HECW2 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2
Entrez Gene ID	105298181
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011369257.1	XP_011367559.1	E3 ubiquitin-protein ligase HECW2
XM_011369257.2	XP_011367559.1	E3 ubiquitin-protein ligase HECW2

Pathways from BioSystems
Gene Ontology

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt53344	XM_011369257.2 Latest version!	Pteropus vampyrus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
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OPt53344	XM_011369257.1	Pteropus vampyrus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2 ( XM_011369257.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011367559.1 Pteropus vampyrus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Price & Availability↑

CloneID	OPt53344
Clone ID Related Accession (Same CDS sequence)	XM_011369257.1 , XM_011369257.2
Accession Version	XM_011369257.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888927.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGAC GTCGAAATCC CCAGATGCGG 
TACACTCTGA GCCCAGAGAA CCTCCAGAGC CTCGCTGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTAC AGCGGGCCAA CAGTGACACG GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTGACTGCTA GCATGTACGA GTACACCCTG GGGCAAGCTC AGAACCTGAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAT AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCTTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACCA CGCCCTGCAT CACCGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG CAGTCAGCAT 
TCTCGGAAGC TCGTTAGCTT TACATTGTCA GTGAATAAAG GGTTGACAAT AGCTGCAGAT 
ATTTCTAGCA ATGTGGACTC TAAATATTCC TTTTTTGCTC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGGCAAGCCA TCGGTGACCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CTGTTGGCAC AATACTCGGA 
GTCAATACTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAAGACAT GCCAGGGGGG 
CATCATGACA GCCCTGTTTG CTCTAATGGA CCAGTGTCTG AGGAGAGTGC CAATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCAACT CTGGAAATAG ACACAGAGGA TTTAACCTCT 
ACTTCTTCAA GGGCCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAGC ACAGTGGCCA TTCCAGGCCT GGCTCAGCTA CCTGCTCGGA GAGGTCCATG 
GGAGCCTCTC CAAAACTGAG GAGTAGTTTT CCCACCGACA CAAGACTCAA TGCCATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGTTA TCCCTCTTCT 
TTGGAGGAGG AAGGAGGGTT AATAATGTTT AGCAGGACAT CAAGAACTGA TGATGTGAGC 
CTGACGTCTC AGACAAAGCC AGAAGACAGC CCAATGGAGA TTGAGGAAGC CTCCACAAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCGGAA AATCTTCCAG ATCTTGCAGA GAACTCTGTA 
CCCTTGGGCT CAGTTCCAGA TGAAAGTGAA AATGGCCCGG AGTCTCAACC CCGTGCTGAC 
CAGGACAGCA CCGAATTGTG TGGCTCTCAA GAGGTAGATC AGCCCACGAG TGGGGCAGAC 
ACAGGGACTT CTGACACATC TGGAGGAAGC CGAAGAGCCA TCAGTGAGAC TGAGTCCCTT 
GATCAGGGGT CTGAGCCTTC ACAGGTGTCC TCTGAAACAG AACCCAGTGA CCCCGCCAGG 
ACAGAGAGTG TGAGTGAGGC GAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGCGCT 
GACAGCTCCT GCAATGAGAG CGTTACCACG CAGCTGTCCT CAGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCGCGGTT TCCCGAAACC CCAGCCTTTT CTTCCCAGGA GGAGGAAGAC 
GGAGCCTGTG CAGCAGAGCC CACGAGCAGC GGCCCCGCAG AAGGGTCACA GGATTCCATA 
TGCACTCCTG GTTCTTTACC TGTGGTGCAA GTGCCCAGCG GAGAGGAGGA AGGGCCAGGG 
GCAGAAATGG CTGCTTTACC TGACCAGGAC GAGGTGGGGG AGGTCTGGCA GCGGAGGGGC 
AGCCTAGAGG GAGCTGCTGC TGCTACTGAG AGCCAGCCCC AAGAAGAGGG TGATGCTGGG 
GATGCCCAGG GGGCTTGTGA AGGGGCCACT GCCCAAGAAG AGGGCGCTAC TGGAGGTTCT 
CAAGCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATCGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCCGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AAATGGAGCA GCTGAACCGG 
AGATATCAGA GTATCCGCAG AACCATGACT AATGAGAGGC CTGAAGAAAA TACCAATGCT 
ATTGATGGGG CAGGGGAGGA AGCTGACTTT CACCAAGCCA GTGCAGATTT CCGCCGGGAG 
AATGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTCTTACA GTCTCCCCCT 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACTGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTTGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAGC TGGAGCTGCC GAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATTG ATCCCCGGCT CCCACTGCAA 
AGCAGTCGAC CCACCAGCGC GCTGGTCCAT CGGCAGCACC TGACGAGGCA GCGCAGCCAC 
AGTGCGGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCGAGTACAT TCAATACAGT CAGTAGGCCA CAATACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCGTT TCTGCGCCAA CCCAATATCT TCGAAATTCT ACAGGAACGC 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT ACGAACTGAA 
GGGACCCCAG GATTGGTGCG CCTTTCAAGT GATGCAGACC TTGTTATGTT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC TTATGTGCCT CCACATGCCT TACTCCACCC CAGCTACTGT 
CAGTCTCCAC GTGGCTCCCC AGTGTCCTCT CCCCAGAACT CGCCAGGTAC GCAGCGTGCC 
AATGCCCGGG CTCCTGCCCC TTACAAGCGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAATTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TACTTGAAGA TGCTTTTAAT CAGATTATGG GCTACTCCCG AAAAGACCTG 
CAGAGAAATA AGCTATATGT CACATTCGTC GGGGAGGAAG GGTTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT CGTGTCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC TTTTGTGGAC 
AATCACCACG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCGCT AATACACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCC TTTTATAAGG CTCTTCTCAG AATTCTGTGT 
GACCTTAGTG ATCTGGAATA CCTTGATGAA GAGTTCCACC AGAGCTTGCA GTGGATGAAA 
GACAATGATA TCCATGACAT CCTGGACCTC ACATTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAATA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG GATGGTGAAA TGGAGGATCG AGAGGGGTGT TGTGCAACAG 
ACAGAGAGCT TAGTGCGCGG CTTCTACGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT TGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATATAG AGGAGGATAT CATGACAATC ATATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAGCGACTAA GGCTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGGAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACCGCCCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCCCC CTACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011367559.1
CDS	1..4596
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSVNKGLTIAADISSNVDSKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESANGTPKHSFRTSSTLEIDTEDLTSTSSRASPPRGRQDSLNDYLDAIEHSGHSRPGSATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRTSRTDDVSLTSQTKPEDSPMEIEEASTNEAASFEDKPENLPDLAENSVPLGSVPDESENGPESQPRADQDSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAEMAALPDQDEVGEVWQRRGSLEGAAAATESQPQEEGDAGDAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011369257.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011369257.1

ORF Insert Sequence:

ATGGCCAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGAC GTCGAAATCC CCAGATGCGG 
TACACTCTGA GCCCAGAGAA CCTCCAGAGC CTCGCTGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTAC AGCGGGCCAA CAGTGACACG GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTGACTGCTA GCATGTACGA GTACACCCTG GGGCAAGCTC AGAACCTGAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAT AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCTTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACCA CGCCCTGCAT CACCGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG CAGTCAGCAT 
TCTCGGAAGC TCGTTAGCTT TACATTGTCA GTGAATAAAG GGTTGACAAT AGCTGCAGAT 
ATTTCTAGCA ATGTGGACTC TAAATATTCC TTTTTTGCTC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGGCAAGCCA TCGGTGACCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CTGTTGGCAC AATACTCGGA 
GTCAATACTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAAGACAT GCCAGGGGGG 
CATCATGACA GCCCTGTTTG CTCTAATGGA CCAGTGTCTG AGGAGAGTGC CAATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCAACT CTGGAAATAG ACACAGAGGA TTTAACCTCT 
ACTTCTTCAA GGGCCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAGC ACAGTGGCCA TTCCAGGCCT GGCTCAGCTA CCTGCTCGGA GAGGTCCATG 
GGAGCCTCTC CAAAACTGAG GAGTAGTTTT CCCACCGACA CAAGACTCAA TGCCATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGTTA TCCCTCTTCT 
TTGGAGGAGG AAGGAGGGTT AATAATGTTT AGCAGGACAT CAAGAACTGA TGATGTGAGC 
CTGACGTCTC AGACAAAGCC AGAAGACAGC CCAATGGAGA TTGAGGAAGC CTCCACAAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCGGAA AATCTTCCAG ATCTTGCAGA GAACTCTGTA 
CCCTTGGGCT CAGTTCCAGA TGAAAGTGAA AATGGCCCGG AGTCTCAACC CCGTGCTGAC 
CAGGACAGCA CCGAATTGTG TGGCTCTCAA GAGGTAGATC AGCCCACGAG TGGGGCAGAC 
ACAGGGACTT CTGACACATC TGGAGGAAGC CGAAGAGCCA TCAGTGAGAC TGAGTCCCTT 
GATCAGGGGT CTGAGCCTTC ACAGGTGTCC TCTGAAACAG AACCCAGTGA CCCCGCCAGG 
ACAGAGAGTG TGAGTGAGGC GAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGCGCT 
GACAGCTCCT GCAATGAGAG CGTTACCACG CAGCTGTCCT CAGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCGCGGTT TCCCGAAACC CCAGCCTTTT CTTCCCAGGA GGAGGAAGAC 
GGAGCCTGTG CAGCAGAGCC CACGAGCAGC GGCCCCGCAG AAGGGTCACA GGATTCCATA 
TGCACTCCTG GTTCTTTACC TGTGGTGCAA GTGCCCAGCG GAGAGGAGGA AGGGCCAGGG 
GCAGAAATGG CTGCTTTACC TGACCAGGAC GAGGTGGGGG AGGTCTGGCA GCGGAGGGGC 
AGCCTAGAGG GAGCTGCTGC TGCTACTGAG AGCCAGCCCC AAGAAGAGGG TGATGCTGGG 
GATGCCCAGG GGGCTTGTGA AGGGGCCACT GCCCAAGAAG AGGGCGCTAC TGGAGGTTCT 
CAAGCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATCGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCCGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AAATGGAGCA GCTGAACCGG 
AGATATCAGA GTATCCGCAG AACCATGACT AATGAGAGGC CTGAAGAAAA TACCAATGCT 
ATTGATGGGG CAGGGGAGGA AGCTGACTTT CACCAAGCCA GTGCAGATTT CCGCCGGGAG 
AATGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTCTTACA GTCTCCCCCT 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACTGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTTGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAGC TGGAGCTGCC GAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATTG ATCCCCGGCT CCCACTGCAA 
AGCAGTCGAC CCACCAGCGC GCTGGTCCAT CGGCAGCACC TGACGAGGCA GCGCAGCCAC 
AGTGCGGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCGAGTACAT TCAATACAGT CAGTAGGCCA CAATACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCGTT TCTGCGCCAA CCCAATATCT TCGAAATTCT ACAGGAACGC 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT ACGAACTGAA 
GGGACCCCAG GATTGGTGCG CCTTTCAAGT GATGCAGACC TTGTTATGTT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC TTATGTGCCT CCACATGCCT TACTCCACCC CAGCTACTGT 
CAGTCTCCAC GTGGCTCCCC AGTGTCCTCT CCCCAGAACT CGCCAGGTAC GCAGCGTGCC 
AATGCCCGGG CTCCTGCCCC TTACAAGCGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAATTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TACTTGAAGA TGCTTTTAAT CAGATTATGG GCTACTCCCG AAAAGACCTG 
CAGAGAAATA AGCTATATGT CACATTCGTC GGGGAGGAAG GGTTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT CGTGTCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC TTTTGTGGAC 
AATCACCACG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCGCT AATACACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCC TTTTATAAGG CTCTTCTCAG AATTCTGTGT 
GACCTTAGTG ATCTGGAATA CCTTGATGAA GAGTTCCACC AGAGCTTGCA GTGGATGAAA 
GACAATGATA TCCATGACAT CCTGGACCTC ACATTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAATA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG GATGGTGAAA TGGAGGATCG AGAGGGGTGT TGTGCAACAG 
ACAGAGAGCT TAGTGCGCGG CTTCTACGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT TGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATATAG AGGAGGATAT CATGACAATC ATATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAGCGACTAA GGCTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGGAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACCGCCCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCCCC CTACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_011369257.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011367559.1 Pteropus vampyrus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Price & Availability↑

CloneID	OPt53344
Clone ID Related Accession (Same CDS sequence)	XM_011369257.1 , XM_011369257.2
Accession Version	XM_011369257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888927.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011369257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGAC GTCGAAATCC CCAGATGCGG 
TACACTCTGA GCCCAGAGAA CCTCCAGAGC CTCGCTGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTAC AGCGGGCCAA CAGTGACACG GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTGACTGCTA GCATGTACGA GTACACCCTG GGGCAAGCTC AGAACCTGAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAT AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCTTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACCA CGCCCTGCAT CACCGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG CAGTCAGCAT 
TCTCGGAAGC TCGTTAGCTT TACATTGTCA GTGAATAAAG GGTTGACAAT AGCTGCAGAT 
ATTTCTAGCA ATGTGGACTC TAAATATTCC TTTTTTGCTC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGGCAAGCCA TCGGTGACCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CTGTTGGCAC AATACTCGGA 
GTCAATACTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAAGACAT GCCAGGGGGG 
CATCATGACA GCCCTGTTTG CTCTAATGGA CCAGTGTCTG AGGAGAGTGC CAATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCAACT CTGGAAATAG ACACAGAGGA TTTAACCTCT 
ACTTCTTCAA GGGCCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAGC ACAGTGGCCA TTCCAGGCCT GGCTCAGCTA CCTGCTCGGA GAGGTCCATG 
GGAGCCTCTC CAAAACTGAG GAGTAGTTTT CCCACCGACA CAAGACTCAA TGCCATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGTTA TCCCTCTTCT 
TTGGAGGAGG AAGGAGGGTT AATAATGTTT AGCAGGACAT CAAGAACTGA TGATGTGAGC 
CTGACGTCTC AGACAAAGCC AGAAGACAGC CCAATGGAGA TTGAGGAAGC CTCCACAAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCGGAA AATCTTCCAG ATCTTGCAGA GAACTCTGTA 
CCCTTGGGCT CAGTTCCAGA TGAAAGTGAA AATGGCCCGG AGTCTCAACC CCGTGCTGAC 
CAGGACAGCA CCGAATTGTG TGGCTCTCAA GAGGTAGATC AGCCCACGAG TGGGGCAGAC 
ACAGGGACTT CTGACACATC TGGAGGAAGC CGAAGAGCCA TCAGTGAGAC TGAGTCCCTT 
GATCAGGGGT CTGAGCCTTC ACAGGTGTCC TCTGAAACAG AACCCAGTGA CCCCGCCAGG 
ACAGAGAGTG TGAGTGAGGC GAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGCGCT 
GACAGCTCCT GCAATGAGAG CGTTACCACG CAGCTGTCCT CAGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCGCGGTT TCCCGAAACC CCAGCCTTTT CTTCCCAGGA GGAGGAAGAC 
GGAGCCTGTG CAGCAGAGCC CACGAGCAGC GGCCCCGCAG AAGGGTCACA GGATTCCATA 
TGCACTCCTG GTTCTTTACC TGTGGTGCAA GTGCCCAGCG GAGAGGAGGA AGGGCCAGGG 
GCAGAAATGG CTGCTTTACC TGACCAGGAC GAGGTGGGGG AGGTCTGGCA GCGGAGGGGC 
AGCCTAGAGG GAGCTGCTGC TGCTACTGAG AGCCAGCCCC AAGAAGAGGG TGATGCTGGG 
GATGCCCAGG GGGCTTGTGA AGGGGCCACT GCCCAAGAAG AGGGCGCTAC TGGAGGTTCT 
CAAGCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATCGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCCGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AAATGGAGCA GCTGAACCGG 
AGATATCAGA GTATCCGCAG AACCATGACT AATGAGAGGC CTGAAGAAAA TACCAATGCT 
ATTGATGGGG CAGGGGAGGA AGCTGACTTT CACCAAGCCA GTGCAGATTT CCGCCGGGAG 
AATGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTCTTACA GTCTCCCCCT 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACTGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTTGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAGC TGGAGCTGCC GAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATTG ATCCCCGGCT CCCACTGCAA 
AGCAGTCGAC CCACCAGCGC GCTGGTCCAT CGGCAGCACC TGACGAGGCA GCGCAGCCAC 
AGTGCGGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCGAGTACAT TCAATACAGT CAGTAGGCCA CAATACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCGTT TCTGCGCCAA CCCAATATCT TCGAAATTCT ACAGGAACGC 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT ACGAACTGAA 
GGGACCCCAG GATTGGTGCG CCTTTCAAGT GATGCAGACC TTGTTATGTT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC TTATGTGCCT CCACATGCCT TACTCCACCC CAGCTACTGT 
CAGTCTCCAC GTGGCTCCCC AGTGTCCTCT CCCCAGAACT CGCCAGGTAC GCAGCGTGCC 
AATGCCCGGG CTCCTGCCCC TTACAAGCGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAATTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TACTTGAAGA TGCTTTTAAT CAGATTATGG GCTACTCCCG AAAAGACCTG 
CAGAGAAATA AGCTATATGT CACATTCGTC GGGGAGGAAG GGTTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT CGTGTCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC TTTTGTGGAC 
AATCACCACG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCGCT AATACACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCC TTTTATAAGG CTCTTCTCAG AATTCTGTGT 
GACCTTAGTG ATCTGGAATA CCTTGATGAA GAGTTCCACC AGAGCTTGCA GTGGATGAAA 
GACAATGATA TCCATGACAT CCTGGACCTC ACATTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAATA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG GATGGTGAAA TGGAGGATCG AGAGGGGTGT TGTGCAACAG 
ACAGAGAGCT TAGTGCGCGG CTTCTACGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT TGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATATAG AGGAGGATAT CATGACAATC ATATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAGCGACTAA GGCTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGGAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACCGCCCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCCCC CTACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011367559.1
CDS	30..4625
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSQHSRKLVSFTLSVNKGLTIAADISSNVDSKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNTVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESANGTPKHSFRTSSTLEIDTEDLTSTSSRASPPRGRQDSLNDYLDAIEHSGHSRPGSATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRTSRTDDVSLTSQTKPEDSPMEIEEASTNEAASFEDKPENLPDLAENSVPLGSVPDESENGPESQPRADQDSTELCGSQEVDQPTSGADTGTSDTSGGSRRAISETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTPGSLPVVQVPSGEEEGPGAEMAALPDQDEVGEVWQRRGSLEGAAAATESQPQEEGDAGDAQGACEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011369257.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011369257.2

ORF Insert Sequence:

ATGGCCAGCT CAGCCCGGGA GCACCTGCTT TTTGTGCGAC GTCGAAATCC CCAGATGCGG 
TACACTCTGA GCCCAGAGAA CCTCCAGAGC CTCGCTGCCC AGAGCTCCAT GCCAGAGAAC 
ATGACCCTAC AGCGGGCCAA CAGTGACACG GACCTGGTGA CTTCTGAGAG CCGCTCCAGC 
TTGACTGCTA GCATGTACGA GTACACCCTG GGGCAAGCTC AGAACCTGAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAT AGGGGTGTGA CTGGAACACA AAAAGGGCAA 
ATTGTATGGA GAATTGAGCC TGGGCCTTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT 
AAATACTACC ACGGCATTAG TGGAGCCCTG CGAGCCACCA CGCCCTGCAT CACCGTGAAG 
AACCCTGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCAGG CAGTCAGCAT 
TCTCGGAAGC TCGTTAGCTT TACATTGTCA GTGAATAAAG GGTTGACAAT AGCTGCAGAT 
ATTTCTAGCA ATGTGGACTC TAAATATTCC TTTTTTGCTC TTCTTACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAGCGTTT TCTGGGGAAA 
CTAACCATTC CAGTCCAGAG GCTGCTGGAG CGGCAAGCCA TCGGTGACCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAAAGTG 
GAGGTTACAT CTTCTGTGCA TGAAGATGCT TCTCCAGAAG CTGTTGGCAC AATACTCGGA 
GTCAATACTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAAGACAT GCCAGGGGGG 
CATCATGACA GCCCTGTTTG CTCTAATGGA CCAGTGTCTG AGGAGAGTGC CAATGGGACC 
CCCAAGCATT CTTTCAGGAC TAGCTCAACT CTGGAAATAG ACACAGAGGA TTTAACCTCT 
ACTTCTTCAA GGGCCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAGC ACAGTGGCCA TTCCAGGCCT GGCTCAGCTA CCTGCTCGGA GAGGTCCATG 
GGAGCCTCTC CAAAACTGAG GAGTAGTTTT CCCACCGACA CAAGACTCAA TGCCATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGTTA TCCCTCTTCT 
TTGGAGGAGG AAGGAGGGTT AATAATGTTT AGCAGGACAT CAAGAACTGA TGATGTGAGC 
CTGACGTCTC AGACAAAGCC AGAAGACAGC CCAATGGAGA TTGAGGAAGC CTCCACAAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCGGAA AATCTTCCAG ATCTTGCAGA GAACTCTGTA 
CCCTTGGGCT CAGTTCCAGA TGAAAGTGAA AATGGCCCGG AGTCTCAACC CCGTGCTGAC 
CAGGACAGCA CCGAATTGTG TGGCTCTCAA GAGGTAGATC AGCCCACGAG TGGGGCAGAC 
ACAGGGACTT CTGACACATC TGGAGGAAGC CGAAGAGCCA TCAGTGAGAC TGAGTCCCTT 
GATCAGGGGT CTGAGCCTTC ACAGGTGTCC TCTGAAACAG AACCCAGTGA CCCCGCCAGG 
ACAGAGAGTG TGAGTGAGGC GAGCACCAGG CCTGAGGGAG AGAGTGACCT GGAAGGCGCT 
GACAGCTCCT GCAATGAGAG CGTTACCACG CAGCTGTCCT CAGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GTGCGCGGTT TCCCGAAACC CCAGCCTTTT CTTCCCAGGA GGAGGAAGAC 
GGAGCCTGTG CAGCAGAGCC CACGAGCAGC GGCCCCGCAG AAGGGTCACA GGATTCCATA 
TGCACTCCTG GTTCTTTACC TGTGGTGCAA GTGCCCAGCG GAGAGGAGGA AGGGCCAGGG 
GCAGAAATGG CTGCTTTACC TGACCAGGAC GAGGTGGGGG AGGTCTGGCA GCGGAGGGGC 
AGCCTAGAGG GAGCTGCTGC TGCTACTGAG AGCCAGCCCC AAGAAGAGGG TGATGCTGGG 
GATGCCCAGG GGGCTTGTGA AGGGGCCACT GCCCAAGAAG AGGGCGCTAC TGGAGGTTCT 
CAAGCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TGCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATCGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA TGTAAACAGA ACCACAACGT GGCAGCGGCC CACAGCTCCC 
CCCGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AAATGGAGCA GCTGAACCGG 
AGATATCAGA GTATCCGCAG AACCATGACT AATGAGAGGC CTGAAGAAAA TACCAATGCT 
ATTGATGGGG CAGGGGAGGA AGCTGACTTT CACCAAGCCA GTGCAGATTT CCGCCGGGAG 
AATGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTCTTACA GTCTCCCCCT 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACTGTGCTGC ATTCTAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC GTGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTTGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAGC TGGAGCTGCC GAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATTG ATCCCCGGCT CCCACTGCAA 
AGCAGTCGAC CCACCAGCGC GCTGGTCCAT CGGCAGCACC TGACGAGGCA GCGCAGCCAC 
AGTGCGGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCA 
TCGAGTACAT TCAATACAGT CAGTAGGCCA CAATACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCGTT TCTGCGCCAA CCCAATATCT TCGAAATTCT ACAGGAACGC 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT ACGAACTGAA 
GGGACCCCAG GATTGGTGCG CCTTTCAAGT GATGCAGACC TTGTTATGTT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC TTATGTGCCT CCACATGCCT TACTCCACCC CAGCTACTGT 
CAGTCTCCAC GTGGCTCCCC AGTGTCCTCT CCCCAGAACT CGCCAGGTAC GCAGCGTGCC 
AATGCCCGGG CTCCTGCCCC TTACAAGCGA GATTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAATTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TACTTGAAGA TGCTTTTAAT CAGATTATGG GCTACTCCCG AAAAGACCTG 
CAGAGAAATA AGCTATATGT CACATTCGTC GGGGAGGAAG GGTTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT CGTGTCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC TTTTGTGGAC 
AATCACCACG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCGCT AATACACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCC TTTTATAAGG CTCTTCTCAG AATTCTGTGT 
GACCTTAGTG ATCTGGAATA CCTTGATGAA GAGTTCCACC AGAGCTTGCA GTGGATGAAA 
GACAATGATA TCCATGACAT CCTGGACCTC ACATTCACTG TGAATGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAATA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG GATGGTGAAA TGGAGGATCG AGAGGGGTGT TGTGCAACAG 
ACAGAGAGCT TAGTGCGCGG CTTCTACGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT TGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATATAG AGGAGGATAT CATGACAATC ATATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAGCGACTAA GGCTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGGAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACCGCCCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCCCC CTACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECW2 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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