COL5A3 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A3
Entrez Gene ID	101115457
Full Name	collagen type V alpha 3 chain
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004009289.3	XP_004009338.2	collagen alpha-3(V) chain isoform X1
XM_012135955.2	XP_011991345.1	collagen alpha-3(V) chain isoform X2
XM_012135956.2	XP_011991346.1	collagen alpha-3(V) chain isoform X3
XM_012135957.2	XP_011991347.1	collagen alpha-3(V) chain isoform X4
XM_027969601.1	XP_027825402.1	collagen alpha-3(V) chain isoform X1
XM_027969602.1	XP_027825403.1	collagen alpha-3(V) chain isoform X2

Pathways from BioSystems
Gene Ontology

The following COL5A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv17333	XM_012135955.2 Latest version!	Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv17334	XM_012135956.2 Latest version!	Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv17335	XM_012135957.2 Latest version!	Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm49458	XM_004009289.3 Latest version!	Ovis aries collagen, type V, alpha 3 (COL5A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm80267	XM_027969601.1 Latest version!	Ovis aries collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm80268	XM_027969602.1 Latest version!	Ovis aries collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A3 ( XM_012135955.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011991345.1 Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X2, mRNA.

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CloneID	OOv17333
Clone ID Related Accession (Same CDS sequence)	XM_012135955.2
Accession Version	XM_012135955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	collagen alpha-3(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942413.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012135955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGCA GATCCTGTGG ATGTGCTGAA GGCGCTGGGC 
ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGCCCCGGCC TCTGCCCCCA GAGGGCCCCA 
GAGGGTGACC GAGCATTCCG GGTGGGCAAG ACCAGCACAC TTGGTGTCCC CACGCAAGAG 
CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG 
CCAACCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GTGGCGTCCG GCAGCTGGGC 
TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG 
GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG 
ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCGTGTTGG CTCAGGGACC TCGATTCATC 
AGTACAGCGG GACTCACTGT GCTAGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA 
GATGTCCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCCG CCTTCCAGGC ATGTGAGCGG 
TACCTTCCTG GCTGTGATGA CCTGGATCCT ACAGTCACCA GGGCCCCTCA GGGGGAGCCA 
GAAACCCCTC CCGCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAGAAAGG GCGAGGTCGT 
AAGGGGAAGG GCAGGAAGAA GAAGCTCAAG GAAGCTTTGG CTGCGAGTCC GCCTCCTGGG 
CCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCACCTCTG 
TCTCCGACAT CCACGCCTTT GGTCATCACC ACAGCTGTGA CCTTTGGTAG CAATGGCACC 
ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAATTGAAC TGGAGACCCT GGAGACTGAG 
TTAGCCAGAG AAGATGAAGA AGAAGATGGT CCCACCATGG GCCCCCTCTT CCGGGCAGCA 
GAACAGTCAT GGAATACTGA ATTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA 
GGAGAACCAG CCGTGGTTGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCAGGA 
CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGGCCCC CAGGATTCCC TGGAGACCGT 
GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TTGATGGGAT CCGGGGACTC 
CCAGGCACTG TGATCATGAT ACCGTTCCAG TTTGCAGGAG GCTCTCACAA AGGACCCCCA 
GTATCCTTCC TGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT CTCTATGAAA 
GGCCCTCCTG GCCCGGTGGG GCTCACTGGA CGCCCAGGTC CCGTGGGCCT CCCTGGGTAT 
CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCTCAGG GTCCCCGAGG GCTTCAGGGA 
CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCC CTGGAGCAGA TGGGGCTCGG 
GGCCTCCCAG GCACCACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG 
CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA 
GAAGATGGGC GGAAGGGAGT AGAGGGACCT CCGGGGCCCA CTGGCCAGGC GGGGGAGCCG 
GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACA CCCGGGTGTG 
ACTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC 
CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CTGGCCCCCA GGGGCCCATC 
GGCACTCCTG GGGAGAAGGG TCCCCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT 
GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT 
CCAACAGGGT CAGCAGGCCC TCCGGGCTAT CCGGGACCTC GGGGTGTGAA GGGTACTTCT 
GGCAACCGAG GCCTCCAGGG GGAGAAAGGA GAGAGGGGAG AGGATGGTTT CCCAGGCTTC 
AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCTG GACCTCCAGG TCCTCGGGGA 
GAGGATGGTC CTGAAGGGCT CAAGGGGCAG GAGGGGCTGG TTGGCGAGGA GGGTCCCCCA 
GGCTCGGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TTCCAGGTTA TCCAGGACGT 
CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCCCTGG GGCCATTAGG AGAAAAAGGG 
AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT 
GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CTAAGGGTGA TGTGGGCCAG 
GATGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG 
TTCTCCGGAC CAAAGGGTCC CCCTGGTCCC CAGGGGAAAG ATGGGCTACC TGGACACCCT 
GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGAGTC 
GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCTCCAGGG 
CCCCCTGGAC CTCCAGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG 
GGGGACGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC 
CCTGGTCCTC ATGGAGCCCC CGGGGAACCA GGACCAACTG GTTTAAAGGG TGACAAGGGC 
CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA 
GGGGGCATTG GGCTTCCTGG TCAAAGTGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG 
AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCCACTG GTAAAGATGG CATCCCCGGG 
CCCCTGGGCC TTCCTGGACC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG 
GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGAG ACAAGGGAGA TGCGGGCCCG 
CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCAG GCTCTCCAGG AGCAGATGGG 
GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGG 
GGCTTCCCTG GCACGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA 
AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCATG GAGCTCCAGG CCCTCGGGGC 
CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCC 
GGCGCTGTGG GTGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCAGGGACC 
CCAGGCGCCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG 
GCTGCTGGAC CCCCAGGCAA GAAAGGCCCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG 
GGCCCCGCAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA 
GGTGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC 
GCTTCTGGGG AGCCTGGTGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG 
GGTCGAGAAG GCAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC CGATGGACCC 
CCAGGGAGGA CAGGCCCAGT GGGGGCTGTA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT 
CTTCGTGGGA TCCCTGGACC TGTGGGTGAA CCAGGCCTCC TGGGACCTCC TGGACTGCTA 
GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC 
AAGGGAGAAA AGGGCCACAT TGGATTAACT GGTCTCATCG GCCCCCCTGG GGAGGCTGGT 
GAGAAAGGGG ATCAGGGGTT GCCAGGAGTC CAAGGCCCCC CTGGCCTCCA GGGAGAACCG 
GGTTCCCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT 
CCAGGACAGA AAGGCTCAAA GGGGTCCCCA GGAACCCCAG GTCCTCGTGG AGACACCGGC 
CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC 
CGACGTTCCC TGCCAGGCTT GGGGACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
ATGGCGTCGC TGAACTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC 
CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCAC ATCTGCCGGA TGGGGAATAC 
TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCACTCA GGGTTTTCTG TAACTTCACG 
GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACAGTGAA ACTGGCCTCT 
TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC 
TACGTGGATG CCGATGGGGC TCCGGTGAGT GTGGTACAGC TGACCTTCCT GAAGCTGCTG 
AGTGCCACGG CCCGCCAGAG CTTCACGTTC ACCTGCCAGA ACTCGGCCGC GTGGCTGGAC 
GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGCTG 
TCCTTCAACC AGACGGCAGC CGCCACCATC ACGGTCCCCT ACGATGGCTG CAGGCTCCGG 
AAGGGACAGT CGAAGACCCT CTTTGAGTTC AACTCCTCCC GAGTGGGATT TCTGCCCTTG 
TTGGATGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC 
CCTGTCTGCT TTAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011991345.1
CDS	172..5412
Translation	MGSRRGPGQPRAGLCLLLASLQLLPRTQAADPVDVLKALGMRGGQAGIPEGPGLCPQRAPEGDRAFRVGKTSTLGVPTQELFQDGHFPENFSILITLRGQPTNRSVLLSIYDEGGVRQLGLALGPALDLLGDSFSPLPQQVNLTDGRWHRVAVSVDGGMVTLVADCEPQLPVLAQGPRFISTAGLTVLGTQDLGEETFEGDVQELVISPDPQAAFQACERYLPGCDDLDPTVTRAPQGEPETPPARRKGKGKGKKKGRGRKGKGRKKKLKEALAASPPPGPLDNQTSTDIPKTETPEPPLSPTSTPLVITTAVTFGSNGTILEEGLDPDNGIELETLETELAREDEEEDGPTMGPLFRAAEQSWNTEFQIFPGAGEKGEKGEPAVVEQGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGIRGLPGTVIMIPFQFAGGSHKGPPVSFLQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGEMGEMGPQGPRGLQGPHGPPGREGKMGRPGADGARGLPGTTGPKGDRGFDGLPGLPGEKGQRGDFGYVGKPGPPGEDGRKGVEGPPGPTGQAGEPGPRGLIGPRGSPGPLGHPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNPGSQGIPGPQGPIGTPGEKGPPGNPGIPGLPGSEGPPGHPGHEGPTGEKGAQGPTGSAGPPGYPGPRGVKGTSGNRGLQGEKGERGEDGFPGFKGDAGLKGDRGQPGPPGPRGEDGPEGLKGQEGLVGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGLEGERGPPGARGERGQPGATGQPGPKGDVGQDGAPGFPGEKGLPGLQGPPGFSGPKGPPGPQGKDGLPGHPGQRGELGFQGQTGPPGPAGVVGPQGKTGEAGPLGERGPPGPPGPPGEQGLPGLEGREGAKGDVGPLGPLGKEGPPGPRGFPGPHGAPGEPGPTGLKGDKGPPGPVGANGSPGERGPVGPAGGIGLPGQSGGQGPVGPTGEKGSPGERGAPGPTGKDGIPGPLGLPGPPGAAGPSGEEGDKGEVGAPGHKGSKGDKGDAGPPGPTGIRGLVGHPGSPGADGAQGRRGPPGLFGQKGDDGVRGFPGTIGPPGLQGMPGPAGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGPPGLPGGVGQPGAVGEKGEPGEPGDPGPPGTPGAPGPRGEVGEKGDTGPSGAAGPPGKKGPPGEDGAKGNVGPAGLPGDLGPPGDPGVSGQGGPPGEKGNPGDVGGPGPPGASGEPGAPGPPGKRGPSGRMGREGREGEKGAKGEPGPDGPPGRTGPVGAVGPPGRVGPEGLRGIPGPVGEPGLLGPPGLLGPPGPLGPSGLPGLKGDAGPKGEKGHIGLTGLIGPPGEAGEKGDQGLPGVQGPPGLQGEPGSPGPIGSLGHPGPQGVVGPPGQKGSKGSPGTPGPRGDTGPPGPPGPPGPSAELHGLRRRRRSLPGLGTPEGGLEEVLASMASLNFELEQLRRPPGTAERPGLVCKELHRSHPHLPDGEYWIDPNQGCARDALRVFCNFTAGGETCLYPDKKFETVKLASWSKEKPGGWYSTFRRGKKFSYVDADGAPVSVVQLTFLKLLSATARQSFTFTCQNSAAWLDEAAGDHSRSARFLGANGEELSFNQTAAATITVPYDGCRLRKGQSKTLFEFNSSRVGFLPLLDVAAADFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_012135955.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012135955.2

ORF Insert Sequence:

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGCA GATCCTGTGG ATGTGCTGAA GGCGCTGGGC 
ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGCCCCGGCC TCTGCCCCCA GAGGGCCCCA 
GAGGGTGACC GAGCATTCCG GGTGGGCAAG ACCAGCACAC TTGGTGTCCC CACGCAAGAG 
CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG 
CCAACCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GTGGCGTCCG GCAGCTGGGC 
TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG 
GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG 
ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCGTGTTGG CTCAGGGACC TCGATTCATC 
AGTACAGCGG GACTCACTGT GCTAGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA 
GATGTCCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCCG CCTTCCAGGC ATGTGAGCGG 
TACCTTCCTG GCTGTGATGA CCTGGATCCT ACAGTCACCA GGGCCCCTCA GGGGGAGCCA 
GAAACCCCTC CCGCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAGAAAGG GCGAGGTCGT 
AAGGGGAAGG GCAGGAAGAA GAAGCTCAAG GAAGCTTTGG CTGCGAGTCC GCCTCCTGGG 
CCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCACCTCTG 
TCTCCGACAT CCACGCCTTT GGTCATCACC ACAGCTGTGA CCTTTGGTAG CAATGGCACC 
ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAATTGAAC TGGAGACCCT GGAGACTGAG 
TTAGCCAGAG AAGATGAAGA AGAAGATGGT CCCACCATGG GCCCCCTCTT CCGGGCAGCA 
GAACAGTCAT GGAATACTGA ATTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA 
GGAGAACCAG CCGTGGTTGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCAGGA 
CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGGCCCC CAGGATTCCC TGGAGACCGT 
GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TTGATGGGAT CCGGGGACTC 
CCAGGCACTG TGATCATGAT ACCGTTCCAG TTTGCAGGAG GCTCTCACAA AGGACCCCCA 
GTATCCTTCC TGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT CTCTATGAAA 
GGCCCTCCTG GCCCGGTGGG GCTCACTGGA CGCCCAGGTC CCGTGGGCCT CCCTGGGTAT 
CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCTCAGG GTCCCCGAGG GCTTCAGGGA 
CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCC CTGGAGCAGA TGGGGCTCGG 
GGCCTCCCAG GCACCACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG 
CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA 
GAAGATGGGC GGAAGGGAGT AGAGGGACCT CCGGGGCCCA CTGGCCAGGC GGGGGAGCCG 
GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACA CCCGGGTGTG 
ACTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC 
CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CTGGCCCCCA GGGGCCCATC 
GGCACTCCTG GGGAGAAGGG TCCCCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT 
GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT 
CCAACAGGGT CAGCAGGCCC TCCGGGCTAT CCGGGACCTC GGGGTGTGAA GGGTACTTCT 
GGCAACCGAG GCCTCCAGGG GGAGAAAGGA GAGAGGGGAG AGGATGGTTT CCCAGGCTTC 
AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCTG GACCTCCAGG TCCTCGGGGA 
GAGGATGGTC CTGAAGGGCT CAAGGGGCAG GAGGGGCTGG TTGGCGAGGA GGGTCCCCCA 
GGCTCGGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TTCCAGGTTA TCCAGGACGT 
CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCCCTGG GGCCATTAGG AGAAAAAGGG 
AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT 
GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CTAAGGGTGA TGTGGGCCAG 
GATGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG 
TTCTCCGGAC CAAAGGGTCC CCCTGGTCCC CAGGGGAAAG ATGGGCTACC TGGACACCCT 
GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGAGTC 
GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCTCCAGGG 
CCCCCTGGAC CTCCAGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG 
GGGGACGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC 
CCTGGTCCTC ATGGAGCCCC CGGGGAACCA GGACCAACTG GTTTAAAGGG TGACAAGGGC 
CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA 
GGGGGCATTG GGCTTCCTGG TCAAAGTGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG 
AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCCACTG GTAAAGATGG CATCCCCGGG 
CCCCTGGGCC TTCCTGGACC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG 
GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGAG ACAAGGGAGA TGCGGGCCCG 
CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCAG GCTCTCCAGG AGCAGATGGG 
GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGG 
GGCTTCCCTG GCACGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA 
AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCATG GAGCTCCAGG CCCTCGGGGC 
CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCC 
GGCGCTGTGG GTGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCAGGGACC 
CCAGGCGCCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG 
GCTGCTGGAC CCCCAGGCAA GAAAGGCCCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG 
GGCCCCGCAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA 
GGTGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC 
GCTTCTGGGG AGCCTGGTGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG 
GGTCGAGAAG GCAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC CGATGGACCC 
CCAGGGAGGA CAGGCCCAGT GGGGGCTGTA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT 
CTTCGTGGGA TCCCTGGACC TGTGGGTGAA CCAGGCCTCC TGGGACCTCC TGGACTGCTA 
GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC 
AAGGGAGAAA AGGGCCACAT TGGATTAACT GGTCTCATCG GCCCCCCTGG GGAGGCTGGT 
GAGAAAGGGG ATCAGGGGTT GCCAGGAGTC CAAGGCCCCC CTGGCCTCCA GGGAGAACCG 
GGTTCCCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT 
CCAGGACAGA AAGGCTCAAA GGGGTCCCCA GGAACCCCAG GTCCTCGTGG AGACACCGGC 
CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC 
CGACGTTCCC TGCCAGGCTT GGGGACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
ATGGCGTCGC TGAACTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC 
CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCAC ATCTGCCGGA TGGGGAATAC 
TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCACTCA GGGTTTTCTG TAACTTCACG 
GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACAGTGAA ACTGGCCTCT 
TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC 
TACGTGGATG CCGATGGGGC TCCGGTGAGT GTGGTACAGC TGACCTTCCT GAAGCTGCTG 
AGTGCCACGG CCCGCCAGAG CTTCACGTTC ACCTGCCAGA ACTCGGCCGC GTGGCTGGAC 
GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGCTG 
TCCTTCAACC AGACGGCAGC CGCCACCATC ACGGTCCCCT ACGATGGCTG CAGGCTCCGG 
AAGGGACAGT CGAAGACCCT CTTTGAGTTC AACTCCTCCC GAGTGGGATT TCTGCCCTTG 
TTGGATGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC 
CCTGTCTGCT TTAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_012135956.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011991346.1 Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X3, mRNA.

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CloneID	OOv17334
Clone ID Related Accession (Same CDS sequence)	XM_012135956.2
Accession Version	XM_012135956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	collagen alpha-3(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942413.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012135956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC GCTGGGCATG 
CGGGGGGGCC AGGCTGGGAT CCCTGAGGGC CCCGGCCTCT GCCCCCAGAG GGCCCCAGAG 
GGTGACCGAG CATTCCGGGT GGGCAAGACC AGCACACTTG GTGTCCCCAC GCAAGAGCTC 
TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCA 
ACCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGTG GCGTCCGGCA GCTGGGCTTG 
GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC 
AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC 
CTGGTGGCTG ATTGTGAACC TCAGCTCCCC GTGTTGGCTC AGGGACCTCG ATTCATCAGT 
ACAGCGGGAC TCACTGTGCT AGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT 
GTCCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCCGCCT TCCAGGCATG TGAGCGGTAC 
CTTCCTGGCT GTGATGACCT GGATCCTACA GTCACCAGGG CCCCTCAGGG GGAGCCAGAA 
ACCCCTCCCG CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AGAAAGGGCG AGGTCGTAAG 
GGGAAGGGCA GGAAGAAGAA GCTCAAGGAA GCTTTGGCTG CGAGTCCGCC TCCTGGGCCC 
CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC ACCTCTGTCT 
CCGACATCCA CGCCTTTGGT CATCACCACA GCTGTGACCT TTGGTAGCAA TGGCACCATC 
CTAGAGGAGG GCCTGGACCC TGACAATGGA ATTGAACTGG AGACCCTGGA GACTGAGTTA 
GCCAGAGAAG ATGAAGAAGA AGATGGTCCC ACCATGGGCC CCCTCTTCCG GGCAGCAGAA 
CAGTCATGGA ATACTGAATT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA 
GAACCAGCCG TGGTTGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCAGGACCC 
CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGGCCCCCAG GATTCCCTGG AGACCGTGGT 
CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATTG ATGGGATCCG GGGACTCCCA 
GGCACTGTGA TCATGATACC GTTCCAGTTT GCAGGAGGCT CTCACAAAGG ACCCCCAGTA 
TCCTTCCTGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTCTC TATGAAAGGC 
CCTCCTGGCC CGGTGGGGCT CACTGGACGC CCAGGTCCCG TGGGCCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCTCAGGGTC CCCGAGGGCT TCAGGGACCT 
CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCCCTG GAGCAGATGG GGCTCGGGGC 
CTCCCAGGCA CCACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT 
GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAA 
GATGGGCGGA AGGGAGTAGA GGGACCTCCG GGGCCCACTG GCCAGGCGGG GGAGCCGGGC 
CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACACCC GGGTGTGACT 
GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC 
CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCTG GCCCCCAGGG GCCCATCGGC 
ACTCCTGGGG AGAAGGGTCC CCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA 
GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA 
ACAGGGTCAG CAGGCCCTCC GGGCTATCCG GGACCTCGGG GTGTGAAGGG TACTTCTGGC 
AACCGAGGCC TCCAGGGGGA GAAAGGAGAG AGGGGAGAGG ATGGTTTCCC AGGCTTCAAG 
GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCTGGAC CTCCAGGTCC TCGGGGAGAG 
GATGGTCCTG AAGGGCTCAA GGGGCAGGAG GGGCTGGTTG GCGAGGAGGG TCCCCCAGGC 
TCGGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTTC CAGGTTATCC AGGACGTCCA 
GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCCCTGGGGC CATTAGGAGA AAAAGGGAAG 
CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA 
GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCTA AGGGTGATGT GGGCCAGGAT 
GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC 
TCCGGACCAA AGGGTCCCCC TGGTCCCCAG GGGAAAGATG GGCTACCTGG ACACCCTGGA 
CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGAGTCGTG 
GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC TCCAGGGCCC 
CCTGGACCTC CAGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GACGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT 
GGTCCTCATG GAGCCCCCGG GGAACCAGGA CCAACTGGTT TAAAGGGTGA CAAGGGCCCC 
CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG 
GGCATTGGGC TTCCTGGTCA AAGTGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG 
GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCCACTGGTA AAGATGGCAT CCCCGGGCCC 
CTGGGCCTTC CTGGACCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA 
GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGAGACA AGGGAGATGC GGGCCCGCCT 
GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCAGGCT CTCCAGGAGC AGATGGGGCT 
CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGGGGC 
TTCCCTGGCA CGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG 
GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCATGGAG CTCCAGGCCC TCGGGGCCCC 
CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCCGGC 
GCTGTGGGTG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC AGGGACCCCA 
GGCGCCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT 
GCTGGACCCC CAGGCAAGAA AGGCCCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC 
CCCGCAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGGT 
GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT 
TCTGGGGAGC CTGGTGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT 
CGAGAAGGCA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCCGA TGGACCCCCA 
GGGAGGACAG GCCCAGTGGG GGCTGTAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT 
CGTGGGATCC CTGGACCTGT GGGTGAACCA GGCCTCCTGG GACCTCCTGG ACTGCTAGGC 
CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG 
GGAGAAAAGG GCCACATTGG ATTAACTGGT CTCATCGGCC CCCCTGGGGA GGCTGGTGAG 
AAAGGGGATC AGGGGTTGCC AGGAGTCCAA GGCCCCCCTG GCCTCCAGGG AGAACCGGGT 
TCCCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA 
GGACAGAAAG GCTCAAAGGG GTCCCCAGGA ACCCCAGGTC CTCGTGGAGA CACCGGCCCC 
CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA 
CGTTCCCTGC CAGGCTTGGG GACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG 
GCGTCGCTGA ACTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG 
GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCACATC TGCCGGATGG GGAATACTGG 
ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCACTCAGGG TTTTCTGTAA CTTCACGGCA 
GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CAGTGAAACT GGCCTCTTGG 
TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC 
GTGGATGCCG ATGGGGCTCC GGTGAGTGTG GTACAGCTGA CCTTCCTGAA GCTGCTGAGT 
GCCACGGCCC GCCAGAGCTT CACGTTCACC TGCCAGAACT CGGCCGCGTG GCTGGACGAA 
GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGCTGTCC 
TTCAACCAGA CGGCAGCCGC CACCATCACG GTCCCCTACG ATGGCTGCAG GCTCCGGAAG 
GGACAGTCGA AGACCCTCTT TGAGTTCAAC TCCTCCCGAG TGGGATTTCT GCCCTTGTTG 
GATGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT 
GTCTGCTTTA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011991346.1
CDS	172..5409
Translation	MGSRRGPGQPRAGLCLLLASLQLLPRTQADPVDVLKALGMRGGQAGIPEGPGLCPQRAPEGDRAFRVGKTSTLGVPTQELFQDGHFPENFSILITLRGQPTNRSVLLSIYDEGGVRQLGLALGPALDLLGDSFSPLPQQVNLTDGRWHRVAVSVDGGMVTLVADCEPQLPVLAQGPRFISTAGLTVLGTQDLGEETFEGDVQELVISPDPQAAFQACERYLPGCDDLDPTVTRAPQGEPETPPARRKGKGKGKKKGRGRKGKGRKKKLKEALAASPPPGPLDNQTSTDIPKTETPEPPLSPTSTPLVITTAVTFGSNGTILEEGLDPDNGIELETLETELAREDEEEDGPTMGPLFRAAEQSWNTEFQIFPGAGEKGEKGEPAVVEQGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGIRGLPGTVIMIPFQFAGGSHKGPPVSFLQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGEMGEMGPQGPRGLQGPHGPPGREGKMGRPGADGARGLPGTTGPKGDRGFDGLPGLPGEKGQRGDFGYVGKPGPPGEDGRKGVEGPPGPTGQAGEPGPRGLIGPRGSPGPLGHPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNPGSQGIPGPQGPIGTPGEKGPPGNPGIPGLPGSEGPPGHPGHEGPTGEKGAQGPTGSAGPPGYPGPRGVKGTSGNRGLQGEKGERGEDGFPGFKGDAGLKGDRGQPGPPGPRGEDGPEGLKGQEGLVGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGLEGERGPPGARGERGQPGATGQPGPKGDVGQDGAPGFPGEKGLPGLQGPPGFSGPKGPPGPQGKDGLPGHPGQRGELGFQGQTGPPGPAGVVGPQGKTGEAGPLGERGPPGPPGPPGEQGLPGLEGREGAKGDVGPLGPLGKEGPPGPRGFPGPHGAPGEPGPTGLKGDKGPPGPVGANGSPGERGPVGPAGGIGLPGQSGGQGPVGPTGEKGSPGERGAPGPTGKDGIPGPLGLPGPPGAAGPSGEEGDKGEVGAPGHKGSKGDKGDAGPPGPTGIRGLVGHPGSPGADGAQGRRGPPGLFGQKGDDGVRGFPGTIGPPGLQGMPGPAGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGPPGLPGGVGQPGAVGEKGEPGEPGDPGPPGTPGAPGPRGEVGEKGDTGPSGAAGPPGKKGPPGEDGAKGNVGPAGLPGDLGPPGDPGVSGQGGPPGEKGNPGDVGGPGPPGASGEPGAPGPPGKRGPSGRMGREGREGEKGAKGEPGPDGPPGRTGPVGAVGPPGRVGPEGLRGIPGPVGEPGLLGPPGLLGPPGPLGPSGLPGLKGDAGPKGEKGHIGLTGLIGPPGEAGEKGDQGLPGVQGPPGLQGEPGSPGPIGSLGHPGPQGVVGPPGQKGSKGSPGTPGPRGDTGPPGPPGPPGPSAELHGLRRRRRSLPGLGTPEGGLEEVLASMASLNFELEQLRRPPGTAERPGLVCKELHRSHPHLPDGEYWIDPNQGCARDALRVFCNFTAGGETCLYPDKKFETVKLASWSKEKPGGWYSTFRRGKKFSYVDADGAPVSVVQLTFLKLLSATARQSFTFTCQNSAAWLDEAAGDHSRSARFLGANGEELSFNQTAAATITVPYDGCRLRKGQSKTLFEFNSSRVGFLPLLDVAAADFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_012135956.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012135956.2

ORF Insert Sequence:

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC GCTGGGCATG 
CGGGGGGGCC AGGCTGGGAT CCCTGAGGGC CCCGGCCTCT GCCCCCAGAG GGCCCCAGAG 
GGTGACCGAG CATTCCGGGT GGGCAAGACC AGCACACTTG GTGTCCCCAC GCAAGAGCTC 
TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCA 
ACCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGTG GCGTCCGGCA GCTGGGCTTG 
GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC 
AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC 
CTGGTGGCTG ATTGTGAACC TCAGCTCCCC GTGTTGGCTC AGGGACCTCG ATTCATCAGT 
ACAGCGGGAC TCACTGTGCT AGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT 
GTCCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCCGCCT TCCAGGCATG TGAGCGGTAC 
CTTCCTGGCT GTGATGACCT GGATCCTACA GTCACCAGGG CCCCTCAGGG GGAGCCAGAA 
ACCCCTCCCG CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AGAAAGGGCG AGGTCGTAAG 
GGGAAGGGCA GGAAGAAGAA GCTCAAGGAA GCTTTGGCTG CGAGTCCGCC TCCTGGGCCC 
CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC ACCTCTGTCT 
CCGACATCCA CGCCTTTGGT CATCACCACA GCTGTGACCT TTGGTAGCAA TGGCACCATC 
CTAGAGGAGG GCCTGGACCC TGACAATGGA ATTGAACTGG AGACCCTGGA GACTGAGTTA 
GCCAGAGAAG ATGAAGAAGA AGATGGTCCC ACCATGGGCC CCCTCTTCCG GGCAGCAGAA 
CAGTCATGGA ATACTGAATT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA 
GAACCAGCCG TGGTTGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCAGGACCC 
CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGGCCCCCAG GATTCCCTGG AGACCGTGGT 
CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATTG ATGGGATCCG GGGACTCCCA 
GGCACTGTGA TCATGATACC GTTCCAGTTT GCAGGAGGCT CTCACAAAGG ACCCCCAGTA 
TCCTTCCTGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTCTC TATGAAAGGC 
CCTCCTGGCC CGGTGGGGCT CACTGGACGC CCAGGTCCCG TGGGCCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCTCAGGGTC CCCGAGGGCT TCAGGGACCT 
CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCCCTG GAGCAGATGG GGCTCGGGGC 
CTCCCAGGCA CCACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT 
GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAA 
GATGGGCGGA AGGGAGTAGA GGGACCTCCG GGGCCCACTG GCCAGGCGGG GGAGCCGGGC 
CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACACCC GGGTGTGACT 
GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC 
CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCTG GCCCCCAGGG GCCCATCGGC 
ACTCCTGGGG AGAAGGGTCC CCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA 
GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA 
ACAGGGTCAG CAGGCCCTCC GGGCTATCCG GGACCTCGGG GTGTGAAGGG TACTTCTGGC 
AACCGAGGCC TCCAGGGGGA GAAAGGAGAG AGGGGAGAGG ATGGTTTCCC AGGCTTCAAG 
GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCTGGAC CTCCAGGTCC TCGGGGAGAG 
GATGGTCCTG AAGGGCTCAA GGGGCAGGAG GGGCTGGTTG GCGAGGAGGG TCCCCCAGGC 
TCGGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTTC CAGGTTATCC AGGACGTCCA 
GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCCCTGGGGC CATTAGGAGA AAAAGGGAAG 
CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA 
GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCTA AGGGTGATGT GGGCCAGGAT 
GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TGCAAGGTCC TCCTGGGTTC 
TCCGGACCAA AGGGTCCCCC TGGTCCCCAG GGGAAAGATG GGCTACCTGG ACACCCTGGA 
CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGAGTCGTG 
GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC TCCAGGGCCC 
CCTGGACCTC CAGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GACGTGGGAC CACTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT 
GGTCCTCATG GAGCCCCCGG GGAACCAGGA CCAACTGGTT TAAAGGGTGA CAAGGGCCCC 
CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG 
GGCATTGGGC TTCCTGGTCA AAGTGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG 
GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCCACTGGTA AAGATGGCAT CCCCGGGCCC 
CTGGGCCTTC CTGGACCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA 
GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGAGACA AGGGAGATGC GGGCCCGCCT 
GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCAGGCT CTCCAGGAGC AGATGGGGCT 
CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGGGGC 
TTCCCTGGCA CGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG 
GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCATGGAG CTCCAGGCCC TCGGGGCCCC 
CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCCGGC 
GCTGTGGGTG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC AGGGACCCCA 
GGCGCCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT 
GCTGGACCCC CAGGCAAGAA AGGCCCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC 
CCCGCAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGGT 
GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT 
TCTGGGGAGC CTGGTGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT 
CGAGAAGGCA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCCGA TGGACCCCCA 
GGGAGGACAG GCCCAGTGGG GGCTGTAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT 
CGTGGGATCC CTGGACCTGT GGGTGAACCA GGCCTCCTGG GACCTCCTGG ACTGCTAGGC 
CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG 
GGAGAAAAGG GCCACATTGG ATTAACTGGT CTCATCGGCC CCCCTGGGGA GGCTGGTGAG 
AAAGGGGATC AGGGGTTGCC AGGAGTCCAA GGCCCCCCTG GCCTCCAGGG AGAACCGGGT 
TCCCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA 
GGACAGAAAG GCTCAAAGGG GTCCCCAGGA ACCCCAGGTC CTCGTGGAGA CACCGGCCCC 
CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ACGGGCTGCG CCGGCGCCGA 
CGTTCCCTGC CAGGCTTGGG GACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG 
GCGTCGCTGA ACTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG 
GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCACATC TGCCGGATGG GGAATACTGG 
ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCACTCAGGG TTTTCTGTAA CTTCACGGCA 
GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CAGTGAAACT GGCCTCTTGG 
TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC 
GTGGATGCCG ATGGGGCTCC GGTGAGTGTG GTACAGCTGA CCTTCCTGAA GCTGCTGAGT 
GCCACGGCCC GCCAGAGCTT CACGTTCACC TGCCAGAACT CGGCCGCGTG GCTGGACGAA 
GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGCTGTCC 
TTCAACCAGA CGGCAGCCGC CACCATCACG GTCCCCTACG ATGGCTGCAG GCTCCGGAAG 
GGACAGTCGA AGACCCTCTT TGAGTTCAAC TCCTCCCGAG TGGGATTTCT GCCCTTGTTG 
GATGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT 
GTCTGCTTTA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_012135957.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011991347.1 Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOv17335
Clone ID Related Accession (Same CDS sequence)	XM_012135957.2
Accession Version	XM_012135957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	collagen alpha-3(V) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942413.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012135957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGCA GATCCTGTGG ATGTGCTGAA GGCGCTGGGC 
ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGCCCCGGCC TCTGCCCCCA GAGGGCCCCA 
GAGGGTGACC GAGCATTCCG GGTGGGCAAG ACCAGCACAC TTGGTGTCCC CACGCAAGAG 
CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG 
CCAACCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GTGGCGTCCG GCAGCTGGGC 
TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG 
GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG 
ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCGTGTTGG CTCAGGGACC TCGATTCATC 
AGTACAGCGG GACTCACTGT GCTAGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA 
GATGTCCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCCG CCTTCCAGGC ATGTGAGCGG 
TACCTTCCTG GCTGTGATGA CCTGGATCCT ACAGTCACCA GGGCCCCTCA GGGGGAGCCA 
GAAACCCCTC CCGCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAGAAAGG GCGAGGTCGT 
AAGGGGAAGG GCAGGAAGAA GAAGCTCAAG GAAGCTTTGG CTGCGAGTCC GCCTCCTGGG 
CCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCACCTCTG 
TCTCCGACAT CCACGCCTTT GGTCATCACC ACAGCTGTGA CCTTTGGTAG CAATGGCACC 
ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAATTGAAC TGGAGACCCT GGAGACTGAG 
TTAGCCAGAG AAGATGAAGA AGAAGATGGT CCCACCATGG GCCCCCTCTT CCGGGCAGCA 
GAACAGTCAT GGAATACTGA ATTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA 
GGAGAACCAG CCGTGGTTGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCAGGA 
CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGGCCCC CAGGATTCCC TGGAGACCGT 
GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TTGATGGGAT CCGGGGACTC 
CCAGGCACTG TGATCATGAT ACCGTTCCAG TTTGCAGGAG GCTCTCACAA AGGACCCCCA 
GTATCCTTCC TGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT CTCTATGAAA 
GGCCCTCCTG GCCCGGTGGG GCTCACTGGA CGCCCAGGTC CCGTGGGCCT CCCTGGGTAT 
CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCTCAGG GTCCCCGAGG GCTTCAGGGA 
CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCC CTGGAGCAGA TGGGGCTCGG 
GGCCTCCCAG GCACCACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG 
CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA 
GAAGATGGGC GGAAGGGAGT AGAGGGACCT CCGGGGCCCA CTGGCCAGGC GGGGGAGCCG 
GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACA CCCGGGTGTG 
ACTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC 
CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CTGGCCCCCA GGGGCCCATC 
GGCACTCCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TTCCAGGTTA TCCAGGACGT 
CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCCCTGG GGCCATTAGG AGAAAAAGGG 
AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT 
GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CTAAGGGTGA TGTGGGCCAG 
GATGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG 
TTCTCCGGAC CAAAGGGTCC CCCTGGTCCC CAGGGGAAAG ATGGGCTACC TGGACACCCT 
GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGAGTC 
GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCTCCAGGG 
CCCCCTGGAC CTCCAGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG 
GGGGACGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC 
CCTGGTCCTC ATGGAGCCCC CGGGGAACCA GGACCAACTG GTTTAAAGGG TGACAAGGGC 
CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA 
GGGGGCATTG GGCTTCCTGG TCAAAGTGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG 
AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCCACTG GTAAAGATGG CATCCCCGGG 
CCCCTGGGCC TTCCTGGACC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG 
GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGAG ACAAGGGAGA TGCGGGCCCG 
CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCAG GCTCTCCAGG AGCAGATGGG 
GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGG 
GGCTTCCCTG GCACGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA 
AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCATG GAGCTCCAGG CCCTCGGGGC 
CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCC 
GGCGCTGTGG GTGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCAGGGACC 
CCAGGCGCCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG 
GCTGCTGGAC CCCCAGGCAA GAAAGGCCCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG 
GGCCCCGCAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA 
GGTGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC 
GCTTCTGGGG AGCCTGGTGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG 
GGTCGAGAAG GCAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC CGATGGACCC 
CCAGGGAGGA CAGGCCCAGT GGGGGCTGTA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT 
CTTCGTGGGA TCCCTGGACC TGTGGGTGAA CCAGGCCTCC TGGGACCTCC TGGACTGCTA 
GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC 
AAGGGAGAAA AGGGCCACAT TGGATTAACT GGTCTCATCG GCCCCCCTGG GGAGGCTGGT 
GAGAAAGGGG ATCAGGGGTT GCCAGGAGTC CAAGGCCCCC CTGGCCTCCA GGGAGAACCG 
GGTTCCCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT 
CCAGGACAGA AAGGCTCAAA GGGGTCCCCA GGAACCCCAG GTCCTCGTGG AGACACCGGC 
CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC 
CGACGTTCCC TGCCAGGCTT GGGGACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
ATGGCGTCGC TGAACTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC 
CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCAC ATCTGCCGGA TGGGGAATAC 
TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCACTCA GGGTTTTCTG TAACTTCACG 
GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACAGTGAA ACTGGCCTCT 
TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC 
TACGTGGATG CCGATGGGGC TCCGGTGAGT GTGGTACAGC TGACCTTCCT GAAGCTGCTG 
AGTGCCACGG CCCGCCAGAG CTTCACGTTC ACCTGCCAGA ACTCGGCCGC GTGGCTGGAC 
GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGCTG 
TCCTTCAACC AGACGGCAGC CGCCACCATC ACGGTCCCCT ACGATGGCTG CAGGCTCCGG 
AAGGGACAGT CGAAGACCCT CTTTGAGTTC AACTCCTCCC GAGTGGGATT TCTGCCCTTG 
TTGGATGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC 
CCTGTCTGCT TTAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011991347.1
CDS	172..5052
Translation	MGSRRGPGQPRAGLCLLLASLQLLPRTQAADPVDVLKALGMRGGQAGIPEGPGLCPQRAPEGDRAFRVGKTSTLGVPTQELFQDGHFPENFSILITLRGQPTNRSVLLSIYDEGGVRQLGLALGPALDLLGDSFSPLPQQVNLTDGRWHRVAVSVDGGMVTLVADCEPQLPVLAQGPRFISTAGLTVLGTQDLGEETFEGDVQELVISPDPQAAFQACERYLPGCDDLDPTVTRAPQGEPETPPARRKGKGKGKKKGRGRKGKGRKKKLKEALAASPPPGPLDNQTSTDIPKTETPEPPLSPTSTPLVITTAVTFGSNGTILEEGLDPDNGIELETLETELAREDEEEDGPTMGPLFRAAEQSWNTEFQIFPGAGEKGEKGEPAVVEQGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGIRGLPGTVIMIPFQFAGGSHKGPPVSFLQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGEMGEMGPQGPRGLQGPHGPPGREGKMGRPGADGARGLPGTTGPKGDRGFDGLPGLPGEKGQRGDFGYVGKPGPPGEDGRKGVEGPPGPTGQAGEPGPRGLIGPRGSPGPLGHPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNPGSQGIPGPQGPIGTPGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGLEGERGPPGARGERGQPGATGQPGPKGDVGQDGAPGFPGEKGLPGLQGPPGFSGPKGPPGPQGKDGLPGHPGQRGELGFQGQTGPPGPAGVVGPQGKTGEAGPLGERGPPGPPGPPGEQGLPGLEGREGAKGDVGPLGPLGKEGPPGPRGFPGPHGAPGEPGPTGLKGDKGPPGPVGANGSPGERGPVGPAGGIGLPGQSGGQGPVGPTGEKGSPGERGAPGPTGKDGIPGPLGLPGPPGAAGPSGEEGDKGEVGAPGHKGSKGDKGDAGPPGPTGIRGLVGHPGSPGADGAQGRRGPPGLFGQKGDDGVRGFPGTIGPPGLQGMPGPAGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGPPGLPGGVGQPGAVGEKGEPGEPGDPGPPGTPGAPGPRGEVGEKGDTGPSGAAGPPGKKGPPGEDGAKGNVGPAGLPGDLGPPGDPGVSGQGGPPGEKGNPGDVGGPGPPGASGEPGAPGPPGKRGPSGRMGREGREGEKGAKGEPGPDGPPGRTGPVGAVGPPGRVGPEGLRGIPGPVGEPGLLGPPGLLGPPGPLGPSGLPGLKGDAGPKGEKGHIGLTGLIGPPGEAGEKGDQGLPGVQGPPGLQGEPGSPGPIGSLGHPGPQGVVGPPGQKGSKGSPGTPGPRGDTGPPGPPGPPGPSAELHGLRRRRRSLPGLGTPEGGLEEVLASMASLNFELEQLRRPPGTAERPGLVCKELHRSHPHLPDGEYWIDPNQGCARDALRVFCNFTAGGETCLYPDKKFETVKLASWSKEKPGGWYSTFRRGKKFSYVDADGAPVSVVQLTFLKLLSATARQSFTFTCQNSAAWLDEAAGDHSRSARFLGANGEELSFNQTAAATITVPYDGCRLRKGQSKTLFEFNSSRVGFLPLLDVAAADFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_012135957.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon collagen, type V, alpha 3 (COL5A3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012135957.2

ORF Insert Sequence:

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGCA GATCCTGTGG ATGTGCTGAA GGCGCTGGGC 
ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGCCCCGGCC TCTGCCCCCA GAGGGCCCCA 
GAGGGTGACC GAGCATTCCG GGTGGGCAAG ACCAGCACAC TTGGTGTCCC CACGCAAGAG 
CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG 
CCAACCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GTGGCGTCCG GCAGCTGGGC 
TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG 
GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG 
ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCGTGTTGG CTCAGGGACC TCGATTCATC 
AGTACAGCGG GACTCACTGT GCTAGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA 
GATGTCCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCCG CCTTCCAGGC ATGTGAGCGG 
TACCTTCCTG GCTGTGATGA CCTGGATCCT ACAGTCACCA GGGCCCCTCA GGGGGAGCCA 
GAAACCCCTC CCGCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAGAAAGG GCGAGGTCGT 
AAGGGGAAGG GCAGGAAGAA GAAGCTCAAG GAAGCTTTGG CTGCGAGTCC GCCTCCTGGG 
CCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCACCTCTG 
TCTCCGACAT CCACGCCTTT GGTCATCACC ACAGCTGTGA CCTTTGGTAG CAATGGCACC 
ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAATTGAAC TGGAGACCCT GGAGACTGAG 
TTAGCCAGAG AAGATGAAGA AGAAGATGGT CCCACCATGG GCCCCCTCTT CCGGGCAGCA 
GAACAGTCAT GGAATACTGA ATTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA 
GGAGAACCAG CCGTGGTTGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCAGGA 
CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGGCCCC CAGGATTCCC TGGAGACCGT 
GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TTGATGGGAT CCGGGGACTC 
CCAGGCACTG TGATCATGAT ACCGTTCCAG TTTGCAGGAG GCTCTCACAA AGGACCCCCA 
GTATCCTTCC TGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT CTCTATGAAA 
GGCCCTCCTG GCCCGGTGGG GCTCACTGGA CGCCCAGGTC CCGTGGGCCT CCCTGGGTAT 
CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCTCAGG GTCCCCGAGG GCTTCAGGGA 
CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCC CTGGAGCAGA TGGGGCTCGG 
GGCCTCCCAG GCACCACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG 
CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA 
GAAGATGGGC GGAAGGGAGT AGAGGGACCT CCGGGGCCCA CTGGCCAGGC GGGGGAGCCG 
GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACA CCCGGGTGTG 
ACTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC 
CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CTGGCCCCCA GGGGCCCATC 
GGCACTCCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TTCCAGGTTA TCCAGGACGT 
CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCCCTGG GGCCATTAGG AGAAAAAGGG 
AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT 
GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CTAAGGGTGA TGTGGGCCAG 
GATGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTGCAAGG TCCTCCTGGG 
TTCTCCGGAC CAAAGGGTCC CCCTGGTCCC CAGGGGAAAG ATGGGCTACC TGGACACCCT 
GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGAGTC 
GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCTCCAGGG 
CCCCCTGGAC CTCCAGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG 
GGGGACGTGG GACCACTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC 
CCTGGTCCTC ATGGAGCCCC CGGGGAACCA GGACCAACTG GTTTAAAGGG TGACAAGGGC 
CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA 
GGGGGCATTG GGCTTCCTGG TCAAAGTGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG 
AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCCACTG GTAAAGATGG CATCCCCGGG 
CCCCTGGGCC TTCCTGGACC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG 
GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGAG ACAAGGGAGA TGCGGGCCCG 
CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCAG GCTCTCCAGG AGCAGATGGG 
GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGG 
GGCTTCCCTG GCACGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA 
AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCATG GAGCTCCAGG CCCTCGGGGC 
CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCC 
GGCGCTGTGG GTGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCAGGGACC 
CCAGGCGCCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG 
GCTGCTGGAC CCCCAGGCAA GAAAGGCCCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG 
GGCCCCGCAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA 
GGTGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC 
GCTTCTGGGG AGCCTGGTGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG 
GGTCGAGAAG GCAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC CGATGGACCC 
CCAGGGAGGA CAGGCCCAGT GGGGGCTGTA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT 
CTTCGTGGGA TCCCTGGACC TGTGGGTGAA CCAGGCCTCC TGGGACCTCC TGGACTGCTA 
GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC 
AAGGGAGAAA AGGGCCACAT TGGATTAACT GGTCTCATCG GCCCCCCTGG GGAGGCTGGT 
GAGAAAGGGG ATCAGGGGTT GCCAGGAGTC CAAGGCCCCC CTGGCCTCCA GGGAGAACCG 
GGTTCCCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT 
CCAGGACAGA AAGGCTCAAA GGGGTCCCCA GGAACCCCAG GTCCTCGTGG AGACACCGGC 
CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCACGGGCT GCGCCGGCGC 
CGACGTTCCC TGCCAGGCTT GGGGACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
ATGGCGTCGC TGAACTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC 
CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCAC ATCTGCCGGA TGGGGAATAC 
TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCACTCA GGGTTTTCTG TAACTTCACG 
GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACAGTGAA ACTGGCCTCT 
TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC 
TACGTGGATG CCGATGGGGC TCCGGTGAGT GTGGTACAGC TGACCTTCCT GAAGCTGCTG 
AGTGCCACGG CCCGCCAGAG CTTCACGTTC ACCTGCCAGA ACTCGGCCGC GTGGCTGGAC 
GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGCTG 
TCCTTCAACC AGACGGCAGC CGCCACCATC ACGGTCCCCT ACGATGGCTG CAGGCTCCGG 
AAGGGACAGT CGAAGACCCT CTTTGAGTTC AACTCCTCCC GAGTGGGATT TCTGCCCTTG 
TTGGATGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC 
CCTGTCTGCT TTAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_004009289.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004009338.2 Ovis aries collagen, type V, alpha 3 (COL5A3), transcript variant X1, mRNA.

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CloneID	OSm49458
Clone ID Related Accession (Same CDS sequence)	XM_004009289.3
Accession Version	XM_004009289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	collagen alpha-3(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004009289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGAGGTTG CAGTTTTGGA AGCCACTAAG CTCTTCGACA GAGCCTGTTT GGAGAAAAAG 
TCTGGGACCC CGGAATTTAA GGCTAGCTGG CCAATCAGCG CTCAGCACAA ACTTTCCCAA 
TTCCCCACAC CAGCTGTCAG CTGCCCCCCC TCCCTTGAGC TCCGCCTGGC CGGGCGCTGG 
AGCGCAGGGC GAGGCCAGGG CGGGGTCTCG GGCAGTCTCC TGCGGATCCC GATGTTGGGC 
CCCGCGCCGG GGCCCGGCCT CTCCCGGCCC CTGCCCTCTC TGCAGCTTCT GCCCCGGACG 
CAGGCCGATC CTGTGGATGT GCTGAAGGCG CTGGGCATGC GGGGGGGCCA GGCTGGGATC 
CCTGAGGGCC CCGGCCTCTG CCCCCAGAGG GCCCCAGAGG GTGACCGAGC ATTCCGGGTG 
GGCAAGACCA GCACACTTGG TGTCCCCACG CAAGAGCTCT TCCAAGATGG GCACTTTCCT 
GAGAACTTCT CCATCCTGAT CACCCTGCGG GGCCAGCCAA CCAACCGTTC TGTCCTTCTG 
TCCATCTATG ATGAGGGTGG CGTCCGGCAG CTGGGCTTGG CCTTGGGGCC AGCTCTGGAC 
CTCCTAGGTG ACTCCTTCAG CCCCCTCCCC CAGCAGGTCA ACCTCACGGA TGGCAGGTGG 
CACCGTGTGG CAGTCAGTGT GGACGGTGGG ATGGTGACCC TGGTGGCTGA TTGTGAACCT 
CAGCTCCCCG TGTTGGCTCA GGGACCTCGA TTCATCAGTA CAGCGGGACT CACTGTGCTG 
GGGACCCAGG ACCTTGGCGA GGAGACTTTT GAGGGAGATG TCCAGGAGCT GGTGATAAGC 
CCAGATCCCC AGGCCGCCTT CCAGGCATGT GAGCGGTACC TTCCTGGCTG TGATGACCTG 
GATCCTACAG TCACCAGGGC CCCTCAGGGG GAGCCAGAAA CCCCTCCCCC TAGGCGGAAG 
GGGAAAGGAA AAGGGAAGAA GAAAGGGCGA GGTCGTAAGG GGAAGGGCAG GAAGAAGAAG 
CTCAAGGAAG CTTTGGCTGC GAGTCTGCCT CCTGGGCCCC TGGACAACCA GACCTCCACT 
GACATCCCCA AGACAGAGAC ACCAGAACCA CCTCTGTCTC CGACATCCAC GCCTTTGGTC 
ATCACCACAG CTGTGACCTT TGGTAGCAAT GGCACCATCC TAGAGGAGGG CCTGGACCCT 
GACAATGGAA TTGAACTGGA GACCCTGGAG ACTGAGTTAG CCAGAGAAGA TGAAGAAGAA 
GATGGTCCCA CCATGGGCCC CCTCTTCCGG GCAGCAGAAC AGTCATGGAA TACTGAATTC 
CAGATCTTTC CTGGTGCTGG GGAGAAAGGA GAAAAAGGAG AGCCAGCCGT GGTTGAACAG 
GGACAGCAGT TTGAAGGACC CCCAGGAGCC CCAGGACCCC AAGGGGTGGT TGGCCCCTCA 
GGCCCTCCTG GGCCCCCAGG ATTCCCTGGA GACCGTGGTC TACCGGGCCC TGCTGGCCTC 
CCAGGCATCC CTGGCATTGA TGGGATCCGG GGACTCCCAG GCACTGTGAT CATGATACCG 
TTCCAGTTTG CAGGAGGCTC TCACAAAGGA CCCCCAGTAT CCTTCCAGCA GGCCCAGGCT 
CAGGCAGTCC TGCAACAAGC TCAGCTCTCT ATGAAAGGCC CTCCTGGCCC GGTGGGGCTC 
ACTGGACGCC CAGGTCCCGT GGGCCTCCCT GGGTATCCAG GTCTGAAAGG AGAGATGGGA 
GAAATGGGGC CTCAGGGTCC CCGAGGGCTT CAGGGACCTC ATGGGCCCCC TGGCCGAGAG 
GGAAAGATGG GCCGCCCTGG AGCAGATGGG GCTCGGGGCC TCCCAGGCAC CACGGGACCT 
AAGGGTGATC GGGGCTTTGA TGGCCTGCCA GGGCTGCCTG GTGAGAAGGG CCAAAGGGGT 
GACTTTGGTT ATGTGGGGAA ACCTGGTCCC CCAGGAGAAG ATGGGCGGAA GGGAGTAGAG 
GGACCTCCGG GGCCCACTGG CCAGGCGGGG GAGCCGGGCC CCCGAGGACT GATTGGCCCT 
AGAGGCTCCC CCGGACCCCT GGGACGCCCG GGTGTGACTG GAAGTGATGG TGCTCCAGGT 
GCCAAAGGAA ATGTGGGTCC TCCAGGAGAA CCAGGCCCCC CAGGACAGCA GGGAAATCCT 
GGGTCCCAGG GAATTCCTGG CCCCCAGGGG CCCATCGGCA CTCCTGGGGA GAAGGGTCCC 
CCTGGAAACC CAGGAATTCC AGGCCTCCCA GGATCTGAAG GCCCTCCGGG TCACCCAGGT 
CATGAGGGCC CAACAGGAGA GAAAGGGGCC CAGGGTCCAA CAGGGTCAGC AGGCCCTCCA 
GGCTATCCGG GACCTCGGGG TGTGAAGGGT ACTTCTGGCA ACCGAGGCCT CCAGGGGGAG 
AAAGGAGAGA GGGGAGAGGA TGGTTTCCCA GGCTTCAAGG GTGATGCGGG GCTTAAAGGA 
GATCGGGGGC AACCCGGACC TCCAGGTCCT CGGGGAGAGG ATGGTCCTGA AGGGCTCAAG 
GGGCAGGAGG GGCTGGTTGG CGAGGAGGGT CCCCCAGGCT CGGCTGGGGA GAAGGGCAAG 
CTTGGCGTGC CGGGTCTTCC AGGTTATCCA GGACGTCCAG GCCCTAAGGG ATCTATTGGC 
TTTCCGGGAC CCCTGGGACC ATTAGGAGAA AAAGGGAAGC GGGGGAAGGC TGGGCAGCCA 
GGCCTGGAAG GAGAGCGGGG ACCACCAGGT GCCCGTGGAG AGAGAGGGCA ACCAGGTGCC 
ACAGGGCAGC CGGGCCCTAA GGGTGATGTG GGCCAGGATG GAGCCCCTGG GTTCCCTGGA 
GAAAAGGGCC TCCCAGGTCT ACAAGGTCCT CCTGGGTTCT CCGGACCAAA GGGTCCCCCT 
GGTCCCCAGG GGAAAGATGG GCTACCTGGA CACCCTGGAC AGAGAGGAGA ATTGGGTTTC 
CAAGGTCAGA CAGGCCCACC TGGACCAGCT GGAGTTGTGG GTCCTCAGGG AAAGACAGGA 
GAAGCAGGGC CTCTGGGTGA GAGGGGGCCT CCAGGGCCCC CTGGACCTCC AGGTGAACAA 
GGTCTTCCGG GCCTGGAAGG CAGAGAGGGG GCCAAGGGGG ACGTGGGACC ATTGGGACCC 
CTTGGGAAGG AAGGGCCACC TGGACCCAGA GGTTTCCCTG GTCCTCATGG AGCCCCCGGG 
GAACCAGGAC CAACTGGTTT AAAGGGTGAC AAGGGCCCCC CAGGTCCTGT TGGTGCCAAC 
GGGCCCCCCG GGGGGGGGGG CCCTGTAGGC CCAGCAGGGG GCATTGGGCT TCCTGGTCAA 
AGTGGAGGCC AAGGCCCCGT GGGCCCCACA GGCGAGAAGG GTTCCCCGGG TGAACGTGGC 
GCCCCTGGCC CCACTGGTAA AGATGGCATC CCCGGGCCCC TGGGCCTTCC TGGACCCCCT 
GGAGCTGCGG GGGCTTCTGG GGGGGAAGGG GGCAAGGGGA GAGGGGGGAT AGAAGTGGGT 
GCCCCCGGTC ACAAAGGCAG CAAAGGAGAC AAGGGAGATG CGGGCCCGCC TGGACCAACA 
GGGATACGGG GTCTGGTGGG ACACCCAGGC TCTCCAGGAG CAGATGGGGC TCAGGGACGC 
CGGGGACCCC CAGGCCTCTT TGGGCAGAAA GGAGATGATG GAGTGAGGGG CTTCCCTGGC 
ACGATTGGTC CTCCTGGCCT ACAGGGAATG CCAGGCCCTG CTGGAGAAAA GGGAGAAGTT 
GGAGATGTAG GGTCCATGGG TCCCCATGGA GCTCCAGGCC CTCGGGGCCC CCAGGGCCCC 
AGCGGATCAG AGGGTCCTCC AGGGTTGCCT GGGGGAGTTG GTCAGCCCGG CGCTGTGGGT 
GAGAAGGGTG AGCCAGGAGA GCCTGGAGAC CCAGGACCCC CAGGGACCCC AGGCGCCCCG 
GGCCCCAGGG GAGAAGTTGG TGAAAAGGGG GATACAGGCC CATCTGGGGC TGCTGGACCC 
CCAGGCAAGA AAGGCCCCCC TGGAGAGGAT GGAGCAAAGG GGAATGTGGG CCCCGCAGGC 
CTCCCAGGAG ACCTAGGCCC CCCCGGAGAC CCTGGAGTTT CGGGTCAAGG TGGCCCCCCA 
GGGGAGAAGG GAAACCCTGG AGATGTGGGG GGACCGGGTC CACCTGGCGC TTCTGGGGAG 
CCTGGTGCCC CTGGGCCTCC TGGTAAAAGG GGTCCTTCTG GTCGCATGGG TCGAGAAGGC 
AGAGAAGGAG AGAAAGGTGC CAAGGGGGAA CCAGGTCCCG ATGGACCCCC AGGGAGGACA 
GGCCCAGTGG GGGCTGTAGG GCCCCCTGGA CGTGTTGGAC CTGAGGGTCT TCGTGGGATC 
CCTGGACCTG TGGGTGAACC AGGCCTCCTG GGACCTCCTG GACTGCTAGG CCCTCCTGGT 
CCCCTGGGGC CCTCTGGCCT ACCAGGGCTG AAGGGAGATG CTGGCCCCAA GGGAGAAAAG 
GGCCACATTG GATTAACTGG TCTCATCGGC CCCCCTGGGG AGGCTGGTGA GAAAGGGGAT 
CAGGGGTTGC CAGGAGTCCA AGGCCCCCCT GGCCTCCAGG GAGAACCGGG TTCCCCTGGC 
CCCATTGGCT CTCTGGGCCA CCCTGGGCCC CAAGGCGTGG TGGGTCCTCC AGGACAGAAA 
GGCTCAAAGG GGTCCCCAGG AACCCAAGGT CCTCGTGGAG ACACCGGCCC CCCAGGTCCT 
CCTGGTCCCC CGGGTCCCTC TGCGGAGCTG CATGGGCTGC GCCGGCGCCG ACGTTCCCTG 
CCAGGCTTGG GGACCCCGGA GGGCGGCCTG GAGGAGGTGC TGGCCTCGAT GGCGTCGCTG 
AGCTTCGAGT TAGAGCAGCT GCGGCGCCCT CCCGGCACGG CTGAGCGCCC GGGCCTCGTG 
TGCAAAGAGT TGCACCGCAG CCATCCACAT CCGCCGGATG GGGAATACTG GATTGATCCC 
AATCAAGGCT GCGCGCGGGA TGCACTCAGG GTTTTCTGTA ACTTCACGGC AGGAGGAGAA 
ACCTGCCTCT ACCCAGACAA GAAGTTTGAG ACAGTGAAAC TGGCCTCTTG GTCCAAAGAA 
AAGCCAGGTG GCTGGTATAG CACATTCCGT CGAGGGAAAA AGTTCTCCTA CGTGGATGCC 
GATGGGGCCC CGGTGAGTGT GGTACAGCTG ACCTTCCTGA AGCTGCTGAG TGCCACGGCC 
CGCCAGAGCT TCACGTTCAC CTGCCAGAAC TCGGCCGCCT GGCTGGACGA AGCTGCAGGT 
GACCACAGCC GCTCTGCCCG TTTCCTCGGG GCCAATGGGG AGGAGCTGTC CTTCAACCAG 
ACAGCAGCCG CCACCATCAC GGTCCCCTAC GATGGCTGCA GGCTCCGGAA GGGACAGTCG 
AAGACCCTCT TTGAGTTCAA CTCCTCCCGA GTGGGATTTC TGCCCTTGTT GGATGTGGCA 
GCTGCTGACT TTGGCCAGAC GAACCAGAAG TTTGGGTTTG AACTGGGCCC TGTCTGCTTT 
AGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004009338.2
CDS	1..5469
Translation	MEVAVLEATKLFDRACLEKKSGTPEFKASWPISAQHKLSQFPTPAVSCPPSLELRLAGRWSAGRGQGGVSGSLLRIPMLGPAPGPGLSRPLPSLQLLPRTQADPVDVLKALGMRGGQAGIPEGPGLCPQRAPEGDRAFRVGKTSTLGVPTQELFQDGHFPENFSILITLRGQPTNRSVLLSIYDEGGVRQLGLALGPALDLLGDSFSPLPQQVNLTDGRWHRVAVSVDGGMVTLVADCEPQLPVLAQGPRFISTAGLTVLGTQDLGEETFEGDVQELVISPDPQAAFQACERYLPGCDDLDPTVTRAPQGEPETPPPRRKGKGKGKKKGRGRKGKGRKKKLKEALAASLPPGPLDNQTSTDIPKTETPEPPLSPTSTPLVITTAVTFGSNGTILEEGLDPDNGIELETLETELAREDEEEDGPTMGPLFRAAEQSWNTEFQIFPGAGEKGEKGEPAVVEQGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGIRGLPGTVIMIPFQFAGGSHKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGEMGEMGPQGPRGLQGPHGPPGREGKMGRPGADGARGLPGTTGPKGDRGFDGLPGLPGEKGQRGDFGYVGKPGPPGEDGRKGVEGPPGPTGQAGEPGPRGLIGPRGSPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNPGSQGIPGPQGPIGTPGEKGPPGNPGIPGLPGSEGPPGHPGHEGPTGEKGAQGPTGSAGPPGYPGPRGVKGTSGNRGLQGEKGERGEDGFPGFKGDAGLKGDRGQPGPPGPRGEDGPEGLKGQEGLVGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGLEGERGPPGARGERGQPGATGQPGPKGDVGQDGAPGFPGEKGLPGLQGPPGFSGPKGPPGPQGKDGLPGHPGQRGELGFQGQTGPPGPAGVVGPQGKTGEAGPLGERGPPGPPGPPGEQGLPGLEGREGAKGDVGPLGPLGKEGPPGPRGFPGPHGAPGEPGPTGLKGDKGPPGPVGANGPPGGGGPVGPAGGIGLPGQSGGQGPVGPTGEKGSPGERGAPGPTGKDGIPGPLGLPGPPGAAGASGGEGGKGRGGIEVGAPGHKGSKGDKGDAGPPGPTGIRGLVGHPGSPGADGAQGRRGPPGLFGQKGDDGVRGFPGTIGPPGLQGMPGPAGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGPPGLPGGVGQPGAVGEKGEPGEPGDPGPPGTPGAPGPRGEVGEKGDTGPSGAAGPPGKKGPPGEDGAKGNVGPAGLPGDLGPPGDPGVSGQGGPPGEKGNPGDVGGPGPPGASGEPGAPGPPGKRGPSGRMGREGREGEKGAKGEPGPDGPPGRTGPVGAVGPPGRVGPEGLRGIPGPVGEPGLLGPPGLLGPPGPLGPSGLPGLKGDAGPKGEKGHIGLTGLIGPPGEAGEKGDQGLPGVQGPPGLQGEPGSPGPIGSLGHPGPQGVVGPPGQKGSKGSPGTQGPRGDTGPPGPPGPPGPSAELHGLRRRRRSLPGLGTPEGGLEEVLASMASLSFELEQLRRPPGTAERPGLVCKELHRSHPHPPDGEYWIDPNQGCARDALRVFCNFTAGGETCLYPDKKFETVKLASWSKEKPGGWYSTFRRGKKFSYVDADGAPVSVVQLTFLKLLSATARQSFTFTCQNSAAWLDEAAGDHSRSARFLGANGEELSFNQTAAATITVPYDGCRLRKGQSKTLFEFNSSRVGFLPLLDVAAADFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_004009289.3
Organism	Ovis aries(sheep)
Definition	Ovis aries collagen, type V, alpha 3 (COL5A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004009289.3

ORF Insert Sequence:

ATGGAGGTTG CAGTTTTGGA AGCCACTAAG CTCTTCGACA GAGCCTGTTT GGAGAAAAAG 
TCTGGGACCC CGGAATTTAA GGCTAGCTGG CCAATCAGCG CTCAGCACAA ACTTTCCCAA 
TTCCCCACAC CAGCTGTCAG CTGCCCCCCC TCCCTTGAGC TCCGCCTGGC CGGGCGCTGG 
AGCGCAGGGC GAGGCCAGGG CGGGGTCTCG GGCAGTCTCC TGCGGATCCC GATGTTGGGC 
CCCGCGCCGG GGCCCGGCCT CTCCCGGCCC CTGCCCTCTC TGCAGCTTCT GCCCCGGACG 
CAGGCCGATC CTGTGGATGT GCTGAAGGCG CTGGGCATGC GGGGGGGCCA GGCTGGGATC 
CCTGAGGGCC CCGGCCTCTG CCCCCAGAGG GCCCCAGAGG GTGACCGAGC ATTCCGGGTG 
GGCAAGACCA GCACACTTGG TGTCCCCACG CAAGAGCTCT TCCAAGATGG GCACTTTCCT 
GAGAACTTCT CCATCCTGAT CACCCTGCGG GGCCAGCCAA CCAACCGTTC TGTCCTTCTG 
TCCATCTATG ATGAGGGTGG CGTCCGGCAG CTGGGCTTGG CCTTGGGGCC AGCTCTGGAC 
CTCCTAGGTG ACTCCTTCAG CCCCCTCCCC CAGCAGGTCA ACCTCACGGA TGGCAGGTGG 
CACCGTGTGG CAGTCAGTGT GGACGGTGGG ATGGTGACCC TGGTGGCTGA TTGTGAACCT 
CAGCTCCCCG TGTTGGCTCA GGGACCTCGA TTCATCAGTA CAGCGGGACT CACTGTGCTG 
GGGACCCAGG ACCTTGGCGA GGAGACTTTT GAGGGAGATG TCCAGGAGCT GGTGATAAGC 
CCAGATCCCC AGGCCGCCTT CCAGGCATGT GAGCGGTACC TTCCTGGCTG TGATGACCTG 
GATCCTACAG TCACCAGGGC CCCTCAGGGG GAGCCAGAAA CCCCTCCCCC TAGGCGGAAG 
GGGAAAGGAA AAGGGAAGAA GAAAGGGCGA GGTCGTAAGG GGAAGGGCAG GAAGAAGAAG 
CTCAAGGAAG CTTTGGCTGC GAGTCTGCCT CCTGGGCCCC TGGACAACCA GACCTCCACT 
GACATCCCCA AGACAGAGAC ACCAGAACCA CCTCTGTCTC CGACATCCAC GCCTTTGGTC 
ATCACCACAG CTGTGACCTT TGGTAGCAAT GGCACCATCC TAGAGGAGGG CCTGGACCCT 
GACAATGGAA TTGAACTGGA GACCCTGGAG ACTGAGTTAG CCAGAGAAGA TGAAGAAGAA 
GATGGTCCCA CCATGGGCCC CCTCTTCCGG GCAGCAGAAC AGTCATGGAA TACTGAATTC 
CAGATCTTTC CTGGTGCTGG GGAGAAAGGA GAAAAAGGAG AGCCAGCCGT GGTTGAACAG 
GGACAGCAGT TTGAAGGACC CCCAGGAGCC CCAGGACCCC AAGGGGTGGT TGGCCCCTCA 
GGCCCTCCTG GGCCCCCAGG ATTCCCTGGA GACCGTGGTC TACCGGGCCC TGCTGGCCTC 
CCAGGCATCC CTGGCATTGA TGGGATCCGG GGACTCCCAG GCACTGTGAT CATGATACCG 
TTCCAGTTTG CAGGAGGCTC TCACAAAGGA CCCCCAGTAT CCTTCCAGCA GGCCCAGGCT 
CAGGCAGTCC TGCAACAAGC TCAGCTCTCT ATGAAAGGCC CTCCTGGCCC GGTGGGGCTC 
ACTGGACGCC CAGGTCCCGT GGGCCTCCCT GGGTATCCAG GTCTGAAAGG AGAGATGGGA 
GAAATGGGGC CTCAGGGTCC CCGAGGGCTT CAGGGACCTC ATGGGCCCCC TGGCCGAGAG 
GGAAAGATGG GCCGCCCTGG AGCAGATGGG GCTCGGGGCC TCCCAGGCAC CACGGGACCT 
AAGGGTGATC GGGGCTTTGA TGGCCTGCCA GGGCTGCCTG GTGAGAAGGG CCAAAGGGGT 
GACTTTGGTT ATGTGGGGAA ACCTGGTCCC CCAGGAGAAG ATGGGCGGAA GGGAGTAGAG 
GGACCTCCGG GGCCCACTGG CCAGGCGGGG GAGCCGGGCC CCCGAGGACT GATTGGCCCT 
AGAGGCTCCC CCGGACCCCT GGGACGCCCG GGTGTGACTG GAAGTGATGG TGCTCCAGGT 
GCCAAAGGAA ATGTGGGTCC TCCAGGAGAA CCAGGCCCCC CAGGACAGCA GGGAAATCCT 
GGGTCCCAGG GAATTCCTGG CCCCCAGGGG CCCATCGGCA CTCCTGGGGA GAAGGGTCCC 
CCTGGAAACC CAGGAATTCC AGGCCTCCCA GGATCTGAAG GCCCTCCGGG TCACCCAGGT 
CATGAGGGCC CAACAGGAGA GAAAGGGGCC CAGGGTCCAA CAGGGTCAGC AGGCCCTCCA 
GGCTATCCGG GACCTCGGGG TGTGAAGGGT ACTTCTGGCA ACCGAGGCCT CCAGGGGGAG 
AAAGGAGAGA GGGGAGAGGA TGGTTTCCCA GGCTTCAAGG GTGATGCGGG GCTTAAAGGA 
GATCGGGGGC AACCCGGACC TCCAGGTCCT CGGGGAGAGG ATGGTCCTGA AGGGCTCAAG 
GGGCAGGAGG GGCTGGTTGG CGAGGAGGGT CCCCCAGGCT CGGCTGGGGA GAAGGGCAAG 
CTTGGCGTGC CGGGTCTTCC AGGTTATCCA GGACGTCCAG GCCCTAAGGG ATCTATTGGC 
TTTCCGGGAC CCCTGGGACC ATTAGGAGAA AAAGGGAAGC GGGGGAAGGC TGGGCAGCCA 
GGCCTGGAAG GAGAGCGGGG ACCACCAGGT GCCCGTGGAG AGAGAGGGCA ACCAGGTGCC 
ACAGGGCAGC CGGGCCCTAA GGGTGATGTG GGCCAGGATG GAGCCCCTGG GTTCCCTGGA 
GAAAAGGGCC TCCCAGGTCT ACAAGGTCCT CCTGGGTTCT CCGGACCAAA GGGTCCCCCT 
GGTCCCCAGG GGAAAGATGG GCTACCTGGA CACCCTGGAC AGAGAGGAGA ATTGGGTTTC 
CAAGGTCAGA CAGGCCCACC TGGACCAGCT GGAGTTGTGG GTCCTCAGGG AAAGACAGGA 
GAAGCAGGGC CTCTGGGTGA GAGGGGGCCT CCAGGGCCCC CTGGACCTCC AGGTGAACAA 
GGTCTTCCGG GCCTGGAAGG CAGAGAGGGG GCCAAGGGGG ACGTGGGACC ATTGGGACCC 
CTTGGGAAGG AAGGGCCACC TGGACCCAGA GGTTTCCCTG GTCCTCATGG AGCCCCCGGG 
GAACCAGGAC CAACTGGTTT AAAGGGTGAC AAGGGCCCCC CAGGTCCTGT TGGTGCCAAC 
GGGCCCCCCG GGGGGGGGGG CCCTGTAGGC CCAGCAGGGG GCATTGGGCT TCCTGGTCAA 
AGTGGAGGCC AAGGCCCCGT GGGCCCCACA GGCGAGAAGG GTTCCCCGGG TGAACGTGGC 
GCCCCTGGCC CCACTGGTAA AGATGGCATC CCCGGGCCCC TGGGCCTTCC TGGACCCCCT 
GGAGCTGCGG GGGCTTCTGG GGGGGAAGGG GGCAAGGGGA GAGGGGGGAT AGAAGTGGGT 
GCCCCCGGTC ACAAAGGCAG CAAAGGAGAC AAGGGAGATG CGGGCCCGCC TGGACCAACA 
GGGATACGGG GTCTGGTGGG ACACCCAGGC TCTCCAGGAG CAGATGGGGC TCAGGGACGC 
CGGGGACCCC CAGGCCTCTT TGGGCAGAAA GGAGATGATG GAGTGAGGGG CTTCCCTGGC 
ACGATTGGTC CTCCTGGCCT ACAGGGAATG CCAGGCCCTG CTGGAGAAAA GGGAGAAGTT 
GGAGATGTAG GGTCCATGGG TCCCCATGGA GCTCCAGGCC CTCGGGGCCC CCAGGGCCCC 
AGCGGATCAG AGGGTCCTCC AGGGTTGCCT GGGGGAGTTG GTCAGCCCGG CGCTGTGGGT 
GAGAAGGGTG AGCCAGGAGA GCCTGGAGAC CCAGGACCCC CAGGGACCCC AGGCGCCCCG 
GGCCCCAGGG GAGAAGTTGG TGAAAAGGGG GATACAGGCC CATCTGGGGC TGCTGGACCC 
CCAGGCAAGA AAGGCCCCCC TGGAGAGGAT GGAGCAAAGG GGAATGTGGG CCCCGCAGGC 
CTCCCAGGAG ACCTAGGCCC CCCCGGAGAC CCTGGAGTTT CGGGTCAAGG TGGCCCCCCA 
GGGGAGAAGG GAAACCCTGG AGATGTGGGG GGACCGGGTC CACCTGGCGC TTCTGGGGAG 
CCTGGTGCCC CTGGGCCTCC TGGTAAAAGG GGTCCTTCTG GTCGCATGGG TCGAGAAGGC 
AGAGAAGGAG AGAAAGGTGC CAAGGGGGAA CCAGGTCCCG ATGGACCCCC AGGGAGGACA 
GGCCCAGTGG GGGCTGTAGG GCCCCCTGGA CGTGTTGGAC CTGAGGGTCT TCGTGGGATC 
CCTGGACCTG TGGGTGAACC AGGCCTCCTG GGACCTCCTG GACTGCTAGG CCCTCCTGGT 
CCCCTGGGGC CCTCTGGCCT ACCAGGGCTG AAGGGAGATG CTGGCCCCAA GGGAGAAAAG 
GGCCACATTG GATTAACTGG TCTCATCGGC CCCCCTGGGG AGGCTGGTGA GAAAGGGGAT 
CAGGGGTTGC CAGGAGTCCA AGGCCCCCCT GGCCTCCAGG GAGAACCGGG TTCCCCTGGC 
CCCATTGGCT CTCTGGGCCA CCCTGGGCCC CAAGGCGTGG TGGGTCCTCC AGGACAGAAA 
GGCTCAAAGG GGTCCCCAGG AACCCAAGGT CCTCGTGGAG ACACCGGCCC CCCAGGTCCT 
CCTGGTCCCC CGGGTCCCTC TGCGGAGCTG CATGGGCTGC GCCGGCGCCG ACGTTCCCTG 
CCAGGCTTGG GGACCCCGGA GGGCGGCCTG GAGGAGGTGC TGGCCTCGAT GGCGTCGCTG 
AGCTTCGAGT TAGAGCAGCT GCGGCGCCCT CCCGGCACGG CTGAGCGCCC GGGCCTCGTG 
TGCAAAGAGT TGCACCGCAG CCATCCACAT CCGCCGGATG GGGAATACTG GATTGATCCC 
AATCAAGGCT GCGCGCGGGA TGCACTCAGG GTTTTCTGTA ACTTCACGGC AGGAGGAGAA 
ACCTGCCTCT ACCCAGACAA GAAGTTTGAG ACAGTGAAAC TGGCCTCTTG GTCCAAAGAA 
AAGCCAGGTG GCTGGTATAG CACATTCCGT CGAGGGAAAA AGTTCTCCTA CGTGGATGCC 
GATGGGGCCC CGGTGAGTGT GGTACAGCTG ACCTTCCTGA AGCTGCTGAG TGCCACGGCC 
CGCCAGAGCT TCACGTTCAC CTGCCAGAAC TCGGCCGCCT GGCTGGACGA AGCTGCAGGT 
GACCACAGCC GCTCTGCCCG TTTCCTCGGG GCCAATGGGG AGGAGCTGTC CTTCAACCAG 
ACAGCAGCCG CCACCATCAC GGTCCCCTAC GATGGCTGCA GGCTCCGGAA GGGACAGTCG 
AAGACCCTCT TTGAGTTCAA CTCCTCCCGA GTGGGATTTC TGCCCTTGTT GGATGTGGCA 
GCTGCTGACT TTGGCCAGAC GAACCAGAAG TTTGGGTTTG AACTGGGCCC TGTCTGCTTT 
AGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_027969601.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027825402.1 Ovis aries collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.

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CloneID	OSm80267
Clone ID Related Accession (Same CDS sequence)	XM_027969601.1
Accession Version	XM_027969601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	collagen alpha-3(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGCA GATCCTGTGG ATGTGCTGAA GGCGCTGGGC 
ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGCCCCGGCC TCTGCCCCCA GAGGGCCCCA 
GAGGGTGACC GAGCATTCCG GGTGGGCAAG ACCAGCACAC TTGGTGTCCC CACGCAAGAG 
CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG 
CCAACCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GTGGCGTCCG GCAGCTGGGC 
TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG 
GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG 
ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCGTGTTGG CTCAGGGACC TCGATTCATC 
AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA 
GATGTCCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCCG CCTTCCAGGC ATGTGAGCGG 
TACCTTCCTG GCTGTGATGA CCTGGATCCT ACAGTCACCA GGGCCCCTCA GGGGGAGCCA 
GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAGAAAGG GCGAGGTCGT 
AAGGGGAAGG GCAGGAAGAA GAAGCTCAAG GAAGCTTTGG CTGCGAGTCT GCCTCCTGGG 
CCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCACCTCTG 
TCTCCGACAT CCACGCCTTT GGTCATCACC ACAGCTGTGA CCTTTGGTAG CAATGGCACC 
ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAATTGAAC TGGAGACCCT GGAGACTGAG 
TTAGCCAGAG AAGATGAAGA AGAAGATGGT CCCACCATGG GCCCCCTCTT CCGGGCAGCA 
GAACAGTCAT GGAATACTGA ATTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA 
GGAGAGCCAG CCGTGGTTGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCAGGA 
CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGGCCCC CAGGATTCCC TGGAGACCGT 
GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TTGATGGGAT CCGGGGACTC 
CCAGGCACTG TGATCATGAT ACCGTTCCAG TTTGCAGGAG GCTCTCACAA AGGACCCCCA 
GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT CTCTATGAAA 
GGCCCTCCTG GCCCGGTGGG GCTCACTGGA CGCCCAGGTC CCGTGGGCCT CCCTGGGTAT 
CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCTCAGG GTCCCCGAGG GCTTCAGGGA 
CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCC CTGGAGCAGA TGGGGCTCGG 
GGCCTCCCAG GCACCACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG 
CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA 
GAAGATGGGC GGAAGGGAGT AGAGGGACCT CCGGGGCCCA CTGGCCAGGC GGGGGAGCCG 
GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG 
ACTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC 
CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CTGGCCCCCA GGGGCCCATC 
GGCACTCCTG GGGAGAAGGG TCCCCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT 
GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT 
CCAACAGGGT CAGCAGGCCC TCCGGGCTAT CCGGGACCTC GGGGTGTGAA GGGTACTTCT 
GGCAACCGAG GCCTCCAGGG GGAGAAAGGA GAGAGGGGAG AGGATGGTTT CCCAGGCTTC 
AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA 
GAGGATGGTC CTGAAGGGCT CAAGGGGCAG GAGGGGCTGG TTGGCGAGGA GGGTCCCCCA 
GGCTCGGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TTCCAGGTTA TCCAGGACGT 
CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCCCTGG GACCATTAGG AGAAAAAGGG 
AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT 
GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CTAAGGGTGA TGTGGGCCAG 
GATGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTACAAGG TCCTCCTGGG 
TTCTCCGGAC CAAAGGGTCC CCCTGGTCCC CAGGGGAAAG ATGGGCTACC TGGACACCCT 
GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGAGTT 
GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCTCCAGGG 
CCCCCTGGAC CTCCAGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG 
GGGGACGTGG GACCATTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC 
CCTGGTCCTC ATGGAGCCCC CGGGGAACCA GGACCAACTG GTTTAAAGGG TGACAAGGGC 
CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA 
GGGGGCATTG GGCTTCCTGG TCAAAGTGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG 
AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCCACTG GTAAAGATGG CATCCCCGGG 
CCCCTGGGCC TTCCTGGACC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG 
GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGAG ACAAGGGAGA TGCGGGCCCG 
CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCAG GTTCTCCAGG AGCAGATGGG 
GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGG 
GGCTTCCCTG GCACGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA 
AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCATG GAGCTCCAGG CCCTCGGGGC 
CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCC 
GGCGCTGTGG GTGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCAGGGACC 
CCAGGCGCCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG 
GCTGCTGGAC CCCCAGGCAA GAAAGGCCCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG 
GGCCCCGCAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA 
GGTGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC 
GCTTCTGGGG AGCCTGGTGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG 
GGTCGAGAAG GCAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC CGATGGACCC 
CCAGGGAGGA CAGGCCCAGT GGGGGCTGTA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT 
CTTCGTGGGA TCCCTGGACC TGTGGGTGAA CCAGGCCTCC TGGGACCTCC TGGACTGCTA 
GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC 
AAGGGAGAAA AGGGCCACAT TGGATTAACT GGTCTCATCG GCCCCCCTGG GGAGGCTGGT 
GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCCCCC CTGGCCTCCA GGGAGAACCG 
GGTTCCCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT 
CCAGGACAGA AAGGCTCAAA GGGGTCCCCA GGAACCCAAG GTCCTCGTGG AGACACCGGC 
CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCATGGGCT GCGCCGGCGC 
CGACGTTCCC TGCCAGGCTT GGGGACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC 
CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCAC ATCTGCCGGA TGGGGAATAC 
TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCACTCA GGGTTTTCTG TAACTTCACG 
GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACAGTGAA ACTGGCCTCT 
TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC 
TACGTGGATG CCGATGGGGC CCCGGTGAGT GTGGTACAGC TGACCTTCCT GAAGCTGCTG 
AGTGCCACGG CCCGCCAGAG CTTCACGTTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC 
GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGCTG 
TCCTTCAACC AGACAGCAGC CGCCACCATC ACGGTCCCCT ACGATGGCTG CAGGCTCCGG 
AAGGGACAGT CGAAGACCCT CTTTGAGTTC AACTCCTCCC GAGTGGGATT TCTGCCCTTG 
TTGGATGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC 
CCTGTCTGCT TTAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027825402.1
CDS	161..5401
Translation	MGSRRGPGQPRAGLCLLLASLQLLPRTQAADPVDVLKALGMRGGQAGIPEGPGLCPQRAPEGDRAFRVGKTSTLGVPTQELFQDGHFPENFSILITLRGQPTNRSVLLSIYDEGGVRQLGLALGPALDLLGDSFSPLPQQVNLTDGRWHRVAVSVDGGMVTLVADCEPQLPVLAQGPRFISTAGLTVLGTQDLGEETFEGDVQELVISPDPQAAFQACERYLPGCDDLDPTVTRAPQGEPETPPPRRKGKGKGKKKGRGRKGKGRKKKLKEALAASLPPGPLDNQTSTDIPKTETPEPPLSPTSTPLVITTAVTFGSNGTILEEGLDPDNGIELETLETELAREDEEEDGPTMGPLFRAAEQSWNTEFQIFPGAGEKGEKGEPAVVEQGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGIRGLPGTVIMIPFQFAGGSHKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGEMGEMGPQGPRGLQGPHGPPGREGKMGRPGADGARGLPGTTGPKGDRGFDGLPGLPGEKGQRGDFGYVGKPGPPGEDGRKGVEGPPGPTGQAGEPGPRGLIGPRGSPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNPGSQGIPGPQGPIGTPGEKGPPGNPGIPGLPGSEGPPGHPGHEGPTGEKGAQGPTGSAGPPGYPGPRGVKGTSGNRGLQGEKGERGEDGFPGFKGDAGLKGDRGQPGPPGPRGEDGPEGLKGQEGLVGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGLEGERGPPGARGERGQPGATGQPGPKGDVGQDGAPGFPGEKGLPGLQGPPGFSGPKGPPGPQGKDGLPGHPGQRGELGFQGQTGPPGPAGVVGPQGKTGEAGPLGERGPPGPPGPPGEQGLPGLEGREGAKGDVGPLGPLGKEGPPGPRGFPGPHGAPGEPGPTGLKGDKGPPGPVGANGSPGERGPVGPAGGIGLPGQSGGQGPVGPTGEKGSPGERGAPGPTGKDGIPGPLGLPGPPGAAGPSGEEGDKGEVGAPGHKGSKGDKGDAGPPGPTGIRGLVGHPGSPGADGAQGRRGPPGLFGQKGDDGVRGFPGTIGPPGLQGMPGPAGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGPPGLPGGVGQPGAVGEKGEPGEPGDPGPPGTPGAPGPRGEVGEKGDTGPSGAAGPPGKKGPPGEDGAKGNVGPAGLPGDLGPPGDPGVSGQGGPPGEKGNPGDVGGPGPPGASGEPGAPGPPGKRGPSGRMGREGREGEKGAKGEPGPDGPPGRTGPVGAVGPPGRVGPEGLRGIPGPVGEPGLLGPPGLLGPPGPLGPSGLPGLKGDAGPKGEKGHIGLTGLIGPPGEAGEKGDQGLPGVQGPPGLQGEPGSPGPIGSLGHPGPQGVVGPPGQKGSKGSPGTQGPRGDTGPPGPPGPPGPSAELHGLRRRRRSLPGLGTPEGGLEEVLASMASLSFELEQLRRPPGTAERPGLVCKELHRSHPHLPDGEYWIDPNQGCARDALRVFCNFTAGGETCLYPDKKFETVKLASWSKEKPGGWYSTFRRGKKFSYVDADGAPVSVVQLTFLKLLSATARQSFTFTCQNSAAWLDEAAGDHSRSARFLGANGEELSFNQTAAATITVPYDGCRLRKGQSKTLFEFNSSRVGFLPLLDVAAADFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_027969601.1
Organism	Ovis aries(sheep)
Definition	Ovis aries collagen type V alpha 3 chain (COL5A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027969601.1

ORF Insert Sequence:

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGCA GATCCTGTGG ATGTGCTGAA GGCGCTGGGC 
ATGCGGGGGG GCCAGGCTGG GATCCCTGAG GGCCCCGGCC TCTGCCCCCA GAGGGCCCCA 
GAGGGTGACC GAGCATTCCG GGTGGGCAAG ACCAGCACAC TTGGTGTCCC CACGCAAGAG 
CTCTTCCAAG ATGGGCACTT TCCTGAGAAC TTCTCCATCC TGATCACCCT GCGGGGCCAG 
CCAACCAACC GTTCTGTCCT TCTGTCCATC TATGATGAGG GTGGCGTCCG GCAGCTGGGC 
TTGGCCTTGG GGCCAGCTCT GGACCTCCTA GGTGACTCCT TCAGCCCCCT CCCCCAGCAG 
GTCAACCTCA CGGATGGCAG GTGGCACCGT GTGGCAGTCA GTGTGGACGG TGGGATGGTG 
ACCCTGGTGG CTGATTGTGA ACCTCAGCTC CCCGTGTTGG CTCAGGGACC TCGATTCATC 
AGTACAGCGG GACTCACTGT GCTGGGGACC CAGGACCTTG GCGAGGAGAC TTTTGAGGGA 
GATGTCCAGG AGCTGGTGAT AAGCCCAGAT CCCCAGGCCG CCTTCCAGGC ATGTGAGCGG 
TACCTTCCTG GCTGTGATGA CCTGGATCCT ACAGTCACCA GGGCCCCTCA GGGGGAGCCA 
GAAACCCCTC CCCCTAGGCG GAAGGGGAAA GGAAAAGGGA AGAAGAAAGG GCGAGGTCGT 
AAGGGGAAGG GCAGGAAGAA GAAGCTCAAG GAAGCTTTGG CTGCGAGTCT GCCTCCTGGG 
CCCCTGGACA ACCAGACCTC CACTGACATC CCCAAGACAG AGACACCAGA ACCACCTCTG 
TCTCCGACAT CCACGCCTTT GGTCATCACC ACAGCTGTGA CCTTTGGTAG CAATGGCACC 
ATCCTAGAGG AGGGCCTGGA CCCTGACAAT GGAATTGAAC TGGAGACCCT GGAGACTGAG 
TTAGCCAGAG AAGATGAAGA AGAAGATGGT CCCACCATGG GCCCCCTCTT CCGGGCAGCA 
GAACAGTCAT GGAATACTGA ATTCCAGATC TTTCCTGGTG CTGGGGAGAA AGGAGAAAAA 
GGAGAGCCAG CCGTGGTTGA ACAGGGACAG CAGTTTGAAG GACCCCCAGG AGCCCCAGGA 
CCCCAAGGGG TGGTTGGCCC CTCAGGCCCT CCTGGGCCCC CAGGATTCCC TGGAGACCGT 
GGTCTACCGG GCCCTGCTGG CCTCCCAGGC ATCCCTGGCA TTGATGGGAT CCGGGGACTC 
CCAGGCACTG TGATCATGAT ACCGTTCCAG TTTGCAGGAG GCTCTCACAA AGGACCCCCA 
GTATCCTTCC AGCAGGCCCA GGCTCAGGCA GTCCTGCAAC AAGCTCAGCT CTCTATGAAA 
GGCCCTCCTG GCCCGGTGGG GCTCACTGGA CGCCCAGGTC CCGTGGGCCT CCCTGGGTAT 
CCAGGTCTGA AAGGAGAGAT GGGAGAAATG GGGCCTCAGG GTCCCCGAGG GCTTCAGGGA 
CCTCATGGGC CCCCTGGCCG AGAGGGAAAG ATGGGCCGCC CTGGAGCAGA TGGGGCTCGG 
GGCCTCCCAG GCACCACGGG ACCTAAGGGT GATCGGGGCT TTGATGGCCT GCCAGGGCTG 
CCTGGTGAGA AGGGCCAAAG GGGTGACTTT GGTTATGTGG GGAAACCTGG TCCCCCAGGA 
GAAGATGGGC GGAAGGGAGT AGAGGGACCT CCGGGGCCCA CTGGCCAGGC GGGGGAGCCG 
GGCCCCCGAG GACTGATTGG CCCTAGAGGC TCCCCCGGAC CCCTGGGACG CCCGGGTGTG 
ACTGGAAGTG ATGGTGCTCC AGGTGCCAAA GGAAATGTGG GTCCTCCAGG AGAACCAGGC 
CCCCCAGGAC AGCAGGGAAA TCCTGGGTCC CAGGGAATTC CTGGCCCCCA GGGGCCCATC 
GGCACTCCTG GGGAGAAGGG TCCCCCTGGA AACCCAGGAA TTCCAGGCCT CCCAGGATCT 
GAAGGCCCTC CGGGTCACCC AGGTCATGAG GGCCCAACAG GAGAGAAAGG GGCCCAGGGT 
CCAACAGGGT CAGCAGGCCC TCCGGGCTAT CCGGGACCTC GGGGTGTGAA GGGTACTTCT 
GGCAACCGAG GCCTCCAGGG GGAGAAAGGA GAGAGGGGAG AGGATGGTTT CCCAGGCTTC 
AAGGGTGATG CGGGGCTTAA AGGAGATCGG GGGCAACCCG GACCTCCAGG TCCTCGGGGA 
GAGGATGGTC CTGAAGGGCT CAAGGGGCAG GAGGGGCTGG TTGGCGAGGA GGGTCCCCCA 
GGCTCGGCTG GGGAGAAGGG CAAGCTTGGC GTGCCGGGTC TTCCAGGTTA TCCAGGACGT 
CCAGGCCCTA AGGGATCTAT TGGCTTTCCG GGACCCCTGG GACCATTAGG AGAAAAAGGG 
AAGCGGGGGA AGGCTGGGCA GCCAGGCCTG GAAGGAGAGC GGGGACCACC AGGTGCCCGT 
GGAGAGAGAG GGCAACCAGG TGCCACAGGG CAGCCGGGCC CTAAGGGTGA TGTGGGCCAG 
GATGGAGCCC CTGGGTTCCC TGGAGAAAAG GGCCTCCCAG GTCTACAAGG TCCTCCTGGG 
TTCTCCGGAC CAAAGGGTCC CCCTGGTCCC CAGGGGAAAG ATGGGCTACC TGGACACCCT 
GGACAGAGAG GAGAATTGGG TTTCCAAGGT CAGACAGGCC CACCTGGACC AGCTGGAGTT 
GTGGGTCCTC AGGGAAAGAC AGGAGAAGCA GGGCCTCTGG GTGAGAGGGG GCCTCCAGGG 
CCCCCTGGAC CTCCAGGTGA ACAAGGTCTT CCGGGCCTGG AAGGCAGAGA GGGGGCCAAG 
GGGGACGTGG GACCATTGGG ACCCCTTGGG AAGGAAGGGC CACCTGGACC CAGAGGTTTC 
CCTGGTCCTC ATGGAGCCCC CGGGGAACCA GGACCAACTG GTTTAAAGGG TGACAAGGGC 
CCCCCAGGTC CTGTTGGTGC CAACGGATCC CCTGGGGAGC GTGGTCCTGT AGGCCCAGCA 
GGGGGCATTG GGCTTCCTGG TCAAAGTGGA GGCCAAGGCC CCGTGGGCCC CACAGGCGAG 
AAGGGTTCCC CGGGTGAACG TGGCGCCCCT GGCCCCACTG GTAAAGATGG CATCCCCGGG 
CCCCTGGGCC TTCCTGGACC CCCTGGAGCT GCGGGGCCTT CTGGCGAGGA AGGGGACAAG 
GGAGAAGTGG GTGCCCCCGG TCACAAAGGC AGCAAAGGAG ACAAGGGAGA TGCGGGCCCG 
CCTGGACCAA CAGGGATACG GGGTCTGGTG GGACACCCAG GTTCTCCAGG AGCAGATGGG 
GCTCAGGGAC GCCGGGGACC CCCAGGCCTC TTTGGGCAGA AAGGAGATGA TGGAGTGAGG 
GGCTTCCCTG GCACGATTGG TCCTCCTGGC CTACAGGGAA TGCCAGGCCC TGCTGGAGAA 
AAGGGAGAAG TTGGAGATGT AGGGTCCATG GGTCCCCATG GAGCTCCAGG CCCTCGGGGC 
CCCCAGGGCC CCAGCGGATC AGAGGGTCCT CCAGGGTTGC CTGGGGGAGT TGGTCAGCCC 
GGCGCTGTGG GTGAGAAGGG TGAGCCAGGA GAGCCTGGAG ACCCAGGACC CCCAGGGACC 
CCAGGCGCCC CGGGCCCCAG GGGAGAAGTT GGTGAAAAGG GGGATACAGG CCCATCTGGG 
GCTGCTGGAC CCCCAGGCAA GAAAGGCCCC CCTGGAGAGG ATGGAGCAAA GGGGAATGTG 
GGCCCCGCAG GCCTCCCAGG AGACCTAGGC CCCCCCGGAG ACCCTGGAGT TTCGGGTCAA 
GGTGGCCCCC CAGGGGAGAA GGGAAACCCT GGAGATGTGG GGGGACCGGG TCCACCTGGC 
GCTTCTGGGG AGCCTGGTGC CCCTGGGCCT CCTGGTAAAA GGGGTCCTTC TGGTCGCATG 
GGTCGAGAAG GCAGAGAAGG AGAGAAAGGT GCCAAGGGGG AACCAGGTCC CGATGGACCC 
CCAGGGAGGA CAGGCCCAGT GGGGGCTGTA GGGCCCCCTG GACGTGTTGG ACCTGAGGGT 
CTTCGTGGGA TCCCTGGACC TGTGGGTGAA CCAGGCCTCC TGGGACCTCC TGGACTGCTA 
GGCCCTCCTG GTCCCCTGGG GCCCTCTGGC CTACCAGGGC TGAAGGGAGA TGCTGGCCCC 
AAGGGAGAAA AGGGCCACAT TGGATTAACT GGTCTCATCG GCCCCCCTGG GGAGGCTGGT 
GAGAAAGGGG ATCAGGGGTT GCCAGGAGTG CAAGGCCCCC CTGGCCTCCA GGGAGAACCG 
GGTTCCCCTG GCCCCATTGG CTCTCTGGGC CACCCTGGGC CCCAAGGCGT GGTGGGTCCT 
CCAGGACAGA AAGGCTCAAA GGGGTCCCCA GGAACCCAAG GTCCTCGTGG AGACACCGGC 
CCCCCAGGTC CTCCTGGTCC CCCGGGTCCC TCTGCGGAGC TGCATGGGCT GCGCCGGCGC 
CGACGTTCCC TGCCAGGCTT GGGGACCCCG GAGGGCGGCC TGGAGGAGGT GCTGGCCTCG 
ATGGCGTCGC TGAGCTTCGA GTTAGAGCAG CTGCGGCGCC CTCCCGGCAC GGCTGAGCGC 
CCGGGCCTCG TGTGCAAAGA GTTGCACCGC AGCCATCCAC ATCTGCCGGA TGGGGAATAC 
TGGATTGATC CCAATCAAGG CTGCGCGCGG GATGCACTCA GGGTTTTCTG TAACTTCACG 
GCAGGAGGAG AAACCTGCCT CTACCCAGAC AAGAAGTTTG AGACAGTGAA ACTGGCCTCT 
TGGTCCAAAG AAAAGCCAGG TGGCTGGTAT AGCACATTCC GTCGAGGGAA AAAGTTCTCC 
TACGTGGATG CCGATGGGGC CCCGGTGAGT GTGGTACAGC TGACCTTCCT GAAGCTGCTG 
AGTGCCACGG CCCGCCAGAG CTTCACGTTC ACCTGCCAGA ACTCGGCCGC CTGGCTGGAC 
GAAGCTGCAG GTGACCACAG CCGCTCTGCC CGTTTCCTCG GGGCCAATGG GGAGGAGCTG 
TCCTTCAACC AGACAGCAGC CGCCACCATC ACGGTCCCCT ACGATGGCTG CAGGCTCCGG 
AAGGGACAGT CGAAGACCCT CTTTGAGTTC AACTCCTCCC GAGTGGGATT TCTGCCCTTG 
TTGGATGTGG CAGCTGCTGA CTTTGGCCAG ACGAACCAGA AGTTTGGGTT TGAACTGGGC 
CCTGTCTGCT TTAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A3 ( XM_027969602.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027825403.1 Ovis aries collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.

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CloneID	OSm80268
Clone ID Related Accession (Same CDS sequence)	XM_027969602.1
Accession Version	XM_027969602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	collagen alpha-3(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC GCTGGGCATG 
CGGGGGGGCC AGGCTGGGAT CCCTGAGGGC CCCGGCCTCT GCCCCCAGAG GGCCCCAGAG 
GGTGACCGAG CATTCCGGGT GGGCAAGACC AGCACACTTG GTGTCCCCAC GCAAGAGCTC 
TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCA 
ACCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGTG GCGTCCGGCA GCTGGGCTTG 
GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC 
AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC 
CTGGTGGCTG ATTGTGAACC TCAGCTCCCC GTGTTGGCTC AGGGACCTCG ATTCATCAGT 
ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT 
GTCCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCCGCCT TCCAGGCATG TGAGCGGTAC 
CTTCCTGGCT GTGATGACCT GGATCCTACA GTCACCAGGG CCCCTCAGGG GGAGCCAGAA 
ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AGAAAGGGCG AGGTCGTAAG 
GGGAAGGGCA GGAAGAAGAA GCTCAAGGAA GCTTTGGCTG CGAGTCTGCC TCCTGGGCCC 
CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC ACCTCTGTCT 
CCGACATCCA CGCCTTTGGT CATCACCACA GCTGTGACCT TTGGTAGCAA TGGCACCATC 
CTAGAGGAGG GCCTGGACCC TGACAATGGA ATTGAACTGG AGACCCTGGA GACTGAGTTA 
GCCAGAGAAG ATGAAGAAGA AGATGGTCCC ACCATGGGCC CCCTCTTCCG GGCAGCAGAA 
CAGTCATGGA ATACTGAATT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA 
GAGCCAGCCG TGGTTGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCAGGACCC 
CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGGCCCCCAG GATTCCCTGG AGACCGTGGT 
CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATTG ATGGGATCCG GGGACTCCCA 
GGCACTGTGA TCATGATACC GTTCCAGTTT GCAGGAGGCT CTCACAAAGG ACCCCCAGTA 
TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTCTC TATGAAAGGC 
CCTCCTGGCC CGGTGGGGCT CACTGGACGC CCAGGTCCCG TGGGCCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCTCAGGGTC CCCGAGGGCT TCAGGGACCT 
CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCCCTG GAGCAGATGG GGCTCGGGGC 
CTCCCAGGCA CCACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT 
GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAA 
GATGGGCGGA AGGGAGTAGA GGGACCTCCG GGGCCCACTG GCCAGGCGGG GGAGCCGGGC 
CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGACT 
GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC 
CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCTG GCCCCCAGGG GCCCATCGGC 
ACTCCTGGGG AGAAGGGTCC CCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA 
GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA 
ACAGGGTCAG CAGGCCCTCC GGGCTATCCG GGACCTCGGG GTGTGAAGGG TACTTCTGGC 
AACCGAGGCC TCCAGGGGGA GAAAGGAGAG AGGGGAGAGG ATGGTTTCCC AGGCTTCAAG 
GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG 
GATGGTCCTG AAGGGCTCAA GGGGCAGGAG GGGCTGGTTG GCGAGGAGGG TCCCCCAGGC 
TCGGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTTC CAGGTTATCC AGGACGTCCA 
GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCCCTGGGAC CATTAGGAGA AAAAGGGAAG 
CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA 
GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCTA AGGGTGATGT GGGCCAGGAT 
GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TACAAGGTCC TCCTGGGTTC 
TCCGGACCAA AGGGTCCCCC TGGTCCCCAG GGGAAAGATG GGCTACCTGG ACACCCTGGA 
CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGAGTTGTG 
GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC TCCAGGGCCC 
CCTGGACCTC CAGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GACGTGGGAC CATTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT 
GGTCCTCATG GAGCCCCCGG GGAACCAGGA CCAACTGGTT TAAAGGGTGA CAAGGGCCCC 
CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG 
GGCATTGGGC TTCCTGGTCA AAGTGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG 
GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCCACTGGTA AAGATGGCAT CCCCGGGCCC 
CTGGGCCTTC CTGGACCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA 
GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGAGACA AGGGAGATGC GGGCCCGCCT 
GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCAGGTT CTCCAGGAGC AGATGGGGCT 
CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGGGGC 
TTCCCTGGCA CGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG 
GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCATGGAG CTCCAGGCCC TCGGGGCCCC 
CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCCGGC 
GCTGTGGGTG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC AGGGACCCCA 
GGCGCCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT 
GCTGGACCCC CAGGCAAGAA AGGCCCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC 
CCCGCAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGGT 
GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT 
TCTGGGGAGC CTGGTGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT 
CGAGAAGGCA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCCGA TGGACCCCCA 
GGGAGGACAG GCCCAGTGGG GGCTGTAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT 
CGTGGGATCC CTGGACCTGT GGGTGAACCA GGCCTCCTGG GACCTCCTGG ACTGCTAGGC 
CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG 
GGAGAAAAGG GCCACATTGG ATTAACTGGT CTCATCGGCC CCCCTGGGGA GGCTGGTGAG 
AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCCCCCCTG GCCTCCAGGG AGAACCGGGT 
TCCCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA 
GGACAGAAAG GCTCAAAGGG GTCCCCAGGA ACCCAAGGTC CTCGTGGAGA CACCGGCCCC 
CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ATGGGCTGCG CCGGCGCCGA 
CGTTCCCTGC CAGGCTTGGG GACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG 
GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG 
GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCACATC TGCCGGATGG GGAATACTGG 
ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCACTCAGGG TTTTCTGTAA CTTCACGGCA 
GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CAGTGAAACT GGCCTCTTGG 
TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC 
GTGGATGCCG ATGGGGCCCC GGTGAGTGTG GTACAGCTGA CCTTCCTGAA GCTGCTGAGT 
GCCACGGCCC GCCAGAGCTT CACGTTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA 
GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGCTGTCC 
TTCAACCAGA CAGCAGCCGC CACCATCACG GTCCCCTACG ATGGCTGCAG GCTCCGGAAG 
GGACAGTCGA AGACCCTCTT TGAGTTCAAC TCCTCCCGAG TGGGATTTCT GCCCTTGTTG 
GATGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT 
GTCTGCTTTA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027825403.1
CDS	161..5398
Translation	MGSRRGPGQPRAGLCLLLASLQLLPRTQADPVDVLKALGMRGGQAGIPEGPGLCPQRAPEGDRAFRVGKTSTLGVPTQELFQDGHFPENFSILITLRGQPTNRSVLLSIYDEGGVRQLGLALGPALDLLGDSFSPLPQQVNLTDGRWHRVAVSVDGGMVTLVADCEPQLPVLAQGPRFISTAGLTVLGTQDLGEETFEGDVQELVISPDPQAAFQACERYLPGCDDLDPTVTRAPQGEPETPPPRRKGKGKGKKKGRGRKGKGRKKKLKEALAASLPPGPLDNQTSTDIPKTETPEPPLSPTSTPLVITTAVTFGSNGTILEEGLDPDNGIELETLETELAREDEEEDGPTMGPLFRAAEQSWNTEFQIFPGAGEKGEKGEPAVVEQGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGIRGLPGTVIMIPFQFAGGSHKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGEMGEMGPQGPRGLQGPHGPPGREGKMGRPGADGARGLPGTTGPKGDRGFDGLPGLPGEKGQRGDFGYVGKPGPPGEDGRKGVEGPPGPTGQAGEPGPRGLIGPRGSPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNPGSQGIPGPQGPIGTPGEKGPPGNPGIPGLPGSEGPPGHPGHEGPTGEKGAQGPTGSAGPPGYPGPRGVKGTSGNRGLQGEKGERGEDGFPGFKGDAGLKGDRGQPGPPGPRGEDGPEGLKGQEGLVGEEGPPGSAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGLEGERGPPGARGERGQPGATGQPGPKGDVGQDGAPGFPGEKGLPGLQGPPGFSGPKGPPGPQGKDGLPGHPGQRGELGFQGQTGPPGPAGVVGPQGKTGEAGPLGERGPPGPPGPPGEQGLPGLEGREGAKGDVGPLGPLGKEGPPGPRGFPGPHGAPGEPGPTGLKGDKGPPGPVGANGSPGERGPVGPAGGIGLPGQSGGQGPVGPTGEKGSPGERGAPGPTGKDGIPGPLGLPGPPGAAGPSGEEGDKGEVGAPGHKGSKGDKGDAGPPGPTGIRGLVGHPGSPGADGAQGRRGPPGLFGQKGDDGVRGFPGTIGPPGLQGMPGPAGEKGEVGDVGSMGPHGAPGPRGPQGPSGSEGPPGLPGGVGQPGAVGEKGEPGEPGDPGPPGTPGAPGPRGEVGEKGDTGPSGAAGPPGKKGPPGEDGAKGNVGPAGLPGDLGPPGDPGVSGQGGPPGEKGNPGDVGGPGPPGASGEPGAPGPPGKRGPSGRMGREGREGEKGAKGEPGPDGPPGRTGPVGAVGPPGRVGPEGLRGIPGPVGEPGLLGPPGLLGPPGPLGPSGLPGLKGDAGPKGEKGHIGLTGLIGPPGEAGEKGDQGLPGVQGPPGLQGEPGSPGPIGSLGHPGPQGVVGPPGQKGSKGSPGTQGPRGDTGPPGPPGPPGPSAELHGLRRRRRSLPGLGTPEGGLEEVLASMASLSFELEQLRRPPGTAERPGLVCKELHRSHPHLPDGEYWIDPNQGCARDALRVFCNFTAGGETCLYPDKKFETVKLASWSKEKPGGWYSTFRRGKKFSYVDADGAPVSVVQLTFLKLLSATARQSFTFTCQNSAAWLDEAAGDHSRSARFLGANGEELSFNQTAAATITVPYDGCRLRKGQSKTLFEFNSSRVGFLPLLDVAAADFGQTNQKFGFELGPVCFSS

Target ORF information:

RefSeq Version	XM_027969602.1
Organism	Ovis aries(sheep)
Definition	Ovis aries collagen type V alpha 3 chain (COL5A3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027969602.1

ORF Insert Sequence:

ATGGGGAGTC GCCGGGGCCC GGGCCAGCCG CGGGCCGGCC TCTGCCTGCT CCTGGCCTCT 
CTGCAGCTTC TGCCCCGGAC GCAGGCCGAT CCTGTGGATG TGCTGAAGGC GCTGGGCATG 
CGGGGGGGCC AGGCTGGGAT CCCTGAGGGC CCCGGCCTCT GCCCCCAGAG GGCCCCAGAG 
GGTGACCGAG CATTCCGGGT GGGCAAGACC AGCACACTTG GTGTCCCCAC GCAAGAGCTC 
TTCCAAGATG GGCACTTTCC TGAGAACTTC TCCATCCTGA TCACCCTGCG GGGCCAGCCA 
ACCAACCGTT CTGTCCTTCT GTCCATCTAT GATGAGGGTG GCGTCCGGCA GCTGGGCTTG 
GCCTTGGGGC CAGCTCTGGA CCTCCTAGGT GACTCCTTCA GCCCCCTCCC CCAGCAGGTC 
AACCTCACGG ATGGCAGGTG GCACCGTGTG GCAGTCAGTG TGGACGGTGG GATGGTGACC 
CTGGTGGCTG ATTGTGAACC TCAGCTCCCC GTGTTGGCTC AGGGACCTCG ATTCATCAGT 
ACAGCGGGAC TCACTGTGCT GGGGACCCAG GACCTTGGCG AGGAGACTTT TGAGGGAGAT 
GTCCAGGAGC TGGTGATAAG CCCAGATCCC CAGGCCGCCT TCCAGGCATG TGAGCGGTAC 
CTTCCTGGCT GTGATGACCT GGATCCTACA GTCACCAGGG CCCCTCAGGG GGAGCCAGAA 
ACCCCTCCCC CTAGGCGGAA GGGGAAAGGA AAAGGGAAGA AGAAAGGGCG AGGTCGTAAG 
GGGAAGGGCA GGAAGAAGAA GCTCAAGGAA GCTTTGGCTG CGAGTCTGCC TCCTGGGCCC 
CTGGACAACC AGACCTCCAC TGACATCCCC AAGACAGAGA CACCAGAACC ACCTCTGTCT 
CCGACATCCA CGCCTTTGGT CATCACCACA GCTGTGACCT TTGGTAGCAA TGGCACCATC 
CTAGAGGAGG GCCTGGACCC TGACAATGGA ATTGAACTGG AGACCCTGGA GACTGAGTTA 
GCCAGAGAAG ATGAAGAAGA AGATGGTCCC ACCATGGGCC CCCTCTTCCG GGCAGCAGAA 
CAGTCATGGA ATACTGAATT CCAGATCTTT CCTGGTGCTG GGGAGAAAGG AGAAAAAGGA 
GAGCCAGCCG TGGTTGAACA GGGACAGCAG TTTGAAGGAC CCCCAGGAGC CCCAGGACCC 
CAAGGGGTGG TTGGCCCCTC AGGCCCTCCT GGGCCCCCAG GATTCCCTGG AGACCGTGGT 
CTACCGGGCC CTGCTGGCCT CCCAGGCATC CCTGGCATTG ATGGGATCCG GGGACTCCCA 
GGCACTGTGA TCATGATACC GTTCCAGTTT GCAGGAGGCT CTCACAAAGG ACCCCCAGTA 
TCCTTCCAGC AGGCCCAGGC TCAGGCAGTC CTGCAACAAG CTCAGCTCTC TATGAAAGGC 
CCTCCTGGCC CGGTGGGGCT CACTGGACGC CCAGGTCCCG TGGGCCTCCC TGGGTATCCA 
GGTCTGAAAG GAGAGATGGG AGAAATGGGG CCTCAGGGTC CCCGAGGGCT TCAGGGACCT 
CATGGGCCCC CTGGCCGAGA GGGAAAGATG GGCCGCCCTG GAGCAGATGG GGCTCGGGGC 
CTCCCAGGCA CCACGGGACC TAAGGGTGAT CGGGGCTTTG ATGGCCTGCC AGGGCTGCCT 
GGTGAGAAGG GCCAAAGGGG TGACTTTGGT TATGTGGGGA AACCTGGTCC CCCAGGAGAA 
GATGGGCGGA AGGGAGTAGA GGGACCTCCG GGGCCCACTG GCCAGGCGGG GGAGCCGGGC 
CCCCGAGGAC TGATTGGCCC TAGAGGCTCC CCCGGACCCC TGGGACGCCC GGGTGTGACT 
GGAAGTGATG GTGCTCCAGG TGCCAAAGGA AATGTGGGTC CTCCAGGAGA ACCAGGCCCC 
CCAGGACAGC AGGGAAATCC TGGGTCCCAG GGAATTCCTG GCCCCCAGGG GCCCATCGGC 
ACTCCTGGGG AGAAGGGTCC CCCTGGAAAC CCAGGAATTC CAGGCCTCCC AGGATCTGAA 
GGCCCTCCGG GTCACCCAGG TCATGAGGGC CCAACAGGAG AGAAAGGGGC CCAGGGTCCA 
ACAGGGTCAG CAGGCCCTCC GGGCTATCCG GGACCTCGGG GTGTGAAGGG TACTTCTGGC 
AACCGAGGCC TCCAGGGGGA GAAAGGAGAG AGGGGAGAGG ATGGTTTCCC AGGCTTCAAG 
GGTGATGCGG GGCTTAAAGG AGATCGGGGG CAACCCGGAC CTCCAGGTCC TCGGGGAGAG 
GATGGTCCTG AAGGGCTCAA GGGGCAGGAG GGGCTGGTTG GCGAGGAGGG TCCCCCAGGC 
TCGGCTGGGG AGAAGGGCAA GCTTGGCGTG CCGGGTCTTC CAGGTTATCC AGGACGTCCA 
GGCCCTAAGG GATCTATTGG CTTTCCGGGA CCCCTGGGAC CATTAGGAGA AAAAGGGAAG 
CGGGGGAAGG CTGGGCAGCC AGGCCTGGAA GGAGAGCGGG GACCACCAGG TGCCCGTGGA 
GAGAGAGGGC AACCAGGTGC CACAGGGCAG CCGGGCCCTA AGGGTGATGT GGGCCAGGAT 
GGAGCCCCTG GGTTCCCTGG AGAAAAGGGC CTCCCAGGTC TACAAGGTCC TCCTGGGTTC 
TCCGGACCAA AGGGTCCCCC TGGTCCCCAG GGGAAAGATG GGCTACCTGG ACACCCTGGA 
CAGAGAGGAG AATTGGGTTT CCAAGGTCAG ACAGGCCCAC CTGGACCAGC TGGAGTTGTG 
GGTCCTCAGG GAAAGACAGG AGAAGCAGGG CCTCTGGGTG AGAGGGGGCC TCCAGGGCCC 
CCTGGACCTC CAGGTGAACA AGGTCTTCCG GGCCTGGAAG GCAGAGAGGG GGCCAAGGGG 
GACGTGGGAC CATTGGGACC CCTTGGGAAG GAAGGGCCAC CTGGACCCAG AGGTTTCCCT 
GGTCCTCATG GAGCCCCCGG GGAACCAGGA CCAACTGGTT TAAAGGGTGA CAAGGGCCCC 
CCAGGTCCTG TTGGTGCCAA CGGATCCCCT GGGGAGCGTG GTCCTGTAGG CCCAGCAGGG 
GGCATTGGGC TTCCTGGTCA AAGTGGAGGC CAAGGCCCCG TGGGCCCCAC AGGCGAGAAG 
GGTTCCCCGG GTGAACGTGG CGCCCCTGGC CCCACTGGTA AAGATGGCAT CCCCGGGCCC 
CTGGGCCTTC CTGGACCCCC TGGAGCTGCG GGGCCTTCTG GCGAGGAAGG GGACAAGGGA 
GAAGTGGGTG CCCCCGGTCA CAAAGGCAGC AAAGGAGACA AGGGAGATGC GGGCCCGCCT 
GGACCAACAG GGATACGGGG TCTGGTGGGA CACCCAGGTT CTCCAGGAGC AGATGGGGCT 
CAGGGACGCC GGGGACCCCC AGGCCTCTTT GGGCAGAAAG GAGATGATGG AGTGAGGGGC 
TTCCCTGGCA CGATTGGTCC TCCTGGCCTA CAGGGAATGC CAGGCCCTGC TGGAGAAAAG 
GGAGAAGTTG GAGATGTAGG GTCCATGGGT CCCCATGGAG CTCCAGGCCC TCGGGGCCCC 
CAGGGCCCCA GCGGATCAGA GGGTCCTCCA GGGTTGCCTG GGGGAGTTGG TCAGCCCGGC 
GCTGTGGGTG AGAAGGGTGA GCCAGGAGAG CCTGGAGACC CAGGACCCCC AGGGACCCCA 
GGCGCCCCGG GCCCCAGGGG AGAAGTTGGT GAAAAGGGGG ATACAGGCCC ATCTGGGGCT 
GCTGGACCCC CAGGCAAGAA AGGCCCCCCT GGAGAGGATG GAGCAAAGGG GAATGTGGGC 
CCCGCAGGCC TCCCAGGAGA CCTAGGCCCC CCCGGAGACC CTGGAGTTTC GGGTCAAGGT 
GGCCCCCCAG GGGAGAAGGG AAACCCTGGA GATGTGGGGG GACCGGGTCC ACCTGGCGCT 
TCTGGGGAGC CTGGTGCCCC TGGGCCTCCT GGTAAAAGGG GTCCTTCTGG TCGCATGGGT 
CGAGAAGGCA GAGAAGGAGA GAAAGGTGCC AAGGGGGAAC CAGGTCCCGA TGGACCCCCA 
GGGAGGACAG GCCCAGTGGG GGCTGTAGGG CCCCCTGGAC GTGTTGGACC TGAGGGTCTT 
CGTGGGATCC CTGGACCTGT GGGTGAACCA GGCCTCCTGG GACCTCCTGG ACTGCTAGGC 
CCTCCTGGTC CCCTGGGGCC CTCTGGCCTA CCAGGGCTGA AGGGAGATGC TGGCCCCAAG 
GGAGAAAAGG GCCACATTGG ATTAACTGGT CTCATCGGCC CCCCTGGGGA GGCTGGTGAG 
AAAGGGGATC AGGGGTTGCC AGGAGTGCAA GGCCCCCCTG GCCTCCAGGG AGAACCGGGT 
TCCCCTGGCC CCATTGGCTC TCTGGGCCAC CCTGGGCCCC AAGGCGTGGT GGGTCCTCCA 
GGACAGAAAG GCTCAAAGGG GTCCCCAGGA ACCCAAGGTC CTCGTGGAGA CACCGGCCCC 
CCAGGTCCTC CTGGTCCCCC GGGTCCCTCT GCGGAGCTGC ATGGGCTGCG CCGGCGCCGA 
CGTTCCCTGC CAGGCTTGGG GACCCCGGAG GGCGGCCTGG AGGAGGTGCT GGCCTCGATG 
GCGTCGCTGA GCTTCGAGTT AGAGCAGCTG CGGCGCCCTC CCGGCACGGC TGAGCGCCCG 
GGCCTCGTGT GCAAAGAGTT GCACCGCAGC CATCCACATC TGCCGGATGG GGAATACTGG 
ATTGATCCCA ATCAAGGCTG CGCGCGGGAT GCACTCAGGG TTTTCTGTAA CTTCACGGCA 
GGAGGAGAAA CCTGCCTCTA CCCAGACAAG AAGTTTGAGA CAGTGAAACT GGCCTCTTGG 
TCCAAAGAAA AGCCAGGTGG CTGGTATAGC ACATTCCGTC GAGGGAAAAA GTTCTCCTAC 
GTGGATGCCG ATGGGGCCCC GGTGAGTGTG GTACAGCTGA CCTTCCTGAA GCTGCTGAGT 
GCCACGGCCC GCCAGAGCTT CACGTTCACC TGCCAGAACT CGGCCGCCTG GCTGGACGAA 
GCTGCAGGTG ACCACAGCCG CTCTGCCCGT TTCCTCGGGG CCAATGGGGA GGAGCTGTCC 
TTCAACCAGA CAGCAGCCGC CACCATCACG GTCCCCTACG ATGGCTGCAG GCTCCGGAAG 
GGACAGTCGA AGACCCTCTT TGAGTTCAAC TCCTCCCGAG TGGGATTTCT GCCCTTGTTG 
GATGTGGCAG CTGCTGACTT TGGCCAGACG AACCAGAAGT TTGGGTTTGA ACTGGGCCCT 
GTCTGCTTTA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL5A3 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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