC GGGAAGCCTT TTCGGTTCAT CACGGGTGCA ATATGTTGTA 
GATCACGCGA TGAAAATAGT TTTTCTCAAC ATGGACCCCT CCATTGTAAT GACCTACGAC 
ACTGTTCAGA GTCTACATTC TGTGTGGGTT CTCCGGAGAG TTAAAACAGA GGAAGAGAAT 
GTTGTCTTAA AGTTCTCTGA ACAGGGGGGC ACCCCCCAGA ATGTGGCCAC GAGTAGCTCC 
CTCACAGCTC ACCTCAGAAG CCTTTCCAAA GGAGACTCTC CCGTGGGCTC GCCTTTCCAG 
AATTACTCCT CAGTCCACAG TCAGAGCCGC TCCACCTCAT CGCCCAGTCT GCACTCTCGG 
TCTCCTTCCA TCTCCAACAT GGCAGCTCTG AGCCGTGCTC ACTCCCCTGC CTCAGGAGCG 
CACTCTTTCT CAGGGGTGCA AAGGTTCAAC CTTTCAAGCC ACAATCAGTC TCCAAGGAGA 
CACAGCATTT CTCAGTCTCC AAATAGCCAT TCAAATGGCT CTTTCCTTGC GCCAGAAACC 
GAGCCGATTG TTCCTGAACT GTGTATTGAA CATGTATGGA CAGAAGCAAT TACCAGTATG 
AGAGAGAAAA ATTCACAGGC CTCCAAAGTG TTTATTACAT CTGACCTATG TGGGCAGAAG 
TTCTTGTGCT TCTTAGTAGA GTCCCAGCTC CAGTTACGCT GTGTGAAGTT TCAAGAGAGT 
AATGATAAGA CCCAGCTCAT CTTCGGCTCC ATCACCAACC TGCAGGCAAA GGATGCGGCG 
CCAGTGGAGA AGTTAGACAC CATGCTGGTC CTGGAAAGCA GTGGCAACCT GGTGCTATAC 
ACAGGCGTGG TCCGGGTGGG AAAAGTTTTT ATCCCTGGCC TGCCAGCCCC TTCCCTGACA 
GCGTCCCGTG CGGTGCCTCG TCCCAGCACC CCGCTAGACA GCATCAGCAC CCCTGAGCCT 
CTGAGTAAGC TGCTTGGATC CTTGGATGAG GCTGTTCTGT TGTCCCCAGT TCCAGAACTA 
AGAGATTCTT CGAAACTCCA TGATTCTCTC TATAATGAGG ATTGCACCTT CCAGCAGCTT 
GGGACTTATA TCCACTCTGT TAGAGACCCC GTCCGTAATA GAGTGACTCT CGAGCTGAGT 
AACGGCACCA TGGTTAGGAT CACTCTTCCT GAAATTGCCA CCTCTGAATT AGTACAAACA 
TGTTTGCAAG CCATTAAATT TATCCTGCCA AAAGAAATAG CTGTTCAGAT GCTTGTCAAG 
TGGTACAGTG TCCACAGCGC TCCGGGCAGC CCCAGCTATC ACTCAGAGTG GAATTTATTT 
GTGATTTGTC TCATGAACAT GATGGGTTAT AACACAGACC GCTTAGCGTG GACAAGAAAT 
TTTGACTTTG AAGGATCACT TTCTCCAGTC ATCGCACCCA AAAAGGCAAG GCCTTCTGAG 
ACGGGATCTG ATGAGGACTG GGAGTATTTG CTAAATTCAG ACTATCACCA GAGTGCCGAG 
TCTCACCTTC TGAGCAGGTC TCTCTGTCTG AGTCCTTTGG AGGCTCCTCA GGTGAAAGGA 
GAGGACTCAT CACAGAGCCT CAGTCTGGAC TCCTCCACGC TTCTCTTCTC CCACATGCCT 
GCAGTGTTTT TTGTTCTTCA CCTTGTCTAT GAGGAGCTCA AGCTGAACAC TCTGATGGGA 
GAAGGAAGTC GCTCACTTGT TGACCTGCTT GTTCAGTTGG CAAGAGACTT AAAATTGGAG 
CCTTACTTAG ATCATTACTA TAGAGATTAC CCGACGCTTG TCAGAACTAC TGGACAAGTG 
TGCACAGTTG ATCCAGGTCA AACAGGATTT ATGCATCACC CATCATTTTT CACTTCCGAG 
CCACCAAGTA TTTACCAGTG GGTGAGTTCT TGTCTGAAGG GCGAGGGGTC GCCACCCTAC 
CCCTACCTCC CTGGTGCCTG TGAAAGAAGC CGGCTCGTGG TCTTGAGTAT TGCACTCTAC 
ATTCTAGGTG ATGAAAGCTC TGTTTCTGAT GAATCCTCCT CACAGTATTT ATCCAGAATA 
ACTCCAACGT CCCAGAAGTC ACAAGCAGAA CAAGAGGAAA ACAGGCTTAG TGTCCAGCAT 
TCTACATCAG TTTCTAGTCT AGCCGAAAGA TTAGTTGTCT GGATGACTAG TGTAGGATTC 
ACTCTAAGGG ACTTGGAAAC GCTTCCCTTT GGAATTGCTC TTCCCATCAG AGATGCAATT 
TATCACTGTC GGGAGCAGCC TGCGTCAGAT TGGCCGGAGG CTGTCTGTCT CTTGATAGGA 
CGCCAGGACC TCTCCAAGCA GGCCTGTGAG GGAAACTTGC CCAAAGGGAA ATCTGTGCTC 
TCCTCAGATG TTCCTTCAGG AACAGAGACT GAAGAGGAGG ATGACGGCAT GAATGACATG 
AATCAGGAGG TCATGTCACT CATATGGAGT GAAGATCTGC GGGTGCAGGA TGTGCGAAGG 
CTGCTTCAAA GTGCGCGTCC GGTACGCGTC AGCGTGGTGC AGTGCCCAGA GCTCAGCGAC 
CACGAGCTCA TCGAGGAGAA GGAGAACAGA TTGCTCCAGT TGTGCCAGCG CACTATGGCC 
CTTCCGGTGG GACGAGGGAT GTTCACTTTG TTTTCGTACC ATCCTGTTCC AACGGAGCCG 
TTGCCTATTC CTAAGCTGAA TTTGACTGGG CGTGCCCCGC CGCGGAATAC AACTGTGGAC 
CTCAGTAGCG GGAACGTGGA CGTACCCCCC AACATGGCCA GCTGGGCCAG CTTCCACAAC 
GGTGTAGCCG CTGGCCTAAA GATCGCCCCG GCCTCTCAGA TTGATTCAGC CTGGATTGTG 
TACAACAAGC CCAAGCACGC TGAGTTGGCC AATGAGTACG CGGGCTTCCT GATGGCCCTG 
GGCCTGAACG GGCACCTCAC CAAGCTGGCC ACTCTCAACA TCCATGACTA CCTGACCAAG 
GGTCACGAAA TGACAAGCAT TGGACTGCTC CTCGGTGTCT CCGCTGCTAA ACTGGGAACC 
ATGGACATGT GTGTCACTCG GCTGCTCAGC ATCCACGTCC CTGCTCTCTT GCCCCCAACG 
TCCACGGAGC TGGATGTCCC TCACAGTGTC CAGGTGGCTG CAGTGGTCGG CATTGGCCTG 
GTGTATCAGG GGACAGCCCA CAGGCACACC GCAGAAGTCC TGTTGGCCGA GATAGGCCGG 
CCTCCTGGTC CCGAGATGGA ATACTGCACA GACAGAGAGT CCTACTCTTT AGCTGCTGGC 
CTGGCCCTGG GCATGGTCTG CTTGGGGCAC GGCAGTAACT TGGTAGGTAT GTCGGACTTG 
AATGTTCCTG AACAGCTTTA TCAGTACATG GTTGGAGGCC ACCGGCGCTT TCAAGCGGGA 
ATGCACAGGG AGAAGCATAA ATCTCCAAGT TACCAAATCA AAGAAGGAGA CACCATAAAT 
GTGGATGTGA CGTGCCCAGG AGCCACGCTT GCTCTGGCTA TGATCTACTT GAAAACCAAC 
AACAGATCCG TCGCAGACTG GCTTCGAGCT CCTGACACTA TGTACCTGCT GGACTTTGTG 
AAGCCAGAGT TCCTGTTGCT TAGGACCCTG GCTCGGTGCC TGATCTTGTG GGATGACATT 
TCACCAGATT CCAAGTGGGT TGATAGCAAT GTCCCTCAGA TTATACGAGA AAATAGTATC 
TCTCTCAGTG AAATCGAATT GCCTTGCTCC GAGGATTTGA ATCTGGAAAC TTTGTCGCAA 
GCACATGTCT ACATAATTGC AGGAGCCTGT TTATCTCTGG GTTTTCGATT TGCTGGCTCA 
GAAAACTTAT CAGCATTTAA CTGTTTGCAT AAATTTGCAA AGGATTTTAT GACTTATTTG 
TCTGCACCTA ATGCTTCTGT GACAGGTCCT TACAACCTGG AGACGTGCCT GAGTGTGGTG 
CTGCTGTCTC TGGCCATGGT CATGGCCGGC TCGGGGAACC TAAAGGTTTT GCAGCTGTGT 
CGCTTCTTGC ACATGAAAAC AGGTGGCGAA ATGAACTATG GTTTTCACTT AGCCCATCAC 
ATGGCCCTTG GACTTCTATT TTTGGGAGGA GGAAGGTACT CGTTGAGCAC CTCCAACTCC 
TCCATCGCTG CCCTCCTGTG TGCCCTTTAC CCACACTTCC CCGCTCACAG CACTGACAAC 
CGATATCATC TCCAGGCCCT ACGGCACCTG TATGTGCTGG CTGCAGAACC ACGGCTGCTG 
GTGCCTGTGG ATGTGGACGC CAACACACCC TGCTATGCCC TCTTGGAGGT CACCTATAAA 
GGCACTCAGT GGTACGAGCA GACCAAAGAA GAGCTGATGG CGCCCACCCT TCTTCCGGAG 
CTCCACCTCC TAAAGCAGAT CAGAGTTAAA GGCCCGCGGT ACTGGGAGCT GCTCATCGAT 
TTAAGCAAGG GGACGCAGCA CTTGAAGTCC ATCCTTTCCA AGGATGGCGT TTTATATGTG 
AAGCTCAGAG CAGGCCAGCT CTCCTACAAG GAGGACCCAA TGGGGTGGCA GAGCTTGCTG 
GCCCAGACTG TCGCTAACCG GAACTCTGAA GCCCGAGCTT TCAAGCCTGA AACAATTTCA 
GCATTCACTT CTGACCCAGC GCTTCTGTCG TTTGCTGAGT ATTTCTGCAA ACCCACTGTG 
AACATGGGCC AGAAGCAGGA AATTCTGGAT CTCTTCTCTT CAGTGCTCTA TGAATGTGTC 
ACCCAGGAGA CCCCGGAGAT GCTACCTGCG TACGTGGCAA TGGATCAGGC CGTGCGGAGA 
CTGGGGCGGG GAGAGGTGTC CGAGACAGCA GAGCTTTGGC AGATCAAGTT GGTGCTGGCC 
TTCTTCAGCT CCAGAAGCCA CCAGGAGCGG CTGCAGAGCC CCCCGAAGCG AGGTTTCTTT 
ATGAGCTCAG AGTTCCTCCC AGTGGTCAAG TGTGCAATTG ACAACACCTT GGACCAGTGG 
CTACAAGCCG GGGGTGACGT GTGTGTGCAT GCCTACCTCA GCGGACAGCC CCGGGACGAG 
TCTCAGCTGA GCGCGCTGGC CTGCTTCCTG GTCTACCACT CGGTGCCGGC CCCGCGGCAC 
CTGCCTCCTG TTGGACTGGA AGGGAGCACA AGCTTCGCTG AACTGCTCTT CAAGTTTAAA 
CAGCTGAAAA TGCCCGTGCG AGCTTTGCTG AGATTGGCTC CTTTGCTCCT TGGAAACCCA 
CAGCCGATGG TTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011376142.1
CDS	258..6095
Translation	MSNFYEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGEEVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEKAYSSDEVEKCICILQSSCINMHSIGGKDYIASLPFQVAGVWPTKYGLLFERSTSAHEAPPGPPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDHAMKIVFLNMDPSIVMTYDTVQSLHSVWVLRRVKTEEENVVLKFSEQGGTPQNVATSSSLTAHLRSLSKGDSPVGSPFQNYSSVHSQSRSTSSPSLHSRSPSISNMAALSRAHSPASGAHSFSGVQRFNLSSHNQSPRRHSISQSPNSHSNGSFLAPETEPIVPELCIEHVWTEAITSMREKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSITNLQAKDAAPVEKLDTMLVLESSGNLVLYTGVVRVGKVFIPGLPAPSLTASRAVPRPSTPLDSISTPEPLSKLLGSLDEAVLLSPVPELRDSSKLHDSLYNEDCTFQQLGTYIHSVRDPVRNRVTLELSNGTMVRITLPEIATSELVQTCLQAIKFILPKEIAVQMLVKWYSVHSAPGSPSYHSEWNLFVICLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSETGSDEDWEYLLNSDYHQSAESHLLSRSLCLSPLEAPQVKGEDSSQSLSLDSSTLLFSHMPAVFFVLHLVYEELKLNTLMGEGSRSLVDLLVQLARDLKLEPYLDHYYRDYPTLVRTTGQVCTVDPGQTGFMHHPSFFTSEPPSIYQWVSSCLKGEGSPPYPYLPGACERSRLVVLSIALYILGDESSVSDESSSQYLSRITPTSQKSQAEQEENRLSVQHSTSVSSLAERLVVWMTSVGFTLRDLETLPFGIALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMNQEVMSLIWSEDLRVQDVRRLLQSARPVRVSVVQCPELSDHELIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLSSGNVDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMCVTRLLSIHVPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLVGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDISPDSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDANTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIRVKGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYVAMDQAVRRLGRGEVSETAELWQIKLVLAFFSSRSHQERLQSPPKRGFFMSSEFLPVVKCAIDNTLDQWLQAGGDVCVHAYLSGQPRDESQLSALACFLVYHSVPAPRHLPPVGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM

Target ORF information:

RefSeq Version	XM_011377840.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011377840.1

ORF Insert Sequence:

ATGTCGAACT TCTATGAAGA AAGGGCAACA ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTCG GTCGAGACCA CTGCAAGCAT CACCCTAATG CTCTGAACCT TCAACTGCGC 
CAGCTGCAGC CGGCTTCTGA GTTATGGTCG TCCGATGGTG CTGCTGGCTT GGTGGGGTCC 
CTTCAGGAGG TTACAATCCA CGAGAAACAG AAGGAAAGCT GGCAGTTAAG GAAAGGCGTA 
AGCGAAATTG GAGAAGAAGT GGATTATGAT GAGGAACTCT ATGTGGCTGG AAATATGGTG 
ATCTGGAGCA AAGGGAGTAA AAGTCAGGCG CTGGCAGTTT ATAAAGCGTT TACAGTTGAC 
AGTCCTGTTC AACAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAA 
GCCTACAGTA GCGATGAAGT AGAGAAATGC ATATGTATAT TGCAAAGTTC GTGTATTAAC 
ATGCACAGCA TAGGAGGAAA GGATTACATA GCTTCTTTAC CTTTTCAGGT TGCCGGTGTC 
TGGCCTACTA AGTATGGACT GTTGTTCGAA CGGAGCACTT CTGCACACGA GGCACCTCCC 
GGCCCGCCCA GAGAACCTTT ACCCACCATG TTCAGCATGC TGCATCCACT GGATGAAATA 
ACCCCACTTG TGTGTAAATC GGGAAGCCTT TTCGGTTCAT CACGGGTGCA ATATGTTGTA 
GATCACGCGA TGAAAATAGT TTTTCTCAAC ATGGACCCCT CCATTGTAAT GACCTACGAC 
ACTGTTCAGA GTCTACATTC TGTGTGGGTT CTCCGGAGAG TTAAAACAGA GGAAGAGAAT 
GTTGTCTTAA AGTTCTCTGA ACAGGGGGGC ACCCCCCAGA ATGTGGCCAC GAGTAGCTCC 
CTCACAGCTC ACCTCAGAAG CCTTTCCAAA GGAGACTCTC CCGTGGGCTC GCCTTTCCAG 
AATTACTCCT CAGTCCACAG TCAGAGCCGC TCCACCTCAT CGCCCAGTCT GCACTCTCGG 
TCTCCTTCCA TCTCCAACAT GGCAGCTCTG AGCCGTGCTC ACTCCCCTGC CTCAGGAGCG 
CACTCTTTCT CAGGGGTGCA AAGGTTCAAC CTTTCAAGCC ACAATCAGTC TCCAAGGAGA 
CACAGCATTT CTCAGTCTCC AAATAGCCAT TCAAATGGCT CTTTCCTTGC GCCAGAAACC 
GAGCCGATTG TTCCTGAACT GTGTATTGAA CATGTATGGA CAGAAGCAAT TACCAGTATG 
AGAGAGAAAA ATTCACAGGC CTCCAAAGTG TTTATTACAT CTGACCTATG TGGGCAGAAG 
TTCTTGTGCT TCTTAGTAGA GTCCCAGCTC CAGTTACGCT GTGTGAAGTT TCAAGAGAGT 
AATGATAAGA CCCAGCTCAT CTTCGGCTCC ATCACCAACC TGCAGGCAAA GGATGCGGCG 
CCAGTGGAGA AGTTAGACAC CATGCTGGTC CTGGAAAGCA GTGGCAACCT GGTGCTATAC 
ACAGGCGTGG TCCGGGTGGG AAAAGTTTTT ATCCCTGGCC TGCCAGCCCC TTCCCTGACA 
GCGTCCCGTG CGGTGCCTCG TCCCAGCACC CCGCTAGACA GCATCAGCAC CCCTGAGCCT 
CTGAGTAAGC TGCTTGGATC CTTGGATGAG GCTGTTCTGT TGTCCCCAGT TCCAGAACTA 
AGAGATTCTT CGAAACTCCA TGATTCTCTC TATAATGAGG ATTGCACCTT CCAGCAGCTT 
GGGACTTATA TCCACTCTGT TAGAGACCCC GTCCGTAATA GAGTGACTCT CGAGCTGAGT 
AACGGCACCA TGGTTAGGAT CACTCTTCCT GAAATTGCCA CCTCTGAATT AGTACAAACA 
TGTTTGCAAG CCATTAAATT TATCCTGCCA AAAGAAATAG CTGTTCAGAT GCTTGTCAAG 
TGGTACAGTG TCCACAGCGC TCCGGGCAGC CCCAGCTATC ACTCAGAGTG GAATTTATTT 
GTGATTTGTC TCATGAACAT GATGGGTTAT AACACAGACC GCTTAGCGTG GACAAGAAAT 
TTTGACTTTG AAGGATCACT TTCTCCAGTC ATCGCACCCA AAAAGGCAAG GCCTTCTGAG 
ACGGGATCTG ATGAGGACTG GGAGTATTTG CTAAATTCAG ACTATCACCA GAGTGCCGAG 
TCTCACCTTC TGAGCAGGTC TCTCTGTCTG AGTCCTTTGG AGGCTCCTCA GGTGAAAGGA 
GAGGACTCAT CACAGAGCCT CAGTCTGGAC TCCTCCACGC TTCTCTTCTC CCACATGCCT 
GCAGTGTTTT TTGTTCTTCA CCTTGTCTAT GAGGAGCTCA AGCTGAACAC TCTGATGGGA 
GAAGGAAGTC GCTCACTTGT TGACCTGCTT GTTCAGTTGG CAAGAGACTT AAAATTGGAG 
CCTTACTTAG ATCATTACTA TAGAGATTAC CCGACGCTTG TCAGAACTAC TGGACAAGTG 
TGCACAGTTG ATCCAGGTCA AACAGGATTT ATGCATCACC CATCATTTTT CACTTCCGAG 
CCACCAAGTA TTTACCAGTG GGTGAGTTCT TGTCTGAAGG GCGAGGGGTC GCCACCCTAC 
CCCTACCTCC CTGGTGCCTG TGAAAGAAGC CGGCTCGTGG TCTTGAGTAT TGCACTCTAC 
ATTCTAGGTG ATGAAAGCTC TGTTTCTGAT GAATCCTCCT CACAGTATTT ATCCAGAATA 
ACTCCAACGT CCCAGAAGTC ACAAGCAGAA CAAGAGGAAA ACAGGCTTAG TGTCCAGCAT 
TCTACATCAG TTTCTAGTCT AGCCGAAAGA TTAGTTGTCT GGATGACTAG TGTAGGATTC 
ACTCTAAGGG ACTTGGAAAC GCTTCCCTTT GGAATTGCTC TTCCCATCAG AGATGCAATT 
TATCACTGTC GGGAGCAGCC TGCGTCAGAT TGGCCGGAGG CTGTCTGTCT CTTGATAGGA 
CGCCAGGACC TCTCCAAGCA GGCCTGTGAG GGAAACTTGC CCAAAGGGAA ATCTGTGCTC 
TCCTCAGATG TTCCTTCAGG AACAGAGACT GAAGAGGAGG ATGACGGCAT GAATGACATG 
AATCAGGAGG TCATGTCACT CATATGGAGT GAAGATCTGC GGGTGCAGGA TGTGCGAAGG 
CTGCTTCAAA GTGCGCGTCC GGTACGCGTC AGCGTGGTGC AGTGCCCAGA GCTCAGCGAC 
CACGAGCTCA TCGAGGAGAA GGAGAACAGA TTGCTCCAGT TGTGCCAGCG CACTATGGCC 
CTTCCGGTGG GACGAGGGAT GTTCACTTTG TTTTCGTACC ATCCTGTTCC AACGGAGCCG 
TTGCCTATTC CTAAGCTGAA TTTGACTGGG CGTGCCCCGC CGCGGAATAC AACTGTGGAC 
CTCAGTAGCG GGAACGTGGA CGTACCCCCC AACATGGCCA GCTGGGCCAG CTTCCACAAC 
GGTGTAGCCG CTGGCCTAAA GATCGCCCCG GCCTCTCAGA TTGATTCAGC CTGGATTGTG 
TACAACAAGC CCAAGCACGC TGAGTTGGCC AATGAGTACG CGGGCTTCCT GATGGCCCTG 
GGCCTGAACG GGCACCTCAC CAAGCTGGCC ACTCTCAACA TCCATGACTA CCTGACCAAG 
GGTCACGAAA TGACAAGCAT TGGACTGCTC CTCGGTGTCT CCGCTGCTAA ACTGGGAACC 
ATGGACATGT GTGTCACTCG GCTGCTCAGC ATCCACGTCC CTGCTCTCTT GCCCCCAACG 
TCCACGGAGC TGGATGTCCC TCACAGTGTC CAGGTGGCTG CAGTGGTCGG CATTGGCCTG 
GTGTATCAGG GGACAGCCCA CAGGCACACC GCAGAAGTCC TGTTGGCCGA GATAGGCCGG 
CCTCCTGGTC CCGAGATGGA ATACTGCACA GACAGAGAGT CCTACTCTTT AGCTGCTGGC 
CTGGCCCTGG GCATGGTCTG CTTGGGGCAC GGCAGTAACT TGGTAGGTAT GTCGGACTTG 
AATGTTCCTG AACAGCTTTA TCAGTACATG GTTGGAGGCC ACCGGCGCTT TCAAGCGGGA 
ATGCACAGGG AGAAGCATAA ATCTCCAAGT TACCAAATCA AAGAAGGAGA CACCATAAAT 
GTGGATGTGA CGTGCCCAGG AGCCACGCTT GCTCTGGCTA TGATCTACTT GAAAACCAAC 
AACAGATCCG TCGCAGACTG GCTTCGAGCT CCTGACACTA TGTACCTGCT GGACTTTGTG 
AAGCCAGAGT TCCTGTTGCT TAGGACCCTG GCTCGGTGCC TGATCTTGTG GGATGACATT 
TCACCAGATT CCAAGTGGGT TGATAGCAAT GTCCCTCAGA TTATACGAGA AAATAGTATC 
TCTCTCAGTG AAATCGAATT GCCTTGCTCC GAGGATTTGA ATCTGGAAAC TTTGTCGCAA 
GCACATGTCT ACATAATTGC AGGAGCCTGT TTATCTCTGG GTTTTCGATT TGCTGGCTCA 
GAAAACTTAT CAGCATTTAA CTGTTTGCAT AAATTTGCAA AGGATTTTAT GACTTATTTG 
TCTGCACCTA ATGCTTCTGT GACAGGTCCT TACAACCTGG AGACGTGCCT GAGTGTGGTG 
CTGCTGTCTC TGGCCATGGT CATGGCCGGC TCGGGGAACC TAAAGGTTTT GCAGCTGTGT 
CGCTTCTTGC ACATGAAAAC AGGTGGCGAA ATGAACTATG GTTTTCACTT AGCCCATCAC 
ATGGCCCTTG GACTTCTATT TTTGGGAGGA GGAAGGTACT CGTTGAGCAC CTCCAACTCC 
TCCATCGCTG CCCTCCTGTG TGCCCTTTAC CCACACTTCC CCGCTCACAG CACTGACAAC 
CGATATCATC TCCAGGCCCT ACGGCACCTG TATGTGCTGG CTGCAGAACC ACGGCTGCTG 
GTGCCTGTGG ATGTGGACGC CAACACACCC TGCTATGCCC TCTTGGAGGT CACCTATAAA 
GGCACTCAGT GGTACGAGCA GACCAAAGAA GAGCTGATGG CGCCCACCCT TCTTCCGGAG 
CTCCACCTCC TAAAGCAGAT CAGAGTTAAA GGCCCGCGGT ACTGGGAGCT GCTCATCGAT 
TTAAGCAAGG GGACGCAGCA CTTGAAGTCC ATCCTTTCCA AGGATGGCGT TTTATATGTG 
AAGCTCAGAG CAGGCCAGCT CTCCTACAAG GAGGACCCAA TGGGGTGGCA GAGCTTGCTG 
GCCCAGACTG TCGCTAACCG GAACTCTGAA GCCCGAGCTT TCAAGCCTGA AACAATTTCA 
GCATTCACTT CTGACCCAGC GCTTCTGTCG TTTGCTGAGT ATTTCTGCAA ACCCACTGTG 
AACATGGGCC AGAAGCAGGA AATTCTGGAT CTCTTCTCTT CAGTGCTCTA TGAATGTGTC 
ACCCAGGAGA CCCCGGAGAT GCTACCTGCG TACGTGGCAA TGGATCAGGC CGTGCGGAGA 
CTGGGGCGGG GAGAGGTGTC CGAGACAGCA GAGCTTTGGC AGATCAAGTT GGTGCTGGCC 
TTCTTCAGCT CCAGAAGCCA CCAGGAGCGG CTGCAGAGCC CCCCGAAGCG AGGTTTCTTT 
ATGAGCTCAG AGTTCCTCCC AGTGGTCAAG TGTGCAATTG ACAACACCTT GGACCAGTGG 
CTACAAGCCG GGGGTGACGT GTGTGTGCAT GCCTACCTCA GCGGACAGCC CCGGGACGAG 
TCTCAGCTGA GCGCGCTGGC CTGCTTCCTG GTCTACCACT CGGTGCCGGC CCCGCGGCAC 
CTGCCTCCTG TTGGACTGGA AGGGAGCACA AGCTTCGCTG AACTGCTCTT CAAGTTTAAA 
CAGCTGAAAA TGCCCGTGCG AGCTTTGCTG AGATTGGCTC CTTTGCTCCT TGGAAACCCA 
CAGCCGATGG TTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_023521936.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023377704.1 Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), transcript variant X2, mRNA.

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CloneID	OPt59533
Clone ID Related Accession (Same CDS sequence)	XM_011377840.2 , XM_011377840.1 , XM_023521936.1
Accession Version	XM_023521936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	anaphase-promoting complex subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889104.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACT TCTATGAAGA AAGGGCAACA ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTCG GTCGAGACCA CTGCAAGCAT CACCCTAATG CTCTGAACCT TCAACTGCGC 
CAGCTGCAGC CGGCTTCTGA GTTATGGTCG TCCGATGGTG CTGCTGGCTT GGTGGGGTCC 
CTTCAGGAGG TTACAATCCA CGAGAAACAG AAGGAAAGCT GGCAGTTAAG GAAAGGCGTA 
AGCGAAATTG GAGAAGAAGT GGATTATGAT GAGGAACTCT ATGTGGCTGG AAATATGGTG 
ATCTGGAGCA AAGGGAGTAA AAGTCAGGCG CTGGCAGTTT ATAAAGCGTT TACAGTTGAC 
AGTCCTGTTC AACAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAA 
GCCTACAGTA GCGATGAAGT AGAGAAATGC ATATGTATAT TGCAAAGTTC GTGTATTAAC 
ATGCACAGCA TAGGAGGAAA GGATTACATA GCTTCTTTAC CTTTTCAGGT TGCCGGTGTC 
TGGCCTACTA AGTATGGACT GTTGTTCGAA CGGAGCACTT CTGCACACGA GGCACCTCCC 
GGCCCGCCCA GAGAACCTTT ACCCACCATG TTCAGCATGC TGCATCCACT GGATGAAATA 
ACCCCACTTG TGTGTAAATC GGGAAGCCTT TTCGGTTCAT CACGGGTGCA ATATGTTGTA 
GATCACGCGA TGAAAATAGT TTTTCTCAAC ATGGACCCCT CCATTGTAAT GACCTACGAC 
ACTGTTCAGA GTCTACATTC TGTGTGGGTT CTCCGGAGAG TTAAAACAGA GGAAGAGAAT 
GTTGTCTTAA AGTTCTCTGA ACAGGGGGGC ACCCCCCAGA ATGTGGCCAC GAGTAGCTCC 
CTCACAGCTC ACCTCAGAAG CCTTTCCAAA GGAGACTCTC CCGTGGGCTC GCCTTTCCAG 
AATTACTCCT CAGTCCACAG TCAGAGCCGC TCCACCTCAT CGCCCAGTCT GCACTCTCGG 
TCTCCTTCCA TCTCCAACAT GGCAGCTCTG AGCCGTGCTC ACTCCCCTGC CTCAGGAGCG 
CACTCTTTCT CAGGGGTGCA AAGGTTCAAC CTTTCAAGCC ACAATCAGTC TCCAAGGAGA 
CACAGCATTT CTCAGTCTCC AAATAGCCAT TCAAATGGCT CTTTCCTTGC GCCAGAAACC 
GAGCCGATTG TTCCTGAACT GTGTATTGAA CATGTATGGA CAGAAGCAAT TACCAGTATG 
AGAGAGAAAA ATTCACAGGC CTCCAAAGTG TTTATTACAT CTGACCTATG TGGGCAGAAG 
TTCTTGTGCT TCTTAGTAGA GTCCCAGCTC CAGTTACGCT GTGTGAAGTT TCAAGAGAGT 
AATGATAAGA CCCAGCTCAT CTTCGGCTCC ATCACCAACC TGCAGGCAAA GGATGCGGCG 
CCAGTGGAGA AGTTAGACAC CATGCTGGTC CTGGAAAGCA GTGGCAACCT GGTGCTATAC 
ACAGGCGTGG TCCGGGTGGG AAAAGTTTTT ATCCCTGGCC TGCCAGCCCC TTCCCTGACA 
GCGTCCCGTG CGGTGCCTCG TCCCAGCACC CCGCTAGACA GCATCAGCAC CCCTGAGCCT 
CTGAGTAAGC TGCTTGGATC CTTGGATGAG GCTGTTCTGT TGTCCCCAGT TCCAGAACTA 
AGAGATTCTT CGAAACTCCA TGATTCTCTC TATAATGAGG ATTGCACCTT CCAGCAGCTT 
GGGACTTATA TCCACTCTGT TAGAGACCCC GTCCGTAATA GAGTGACTCT CGAGCTGAGT 
AACGGCACCA TGGTTAGGAT CACTCTTCCT GAAATTGCCA CCTCTGAATT AGTACAAACA 
TGTTTGCAAG CCATTAAATT TATCCTGCCA AAAGAAATAG CTGTTCAGAT GCTTGTCAAG 
TGGTACAGTG TCCACAGCGC TCCGGGCAGC CCCAGCTATC ACTCAGAGTG GAATTTATTT 
GTGATTTGTC TCATGAACAT GATGGGTTAT AACACAGACC GCTTAGCGTG GACAAGAAAT 
TTTGACTTTG AAGGATCACT TTCTCCAGTC ATCGCACCCA AAAAGGCAAG GCCTTCTGAG 
ACGGGATCTG ATGAGGACTG GGAGTATTTG CTAAATTCAG ACTATCACCA GAGTGCCGAG 
TCTCACCTTC TGAGCAGGTC TCTCTGTCTG AGTCCTTTGG AGGCTCCTCA GGTGAAAGGA 
GAGGACTCAT CACAGAGCCT CAGTCTGGAC TCCTCCACGC TTCTCTTCTC CCACATGCCT 
GCAGTGTTTT TTGTTCTTCA CCTTGTCTAT GAGGAGCTCA AGCTGAACAC TCTGATGGGA 
GAAGGAAGTC GCTCACTTGT TGACCTGCTT GTTCAGTTGG CAAGAGACTT AAAATTGGAG 
CCTTACTTAG ATCATTACTA TAGAGATTAC CCGACGCTTG TCAGAACTAC TGGACAAGTG 
TGCACAGTTG ATCCAGGTCA AACAGGATTT ATGCATCACC CATCATTTTT CACTTCCGAG 
CCACCAAGTA TTTACCAGTG GGTGAGTTCT TGTCTGAAGG GCGAGGGGTC GCCACCCTAC 
CCCTACCTCC CTGGTGCCTG TGAAAGAAGC CGGCTCGTGG TCTTGAGTAT TGCACTCTAC 
ATTCTAGGTG ATGAAAGCTC TGTTTCTGAT GAATCCTCCT CACAGTATTT ATCCAGAATA 
ACTCCAACGT CCCAGAAGTC ACAAGCAGAA CAAGAGGAAA ACAGGCTTAG TGTCCAGCAT 
TCTACATCAG TTTCTAGTCT AGCCGAAAGA TTAGTTGTCT GGATGACTAG TGTAGGATTC 
ACTCTAAGGG ACTTGGAAAC GCTTCCCTTT GGAATTGCTC TTCCCATCAG AGATGCAATT 
TATCACTGTC GGGAGCAGCC TGCGTCAGAT TGGCCGGAGG CTGTCTGTCT CTTGATAGGA 
CGCCAGGACC TCTCCAAGCA GGCCTGTGAG GGAAACTTGC CCAAAGGGAA ATCTGTGCTC 
TCCTCAGATG TTCCTTCAGG AACAGAGACT GAAGAGGAGG ATGACGGCAT GAATGACATG 
AATCAGGAGG TCATGTCACT CATATGGAGT GAAGATCTGC GGGTGCAGGA TGTGCGAAGG 
CTGCTTCAAA GTGCGCGTCC GGTACGCGTC AGCGTGGTGC AGTGCCCAGA GCTCAGCGAC 
CACGAGCTCA TCGAGGAGAA GGAGAACAGA TTGCTCCAGT TGTGCCAGCG CACTATGGCC 
CTTCCGGTGG GACGAGGGAT GTTCACTTTG TTTTCGTACC ATCCTGTTCC AACGGAGCCG 
TTGCCTATTC CTAAGCTGAA TTTGACTGGG CGTGCCCCGC CGCGGAATAC AACTGTGGAC 
CTCAGTAGCG GGAACGTGGA CGTACCCCCC AACATGGCCA GCTGGGCCAG CTTCCACAAC 
GGTGTAGCCG CTGGCCTAAA GATCGCCCCG GCCTCTCAGA TTGATTCAGC CTGGATTGTG 
TACAACAAGC CCAAGCACGC TGAGTTGGCC AATGAGTACG CGGGCTTCCT GATGGCCCTG 
GGCCTGAACG GGCACCTCAC CAAGCTGGCC ACTCTCAACA TCCATGACTA CCTGACCAAG 
GGTCACGAAA TGACAAGCAT TGGACTGCTC CTCGGTGTCT CCGCTGCTAA ACTGGGAACC 
ATGGACATGT GTGTCACTCG GCTGCTCAGC ATCCACGTCC CTGCTCTCTT GCCCCCAACG 
TCCACGGAGC TGGATGTCCC TCACAGTGTC CAGGTGGCTG CAGTGGTCGG CATTGGCCTG 
GTGTATCAGG GGACAGCCCA CAGGCACACC GCAGAAGTCC TGTTGGCCGA GATAGGCCGG 
CCTCCTGGTC CCGAGATGGA ATACTGCACA GACAGAGAGT CCTACTCTTT AGCTGCTGGC 
CTGGCCCTGG GCATGGTCTG CTTGGGGCAC GGCAGTAACT TGGTAGGTAT GTCGGACTTG 
AATGTTCCTG AACAGCTTTA TCAGTACATG GTTGGAGGCC ACCGGCGCTT TCAAGCGGGA 
ATGCACAGGG AGAAGCATAA ATCTCCAAGT TACCAAATCA AAGAAGGAGA CACCATAAAT 
GTGGATGTGA CGTGCCCAGG AGCCACGCTT GCTCTGGCTA TGATCTACTT GAAAACCAAC 
AACAGATCCG TCGCAGACTG GCTTCGAGCT CCTGACACTA TGTACCTGCT GGACTTTGTG 
AAGCCAGAGT TCCTGTTGCT TAGGACCCTG GCTCGGTGCC TGATCTTGTG GGATGACATT 
TCACCAGATT CCAAGTGGGT TGATAGCAAT GTCCCTCAGA TTATACGAGA AAATAGTATC 
TCTCTCAGTG AAATCGAATT GCCTTGCTCC GAGGATTTGA ATCTGGAAAC TTTGTCGCAA 
GCACATGTCT ACATAATTGC AGGAGCCTGT TTATCTCTGG GTTTTCGATT TGCTGGCTCA 
GAAAACTTAT CAGCATTTAA CTGTTTGCAT AAATTTGCAA AGGATTTTAT GACTTATTTG 
TCTGCACCTA ATGCTTCTGT GACAGGTCCT TACAACCTGG AGACGTGCCT GAGTGTGGTG 
CTGCTGTCTC TGGCCATGGT CATGGCCGGC TCGGGGAACC TAAAGGTTTT GCAGCTGTGT 
CGCTTCTTGC ACATGAAAAC AGGTGGCGAA ATGAACTATG GTTTTCACTT AGCCCATCAC 
ATGGCCCTTG GACTTCTATT TTTGGGAGGA GGAAGGTACT CGTTGAGCAC CTCCAACTCC 
TCCATCGCTG CCCTCCTGTG TGCCCTTTAC CCACACTTCC CCGCTCACAG CACTGACAAC 
CGATATCATC TCCAGGCCCT ACGGCACCTG TATGTGCTGG CTGCAGAACC ACGGCTGCTG 
GTGCCTGTGG ATGTGGACGC CAACACACCC TGCTATGCCC TCTTGGAGGT CACCTATAAA 
GGCACTCAGT GGTACGAGCA GACCAAAGAA GAGCTGATGG CGCCCACCCT TCTTCCGGAG 
CTCCACCTCC TAAAGCAGAT CAGAGTTAAA GGCCCGCGGT ACTGGGAGCT GCTCATCGAT 
TTAAGCAAGG GGACGCAGCA CTTGAAGTCC ATCCTTTCCA AGGATGGCGT TTTATATGTG 
AAGCTCAGAG CAGGCCAGCT CTCCTACAAG GAGGACCCAA TGGGGTGGCA GAGCTTGCTG 
GCCCAGACTG TCGCTAACCG GAACTCTGAA GCCCGAGCTT TCAAGCCTGA AACAATTTCA 
GCATTCACTT CTGACCCAGC GCTTCTGTCG TTTGCTGAGT ATTTCTGCAA ACCCACTGTG 
AACATGGGCC AGAAGCAGGA AATTCTGGAT CTCTTCTCTT CAGTGCTCTA TGAATGTGTC 
ACCCAGGAGA CCCCGGAGAT GCTACCTGCG TACGTGGCAA TGGATCAGGC CGTGCGGAGA 
CTGGGGCGGG GAGAGGTGTC CGAGACAGCA GAGCTTTGGC AGATCAAGTT GGTGCTGGCC 
TTCTTCAGCT CCAGAAGCCA CCAGGAGCGG CTGCAGAGCC CCCCGAAGCG AGGTTTCTTT 
ATGAGCTCAG AGTTCCTCCC AGTGGTCAAG TGTGCAATTG ACAACACCTT GGACCAGTGG 
CTACAAGCCG GGGGTGACGT GTGTGTGCAT GCCTACCTCA GCGGACAGCC CCGGGACGAG 
TCTCAGCTGA GCGCGCTGGC CTGCTTCCTG GTCTACCACT CGGTGCCGGC CCCGCGGCAC 
CTGCCTCCTG TTGGACTGGA AGGGAGCACA AGCTTCGCTG AACTGCTCTT CAAGTTTAAA 
CAGCTGAAAA TGCCCGTGCG AGCTTTGCTG AGATTGGCTC CTTTGCTCCT TGGAAACCCA 
CAGCCGATGG TTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023377704.1
CDS	1..5838
Translation	MSNFYEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGEEVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEKAYSSDEVEKCICILQSSCINMHSIGGKDYIASLPFQVAGVWPTKYGLLFERSTSAHEAPPGPPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDHAMKIVFLNMDPSIVMTYDTVQSLHSVWVLRRVKTEEENVVLKFSEQGGTPQNVATSSSLTAHLRSLSKGDSPVGSPFQNYSSVHSQSRSTSSPSLHSRSPSISNMAALSRAHSPASGAHSFSGVQRFNLSSHNQSPRRHSISQSPNSHSNGSFLAPETEPIVPELCIEHVWTEAITSMREKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSITNLQAKDAAPVEKLDTMLVLESSGNLVLYTGVVRVGKVFIPGLPAPSLTASRAVPRPSTPLDSISTPEPLSKLLGSLDEAVLLSPVPELRDSSKLHDSLYNEDCTFQQLGTYIHSVRDPVRNRVTLELSNGTMVRITLPEIATSELVQTCLQAIKFILPKEIAVQMLVKWYSVHSAPGSPSYHSEWNLFVICLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSETGSDEDWEYLLNSDYHQSAESHLLSRSLCLSPLEAPQVKGEDSSQSLSLDSSTLLFSHMPAVFFVLHLVYEELKLNTLMGEGSRSLVDLLVQLARDLKLEPYLDHYYRDYPTLVRTTGQVCTVDPGQTGFMHHPSFFTSEPPSIYQWVSSCLKGEGSPPYPYLPGACERSRLVVLSIALYILGDESSVSDESSSQYLSRITPTSQKSQAEQEENRLSVQHSTSVSSLAERLVVWMTSVGFTLRDLETLPFGIALPIRDAIYHCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMNQEVMSLIWSEDLRVQDVRRLLQSARPVRVSVVQCPELSDHELIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLSSGNVDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMCVTRLLSIHVPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLVGMSDLNVPEQLYQYMVGGHRRFQAGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDISPDSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDANTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIRVKGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYVAMDQAVRRLGRGEVSETAELWQIKLVLAFFSSRSHQERLQSPPKRGFFMSSEFLPVVKCAIDNTLDQWLQAGGDVCVHAYLSGQPRDESQLSALACFLVYHSVPAPRHLPPVGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM

Target ORF information:

RefSeq Version	XM_023521936.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus anaphase promoting complex subunit 1 (ANAPC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023521936.1

ORF Insert Sequence:

ATGTCGAACT TCTATGAAGA AAGGGCAACA ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTCG GTCGAGACCA CTGCAAGCAT CACCCTAATG CTCTGAACCT TCAACTGCGC 
CAGCTGCAGC CGGCTTCTGA GTTATGGTCG TCCGATGGTG CTGCTGGCTT GGTGGGGTCC 
CTTCAGGAGG TTACAATCCA CGAGAAACAG AAGGAAAGCT GGCAGTTAAG GAAAGGCGTA 
AGCGAAATTG GAGAAGAAGT GGATTATGAT GAGGAACTCT ATGTGGCTGG AAATATGGTG 
ATCTGGAGCA AAGGGAGTAA AAGTCAGGCG CTGGCAGTTT ATAAAGCGTT TACAGTTGAC 
AGTCCTGTTC AACAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAA 
GCCTACAGTA GCGATGAAGT AGAGAAATGC ATATGTATAT TGCAAAGTTC GTGTATTAAC 
ATGCACAGCA TAGGAGGAAA GGATTACATA GCTTCTTTAC CTTTTCAGGT TGCCGGTGTC 
TGGCCTACTA AGTATGGACT GTTGTTCGAA CGGAGCACTT CTGCACACGA GGCACCTCCC 
GGCCCGCCCA GAGAACCTTT ACCCACCATG TTCAGCATGC TGCATCCACT GGATGAAATA 
ACCCCACTTG TGTGTAAATC GGGAAGCCTT TTCGGTTCAT CACGGGTGCA ATATGTTGTA 
GATCACGCGA TGAAAATAGT TTTTCTCAAC ATGGACCCCT CCATTGTAAT GACCTACGAC 
ACTGTTCAGA GTCTACATTC TGTGTGGGTT CTCCGGAGAG TTAAAACAGA GGAAGAGAAT 
GTTGTCTTAA AGTTCTCTGA ACAGGGGGGC ACCCCCCAGA ATGTGGCCAC GAGTAGCTCC 
CTCACAGCTC ACCTCAGAAG CCTTTCCAAA GGAGACTCTC CCGTGGGCTC GCCTTTCCAG 
AATTACTCCT CAGTCCACAG TCAGAGCCGC TCCACCTCAT CGCCCAGTCT GCACTCTCGG 
TCTCCTTCCA TCTCCAACAT GGCAGCTCTG AGCCGTGCTC ACTCCCCTGC CTCAGGAGCG 
CACTCTTTCT CAGGGGTGCA AAGGTTCAAC CTTTCAAGCC ACAATCAGTC TCCAAGGAGA 
CACAGCATTT CTCAGTCTCC AAATAGCCAT TCAAATGGCT CTTTCCTTGC GCCAGAAACC 
GAGCCGATTG TTCCTGAACT GTGTATTGAA CATGTATGGA CAGAAGCAAT TACCAGTATG 
AGAGAGAAAA ATTCACAGGC CTCCAAAGTG TTTATTACAT CTGACCTATG TGGGCAGAAG 
TTCTTGTGCT TCTTAGTAGA GTCCCAGCTC CAGTTACGCT GTGTGAAGTT TCAAGAGAGT 
AATGATAAGA CCCAGCTCAT CTTCGGCTCC ATCACCAACC TGCAGGCAAA GGATGCGGCG 
CCAGTGGAGA AGTTAGACAC CATGCTGGTC CTGGAAAGCA GTGGCAACCT GGTGCTATAC 
ACAGGCGTGG TCCGGGTGGG AAAAGTTTTT ATCCCTGGCC TGCCAGCCCC TTCCCTGACA 
GCGTCCCGTG CGGTGCCTCG TCCCAGCACC CCGCTAGACA GCATCAGCAC CCCTGAGCCT 
CTGAGTAAGC TGCTTGGATC CTTGGATGAG GCTGTTCTGT TGTCCCCAGT TCCAGAACTA 
AGAGATTCTT CGAAACTCCA TGATTCTCTC TATAATGAGG ATTGCACCTT CCAGCAGCTT 
GGGACTTATA TCCACTCTGT TAGAGACCCC GTCCGTAATA GAGTGACTCT CGAGCTGAGT 
AACGGCACCA TGGTTAGGAT CACTCTTCCT GAAATTGCCA CCTCTGAATT AGTACAAACA 
TGTTTGCAAG CCATTAAATT TATCCTGCCA AAAGAAATAG CTGTTCAGAT GCTTGTCAAG 
TGGTACAGTG TCCACAGCGC TCCGGGCAGC CCCAGCTATC ACTCAGAGTG GAATTTATTT 
GTGATTTGTC TCATGAACAT GATGGGTTAT AACACAGACC GCTTAGCGTG GACAAGAAAT 
TTTGACTTTG AAGGATCACT TTCTCCAGTC ATCGCACCCA AAAAGGCAAG GCCTTCTGAG 
ACGGGATCTG ATGAGGACTG GGAGTATTTG CTAAATTCAG ACTATCACCA GAGTGCCGAG 
TCTCACCTTC TGAGCAGGTC TCTCTGTCTG AGTCCTTTGG AGGCTCCTCA GGTGAAAGGA 
GAGGACTCAT CACAGAGCCT CAGTCTGGAC TCCTCCACGC TTCTCTTCTC CCACATGCCT 
GCAGTGTTTT TTGTTCTTCA CCTTGTCTAT GAGGAGCTCA AGCTGAACAC TCTGATGGGA 
GAAGGAAGTC GCTCACTTGT TGACCTGCTT GTTCAGTTGG CAAGAGACTT AAAATTGGAG 
CCTTACTTAG ATCATTACTA TAGAGATTAC CCGACGCTTG TCAGAACTAC TGGACAAGTG 
TGCACAGTTG ATCCAGGTCA AACAGGATTT ATGCATCACC CATCATTTTT CACTTCCGAG 
CCACCAAGTA TTTACCAGTG GGTGAGTTCT TGTCTGAAGG GCGAGGGGTC GCCACCCTAC 
CCCTACCTCC CTGGTGCCTG TGAAAGAAGC CGGCTCGTGG TCTTGAGTAT TGCACTCTAC 
ATTCTAGGTG ATGAAAGCTC TGTTTCTGAT GAATCCTCCT CACAGTATTT ATCCAGAATA 
ACTCCAACGT CCCAGAAGTC ACAAGCAGAA CAAGAGGAAA ACAGGCTTAG TGTCCAGCAT 
TCTACATCAG TTTCTAGTCT AGCCGAAAGA TTAGTTGTCT GGATGACTAG TGTAGGATTC 
ACTCTAAGGG ACTTGGAAAC GCTTCCCTTT GGAATTGCTC TTCCCATCAG AGATGCAATT 
TATCACTGTC GGGAGCAGCC TGCGTCAGAT TGGCCGGAGG CTGTCTGTCT CTTGATAGGA 
CGCCAGGACC TCTCCAAGCA GGCCTGTGAG GGAAACTTGC CCAAAGGGAA ATCTGTGCTC 
TCCTCAGATG TTCCTTCAGG AACAGAGACT GAAGAGGAGG ATGACGGCAT GAATGACATG 
AATCAGGAGG TCATGTCACT CATATGGAGT GAAGATCTGC GGGTGCAGGA TGTGCGAAGG 
CTGCTTCAAA GTGCGCGTCC GGTACGCGTC AGCGTGGTGC AGTGCCCAGA GCTCAGCGAC 
CACGAGCTCA TCGAGGAGAA GGAGAACAGA TTGCTCCAGT TGTGCCAGCG CACTATGGCC 
CTTCCGGTGG GACGAGGGAT GTTCACTTTG TTTTCGTACC ATCCTGTTCC AACGGAGCCG 
TTGCCTATTC CTAAGCTGAA TTTGACTGGG CGTGCCCCGC CGCGGAATAC AACTGTGGAC 
CTCAGTAGCG GGAACGTGGA CGTACCCCCC AACATGGCCA GCTGGGCCAG CTTCCACAAC 
GGTGTAGCCG CTGGCCTAAA GATCGCCCCG GCCTCTCAGA TTGATTCAGC CTGGATTGTG 
TACAACAAGC CCAAGCACGC TGAGTTGGCC AATGAGTACG CGGGCTTCCT GATGGCCCTG 
GGCCTGAACG GGCACCTCAC CAAGCTGGCC ACTCTCAACA TCCATGACTA CCTGACCAAG 
GGTCACGAAA TGACAAGCAT TGGACTGCTC CTCGGTGTCT CCGCTGCTAA ACTGGGAACC 
ATGGACATGT GTGTCACTCG GCTGCTCAGC ATCCACGTCC CTGCTCTCTT GCCCCCAACG 
TCCACGGAGC TGGATGTCCC TCACAGTGTC CAGGTGGCTG CAGTGGTCGG CATTGGCCTG 
GTGTATCAGG GGACAGCCCA CAGGCACACC GCAGAAGTCC TGTTGGCCGA GATAGGCCGG 
CCTCCTGGTC CCGAGATGGA ATACTGCACA GACAGAGAGT CCTACTCTTT AGCTGCTGGC 
CTGGCCCTGG GCATGGTCTG CTTGGGGCAC GGCAGTAACT TGGTAGGTAT GTCGGACTTG 
AATGTTCCTG AACAGCTTTA TCAGTACATG GTTGGAGGCC ACCGGCGCTT TCAAGCGGGA 
ATGCACAGGG AGAAGCATAA ATCTCCAAGT TACCAAATCA AAGAAGGAGA CACCATAAAT 
GTGGATGTGA CGTGCCCAGG AGCCACGCTT GCTCTGGCTA TGATCTACTT GAAAACCAAC 
AACAGATCCG TCGCAGACTG GCTTCGAGCT CCTGACACTA TGTACCTGCT GGACTTTGTG 
AAGCCAGAGT TCCTGTTGCT TAGGACCCTG GCTCGGTGCC TGATCTTGTG GGATGACATT 
TCACCAGATT CCAAGTGGGT TGATAGCAAT GTCCCTCAGA TTATACGAGA AAATAGTATC 
TCTCTCAGTG AAATCGAATT GCCTTGCTCC GAGGATTTGA ATCTGGAAAC TTTGTCGCAA 
GCACATGTCT ACATAATTGC AGGAGCCTGT TTATCTCTGG GTTTTCGATT TGCTGGCTCA 
GAAAACTTAT CAGCATTTAA CTGTTTGCAT AAATTTGCAA AGGATTTTAT GACTTATTTG 
TCTGCACCTA ATGCTTCTGT GACAGGTCCT TACAACCTGG AGACGTGCCT GAGTGTGGTG 
CTGCTGTCTC TGGCCATGGT CATGGCCGGC TCGGGGAACC TAAAGGTTTT GCAGCTGTGT 
CGCTTCTTGC ACATGAAAAC AGGTGGCGAA ATGAACTATG GTTTTCACTT AGCCCATCAC 
ATGGCCCTTG GACTTCTATT TTTGGGAGGA GGAAGGTACT CGTTGAGCAC CTCCAACTCC 
TCCATCGCTG CCCTCCTGTG TGCCCTTTAC CCACACTTCC CCGCTCACAG CACTGACAAC 
CGATATCATC TCCAGGCCCT ACGGCACCTG TATGTGCTGG CTGCAGAACC ACGGCTGCTG 
GTGCCTGTGG ATGTGGACGC CAACACACCC TGCTATGCCC TCTTGGAGGT CACCTATAAA 
GGCACTCAGT GGTACGAGCA GACCAAAGAA GAGCTGATGG CGCCCACCCT TCTTCCGGAG 
CTCCACCTCC TAAAGCAGAT CAGAGTTAAA GGCCCGCGGT ACTGGGAGCT GCTCATCGAT 
TTAAGCAAGG GGACGCAGCA CTTGAAGTCC ATCCTTTCCA AGGATGGCGT TTTATATGTG 
AAGCTCAGAG CAGGCCAGCT CTCCTACAAG GAGGACCCAA TGGGGTGGCA GAGCTTGCTG 
GCCCAGACTG TCGCTAACCG GAACTCTGAA GCCCGAGCTT TCAAGCCTGA AACAATTTCA 
GCATTCACTT CTGACCCAGC GCTTCTGTCG TTTGCTGAGT ATTTCTGCAA ACCCACTGTG 
AACATGGGCC AGAAGCAGGA AATTCTGGAT CTCTTCTCTT CAGTGCTCTA TGAATGTGTC 
ACCCAGGAGA CCCCGGAGAT GCTACCTGCG TACGTGGCAA TGGATCAGGC CGTGCGGAGA 
CTGGGGCGGG GAGAGGTGTC CGAGACAGCA GAGCTTTGGC AGATCAAGTT GGTGCTGGCC 
TTCTTCAGCT CCAGAAGCCA CCAGGAGCGG CTGCAGAGCC CCCCGAAGCG AGGTTTCTTT 
ATGAGCTCAG AGTTCCTCCC AGTGGTCAAG TGTGCAATTG ACAACACCTT GGACCAGTGG 
CTACAAGCCG GGGGTGACGT GTGTGTGCAT GCCTACCTCA GCGGACAGCC CCGGGACGAG 
TCTCAGCTGA GCGCGCTGGC CTGCTTCCTG GTCTACCACT CGGTGCCGGC CCCGCGGCAC 
CTGCCTCCTG TTGGACTGGA AGGGAGCACA AGCTTCGCTG AACTGCTCTT CAAGTTTAAA 
CAGCTGAAAA TGCCCGTGCG AGCTTTGCTG AGATTGGCTC CTTTGCTCCT TGGAAACCCA 
CAGCCGATGG TTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ANAPC1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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