SPAG17 gene cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

mRNA	Protein	Name
XM_012146169.2	XP_012001559.1	sperm-associated antigen 17 isoform X1
XM_004002384.4	XP_004002433.2	sperm-associated antigen 17 isoform X2
XM_027973609.1	XP_027829410.1	sperm-associated antigen 17 isoform X1
XM_012179498.2	XP_012034888.1	sperm-associated antigen 17 isoform X3
XM_012179500.1	XP_012034890.1	sperm-associated antigen 17 isoform X4
XM_012146171.2	XP_012001561.1	sperm-associated antigen 17 isoform X3
XM_015092205.1	XP_014947691.1	sperm-associated antigen 17 isoform X2
XM_004002384.3	XP_004002433.1	sperm-associated antigen 17 isoform X1
XM_012146172.1	XP_012001562.1	sperm-associated antigen 17 isoform X4
XM_015108726.1	XP_014964212.1	sperm-associated antigen 17 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv23606	XM_015108726.1 Latest version!	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv23607	XM_012146171.2 Latest version!	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv23608	XM_012146172.1 Latest version!	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm30420	XM_015092205.1 Latest version!	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm30421	XM_012179498.2 Latest version!	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm30422	XM_012179500.1 Latest version!	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm57766	XM_027973609.1 Latest version!	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv23605	XM_012146169.2 Latest version!	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm00812	XM_004002384.3 Latest version!	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm57767	XM_004002384.4 Latest version!	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

SPAG17 ( XM_015108726.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014964212.1 Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv23606
Clone ID Related Accession (Same CDS sequence)	XM_015108726.1
Accession Version	XM_015108726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	sperm-associated antigen 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942555.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGC TTGGCATTCC CATAAGCAGC GTGATTAAAA TATCTTCAGA GAATTATGAA 
CCTCTGCAGA CACACCTGGC AGCAGTTAGC CAACAGCAGG AAAGTTTTCT TCAGCCAGAA 
GAAATAGAAG CTGAAAAACT GAAGAAAGAG AATGGCATAA AAGAACTTGA AATCTTCTGG 
AAGTACTTGG AACCAATCCT GAATAACCAG AAACCAGAAA CAAATCTCTT TGATGTCGCT 
CAGCTTGAAT ACACGGTCAA GGCTGACAAG TTTCCTTCTG ACTGGTCAGA CAGCGAGATG 
TTGCTGGGAT TGGGCACCGA AATTTTTGAA AATGTTGCCT GTCTGATGTA CGACACCCTG 
GATTGGAAAA GGCAGCACCA GCACTATTTG GAAAGCATGC GGCTTATTAA CGTCCCACAG 
GTGGTTAGTG AGAAGTCTGT CTTTGAAGTG ACGTTGATTC CTGAGCCTCC ACAACCTGTT 
GTTGCAATCC CTGGAAAGAA GAAAACGCAA CCATATGAAG AGCCACAGAT TCCAGCTCCT 
CCTCCAGCAA CACTGCTACC ACCACCACCA GTGGCTTCCG TCATCACAAA TGAAGTAGAC 
ATGAGATACT ACAATGATTT GCTGAATCCA ATCCCAGAAG AATTCATTTC TGTGCCCTTG 
ATACTGCATT GTATGCTGGA ACAGGTTGTA GCAAGTGAAG AAGATCTTGT CCCACCAAGT 
CTTGTAGAGC TGCCTCCCAG GGCAGATGGG TTGGACTACA GAATAGCAGC TCACATCGTA 
TCCATCTTGC CCTCTCTCTG TCTGACAGAG AGGGAGAAAA AGAATCTCCA TGAAATATTC 
TTATCTGAAG AAGAAAATGA AAGCAAACCA GTGCAAACAG GCCCTCTCCT GCTTAACTAT 
CATGATGCCC ACGCCCACAA GAAGTACATG CTAAAGGACC TAAAGAATTT TGATCCAGTT 
CAAATTGAAC AAGAGATGCA GTCCAAGTTG CCACTGTGGG AATTCCTTAA ATTCCCTCTA 
CCTCCGCCAT GGAATAGCAC GAAACGTCTA GCTACAATTC ATGAGCTCAT GCACTTTTGT 
ACAAGTGAAG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAAGTGTT TACTTTTGAG 
AGCCTGAAAC TCTCTAAGGT TGATGAAGAT GGAAATCTAA AGCATTCTAA AATGATGTAT 
GGGACAGATT CTGAGATGTT CAACATACCA TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAAC AGTACATCAT GAAAATGAAT ACCCAAGAAG CCAAACAGCA AACAGATGGT 
GGAACCAAAG ACAGAATTTT ATTTCTGAAT CAGAATTGGT CAACACCTAT GCCAGATGAT 
GAGCTCAACA AAAAACCATC AGATTATCGC CAGCCTGGTA GTAACAAGAA GTCCTTAGAC 
TCTGATAATA GACAACCAAC AGAGCAGGAG ACAAACTTGA AGTCTCAGCT CCAACCTGAG 
ACTCTGGAGC AGACCATGAA CAATGAGATC AAAGATGAAG CGGTCACAAA TGCTGATCTT 
CTTGAAAAGA AACCAAAGAA GATGACTGTA GAAGCCGAGT TAGAGGACAT AAAGAAAACC 
CAGCAACGCA ATCTAATGGA CTGGAGTTTT ACTGAACATT TTAAACCAAA AGTTCTGCTT 
CAGGTTCTTC AAGAAGCCAG TCAGCAATAC AGGTGTGTTG ATTCTTATTA CCATACACAA 
GACAACTCGT TACTGTTGGT CTTTCATAAT CCAATGAATT TACAGCGTCT GCATCACGAA 
TATTGGAACG CTGGCCTTCA CTCTAATGTT GGATTCAGAA ATTATTTGGA ACTTGTTGCA 
AAATCCATTC AAGATTGGAT CATAAAAGAA GAAGCTATAT ATCAGGAAAC TAAAATGTCT 
GATGAAATCA ATAAAACTAA GTATGAGCTG GAAATAAAAT CTTCTGTTGC TACCAAAGTT 
TCTTCTACTA GCAAAATTTA TTCTATTAAG AAATCTAAAA GTAACAAAGG AATGAGCAAA 
ACAGAAATAT CAGATCAAGA AAAAGACAAA GAAAAAGAGA AGGAAAAGAT TAATTTTATT 
TTAGAAGGCT CTCTCAAGGC CTGGAAAGAA GAACAAGATC GGTTAGCTGA AGAGGAACGC 
TTAAAGGAAG AAAAAAAAGC AGAAAAGAAG AGTAAGGAGG CTGGTAAAAA GAAAGGCAAG 
GAAAAGCTAG AAAAAGAAGA TAGTAAGATT TTGAAGAAAA AATCTTTTAT AAAGGAGAAA 
TTGAAAGAAG AGCAAATGAA GATCCCGGAA GTAACAGAGG AGCCCCTCCA GGAACCAGAA 
CCTGAGATAG TTTACCCGTT TCATGGATAC AACATGGGAG AAATACCTGT GCAAATCTCA 
GGAACAAATT ATTATCTGTA TCCTTCCGAT GGAGGGCAAA TTGAAGTAGA AAAGACAACA 
TTTGAAAAAG GTCCAACAGT TATCAAAATG AAAGTGAAAA AGGACAAGCA CAATTTTTTA 
ATTCACTTAA GAGACCCTAG GAAAATTGTG GAAAAGGAGA AAAAAGAAGA GGATTATCCT 
TCAGAAGATG AGAAAGAAGA AGGAGGAGAG CAAAACTTCA AAGCAGAAGA ATCAAATAAA 
GAGAATAAAG CTGTCAGCAA GTTTGGATCT TTTTCTGCCA CCTTGGAAAA TGGAATCTGC 
CTCTCAATAA GTTACTATGG ATCAAGTGGA TCAGCACCAG AGGATAAGGA TCCTAACTTG 
GAATCTATGT TGAATATCCC ATCAGTACAT ACTCCAACAG TAATTCCTGT TACAGTGAGT 
ATTCCTCAAG GCAAGGGAAA AGGGAAAACA AAAGGCAAAG AAAAACCCAA AGAATCTCTT 
AAAGAAGAGG AACACCCAAA AGAAGAAGAG AAAAAGGAAG TAGAAGAACC AGAGCCTGTT 
TTACAAGAGA GTCCTCGTGT TCCCATCTTC CAAAACCTGA ATGTGTCTTG CCCCAATGGG 
CTCCAAGTGA CCTTCACTGG GCAAGAGTCC CCAGATCAAT ATATTGTAGG TGAGGAACCC 
ACTTGGGATA TCATGGTCCG ACAGAACTAC CCCCAGAAGG TGAGGCACTA TGAGTTCTAC 
AAAATGGTGA TGCCACCGCT GGAGCAGGAA GCATCGAGAG TTATCACCAG TCAAGGGACT 
GTTGTCAAAT ACATGTTGGA TGGATCCACA CAGATTCTCT TTGCAGATGG GACTGTGAGC 
AGTAGTCCCG ATTCAGGTCC TGTCAGTCTT CCTACTGACG TGCCGGCCAG TGCTCACAGT 
GCTGACTTGA TGGACACAAT TTCTCAGCAG AAATCAGAAA CAGCACCATC GGAGACTGTC 
ATCACAAAGA AAGGAAAAAT TCAGAAAAAT CAGTCATCTA TGGCACATAA AAATGAAAGC 
CATGAACTTC CCCCAGAAAC AGTTCAAACA ATAACTCCTA TGGATGTTCA CTTAGGCACC 
TGGTTTACAA CTACACCTCA AGGAAATCGG ATTGGCACCA AAGGATCAGA AAGAATAGCA 
GACTTGGCCC CATTTTTATC CTTTAAAGCC ACAGATCCTA TCAATGGAAC GGTTATGACA 
ACTCGAGAAG ACAAAGTGAT CATCATTGAA AAGAAAGATG GTACTCGGAT CGTGGATCAT 
GCTGATGGTA CCAGAATCAC AACCTTTTAT CAAGTTTATG AAGATCCGAC TATTTCTTCG 
GAGGATCAAG AGACAGCCGA AGGTCCTCAG AGGATCGCCA GGCAGGTGAA GTGCATGCGG 
ATTGAAAGCT CGCACTACGC CACCATCATC ACCAACTGCG AGGACAGTAG CTGCTGTGCC 
ACCTTTGCGG ACGGGACGTC CATCATTGCA AAGCCACAGG GAACGTACCA GGTGTTACCA 
CCAAACACAG GCTGTCTTCA TATTGCTAGG GACTGTTCAG CTATATATTG CCATGAATCA 
AGCAGTGACA TGTACCATCC TTTTCAAAAA CGTGAGCAGC TGAGAGCTAG CAGGTACATC 
ATGAAACACA CATCAGAGGT TATCTGCGAG GTCATGGATC CTGAAGGAAA TACCTTTCAG 
GTCATGGCTG ATGGTAGTGC ATCAACCATC CTACCTGAAA ACAACTTGGA AGATGATTTA 
AACGCAAAAG CTGAAGGTTA TGACAGTTTG TCATCCACTC ACCTTCATAA GAATCATCTG 
CAAATCTATG GTGAACATGT CCCCAGGTTT TTTGTTATCT ATGCTGATGG ATCAGGAGTG 
GAGCTTCTTC GGGACAGTGA CATAGAAGAG TACCTCTCCT TGGCATATGG AGAATCAACT 
ACCGTAGTTC TTCAAGAGCC AGTACAGGAA CAACCAGGTG CCCTGAGCAT CACCGTCCTT 
CGCCCTTTCC ATGAAGTGTC GCCGTGGCTA ATGAAGAAAG AATTAGATAC GATTGTTCCT 
CCTAATCTCC AGTCAAGGAC GTGGGAGACC TTTCCCTCAG TTCAGAAAAA AACTCCAGGA 
CCTCCGTTTG GCACTCAGAT TTGGAAGGGC CTCGACATTG GCTCCAAACA ATTAGTGAAC 
ACTCCAGCCC CTATACTGGA GAGCCCCAAT GTGCTACAGA TACGCCAGTT CATCCAGCAT 
GAGGTCATAA AGAATGAGGT GAAACTGAAA CTGCAGCTTT CCCTGAAGGA CTATATAAAC 
CATATTCTCA AGAAAGAAGA TGAGTTGCAG GAAATGACAG TTAAAGATTC CAGAACTGAG 
GAGGAGAGAG GCAACGCTGC AGATCTCCTC AAGCTGGTTA TGTCTTTCCC TAAAATGGAG 
GAAACTACAA AAAGTCATGT TACTGAAGTT GCAGCCCACC TAACTGATCT GTTTAAGCAG 
TCTTTGGCTA ATGCTATGGA ATGTCCACCA GAAACCTTTG AGACAGATTT CCTTGAAAAG 
AAATTGAGGC ACACAGAGAC GACAAAGCAC TGGAAAGAAA AGTTAGCTCA AAAGAGGAAG 
GAAATTGAAA AAACACAGAA CTATCTAAAG GAAATTAGGA GCAAAGCAAT ATCACCCTAC 
TTCAAATCTG AACTGAGCAA GCTTTATCAG TCTCAGTTTA ATTATCTGGA CACCCTTTCC 
AAAAACTTGC CTCCTTTTAC AAAGAAAGAT GAGGATGCAA GCAAAGAAGC CGCTCAAGAT 
ACATCCAGTC TTCCTCCAGA TTCCCAGAAA AATAAAGTTT CAAAACAGGA ATCCTCAGAT 
ATACTTAGTG TTTCAAAATC AGAGAACCTT GAAGATACCA AGGAGCCTGC TAGCCAAAAG 
CAAACTGAAA ATTTAACAGT ATCTATCAAA GAACCAGAAT CGTGTATGCC TCTGAAAATC 
CCAACCCAGT CAATGCTGCA GGATGTGGCA GGACAAGCAA GAAAACAGAA AGTGAGGCTA 
CCTCACTACT TGCTGAGTTC CAAGCCCAAG TCTCAGCCTC TCACAAAGAC GCAAGATTCT 
GTTGGAGGAA GAGTGAGAAC ATCCTCTGTG GCATCTGCTG CCATTAATAA TATAAATTCA 
TCTCCCTTTG GATTCCATCT TCTCCCACCC GTGGTGAAAT TTGGAGTGCT CGAAGAAGGA 
CACACGTATG CCACCACTGT AAAGCTCAAG AATGTTGGAG TGGACTTCTG CAGGTTTAGG 
GTGAAGCAGC CTCCACCCAG CACAGGACTG AAAGTGACTT ACAAACCTGG GCCTGTGGCG 
GCTGGTTTGC AGGCAGACCT GAGAGTGGAG TTATTCGCCA TGGCTGTCGG GGAGGATGGC 
ACCAAGGGAC TGGCTCACAT CTCTCACAAT ATAGAGATTA TGACTGAGCA TGACGTCCTG 
TTCTTACCTG TGGAAGCAAC AGTTTTAACA AGCAGCAATT ATGATAAGAG ACCAAAAGAT 
TTTCCCCAGG GAAAAGAAAA TCCCACGGTC CAGAGGATTT CTACAATTTC TTCCTCTGCA 
CTCGGAGTCT TCATGTCCCG TAAAATTTCT CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014964212.1
CDS	1383..7361
Translation	MTELGIPISSVIKISSENYEPLQTHLAAVSQQQESFLQPEEIEAEKLKKENGIKELEIFWKYLEPILNNQKPETNLFDVAQLEYTVKADKFPSDWSDSEMLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEEGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLPPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_015108726.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015108726.1

ORF Insert Sequence:

ATGACGGAGC TTGGCATTCC CATAAGCAGC GTGATTAAAA TATCTTCAGA GAATTATGAA 
CCTCTGCAGA CACACCTGGC AGCAGTTAGC CAACAGCAGG AAAGTTTTCT TCAGCCAGAA 
GAAATAGAAG CTGAAAAACT GAAGAAAGAG AATGGCATAA AAGAACTTGA AATCTTCTGG 
AAGTACTTGG AACCAATCCT GAATAACCAG AAACCAGAAA CAAATCTCTT TGATGTCGCT 
CAGCTTGAAT ACACGGTCAA GGCTGACAAG TTTCCTTCTG ACTGGTCAGA CAGCGAGATG 
TTGCTGGGAT TGGGCACCGA AATTTTTGAA AATGTTGCCT GTCTGATGTA CGACACCCTG 
GATTGGAAAA GGCAGCACCA GCACTATTTG GAAAGCATGC GGCTTATTAA CGTCCCACAG 
GTGGTTAGTG AGAAGTCTGT CTTTGAAGTG ACGTTGATTC CTGAGCCTCC ACAACCTGTT 
GTTGCAATCC CTGGAAAGAA GAAAACGCAA CCATATGAAG AGCCACAGAT TCCAGCTCCT 
CCTCCAGCAA CACTGCTACC ACCACCACCA GTGGCTTCCG TCATCACAAA TGAAGTAGAC 
ATGAGATACT ACAATGATTT GCTGAATCCA ATCCCAGAAG AATTCATTTC TGTGCCCTTG 
ATACTGCATT GTATGCTGGA ACAGGTTGTA GCAAGTGAAG AAGATCTTGT CCCACCAAGT 
CTTGTAGAGC TGCCTCCCAG GGCAGATGGG TTGGACTACA GAATAGCAGC TCACATCGTA 
TCCATCTTGC CCTCTCTCTG TCTGACAGAG AGGGAGAAAA AGAATCTCCA TGAAATATTC 
TTATCTGAAG AAGAAAATGA AAGCAAACCA GTGCAAACAG GCCCTCTCCT GCTTAACTAT 
CATGATGCCC ACGCCCACAA GAAGTACATG CTAAAGGACC TAAAGAATTT TGATCCAGTT 
CAAATTGAAC AAGAGATGCA GTCCAAGTTG CCACTGTGGG AATTCCTTAA ATTCCCTCTA 
CCTCCGCCAT GGAATAGCAC GAAACGTCTA GCTACAATTC ATGAGCTCAT GCACTTTTGT 
ACAAGTGAAG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAAGTGTT TACTTTTGAG 
AGCCTGAAAC TCTCTAAGGT TGATGAAGAT GGAAATCTAA AGCATTCTAA AATGATGTAT 
GGGACAGATT CTGAGATGTT CAACATACCA TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAAC AGTACATCAT GAAAATGAAT ACCCAAGAAG CCAAACAGCA AACAGATGGT 
GGAACCAAAG ACAGAATTTT ATTTCTGAAT CAGAATTGGT CAACACCTAT GCCAGATGAT 
GAGCTCAACA AAAAACCATC AGATTATCGC CAGCCTGGTA GTAACAAGAA GTCCTTAGAC 
TCTGATAATA GACAACCAAC AGAGCAGGAG ACAAACTTGA AGTCTCAGCT CCAACCTGAG 
ACTCTGGAGC AGACCATGAA CAATGAGATC AAAGATGAAG CGGTCACAAA TGCTGATCTT 
CTTGAAAAGA AACCAAAGAA GATGACTGTA GAAGCCGAGT TAGAGGACAT AAAGAAAACC 
CAGCAACGCA ATCTAATGGA CTGGAGTTTT ACTGAACATT TTAAACCAAA AGTTCTGCTT 
CAGGTTCTTC AAGAAGCCAG TCAGCAATAC AGGTGTGTTG ATTCTTATTA CCATACACAA 
GACAACTCGT TACTGTTGGT CTTTCATAAT CCAATGAATT TACAGCGTCT GCATCACGAA 
TATTGGAACG CTGGCCTTCA CTCTAATGTT GGATTCAGAA ATTATTTGGA ACTTGTTGCA 
AAATCCATTC AAGATTGGAT CATAAAAGAA GAAGCTATAT ATCAGGAAAC TAAAATGTCT 
GATGAAATCA ATAAAACTAA GTATGAGCTG GAAATAAAAT CTTCTGTTGC TACCAAAGTT 
TCTTCTACTA GCAAAATTTA TTCTATTAAG AAATCTAAAA GTAACAAAGG AATGAGCAAA 
ACAGAAATAT CAGATCAAGA AAAAGACAAA GAAAAAGAGA AGGAAAAGAT TAATTTTATT 
TTAGAAGGCT CTCTCAAGGC CTGGAAAGAA GAACAAGATC GGTTAGCTGA AGAGGAACGC 
TTAAAGGAAG AAAAAAAAGC AGAAAAGAAG AGTAAGGAGG CTGGTAAAAA GAAAGGCAAG 
GAAAAGCTAG AAAAAGAAGA TAGTAAGATT TTGAAGAAAA AATCTTTTAT AAAGGAGAAA 
TTGAAAGAAG AGCAAATGAA GATCCCGGAA GTAACAGAGG AGCCCCTCCA GGAACCAGAA 
CCTGAGATAG TTTACCCGTT TCATGGATAC AACATGGGAG AAATACCTGT GCAAATCTCA 
GGAACAAATT ATTATCTGTA TCCTTCCGAT GGAGGGCAAA TTGAAGTAGA AAAGACAACA 
TTTGAAAAAG GTCCAACAGT TATCAAAATG AAAGTGAAAA AGGACAAGCA CAATTTTTTA 
ATTCACTTAA GAGACCCTAG GAAAATTGTG GAAAAGGAGA AAAAAGAAGA GGATTATCCT 
TCAGAAGATG AGAAAGAAGA AGGAGGAGAG CAAAACTTCA AAGCAGAAGA ATCAAATAAA 
GAGAATAAAG CTGTCAGCAA GTTTGGATCT TTTTCTGCCA CCTTGGAAAA TGGAATCTGC 
CTCTCAATAA GTTACTATGG ATCAAGTGGA TCAGCACCAG AGGATAAGGA TCCTAACTTG 
GAATCTATGT TGAATATCCC ATCAGTACAT ACTCCAACAG TAATTCCTGT TACAGTGAGT 
ATTCCTCAAG GCAAGGGAAA AGGGAAAACA AAAGGCAAAG AAAAACCCAA AGAATCTCTT 
AAAGAAGAGG AACACCCAAA AGAAGAAGAG AAAAAGGAAG TAGAAGAACC AGAGCCTGTT 
TTACAAGAGA GTCCTCGTGT TCCCATCTTC CAAAACCTGA ATGTGTCTTG CCCCAATGGG 
CTCCAAGTGA CCTTCACTGG GCAAGAGTCC CCAGATCAAT ATATTGTAGG TGAGGAACCC 
ACTTGGGATA TCATGGTCCG ACAGAACTAC CCCCAGAAGG TGAGGCACTA TGAGTTCTAC 
AAAATGGTGA TGCCACCGCT GGAGCAGGAA GCATCGAGAG TTATCACCAG TCAAGGGACT 
GTTGTCAAAT ACATGTTGGA TGGATCCACA CAGATTCTCT TTGCAGATGG GACTGTGAGC 
AGTAGTCCCG ATTCAGGTCC TGTCAGTCTT CCTACTGACG TGCCGGCCAG TGCTCACAGT 
GCTGACTTGA TGGACACAAT TTCTCAGCAG AAATCAGAAA CAGCACCATC GGAGACTGTC 
ATCACAAAGA AAGGAAAAAT TCAGAAAAAT CAGTCATCTA TGGCACATAA AAATGAAAGC 
CATGAACTTC CCCCAGAAAC AGTTCAAACA ATAACTCCTA TGGATGTTCA CTTAGGCACC 
TGGTTTACAA CTACACCTCA AGGAAATCGG ATTGGCACCA AAGGATCAGA AAGAATAGCA 
GACTTGGCCC CATTTTTATC CTTTAAAGCC ACAGATCCTA TCAATGGAAC GGTTATGACA 
ACTCGAGAAG ACAAAGTGAT CATCATTGAA AAGAAAGATG GTACTCGGAT CGTGGATCAT 
GCTGATGGTA CCAGAATCAC AACCTTTTAT CAAGTTTATG AAGATCCGAC TATTTCTTCG 
GAGGATCAAG AGACAGCCGA AGGTCCTCAG AGGATCGCCA GGCAGGTGAA GTGCATGCGG 
ATTGAAAGCT CGCACTACGC CACCATCATC ACCAACTGCG AGGACAGTAG CTGCTGTGCC 
ACCTTTGCGG ACGGGACGTC CATCATTGCA AAGCCACAGG GAACGTACCA GGTGTTACCA 
CCAAACACAG GCTGTCTTCA TATTGCTAGG GACTGTTCAG CTATATATTG CCATGAATCA 
AGCAGTGACA TGTACCATCC TTTTCAAAAA CGTGAGCAGC TGAGAGCTAG CAGGTACATC 
ATGAAACACA CATCAGAGGT TATCTGCGAG GTCATGGATC CTGAAGGAAA TACCTTTCAG 
GTCATGGCTG ATGGTAGTGC ATCAACCATC CTACCTGAAA ACAACTTGGA AGATGATTTA 
AACGCAAAAG CTGAAGGTTA TGACAGTTTG TCATCCACTC ACCTTCATAA GAATCATCTG 
CAAATCTATG GTGAACATGT CCCCAGGTTT TTTGTTATCT ATGCTGATGG ATCAGGAGTG 
GAGCTTCTTC GGGACAGTGA CATAGAAGAG TACCTCTCCT TGGCATATGG AGAATCAACT 
ACCGTAGTTC TTCAAGAGCC AGTACAGGAA CAACCAGGTG CCCTGAGCAT CACCGTCCTT 
CGCCCTTTCC ATGAAGTGTC GCCGTGGCTA ATGAAGAAAG AATTAGATAC GATTGTTCCT 
CCTAATCTCC AGTCAAGGAC GTGGGAGACC TTTCCCTCAG TTCAGAAAAA AACTCCAGGA 
CCTCCGTTTG GCACTCAGAT TTGGAAGGGC CTCGACATTG GCTCCAAACA ATTAGTGAAC 
ACTCCAGCCC CTATACTGGA GAGCCCCAAT GTGCTACAGA TACGCCAGTT CATCCAGCAT 
GAGGTCATAA AGAATGAGGT GAAACTGAAA CTGCAGCTTT CCCTGAAGGA CTATATAAAC 
CATATTCTCA AGAAAGAAGA TGAGTTGCAG GAAATGACAG TTAAAGATTC CAGAACTGAG 
GAGGAGAGAG GCAACGCTGC AGATCTCCTC AAGCTGGTTA TGTCTTTCCC TAAAATGGAG 
GAAACTACAA AAAGTCATGT TACTGAAGTT GCAGCCCACC TAACTGATCT GTTTAAGCAG 
TCTTTGGCTA ATGCTATGGA ATGTCCACCA GAAACCTTTG AGACAGATTT CCTTGAAAAG 
AAATTGAGGC ACACAGAGAC GACAAAGCAC TGGAAAGAAA AGTTAGCTCA AAAGAGGAAG 
GAAATTGAAA AAACACAGAA CTATCTAAAG GAAATTAGGA GCAAAGCAAT ATCACCCTAC 
TTCAAATCTG AACTGAGCAA GCTTTATCAG TCTCAGTTTA ATTATCTGGA CACCCTTTCC 
AAAAACTTGC CTCCTTTTAC AAAGAAAGAT GAGGATGCAA GCAAAGAAGC CGCTCAAGAT 
ACATCCAGTC TTCCTCCAGA TTCCCAGAAA AATAAAGTTT CAAAACAGGA ATCCTCAGAT 
ATACTTAGTG TTTCAAAATC AGAGAACCTT GAAGATACCA AGGAGCCTGC TAGCCAAAAG 
CAAACTGAAA ATTTAACAGT ATCTATCAAA GAACCAGAAT CGTGTATGCC TCTGAAAATC 
CCAACCCAGT CAATGCTGCA GGATGTGGCA GGACAAGCAA GAAAACAGAA AGTGAGGCTA 
CCTCACTACT TGCTGAGTTC CAAGCCCAAG TCTCAGCCTC TCACAAAGAC GCAAGATTCT 
GTTGGAGGAA GAGTGAGAAC ATCCTCTGTG GCATCTGCTG CCATTAATAA TATAAATTCA 
TCTCCCTTTG GATTCCATCT TCTCCCACCC GTGGTGAAAT TTGGAGTGCT CGAAGAAGGA 
CACACGTATG CCACCACTGT AAAGCTCAAG AATGTTGGAG TGGACTTCTG CAGGTTTAGG 
GTGAAGCAGC CTCCACCCAG CACAGGACTG AAAGTGACTT ACAAACCTGG GCCTGTGGCG 
GCTGGTTTGC AGGCAGACCT GAGAGTGGAG TTATTCGCCA TGGCTGTCGG GGAGGATGGC 
ACCAAGGGAC TGGCTCACAT CTCTCACAAT ATAGAGATTA TGACTGAGCA TGACGTCCTG 
TTCTTACCTG TGGAAGCAAC AGTTTTAACA AGCAGCAATT ATGATAAGAG ACCAAAAGAT 
TTTCCCCAGG GAAAAGAAAA TCCCACGGTC CAGAGGATTT CTACAATTTC TTCCTCTGCA 
CTCGGAGTCT TCATGTCCCG TAAAATTTCT CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_012146171.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012001561.1 Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

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CloneID	OOv23607
Clone ID Related Accession (Same CDS sequence)	XM_012146171.2
Accession Version	XM_012146171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	sperm-associated antigen 17 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942555.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012146171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AGGCGGGACA ACCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGCCTCCA CAACCTGTTG TTGCAATCCC TGGAAAGAAG 
AAAACGCAAC CATATGAAGA GCCACAGATT CCAGCTCCTC CTCCAGCAAC ACTGCTACCA 
CCACCACCAG TGGCTTCCGT CATCACAAAT GAAGTAGACA TGAGATACTA CAATGATTTG 
CTGAATCCAA TCCCAGAAGA ATTCATTTCT GTGCCCTTGA TACTGCATTG TATGCTGGAA 
CAGGTTGTAG CAAGTGAAGA AGATCTTGTC CCACCAAGTC TTGTAGAGCT GCCTCCCAGG 
GCAGATGGGT TGGACTACAG AATAGCAGCT CACATCGTAT CCATCTTGCC CTCTCTCTGT 
CTGACAGAGA GGGAGAAAAA GAATCTCCAT GAAATATTCT TATCTGAAGA AGAAAATGAA 
AGCAAACCAG TGCAAACAGG CCCTCTCCTG CTTAACTATC ATGATGCCCA CGCCCACAAG 
AAGTACATGC TAAAGGACCT AAAGAATTTT GATCCAGTTC AAATTGAACA AGAGATGCAG 
TCCAAGTTGC CACTGTGGGA ATTCCTTAAA TTCCCTCTAC CTCCGCCATG GAATAGCACG 
AAACGTCTAG CTACAATTCA TGAGCTCATG CACTTTTGTA CAAGTGAAGT CTTGAGCTGG 
AATGAAGTAG AACGAGCCTT CAAAGTGTTT ACTTTTGAGA GCCTGAAACT CTCTAAGGTT 
GATGAAGATG GAAATCTAAA GCATTCTAAA ATGATGTATG GGACAGATTC TGAGATGTTC 
AACATACCAT GGGACAACCC TGCCAGATTT GCTAAACAGA TAAGGCAACA GTACATCATG 
AAAATGAATA CCCAAGAAGC CAAACAGCAA ACAGATGGTG GAACCAAAGA CAGAATTTTA 
TTTCTGAATC AGAATTGGTC AACACCTATG CCAGATGATG AGCTCAACAA AAAACCATCA 
GATTATCGCC AGCCTGGTAG TAACAAGAAG TCCTTAGACT CTGATAATAG ACAACCAACA 
GAGCAGGAGA CAAACTTGAA GTCTCAGCTC CAACCTGAGA CTCTGGAGCA GACCATGAAC 
AATGAGATCA AAGATGAAGC GGTCACAAAT GCTGATCTTC TTGAAAAGAA ACCAAAGAAG 
ATGACTGTAG AAGCCGAGTT AGAGGACATA AAGAAAACCC AGCAACGCAA TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCAAAA GTTCTGCTTC AGGTTCTTCA AGAAGCCAGT 
CAGCAATACA GGTGTGTTGA TTCTTATTAC CATACACAAG ACAACTCGTT ACTGTTGGTC 
TTTCATAATC CAATGAATTT ACAGCGTCTG CATCACGAAT ATTGGAACGC TGGCCTTCAC 
TCTAATGTTG GATTCAGAAA TTATTTGGAA CTTGTTGCAA AATCCATTCA AGATTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAAACT AAAATGTCTG ATGAAATCAA TAAAACTAAG 
TATGAGCTGG AAATAAAATC TTCTGTTGCT ACCAAAGTTT CTTCTACTAG CAAAATTTAT 
TCTATTAAGA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAAATATC AGATCAAGAA 
AAAGACAAAG AAAAAGAGAA GGAAAAGATT AATTTTATTT TAGAAGGCTC TCTCAAGGCC 
TGGAAAGAAG AACAAGATCG GTTAGCTGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAAAAGAAGA GTAAGGAGGC TGGTAAAAAG AAAGGCAAGG AAAAGCTAGA AAAAGAAGAT 
AGTAAGATTT TGAAGAAAAA ATCTTTTATA AAGGAGAAAT TGAAAGAAGA GCAAATGAAG 
ATCCCGGAAG TAACAGAGGA GCCCCTCCAG GAACCAGAAC CTGAGATAGT TTACCCGTTT 
CATGGATACA ACATGGGAGA AATACCTGTG CAAATCTCAG GAACAAATTA TTATCTGTAT 
CCTTCCGATG GAGGGCAAAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACAGTT 
ATCAAAATGA AAGTGAAAAA GGACAAGCAC AATTTTTTAA TTCACTTAAG AGACCCTAGG 
AAAATTGTGG AAAAGGAGAA AAAAGAAGAG GATTATCCTT CAGAAGATGA GAAAGAAGAA 
GGAGGAGAGC AAAACTTCAA AGCAGAAGAA TCAAATAAAG AGAATAAAGC TGTCAGCAAG 
TTTGGATCTT TTTCTGCCAC CTTGGAAAAT GGAATCTGCC TCTCAATAAG TTACTATGGA 
TCAAGTGGAT CAGCACCAGA GGATAAGGAT CCTAACTTGG AATCTATGTT GAATATCCCA 
TCAGTACATA CTCCAACAGT AATTCCTGTT ACAGTGAGTA TTCCTCAAGG CAAGGGAAAA 
GGGAAAACAA AAGGCAAAGA AAAACCCAAA GAATCTCTTA AAGAAGAGGA ACACCCAAAA 
GAAGAAGAGA AAAAGGAAGT AGAAGAACCA GAGCCTGTTT TACAAGAGAG TCCTCGTGTT 
CCCATCTTCC AAAACCTGAA TGTGTCTTGC CCCAATGGGC TCCAAGTGAC CTTCACTGGG 
CAAGAGTCCC CAGATCAATA TATTGTAGGT GAGGAACCCA CTTGGGATAT CATGGTCCGA 
CAGAACTACC CCCAGAAGGT GAGGCACTAT GAGTTCTACA AAATGGTGAT GCCACCGCTG 
GAGCAGGAAG CATCGAGAGT TATCACCAGT CAAGGGACTG TTGTCAAATA CATGTTGGAT 
GGATCCACAC AGATTCTCTT TGCAGATGGG ACTGTGAGCA GTAGTCCCGA TTCAGGTCCT 
GTCAGTCTTC CTACTGACGT GCCGGCCAGT GCTCACAGTG CTGACTTGAT GGACACAATT 
TCTCAGCAGA AATCAGAAAC AGCACCATCG GAGACTGTCA TCACAAAGAA AGGAAAAATT 
CAGAAAAATC AGTCATCTAT GGCACATAAA AATGAAAGCC ATGAACTTCC CCCAGAAACA 
GTTCAAACAA TAACTCCTAT GGATGTTCAC TTAGGCACCT GGTTTACAAC TACACCTCAA 
GGAAATCGGA TTGGCACCAA AGGATCAGAA AGAATAGCAG ACTTGGCCCC ATTTTTATCC 
TTTAAAGCCA CAGATCCTAT CAATGGAACG GTTATGACAA CTCGAGAAGA CAAAGTGATC 
ATCATTGAAA AGAAAGATGG TACTCGGATC GTGGATCATG CTGATGGTAC CAGAATCACA 
ACCTTTTATC AAGTTTATGA AGATCCGACT ATTTCTTCGG AGGATCAAGA GACAGCCGAA 
GGTCCTCAGA GGATCGCCAG GCAGGTGAAG TGCATGCGGA TTGAAAGCTC GCACTACGCC 
ACCATCATCA CCAACTGCGA GGACAGTAGC TGCTGTGCCA CCTTTGCGGA CGGGACGTCC 
ATCATTGCAA AGCCACAGGG AACGTACCAG GTGTTACCAC CAAACACAGG CTGTCTTCAT 
ATTGCTAGGG ACTGTTCAGC TATATATTGC CATGAATCAA GCAGTGACAT GTACCATCCT 
TTTCAAAAAC GTGAGCAGCT GAGAGCTAGC AGGTACATCA TGAAACACAC ATCAGAGGTT 
ATCTGCGAGG TCATGGATCC TGAAGGAAAT ACCTTTCAGG TCATGGCTGA TGGTAGTGCA 
TCAACCATCC TACCTGAAAA CAACTTGGAA GATGATTTAA ACGCAAAAGC TGAAGGTTAT 
GACAGTTTGT CATCCACTCA CCTTCATAAG AATCATCTGC AAATCTATGG TGAACATGTC 
CCCAGGTTTT TTGTTATCTA TGCTGATGGA TCAGGAGTGG AGCTTCTTCG GGACAGTGAC 
ATAGAAGAGT ACCTCTCCTT GGCATATGGA GAATCAACTA CCGTAGTTCT TCAAGAGCCA 
GTACAGGAAC AACCAGGTGC CCTGAGCATC ACCGTCCTTC GCCCTTTCCA TGAAGTGTCG 
CCGTGGCTAA TGAAGAAAGA ATTAGATACG ATTGTTCCTC CTAATCTCCA GTCAAGGACG 
TGGGAGACCT TTCCCTCAGT TCAGAAAAAA ACTCCAGGAC CTCCGTTTGG CACTCAGATT 
TGGAAGGGCC TCGACATTGG CTCCAAACAA TTAGTGAACA CTCCAGCCCC TATACTGGAG 
AGCCCCAATG TGCTACAGAT ACGCCAGTTC ATCCAGCATG AGGTCATAAA GAATGAGGTG 
AAACTGAAAC TGCAGCTTTC CCTGAAGGAC TATATAAACC ATATTCTCAA GAAAGAAGAT 
GAGTTGCAGG AAATGACAGT TAAAGATTCC AGAACTGAGG AGGAGAGAGG CAACGCTGCA 
GATCTCCTCA AGCTGGTTAT GTCTTTCCCT AAAATGGAGG AAACTACAAA AAGTCATGTT 
ACTGAAGTTG CAGCCCACCT AACTGATCTG TTTAAGCAGT CTTTGGCTAA TGCTATGGAA 
TGTCCACCAG AAACCTTTGA GACAGATTTC CTTGAAAAGA AATTGAGGCA CACAGAGACG 
ACAAAGCACT GGAAAGAAAA GTTAGCTCAA AAGAGGAAGG AAATTGAAAA AACACAGAAC 
TATCTAAAGG AAATTAGGAG CAAAGCAATA TCACCCTACT TCAAATCTGA ACTGAGCAAG 
CTTTATCAGT CTCAGTTTAA TTATCTGGAC ACCCTTTCCA AAAACTTGCC TCCTTTTACA 
AAGAAAGATG AGGATGCAAG CAAAGAAGCC GCTCAAGATA CATCCAGTCT TCCTCCAGAT 
TCCCAGAAAA ATAAAGTTTC AAAACAGGAA TCCTCAGATA TACTTAGTGT TTCAAAATCA 
GAGAACCTTG AAGATACCAA GGAGCCTGCT AGCCAAAAGC AAACTGAAAA TTTAACAGTA 
TCTATCAAAG AACCAGAATC GTGTATGCCT CTGAAAATCC CAACCCAGTC AATGCTGCAG 
GATGTGGCAG GACAAGCAAG AAAACAGAAA GTGAGGCTAC CTCACTACTT GCTGAGTTCC 
AAGCCCAAGT CTCAGCCTCT CACAAAGACG CAAGATTCTG TTGGAGGAAG AGTGAGAACA 
TCCTCTGTGG CATCTGCTGC CATTAATAAT ATAAATTCAT CTCCCTTTGG ATTCCATCTT 
CTCCCACCCG TGGTGAAATT TGGAGTGCTC GAAGAAGGAC ACACGTATGC CACCACTGTA 
AAGCTCAAGA ATGTTGGAGT GGACTTCTGC AGGTTTAGGG TGAAGCAGCC TCCACCCAGC 
ACAGGACTGA AAGTGACTTA CAAACCTGGG CCTGTGGCGG CTGGTTTGCA GGCAGACCTG 
AGAGTGGAGT TATTCGCCAT GGCTGTCGGG GAGGATGGCA CCAAGGGACT GGCTCACATC 
TCTCACAATA TAGAGATTAT GACTGAGCAT GACGTCCTGT TCTTACCTGT GGAAGCAACA 
GTTTTAACAA GCAGCAATTA TGATAAGAGA CCAAAAGATT TTCCCCAGGG AAAAGAAAAT 
CCCACGGTCC AGAGGATTTC TACAATTTCT TCCTCTGCAC TCGGAGTCTT CATGTCCCGT 
AAAATTTCTC CACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012001561.1
CDS	129..6086
Translation	MAPKKEKGGTTTTGSKIWEPSLIAAQFNQNDWKGSIAFVVGNRIEDEVLIHALTLAVQVPQRRLFSMVSWQDIVQQVNGSSAVFGSTSKKAKKPLGGNAPLYSEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKLKDQQRRESEKKPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEEGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLPPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_012146171.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012146171.2

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AGGCGGGACA ACCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGCCTCCA CAACCTGTTG TTGCAATCCC TGGAAAGAAG 
AAAACGCAAC CATATGAAGA GCCACAGATT CCAGCTCCTC CTCCAGCAAC ACTGCTACCA 
CCACCACCAG TGGCTTCCGT CATCACAAAT GAAGTAGACA TGAGATACTA CAATGATTTG 
CTGAATCCAA TCCCAGAAGA ATTCATTTCT GTGCCCTTGA TACTGCATTG TATGCTGGAA 
CAGGTTGTAG CAAGTGAAGA AGATCTTGTC CCACCAAGTC TTGTAGAGCT GCCTCCCAGG 
GCAGATGGGT TGGACTACAG AATAGCAGCT CACATCGTAT CCATCTTGCC CTCTCTCTGT 
CTGACAGAGA GGGAGAAAAA GAATCTCCAT GAAATATTCT TATCTGAAGA AGAAAATGAA 
AGCAAACCAG TGCAAACAGG CCCTCTCCTG CTTAACTATC ATGATGCCCA CGCCCACAAG 
AAGTACATGC TAAAGGACCT AAAGAATTTT GATCCAGTTC AAATTGAACA AGAGATGCAG 
TCCAAGTTGC CACTGTGGGA ATTCCTTAAA TTCCCTCTAC CTCCGCCATG GAATAGCACG 
AAACGTCTAG CTACAATTCA TGAGCTCATG CACTTTTGTA CAAGTGAAGT CTTGAGCTGG 
AATGAAGTAG AACGAGCCTT CAAAGTGTTT ACTTTTGAGA GCCTGAAACT CTCTAAGGTT 
GATGAAGATG GAAATCTAAA GCATTCTAAA ATGATGTATG GGACAGATTC TGAGATGTTC 
AACATACCAT GGGACAACCC TGCCAGATTT GCTAAACAGA TAAGGCAACA GTACATCATG 
AAAATGAATA CCCAAGAAGC CAAACAGCAA ACAGATGGTG GAACCAAAGA CAGAATTTTA 
TTTCTGAATC AGAATTGGTC AACACCTATG CCAGATGATG AGCTCAACAA AAAACCATCA 
GATTATCGCC AGCCTGGTAG TAACAAGAAG TCCTTAGACT CTGATAATAG ACAACCAACA 
GAGCAGGAGA CAAACTTGAA GTCTCAGCTC CAACCTGAGA CTCTGGAGCA GACCATGAAC 
AATGAGATCA AAGATGAAGC GGTCACAAAT GCTGATCTTC TTGAAAAGAA ACCAAAGAAG 
ATGACTGTAG AAGCCGAGTT AGAGGACATA AAGAAAACCC AGCAACGCAA TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCAAAA GTTCTGCTTC AGGTTCTTCA AGAAGCCAGT 
CAGCAATACA GGTGTGTTGA TTCTTATTAC CATACACAAG ACAACTCGTT ACTGTTGGTC 
TTTCATAATC CAATGAATTT ACAGCGTCTG CATCACGAAT ATTGGAACGC TGGCCTTCAC 
TCTAATGTTG GATTCAGAAA TTATTTGGAA CTTGTTGCAA AATCCATTCA AGATTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAAACT AAAATGTCTG ATGAAATCAA TAAAACTAAG 
TATGAGCTGG AAATAAAATC TTCTGTTGCT ACCAAAGTTT CTTCTACTAG CAAAATTTAT 
TCTATTAAGA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAAATATC AGATCAAGAA 
AAAGACAAAG AAAAAGAGAA GGAAAAGATT AATTTTATTT TAGAAGGCTC TCTCAAGGCC 
TGGAAAGAAG AACAAGATCG GTTAGCTGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAAAAGAAGA GTAAGGAGGC TGGTAAAAAG AAAGGCAAGG AAAAGCTAGA AAAAGAAGAT 
AGTAAGATTT TGAAGAAAAA ATCTTTTATA AAGGAGAAAT TGAAAGAAGA GCAAATGAAG 
ATCCCGGAAG TAACAGAGGA GCCCCTCCAG GAACCAGAAC CTGAGATAGT TTACCCGTTT 
CATGGATACA ACATGGGAGA AATACCTGTG CAAATCTCAG GAACAAATTA TTATCTGTAT 
CCTTCCGATG GAGGGCAAAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACAGTT 
ATCAAAATGA AAGTGAAAAA GGACAAGCAC AATTTTTTAA TTCACTTAAG AGACCCTAGG 
AAAATTGTGG AAAAGGAGAA AAAAGAAGAG GATTATCCTT CAGAAGATGA GAAAGAAGAA 
GGAGGAGAGC AAAACTTCAA AGCAGAAGAA TCAAATAAAG AGAATAAAGC TGTCAGCAAG 
TTTGGATCTT TTTCTGCCAC CTTGGAAAAT GGAATCTGCC TCTCAATAAG TTACTATGGA 
TCAAGTGGAT CAGCACCAGA GGATAAGGAT CCTAACTTGG AATCTATGTT GAATATCCCA 
TCAGTACATA CTCCAACAGT AATTCCTGTT ACAGTGAGTA TTCCTCAAGG CAAGGGAAAA 
GGGAAAACAA AAGGCAAAGA AAAACCCAAA GAATCTCTTA AAGAAGAGGA ACACCCAAAA 
GAAGAAGAGA AAAAGGAAGT AGAAGAACCA GAGCCTGTTT TACAAGAGAG TCCTCGTGTT 
CCCATCTTCC AAAACCTGAA TGTGTCTTGC CCCAATGGGC TCCAAGTGAC CTTCACTGGG 
CAAGAGTCCC CAGATCAATA TATTGTAGGT GAGGAACCCA CTTGGGATAT CATGGTCCGA 
CAGAACTACC CCCAGAAGGT GAGGCACTAT GAGTTCTACA AAATGGTGAT GCCACCGCTG 
GAGCAGGAAG CATCGAGAGT TATCACCAGT CAAGGGACTG TTGTCAAATA CATGTTGGAT 
GGATCCACAC AGATTCTCTT TGCAGATGGG ACTGTGAGCA GTAGTCCCGA TTCAGGTCCT 
GTCAGTCTTC CTACTGACGT GCCGGCCAGT GCTCACAGTG CTGACTTGAT GGACACAATT 
TCTCAGCAGA AATCAGAAAC AGCACCATCG GAGACTGTCA TCACAAAGAA AGGAAAAATT 
CAGAAAAATC AGTCATCTAT GGCACATAAA AATGAAAGCC ATGAACTTCC CCCAGAAACA 
GTTCAAACAA TAACTCCTAT GGATGTTCAC TTAGGCACCT GGTTTACAAC TACACCTCAA 
GGAAATCGGA TTGGCACCAA AGGATCAGAA AGAATAGCAG ACTTGGCCCC ATTTTTATCC 
TTTAAAGCCA CAGATCCTAT CAATGGAACG GTTATGACAA CTCGAGAAGA CAAAGTGATC 
ATCATTGAAA AGAAAGATGG TACTCGGATC GTGGATCATG CTGATGGTAC CAGAATCACA 
ACCTTTTATC AAGTTTATGA AGATCCGACT ATTTCTTCGG AGGATCAAGA GACAGCCGAA 
GGTCCTCAGA GGATCGCCAG GCAGGTGAAG TGCATGCGGA TTGAAAGCTC GCACTACGCC 
ACCATCATCA CCAACTGCGA GGACAGTAGC TGCTGTGCCA CCTTTGCGGA CGGGACGTCC 
ATCATTGCAA AGCCACAGGG AACGTACCAG GTGTTACCAC CAAACACAGG CTGTCTTCAT 
ATTGCTAGGG ACTGTTCAGC TATATATTGC CATGAATCAA GCAGTGACAT GTACCATCCT 
TTTCAAAAAC GTGAGCAGCT GAGAGCTAGC AGGTACATCA TGAAACACAC ATCAGAGGTT 
ATCTGCGAGG TCATGGATCC TGAAGGAAAT ACCTTTCAGG TCATGGCTGA TGGTAGTGCA 
TCAACCATCC TACCTGAAAA CAACTTGGAA GATGATTTAA ACGCAAAAGC TGAAGGTTAT 
GACAGTTTGT CATCCACTCA CCTTCATAAG AATCATCTGC AAATCTATGG TGAACATGTC 
CCCAGGTTTT TTGTTATCTA TGCTGATGGA TCAGGAGTGG AGCTTCTTCG GGACAGTGAC 
ATAGAAGAGT ACCTCTCCTT GGCATATGGA GAATCAACTA CCGTAGTTCT TCAAGAGCCA 
GTACAGGAAC AACCAGGTGC CCTGAGCATC ACCGTCCTTC GCCCTTTCCA TGAAGTGTCG 
CCGTGGCTAA TGAAGAAAGA ATTAGATACG ATTGTTCCTC CTAATCTCCA GTCAAGGACG 
TGGGAGACCT TTCCCTCAGT TCAGAAAAAA ACTCCAGGAC CTCCGTTTGG CACTCAGATT 
TGGAAGGGCC TCGACATTGG CTCCAAACAA TTAGTGAACA CTCCAGCCCC TATACTGGAG 
AGCCCCAATG TGCTACAGAT ACGCCAGTTC ATCCAGCATG AGGTCATAAA GAATGAGGTG 
AAACTGAAAC TGCAGCTTTC CCTGAAGGAC TATATAAACC ATATTCTCAA GAAAGAAGAT 
GAGTTGCAGG AAATGACAGT TAAAGATTCC AGAACTGAGG AGGAGAGAGG CAACGCTGCA 
GATCTCCTCA AGCTGGTTAT GTCTTTCCCT AAAATGGAGG AAACTACAAA AAGTCATGTT 
ACTGAAGTTG CAGCCCACCT AACTGATCTG TTTAAGCAGT CTTTGGCTAA TGCTATGGAA 
TGTCCACCAG AAACCTTTGA GACAGATTTC CTTGAAAAGA AATTGAGGCA CACAGAGACG 
ACAAAGCACT GGAAAGAAAA GTTAGCTCAA AAGAGGAAGG AAATTGAAAA AACACAGAAC 
TATCTAAAGG AAATTAGGAG CAAAGCAATA TCACCCTACT TCAAATCTGA ACTGAGCAAG 
CTTTATCAGT CTCAGTTTAA TTATCTGGAC ACCCTTTCCA AAAACTTGCC TCCTTTTACA 
AAGAAAGATG AGGATGCAAG CAAAGAAGCC GCTCAAGATA CATCCAGTCT TCCTCCAGAT 
TCCCAGAAAA ATAAAGTTTC AAAACAGGAA TCCTCAGATA TACTTAGTGT TTCAAAATCA 
GAGAACCTTG AAGATACCAA GGAGCCTGCT AGCCAAAAGC AAACTGAAAA TTTAACAGTA 
TCTATCAAAG AACCAGAATC GTGTATGCCT CTGAAAATCC CAACCCAGTC AATGCTGCAG 
GATGTGGCAG GACAAGCAAG AAAACAGAAA GTGAGGCTAC CTCACTACTT GCTGAGTTCC 
AAGCCCAAGT CTCAGCCTCT CACAAAGACG CAAGATTCTG TTGGAGGAAG AGTGAGAACA 
TCCTCTGTGG CATCTGCTGC CATTAATAAT ATAAATTCAT CTCCCTTTGG ATTCCATCTT 
CTCCCACCCG TGGTGAAATT TGGAGTGCTC GAAGAAGGAC ACACGTATGC CACCACTGTA 
AAGCTCAAGA ATGTTGGAGT GGACTTCTGC AGGTTTAGGG TGAAGCAGCC TCCACCCAGC 
ACAGGACTGA AAGTGACTTA CAAACCTGGG CCTGTGGCGG CTGGTTTGCA GGCAGACCTG 
AGAGTGGAGT TATTCGCCAT GGCTGTCGGG GAGGATGGCA CCAAGGGACT GGCTCACATC 
TCTCACAATA TAGAGATTAT GACTGAGCAT GACGTCCTGT TCTTACCTGT GGAAGCAACA 
GTTTTAACAA GCAGCAATTA TGATAAGAGA CCAAAAGATT TTCCCCAGGG AAAAGAAAAT 
CCCACGGTCC AGAGGATTTC TACAATTTCT TCCTCTGCAC TCGGAGTCTT CATGTCCCGT 
AAAATTTCTC CACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_012146172.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012001562.1 Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

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CloneID	OOv23608
Clone ID Related Accession (Same CDS sequence)	XM_012146172.1
Accession Version	XM_012146172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	sperm-associated antigen 17 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942555.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTCT TATTAGAAAT AGAAGCTGAA AAACTGAAGA AAGAGAATGG CATAAAAGAA 
CTTGAAATCT TCTGGAAGTA CTTGGAACCA ATCCTGAATA ACCAGAAACC AGAAACAAAT 
CTCTTTGATG TCGCTCAGCT TGAATACACG GTCAAGGCTG ACAAGTTTCC TTCTGACTGG 
TCAGACAGCG AGATGTTGCT GGGATTGGGC ACCGAAATTT TTGAAAATGT TGCCTGTCTG 
ATGTACGACA CCCTGGATTG GAAAAGGCAG CACCAGCACT ATTTGGAAAG CATGCGGCTT 
ATTAACGTCC CACAGGTGGT TAGTGAGAAG TCTGTCTTTG AAGTGACGTT GATTCCTGAG 
CCTCCACAAC CTGTTGTTGC AATCCCTGGA AAGAAGAAAA CGCAACCATA TGAAGAGCCA 
CAGATTCCAG CTCCTCCTCC AGCAACACTG CTACCACCAC CACCAGTGGC TTCCGTCATC 
ACAAATGAAG TAGACATGAG ATACTACAAT GATTTGCTGA ATCCAATCCC AGAAGAATTC 
ATTTCTGTGC CCTTGATACT GCATTGTATG CTGGAACAGG TTGTAGCAAG TGAAGAAGAT 
CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT CCCAGGGCAG ATGGGTTGGA CTACAGAATA 
GCAGCTCACA TCGTATCCAT CTTGCCCTCT CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT 
CTCCATGAAA TATTCTTATC TGAAGAAGAA AATGAAAGCA AACCAGTGCA AACAGGCCCT 
CTCCTGCTTA ACTATCATGA TGCCCACGCC CACAAGAAGT ACATGCTAAA GGACCTAAAG 
AATTTTGATC CAGTTCAAAT TGAACAAGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTC 
CTTAAATTCC CTCTACCTCC GCCATGGAAT AGCACGAAAC GTCTAGCTAC AATTCATGAG 
CTCATGCACT TTTGTACAAG TGAAGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAA 
GTGTTTACTT TTGAGAGCCT GAAACTCTCT AAGGTTGATG AAGATGGAAA TCTAAAGCAT 
TCTAAAATGA TGTATGGGAC AGATTCTGAG ATGTTCAACA TACCATGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAACAGTAC ATCATGAAAA TGAATACCCA AGAAGCCAAA 
CAGCAAACAG ATGGTGGAAC CAAAGACAGA ATTTTATTTC TGAATCAGAA TTGGTCAACA 
CCTATGCCAG ATGATGAGCT CAACAAAAAA CCATCAGATT ATCGCCAGCC TGGTAGTAAC 
AAGAAGTCCT TAGACTCTGA TAATAGACAA CCAACAGAGC AGGAGACAAA CTTGAAGTCT 
CAGCTCCAAC CTGAGACTCT GGAGCAGACC ATGAACAATG AGATCAAAGA TGAAGCGGTC 
ACAAATGCTG ATCTTCTTGA AAAGAAACCA AAGAAGATGA CTGTAGAAGC CGAGTTAGAG 
GACATAAAGA AAACCCAGCA ACGCAATCTA ATGGACTGGA GTTTTACTGA ACATTTTAAA 
CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA GCCAGTCAGC AATACAGGTG TGTTGATTCT 
TATTACCATA CACAAGACAA CTCGTTACTG TTGGTCTTTC ATAATCCAAT GAATTTACAG 
CGTCTGCATC ACGAATATTG GAACGCTGGC CTTCACTCTA ATGTTGGATT CAGAAATTAT 
TTGGAACTTG TTGCAAAATC CATTCAAGAT TGGATCATAA AAGAAGAAGC TATATATCAG 
GAAACTAAAA TGTCTGATGA AATCAATAAA ACTAAGTATG AGCTGGAAAT AAAATCTTCT 
GTTGCTACCA AAGTTTCTTC TACTAGCAAA ATTTATTCTA TTAAGAAATC TAAAAGTAAC 
AAAGGAATGA GCAAAACAGA AATATCAGAT CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA 
AAGATTAATT TTATTTTAGA AGGCTCTCTC AAGGCCTGGA AAGAAGAACA AGATCGGTTA 
GCTGAAGAGG AACGCTTAAA GGAAGAAAAA AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT 
AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA GAAGATAGTA AGATTTTGAA GAAAAAATCT 
TTTATAAAGG AGAAATTGAA AGAAGAGCAA ATGAAGATCC CGGAAGTAAC AGAGGAGCCC 
CTCCAGGAAC CAGAACCTGA GATAGTTTAC CCGTTTCATG GATACAACAT GGGAGAAATA 
CCTGTGCAAA TCTCAGGAAC AAATTATTAT CTGTATCCTT CCGATGGAGG GCAAATTGAA 
GTAGAAAAGA CAACATTTGA AAAAGGTCCA ACAGTTATCA AAATGAAAGT GAAAAAGGAC 
AAGCACAATT TTTTAATTCA CTTAAGAGAC CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA 
GAAGAGGATT ATCCTTCAGA AGATGAGAAA GAAGAAGGAG GAGAGCAAAA CTTCAAAGCA 
GAAGAATCAA ATAAAGAGAA TAAAGCTGTC AGCAAGTTTG GATCTTTTTC TGCCACCTTG 
GAAAATGGAA TCTGCCTCTC AATAAGTTAC TATGGATCAA GTGGATCAGC ACCAGAGGAT 
AAGGATCCTA ACTTGGAATC TATGTTGAAT ATCCCATCAG TACATACTCC AACAGTAATT 
CCTGTTACAG TGAGTATTCC TCAAGGCAAG GGAAAAGGGA AAACAAAAGG CAAAGAAAAA 
CCCAAAGAAT CTCTTAAAGA AGAGGAACAC CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA 
GAACCAGAGC CTGTTTTACA AGAGAGTCCT CGTGTTCCCA TCTTCCAAAA CCTGAATGTG 
TCTTGCCCCA ATGGGCTCCA AGTGACCTTC ACTGGGCAAG AGTCCCCAGA TCAATATATT 
GTAGGTGAGG AACCCACTTG GGATATCATG GTCCGACAGA ACTACCCCCA GAAGGTGAGG 
CACTATGAGT TCTACAAAAT GGTGATGCCA CCGCTGGAGC AGGAAGCATC GAGAGTTATC 
ACCAGTCAAG GGACTGTTGT CAAATACATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGGACTG TGAGCAGTAG TCCCGATTCA GGTCCTGTCA GTCTTCCTAC TGACGTGCCG 
GCCAGTGCTC ACAGTGCTGA CTTGATGGAC ACAATTTCTC AGCAGAAATC AGAAACAGCA 
CCATCGGAGA CTGTCATCAC AAAGAAAGGA AAAATTCAGA AAAATCAGTC ATCTATGGCA 
CATAAAAATG AAAGCCATGA ACTTCCCCCA GAAACAGTTC AAACAATAAC TCCTATGGAT 
GTTCACTTAG GCACCTGGTT TACAACTACA CCTCAAGGAA ATCGGATTGG CACCAAAGGA 
TCAGAAAGAA TAGCAGACTT GGCCCCATTT TTATCCTTTA AAGCCACAGA TCCTATCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTGATCATCA TTGAAAAGAA AGATGGTACT 
CGGATCGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CCGACTATTT CTTCGGAGGA TCAAGAGACA GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG 
GTGAAGTGCA TGCGGATTGA AAGCTCGCAC TACGCCACCA TCATCACCAA CTGCGAGGAC 
AGTAGCTGCT GTGCCACCTT TGCGGACGGG ACGTCCATCA TTGCAAAGCC ACAGGGAACG 
TACCAGGTGT TACCACCAAA CACAGGCTGT CTTCATATTG CTAGGGACTG TTCAGCTATA 
TATTGCCATG AATCAAGCAG TGACATGTAC CATCCTTTTC AAAAACGTGA GCAGCTGAGA 
GCTAGCAGGT ACATCATGAA ACACACATCA GAGGTTATCT GCGAGGTCAT GGATCCTGAA 
GGAAATACCT TTCAGGTCAT GGCTGATGGT AGTGCATCAA CCATCCTACC TGAAAACAAC 
TTGGAAGATG ATTTAAACGC AAAAGCTGAA GGTTATGACA GTTTGTCATC CACTCACCTT 
CATAAGAATC ATCTGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATCTATGCT 
GATGGATCAG GAGTGGAGCT TCTTCGGGAC AGTGACATAG AAGAGTACCT CTCCTTGGCA 
TATGGAGAAT CAACTACCGT AGTTCTTCAA GAGCCAGTAC AGGAACAACC AGGTGCCCTG 
AGCATCACCG TCCTTCGCCC TTTCCATGAA GTGTCGCCGT GGCTAATGAA GAAAGAATTA 
GATACGATTG TTCCTCCTAA TCTCCAGTCA AGGACGTGGG AGACCTTTCC CTCAGTTCAG 
AAAAAAACTC CAGGACCTCC GTTTGGCACT CAGATTTGGA AGGGCCTCGA CATTGGCTCC 
AAACAATTAG TGAACACTCC AGCCCCTATA CTGGAGAGCC CCAATGTGCT ACAGATACGC 
CAGTTCATCC AGCATGAGGT CATAAAGAAT GAGGTGAAAC TGAAACTGCA GCTTTCCCTG 
AAGGACTATA TAAACCATAT TCTCAAGAAA GAAGATGAGT TGCAGGAAAT GACAGTTAAA 
GATTCCAGAA CTGAGGAGGA GAGAGGCAAC GCTGCAGATC TCCTCAAGCT GGTTATGTCT 
TTCCCTAAAA TGGAGGAAAC TACAAAAAGT CATGTTACTG AAGTTGCAGC CCACCTAACT 
GATCTGTTTA AGCAGTCTTT GGCTAATGCT ATGGAATGTC CACCAGAAAC CTTTGAGACA 
GATTTCCTTG AAAAGAAATT GAGGCACACA GAGACGACAA AGCACTGGAA AGAAAAGTTA 
GCTCAAAAGA GGAAGGAAAT TGAAAAAACA CAGAACTATC TAAAGGAAAT TAGGAGCAAA 
GCAATATCAC CCTACTTCAA ATCTGAACTG AGCAAGCTTT ATCAGTCTCA GTTTAATTAT 
CTGGACACCC TTTCCAAAAA CTTGCCTCCT TTTACAAAGA AAGATGAGGA TGCAAGCAAA 
GAAGCCGCTC AAGATACATC CAGTCTTCCT CCAGATTCCC AGAAAAATAA AGTTTCAAAA 
CAGGAATCCT CAGATATACT TAGTGTTTCA AAATCAGAGA ACCTTGAAGA TACCAAGGAG 
CCTGCTAGCC AAAAGCAAAC TGAAAATTTA ACAGTATCTA TCAAAGAACC AGAATCGTGT 
ATGCCTCTGA AAATCCCAAC CCAGTCAATG CTGCAGGATG TGGCAGGACA AGCAAGAAAA 
CAGAAAGTGA GGCTACCTCA CTACTTGCTG AGTTCCAAGC CCAAGTCTCA GCCTCTCACA 
AAGACGCAAG ATTCTGTTGG AGGAAGAGTG AGAACATCCT CTGTGGCATC TGCTGCCATT 
AATAATATAA ATTCATCTCC CTTTGGATTC CATCTTCTCC CACCCGTGGT GAAATTTGGA 
GTGCTCGAAG AAGGACACAC GTATGCCACC ACTGTAAAGC TCAAGAATGT TGGAGTGGAC 
TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA CCCAGCACAG GACTGAAAGT GACTTACAAA 
CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA GACCTGAGAG TGGAGTTATT CGCCATGGCT 
GTCGGGGAGG ATGGCACCAA GGGACTGGCT CACATCTCTC ACAATATAGA GATTATGACT 
GAGCATGACG TCCTGTTCTT ACCTGTGGAA GCAACAGTTT TAACAAGCAG CAATTATGAT 
AAGAGACCAA AAGATTTTCC CCAGGGAAAA GAAAATCCCA CGGTCCAGAG GATTTCTACA 
ATTTCTTCCT CTGCACTCGG AGTCTTCATG TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012001562.1
CDS	218..6091
Translation	MLLLLEIEAEKLKKENGIKELEIFWKYLEPILNNQKPETNLFDVAQLEYTVKADKFPSDWSDSEMLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEEGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLPPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_012146172.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012146172.1

ORF Insert Sequence:

ATGTTGCTCT TATTAGAAAT AGAAGCTGAA AAACTGAAGA AAGAGAATGG CATAAAAGAA 
CTTGAAATCT TCTGGAAGTA CTTGGAACCA ATCCTGAATA ACCAGAAACC AGAAACAAAT 
CTCTTTGATG TCGCTCAGCT TGAATACACG GTCAAGGCTG ACAAGTTTCC TTCTGACTGG 
TCAGACAGCG AGATGTTGCT GGGATTGGGC ACCGAAATTT TTGAAAATGT TGCCTGTCTG 
ATGTACGACA CCCTGGATTG GAAAAGGCAG CACCAGCACT ATTTGGAAAG CATGCGGCTT 
ATTAACGTCC CACAGGTGGT TAGTGAGAAG TCTGTCTTTG AAGTGACGTT GATTCCTGAG 
CCTCCACAAC CTGTTGTTGC AATCCCTGGA AAGAAGAAAA CGCAACCATA TGAAGAGCCA 
CAGATTCCAG CTCCTCCTCC AGCAACACTG CTACCACCAC CACCAGTGGC TTCCGTCATC 
ACAAATGAAG TAGACATGAG ATACTACAAT GATTTGCTGA ATCCAATCCC AGAAGAATTC 
ATTTCTGTGC CCTTGATACT GCATTGTATG CTGGAACAGG TTGTAGCAAG TGAAGAAGAT 
CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT CCCAGGGCAG ATGGGTTGGA CTACAGAATA 
GCAGCTCACA TCGTATCCAT CTTGCCCTCT CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT 
CTCCATGAAA TATTCTTATC TGAAGAAGAA AATGAAAGCA AACCAGTGCA AACAGGCCCT 
CTCCTGCTTA ACTATCATGA TGCCCACGCC CACAAGAAGT ACATGCTAAA GGACCTAAAG 
AATTTTGATC CAGTTCAAAT TGAACAAGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTC 
CTTAAATTCC CTCTACCTCC GCCATGGAAT AGCACGAAAC GTCTAGCTAC AATTCATGAG 
CTCATGCACT TTTGTACAAG TGAAGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAA 
GTGTTTACTT TTGAGAGCCT GAAACTCTCT AAGGTTGATG AAGATGGAAA TCTAAAGCAT 
TCTAAAATGA TGTATGGGAC AGATTCTGAG ATGTTCAACA TACCATGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAACAGTAC ATCATGAAAA TGAATACCCA AGAAGCCAAA 
CAGCAAACAG ATGGTGGAAC CAAAGACAGA ATTTTATTTC TGAATCAGAA TTGGTCAACA 
CCTATGCCAG ATGATGAGCT CAACAAAAAA CCATCAGATT ATCGCCAGCC TGGTAGTAAC 
AAGAAGTCCT TAGACTCTGA TAATAGACAA CCAACAGAGC AGGAGACAAA CTTGAAGTCT 
CAGCTCCAAC CTGAGACTCT GGAGCAGACC ATGAACAATG AGATCAAAGA TGAAGCGGTC 
ACAAATGCTG ATCTTCTTGA AAAGAAACCA AAGAAGATGA CTGTAGAAGC CGAGTTAGAG 
GACATAAAGA AAACCCAGCA ACGCAATCTA ATGGACTGGA GTTTTACTGA ACATTTTAAA 
CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA GCCAGTCAGC AATACAGGTG TGTTGATTCT 
TATTACCATA CACAAGACAA CTCGTTACTG TTGGTCTTTC ATAATCCAAT GAATTTACAG 
CGTCTGCATC ACGAATATTG GAACGCTGGC CTTCACTCTA ATGTTGGATT CAGAAATTAT 
TTGGAACTTG TTGCAAAATC CATTCAAGAT TGGATCATAA AAGAAGAAGC TATATATCAG 
GAAACTAAAA TGTCTGATGA AATCAATAAA ACTAAGTATG AGCTGGAAAT AAAATCTTCT 
GTTGCTACCA AAGTTTCTTC TACTAGCAAA ATTTATTCTA TTAAGAAATC TAAAAGTAAC 
AAAGGAATGA GCAAAACAGA AATATCAGAT CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA 
AAGATTAATT TTATTTTAGA AGGCTCTCTC AAGGCCTGGA AAGAAGAACA AGATCGGTTA 
GCTGAAGAGG AACGCTTAAA GGAAGAAAAA AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT 
AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA GAAGATAGTA AGATTTTGAA GAAAAAATCT 
TTTATAAAGG AGAAATTGAA AGAAGAGCAA ATGAAGATCC CGGAAGTAAC AGAGGAGCCC 
CTCCAGGAAC CAGAACCTGA GATAGTTTAC CCGTTTCATG GATACAACAT GGGAGAAATA 
CCTGTGCAAA TCTCAGGAAC AAATTATTAT CTGTATCCTT CCGATGGAGG GCAAATTGAA 
GTAGAAAAGA CAACATTTGA AAAAGGTCCA ACAGTTATCA AAATGAAAGT GAAAAAGGAC 
AAGCACAATT TTTTAATTCA CTTAAGAGAC CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA 
GAAGAGGATT ATCCTTCAGA AGATGAGAAA GAAGAAGGAG GAGAGCAAAA CTTCAAAGCA 
GAAGAATCAA ATAAAGAGAA TAAAGCTGTC AGCAAGTTTG GATCTTTTTC TGCCACCTTG 
GAAAATGGAA TCTGCCTCTC AATAAGTTAC TATGGATCAA GTGGATCAGC ACCAGAGGAT 
AAGGATCCTA ACTTGGAATC TATGTTGAAT ATCCCATCAG TACATACTCC AACAGTAATT 
CCTGTTACAG TGAGTATTCC TCAAGGCAAG GGAAAAGGGA AAACAAAAGG CAAAGAAAAA 
CCCAAAGAAT CTCTTAAAGA AGAGGAACAC CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA 
GAACCAGAGC CTGTTTTACA AGAGAGTCCT CGTGTTCCCA TCTTCCAAAA CCTGAATGTG 
TCTTGCCCCA ATGGGCTCCA AGTGACCTTC ACTGGGCAAG AGTCCCCAGA TCAATATATT 
GTAGGTGAGG AACCCACTTG GGATATCATG GTCCGACAGA ACTACCCCCA GAAGGTGAGG 
CACTATGAGT TCTACAAAAT GGTGATGCCA CCGCTGGAGC AGGAAGCATC GAGAGTTATC 
ACCAGTCAAG GGACTGTTGT CAAATACATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGGACTG TGAGCAGTAG TCCCGATTCA GGTCCTGTCA GTCTTCCTAC TGACGTGCCG 
GCCAGTGCTC ACAGTGCTGA CTTGATGGAC ACAATTTCTC AGCAGAAATC AGAAACAGCA 
CCATCGGAGA CTGTCATCAC AAAGAAAGGA AAAATTCAGA AAAATCAGTC ATCTATGGCA 
CATAAAAATG AAAGCCATGA ACTTCCCCCA GAAACAGTTC AAACAATAAC TCCTATGGAT 
GTTCACTTAG GCACCTGGTT TACAACTACA CCTCAAGGAA ATCGGATTGG CACCAAAGGA 
TCAGAAAGAA TAGCAGACTT GGCCCCATTT TTATCCTTTA AAGCCACAGA TCCTATCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTGATCATCA TTGAAAAGAA AGATGGTACT 
CGGATCGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CCGACTATTT CTTCGGAGGA TCAAGAGACA GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG 
GTGAAGTGCA TGCGGATTGA AAGCTCGCAC TACGCCACCA TCATCACCAA CTGCGAGGAC 
AGTAGCTGCT GTGCCACCTT TGCGGACGGG ACGTCCATCA TTGCAAAGCC ACAGGGAACG 
TACCAGGTGT TACCACCAAA CACAGGCTGT CTTCATATTG CTAGGGACTG TTCAGCTATA 
TATTGCCATG AATCAAGCAG TGACATGTAC CATCCTTTTC AAAAACGTGA GCAGCTGAGA 
GCTAGCAGGT ACATCATGAA ACACACATCA GAGGTTATCT GCGAGGTCAT GGATCCTGAA 
GGAAATACCT TTCAGGTCAT GGCTGATGGT AGTGCATCAA CCATCCTACC TGAAAACAAC 
TTGGAAGATG ATTTAAACGC AAAAGCTGAA GGTTATGACA GTTTGTCATC CACTCACCTT 
CATAAGAATC ATCTGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATCTATGCT 
GATGGATCAG GAGTGGAGCT TCTTCGGGAC AGTGACATAG AAGAGTACCT CTCCTTGGCA 
TATGGAGAAT CAACTACCGT AGTTCTTCAA GAGCCAGTAC AGGAACAACC AGGTGCCCTG 
AGCATCACCG TCCTTCGCCC TTTCCATGAA GTGTCGCCGT GGCTAATGAA GAAAGAATTA 
GATACGATTG TTCCTCCTAA TCTCCAGTCA AGGACGTGGG AGACCTTTCC CTCAGTTCAG 
AAAAAAACTC CAGGACCTCC GTTTGGCACT CAGATTTGGA AGGGCCTCGA CATTGGCTCC 
AAACAATTAG TGAACACTCC AGCCCCTATA CTGGAGAGCC CCAATGTGCT ACAGATACGC 
CAGTTCATCC AGCATGAGGT CATAAAGAAT GAGGTGAAAC TGAAACTGCA GCTTTCCCTG 
AAGGACTATA TAAACCATAT TCTCAAGAAA GAAGATGAGT TGCAGGAAAT GACAGTTAAA 
GATTCCAGAA CTGAGGAGGA GAGAGGCAAC GCTGCAGATC TCCTCAAGCT GGTTATGTCT 
TTCCCTAAAA TGGAGGAAAC TACAAAAAGT CATGTTACTG AAGTTGCAGC CCACCTAACT 
GATCTGTTTA AGCAGTCTTT GGCTAATGCT ATGGAATGTC CACCAGAAAC CTTTGAGACA 
GATTTCCTTG AAAAGAAATT GAGGCACACA GAGACGACAA AGCACTGGAA AGAAAAGTTA 
GCTCAAAAGA GGAAGGAAAT TGAAAAAACA CAGAACTATC TAAAGGAAAT TAGGAGCAAA 
GCAATATCAC CCTACTTCAA ATCTGAACTG AGCAAGCTTT ATCAGTCTCA GTTTAATTAT 
CTGGACACCC TTTCCAAAAA CTTGCCTCCT TTTACAAAGA AAGATGAGGA TGCAAGCAAA 
GAAGCCGCTC AAGATACATC CAGTCTTCCT CCAGATTCCC AGAAAAATAA AGTTTCAAAA 
CAGGAATCCT CAGATATACT TAGTGTTTCA AAATCAGAGA ACCTTGAAGA TACCAAGGAG 
CCTGCTAGCC AAAAGCAAAC TGAAAATTTA ACAGTATCTA TCAAAGAACC AGAATCGTGT 
ATGCCTCTGA AAATCCCAAC CCAGTCAATG CTGCAGGATG TGGCAGGACA AGCAAGAAAA 
CAGAAAGTGA GGCTACCTCA CTACTTGCTG AGTTCCAAGC CCAAGTCTCA GCCTCTCACA 
AAGACGCAAG ATTCTGTTGG AGGAAGAGTG AGAACATCCT CTGTGGCATC TGCTGCCATT 
AATAATATAA ATTCATCTCC CTTTGGATTC CATCTTCTCC CACCCGTGGT GAAATTTGGA 
GTGCTCGAAG AAGGACACAC GTATGCCACC ACTGTAAAGC TCAAGAATGT TGGAGTGGAC 
TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA CCCAGCACAG GACTGAAAGT GACTTACAAA 
CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA GACCTGAGAG TGGAGTTATT CGCCATGGCT 
GTCGGGGAGG ATGGCACCAA GGGACTGGCT CACATCTCTC ACAATATAGA GATTATGACT 
GAGCATGACG TCCTGTTCTT ACCTGTGGAA GCAACAGTTT TAACAAGCAG CAATTATGAT 
AAGAGACCAA AAGATTTTCC CCAGGGAAAA GAAAATCCCA CGGTCCAGAG GATTTCTACA 
ATTTCTTCCT CTGCACTCGG AGTCTTCATG TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_015092205.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014947691.1 Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

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CloneID	OSm30420
Clone ID Related Accession (Same CDS sequence)	XM_015092205.1
Accession Version	XM_015092205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	sperm-associated antigen 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGC TTGGCATTCC CATAAGCAGC GTGATTAAAA TATCTTCAGA GAATTATGAA 
CCTCTGCAGA CACACCTGGC AGCAGTTAGC CAACAGCAGG AAAGTTTTCT TCAGCCAGAA 
GAAATAGAAG CTGAAAAACT GAAGAAAGAG AATGGCATAA AAGAACTTGA AATCTTCTGG 
AAGTACTTGG AACCAATCCT GAATAACCAG AAACCAGAAA CAAATCTCTT TGATGTCGCT 
CAGCTTGAAT ACACGGTCAA GGCTGACAAG TTTCCTTCTG ACTGGTCAGA CAGCGAGATG 
TTGCTGGGAT TGGGCACCGA AATTTTTGAA AATGTTGCCT GTCTGATGTA CGACACCCTG 
GATTGGAAAA GGCAGCACCA GCACTATTTG GAAAGCATGC GGCTTATTAA CGTCCCACAG 
GTGGTTAGTG AGAAGTCTGT CTTTGAAGTG ACGTTGATTC CTGAGCCTCC ACAACCTGTT 
GTTGCAATCC CTGGAAAGAA GAAAACGCAA CCATATGAAG AGCCACAGAT TCCAGCTCCT 
CCTCCAGCAA CACTGCTACC ACCACCACCA GTGGCTTCCG TCATCACAAA TGAAGTAGAC 
ATGAGATACT ACAATGATTT GCTGAATCCA ATCCCAGAAG AATTCATTTC TGTGCCCTTG 
ATACTGCATT GTATGCTGGA ACAGGTTGTA GCAAGTGAAG AAGATCTTGT CCCACCAAGT 
CTTGTAGAGC TGCCTCCCAG GGCAGATGGG TTGGACTACA GAATAGCAGC TCACATCGTA 
TCCATCTTGC CCTCTCTCTG TCTGACAGAG AGGGAGAAAA AGAATCTCCA TGAAATATTC 
TTATCTGAAG AAGAAAATGA AAGCAAACCA GTGCAAACAG GCCCTCTCCT GCTTAACTAT 
CATGATGCCC ACGCCCACAA GAAGTACATG CTAAAGGACC TAAAGAATTT TGATCCAGTT 
CAAATTGAAC AAGAGATGCA GTCCAAGTTG CCACTGTGGG AATTCCTTAA ATTCCCTCTG 
CCTCCGCCAT GGAATAGCAC GAAACGTCTA GCTACAATTC ATGAGCTCAT GCACTTTTGT 
ACAAGTGAAG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAAGTGTT TACTTTTGAG 
AGCCTGAAAC TCTCTAAGGT TGATGAAGAT GGAAATCTAA AGCATTCTAA AATGATGTAT 
GGGACAGATT CTGAGATGTT CAACATACCA TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAAC AGTACATCAT GAAAATGAAT ACCCAAGAAG CCAAACAGCA AACAGATGGT 
GGAACCAAAG ACAGAATTTT ATTTCTGAAT CAGAATTGGT CAACACCTAT GCCAGATGAT 
GAGCTCAACA AAAAACCATC AGATTATCGC CAGCCTGGTA GTAACAAGAA GTCCTTAGAC 
TCTGATAATA GACAACCAAC AGAGCAGGAG ACAAACTTGA AGTCTCAACT CCAACCTGAG 
ACTCTGGAGC AGACCATGAA CAATGAGATC AAAGATGAAG CGGTCACAAA TGCTGATCTT 
CTTGAAAAGA AACCAAAGAA GATGACTGTA GAAGCCGAGT TAGAGGACAT AAAGAAAACC 
CAGCAACGCA ATCTAATGGA CTGGAGTTTT ACTGAACATT TTAAACCAAA AGTTCTGCTT 
CAGGTTCTTC AAGAAGCCAG TCAGCAATAC AGGTGTGTTG ATTCTTATTA CCATACACAA 
GACAACTCGT TACTGTTGGT CTTTCATAAT CCAATGAATT TACAGCGTCT GCATCACGAA 
TATTGGAACG CTGGCCTTCA CTCTAATGTT GGATTCAGAA ATTATTTGGA ACTTGTTGCA 
AAATCCATTC AAGATTGGAT CATAAAAGAA GAAGCTATAT ATCAGGAAAC TAAAATGTCT 
GATGAAATCA ATAAAACTAA GTATGAGCTG GAAATAAAAT CTTCTGTTGC TACCAAAGTT 
TCTTCTACTA GCAAAATTTA TTCTATTAAG AAATCTAAAA GTAACAAAGG AATGAGCAAA 
ACAGAAATAT CAGATCAAGA AAAAGACAAA GAAAAAGAGA AGGAAAAGAT TAATTTTATT 
TTAGAAGGCT CTCTCAAGGC CTGGAAAGAA GAACAAGATC GGTTAGCTGA AGAGGAACGC 
TTAAAGGAAG AAAAAAAAGC AGAAAAGAAG AGTAAGGAGG CTGGTAAAAA GAAAGGCAAG 
GAAAAGCTAG AAAAAGAAGA TAGTAAGATT TTGAAGAAAA AATCTTTTAT AAAGGAGAAA 
TTGAAAGAAG AGCAAATGAA GATCCCGGAA GTAACAGAGG AGCCCCTCCA GGAACCAGAA 
CCTGAGATAG TTTACCCGTT TCATGGATAC AACATGGGAG AAATACCTGT GCAAATCTCA 
GGAACAAATT ATTATCTGTA TCCTTCCGAT GGAGGGCAAA TTGAAGTAGA AAAGACAACA 
TTTGAAAAAG GTCCAACAGT TATCAAAATG AAAGTGAAAA AGGACAAGCA CAATTTTTTA 
ATTCACTTAA GAGACCCTAG GAAAATTGTG GAAAAGGAGA AAAAAGAAGA GGATTATCCT 
TCAGAAGATG AGAAAGAAGG AGGAGGAGAG CAAAACTTCA AAGCAGAAGA ATCAAATAAA 
GAGAATAAAG CTGTCAGCAA GTTTGGATCT TTTTCTGCCA CCTTGGAAAA TGGAATCTGC 
CTCTCAATAA GTTACTATGG ATCAAGTGGA TCAGCACCAG AGGATAAGGA TCCTAACTTG 
GAATCTATGT TGAATATCCC ATCAGTACAT ACTCCAACAG TAATTCCTGT TACAGTGAGT 
ATTCCTCAAG GCAAGGGAAA AGGGAAAACA AAAGGCAAAG AAAAACCCAA AGAATCTCTT 
AAAGAAGAGG AGCACCCAAA AGAAGAAGAG AAAAAGGAAG TAGAAGAACC AGAGCCTGTT 
TTACAAGAGA GTCCTCGTGT TCCCATCTTC CAAAACCTGA ATGTGTCTTG CCCCAATGGG 
CTCCAAGTGA CCTTCACTGG GCAAGAGTCC CCAGATCAAT ATATTGTAGG TGAGGAACCC 
ACTTGGGATA TCATGGTCCG ACAGAACTAC CCCCAGAAGG TGAGGCACTA TGAGTTCTAC 
AAAATGGTGA TGCCACCGCT GGAGCAGGAA GCATCGAGAG TTATCACCAG TCAAGGGACT 
GTTGTCAAAT ACATGTTGGA TGGATCCACA CAGATTCTCT TTGCAGATGG GACTGTGAGC 
AGTAGTCCCG ATTCAGGTCC TGTCAGTCTT CCTACTGACG TGCCGGCCAG TGCTCACAGT 
GCTGACTTGA TGGACACAAT TTCTCAGCAG AAATCAGAAA CAGCACCATC GGAGACTGTC 
ATCACAAAGA AAGGAAAAAT TCAGAAAAAT CAGTCATCTA TGGCACATAA AAATGAAAGC 
CATGAACTTC CCCCAGAAAC AGTTCAAACA ATAACTCCTA TGGATGTTCA CTTAGGCACC 
TGGTTTACAA CTACACCTCA AGGAAATCGG ATTGGCACCA AAGGATCAGA AAGAATAGCA 
GACTTGGCCC CATTTTTATC CTTTAAAGCC ACAGATCCTA TCAATGGAAC GGTTATGACA 
ACTCGAGAAG ACAAAGTGAT CATCATTGAA AAGAAAGATG GTACTCGGAT CGTGGATCAT 
GCTGATGGTA CCAGAATCAC AACCTTTTAT CAAGTTTATG AAGATCCGAC TATTTCTTCG 
GAGGATCAAG AGACAGCCGA AGGTCCTCAG AGGATCGCCA GGCAGGTGAA GTGCATGCGG 
ATTGAAAGCT CACACTACGC CACCATCATC ACCAACTGCG AGGACAGTAG CTGCTGTGCC 
ACCTTTGCGG ACGGGACGTC CATCATTGCA AAGCCACAGG GAACGTACCA GGTGTTACCA 
CCAAACACAG GCTGTCTTCA TATTGCTAGG GACTGTTCAG CTATATATTG CCATGAATCA 
AGCAGTGACA TGTACCATCC TTTTCAAAAA CGTGAGCAGC TGAGAGCTAG CAGGTACATC 
ATGAAACACA CATCAGAGGT TATCTGTGAG GTCATGGATC CTGAAGGAAA TACCTTTCAG 
GTCATGGCTG ATGGTAGTGC ATCAACCATC CTACCTGAAA ACAACTTGGA AGATGATTTA 
AACGCAAAAG CTGAAGGTTA TGACAGTTTG TCATCCACTC ACCTTCATAA GAATCATCTG 
CAAATCTATG GTGAACATGT CCCCAGGTTT TTTGTTATCT ACGCTGATGG ATCAGGAGTG 
GAGCTTCTTC GGGACAGTGA CATAGAAGAG TACCTCTCCT TGGCATATGG AGAATCAACT 
ACCGTAGTTC TTCAAGAGCC AGTACAGGAA CAACCAGGTG CCCTGAGCAT CACCGTCCTT 
CGCCCTTTCC ATGAAGTGTC GCCGTGGCTA ATGAAGAAAG AATTAGATAC GATTGTTCCT 
CCTAATCTCC AGTCAAGGAC GTGGGAGACC TTTCCCTCAG TTCAGAAAAA AACTCCAGGA 
CCTCCGTTTG GCACTCAGAT TTGGAAGGGC CTCGACATTG GCTCCAAACA ATTAGTGAAC 
ACTCCAGCCC CTATACTGGA GAGCCCCAAT GTGCTACAGA TACGCCAGTT CATCCAGCAT 
GAGGTCATAA AGAATGAGGT GAAACTGAAA CTGCAGCTTT CCCTGAAGGA CTATATAAAC 
CATATTCTCA AGAAAGAAGA TGAGTTGCAG GAAATGACAG TTAAAGATTC CAGAACTGAG 
GAGGAGAGAG GCAACGCTGC AGATCTCCTC AAGCTGGTTA TGTCTTTCCC TAAAATGGAG 
GAAACTACAA AAAGTCATGT TACTGAAGTT GCAGCCCACC TAACTGATCT GTTTAAGCAG 
TCTTTGGCTA ATGCTATGGA ATGTCCACCA GAAACCTTTG AGACAGATTT CCTTGAAAAG 
AAATTGAGGC ACACAGAGAC GACAAAGCAC TGGAAAGAAA AGTTAGCTCA AAAGAGGAAG 
GAAATTGAAA AAACACAGAA CTATCTAAAG GAAATTAGGA GCAAAGCAAT ATCACCCTAC 
TTCAAATCTG AACTGAGCAA GCTTTATCAG TCTCAGTTTA ATTATCTGGA CACCCTTTCC 
AAAAACTTGC CTCCTTTTAC AAAGAAAGAT GAGGATGCAA GCAAAGAAGC TGCTCAAGAT 
ACATCCAGTC TTCTTCCAGA TTCCCAGAAA AATAAAGTTT CAAAACAGGA ATCCTCAGAT 
ATACTTAGTG TTTCAAAATC AGAGAACCTT GAAGATACCA AGGAGCCTGC TAGCCAAAAG 
CAAACTGAAA ATTTAACAGT ATCTATCAAA GAACCAGAAT CGTGTATGCC TCTGAAAATC 
CCAACCCAGT CAATGCTGCA GGATGTGGCA GGACAAGCAA GAAAACAGAA AGTGAGGCTA 
CCTCACTACT TGCTGAGTTC CAAGCCCAAG TCTCAGCCTC TCACAAAGAC GCAAGATTCT 
GTTGGAGGAA GAGTGAGAAC ATCCTCTGTG GCATCTGCTG CCATTAATAA TATAAATTCA 
TCTCCCTTTG GATTCCATCT TCTCCCACCC GTGGTGAAAT TTGGAGTGCT TGAAGAAGGA 
CACACGTATG CCACCACTGT AAAGCTCAAG AATGTTGGAG TGGACTTCTG CAGGTTTAGG 
GTGAAGCAGC CTCCACCCAG CACAGGACTG AAAGTGACTT ACAAACCTGG GCCTGTGGCG 
GCTGGTTTGC AGGCAGACCT GAGAGTGGAG TTATTCGCCA TGGCTGTCGG GGAGGATGGC 
ACCAAGGGAC TGGCTCACAT CTCTCACAAT ATAGAGATTA TGACTGAGCA TGACGTCCTG 
TTCTTACCTG TGGAAGCAAC AGTTTTAACA AGCAGCAATT ATGATAAGAG ACCAAAAGAT 
TTTCCCCAGG GAAAAGAAAA TCCCACGGTC CAGAGGATTT CTACAATTTC TTCCTCTGCA 
CTCGGAGTCT TCATGTCCCG TAAAATTTCT CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014947691.1
CDS	1353..7331
Translation	MTELGIPISSVIKISSENYEPLQTHLAAVSQQQESFLQPEEIEAEKLKKENGIKELEIFWKYLEPILNNQKPETNLFDVAQLEYTVKADKFPSDWSDSEMLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEGGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLLPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_015092205.1
Organism	Ovis aries(sheep)
Definition	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015092205.1

ORF Insert Sequence:

ATGACGGAGC TTGGCATTCC CATAAGCAGC GTGATTAAAA TATCTTCAGA GAATTATGAA 
CCTCTGCAGA CACACCTGGC AGCAGTTAGC CAACAGCAGG AAAGTTTTCT TCAGCCAGAA 
GAAATAGAAG CTGAAAAACT GAAGAAAGAG AATGGCATAA AAGAACTTGA AATCTTCTGG 
AAGTACTTGG AACCAATCCT GAATAACCAG AAACCAGAAA CAAATCTCTT TGATGTCGCT 
CAGCTTGAAT ACACGGTCAA GGCTGACAAG TTTCCTTCTG ACTGGTCAGA CAGCGAGATG 
TTGCTGGGAT TGGGCACCGA AATTTTTGAA AATGTTGCCT GTCTGATGTA CGACACCCTG 
GATTGGAAAA GGCAGCACCA GCACTATTTG GAAAGCATGC GGCTTATTAA CGTCCCACAG 
GTGGTTAGTG AGAAGTCTGT CTTTGAAGTG ACGTTGATTC CTGAGCCTCC ACAACCTGTT 
GTTGCAATCC CTGGAAAGAA GAAAACGCAA CCATATGAAG AGCCACAGAT TCCAGCTCCT 
CCTCCAGCAA CACTGCTACC ACCACCACCA GTGGCTTCCG TCATCACAAA TGAAGTAGAC 
ATGAGATACT ACAATGATTT GCTGAATCCA ATCCCAGAAG AATTCATTTC TGTGCCCTTG 
ATACTGCATT GTATGCTGGA ACAGGTTGTA GCAAGTGAAG AAGATCTTGT CCCACCAAGT 
CTTGTAGAGC TGCCTCCCAG GGCAGATGGG TTGGACTACA GAATAGCAGC TCACATCGTA 
TCCATCTTGC CCTCTCTCTG TCTGACAGAG AGGGAGAAAA AGAATCTCCA TGAAATATTC 
TTATCTGAAG AAGAAAATGA AAGCAAACCA GTGCAAACAG GCCCTCTCCT GCTTAACTAT 
CATGATGCCC ACGCCCACAA GAAGTACATG CTAAAGGACC TAAAGAATTT TGATCCAGTT 
CAAATTGAAC AAGAGATGCA GTCCAAGTTG CCACTGTGGG AATTCCTTAA ATTCCCTCTG 
CCTCCGCCAT GGAATAGCAC GAAACGTCTA GCTACAATTC ATGAGCTCAT GCACTTTTGT 
ACAAGTGAAG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAAGTGTT TACTTTTGAG 
AGCCTGAAAC TCTCTAAGGT TGATGAAGAT GGAAATCTAA AGCATTCTAA AATGATGTAT 
GGGACAGATT CTGAGATGTT CAACATACCA TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAAC AGTACATCAT GAAAATGAAT ACCCAAGAAG CCAAACAGCA AACAGATGGT 
GGAACCAAAG ACAGAATTTT ATTTCTGAAT CAGAATTGGT CAACACCTAT GCCAGATGAT 
GAGCTCAACA AAAAACCATC AGATTATCGC CAGCCTGGTA GTAACAAGAA GTCCTTAGAC 
TCTGATAATA GACAACCAAC AGAGCAGGAG ACAAACTTGA AGTCTCAACT CCAACCTGAG 
ACTCTGGAGC AGACCATGAA CAATGAGATC AAAGATGAAG CGGTCACAAA TGCTGATCTT 
CTTGAAAAGA AACCAAAGAA GATGACTGTA GAAGCCGAGT TAGAGGACAT AAAGAAAACC 
CAGCAACGCA ATCTAATGGA CTGGAGTTTT ACTGAACATT TTAAACCAAA AGTTCTGCTT 
CAGGTTCTTC AAGAAGCCAG TCAGCAATAC AGGTGTGTTG ATTCTTATTA CCATACACAA 
GACAACTCGT TACTGTTGGT CTTTCATAAT CCAATGAATT TACAGCGTCT GCATCACGAA 
TATTGGAACG CTGGCCTTCA CTCTAATGTT GGATTCAGAA ATTATTTGGA ACTTGTTGCA 
AAATCCATTC AAGATTGGAT CATAAAAGAA GAAGCTATAT ATCAGGAAAC TAAAATGTCT 
GATGAAATCA ATAAAACTAA GTATGAGCTG GAAATAAAAT CTTCTGTTGC TACCAAAGTT 
TCTTCTACTA GCAAAATTTA TTCTATTAAG AAATCTAAAA GTAACAAAGG AATGAGCAAA 
ACAGAAATAT CAGATCAAGA AAAAGACAAA GAAAAAGAGA AGGAAAAGAT TAATTTTATT 
TTAGAAGGCT CTCTCAAGGC CTGGAAAGAA GAACAAGATC GGTTAGCTGA AGAGGAACGC 
TTAAAGGAAG AAAAAAAAGC AGAAAAGAAG AGTAAGGAGG CTGGTAAAAA GAAAGGCAAG 
GAAAAGCTAG AAAAAGAAGA TAGTAAGATT TTGAAGAAAA AATCTTTTAT AAAGGAGAAA 
TTGAAAGAAG AGCAAATGAA GATCCCGGAA GTAACAGAGG AGCCCCTCCA GGAACCAGAA 
CCTGAGATAG TTTACCCGTT TCATGGATAC AACATGGGAG AAATACCTGT GCAAATCTCA 
GGAACAAATT ATTATCTGTA TCCTTCCGAT GGAGGGCAAA TTGAAGTAGA AAAGACAACA 
TTTGAAAAAG GTCCAACAGT TATCAAAATG AAAGTGAAAA AGGACAAGCA CAATTTTTTA 
ATTCACTTAA GAGACCCTAG GAAAATTGTG GAAAAGGAGA AAAAAGAAGA GGATTATCCT 
TCAGAAGATG AGAAAGAAGG AGGAGGAGAG CAAAACTTCA AAGCAGAAGA ATCAAATAAA 
GAGAATAAAG CTGTCAGCAA GTTTGGATCT TTTTCTGCCA CCTTGGAAAA TGGAATCTGC 
CTCTCAATAA GTTACTATGG ATCAAGTGGA TCAGCACCAG AGGATAAGGA TCCTAACTTG 
GAATCTATGT TGAATATCCC ATCAGTACAT ACTCCAACAG TAATTCCTGT TACAGTGAGT 
ATTCCTCAAG GCAAGGGAAA AGGGAAAACA AAAGGCAAAG AAAAACCCAA AGAATCTCTT 
AAAGAAGAGG AGCACCCAAA AGAAGAAGAG AAAAAGGAAG TAGAAGAACC AGAGCCTGTT 
TTACAAGAGA GTCCTCGTGT TCCCATCTTC CAAAACCTGA ATGTGTCTTG CCCCAATGGG 
CTCCAAGTGA CCTTCACTGG GCAAGAGTCC CCAGATCAAT ATATTGTAGG TGAGGAACCC 
ACTTGGGATA TCATGGTCCG ACAGAACTAC CCCCAGAAGG TGAGGCACTA TGAGTTCTAC 
AAAATGGTGA TGCCACCGCT GGAGCAGGAA GCATCGAGAG TTATCACCAG TCAAGGGACT 
GTTGTCAAAT ACATGTTGGA TGGATCCACA CAGATTCTCT TTGCAGATGG GACTGTGAGC 
AGTAGTCCCG ATTCAGGTCC TGTCAGTCTT CCTACTGACG TGCCGGCCAG TGCTCACAGT 
GCTGACTTGA TGGACACAAT TTCTCAGCAG AAATCAGAAA CAGCACCATC GGAGACTGTC 
ATCACAAAGA AAGGAAAAAT TCAGAAAAAT CAGTCATCTA TGGCACATAA AAATGAAAGC 
CATGAACTTC CCCCAGAAAC AGTTCAAACA ATAACTCCTA TGGATGTTCA CTTAGGCACC 
TGGTTTACAA CTACACCTCA AGGAAATCGG ATTGGCACCA AAGGATCAGA AAGAATAGCA 
GACTTGGCCC CATTTTTATC CTTTAAAGCC ACAGATCCTA TCAATGGAAC GGTTATGACA 
ACTCGAGAAG ACAAAGTGAT CATCATTGAA AAGAAAGATG GTACTCGGAT CGTGGATCAT 
GCTGATGGTA CCAGAATCAC AACCTTTTAT CAAGTTTATG AAGATCCGAC TATTTCTTCG 
GAGGATCAAG AGACAGCCGA AGGTCCTCAG AGGATCGCCA GGCAGGTGAA GTGCATGCGG 
ATTGAAAGCT CACACTACGC CACCATCATC ACCAACTGCG AGGACAGTAG CTGCTGTGCC 
ACCTTTGCGG ACGGGACGTC CATCATTGCA AAGCCACAGG GAACGTACCA GGTGTTACCA 
CCAAACACAG GCTGTCTTCA TATTGCTAGG GACTGTTCAG CTATATATTG CCATGAATCA 
AGCAGTGACA TGTACCATCC TTTTCAAAAA CGTGAGCAGC TGAGAGCTAG CAGGTACATC 
ATGAAACACA CATCAGAGGT TATCTGTGAG GTCATGGATC CTGAAGGAAA TACCTTTCAG 
GTCATGGCTG ATGGTAGTGC ATCAACCATC CTACCTGAAA ACAACTTGGA AGATGATTTA 
AACGCAAAAG CTGAAGGTTA TGACAGTTTG TCATCCACTC ACCTTCATAA GAATCATCTG 
CAAATCTATG GTGAACATGT CCCCAGGTTT TTTGTTATCT ACGCTGATGG ATCAGGAGTG 
GAGCTTCTTC GGGACAGTGA CATAGAAGAG TACCTCTCCT TGGCATATGG AGAATCAACT 
ACCGTAGTTC TTCAAGAGCC AGTACAGGAA CAACCAGGTG CCCTGAGCAT CACCGTCCTT 
CGCCCTTTCC ATGAAGTGTC GCCGTGGCTA ATGAAGAAAG AATTAGATAC GATTGTTCCT 
CCTAATCTCC AGTCAAGGAC GTGGGAGACC TTTCCCTCAG TTCAGAAAAA AACTCCAGGA 
CCTCCGTTTG GCACTCAGAT TTGGAAGGGC CTCGACATTG GCTCCAAACA ATTAGTGAAC 
ACTCCAGCCC CTATACTGGA GAGCCCCAAT GTGCTACAGA TACGCCAGTT CATCCAGCAT 
GAGGTCATAA AGAATGAGGT GAAACTGAAA CTGCAGCTTT CCCTGAAGGA CTATATAAAC 
CATATTCTCA AGAAAGAAGA TGAGTTGCAG GAAATGACAG TTAAAGATTC CAGAACTGAG 
GAGGAGAGAG GCAACGCTGC AGATCTCCTC AAGCTGGTTA TGTCTTTCCC TAAAATGGAG 
GAAACTACAA AAAGTCATGT TACTGAAGTT GCAGCCCACC TAACTGATCT GTTTAAGCAG 
TCTTTGGCTA ATGCTATGGA ATGTCCACCA GAAACCTTTG AGACAGATTT CCTTGAAAAG 
AAATTGAGGC ACACAGAGAC GACAAAGCAC TGGAAAGAAA AGTTAGCTCA AAAGAGGAAG 
GAAATTGAAA AAACACAGAA CTATCTAAAG GAAATTAGGA GCAAAGCAAT ATCACCCTAC 
TTCAAATCTG AACTGAGCAA GCTTTATCAG TCTCAGTTTA ATTATCTGGA CACCCTTTCC 
AAAAACTTGC CTCCTTTTAC AAAGAAAGAT GAGGATGCAA GCAAAGAAGC TGCTCAAGAT 
ACATCCAGTC TTCTTCCAGA TTCCCAGAAA AATAAAGTTT CAAAACAGGA ATCCTCAGAT 
ATACTTAGTG TTTCAAAATC AGAGAACCTT GAAGATACCA AGGAGCCTGC TAGCCAAAAG 
CAAACTGAAA ATTTAACAGT ATCTATCAAA GAACCAGAAT CGTGTATGCC TCTGAAAATC 
CCAACCCAGT CAATGCTGCA GGATGTGGCA GGACAAGCAA GAAAACAGAA AGTGAGGCTA 
CCTCACTACT TGCTGAGTTC CAAGCCCAAG TCTCAGCCTC TCACAAAGAC GCAAGATTCT 
GTTGGAGGAA GAGTGAGAAC ATCCTCTGTG GCATCTGCTG CCATTAATAA TATAAATTCA 
TCTCCCTTTG GATTCCATCT TCTCCCACCC GTGGTGAAAT TTGGAGTGCT TGAAGAAGGA 
CACACGTATG CCACCACTGT AAAGCTCAAG AATGTTGGAG TGGACTTCTG CAGGTTTAGG 
GTGAAGCAGC CTCCACCCAG CACAGGACTG AAAGTGACTT ACAAACCTGG GCCTGTGGCG 
GCTGGTTTGC AGGCAGACCT GAGAGTGGAG TTATTCGCCA TGGCTGTCGG GGAGGATGGC 
ACCAAGGGAC TGGCTCACAT CTCTCACAAT ATAGAGATTA TGACTGAGCA TGACGTCCTG 
TTCTTACCTG TGGAAGCAAC AGTTTTAACA AGCAGCAATT ATGATAAGAG ACCAAAAGAT 
TTTCCCCAGG GAAAAGAAAA TCCCACGGTC CAGAGGATTT CTACAATTTC TTCCTCTGCA 
CTCGGAGTCT TCATGTCCCG TAAAATTTCT CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_012179498.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034888.1 Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

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CloneID	OSm30421
Clone ID Related Accession (Same CDS sequence)	XM_012179498.2
Accession Version	XM_012179498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	sperm-associated antigen 17 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012179498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AGGCGGGACA GCCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGCCTCCA CAACCTGTTG TTGCAATCCC TGGAAAGAAG 
AAAACGCAAC CATATGAAGA GCCACAGATT CCAGCTCCTC CTCCAGCAAC ACTGCTACCA 
CCACCACCAG TGGCTTCCGT CATCACAAAT GAAGTAGACA TGAGATACTA CAATGATTTG 
CTGAATCCAA TCCCAGAAGA ATTCATTTCT GTGCCCTTGA TACTGCATTG TATGCTGGAA 
CAGGTTGTAG CAAGTGAAGA AGATCTTGTC CCACCAAGTC TTGTAGAGCT GCCTCCCAGG 
GCAGATGGGT TGGACTACAG AATAGCAGCT CACATCGTAT CCATCTTGCC CTCTCTCTGT 
CTGACAGAGA GGGAGAAAAA GAATCTCCAT GAAATATTCT TATCTGAAGA AGAAAATGAA 
AGCAAACCAG TGCAAACAGG CCCTCTCCTG CTTAACTATC ATGATGCCCA CGCCCACAAG 
AAGTACATGC TAAAGGACCT AAAGAATTTT GATCCAGTTC AAATTGAACA AGAGATGCAG 
TCCAAGTTGC CACTGTGGGA ATTCCTTAAA TTCCCTCTGC CTCCGCCATG GAATAGCACG 
AAACGTCTAG CTACAATTCA TGAGCTCATG CACTTTTGTA CAAGTGAAGT CTTGAGCTGG 
AATGAAGTAG AACGAGCCTT CAAAGTGTTT ACTTTTGAGA GCCTGAAACT CTCTAAGGTT 
GATGAAGATG GAAATCTAAA GCATTCTAAA ATGATGTATG GGACAGATTC TGAGATGTTC 
AACATACCAT GGGACAACCC TGCCAGATTT GCTAAACAGA TAAGGCAACA GTACATCATG 
AAAATGAATA CCCAAGAAGC CAAACAGCAA ACAGATGGTG GAACCAAAGA CAGAATTTTA 
TTTCTGAATC AGAATTGGTC AACACCTATG CCAGATGATG AGCTCAACAA AAAACCATCA 
GATTATCGCC AGCCTGGTAG TAACAAGAAG TCCTTAGACT CTGATAATAG ACAACCAACA 
GAGCAGGAGA CAAACTTGAA GTCTCAACTC CAACCTGAGA CTCTGGAGCA GACCATGAAC 
AATGAGATCA AAGATGAAGC GGTCACAAAT GCTGATCTTC TTGAAAAGAA ACCAAAGAAG 
ATGACTGTAG AAGCCGAGTT AGAGGACATA AAGAAAACCC AGCAACGCAA TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCAAAA GTTCTGCTTC AGGTTCTTCA AGAAGCCAGT 
CAGCAATACA GGTGTGTTGA TTCTTATTAC CATACACAAG ACAACTCGTT ACTGTTGGTC 
TTTCATAATC CAATGAATTT ACAGCGTCTG CATCACGAAT ATTGGAACGC TGGCCTTCAC 
TCTAATGTTG GATTCAGAAA TTATTTGGAA CTTGTTGCAA AATCCATTCA AGATTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAAACT AAAATGTCTG ATGAAATCAA TAAAACTAAG 
TATGAGCTGG AAATAAAATC TTCTGTTGCT ACCAAAGTTT CTTCTACTAG CAAAATTTAT 
TCTATTAAGA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAAATATC AGATCAAGAA 
AAAGACAAAG AAAAAGAGAA GGAAAAGATT AATTTTATTT TAGAAGGCTC TCTCAAGGCC 
TGGAAAGAAG AACAAGATCG GTTAGCTGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAAAAGAAGA GTAAGGAGGC TGGTAAAAAG AAAGGCAAGG AAAAGCTAGA AAAAGAAGAT 
AGTAAGATTT TGAAGAAAAA ATCTTTTATA AAGGAGAAAT TGAAAGAAGA GCAAATGAAG 
ATCCCGGAAG TAACAGAGGA GCCCCTCCAG GAACCAGAAC CTGAGATAGT TTACCCGTTT 
CATGGATACA ACATGGGAGA AATACCTGTG CAAATCTCAG GAACAAATTA TTATCTGTAT 
CCTTCCGATG GAGGGCAAAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACAGTT 
ATCAAAATGA AAGTGAAAAA GGACAAGCAC AATTTTTTAA TTCACTTAAG AGACCCTAGG 
AAAATTGTGG AAAAGGAGAA AAAAGAAGAG GATTATCCTT CAGAAGATGA GAAAGAAGGA 
GGAGGAGAGC AAAACTTCAA AGCAGAAGAA TCAAATAAAG AGAATAAAGC TGTCAGCAAG 
TTTGGATCTT TTTCTGCCAC CTTGGAAAAT GGAATCTGCC TCTCAATAAG TTACTATGGA 
TCAAGTGGAT CAGCACCAGA GGATAAGGAT CCTAACTTGG AATCTATGTT GAATATCCCA 
TCAGTACATA CTCCAACAGT AATTCCTGTT ACAGTGAGTA TTCCTCAAGG CAAGGGAAAA 
GGGAAAACAA AAGGCAAAGA AAAACCCAAA GAATCTCTTA AAGAAGAGGA GCACCCAAAA 
GAAGAAGAGA AAAAGGAAGT AGAAGAACCA GAGCCTGTTT TACAAGAGAG TCCTCGTGTT 
CCCATCTTCC AAAACCTGAA TGTGTCTTGC CCCAATGGGC TCCAAGTGAC CTTCACTGGG 
CAAGAGTCCC CAGATCAATA TATTGTAGGT GAGGAACCCA CTTGGGATAT CATGGTCCGA 
CAGAACTACC CCCAGAAGGT GAGGCACTAT GAGTTCTACA AAATGGTGAT GCCACCGCTG 
GAGCAGGAAG CATCGAGAGT TATCACCAGT CAAGGGACTG TTGTCAAATA CATGTTGGAT 
GGATCCACAC AGATTCTCTT TGCAGATGGG ACTGTGAGCA GTAGTCCCGA TTCAGGTCCT 
GTCAGTCTTC CTACTGACGT GCCGGCCAGT GCTCACAGTG CTGACTTGAT GGACACAATT 
TCTCAGCAGA AATCAGAAAC AGCACCATCG GAGACTGTCA TCACAAAGAA AGGAAAAATT 
CAGAAAAATC AGTCATCTAT GGCACATAAA AATGAAAGCC ATGAACTTCC CCCAGAAACA 
GTTCAAACAA TAACTCCTAT GGATGTTCAC TTAGGCACCT GGTTTACAAC TACACCTCAA 
GGAAATCGGA TTGGCACCAA AGGATCAGAA AGAATAGCAG ACTTGGCCCC ATTTTTATCC 
TTTAAAGCCA CAGATCCTAT CAATGGAACG GTTATGACAA CTCGAGAAGA CAAAGTGATC 
ATCATTGAAA AGAAAGATGG TACTCGGATC GTGGATCATG CTGATGGTAC CAGAATCACA 
ACCTTTTATC AAGTTTATGA AGATCCGACT ATTTCTTCGG AGGATCAAGA GACAGCCGAA 
GGTCCTCAGA GGATCGCCAG GCAGGTGAAG TGCATGCGGA TTGAAAGCTC ACACTACGCC 
ACCATCATCA CCAACTGCGA GGACAGTAGC TGCTGTGCCA CCTTTGCGGA CGGGACGTCC 
ATCATTGCAA AGCCACAGGG AACGTACCAG GTGTTACCAC CAAACACAGG CTGTCTTCAT 
ATTGCTAGGG ACTGTTCAGC TATATATTGC CATGAATCAA GCAGTGACAT GTACCATCCT 
TTTCAAAAAC GTGAGCAGCT GAGAGCTAGC AGGTACATCA TGAAACACAC ATCAGAGGTT 
ATCTGTGAGG TCATGGATCC TGAAGGAAAT ACCTTTCAGG TCATGGCTGA TGGTAGTGCA 
TCAACCATCC TACCTGAAAA CAACTTGGAA GATGATTTAA ACGCAAAAGC TGAAGGTTAT 
GACAGTTTGT CATCCACTCA CCTTCATAAG AATCATCTGC AAATCTATGG TGAACATGTC 
CCCAGGTTTT TTGTTATCTA CGCTGATGGA TCAGGAGTGG AGCTTCTTCG GGACAGTGAC 
ATAGAAGAGT ACCTCTCCTT GGCATATGGA GAATCAACTA CCGTAGTTCT TCAAGAGCCA 
GTACAGGAAC AACCAGGTGC CCTGAGCATC ACCGTCCTTC GCCCTTTCCA TGAAGTGTCG 
CCGTGGCTAA TGAAGAAAGA ATTAGATACG ATTGTTCCTC CTAATCTCCA GTCAAGGACG 
TGGGAGACCT TTCCCTCAGT TCAGAAAAAA ACTCCAGGAC CTCCGTTTGG CACTCAGATT 
TGGAAGGGCC TCGACATTGG CTCCAAACAA TTAGTGAACA CTCCAGCCCC TATACTGGAG 
AGCCCCAATG TGCTACAGAT ACGCCAGTTC ATCCAGCATG AGGTCATAAA GAATGAGGTG 
AAACTGAAAC TGCAGCTTTC CCTGAAGGAC TATATAAACC ATATTCTCAA GAAAGAAGAT 
GAGTTGCAGG AAATGACAGT TAAAGATTCC AGAACTGAGG AGGAGAGAGG CAACGCTGCA 
GATCTCCTCA AGCTGGTTAT GTCTTTCCCT AAAATGGAGG AAACTACAAA AAGTCATGTT 
ACTGAAGTTG CAGCCCACCT AACTGATCTG TTTAAGCAGT CTTTGGCTAA TGCTATGGAA 
TGTCCACCAG AAACCTTTGA GACAGATTTC CTTGAAAAGA AATTGAGGCA CACAGAGACG 
ACAAAGCACT GGAAAGAAAA GTTAGCTCAA AAGAGGAAGG AAATTGAAAA AACACAGAAC 
TATCTAAAGG AAATTAGGAG CAAAGCAATA TCACCCTACT TCAAATCTGA ACTGAGCAAG 
CTTTATCAGT CTCAGTTTAA TTATCTGGAC ACCCTTTCCA AAAACTTGCC TCCTTTTACA 
AAGAAAGATG AGGATGCAAG CAAAGAAGCT GCTCAAGATA CATCCAGTCT TCTTCCAGAT 
TCCCAGAAAA ATAAAGTTTC AAAACAGGAA TCCTCAGATA TACTTAGTGT TTCAAAATCA 
GAGAACCTTG AAGATACCAA GGAGCCTGCT AGCCAAAAGC AAACTGAAAA TTTAACAGTA 
TCTATCAAAG AACCAGAATC GTGTATGCCT CTGAAAATCC CAACCCAGTC AATGCTGCAG 
GATGTGGCAG GACAAGCAAG AAAACAGAAA GTGAGGCTAC CTCACTACTT GCTGAGTTCC 
AAGCCCAAGT CTCAGCCTCT CACAAAGACG CAAGATTCTG TTGGAGGAAG AGTGAGAACA 
TCCTCTGTGG CATCTGCTGC CATTAATAAT ATAAATTCAT CTCCCTTTGG ATTCCATCTT 
CTCCCACCCG TGGTGAAATT TGGAGTGCTT GAAGAAGGAC ACACGTATGC CACCACTGTA 
AAGCTCAAGA ATGTTGGAGT GGACTTCTGC AGGTTTAGGG TGAAGCAGCC TCCACCCAGC 
ACAGGACTGA AAGTGACTTA CAAACCTGGG CCTGTGGCGG CTGGTTTGCA GGCAGACCTG 
AGAGTGGAGT TATTCGCCAT GGCTGTCGGG GAGGATGGCA CCAAGGGACT GGCTCACATC 
TCTCACAATA TAGAGATTAT GACTGAGCAT GACGTCCTGT TCTTACCTGT GGAAGCAACA 
GTTTTAACAA GCAGCAATTA TGATAAGAGA CCAAAAGATT TTCCCCAGGG AAAAGAAAAT 
CCCACGGTCC AGAGGATTTC TACAATTTCT TCCTCTGCAC TCGGAGTCTT CATGTCCCGT 
AAAATTTCTC CACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034888.1
CDS	129..6086
Translation	MAPKKEKGGTATTGSKIWEPSLIAAQFNQNDWKGSIAFVVGNRIEDEVLIHALTLAVQVPQRRLFSMVSWQDIVQQVNGSSAVFGSTSKKAKKPLGGNAPLYSEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKLKDQQRRESEKKPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEGGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLLPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_012179498.2
Organism	Ovis aries(sheep)
Definition	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179498.2

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AGGCGGGACA GCCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGCCTCCA CAACCTGTTG TTGCAATCCC TGGAAAGAAG 
AAAACGCAAC CATATGAAGA GCCACAGATT CCAGCTCCTC CTCCAGCAAC ACTGCTACCA 
CCACCACCAG TGGCTTCCGT CATCACAAAT GAAGTAGACA TGAGATACTA CAATGATTTG 
CTGAATCCAA TCCCAGAAGA ATTCATTTCT GTGCCCTTGA TACTGCATTG TATGCTGGAA 
CAGGTTGTAG CAAGTGAAGA AGATCTTGTC CCACCAAGTC TTGTAGAGCT GCCTCCCAGG 
GCAGATGGGT TGGACTACAG AATAGCAGCT CACATCGTAT CCATCTTGCC CTCTCTCTGT 
CTGACAGAGA GGGAGAAAAA GAATCTCCAT GAAATATTCT TATCTGAAGA AGAAAATGAA 
AGCAAACCAG TGCAAACAGG CCCTCTCCTG CTTAACTATC ATGATGCCCA CGCCCACAAG 
AAGTACATGC TAAAGGACCT AAAGAATTTT GATCCAGTTC AAATTGAACA AGAGATGCAG 
TCCAAGTTGC CACTGTGGGA ATTCCTTAAA TTCCCTCTGC CTCCGCCATG GAATAGCACG 
AAACGTCTAG CTACAATTCA TGAGCTCATG CACTTTTGTA CAAGTGAAGT CTTGAGCTGG 
AATGAAGTAG AACGAGCCTT CAAAGTGTTT ACTTTTGAGA GCCTGAAACT CTCTAAGGTT 
GATGAAGATG GAAATCTAAA GCATTCTAAA ATGATGTATG GGACAGATTC TGAGATGTTC 
AACATACCAT GGGACAACCC TGCCAGATTT GCTAAACAGA TAAGGCAACA GTACATCATG 
AAAATGAATA CCCAAGAAGC CAAACAGCAA ACAGATGGTG GAACCAAAGA CAGAATTTTA 
TTTCTGAATC AGAATTGGTC AACACCTATG CCAGATGATG AGCTCAACAA AAAACCATCA 
GATTATCGCC AGCCTGGTAG TAACAAGAAG TCCTTAGACT CTGATAATAG ACAACCAACA 
GAGCAGGAGA CAAACTTGAA GTCTCAACTC CAACCTGAGA CTCTGGAGCA GACCATGAAC 
AATGAGATCA AAGATGAAGC GGTCACAAAT GCTGATCTTC TTGAAAAGAA ACCAAAGAAG 
ATGACTGTAG AAGCCGAGTT AGAGGACATA AAGAAAACCC AGCAACGCAA TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCAAAA GTTCTGCTTC AGGTTCTTCA AGAAGCCAGT 
CAGCAATACA GGTGTGTTGA TTCTTATTAC CATACACAAG ACAACTCGTT ACTGTTGGTC 
TTTCATAATC CAATGAATTT ACAGCGTCTG CATCACGAAT ATTGGAACGC TGGCCTTCAC 
TCTAATGTTG GATTCAGAAA TTATTTGGAA CTTGTTGCAA AATCCATTCA AGATTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAAACT AAAATGTCTG ATGAAATCAA TAAAACTAAG 
TATGAGCTGG AAATAAAATC TTCTGTTGCT ACCAAAGTTT CTTCTACTAG CAAAATTTAT 
TCTATTAAGA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAAATATC AGATCAAGAA 
AAAGACAAAG AAAAAGAGAA GGAAAAGATT AATTTTATTT TAGAAGGCTC TCTCAAGGCC 
TGGAAAGAAG AACAAGATCG GTTAGCTGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAAAAGAAGA GTAAGGAGGC TGGTAAAAAG AAAGGCAAGG AAAAGCTAGA AAAAGAAGAT 
AGTAAGATTT TGAAGAAAAA ATCTTTTATA AAGGAGAAAT TGAAAGAAGA GCAAATGAAG 
ATCCCGGAAG TAACAGAGGA GCCCCTCCAG GAACCAGAAC CTGAGATAGT TTACCCGTTT 
CATGGATACA ACATGGGAGA AATACCTGTG CAAATCTCAG GAACAAATTA TTATCTGTAT 
CCTTCCGATG GAGGGCAAAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACAGTT 
ATCAAAATGA AAGTGAAAAA GGACAAGCAC AATTTTTTAA TTCACTTAAG AGACCCTAGG 
AAAATTGTGG AAAAGGAGAA AAAAGAAGAG GATTATCCTT CAGAAGATGA GAAAGAAGGA 
GGAGGAGAGC AAAACTTCAA AGCAGAAGAA TCAAATAAAG AGAATAAAGC TGTCAGCAAG 
TTTGGATCTT TTTCTGCCAC CTTGGAAAAT GGAATCTGCC TCTCAATAAG TTACTATGGA 
TCAAGTGGAT CAGCACCAGA GGATAAGGAT CCTAACTTGG AATCTATGTT GAATATCCCA 
TCAGTACATA CTCCAACAGT AATTCCTGTT ACAGTGAGTA TTCCTCAAGG CAAGGGAAAA 
GGGAAAACAA AAGGCAAAGA AAAACCCAAA GAATCTCTTA AAGAAGAGGA GCACCCAAAA 
GAAGAAGAGA AAAAGGAAGT AGAAGAACCA GAGCCTGTTT TACAAGAGAG TCCTCGTGTT 
CCCATCTTCC AAAACCTGAA TGTGTCTTGC CCCAATGGGC TCCAAGTGAC CTTCACTGGG 
CAAGAGTCCC CAGATCAATA TATTGTAGGT GAGGAACCCA CTTGGGATAT CATGGTCCGA 
CAGAACTACC CCCAGAAGGT GAGGCACTAT GAGTTCTACA AAATGGTGAT GCCACCGCTG 
GAGCAGGAAG CATCGAGAGT TATCACCAGT CAAGGGACTG TTGTCAAATA CATGTTGGAT 
GGATCCACAC AGATTCTCTT TGCAGATGGG ACTGTGAGCA GTAGTCCCGA TTCAGGTCCT 
GTCAGTCTTC CTACTGACGT GCCGGCCAGT GCTCACAGTG CTGACTTGAT GGACACAATT 
TCTCAGCAGA AATCAGAAAC AGCACCATCG GAGACTGTCA TCACAAAGAA AGGAAAAATT 
CAGAAAAATC AGTCATCTAT GGCACATAAA AATGAAAGCC ATGAACTTCC CCCAGAAACA 
GTTCAAACAA TAACTCCTAT GGATGTTCAC TTAGGCACCT GGTTTACAAC TACACCTCAA 
GGAAATCGGA TTGGCACCAA AGGATCAGAA AGAATAGCAG ACTTGGCCCC ATTTTTATCC 
TTTAAAGCCA CAGATCCTAT CAATGGAACG GTTATGACAA CTCGAGAAGA CAAAGTGATC 
ATCATTGAAA AGAAAGATGG TACTCGGATC GTGGATCATG CTGATGGTAC CAGAATCACA 
ACCTTTTATC AAGTTTATGA AGATCCGACT ATTTCTTCGG AGGATCAAGA GACAGCCGAA 
GGTCCTCAGA GGATCGCCAG GCAGGTGAAG TGCATGCGGA TTGAAAGCTC ACACTACGCC 
ACCATCATCA CCAACTGCGA GGACAGTAGC TGCTGTGCCA CCTTTGCGGA CGGGACGTCC 
ATCATTGCAA AGCCACAGGG AACGTACCAG GTGTTACCAC CAAACACAGG CTGTCTTCAT 
ATTGCTAGGG ACTGTTCAGC TATATATTGC CATGAATCAA GCAGTGACAT GTACCATCCT 
TTTCAAAAAC GTGAGCAGCT GAGAGCTAGC AGGTACATCA TGAAACACAC ATCAGAGGTT 
ATCTGTGAGG TCATGGATCC TGAAGGAAAT ACCTTTCAGG TCATGGCTGA TGGTAGTGCA 
TCAACCATCC TACCTGAAAA CAACTTGGAA GATGATTTAA ACGCAAAAGC TGAAGGTTAT 
GACAGTTTGT CATCCACTCA CCTTCATAAG AATCATCTGC AAATCTATGG TGAACATGTC 
CCCAGGTTTT TTGTTATCTA CGCTGATGGA TCAGGAGTGG AGCTTCTTCG GGACAGTGAC 
ATAGAAGAGT ACCTCTCCTT GGCATATGGA GAATCAACTA CCGTAGTTCT TCAAGAGCCA 
GTACAGGAAC AACCAGGTGC CCTGAGCATC ACCGTCCTTC GCCCTTTCCA TGAAGTGTCG 
CCGTGGCTAA TGAAGAAAGA ATTAGATACG ATTGTTCCTC CTAATCTCCA GTCAAGGACG 
TGGGAGACCT TTCCCTCAGT TCAGAAAAAA ACTCCAGGAC CTCCGTTTGG CACTCAGATT 
TGGAAGGGCC TCGACATTGG CTCCAAACAA TTAGTGAACA CTCCAGCCCC TATACTGGAG 
AGCCCCAATG TGCTACAGAT ACGCCAGTTC ATCCAGCATG AGGTCATAAA GAATGAGGTG 
AAACTGAAAC TGCAGCTTTC CCTGAAGGAC TATATAAACC ATATTCTCAA GAAAGAAGAT 
GAGTTGCAGG AAATGACAGT TAAAGATTCC AGAACTGAGG AGGAGAGAGG CAACGCTGCA 
GATCTCCTCA AGCTGGTTAT GTCTTTCCCT AAAATGGAGG AAACTACAAA AAGTCATGTT 
ACTGAAGTTG CAGCCCACCT AACTGATCTG TTTAAGCAGT CTTTGGCTAA TGCTATGGAA 
TGTCCACCAG AAACCTTTGA GACAGATTTC CTTGAAAAGA AATTGAGGCA CACAGAGACG 
ACAAAGCACT GGAAAGAAAA GTTAGCTCAA AAGAGGAAGG AAATTGAAAA AACACAGAAC 
TATCTAAAGG AAATTAGGAG CAAAGCAATA TCACCCTACT TCAAATCTGA ACTGAGCAAG 
CTTTATCAGT CTCAGTTTAA TTATCTGGAC ACCCTTTCCA AAAACTTGCC TCCTTTTACA 
AAGAAAGATG AGGATGCAAG CAAAGAAGCT GCTCAAGATA CATCCAGTCT TCTTCCAGAT 
TCCCAGAAAA ATAAAGTTTC AAAACAGGAA TCCTCAGATA TACTTAGTGT TTCAAAATCA 
GAGAACCTTG AAGATACCAA GGAGCCTGCT AGCCAAAAGC AAACTGAAAA TTTAACAGTA 
TCTATCAAAG AACCAGAATC GTGTATGCCT CTGAAAATCC CAACCCAGTC AATGCTGCAG 
GATGTGGCAG GACAAGCAAG AAAACAGAAA GTGAGGCTAC CTCACTACTT GCTGAGTTCC 
AAGCCCAAGT CTCAGCCTCT CACAAAGACG CAAGATTCTG TTGGAGGAAG AGTGAGAACA 
TCCTCTGTGG CATCTGCTGC CATTAATAAT ATAAATTCAT CTCCCTTTGG ATTCCATCTT 
CTCCCACCCG TGGTGAAATT TGGAGTGCTT GAAGAAGGAC ACACGTATGC CACCACTGTA 
AAGCTCAAGA ATGTTGGAGT GGACTTCTGC AGGTTTAGGG TGAAGCAGCC TCCACCCAGC 
ACAGGACTGA AAGTGACTTA CAAACCTGGG CCTGTGGCGG CTGGTTTGCA GGCAGACCTG 
AGAGTGGAGT TATTCGCCAT GGCTGTCGGG GAGGATGGCA CCAAGGGACT GGCTCACATC 
TCTCACAATA TAGAGATTAT GACTGAGCAT GACGTCCTGT TCTTACCTGT GGAAGCAACA 
GTTTTAACAA GCAGCAATTA TGATAAGAGA CCAAAAGATT TTCCCCAGGG AAAAGAAAAT 
CCCACGGTCC AGAGGATTTC TACAATTTCT TCCTCTGCAC TCGGAGTCTT CATGTCCCGT 
AAAATTTCTC CACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_012179500.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012034890.1 Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSm30422
Clone ID Related Accession (Same CDS sequence)	XM_012179500.1
Accession Version	XM_012179500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	sperm-associated antigen 17 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTCT TATTAGAAAT AGAAGCTGAA AAACTGAAGA AAGAGAATGG CATAAAAGAA 
CTTGAAATCT TCTGGAAGTA CTTGGAACCA ATCCTGAATA ACCAGAAACC AGAAACAAAT 
CTCTTTGATG TCGCTCAGCT TGAATACACG GTCAAGGCTG ACAAGTTTCC TTCTGACTGG 
TCAGACAGCG AGATGTTGCT GGGATTGGGC ACCGAAATTT TTGAAAATGT TGCCTGTCTG 
ATGTACGACA CCCTGGATTG GAAAAGGCAG CACCAGCACT ATTTGGAAAG CATGCGGCTT 
ATTAACGTCC CACAGGTGGT TAGTGAGAAG TCTGTCTTTG AAGTGACGTT GATTCCTGAG 
CCTCCACAAC CTGTTGTTGC AATCCCTGGA AAGAAGAAAA CGCAACCATA TGAAGAGCCA 
CAGATTCCAG CTCCTCCTCC AGCAACACTG CTACCACCAC CACCAGTGGC TTCCGTCATC 
ACAAATGAAG TAGACATGAG ATACTACAAT GATTTGCTGA ATCCAATCCC AGAAGAATTC 
ATTTCTGTGC CCTTGATACT GCATTGTATG CTGGAACAGG TTGTAGCAAG TGAAGAAGAT 
CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT CCCAGGGCAG ATGGGTTGGA CTACAGAATA 
GCAGCTCACA TCGTATCCAT CTTGCCCTCT CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT 
CTCCATGAAA TATTCTTATC TGAAGAAGAA AATGAAAGCA AACCAGTGCA AACAGGCCCT 
CTCCTGCTTA ACTATCATGA TGCCCACGCC CACAAGAAGT ACATGCTAAA GGACCTAAAG 
AATTTTGATC CAGTTCAAAT TGAACAAGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTC 
CTTAAATTCC CTCTGCCTCC GCCATGGAAT AGCACGAAAC GTCTAGCTAC AATTCATGAG 
CTCATGCACT TTTGTACAAG TGAAGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAA 
GTGTTTACTT TTGAGAGCCT GAAACTCTCT AAGGTTGATG AAGATGGAAA TCTAAAGCAT 
TCTAAAATGA TGTATGGGAC AGATTCTGAG ATGTTCAACA TACCATGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAACAGTAC ATCATGAAAA TGAATACCCA AGAAGCCAAA 
CAGCAAACAG ATGGTGGAAC CAAAGACAGA ATTTTATTTC TGAATCAGAA TTGGTCAACA 
CCTATGCCAG ATGATGAGCT CAACAAAAAA CCATCAGATT ATCGCCAGCC TGGTAGTAAC 
AAGAAGTCCT TAGACTCTGA TAATAGACAA CCAACAGAGC AGGAGACAAA CTTGAAGTCT 
CAACTCCAAC CTGAGACTCT GGAGCAGACC ATGAACAATG AGATCAAAGA TGAAGCGGTC 
ACAAATGCTG ATCTTCTTGA AAAGAAACCA AAGAAGATGA CTGTAGAAGC CGAGTTAGAG 
GACATAAAGA AAACCCAGCA ACGCAATCTA ATGGACTGGA GTTTTACTGA ACATTTTAAA 
CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA GCCAGTCAGC AATACAGGTG TGTTGATTCT 
TATTACCATA CACAAGACAA CTCGTTACTG TTGGTCTTTC ATAATCCAAT GAATTTACAG 
CGTCTGCATC ACGAATATTG GAACGCTGGC CTTCACTCTA ATGTTGGATT CAGAAATTAT 
TTGGAACTTG TTGCAAAATC CATTCAAGAT TGGATCATAA AAGAAGAAGC TATATATCAG 
GAAACTAAAA TGTCTGATGA AATCAATAAA ACTAAGTATG AGCTGGAAAT AAAATCTTCT 
GTTGCTACCA AAGTTTCTTC TACTAGCAAA ATTTATTCTA TTAAGAAATC TAAAAGTAAC 
AAAGGAATGA GCAAAACAGA AATATCAGAT CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA 
AAGATTAATT TTATTTTAGA AGGCTCTCTC AAGGCCTGGA AAGAAGAACA AGATCGGTTA 
GCTGAAGAGG AACGCTTAAA GGAAGAAAAA AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT 
AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA GAAGATAGTA AGATTTTGAA GAAAAAATCT 
TTTATAAAGG AGAAATTGAA AGAAGAGCAA ATGAAGATCC CGGAAGTAAC AGAGGAGCCC 
CTCCAGGAAC CAGAACCTGA GATAGTTTAC CCGTTTCATG GATACAACAT GGGAGAAATA 
CCTGTGCAAA TCTCAGGAAC AAATTATTAT CTGTATCCTT CCGATGGAGG GCAAATTGAA 
GTAGAAAAGA CAACATTTGA AAAAGGTCCA ACAGTTATCA AAATGAAAGT GAAAAAGGAC 
AAGCACAATT TTTTAATTCA CTTAAGAGAC CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA 
GAAGAGGATT ATCCTTCAGA AGATGAGAAA GAAGGAGGAG GAGAGCAAAA CTTCAAAGCA 
GAAGAATCAA ATAAAGAGAA TAAAGCTGTC AGCAAGTTTG GATCTTTTTC TGCCACCTTG 
GAAAATGGAA TCTGCCTCTC AATAAGTTAC TATGGATCAA GTGGATCAGC ACCAGAGGAT 
AAGGATCCTA ACTTGGAATC TATGTTGAAT ATCCCATCAG TACATACTCC AACAGTAATT 
CCTGTTACAG TGAGTATTCC TCAAGGCAAG GGAAAAGGGA AAACAAAAGG CAAAGAAAAA 
CCCAAAGAAT CTCTTAAAGA AGAGGAGCAC CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA 
GAACCAGAGC CTGTTTTACA AGAGAGTCCT CGTGTTCCCA TCTTCCAAAA CCTGAATGTG 
TCTTGCCCCA ATGGGCTCCA AGTGACCTTC ACTGGGCAAG AGTCCCCAGA TCAATATATT 
GTAGGTGAGG AACCCACTTG GGATATCATG GTCCGACAGA ACTACCCCCA GAAGGTGAGG 
CACTATGAGT TCTACAAAAT GGTGATGCCA CCGCTGGAGC AGGAAGCATC GAGAGTTATC 
ACCAGTCAAG GGACTGTTGT CAAATACATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGGACTG TGAGCAGTAG TCCCGATTCA GGTCCTGTCA GTCTTCCTAC TGACGTGCCG 
GCCAGTGCTC ACAGTGCTGA CTTGATGGAC ACAATTTCTC AGCAGAAATC AGAAACAGCA 
CCATCGGAGA CTGTCATCAC AAAGAAAGGA AAAATTCAGA AAAATCAGTC ATCTATGGCA 
CATAAAAATG AAAGCCATGA ACTTCCCCCA GAAACAGTTC AAACAATAAC TCCTATGGAT 
GTTCACTTAG GCACCTGGTT TACAACTACA CCTCAAGGAA ATCGGATTGG CACCAAAGGA 
TCAGAAAGAA TAGCAGACTT GGCCCCATTT TTATCCTTTA AAGCCACAGA TCCTATCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTGATCATCA TTGAAAAGAA AGATGGTACT 
CGGATCGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CCGACTATTT CTTCGGAGGA TCAAGAGACA GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG 
GTGAAGTGCA TGCGGATTGA AAGCTCACAC TACGCCACCA TCATCACCAA CTGCGAGGAC 
AGTAGCTGCT GTGCCACCTT TGCGGACGGG ACGTCCATCA TTGCAAAGCC ACAGGGAACG 
TACCAGGTGT TACCACCAAA CACAGGCTGT CTTCATATTG CTAGGGACTG TTCAGCTATA 
TATTGCCATG AATCAAGCAG TGACATGTAC CATCCTTTTC AAAAACGTGA GCAGCTGAGA 
GCTAGCAGGT ACATCATGAA ACACACATCA GAGGTTATCT GTGAGGTCAT GGATCCTGAA 
GGAAATACCT TTCAGGTCAT GGCTGATGGT AGTGCATCAA CCATCCTACC TGAAAACAAC 
TTGGAAGATG ATTTAAACGC AAAAGCTGAA GGTTATGACA GTTTGTCATC CACTCACCTT 
CATAAGAATC ATCTGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATCTACGCT 
GATGGATCAG GAGTGGAGCT TCTTCGGGAC AGTGACATAG AAGAGTACCT CTCCTTGGCA 
TATGGAGAAT CAACTACCGT AGTTCTTCAA GAGCCAGTAC AGGAACAACC AGGTGCCCTG 
AGCATCACCG TCCTTCGCCC TTTCCATGAA GTGTCGCCGT GGCTAATGAA GAAAGAATTA 
GATACGATTG TTCCTCCTAA TCTCCAGTCA AGGACGTGGG AGACCTTTCC CTCAGTTCAG 
AAAAAAACTC CAGGACCTCC GTTTGGCACT CAGATTTGGA AGGGCCTCGA CATTGGCTCC 
AAACAATTAG TGAACACTCC AGCCCCTATA CTGGAGAGCC CCAATGTGCT ACAGATACGC 
CAGTTCATCC AGCATGAGGT CATAAAGAAT GAGGTGAAAC TGAAACTGCA GCTTTCCCTG 
AAGGACTATA TAAACCATAT TCTCAAGAAA GAAGATGAGT TGCAGGAAAT GACAGTTAAA 
GATTCCAGAA CTGAGGAGGA GAGAGGCAAC GCTGCAGATC TCCTCAAGCT GGTTATGTCT 
TTCCCTAAAA TGGAGGAAAC TACAAAAAGT CATGTTACTG AAGTTGCAGC CCACCTAACT 
GATCTGTTTA AGCAGTCTTT GGCTAATGCT ATGGAATGTC CACCAGAAAC CTTTGAGACA 
GATTTCCTTG AAAAGAAATT GAGGCACACA GAGACGACAA AGCACTGGAA AGAAAAGTTA 
GCTCAAAAGA GGAAGGAAAT TGAAAAAACA CAGAACTATC TAAAGGAAAT TAGGAGCAAA 
GCAATATCAC CCTACTTCAA ATCTGAACTG AGCAAGCTTT ATCAGTCTCA GTTTAATTAT 
CTGGACACCC TTTCCAAAAA CTTGCCTCCT TTTACAAAGA AAGATGAGGA TGCAAGCAAA 
GAAGCTGCTC AAGATACATC CAGTCTTCTT CCAGATTCCC AGAAAAATAA AGTTTCAAAA 
CAGGAATCCT CAGATATACT TAGTGTTTCA AAATCAGAGA ACCTTGAAGA TACCAAGGAG 
CCTGCTAGCC AAAAGCAAAC TGAAAATTTA ACAGTATCTA TCAAAGAACC AGAATCGTGT 
ATGCCTCTGA AAATCCCAAC CCAGTCAATG CTGCAGGATG TGGCAGGACA AGCAAGAAAA 
CAGAAAGTGA GGCTACCTCA CTACTTGCTG AGTTCCAAGC CCAAGTCTCA GCCTCTCACA 
AAGACGCAAG ATTCTGTTGG AGGAAGAGTG AGAACATCCT CTGTGGCATC TGCTGCCATT 
AATAATATAA ATTCATCTCC CTTTGGATTC CATCTTCTCC CACCCGTGGT GAAATTTGGA 
GTGCTTGAAG AAGGACACAC GTATGCCACC ACTGTAAAGC TCAAGAATGT TGGAGTGGAC 
TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA CCCAGCACAG GACTGAAAGT GACTTACAAA 
CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA GACCTGAGAG TGGAGTTATT CGCCATGGCT 
GTCGGGGAGG ATGGCACCAA GGGACTGGCT CACATCTCTC ACAATATAGA GATTATGACT 
GAGCATGACG TCCTGTTCTT ACCTGTGGAA GCAACAGTTT TAACAAGCAG CAATTATGAT 
AAGAGACCAA AAGATTTTCC CCAGGGAAAA GAAAATCCCA CGGTCCAGAG GATTTCTACA 
ATTTCTTCCT CTGCACTCGG AGTCTTCATG TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012034890.1
CDS	218..6091
Translation	MLLLLEIEAEKLKKENGIKELEIFWKYLEPILNNQKPETNLFDVAQLEYTVKADKFPSDWSDSEMLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEGGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLLPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_012179500.1
Organism	Ovis aries(sheep)
Definition	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012179500.1

ORF Insert Sequence:

ATGTTGCTCT TATTAGAAAT AGAAGCTGAA AAACTGAAGA AAGAGAATGG CATAAAAGAA 
CTTGAAATCT TCTGGAAGTA CTTGGAACCA ATCCTGAATA ACCAGAAACC AGAAACAAAT 
CTCTTTGATG TCGCTCAGCT TGAATACACG GTCAAGGCTG ACAAGTTTCC TTCTGACTGG 
TCAGACAGCG AGATGTTGCT GGGATTGGGC ACCGAAATTT TTGAAAATGT TGCCTGTCTG 
ATGTACGACA CCCTGGATTG GAAAAGGCAG CACCAGCACT ATTTGGAAAG CATGCGGCTT 
ATTAACGTCC CACAGGTGGT TAGTGAGAAG TCTGTCTTTG AAGTGACGTT GATTCCTGAG 
CCTCCACAAC CTGTTGTTGC AATCCCTGGA AAGAAGAAAA CGCAACCATA TGAAGAGCCA 
CAGATTCCAG CTCCTCCTCC AGCAACACTG CTACCACCAC CACCAGTGGC TTCCGTCATC 
ACAAATGAAG TAGACATGAG ATACTACAAT GATTTGCTGA ATCCAATCCC AGAAGAATTC 
ATTTCTGTGC CCTTGATACT GCATTGTATG CTGGAACAGG TTGTAGCAAG TGAAGAAGAT 
CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT CCCAGGGCAG ATGGGTTGGA CTACAGAATA 
GCAGCTCACA TCGTATCCAT CTTGCCCTCT CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT 
CTCCATGAAA TATTCTTATC TGAAGAAGAA AATGAAAGCA AACCAGTGCA AACAGGCCCT 
CTCCTGCTTA ACTATCATGA TGCCCACGCC CACAAGAAGT ACATGCTAAA GGACCTAAAG 
AATTTTGATC CAGTTCAAAT TGAACAAGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTC 
CTTAAATTCC CTCTGCCTCC GCCATGGAAT AGCACGAAAC GTCTAGCTAC AATTCATGAG 
CTCATGCACT TTTGTACAAG TGAAGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAA 
GTGTTTACTT TTGAGAGCCT GAAACTCTCT AAGGTTGATG AAGATGGAAA TCTAAAGCAT 
TCTAAAATGA TGTATGGGAC AGATTCTGAG ATGTTCAACA TACCATGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAACAGTAC ATCATGAAAA TGAATACCCA AGAAGCCAAA 
CAGCAAACAG ATGGTGGAAC CAAAGACAGA ATTTTATTTC TGAATCAGAA TTGGTCAACA 
CCTATGCCAG ATGATGAGCT CAACAAAAAA CCATCAGATT ATCGCCAGCC TGGTAGTAAC 
AAGAAGTCCT TAGACTCTGA TAATAGACAA CCAACAGAGC AGGAGACAAA CTTGAAGTCT 
CAACTCCAAC CTGAGACTCT GGAGCAGACC ATGAACAATG AGATCAAAGA TGAAGCGGTC 
ACAAATGCTG ATCTTCTTGA AAAGAAACCA AAGAAGATGA CTGTAGAAGC CGAGTTAGAG 
GACATAAAGA AAACCCAGCA ACGCAATCTA ATGGACTGGA GTTTTACTGA ACATTTTAAA 
CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA GCCAGTCAGC AATACAGGTG TGTTGATTCT 
TATTACCATA CACAAGACAA CTCGTTACTG TTGGTCTTTC ATAATCCAAT GAATTTACAG 
CGTCTGCATC ACGAATATTG GAACGCTGGC CTTCACTCTA ATGTTGGATT CAGAAATTAT 
TTGGAACTTG TTGCAAAATC CATTCAAGAT TGGATCATAA AAGAAGAAGC TATATATCAG 
GAAACTAAAA TGTCTGATGA AATCAATAAA ACTAAGTATG AGCTGGAAAT AAAATCTTCT 
GTTGCTACCA AAGTTTCTTC TACTAGCAAA ATTTATTCTA TTAAGAAATC TAAAAGTAAC 
AAAGGAATGA GCAAAACAGA AATATCAGAT CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA 
AAGATTAATT TTATTTTAGA AGGCTCTCTC AAGGCCTGGA AAGAAGAACA AGATCGGTTA 
GCTGAAGAGG AACGCTTAAA GGAAGAAAAA AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT 
AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA GAAGATAGTA AGATTTTGAA GAAAAAATCT 
TTTATAAAGG AGAAATTGAA AGAAGAGCAA ATGAAGATCC CGGAAGTAAC AGAGGAGCCC 
CTCCAGGAAC CAGAACCTGA GATAGTTTAC CCGTTTCATG GATACAACAT GGGAGAAATA 
CCTGTGCAAA TCTCAGGAAC AAATTATTAT CTGTATCCTT CCGATGGAGG GCAAATTGAA 
GTAGAAAAGA CAACATTTGA AAAAGGTCCA ACAGTTATCA AAATGAAAGT GAAAAAGGAC 
AAGCACAATT TTTTAATTCA CTTAAGAGAC CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA 
GAAGAGGATT ATCCTTCAGA AGATGAGAAA GAAGGAGGAG GAGAGCAAAA CTTCAAAGCA 
GAAGAATCAA ATAAAGAGAA TAAAGCTGTC AGCAAGTTTG GATCTTTTTC TGCCACCTTG 
GAAAATGGAA TCTGCCTCTC AATAAGTTAC TATGGATCAA GTGGATCAGC ACCAGAGGAT 
AAGGATCCTA ACTTGGAATC TATGTTGAAT ATCCCATCAG TACATACTCC AACAGTAATT 
CCTGTTACAG TGAGTATTCC TCAAGGCAAG GGAAAAGGGA AAACAAAAGG CAAAGAAAAA 
CCCAAAGAAT CTCTTAAAGA AGAGGAGCAC CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA 
GAACCAGAGC CTGTTTTACA AGAGAGTCCT CGTGTTCCCA TCTTCCAAAA CCTGAATGTG 
TCTTGCCCCA ATGGGCTCCA AGTGACCTTC ACTGGGCAAG AGTCCCCAGA TCAATATATT 
GTAGGTGAGG AACCCACTTG GGATATCATG GTCCGACAGA ACTACCCCCA GAAGGTGAGG 
CACTATGAGT TCTACAAAAT GGTGATGCCA CCGCTGGAGC AGGAAGCATC GAGAGTTATC 
ACCAGTCAAG GGACTGTTGT CAAATACATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGGACTG TGAGCAGTAG TCCCGATTCA GGTCCTGTCA GTCTTCCTAC TGACGTGCCG 
GCCAGTGCTC ACAGTGCTGA CTTGATGGAC ACAATTTCTC AGCAGAAATC AGAAACAGCA 
CCATCGGAGA CTGTCATCAC AAAGAAAGGA AAAATTCAGA AAAATCAGTC ATCTATGGCA 
CATAAAAATG AAAGCCATGA ACTTCCCCCA GAAACAGTTC AAACAATAAC TCCTATGGAT 
GTTCACTTAG GCACCTGGTT TACAACTACA CCTCAAGGAA ATCGGATTGG CACCAAAGGA 
TCAGAAAGAA TAGCAGACTT GGCCCCATTT TTATCCTTTA AAGCCACAGA TCCTATCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTGATCATCA TTGAAAAGAA AGATGGTACT 
CGGATCGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CCGACTATTT CTTCGGAGGA TCAAGAGACA GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG 
GTGAAGTGCA TGCGGATTGA AAGCTCACAC TACGCCACCA TCATCACCAA CTGCGAGGAC 
AGTAGCTGCT GTGCCACCTT TGCGGACGGG ACGTCCATCA TTGCAAAGCC ACAGGGAACG 
TACCAGGTGT TACCACCAAA CACAGGCTGT CTTCATATTG CTAGGGACTG TTCAGCTATA 
TATTGCCATG AATCAAGCAG TGACATGTAC CATCCTTTTC AAAAACGTGA GCAGCTGAGA 
GCTAGCAGGT ACATCATGAA ACACACATCA GAGGTTATCT GTGAGGTCAT GGATCCTGAA 
GGAAATACCT TTCAGGTCAT GGCTGATGGT AGTGCATCAA CCATCCTACC TGAAAACAAC 
TTGGAAGATG ATTTAAACGC AAAAGCTGAA GGTTATGACA GTTTGTCATC CACTCACCTT 
CATAAGAATC ATCTGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATCTACGCT 
GATGGATCAG GAGTGGAGCT TCTTCGGGAC AGTGACATAG AAGAGTACCT CTCCTTGGCA 
TATGGAGAAT CAACTACCGT AGTTCTTCAA GAGCCAGTAC AGGAACAACC AGGTGCCCTG 
AGCATCACCG TCCTTCGCCC TTTCCATGAA GTGTCGCCGT GGCTAATGAA GAAAGAATTA 
GATACGATTG TTCCTCCTAA TCTCCAGTCA AGGACGTGGG AGACCTTTCC CTCAGTTCAG 
AAAAAAACTC CAGGACCTCC GTTTGGCACT CAGATTTGGA AGGGCCTCGA CATTGGCTCC 
AAACAATTAG TGAACACTCC AGCCCCTATA CTGGAGAGCC CCAATGTGCT ACAGATACGC 
CAGTTCATCC AGCATGAGGT CATAAAGAAT GAGGTGAAAC TGAAACTGCA GCTTTCCCTG 
AAGGACTATA TAAACCATAT TCTCAAGAAA GAAGATGAGT TGCAGGAAAT GACAGTTAAA 
GATTCCAGAA CTGAGGAGGA GAGAGGCAAC GCTGCAGATC TCCTCAAGCT GGTTATGTCT 
TTCCCTAAAA TGGAGGAAAC TACAAAAAGT CATGTTACTG AAGTTGCAGC CCACCTAACT 
GATCTGTTTA AGCAGTCTTT GGCTAATGCT ATGGAATGTC CACCAGAAAC CTTTGAGACA 
GATTTCCTTG AAAAGAAATT GAGGCACACA GAGACGACAA AGCACTGGAA AGAAAAGTTA 
GCTCAAAAGA GGAAGGAAAT TGAAAAAACA CAGAACTATC TAAAGGAAAT TAGGAGCAAA 
GCAATATCAC CCTACTTCAA ATCTGAACTG AGCAAGCTTT ATCAGTCTCA GTTTAATTAT 
CTGGACACCC TTTCCAAAAA CTTGCCTCCT TTTACAAAGA AAGATGAGGA TGCAAGCAAA 
GAAGCTGCTC AAGATACATC CAGTCTTCTT CCAGATTCCC AGAAAAATAA AGTTTCAAAA 
CAGGAATCCT CAGATATACT TAGTGTTTCA AAATCAGAGA ACCTTGAAGA TACCAAGGAG 
CCTGCTAGCC AAAAGCAAAC TGAAAATTTA ACAGTATCTA TCAAAGAACC AGAATCGTGT 
ATGCCTCTGA AAATCCCAAC CCAGTCAATG CTGCAGGATG TGGCAGGACA AGCAAGAAAA 
CAGAAAGTGA GGCTACCTCA CTACTTGCTG AGTTCCAAGC CCAAGTCTCA GCCTCTCACA 
AAGACGCAAG ATTCTGTTGG AGGAAGAGTG AGAACATCCT CTGTGGCATC TGCTGCCATT 
AATAATATAA ATTCATCTCC CTTTGGATTC CATCTTCTCC CACCCGTGGT GAAATTTGGA 
GTGCTTGAAG AAGGACACAC GTATGCCACC ACTGTAAAGC TCAAGAATGT TGGAGTGGAC 
TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA CCCAGCACAG GACTGAAAGT GACTTACAAA 
CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA GACCTGAGAG TGGAGTTATT CGCCATGGCT 
GTCGGGGAGG ATGGCACCAA GGGACTGGCT CACATCTCTC ACAATATAGA GATTATGACT 
GAGCATGACG TCCTGTTCTT ACCTGTGGAA GCAACAGTTT TAACAAGCAG CAATTATGAT 
AAGAGACCAA AAGATTTTCC CCAGGGAAAA GAAAATCCCA CGGTCCAGAG GATTTCTACA 
ATTTCTTCCT CTGCACTCGG AGTCTTCATG TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_027973609.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_027829410.1 Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm57766
Clone ID Related Accession (Same CDS sequence)	XM_027973609.1
Accession Version	XM_027973609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	sperm-associated antigen 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGG GATTGGGCAC CGAAATTTTT GAAAATGTTG CCTGTCTGAT GTACGACACC 
CTGGATTGGA AAAGGCAGCA CCAGCACTAT TTGGAAAGCA TGCGGCTTAT TAACGTCCCA 
CAGGTGGTTA GTGAGAAGTC TGTCTTTGAA GTGACGTTGA TTCCTGAGCC TCCACAACCT 
GTTGTTGCAA TCCCTGGAAA GAAGAAAACA CAACCATATG AAGAGCCACA GATTCCAGCT 
CCTCCTCCAG CAACACTGCT ACCACCACCA CCAGTGGCTT CCATCATCAC AAATGAAGTA 
GACATGAGAT ACTACAATGA TTTGCTGAAT CCAATCCCAG AAGAATTCAT TTCTGTGCCC 
TTGATACTGC ATTGTATGCT GGAACAGGTT GTAGCAAGTG AAGAAGATCT TGTCCCACCA 
AGTCTTGTAG AGCTGCCTCC CAGGGCAGAT GGGTTGGACT ACAGAATAGC AGCTCACATC 
GTATCCATCT TGCCCTCTCT CTGTCTGACA GAGAGGGAGA AAAAGAATCT CCATGAAATA 
TTCTTATCTG AAGAAGAAAA TGAAAGCAAA CCAGTGCAAA CAGGCCCTCT CCTGCTTAAC 
TATCATGATG CCCACGCCCA CAAGAAGTAC ATGCTAAAGG ACCTAAAGAA TTTTGATCCA 
GTTCAAATTG AACAAGAGAT GCAGTCCAAG TTGCCACTGT GGGAATTCCT TAAATTCCCT 
CTACCTCCGC CATGGAATAG CACGAAACGT CTAGCTACAA TTCATGAGCT CATGCACTTT 
TGTACAAGTG AAGTCTTGAG CTGGAATGAA GTAGAACGAG CCTTCAAAGT GTTTACTTTT 
GAGAGCCTGA AACTCTCTAA GGTTGATGAA GATGGAAATC TAAAGCATTC TAAAATGATG 
TATGGGACAG ATTCTGAGAT GTTCAACATA CCATGGGACA ACCCTGCCAG ATTTGCTAAA 
CAGATAAGGC AACAGTACAT CATGAAAATG AATACCCAAG AAGCCAAACA GCAAACAGAT 
GGTGGAACCA AAGACAGAAT TTTATTTCTG AATCAGAATT GGTCAACACC TATGCCAGAT 
GATGAGCTCA ACAAAAAACC ATCAGATTAT CGCCAGCCTG GTAGTAACAA GAAGTCCTTA 
GACTCTGATA ATAGACAACC AACAGAGCAG GAGACAAACT TGAAGTCTCA GCTCCAACCT 
GAGACTCTGG AGCAGACCAT GAACAATGAG ATCAAAGATG AAGCGGTCAC AAATGCTGAT 
CTTCTTGAAA AGAAACCAAA GAAGATGACT GTAGAAGCCG AGTTAGAGGA CATAAAGAAA 
ACCCAGCAAC GCAATCTAAT GGACTGGAGT TTTACTGAAC ATTTTAAACC AAAAGTTCTG 
CTTCAGGTTC TTCAAGAAGC CAGTCAGCAA TACAGGTGTG TTGATTCTTA TTACCATACA 
CAAGACAACT CGTTACTGTT GGTCTTTCAT AATCCAATGA ATTTACAGCG TCTGCATCAC 
GAATATTGGA ACGCTGGCCT TCACTCTAAT GTTGGATTCA GAAATTATTT GGAACTTGTT 
GCAAAATCCA TTCAAGATTG GATCATAAAA GAAGAAGCTA TATATCAGGA AACTAAAATG 
TCTGATGAAA TCAATAAAAC TAAGTATGAG CTGGAAATAA AATCTTCTGT TGCTACCAAA 
GTTTCTTCTA CTAGCAAAAT TTATTCTATT AAGAAATCTA AAAGTAACAA AGGAATGAGC 
AAAACAGAAA TATCAGATCA AGAAAAAGAC AAAGAAAAAG AGAAGGAAAA GATTAATTTT 
ATTTTAGAAG GCTCTCTCAA GGCCTGGAAA GAAGAACAAG ATCGGTTAGC TGAAGAGGAA 
CGCTTAAAGG AAGAAAAAAA AGCAGAAAAG AAGAGTAAGG AGGCTGGTAA AAAGAAAGGC 
AAGGAAAAGC TAGAAAAAGA AGATAGTAAG ATTTTGAAGA AAAAATCTTT TATAAAGGAG 
AAATTGAAAG AAGAGCAAAT GAAGATCCCG GAAGTAACAG AGGAGCCCCT CCAGGAACCA 
GAACCTGAGA TAGTTTACCC GTTTCATGGA TACAACATGG GAGAAATACC TGTGCAAATC 
TCAGGAACAA ATTATTATCT GTATCCTTCC GATGGAGGGC AAATTGAAGT AGAAAAGACA 
ACATTTGAAA AAGGTCCAAC AGTTATCAAA ATGAAAGTGA AAAAGGACAA GCACAATTTT 
TTAATTCACT TAAGAGACCC TAGGAAAATT GTGGAAAAGG AGAAAAAAGA AGAGGATTAT 
CCTTCAGAAG ATGAGAAAGA AGAAGGAGGA GAGCAAAACT TCAAAGCAGA AGAATCAAAT 
AAAGAGAATA AAGCTGTCAG CAAGTTTGGA TCTTTTTCTG CCACCTTGGA AAATGGAATC 
TGCCTCTCAA TAAGTTACTA TGGATCAAGT GGATCAGCAC CAGAGGATAA GGATCCTAAC 
TTGGAATCTA TGTTGAATAT CCCATCAGTA CATACTCCAA CAGTAATTCC TGTTACAGTG 
AGTATTCCTC AAGGCAAGGG AAAAGGGAAA ACAAAAGGCA AAGAAAAACC CAAAGAATCT 
CTTAAAGAAG AGGAGCACCC AAAAGAAGAA GAGAAAAAGG AAGTAGAAGA ACCAGAGCCT 
GTTTTACAAG AGAGTCCTCG TGTTCCCATC TTCCAAAACC TGAATGTGTC TTGCCCCAAT 
GGGCTCCAAG TGACCTTCAC TGGGCAAGAG TCCCCAGATC AATATATTGT AGGTGAGGAA 
CCCACTTGGG ATATCATGGT CCGACAGAAC TACCCCCAGA AGGTGAGGCA CTATGAGTTC 
TACAAAATGG TGATGCCACC GCTGGAGCAG GAAGCATCGA GAGTTATCAC CAGTCAAGGG 
ACTGTTGTCA AATACATGCT GGATGGATCC ACACAGATTC TCTTTGCAGA TGGGACTGTG 
AGCAGTAGTC CCGATTCAGG TCCTGTCAGT CTTCCTACTG ACGTGCCGGC CAGTGCTCAC 
AGTGCTGACT TGATGGACAC AATTTCTCAG CAGAAATCAG AAACAGCACC ATCGGAGACT 
GTCATCACAA AGAAAGGAAA AATTCAGAAA AATCAGTCAT CTATGGCACA TAAAAATGAA 
AGCCATGAAC TTCCCCCAGA AACAGTTCAA ACAATAACTC CTATGGATGT TCACTTAGGC 
ACCTGGTTTA CAACTACACC TCAAGGAAAT CGGATTGGCA CCAAAGGATC AGAAAGAATA 
GCAGACTTGG CCCCATTTTT ATCCTTTAAA GCCACAGATC CTATCAATGG AACGGTTATG 
ACAACTCGAG AAGACAAAGT GATCATCATT GAAAAGAAAG ATGGTACTCG GATCGTGGAT 
CATGCTGATG GTACCAGAAT CACAACCTTT TATCAAGTTT ATGAAGATCC GACTATTTCT 
TCGGAGGATC AAGAGACAGC CGAAGGTCCT CAGAGGATCG CCAGGCAGGT GAAGTGCATG 
CGGATTGAAA GCTCACACTA CGCCACCATC ATCACCAACT GCGAGGACAG TAGCTGCTGT 
GCCACCTTTG CGGACGGGAC GTCCATCATT GCAAAGCCAC AGGGAACGTA CCAGGTGTTA 
CCACCAAACA CAGGCTGTCT TCATATTGCT AGGGACTGTT CAGCTATATA TTGCCATGAA 
TCAAGCAGTG ACATGTACCA TCCTTTTCAA AAACGTGAGC AGCTGAGAGC TAGCAGGTAC 
ATCATGAAAC ACACATCAGA GGTTATCTGC GAGGTCATGG ATCCTGAAGG AAATACCTTT 
CAGGTCATGG CTGATGGTAG TGCATCAACC ATCCTACCTG AAAACAACTT GGAAGATGAT 
TTAAACGCAA AAGCTGAAGG TTATGACAGT TTGTCATCCA CTCACCTTCA TAAGAATCAT 
CTGCAAATCT ATGGTGAACA TGTCCCCAGG TTTTTTGTTA TCTATGCTGA TGGATCAGGA 
GTGGAGCTTC TTCGGGACAG TGACATAGAA GAGTACCTCT CCTTGGCATA TGGAGAATCA 
ACTACCGTAG TTCTTCAAGA GCCAGTACAG GAACAACCAG GTGCCCTGAG CATCACCGTC 
CTTCGCCCTT TCCATGAAGT GTCGCCGTGG CTAATGAAGA AAGAATTAGA TACGATTGTT 
CCTCCTAATC TCCAGTCAAG GACGTGGGAG ACCTTTCCCT CAGTTCAGAA AAAAACTCCA 
GGACCTCCGT TTGGCACTCA GATTTGGAAG GGCCTCGACA TTGGCTCCAA ACAATTAGTG 
AACACTCCAG CCCCTATACT GGAGAGCCCC AATGTGCTAC AGATACGCCA GTTCATCCAG 
CATGAGGTCA TAAAGAATGA GGTGAAACTG AAACTGCAGC TTTCCCTGAA GGACTATATA 
AACCATATTC TCAAGAAAGA AGATGAGTTG CAGGAAATGA CAGTTAAAGA TTCCAGAACT 
GAGGAGGAGA GAGGCAACGC TGCAGATCTC CTCAAGCTGG TTATGTCTTT CCCTAAAATG 
GAGGAAACTA CAAAAAGTCA TGTTACTGAA GTTGCAGCCC ACCTAACTGA TCTGTTTAAG 
CAGTCTTTGG CTAATGCTAT GGAATGTCCA CCAGAAACCT TTGAGACAGA TTTCCTTGAA 
AAGAAATTGA GGCACACAGA GACGACAAAG CACTGGAAAG AAAAGTTAGC TCAAAAGAGG 
AAGGAAATTG AAAAAACACA GAACTATCTA AAGGAAATTA GGAGCAAAGC AATATCACCC 
TACTTCAAAT CTGAACTGAG CAAGCTTTAT CAGTCTCAGT TTAATTATCT GGACACCCTT 
TCCAAAAACT TGCCTCCTTT TACAAAGAAA GATGAGGATG CAAGCAAAGA AGCCGCTCAA 
GATACATCCA GTCTTCCTCC AGATTCCCAG AAAAATAAAG TTTCAAAACA GGAATCCTCA 
GATATACTTA GTGTTTCAAA ATCAGAGAAC CTTGAAGATA CCAAGGAGCC TGCTAGCCAA 
AAGCAAACTG AAAATTTAAC AGTATCTATC AAAGAACCAG AATCGTGTAT GCCTCTGAAA 
ATCCCAACCC AGTCAATGCT GCAGGATGTG GCAGGACAAG CAAGAAAACA GAAAGTGAGG 
CTGCCTCACT ACTTGCTGAG TTCCAAGCCC AAGTCTCAGC CTCTCACAAA GACGCAAGAT 
TCTGTGGGAG GAAGAGTGAG AACATCCTCT GTGGCATCTG CTGCCATTAA TAATATAAAT 
TCATCTCCCT TTGGATTCCA TCTTCTCCCA CCCGTGGTGA AATTTGGAGT GCTCGAAGAA 
GGACACACGT ATGCCACCAC TGTAAAGCTC AAGAATGTTG GAGTGGACTT CTGCAGGTTT 
AGGGTGAAGC AGCCTCCACC CAGCACAGGA CTGAAAGTGA CTTACAAACC TGGGCCTGTG 
GCGGCTGGTT TGCAGGCAGA CCTGAGAGTG GAGTTATTCG CCATGGCTGT CGGGGAGGAT 
GGCACCAAGG GACTGGCTCA CATCTCTCAC AATATAGAGA TTATGACTGA GCATGACGTC 
CTGTTCTTAC CTGTGGAAGC AACAGTTTTA ACAAGCAGCA ATTATGATAA GAGACCAAAA 
GATTTTCCCC AGGGAAAAGA AAATCCCACG GTCCAGAGGA TTTCTACAAT TTCTTCCTCT 
GCACTCGGAG TCTTCATGTC CCGTAAAATT TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027829410.1
CDS	39..5720
Translation	MLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASIITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEEGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLPPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_027973609.1
Organism	Ovis aries(sheep)
Definition	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027973609.1

ORF Insert Sequence:

ATGTTGCTGG GATTGGGCAC CGAAATTTTT GAAAATGTTG CCTGTCTGAT GTACGACACC 
CTGGATTGGA AAAGGCAGCA CCAGCACTAT TTGGAAAGCA TGCGGCTTAT TAACGTCCCA 
CAGGTGGTTA GTGAGAAGTC TGTCTTTGAA GTGACGTTGA TTCCTGAGCC TCCACAACCT 
GTTGTTGCAA TCCCTGGAAA GAAGAAAACA CAACCATATG AAGAGCCACA GATTCCAGCT 
CCTCCTCCAG CAACACTGCT ACCACCACCA CCAGTGGCTT CCATCATCAC AAATGAAGTA 
GACATGAGAT ACTACAATGA TTTGCTGAAT CCAATCCCAG AAGAATTCAT TTCTGTGCCC 
TTGATACTGC ATTGTATGCT GGAACAGGTT GTAGCAAGTG AAGAAGATCT TGTCCCACCA 
AGTCTTGTAG AGCTGCCTCC CAGGGCAGAT GGGTTGGACT ACAGAATAGC AGCTCACATC 
GTATCCATCT TGCCCTCTCT CTGTCTGACA GAGAGGGAGA AAAAGAATCT CCATGAAATA 
TTCTTATCTG AAGAAGAAAA TGAAAGCAAA CCAGTGCAAA CAGGCCCTCT CCTGCTTAAC 
TATCATGATG CCCACGCCCA CAAGAAGTAC ATGCTAAAGG ACCTAAAGAA TTTTGATCCA 
GTTCAAATTG AACAAGAGAT GCAGTCCAAG TTGCCACTGT GGGAATTCCT TAAATTCCCT 
CTACCTCCGC CATGGAATAG CACGAAACGT CTAGCTACAA TTCATGAGCT CATGCACTTT 
TGTACAAGTG AAGTCTTGAG CTGGAATGAA GTAGAACGAG CCTTCAAAGT GTTTACTTTT 
GAGAGCCTGA AACTCTCTAA GGTTGATGAA GATGGAAATC TAAAGCATTC TAAAATGATG 
TATGGGACAG ATTCTGAGAT GTTCAACATA CCATGGGACA ACCCTGCCAG ATTTGCTAAA 
CAGATAAGGC AACAGTACAT CATGAAAATG AATACCCAAG AAGCCAAACA GCAAACAGAT 
GGTGGAACCA AAGACAGAAT TTTATTTCTG AATCAGAATT GGTCAACACC TATGCCAGAT 
GATGAGCTCA ACAAAAAACC ATCAGATTAT CGCCAGCCTG GTAGTAACAA GAAGTCCTTA 
GACTCTGATA ATAGACAACC AACAGAGCAG GAGACAAACT TGAAGTCTCA GCTCCAACCT 
GAGACTCTGG AGCAGACCAT GAACAATGAG ATCAAAGATG AAGCGGTCAC AAATGCTGAT 
CTTCTTGAAA AGAAACCAAA GAAGATGACT GTAGAAGCCG AGTTAGAGGA CATAAAGAAA 
ACCCAGCAAC GCAATCTAAT GGACTGGAGT TTTACTGAAC ATTTTAAACC AAAAGTTCTG 
CTTCAGGTTC TTCAAGAAGC CAGTCAGCAA TACAGGTGTG TTGATTCTTA TTACCATACA 
CAAGACAACT CGTTACTGTT GGTCTTTCAT AATCCAATGA ATTTACAGCG TCTGCATCAC 
GAATATTGGA ACGCTGGCCT TCACTCTAAT GTTGGATTCA GAAATTATTT GGAACTTGTT 
GCAAAATCCA TTCAAGATTG GATCATAAAA GAAGAAGCTA TATATCAGGA AACTAAAATG 
TCTGATGAAA TCAATAAAAC TAAGTATGAG CTGGAAATAA AATCTTCTGT TGCTACCAAA 
GTTTCTTCTA CTAGCAAAAT TTATTCTATT AAGAAATCTA AAAGTAACAA AGGAATGAGC 
AAAACAGAAA TATCAGATCA AGAAAAAGAC AAAGAAAAAG AGAAGGAAAA GATTAATTTT 
ATTTTAGAAG GCTCTCTCAA GGCCTGGAAA GAAGAACAAG ATCGGTTAGC TGAAGAGGAA 
CGCTTAAAGG AAGAAAAAAA AGCAGAAAAG AAGAGTAAGG AGGCTGGTAA AAAGAAAGGC 
AAGGAAAAGC TAGAAAAAGA AGATAGTAAG ATTTTGAAGA AAAAATCTTT TATAAAGGAG 
AAATTGAAAG AAGAGCAAAT GAAGATCCCG GAAGTAACAG AGGAGCCCCT CCAGGAACCA 
GAACCTGAGA TAGTTTACCC GTTTCATGGA TACAACATGG GAGAAATACC TGTGCAAATC 
TCAGGAACAA ATTATTATCT GTATCCTTCC GATGGAGGGC AAATTGAAGT AGAAAAGACA 
ACATTTGAAA AAGGTCCAAC AGTTATCAAA ATGAAAGTGA AAAAGGACAA GCACAATTTT 
TTAATTCACT TAAGAGACCC TAGGAAAATT GTGGAAAAGG AGAAAAAAGA AGAGGATTAT 
CCTTCAGAAG ATGAGAAAGA AGAAGGAGGA GAGCAAAACT TCAAAGCAGA AGAATCAAAT 
AAAGAGAATA AAGCTGTCAG CAAGTTTGGA TCTTTTTCTG CCACCTTGGA AAATGGAATC 
TGCCTCTCAA TAAGTTACTA TGGATCAAGT GGATCAGCAC CAGAGGATAA GGATCCTAAC 
TTGGAATCTA TGTTGAATAT CCCATCAGTA CATACTCCAA CAGTAATTCC TGTTACAGTG 
AGTATTCCTC AAGGCAAGGG AAAAGGGAAA ACAAAAGGCA AAGAAAAACC CAAAGAATCT 
CTTAAAGAAG AGGAGCACCC AAAAGAAGAA GAGAAAAAGG AAGTAGAAGA ACCAGAGCCT 
GTTTTACAAG AGAGTCCTCG TGTTCCCATC TTCCAAAACC TGAATGTGTC TTGCCCCAAT 
GGGCTCCAAG TGACCTTCAC TGGGCAAGAG TCCCCAGATC AATATATTGT AGGTGAGGAA 
CCCACTTGGG ATATCATGGT CCGACAGAAC TACCCCCAGA AGGTGAGGCA CTATGAGTTC 
TACAAAATGG TGATGCCACC GCTGGAGCAG GAAGCATCGA GAGTTATCAC CAGTCAAGGG 
ACTGTTGTCA AATACATGCT GGATGGATCC ACACAGATTC TCTTTGCAGA TGGGACTGTG 
AGCAGTAGTC CCGATTCAGG TCCTGTCAGT CTTCCTACTG ACGTGCCGGC CAGTGCTCAC 
AGTGCTGACT TGATGGACAC AATTTCTCAG CAGAAATCAG AAACAGCACC ATCGGAGACT 
GTCATCACAA AGAAAGGAAA AATTCAGAAA AATCAGTCAT CTATGGCACA TAAAAATGAA 
AGCCATGAAC TTCCCCCAGA AACAGTTCAA ACAATAACTC CTATGGATGT TCACTTAGGC 
ACCTGGTTTA CAACTACACC TCAAGGAAAT CGGATTGGCA CCAAAGGATC AGAAAGAATA 
GCAGACTTGG CCCCATTTTT ATCCTTTAAA GCCACAGATC CTATCAATGG AACGGTTATG 
ACAACTCGAG AAGACAAAGT GATCATCATT GAAAAGAAAG ATGGTACTCG GATCGTGGAT 
CATGCTGATG GTACCAGAAT CACAACCTTT TATCAAGTTT ATGAAGATCC GACTATTTCT 
TCGGAGGATC AAGAGACAGC CGAAGGTCCT CAGAGGATCG CCAGGCAGGT GAAGTGCATG 
CGGATTGAAA GCTCACACTA CGCCACCATC ATCACCAACT GCGAGGACAG TAGCTGCTGT 
GCCACCTTTG CGGACGGGAC GTCCATCATT GCAAAGCCAC AGGGAACGTA CCAGGTGTTA 
CCACCAAACA CAGGCTGTCT TCATATTGCT AGGGACTGTT CAGCTATATA TTGCCATGAA 
TCAAGCAGTG ACATGTACCA TCCTTTTCAA AAACGTGAGC AGCTGAGAGC TAGCAGGTAC 
ATCATGAAAC ACACATCAGA GGTTATCTGC GAGGTCATGG ATCCTGAAGG AAATACCTTT 
CAGGTCATGG CTGATGGTAG TGCATCAACC ATCCTACCTG AAAACAACTT GGAAGATGAT 
TTAAACGCAA AAGCTGAAGG TTATGACAGT TTGTCATCCA CTCACCTTCA TAAGAATCAT 
CTGCAAATCT ATGGTGAACA TGTCCCCAGG TTTTTTGTTA TCTATGCTGA TGGATCAGGA 
GTGGAGCTTC TTCGGGACAG TGACATAGAA GAGTACCTCT CCTTGGCATA TGGAGAATCA 
ACTACCGTAG TTCTTCAAGA GCCAGTACAG GAACAACCAG GTGCCCTGAG CATCACCGTC 
CTTCGCCCTT TCCATGAAGT GTCGCCGTGG CTAATGAAGA AAGAATTAGA TACGATTGTT 
CCTCCTAATC TCCAGTCAAG GACGTGGGAG ACCTTTCCCT CAGTTCAGAA AAAAACTCCA 
GGACCTCCGT TTGGCACTCA GATTTGGAAG GGCCTCGACA TTGGCTCCAA ACAATTAGTG 
AACACTCCAG CCCCTATACT GGAGAGCCCC AATGTGCTAC AGATACGCCA GTTCATCCAG 
CATGAGGTCA TAAAGAATGA GGTGAAACTG AAACTGCAGC TTTCCCTGAA GGACTATATA 
AACCATATTC TCAAGAAAGA AGATGAGTTG CAGGAAATGA CAGTTAAAGA TTCCAGAACT 
GAGGAGGAGA GAGGCAACGC TGCAGATCTC CTCAAGCTGG TTATGTCTTT CCCTAAAATG 
GAGGAAACTA CAAAAAGTCA TGTTACTGAA GTTGCAGCCC ACCTAACTGA TCTGTTTAAG 
CAGTCTTTGG CTAATGCTAT GGAATGTCCA CCAGAAACCT TTGAGACAGA TTTCCTTGAA 
AAGAAATTGA GGCACACAGA GACGACAAAG CACTGGAAAG AAAAGTTAGC TCAAAAGAGG 
AAGGAAATTG AAAAAACACA GAACTATCTA AAGGAAATTA GGAGCAAAGC AATATCACCC 
TACTTCAAAT CTGAACTGAG CAAGCTTTAT CAGTCTCAGT TTAATTATCT GGACACCCTT 
TCCAAAAACT TGCCTCCTTT TACAAAGAAA GATGAGGATG CAAGCAAAGA AGCCGCTCAA 
GATACATCCA GTCTTCCTCC AGATTCCCAG AAAAATAAAG TTTCAAAACA GGAATCCTCA 
GATATACTTA GTGTTTCAAA ATCAGAGAAC CTTGAAGATA CCAAGGAGCC TGCTAGCCAA 
AAGCAAACTG AAAATTTAAC AGTATCTATC AAAGAACCAG AATCGTGTAT GCCTCTGAAA 
ATCCCAACCC AGTCAATGCT GCAGGATGTG GCAGGACAAG CAAGAAAACA GAAAGTGAGG 
CTGCCTCACT ACTTGCTGAG TTCCAAGCCC AAGTCTCAGC CTCTCACAAA GACGCAAGAT 
TCTGTGGGAG GAAGAGTGAG AACATCCTCT GTGGCATCTG CTGCCATTAA TAATATAAAT 
TCATCTCCCT TTGGATTCCA TCTTCTCCCA CCCGTGGTGA AATTTGGAGT GCTCGAAGAA 
GGACACACGT ATGCCACCAC TGTAAAGCTC AAGAATGTTG GAGTGGACTT CTGCAGGTTT 
AGGGTGAAGC AGCCTCCACC CAGCACAGGA CTGAAAGTGA CTTACAAACC TGGGCCTGTG 
GCGGCTGGTT TGCAGGCAGA CCTGAGAGTG GAGTTATTCG CCATGGCTGT CGGGGAGGAT 
GGCACCAAGG GACTGGCTCA CATCTCTCAC AATATAGAGA TTATGACTGA GCATGACGTC 
CTGTTCTTAC CTGTGGAAGC AACAGTTTTA ACAAGCAGCA ATTATGATAA GAGACCAAAA 
GATTTTCCCC AGGGAAAAGA AAATCCCACG GTCCAGAGGA TTTCTACAAT TTCTTCCTCT 
GCACTCGGAG TCTTCATGTC CCGTAAAATT TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_012146169.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012001559.1 Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

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CloneID	OOv23605
Clone ID Related Accession (Same CDS sequence)	XM_012146169.2
Accession Version	XM_012146169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	sperm-associated antigen 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942555.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012146169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AGGCGGGACA ACCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTCATA GAAGACAGAT TTAAGATAGA AAAGGATAAA 
GTGAAAGCAA AATCTCCCAA GGAGAAGAAG TCCCCATCTG CCAAGGTTGC CAAAGGAAAG 
GGAAAGGACC AGACAGAGGC AAACACTGCC GTGAAAAAGA CCACCCAGTT AGTGAGGAGG 
GGGGAAGAGG ATGACATCAA ACAATACATT GACGATGAGC CAGATGACGG TGCCCAGCAT 
TACTTTATAG TCGTGGGCTT CAACAATCCT CAGCTATTAG CGATCATGAC GGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGACACAC 
CTGGCAGCAG TTAGCCAACA GCAGGAAAGT TTTCTTCAGC CAGAAGAAAT AGAAGCTGAA 
AAACTGAAGA AAGAGAATGG CATAAAAGAA CTTGAAATCT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ACCAGAAACC AGAAACAAAT CTCTTTGATG TCGCTCAGCT TGAATACACG 
GTCAAGGCTG ACAAGTTTCC TTCTGACTGG TCAGACAGCG AGATGTTGCT GGGATTGGGC 
ACCGAAATTT TTGAAAATGT TGCCTGTCTG ATGTACGACA CCCTGGATTG GAAAAGGCAG 
CACCAGCACT ATTTGGAAAG CATGCGGCTT ATTAACGTCC CACAGGTGGT TAGTGAGAAG 
TCTGTCTTTG AAGTGACGTT GATTCCTGAG CCTCCACAAC CTGTTGTTGC AATCCCTGGA 
AAGAAGAAAA CGCAACCATA TGAAGAGCCA CAGATTCCAG CTCCTCCTCC AGCAACACTG 
CTACCACCAC CACCAGTGGC TTCCGTCATC ACAAATGAAG TAGACATGAG ATACTACAAT 
GATTTGCTGA ATCCAATCCC AGAAGAATTC ATTTCTGTGC CCTTGATACT GCATTGTATG 
CTGGAACAGG TTGTAGCAAG TGAAGAAGAT CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT 
CCCAGGGCAG ATGGGTTGGA CTACAGAATA GCAGCTCACA TCGTATCCAT CTTGCCCTCT 
CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT CTCCATGAAA TATTCTTATC TGAAGAAGAA 
AATGAAAGCA AACCAGTGCA AACAGGCCCT CTCCTGCTTA ACTATCATGA TGCCCACGCC 
CACAAGAAGT ACATGCTAAA GGACCTAAAG AATTTTGATC CAGTTCAAAT TGAACAAGAG 
ATGCAGTCCA AGTTGCCACT GTGGGAATTC CTTAAATTCC CTCTACCTCC GCCATGGAAT 
AGCACGAAAC GTCTAGCTAC AATTCATGAG CTCATGCACT TTTGTACAAG TGAAGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAA GTGTTTACTT TTGAGAGCCT GAAACTCTCT 
AAGGTTGATG AAGATGGAAA TCTAAAGCAT TCTAAAATGA TGTATGGGAC AGATTCTGAG 
ATGTTCAACA TACCATGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAACAGTAC 
ATCATGAAAA TGAATACCCA AGAAGCCAAA CAGCAAACAG ATGGTGGAAC CAAAGACAGA 
ATTTTATTTC TGAATCAGAA TTGGTCAACA CCTATGCCAG ATGATGAGCT CAACAAAAAA 
CCATCAGATT ATCGCCAGCC TGGTAGTAAC AAGAAGTCCT TAGACTCTGA TAATAGACAA 
CCAACAGAGC AGGAGACAAA CTTGAAGTCT CAGCTCCAAC CTGAGACTCT GGAGCAGACC 
ATGAACAATG AGATCAAAGA TGAAGCGGTC ACAAATGCTG ATCTTCTTGA AAAGAAACCA 
AAGAAGATGA CTGTAGAAGC CGAGTTAGAG GACATAAAGA AAACCCAGCA ACGCAATCTA 
ATGGACTGGA GTTTTACTGA ACATTTTAAA CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA 
GCCAGTCAGC AATACAGGTG TGTTGATTCT TATTACCATA CACAAGACAA CTCGTTACTG 
TTGGTCTTTC ATAATCCAAT GAATTTACAG CGTCTGCATC ACGAATATTG GAACGCTGGC 
CTTCACTCTA ATGTTGGATT CAGAAATTAT TTGGAACTTG TTGCAAAATC CATTCAAGAT 
TGGATCATAA AAGAAGAAGC TATATATCAG GAAACTAAAA TGTCTGATGA AATCAATAAA 
ACTAAGTATG AGCTGGAAAT AAAATCTTCT GTTGCTACCA AAGTTTCTTC TACTAGCAAA 
ATTTATTCTA TTAAGAAATC TAAAAGTAAC AAAGGAATGA GCAAAACAGA AATATCAGAT 
CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA AAGATTAATT TTATTTTAGA AGGCTCTCTC 
AAGGCCTGGA AAGAAGAACA AGATCGGTTA GCTGAAGAGG AACGCTTAAA GGAAGAAAAA 
AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA 
GAAGATAGTA AGATTTTGAA GAAAAAATCT TTTATAAAGG AGAAATTGAA AGAAGAGCAA 
ATGAAGATCC CGGAAGTAAC AGAGGAGCCC CTCCAGGAAC CAGAACCTGA GATAGTTTAC 
CCGTTTCATG GATACAACAT GGGAGAAATA CCTGTGCAAA TCTCAGGAAC AAATTATTAT 
CTGTATCCTT CCGATGGAGG GCAAATTGAA GTAGAAAAGA CAACATTTGA AAAAGGTCCA 
ACAGTTATCA AAATGAAAGT GAAAAAGGAC AAGCACAATT TTTTAATTCA CTTAAGAGAC 
CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA GAAGAGGATT ATCCTTCAGA AGATGAGAAA 
GAAGAAGGAG GAGAGCAAAA CTTCAAAGCA GAAGAATCAA ATAAAGAGAA TAAAGCTGTC 
AGCAAGTTTG GATCTTTTTC TGCCACCTTG GAAAATGGAA TCTGCCTCTC AATAAGTTAC 
TATGGATCAA GTGGATCAGC ACCAGAGGAT AAGGATCCTA ACTTGGAATC TATGTTGAAT 
ATCCCATCAG TACATACTCC AACAGTAATT CCTGTTACAG TGAGTATTCC TCAAGGCAAG 
GGAAAAGGGA AAACAAAAGG CAAAGAAAAA CCCAAAGAAT CTCTTAAAGA AGAGGAACAC 
CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA GAACCAGAGC CTGTTTTACA AGAGAGTCCT 
CGTGTTCCCA TCTTCCAAAA CCTGAATGTG TCTTGCCCCA ATGGGCTCCA AGTGACCTTC 
ACTGGGCAAG AGTCCCCAGA TCAATATATT GTAGGTGAGG AACCCACTTG GGATATCATG 
GTCCGACAGA ACTACCCCCA GAAGGTGAGG CACTATGAGT TCTACAAAAT GGTGATGCCA 
CCGCTGGAGC AGGAAGCATC GAGAGTTATC ACCAGTCAAG GGACTGTTGT CAAATACATG 
TTGGATGGAT CCACACAGAT TCTCTTTGCA GATGGGACTG TGAGCAGTAG TCCCGATTCA 
GGTCCTGTCA GTCTTCCTAC TGACGTGCCG GCCAGTGCTC ACAGTGCTGA CTTGATGGAC 
ACAATTTCTC AGCAGAAATC AGAAACAGCA CCATCGGAGA CTGTCATCAC AAAGAAAGGA 
AAAATTCAGA AAAATCAGTC ATCTATGGCA CATAAAAATG AAAGCCATGA ACTTCCCCCA 
GAAACAGTTC AAACAATAAC TCCTATGGAT GTTCACTTAG GCACCTGGTT TACAACTACA 
CCTCAAGGAA ATCGGATTGG CACCAAAGGA TCAGAAAGAA TAGCAGACTT GGCCCCATTT 
TTATCCTTTA AAGCCACAGA TCCTATCAAT GGAACGGTTA TGACAACTCG AGAAGACAAA 
GTGATCATCA TTGAAAAGAA AGATGGTACT CGGATCGTGG ATCATGCTGA TGGTACCAGA 
ATCACAACCT TTTATCAAGT TTATGAAGAT CCGACTATTT CTTCGGAGGA TCAAGAGACA 
GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG GTGAAGTGCA TGCGGATTGA AAGCTCGCAC 
TACGCCACCA TCATCACCAA CTGCGAGGAC AGTAGCTGCT GTGCCACCTT TGCGGACGGG 
ACGTCCATCA TTGCAAAGCC ACAGGGAACG TACCAGGTGT TACCACCAAA CACAGGCTGT 
CTTCATATTG CTAGGGACTG TTCAGCTATA TATTGCCATG AATCAAGCAG TGACATGTAC 
CATCCTTTTC AAAAACGTGA GCAGCTGAGA GCTAGCAGGT ACATCATGAA ACACACATCA 
GAGGTTATCT GCGAGGTCAT GGATCCTGAA GGAAATACCT TTCAGGTCAT GGCTGATGGT 
AGTGCATCAA CCATCCTACC TGAAAACAAC TTGGAAGATG ATTTAAACGC AAAAGCTGAA 
GGTTATGACA GTTTGTCATC CACTCACCTT CATAAGAATC ATCTGCAAAT CTATGGTGAA 
CATGTCCCCA GGTTTTTTGT TATCTATGCT GATGGATCAG GAGTGGAGCT TCTTCGGGAC 
AGTGACATAG AAGAGTACCT CTCCTTGGCA TATGGAGAAT CAACTACCGT AGTTCTTCAA 
GAGCCAGTAC AGGAACAACC AGGTGCCCTG AGCATCACCG TCCTTCGCCC TTTCCATGAA 
GTGTCGCCGT GGCTAATGAA GAAAGAATTA GATACGATTG TTCCTCCTAA TCTCCAGTCA 
AGGACGTGGG AGACCTTTCC CTCAGTTCAG AAAAAAACTC CAGGACCTCC GTTTGGCACT 
CAGATTTGGA AGGGCCTCGA CATTGGCTCC AAACAATTAG TGAACACTCC AGCCCCTATA 
CTGGAGAGCC CCAATGTGCT ACAGATACGC CAGTTCATCC AGCATGAGGT CATAAAGAAT 
GAGGTGAAAC TGAAACTGCA GCTTTCCCTG AAGGACTATA TAAACCATAT TCTCAAGAAA 
GAAGATGAGT TGCAGGAAAT GACAGTTAAA GATTCCAGAA CTGAGGAGGA GAGAGGCAAC 
GCTGCAGATC TCCTCAAGCT GGTTATGTCT TTCCCTAAAA TGGAGGAAAC TACAAAAAGT 
CATGTTACTG AAGTTGCAGC CCACCTAACT GATCTGTTTA AGCAGTCTTT GGCTAATGCT 
ATGGAATGTC CACCAGAAAC CTTTGAGACA GATTTCCTTG AAAAGAAATT GAGGCACACA 
GAGACGACAA AGCACTGGAA AGAAAAGTTA GCTCAAAAGA GGAAGGAAAT TGAAAAAACA 
CAGAACTATC TAAAGGAAAT TAGGAGCAAA GCAATATCAC CCTACTTCAA ATCTGAACTG 
AGCAAGCTTT ATCAGTCTCA GTTTAATTAT CTGGACACCC TTTCCAAAAA CTTGCCTCCT 
TTTACAAAGA AAGATGAGGA TGCAAGCAAA GAAGCCGCTC AAGATACATC CAGTCTTCCT 
CCAGATTCCC AGAAAAATAA AGTTTCAAAA CAGGAATCCT CAGATATACT TAGTGTTTCA 
AAATCAGAGA ACCTTGAAGA TACCAAGGAG CCTGCTAGCC AAAAGCAAAC TGAAAATTTA 
ACAGTATCTA TCAAAGAACC AGAATCGTGT ATGCCTCTGA AAATCCCAAC CCAGTCAATG 
CTGCAGGATG TGGCAGGACA AGCAAGAAAA CAGAAAGTGA GGCTACCTCA CTACTTGCTG 
AGTTCCAAGC CCAAGTCTCA GCCTCTCACA AAGACGCAAG ATTCTGTTGG AGGAAGAGTG 
AGAACATCCT CTGTGGCATC TGCTGCCATT AATAATATAA ATTCATCTCC CTTTGGATTC 
CATCTTCTCC CACCCGTGGT GAAATTTGGA GTGCTCGAAG AAGGACACAC GTATGCCACC 
ACTGTAAAGC TCAAGAATGT TGGAGTGGAC TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA 
CCCAGCACAG GACTGAAAGT GACTTACAAA CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA 
GACCTGAGAG TGGAGTTATT CGCCATGGCT GTCGGGGAGG ATGGCACCAA GGGACTGGCT 
CACATCTCTC ACAATATAGA GATTATGACT GAGCATGACG TCCTGTTCTT ACCTGTGGAA 
GCAACAGTTT TAACAAGCAG CAATTATGAT AAGAGACCAA AAGATTTTCC CCAGGGAAAA 
GAAAATCCCA CGGTCCAGAG GATTTCTACA ATTTCTTCCT CTGCACTCGG AGTCTTCATG 
TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012001559.1
CDS	125..6808
Translation	MAPKKEKGGTTTTGSKIWEPSLIAAQFNQNDWKGSIAFVVGNRIEDEVLIHALTLAVQVPQRRLFSMVSWQDIVQQVNGSSAVFGSTSKKAKKPLGGNAPLYSEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKLKDQQRRESEKKVIEDRFKIEKDKVKAKSPKEKKSPSAKVAKGKGKDQTEANTAVKKTTQLVRRGEEDDIKQYIDDEPDDGAQHYFIVVGFNNPQLLAIMTELGIPISSVIKISSENYEPLQTHLAAVSQQQESFLQPEEIEAEKLKKENGIKELEIFWKYLEPILNNQKPETNLFDVAQLEYTVKADKFPSDWSDSEMLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEEGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLPPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_012146169.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012146169.2

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AGGCGGGACA ACCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTCATA GAAGACAGAT TTAAGATAGA AAAGGATAAA 
GTGAAAGCAA AATCTCCCAA GGAGAAGAAG TCCCCATCTG CCAAGGTTGC CAAAGGAAAG 
GGAAAGGACC AGACAGAGGC AAACACTGCC GTGAAAAAGA CCACCCAGTT AGTGAGGAGG 
GGGGAAGAGG ATGACATCAA ACAATACATT GACGATGAGC CAGATGACGG TGCCCAGCAT 
TACTTTATAG TCGTGGGCTT CAACAATCCT CAGCTATTAG CGATCATGAC GGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGACACAC 
CTGGCAGCAG TTAGCCAACA GCAGGAAAGT TTTCTTCAGC CAGAAGAAAT AGAAGCTGAA 
AAACTGAAGA AAGAGAATGG CATAAAAGAA CTTGAAATCT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ACCAGAAACC AGAAACAAAT CTCTTTGATG TCGCTCAGCT TGAATACACG 
GTCAAGGCTG ACAAGTTTCC TTCTGACTGG TCAGACAGCG AGATGTTGCT GGGATTGGGC 
ACCGAAATTT TTGAAAATGT TGCCTGTCTG ATGTACGACA CCCTGGATTG GAAAAGGCAG 
CACCAGCACT ATTTGGAAAG CATGCGGCTT ATTAACGTCC CACAGGTGGT TAGTGAGAAG 
TCTGTCTTTG AAGTGACGTT GATTCCTGAG CCTCCACAAC CTGTTGTTGC AATCCCTGGA 
AAGAAGAAAA CGCAACCATA TGAAGAGCCA CAGATTCCAG CTCCTCCTCC AGCAACACTG 
CTACCACCAC CACCAGTGGC TTCCGTCATC ACAAATGAAG TAGACATGAG ATACTACAAT 
GATTTGCTGA ATCCAATCCC AGAAGAATTC ATTTCTGTGC CCTTGATACT GCATTGTATG 
CTGGAACAGG TTGTAGCAAG TGAAGAAGAT CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT 
CCCAGGGCAG ATGGGTTGGA CTACAGAATA GCAGCTCACA TCGTATCCAT CTTGCCCTCT 
CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT CTCCATGAAA TATTCTTATC TGAAGAAGAA 
AATGAAAGCA AACCAGTGCA AACAGGCCCT CTCCTGCTTA ACTATCATGA TGCCCACGCC 
CACAAGAAGT ACATGCTAAA GGACCTAAAG AATTTTGATC CAGTTCAAAT TGAACAAGAG 
ATGCAGTCCA AGTTGCCACT GTGGGAATTC CTTAAATTCC CTCTACCTCC GCCATGGAAT 
AGCACGAAAC GTCTAGCTAC AATTCATGAG CTCATGCACT TTTGTACAAG TGAAGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAA GTGTTTACTT TTGAGAGCCT GAAACTCTCT 
AAGGTTGATG AAGATGGAAA TCTAAAGCAT TCTAAAATGA TGTATGGGAC AGATTCTGAG 
ATGTTCAACA TACCATGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAACAGTAC 
ATCATGAAAA TGAATACCCA AGAAGCCAAA CAGCAAACAG ATGGTGGAAC CAAAGACAGA 
ATTTTATTTC TGAATCAGAA TTGGTCAACA CCTATGCCAG ATGATGAGCT CAACAAAAAA 
CCATCAGATT ATCGCCAGCC TGGTAGTAAC AAGAAGTCCT TAGACTCTGA TAATAGACAA 
CCAACAGAGC AGGAGACAAA CTTGAAGTCT CAGCTCCAAC CTGAGACTCT GGAGCAGACC 
ATGAACAATG AGATCAAAGA TGAAGCGGTC ACAAATGCTG ATCTTCTTGA AAAGAAACCA 
AAGAAGATGA CTGTAGAAGC CGAGTTAGAG GACATAAAGA AAACCCAGCA ACGCAATCTA 
ATGGACTGGA GTTTTACTGA ACATTTTAAA CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA 
GCCAGTCAGC AATACAGGTG TGTTGATTCT TATTACCATA CACAAGACAA CTCGTTACTG 
TTGGTCTTTC ATAATCCAAT GAATTTACAG CGTCTGCATC ACGAATATTG GAACGCTGGC 
CTTCACTCTA ATGTTGGATT CAGAAATTAT TTGGAACTTG TTGCAAAATC CATTCAAGAT 
TGGATCATAA AAGAAGAAGC TATATATCAG GAAACTAAAA TGTCTGATGA AATCAATAAA 
ACTAAGTATG AGCTGGAAAT AAAATCTTCT GTTGCTACCA AAGTTTCTTC TACTAGCAAA 
ATTTATTCTA TTAAGAAATC TAAAAGTAAC AAAGGAATGA GCAAAACAGA AATATCAGAT 
CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA AAGATTAATT TTATTTTAGA AGGCTCTCTC 
AAGGCCTGGA AAGAAGAACA AGATCGGTTA GCTGAAGAGG AACGCTTAAA GGAAGAAAAA 
AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA 
GAAGATAGTA AGATTTTGAA GAAAAAATCT TTTATAAAGG AGAAATTGAA AGAAGAGCAA 
ATGAAGATCC CGGAAGTAAC AGAGGAGCCC CTCCAGGAAC CAGAACCTGA GATAGTTTAC 
CCGTTTCATG GATACAACAT GGGAGAAATA CCTGTGCAAA TCTCAGGAAC AAATTATTAT 
CTGTATCCTT CCGATGGAGG GCAAATTGAA GTAGAAAAGA CAACATTTGA AAAAGGTCCA 
ACAGTTATCA AAATGAAAGT GAAAAAGGAC AAGCACAATT TTTTAATTCA CTTAAGAGAC 
CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA GAAGAGGATT ATCCTTCAGA AGATGAGAAA 
GAAGAAGGAG GAGAGCAAAA CTTCAAAGCA GAAGAATCAA ATAAAGAGAA TAAAGCTGTC 
AGCAAGTTTG GATCTTTTTC TGCCACCTTG GAAAATGGAA TCTGCCTCTC AATAAGTTAC 
TATGGATCAA GTGGATCAGC ACCAGAGGAT AAGGATCCTA ACTTGGAATC TATGTTGAAT 
ATCCCATCAG TACATACTCC AACAGTAATT CCTGTTACAG TGAGTATTCC TCAAGGCAAG 
GGAAAAGGGA AAACAAAAGG CAAAGAAAAA CCCAAAGAAT CTCTTAAAGA AGAGGAACAC 
CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA GAACCAGAGC CTGTTTTACA AGAGAGTCCT 
CGTGTTCCCA TCTTCCAAAA CCTGAATGTG TCTTGCCCCA ATGGGCTCCA AGTGACCTTC 
ACTGGGCAAG AGTCCCCAGA TCAATATATT GTAGGTGAGG AACCCACTTG GGATATCATG 
GTCCGACAGA ACTACCCCCA GAAGGTGAGG CACTATGAGT TCTACAAAAT GGTGATGCCA 
CCGCTGGAGC AGGAAGCATC GAGAGTTATC ACCAGTCAAG GGACTGTTGT CAAATACATG 
TTGGATGGAT CCACACAGAT TCTCTTTGCA GATGGGACTG TGAGCAGTAG TCCCGATTCA 
GGTCCTGTCA GTCTTCCTAC TGACGTGCCG GCCAGTGCTC ACAGTGCTGA CTTGATGGAC 
ACAATTTCTC AGCAGAAATC AGAAACAGCA CCATCGGAGA CTGTCATCAC AAAGAAAGGA 
AAAATTCAGA AAAATCAGTC ATCTATGGCA CATAAAAATG AAAGCCATGA ACTTCCCCCA 
GAAACAGTTC AAACAATAAC TCCTATGGAT GTTCACTTAG GCACCTGGTT TACAACTACA 
CCTCAAGGAA ATCGGATTGG CACCAAAGGA TCAGAAAGAA TAGCAGACTT GGCCCCATTT 
TTATCCTTTA AAGCCACAGA TCCTATCAAT GGAACGGTTA TGACAACTCG AGAAGACAAA 
GTGATCATCA TTGAAAAGAA AGATGGTACT CGGATCGTGG ATCATGCTGA TGGTACCAGA 
ATCACAACCT TTTATCAAGT TTATGAAGAT CCGACTATTT CTTCGGAGGA TCAAGAGACA 
GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG GTGAAGTGCA TGCGGATTGA AAGCTCGCAC 
TACGCCACCA TCATCACCAA CTGCGAGGAC AGTAGCTGCT GTGCCACCTT TGCGGACGGG 
ACGTCCATCA TTGCAAAGCC ACAGGGAACG TACCAGGTGT TACCACCAAA CACAGGCTGT 
CTTCATATTG CTAGGGACTG TTCAGCTATA TATTGCCATG AATCAAGCAG TGACATGTAC 
CATCCTTTTC AAAAACGTGA GCAGCTGAGA GCTAGCAGGT ACATCATGAA ACACACATCA 
GAGGTTATCT GCGAGGTCAT GGATCCTGAA GGAAATACCT TTCAGGTCAT GGCTGATGGT 
AGTGCATCAA CCATCCTACC TGAAAACAAC TTGGAAGATG ATTTAAACGC AAAAGCTGAA 
GGTTATGACA GTTTGTCATC CACTCACCTT CATAAGAATC ATCTGCAAAT CTATGGTGAA 
CATGTCCCCA GGTTTTTTGT TATCTATGCT GATGGATCAG GAGTGGAGCT TCTTCGGGAC 
AGTGACATAG AAGAGTACCT CTCCTTGGCA TATGGAGAAT CAACTACCGT AGTTCTTCAA 
GAGCCAGTAC AGGAACAACC AGGTGCCCTG AGCATCACCG TCCTTCGCCC TTTCCATGAA 
GTGTCGCCGT GGCTAATGAA GAAAGAATTA GATACGATTG TTCCTCCTAA TCTCCAGTCA 
AGGACGTGGG AGACCTTTCC CTCAGTTCAG AAAAAAACTC CAGGACCTCC GTTTGGCACT 
CAGATTTGGA AGGGCCTCGA CATTGGCTCC AAACAATTAG TGAACACTCC AGCCCCTATA 
CTGGAGAGCC CCAATGTGCT ACAGATACGC CAGTTCATCC AGCATGAGGT CATAAAGAAT 
GAGGTGAAAC TGAAACTGCA GCTTTCCCTG AAGGACTATA TAAACCATAT TCTCAAGAAA 
GAAGATGAGT TGCAGGAAAT GACAGTTAAA GATTCCAGAA CTGAGGAGGA GAGAGGCAAC 
GCTGCAGATC TCCTCAAGCT GGTTATGTCT TTCCCTAAAA TGGAGGAAAC TACAAAAAGT 
CATGTTACTG AAGTTGCAGC CCACCTAACT GATCTGTTTA AGCAGTCTTT GGCTAATGCT 
ATGGAATGTC CACCAGAAAC CTTTGAGACA GATTTCCTTG AAAAGAAATT GAGGCACACA 
GAGACGACAA AGCACTGGAA AGAAAAGTTA GCTCAAAAGA GGAAGGAAAT TGAAAAAACA 
CAGAACTATC TAAAGGAAAT TAGGAGCAAA GCAATATCAC CCTACTTCAA ATCTGAACTG 
AGCAAGCTTT ATCAGTCTCA GTTTAATTAT CTGGACACCC TTTCCAAAAA CTTGCCTCCT 
TTTACAAAGA AAGATGAGGA TGCAAGCAAA GAAGCCGCTC AAGATACATC CAGTCTTCCT 
CCAGATTCCC AGAAAAATAA AGTTTCAAAA CAGGAATCCT CAGATATACT TAGTGTTTCA 
AAATCAGAGA ACCTTGAAGA TACCAAGGAG CCTGCTAGCC AAAAGCAAAC TGAAAATTTA 
ACAGTATCTA TCAAAGAACC AGAATCGTGT ATGCCTCTGA AAATCCCAAC CCAGTCAATG 
CTGCAGGATG TGGCAGGACA AGCAAGAAAA CAGAAAGTGA GGCTACCTCA CTACTTGCTG 
AGTTCCAAGC CCAAGTCTCA GCCTCTCACA AAGACGCAAG ATTCTGTTGG AGGAAGAGTG 
AGAACATCCT CTGTGGCATC TGCTGCCATT AATAATATAA ATTCATCTCC CTTTGGATTC 
CATCTTCTCC CACCCGTGGT GAAATTTGGA GTGCTCGAAG AAGGACACAC GTATGCCACC 
ACTGTAAAGC TCAAGAATGT TGGAGTGGAC TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA 
CCCAGCACAG GACTGAAAGT GACTTACAAA CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA 
GACCTGAGAG TGGAGTTATT CGCCATGGCT GTCGGGGAGG ATGGCACCAA GGGACTGGCT 
CACATCTCTC ACAATATAGA GATTATGACT GAGCATGACG TCCTGTTCTT ACCTGTGGAA 
GCAACAGTTT TAACAAGCAG CAATTATGAT AAGAGACCAA AAGATTTTCC CCAGGGAAAA 
GAAAATCCCA CGGTCCAGAG GATTTCTACA ATTTCTTCCT CTGCACTCGG AGTCTTCATG 
TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_004002384.3 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_004002433.1 Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

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CloneID	OSm00812
Clone ID Related Accession (Same CDS sequence)	XM_004002384.3
Accession Version	XM_004002384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	sperm-associated antigen 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639010.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004002384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AGGCGGGACA GCCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTCATA GAAGACAGAT TTAAGATAGA AAAGGATAAA 
GTGAAAGCAA AATCTCCCAA GGAGAAGAAG TCCCCATCTG CCAAGGTTGC CAAAGGAAAG 
GGAAAGGACC AGACAGAGGC AAACACTGCC GTGAAAAAGA CCACCCAGTT AGTGAGGAGG 
GGGGAAGAGG ATGACATCAA ACAATACATT GACGATGAGC CAGATGACGG TGCCCAGCAT 
TACTTTATAG TCGTGGGCTT CAACAATCCT CAGCTATTAG CGATCATGAC GGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGACACAC 
CTGGCAGCAG TTAGCCAACA GCAGGAAAGT TTTCTTCAGC CAGAAGAAAT AGAAGCTGAA 
AAACTGAAGA AAGAGAATGG CATAAAAGAA CTTGAAATCT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ACCAGAAACC AGAAACAAAT CTCTTTGATG TCGCTCAGCT TGAATACACG 
GTCAAGGCTG ACAAGTTTCC TTCTGACTGG TCAGACAGCG AGATGTTGCT GGGATTGGGC 
ACCGAAATTT TTGAAAATGT TGCCTGTCTG ATGTACGACA CCCTGGATTG GAAAAGGCAG 
CACCAGCACT ATTTGGAAAG CATGCGGCTT ATTAACGTCC CACAGGTGGT TAGTGAGAAG 
TCTGTCTTTG AAGTGACGTT GATTCCTGAG CCTCCACAAC CTGTTGTTGC AATCCCTGGA 
AAGAAGAAAA CGCAACCATA TGAAGAGCCA CAGATTCCAG CTCCTCCTCC AGCAACACTG 
CTACCACCAC CACCAGTGGC TTCCGTCATC ACAAATGAAG TAGACATGAG ATACTACAAT 
GATTTGCTGA ATCCAATCCC AGAAGAATTC ATTTCTGTGC CCTTGATACT GCATTGTATG 
CTGGAACAGG TTGTAGCAAG TGAAGAAGAT CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT 
CCCAGGGCAG ATGGGTTGGA CTACAGAATA GCAGCTCACA TCGTATCCAT CTTGCCCTCT 
CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT CTCCATGAAA TATTCTTATC TGAAGAAGAA 
AATGAAAGCA AACCAGTGCA AACAGGCCCT CTCCTGCTTA ACTATCATGA TGCCCACGCC 
CACAAGAAGT ACATGCTAAA GGACCTAAAG AATTTTGATC CAGTTCAAAT TGAACAAGAG 
ATGCAGTCCA AGTTGCCACT GTGGGAATTC CTTAAATTCC CTCTGCCTCC GCCATGGAAT 
AGCACGAAAC GTCTAGCTAC AATTCATGAG CTCATGCACT TTTGTACAAG TGAAGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAA GTGTTTACTT TTGAGAGCCT GAAACTCTCT 
AAGGTTGATG AAGATGGAAA TCTAAAGCAT TCTAAAATGA TGTATGGGAC AGATTCTGAG 
ATGTTCAACA TACCATGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAACAGTAC 
ATCATGAAAA TGAATACCCA AGAAGCCAAA CAGCAAACAG ATGGTGGAAC CAAAGACAGA 
ATTTTATTTC TGAATCAGAA TTGGTCAACA CCTATGCCAG ATGATGAGCT CAACAAAAAA 
CCATCAGATT ATCGCCAGCC TGGTAGTAAC AAGAAGTCCT TAGACTCTGA TAATAGACAA 
CCAACAGAGC AGGAGACAAA CTTGAAGTCT CAACTCCAAC CTGAGACTCT GGAGCAGACC 
ATGAACAATG AGATCAAAGA TGAAGCGGTC ACAAATGCTG ATCTTCTTGA AAAGAAACCA 
AAGAAGATGA CTGTAGAAGC CGAGTTAGAG GACATAAAGA AAACCCAGCA ACGCAATCTA 
ATGGACTGGA GTTTTACTGA ACATTTTAAA CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA 
GCCAGTCAGC AATACAGGTG TGTTGATTCT TATTACCATA CACAAGACAA CTCGTTACTG 
TTGGTCTTTC ATAATCCAAT GAATTTACAG CGTCTGCATC ACGAATATTG GAACGCTGGC 
CTTCACTCTA ATGTTGGATT CAGAAATTAT TTGGAACTTG TTGCAAAATC CATTCAAGAT 
TGGATCATAA AAGAAGAAGC TATATATCAG GAAACTAAAA TGTCTGATGA AATCAATAAA 
ACTAAGTATG AGCTGGAAAT AAAATCTTCT GTTGCTACCA AAGTTTCTTC TACTAGCAAA 
ATTTATTCTA TTAAGAAATC TAAAAGTAAC AAAGGAATGA GCAAAACAGA AATATCAGAT 
CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA AAGATTAATT TTATTTTAGA AGGCTCTCTC 
AAGGCCTGGA AAGAAGAACA AGATCGGTTA GCTGAAGAGG AACGCTTAAA GGAAGAAAAA 
AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA 
GAAGATAGTA AGATTTTGAA GAAAAAATCT TTTATAAAGG AGAAATTGAA AGAAGAGCAA 
ATGAAGATCC CGGAAGTAAC AGAGGAGCCC CTCCAGGAAC CAGAACCTGA GATAGTTTAC 
CCGTTTCATG GATACAACAT GGGAGAAATA CCTGTGCAAA TCTCAGGAAC AAATTATTAT 
CTGTATCCTT CCGATGGAGG GCAAATTGAA GTAGAAAAGA CAACATTTGA AAAAGGTCCA 
ACAGTTATCA AAATGAAAGT GAAAAAGGAC AAGCACAATT TTTTAATTCA CTTAAGAGAC 
CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA GAAGAGGATT ATCCTTCAGA AGATGAGAAA 
GAAGGAGGAG GAGAGCAAAA CTTCAAAGCA GAAGAATCAA ATAAAGAGAA TAAAGCTGTC 
AGCAAGTTTG GATCTTTTTC TGCCACCTTG GAAAATGGAA TCTGCCTCTC AATAAGTTAC 
TATGGATCAA GTGGATCAGC ACCAGAGGAT AAGGATCCTA ACTTGGAATC TATGTTGAAT 
ATCCCATCAG TACATACTCC AACAGTAATT CCTGTTACAG TGAGTATTCC TCAAGGCAAG 
GGAAAAGGGA AAACAAAAGG CAAAGAAAAA CCCAAAGAAT CTCTTAAAGA AGAGGAGCAC 
CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA GAACCAGAGC CTGTTTTACA AGAGAGTCCT 
CGTGTTCCCA TCTTCCAAAA CCTGAATGTG TCTTGCCCCA ATGGGCTCCA AGTGACCTTC 
ACTGGGCAAG AGTCCCCAGA TCAATATATT GTAGGTGAGG AACCCACTTG GGATATCATG 
GTCCGACAGA ACTACCCCCA GAAGGTGAGG CACTATGAGT TCTACAAAAT GGTGATGCCA 
CCGCTGGAGC AGGAAGCATC GAGAGTTATC ACCAGTCAAG GGACTGTTGT CAAATACATG 
TTGGATGGAT CCACACAGAT TCTCTTTGCA GATGGGACTG TGAGCAGTAG TCCCGATTCA 
GGTCCTGTCA GTCTTCCTAC TGACGTGCCG GCCAGTGCTC ACAGTGCTGA CTTGATGGAC 
ACAATTTCTC AGCAGAAATC AGAAACAGCA CCATCGGAGA CTGTCATCAC AAAGAAAGGA 
AAAATTCAGA AAAATCAGTC ATCTATGGCA CATAAAAATG AAAGCCATGA ACTTCCCCCA 
GAAACAGTTC AAACAATAAC TCCTATGGAT GTTCACTTAG GCACCTGGTT TACAACTACA 
CCTCAAGGAA ATCGGATTGG CACCAAAGGA TCAGAAAGAA TAGCAGACTT GGCCCCATTT 
TTATCCTTTA AAGCCACAGA TCCTATCAAT GGAACGGTTA TGACAACTCG AGAAGACAAA 
GTGATCATCA TTGAAAAGAA AGATGGTACT CGGATCGTGG ATCATGCTGA TGGTACCAGA 
ATCACAACCT TTTATCAAGT TTATGAAGAT CCGACTATTT CTTCGGAGGA TCAAGAGACA 
GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG GTGAAGTGCA TGCGGATTGA AAGCTCACAC 
TACGCCACCA TCATCACCAA CTGCGAGGAC AGTAGCTGCT GTGCCACCTT TGCGGACGGG 
ACGTCCATCA TTGCAAAGCC ACAGGGAACG TACCAGGTGT TACCACCAAA CACAGGCTGT 
CTTCATATTG CTAGGGACTG TTCAGCTATA TATTGCCATG AATCAAGCAG TGACATGTAC 
CATCCTTTTC AAAAACGTGA GCAGCTGAGA GCTAGCAGGT ACATCATGAA ACACACATCA 
GAGGTTATCT GTGAGGTCAT GGATCCTGAA GGAAATACCT TTCAGGTCAT GGCTGATGGT 
AGTGCATCAA CCATCCTACC TGAAAACAAC TTGGAAGATG ATTTAAACGC AAAAGCTGAA 
GGTTATGACA GTTTGTCATC CACTCACCTT CATAAGAATC ATCTGCAAAT CTATGGTGAA 
CATGTCCCCA GGTTTTTTGT TATCTACGCT GATGGATCAG GAGTGGAGCT TCTTCGGGAC 
AGTGACATAG AAGAGTACCT CTCCTTGGCA TATGGAGAAT CAACTACCGT AGTTCTTCAA 
GAGCCAGTAC AGGAACAACC AGGTGCCCTG AGCATCACCG TCCTTCGCCC TTTCCATGAA 
GTGTCGCCGT GGCTAATGAA GAAAGAATTA GATACGATTG TTCCTCCTAA TCTCCAGTCA 
AGGACGTGGG AGACCTTTCC CTCAGTTCAG AAAAAAACTC CAGGACCTCC GTTTGGCACT 
CAGATTTGGA AGGGCCTCGA CATTGGCTCC AAACAATTAG TGAACACTCC AGCCCCTATA 
CTGGAGAGCC CCAATGTGCT ACAGATACGC CAGTTCATCC AGCATGAGGT CATAAAGAAT 
GAGGTGAAAC TGAAACTGCA GCTTTCCCTG AAGGACTATA TAAACCATAT TCTCAAGAAA 
GAAGATGAGT TGCAGGAAAT GACAGTTAAA GATTCCAGAA CTGAGGAGGA GAGAGGCAAC 
GCTGCAGATC TCCTCAAGCT GGTTATGTCT TTCCCTAAAA TGGAGGAAAC TACAAAAAGT 
CATGTTACTG AAGTTGCAGC CCACCTAACT GATCTGTTTA AGCAGTCTTT GGCTAATGCT 
ATGGAATGTC CACCAGAAAC CTTTGAGACA GATTTCCTTG AAAAGAAATT GAGGCACACA 
GAGACGACAA AGCACTGGAA AGAAAAGTTA GCTCAAAAGA GGAAGGAAAT TGAAAAAACA 
CAGAACTATC TAAAGGAAAT TAGGAGCAAA GCAATATCAC CCTACTTCAA ATCTGAACTG 
AGCAAGCTTT ATCAGTCTCA GTTTAATTAT CTGGACACCC TTTCCAAAAA CTTGCCTCCT 
TTTACAAAGA AAGATGAGGA TGCAAGCAAA GAAGCTGCTC AAGATACATC CAGTCTTCTT 
CCAGATTCCC AGAAAAATAA AGTTTCAAAA CAGGAATCCT CAGATATACT TAGTGTTTCA 
AAATCAGAGA ACCTTGAAGA TACCAAGGAG CCTGCTAGCC AAAAGCAAAC TGAAAATTTA 
ACAGTATCTA TCAAAGAACC AGAATCGTGT ATGCCTCTGA AAATCCCAAC CCAGTCAATG 
CTGCAGGATG TGGCAGGACA AGCAAGAAAA CAGAAAGTGA GGCTACCTCA CTACTTGCTG 
AGTTCCAAGC CCAAGTCTCA GCCTCTCACA AAGACGCAAG ATTCTGTTGG AGGAAGAGTG 
AGAACATCCT CTGTGGCATC TGCTGCCATT AATAATATAA ATTCATCTCC CTTTGGATTC 
CATCTTCTCC CACCCGTGGT GAAATTTGGA GTGCTTGAAG AAGGACACAC GTATGCCACC 
ACTGTAAAGC TCAAGAATGT TGGAGTGGAC TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA 
CCCAGCACAG GACTGAAAGT GACTTACAAA CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA 
GACCTGAGAG TGGAGTTATT CGCCATGGCT GTCGGGGAGG ATGGCACCAA GGGACTGGCT 
CACATCTCTC ACAATATAGA GATTATGACT GAGCATGACG TCCTGTTCTT ACCTGTGGAA 
GCAACAGTTT TAACAAGCAG CAATTATGAT AAGAGACCAA AAGATTTTCC CCAGGGAAAA 
GAAAATCCCA CGGTCCAGAG GATTTCTACA ATTTCTTCCT CTGCACTCGG AGTCTTCATG 
TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004002433.1
CDS	126..6809
Translation	MAPKKEKGGTATTGSKIWEPSLIAAQFNQNDWKGSIAFVVGNRIEDEVLIHALTLAVQVPQRRLFSMVSWQDIVQQVNGSSAVFGSTSKKAKKPLGGNAPLYSEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKLKDQQRRESEKKVIEDRFKIEKDKVKAKSPKEKKSPSAKVAKGKGKDQTEANTAVKKTTQLVRRGEEDDIKQYIDDEPDDGAQHYFIVVGFNNPQLLAIMTELGIPISSVIKISSENYEPLQTHLAAVSQQQESFLQPEEIEAEKLKKENGIKELEIFWKYLEPILNNQKPETNLFDVAQLEYTVKADKFPSDWSDSEMLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASVITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEGGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLLPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_004002384.3
Organism	Ovis aries(sheep)
Definition	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004002384.3

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AGGCGGGACA GCCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTCATA GAAGACAGAT TTAAGATAGA AAAGGATAAA 
GTGAAAGCAA AATCTCCCAA GGAGAAGAAG TCCCCATCTG CCAAGGTTGC CAAAGGAAAG 
GGAAAGGACC AGACAGAGGC AAACACTGCC GTGAAAAAGA CCACCCAGTT AGTGAGGAGG 
GGGGAAGAGG ATGACATCAA ACAATACATT GACGATGAGC CAGATGACGG TGCCCAGCAT 
TACTTTATAG TCGTGGGCTT CAACAATCCT CAGCTATTAG CGATCATGAC GGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGACACAC 
CTGGCAGCAG TTAGCCAACA GCAGGAAAGT TTTCTTCAGC CAGAAGAAAT AGAAGCTGAA 
AAACTGAAGA AAGAGAATGG CATAAAAGAA CTTGAAATCT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ACCAGAAACC AGAAACAAAT CTCTTTGATG TCGCTCAGCT TGAATACACG 
GTCAAGGCTG ACAAGTTTCC TTCTGACTGG TCAGACAGCG AGATGTTGCT GGGATTGGGC 
ACCGAAATTT TTGAAAATGT TGCCTGTCTG ATGTACGACA CCCTGGATTG GAAAAGGCAG 
CACCAGCACT ATTTGGAAAG CATGCGGCTT ATTAACGTCC CACAGGTGGT TAGTGAGAAG 
TCTGTCTTTG AAGTGACGTT GATTCCTGAG CCTCCACAAC CTGTTGTTGC AATCCCTGGA 
AAGAAGAAAA CGCAACCATA TGAAGAGCCA CAGATTCCAG CTCCTCCTCC AGCAACACTG 
CTACCACCAC CACCAGTGGC TTCCGTCATC ACAAATGAAG TAGACATGAG ATACTACAAT 
GATTTGCTGA ATCCAATCCC AGAAGAATTC ATTTCTGTGC CCTTGATACT GCATTGTATG 
CTGGAACAGG TTGTAGCAAG TGAAGAAGAT CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT 
CCCAGGGCAG ATGGGTTGGA CTACAGAATA GCAGCTCACA TCGTATCCAT CTTGCCCTCT 
CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT CTCCATGAAA TATTCTTATC TGAAGAAGAA 
AATGAAAGCA AACCAGTGCA AACAGGCCCT CTCCTGCTTA ACTATCATGA TGCCCACGCC 
CACAAGAAGT ACATGCTAAA GGACCTAAAG AATTTTGATC CAGTTCAAAT TGAACAAGAG 
ATGCAGTCCA AGTTGCCACT GTGGGAATTC CTTAAATTCC CTCTGCCTCC GCCATGGAAT 
AGCACGAAAC GTCTAGCTAC AATTCATGAG CTCATGCACT TTTGTACAAG TGAAGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAA GTGTTTACTT TTGAGAGCCT GAAACTCTCT 
AAGGTTGATG AAGATGGAAA TCTAAAGCAT TCTAAAATGA TGTATGGGAC AGATTCTGAG 
ATGTTCAACA TACCATGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAACAGTAC 
ATCATGAAAA TGAATACCCA AGAAGCCAAA CAGCAAACAG ATGGTGGAAC CAAAGACAGA 
ATTTTATTTC TGAATCAGAA TTGGTCAACA CCTATGCCAG ATGATGAGCT CAACAAAAAA 
CCATCAGATT ATCGCCAGCC TGGTAGTAAC AAGAAGTCCT TAGACTCTGA TAATAGACAA 
CCAACAGAGC AGGAGACAAA CTTGAAGTCT CAACTCCAAC CTGAGACTCT GGAGCAGACC 
ATGAACAATG AGATCAAAGA TGAAGCGGTC ACAAATGCTG ATCTTCTTGA AAAGAAACCA 
AAGAAGATGA CTGTAGAAGC CGAGTTAGAG GACATAAAGA AAACCCAGCA ACGCAATCTA 
ATGGACTGGA GTTTTACTGA ACATTTTAAA CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA 
GCCAGTCAGC AATACAGGTG TGTTGATTCT TATTACCATA CACAAGACAA CTCGTTACTG 
TTGGTCTTTC ATAATCCAAT GAATTTACAG CGTCTGCATC ACGAATATTG GAACGCTGGC 
CTTCACTCTA ATGTTGGATT CAGAAATTAT TTGGAACTTG TTGCAAAATC CATTCAAGAT 
TGGATCATAA AAGAAGAAGC TATATATCAG GAAACTAAAA TGTCTGATGA AATCAATAAA 
ACTAAGTATG AGCTGGAAAT AAAATCTTCT GTTGCTACCA AAGTTTCTTC TACTAGCAAA 
ATTTATTCTA TTAAGAAATC TAAAAGTAAC AAAGGAATGA GCAAAACAGA AATATCAGAT 
CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA AAGATTAATT TTATTTTAGA AGGCTCTCTC 
AAGGCCTGGA AAGAAGAACA AGATCGGTTA GCTGAAGAGG AACGCTTAAA GGAAGAAAAA 
AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA 
GAAGATAGTA AGATTTTGAA GAAAAAATCT TTTATAAAGG AGAAATTGAA AGAAGAGCAA 
ATGAAGATCC CGGAAGTAAC AGAGGAGCCC CTCCAGGAAC CAGAACCTGA GATAGTTTAC 
CCGTTTCATG GATACAACAT GGGAGAAATA CCTGTGCAAA TCTCAGGAAC AAATTATTAT 
CTGTATCCTT CCGATGGAGG GCAAATTGAA GTAGAAAAGA CAACATTTGA AAAAGGTCCA 
ACAGTTATCA AAATGAAAGT GAAAAAGGAC AAGCACAATT TTTTAATTCA CTTAAGAGAC 
CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA GAAGAGGATT ATCCTTCAGA AGATGAGAAA 
GAAGGAGGAG GAGAGCAAAA CTTCAAAGCA GAAGAATCAA ATAAAGAGAA TAAAGCTGTC 
AGCAAGTTTG GATCTTTTTC TGCCACCTTG GAAAATGGAA TCTGCCTCTC AATAAGTTAC 
TATGGATCAA GTGGATCAGC ACCAGAGGAT AAGGATCCTA ACTTGGAATC TATGTTGAAT 
ATCCCATCAG TACATACTCC AACAGTAATT CCTGTTACAG TGAGTATTCC TCAAGGCAAG 
GGAAAAGGGA AAACAAAAGG CAAAGAAAAA CCCAAAGAAT CTCTTAAAGA AGAGGAGCAC 
CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA GAACCAGAGC CTGTTTTACA AGAGAGTCCT 
CGTGTTCCCA TCTTCCAAAA CCTGAATGTG TCTTGCCCCA ATGGGCTCCA AGTGACCTTC 
ACTGGGCAAG AGTCCCCAGA TCAATATATT GTAGGTGAGG AACCCACTTG GGATATCATG 
GTCCGACAGA ACTACCCCCA GAAGGTGAGG CACTATGAGT TCTACAAAAT GGTGATGCCA 
CCGCTGGAGC AGGAAGCATC GAGAGTTATC ACCAGTCAAG GGACTGTTGT CAAATACATG 
TTGGATGGAT CCACACAGAT TCTCTTTGCA GATGGGACTG TGAGCAGTAG TCCCGATTCA 
GGTCCTGTCA GTCTTCCTAC TGACGTGCCG GCCAGTGCTC ACAGTGCTGA CTTGATGGAC 
ACAATTTCTC AGCAGAAATC AGAAACAGCA CCATCGGAGA CTGTCATCAC AAAGAAAGGA 
AAAATTCAGA AAAATCAGTC ATCTATGGCA CATAAAAATG AAAGCCATGA ACTTCCCCCA 
GAAACAGTTC AAACAATAAC TCCTATGGAT GTTCACTTAG GCACCTGGTT TACAACTACA 
CCTCAAGGAA ATCGGATTGG CACCAAAGGA TCAGAAAGAA TAGCAGACTT GGCCCCATTT 
TTATCCTTTA AAGCCACAGA TCCTATCAAT GGAACGGTTA TGACAACTCG AGAAGACAAA 
GTGATCATCA TTGAAAAGAA AGATGGTACT CGGATCGTGG ATCATGCTGA TGGTACCAGA 
ATCACAACCT TTTATCAAGT TTATGAAGAT CCGACTATTT CTTCGGAGGA TCAAGAGACA 
GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG GTGAAGTGCA TGCGGATTGA AAGCTCACAC 
TACGCCACCA TCATCACCAA CTGCGAGGAC AGTAGCTGCT GTGCCACCTT TGCGGACGGG 
ACGTCCATCA TTGCAAAGCC ACAGGGAACG TACCAGGTGT TACCACCAAA CACAGGCTGT 
CTTCATATTG CTAGGGACTG TTCAGCTATA TATTGCCATG AATCAAGCAG TGACATGTAC 
CATCCTTTTC AAAAACGTGA GCAGCTGAGA GCTAGCAGGT ACATCATGAA ACACACATCA 
GAGGTTATCT GTGAGGTCAT GGATCCTGAA GGAAATACCT TTCAGGTCAT GGCTGATGGT 
AGTGCATCAA CCATCCTACC TGAAAACAAC TTGGAAGATG ATTTAAACGC AAAAGCTGAA 
GGTTATGACA GTTTGTCATC CACTCACCTT CATAAGAATC ATCTGCAAAT CTATGGTGAA 
CATGTCCCCA GGTTTTTTGT TATCTACGCT GATGGATCAG GAGTGGAGCT TCTTCGGGAC 
AGTGACATAG AAGAGTACCT CTCCTTGGCA TATGGAGAAT CAACTACCGT AGTTCTTCAA 
GAGCCAGTAC AGGAACAACC AGGTGCCCTG AGCATCACCG TCCTTCGCCC TTTCCATGAA 
GTGTCGCCGT GGCTAATGAA GAAAGAATTA GATACGATTG TTCCTCCTAA TCTCCAGTCA 
AGGACGTGGG AGACCTTTCC CTCAGTTCAG AAAAAAACTC CAGGACCTCC GTTTGGCACT 
CAGATTTGGA AGGGCCTCGA CATTGGCTCC AAACAATTAG TGAACACTCC AGCCCCTATA 
CTGGAGAGCC CCAATGTGCT ACAGATACGC CAGTTCATCC AGCATGAGGT CATAAAGAAT 
GAGGTGAAAC TGAAACTGCA GCTTTCCCTG AAGGACTATA TAAACCATAT TCTCAAGAAA 
GAAGATGAGT TGCAGGAAAT GACAGTTAAA GATTCCAGAA CTGAGGAGGA GAGAGGCAAC 
GCTGCAGATC TCCTCAAGCT GGTTATGTCT TTCCCTAAAA TGGAGGAAAC TACAAAAAGT 
CATGTTACTG AAGTTGCAGC CCACCTAACT GATCTGTTTA AGCAGTCTTT GGCTAATGCT 
ATGGAATGTC CACCAGAAAC CTTTGAGACA GATTTCCTTG AAAAGAAATT GAGGCACACA 
GAGACGACAA AGCACTGGAA AGAAAAGTTA GCTCAAAAGA GGAAGGAAAT TGAAAAAACA 
CAGAACTATC TAAAGGAAAT TAGGAGCAAA GCAATATCAC CCTACTTCAA ATCTGAACTG 
AGCAAGCTTT ATCAGTCTCA GTTTAATTAT CTGGACACCC TTTCCAAAAA CTTGCCTCCT 
TTTACAAAGA AAGATGAGGA TGCAAGCAAA GAAGCTGCTC AAGATACATC CAGTCTTCTT 
CCAGATTCCC AGAAAAATAA AGTTTCAAAA CAGGAATCCT CAGATATACT TAGTGTTTCA 
AAATCAGAGA ACCTTGAAGA TACCAAGGAG CCTGCTAGCC AAAAGCAAAC TGAAAATTTA 
ACAGTATCTA TCAAAGAACC AGAATCGTGT ATGCCTCTGA AAATCCCAAC CCAGTCAATG 
CTGCAGGATG TGGCAGGACA AGCAAGAAAA CAGAAAGTGA GGCTACCTCA CTACTTGCTG 
AGTTCCAAGC CCAAGTCTCA GCCTCTCACA AAGACGCAAG ATTCTGTTGG AGGAAGAGTG 
AGAACATCCT CTGTGGCATC TGCTGCCATT AATAATATAA ATTCATCTCC CTTTGGATTC 
CATCTTCTCC CACCCGTGGT GAAATTTGGA GTGCTTGAAG AAGGACACAC GTATGCCACC 
ACTGTAAAGC TCAAGAATGT TGGAGTGGAC TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA 
CCCAGCACAG GACTGAAAGT GACTTACAAA CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA 
GACCTGAGAG TGGAGTTATT CGCCATGGCT GTCGGGGAGG ATGGCACCAA GGGACTGGCT 
CACATCTCTC ACAATATAGA GATTATGACT GAGCATGACG TCCTGTTCTT ACCTGTGGAA 
GCAACAGTTT TAACAAGCAG CAATTATGAT AAGAGACCAA AAGATTTTCC CCAGGGAAAA 
GAAAATCCCA CGGTCCAGAG GATTTCTACA ATTTCTTCCT CTGCACTCGG AGTCTTCATG 
TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_004002384.4 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_004002433.2 Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

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CloneID	OSm57767
Clone ID Related Accession (Same CDS sequence)	XM_004002384.4
Accession Version	XM_004002384.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	sperm-associated antigen 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004002384.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AGGCGGGACA GCCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTCATA GAAGACAGAT TTAAGATAGA AAAGGATAAA 
GTGAAAGCAA AATCTCCCAA GGAGAAGAAG TCCCCATCTG CCAAGGTTGC CAAAGGAAAG 
GGAAAGGACC AGACAGAGGC AAACACTGCC GTGAAAAAGA CCACCCAGTT AGTGAGGAGG 
GGGGAAGAGG ATGACATCAA ACAATACATT GACGATGAGC CAGATGACGG TGCCCAGCAT 
TACTTTATAG TCGTGGGCTT CAACAATCCT CAGCTATTAG CGATCATGAC GGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGACACAC 
CTGGCAGCAG TTAGCCAACA GCAGGAAAGT TTTCTTCAGC CAGAAGAAAT AGAAGCTGAA 
AAACTGAAGA AAGAGAATGG CATAAAAGAA CTTGAAATCT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ACCAGAAACC AGAAACAAAT CTCTTTGATG TCGCTCAGCT TGAATACACA 
GTCAAGGCTG ACAAGTTTCC TTCTGACTGG TCAGACAGCG AGATGTTGCT GGGATTGGGC 
ACCGAAATTT TTGAAAATGT TGCCTGTCTG ATGTACGACA CCCTGGATTG GAAAAGGCAG 
CACCAGCACT ATTTGGAAAG CATGCGGCTT ATTAACGTCC CACAGGTGGT TAGTGAGAAG 
TCTGTCTTTG AAGTGACGTT GATTCCTGAG CCTCCACAAC CTGTTGTTGC AATCCCTGGA 
AAGAAGAAAA CACAACCATA TGAAGAGCCA CAGATTCCAG CTCCTCCTCC AGCAACACTG 
CTACCACCAC CACCAGTGGC TTCCATCATC ACAAATGAAG TAGACATGAG ATACTACAAT 
GATTTGCTGA ATCCAATCCC AGAAGAATTC ATTTCTGTGC CCTTGATACT GCATTGTATG 
CTGGAACAGG TTGTAGCAAG TGAAGAAGAT CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT 
CCCAGGGCAG ATGGGTTGGA CTACAGAATA GCAGCTCACA TCGTATCCAT CTTGCCCTCT 
CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT CTCCATGAAA TATTCTTATC TGAAGAAGAA 
AATGAAAGCA AACCAGTGCA AACAGGCCCT CTCCTGCTTA ACTATCATGA TGCCCACGCC 
CACAAGAAGT ACATGCTAAA GGACCTAAAG AATTTTGATC CAGTTCAAAT TGAACAAGAG 
ATGCAGTCCA AGTTGCCACT GTGGGAATTC CTTAAATTCC CTCTACCTCC GCCATGGAAT 
AGCACGAAAC GTCTAGCTAC AATTCATGAG CTCATGCACT TTTGTACAAG TGAAGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAA GTGTTTACTT TTGAGAGCCT GAAACTCTCT 
AAGGTTGATG AAGATGGAAA TCTAAAGCAT TCTAAAATGA TGTATGGGAC AGATTCTGAG 
ATGTTCAACA TACCATGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAACAGTAC 
ATCATGAAAA TGAATACCCA AGAAGCCAAA CAGCAAACAG ATGGTGGAAC CAAAGACAGA 
ATTTTATTTC TGAATCAGAA TTGGTCAACA CCTATGCCAG ATGATGAGCT CAACAAAAAA 
CCATCAGATT ATCGCCAGCC TGGTAGTAAC AAGAAGTCCT TAGACTCTGA TAATAGACAA 
CCAACAGAGC AGGAGACAAA CTTGAAGTCT CAGCTCCAAC CTGAGACTCT GGAGCAGACC 
ATGAACAATG AGATCAAAGA TGAAGCGGTC ACAAATGCTG ATCTTCTTGA AAAGAAACCA 
AAGAAGATGA CTGTAGAAGC CGAGTTAGAG GACATAAAGA AAACCCAGCA ACGCAATCTA 
ATGGACTGGA GTTTTACTGA ACATTTTAAA CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA 
GCCAGTCAGC AATACAGGTG TGTTGATTCT TATTACCATA CACAAGACAA CTCGTTACTG 
TTGGTCTTTC ATAATCCAAT GAATTTACAG CGTCTGCATC ACGAATATTG GAACGCTGGC 
CTTCACTCTA ATGTTGGATT CAGAAATTAT TTGGAACTTG TTGCAAAATC CATTCAAGAT 
TGGATCATAA AAGAAGAAGC TATATATCAG GAAACTAAAA TGTCTGATGA AATCAATAAA 
ACTAAGTATG AGCTGGAAAT AAAATCTTCT GTTGCTACCA AAGTTTCTTC TACTAGCAAA 
ATTTATTCTA TTAAGAAATC TAAAAGTAAC AAAGGAATGA GCAAAACAGA AATATCAGAT 
CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA AAGATTAATT TTATTTTAGA AGGCTCTCTC 
AAGGCCTGGA AAGAAGAACA AGATCGGTTA GCTGAAGAGG AACGCTTAAA GGAAGAAAAA 
AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA 
GAAGATAGTA AGATTTTGAA GAAAAAATCT TTTATAAAGG AGAAATTGAA AGAAGAGCAA 
ATGAAGATCC CGGAAGTAAC AGAGGAGCCC CTCCAGGAAC CAGAACCTGA GATAGTTTAC 
CCGTTTCATG GATACAACAT GGGAGAAATA CCTGTGCAAA TCTCAGGAAC AAATTATTAT 
CTGTATCCTT CCGATGGAGG GCAAATTGAA GTAGAAAAGA CAACATTTGA AAAAGGTCCA 
ACAGTTATCA AAATGAAAGT GAAAAAGGAC AAGCACAATT TTTTAATTCA CTTAAGAGAC 
CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA GAAGAGGATT ATCCTTCAGA AGATGAGAAA 
GAAGAAGGAG GAGAGCAAAA CTTCAAAGCA GAAGAATCAA ATAAAGAGAA TAAAGCTGTC 
AGCAAGTTTG GATCTTTTTC TGCCACCTTG GAAAATGGAA TCTGCCTCTC AATAAGTTAC 
TATGGATCAA GTGGATCAGC ACCAGAGGAT AAGGATCCTA ACTTGGAATC TATGTTGAAT 
ATCCCATCAG TACATACTCC AACAGTAATT CCTGTTACAG TGAGTATTCC TCAAGGCAAG 
GGAAAAGGGA AAACAAAAGG CAAAGAAAAA CCCAAAGAAT CTCTTAAAGA AGAGGAGCAC 
CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA GAACCAGAGC CTGTTTTACA AGAGAGTCCT 
CGTGTTCCCA TCTTCCAAAA CCTGAATGTG TCTTGCCCCA ATGGGCTCCA AGTGACCTTC 
ACTGGGCAAG AGTCCCCAGA TCAATATATT GTAGGTGAGG AACCCACTTG GGATATCATG 
GTCCGACAGA ACTACCCCCA GAAGGTGAGG CACTATGAGT TCTACAAAAT GGTGATGCCA 
CCGCTGGAGC AGGAAGCATC GAGAGTTATC ACCAGTCAAG GGACTGTTGT CAAATACATG 
CTGGATGGAT CCACACAGAT TCTCTTTGCA GATGGGACTG TGAGCAGTAG TCCCGATTCA 
GGTCCTGTCA GTCTTCCTAC TGACGTGCCG GCCAGTGCTC ACAGTGCTGA CTTGATGGAC 
ACAATTTCTC AGCAGAAATC AGAAACAGCA CCATCGGAGA CTGTCATCAC AAAGAAAGGA 
AAAATTCAGA AAAATCAGTC ATCTATGGCA CATAAAAATG AAAGCCATGA ACTTCCCCCA 
GAAACAGTTC AAACAATAAC TCCTATGGAT GTTCACTTAG GCACCTGGTT TACAACTACA 
CCTCAAGGAA ATCGGATTGG CACCAAAGGA TCAGAAAGAA TAGCAGACTT GGCCCCATTT 
TTATCCTTTA AAGCCACAGA TCCTATCAAT GGAACGGTTA TGACAACTCG AGAAGACAAA 
GTGATCATCA TTGAAAAGAA AGATGGTACT CGGATCGTGG ATCATGCTGA TGGTACCAGA 
ATCACAACCT TTTATCAAGT TTATGAAGAT CCGACTATTT CTTCGGAGGA TCAAGAGACA 
GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG GTGAAGTGCA TGCGGATTGA AAGCTCACAC 
TACGCCACCA TCATCACCAA CTGCGAGGAC AGTAGCTGCT GTGCCACCTT TGCGGACGGG 
ACGTCCATCA TTGCAAAGCC ACAGGGAACG TACCAGGTGT TACCACCAAA CACAGGCTGT 
CTTCATATTG CTAGGGACTG TTCAGCTATA TATTGCCATG AATCAAGCAG TGACATGTAC 
CATCCTTTTC AAAAACGTGA GCAGCTGAGA GCTAGCAGGT ACATCATGAA ACACACATCA 
GAGGTTATCT GCGAGGTCAT GGATCCTGAA GGAAATACCT TTCAGGTCAT GGCTGATGGT 
AGTGCATCAA CCATCCTACC TGAAAACAAC TTGGAAGATG ATTTAAACGC AAAAGCTGAA 
GGTTATGACA GTTTGTCATC CACTCACCTT CATAAGAATC ATCTGCAAAT CTATGGTGAA 
CATGTCCCCA GGTTTTTTGT TATCTATGCT GATGGATCAG GAGTGGAGCT TCTTCGGGAC 
AGTGACATAG AAGAGTACCT CTCCTTGGCA TATGGAGAAT CAACTACCGT AGTTCTTCAA 
GAGCCAGTAC AGGAACAACC AGGTGCCCTG AGCATCACCG TCCTTCGCCC TTTCCATGAA 
GTGTCGCCGT GGCTAATGAA GAAAGAATTA GATACGATTG TTCCTCCTAA TCTCCAGTCA 
AGGACGTGGG AGACCTTTCC CTCAGTTCAG AAAAAAACTC CAGGACCTCC GTTTGGCACT 
CAGATTTGGA AGGGCCTCGA CATTGGCTCC AAACAATTAG TGAACACTCC AGCCCCTATA 
CTGGAGAGCC CCAATGTGCT ACAGATACGC CAGTTCATCC AGCATGAGGT CATAAAGAAT 
GAGGTGAAAC TGAAACTGCA GCTTTCCCTG AAGGACTATA TAAACCATAT TCTCAAGAAA 
GAAGATGAGT TGCAGGAAAT GACAGTTAAA GATTCCAGAA CTGAGGAGGA GAGAGGCAAC 
GCTGCAGATC TCCTCAAGCT GGTTATGTCT TTCCCTAAAA TGGAGGAAAC TACAAAAAGT 
CATGTTACTG AAGTTGCAGC CCACCTAACT GATCTGTTTA AGCAGTCTTT GGCTAATGCT 
ATGGAATGTC CACCAGAAAC CTTTGAGACA GATTTCCTTG AAAAGAAATT GAGGCACACA 
GAGACGACAA AGCACTGGAA AGAAAAGTTA GCTCAAAAGA GGAAGGAAAT TGAAAAAACA 
CAGAACTATC TAAAGGAAAT TAGGAGCAAA GCAATATCAC CCTACTTCAA ATCTGAACTG 
AGCAAGCTTT ATCAGTCTCA GTTTAATTAT CTGGACACCC TTTCCAAAAA CTTGCCTCCT 
TTTACAAAGA AAGATGAGGA TGCAAGCAAA GAAGCCGCTC AAGATACATC CAGTCTTCCT 
CCAGATTCCC AGAAAAATAA AGTTTCAAAA CAGGAATCCT CAGATATACT TAGTGTTTCA 
AAATCAGAGA ACCTTGAAGA TACCAAGGAG CCTGCTAGCC AAAAGCAAAC TGAAAATTTA 
ACAGTATCTA TCAAAGAACC AGAATCGTGT ATGCCTCTGA AAATCCCAAC CCAGTCAATG 
CTGCAGGATG TGGCAGGACA AGCAAGAAAA CAGAAAGTGA GGCTGCCTCA CTACTTGCTG 
AGTTCCAAGC CCAAGTCTCA GCCTCTCACA AAGACGCAAG ATTCTGTGGG AGGAAGAGTG 
AGAACATCCT CTGTGGCATC TGCTGCCATT AATAATATAA ATTCATCTCC CTTTGGATTC 
CATCTTCTCC CACCCGTGGT GAAATTTGGA GTGCTCGAAG AAGGACACAC GTATGCCACC 
ACTGTAAAGC TCAAGAATGT TGGAGTGGAC TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA 
CCCAGCACAG GACTGAAAGT GACTTACAAA CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA 
GACCTGAGAG TGGAGTTATT CGCCATGGCT GTCGGGGAGG ATGGCACCAA GGGACTGGCT 
CACATCTCTC ACAATATAGA GATTATGACT GAGCATGACG TCCTGTTCTT ACCTGTGGAA 
GCAACAGTTT TAACAAGCAG CAATTATGAT AAGAGACCAA AAGATTTTCC CCAGGGAAAA 
GAAAATCCCA CGGTCCAGAG GATTTCTACA ATTTCTTCCT CTGCACTCGG AGTCTTCATG 
TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004002433.2
CDS	48..6731
Translation	MAPKKEKGGTATTGSKIWEPSLIAAQFNQNDWKGSIAFVVGNRIEDEVLIHALTLAVQVPQRRLFSMVSWQDIVQQVNGSSAVFGSTSKKAKKPLGGNAPLYSEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKLKDQQRRESEKKVIEDRFKIEKDKVKAKSPKEKKSPSAKVAKGKGKDQTEANTAVKKTTQLVRRGEEDDIKQYIDDEPDDGAQHYFIVVGFNNPQLLAIMTELGIPISSVIKISSENYEPLQTHLAAVSQQQESFLQPEEIEAEKLKKENGIKELEIFWKYLEPILNNQKPETNLFDVAQLEYTVKADKFPSDWSDSEMLLGLGTEIFENVACLMYDTLDWKRQHQHYLESMRLINVPQVVSEKSVFEVTLIPEPPQPVVAIPGKKKTQPYEEPQIPAPPPATLLPPPPVASIITNEVDMRYYNDLLNPIPEEFISVPLILHCMLEQVVASEEDLVPPSLVELPPRADGLDYRIAAHIVSILPSLCLTEREKKNLHEIFLSEEENESKPVQTGPLLLNYHDAHAHKKYMLKDLKNFDPVQIEQEMQSKLPLWEFLKFPLPPPWNSTKRLATIHELMHFCTSEVLSWNEVERAFKVFTFESLKLSKVDEDGNLKHSKMMYGTDSEMFNIPWDNPARFAKQIRQQYIMKMNTQEAKQQTDGGTKDRILFLNQNWSTPMPDDELNKKPSDYRQPGSNKKSLDSDNRQPTEQETNLKSQLQPETLEQTMNNEIKDEAVTNADLLEKKPKKMTVEAELEDIKKTQQRNLMDWSFTEHFKPKVLLQVLQEASQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLHHEYWNAGLHSNVGFRNYLELVAKSIQDWIIKEEAIYQETKMSDEINKTKYELEIKSSVATKVSSTSKIYSIKKSKSNKGMSKTEISDQEKDKEKEKEKINFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKEAGKKKGKEKLEKEDSKILKKKSFIKEKLKEEQMKIPEVTEEPLQEPEPEIVYPFHGYNMGEIPVQISGTNYYLYPSDGGQIEVEKTTFEKGPTVIKMKVKKDKHNFLIHLRDPRKIVEKEKKEEDYPSEDEKEEGGEQNFKAEESNKENKAVSKFGSFSATLENGICLSISYYGSSGSAPEDKDPNLESMLNIPSVHTPTVIPVTVSIPQGKGKGKTKGKEKPKESLKEEEHPKEEEKKEVEEPEPVLQESPRVPIFQNLNVSCPNGLQVTFTGQESPDQYIVGEEPTWDIMVRQNYPQKVRHYEFYKMVMPPLEQEASRVITSQGTVVKYMLDGSTQILFADGTVSSSPDSGPVSLPTDVPASAHSADLMDTISQQKSETAPSETVITKKGKIQKNQSSMAHKNESHELPPETVQTITPMDVHLGTWFTTTPQGNRIGTKGSERIADLAPFLSFKATDPINGTVMTTREDKVIIIEKKDGTRIVDHADGTRITTFYQVYEDPTISSEDQETAEGPQRIARQVKCMRIESSHYATIITNCEDSSCCATFADGTSIIAKPQGTYQVLPPNTGCLHIARDCSAIYCHESSSDMYHPFQKREQLRASRYIMKHTSEVICEVMDPEGNTFQVMADGSASTILPENNLEDDLNAKAEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEVSPWLMKKELDTIVPPNLQSRTWETFPSVQKKTPGPPFGTQIWKGLDIGSKQLVNTPAPILESPNVLQIRQFIQHEVIKNEVKLKLQLSLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLANAMECPPETFETDFLEKKLRHTETTKHWKEKLAQKRKEIEKTQNYLKEIRSKAISPYFKSELSKLYQSQFNYLDTLSKNLPPFTKKDEDASKEAAQDTSSLPPDSQKNKVSKQESSDILSVSKSENLEDTKEPASQKQTENLTVSIKEPESCMPLKIPTQSMLQDVAGQARKQKVRLPHYLLSSKPKSQPLTKTQDSVGGRVRTSSVASAAINNINSSPFGFHLLPPVVKFGVLEEGHTYATTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQADLRVELFAMAVGEDGTKGLAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRPKDFPQGKENPTVQRISTISSSALGVFMSRKISPH

Target ORF information:

RefSeq Version	XM_004002384.4
Organism	Ovis aries(sheep)
Definition	Ovis aries sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004002384.4

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AGGCGGGACA GCCACCACCG GCTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAATT CAACCAGAAC GACTGGAAAG GCTCCATCGC TTTTGTCGTT 
GGAAACCGGA TCGAGGACGA GGTTCTCATT CACGCTCTCA CCCTCGCTGT CCAAGTTCCT 
CAGCGCAGAC TCTTCAGTAT GGTCTCCTGG CAAGACATCG TCCAGCAGGT CAATGGATCA 
AGTGCAGTTT TTGGAAGTAC ATCTAAAAAG GCAAAGAAAC CTCTAGGTGG TAATGCTCCC 
TTATATTCTG AGGTGTTAAC GGCAGCAAAA GCAATTATGG ACAGCGGAGA GAAATTAACC 
TTACCACTGA TAGGGAAACT CTTGAAATTT CAGCTTCTTC AGATTAAGTT GAAAGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTCATA GAAGACAGAT TTAAGATAGA AAAGGATAAA 
GTGAAAGCAA AATCTCCCAA GGAGAAGAAG TCCCCATCTG CCAAGGTTGC CAAAGGAAAG 
GGAAAGGACC AGACAGAGGC AAACACTGCC GTGAAAAAGA CCACCCAGTT AGTGAGGAGG 
GGGGAAGAGG ATGACATCAA ACAATACATT GACGATGAGC CAGATGACGG TGCCCAGCAT 
TACTTTATAG TCGTGGGCTT CAACAATCCT CAGCTATTAG CGATCATGAC GGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGACACAC 
CTGGCAGCAG TTAGCCAACA GCAGGAAAGT TTTCTTCAGC CAGAAGAAAT AGAAGCTGAA 
AAACTGAAGA AAGAGAATGG CATAAAAGAA CTTGAAATCT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ACCAGAAACC AGAAACAAAT CTCTTTGATG TCGCTCAGCT TGAATACACA 
GTCAAGGCTG ACAAGTTTCC TTCTGACTGG TCAGACAGCG AGATGTTGCT GGGATTGGGC 
ACCGAAATTT TTGAAAATGT TGCCTGTCTG ATGTACGACA CCCTGGATTG GAAAAGGCAG 
CACCAGCACT ATTTGGAAAG CATGCGGCTT ATTAACGTCC CACAGGTGGT TAGTGAGAAG 
TCTGTCTTTG AAGTGACGTT GATTCCTGAG CCTCCACAAC CTGTTGTTGC AATCCCTGGA 
AAGAAGAAAA CACAACCATA TGAAGAGCCA CAGATTCCAG CTCCTCCTCC AGCAACACTG 
CTACCACCAC CACCAGTGGC TTCCATCATC ACAAATGAAG TAGACATGAG ATACTACAAT 
GATTTGCTGA ATCCAATCCC AGAAGAATTC ATTTCTGTGC CCTTGATACT GCATTGTATG 
CTGGAACAGG TTGTAGCAAG TGAAGAAGAT CTTGTCCCAC CAAGTCTTGT AGAGCTGCCT 
CCCAGGGCAG ATGGGTTGGA CTACAGAATA GCAGCTCACA TCGTATCCAT CTTGCCCTCT 
CTCTGTCTGA CAGAGAGGGA GAAAAAGAAT CTCCATGAAA TATTCTTATC TGAAGAAGAA 
AATGAAAGCA AACCAGTGCA AACAGGCCCT CTCCTGCTTA ACTATCATGA TGCCCACGCC 
CACAAGAAGT ACATGCTAAA GGACCTAAAG AATTTTGATC CAGTTCAAAT TGAACAAGAG 
ATGCAGTCCA AGTTGCCACT GTGGGAATTC CTTAAATTCC CTCTACCTCC GCCATGGAAT 
AGCACGAAAC GTCTAGCTAC AATTCATGAG CTCATGCACT TTTGTACAAG TGAAGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAA GTGTTTACTT TTGAGAGCCT GAAACTCTCT 
AAGGTTGATG AAGATGGAAA TCTAAAGCAT TCTAAAATGA TGTATGGGAC AGATTCTGAG 
ATGTTCAACA TACCATGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAACAGTAC 
ATCATGAAAA TGAATACCCA AGAAGCCAAA CAGCAAACAG ATGGTGGAAC CAAAGACAGA 
ATTTTATTTC TGAATCAGAA TTGGTCAACA CCTATGCCAG ATGATGAGCT CAACAAAAAA 
CCATCAGATT ATCGCCAGCC TGGTAGTAAC AAGAAGTCCT TAGACTCTGA TAATAGACAA 
CCAACAGAGC AGGAGACAAA CTTGAAGTCT CAGCTCCAAC CTGAGACTCT GGAGCAGACC 
ATGAACAATG AGATCAAAGA TGAAGCGGTC ACAAATGCTG ATCTTCTTGA AAAGAAACCA 
AAGAAGATGA CTGTAGAAGC CGAGTTAGAG GACATAAAGA AAACCCAGCA ACGCAATCTA 
ATGGACTGGA GTTTTACTGA ACATTTTAAA CCAAAAGTTC TGCTTCAGGT TCTTCAAGAA 
GCCAGTCAGC AATACAGGTG TGTTGATTCT TATTACCATA CACAAGACAA CTCGTTACTG 
TTGGTCTTTC ATAATCCAAT GAATTTACAG CGTCTGCATC ACGAATATTG GAACGCTGGC 
CTTCACTCTA ATGTTGGATT CAGAAATTAT TTGGAACTTG TTGCAAAATC CATTCAAGAT 
TGGATCATAA AAGAAGAAGC TATATATCAG GAAACTAAAA TGTCTGATGA AATCAATAAA 
ACTAAGTATG AGCTGGAAAT AAAATCTTCT GTTGCTACCA AAGTTTCTTC TACTAGCAAA 
ATTTATTCTA TTAAGAAATC TAAAAGTAAC AAAGGAATGA GCAAAACAGA AATATCAGAT 
CAAGAAAAAG ACAAAGAAAA AGAGAAGGAA AAGATTAATT TTATTTTAGA AGGCTCTCTC 
AAGGCCTGGA AAGAAGAACA AGATCGGTTA GCTGAAGAGG AACGCTTAAA GGAAGAAAAA 
AAAGCAGAAA AGAAGAGTAA GGAGGCTGGT AAAAAGAAAG GCAAGGAAAA GCTAGAAAAA 
GAAGATAGTA AGATTTTGAA GAAAAAATCT TTTATAAAGG AGAAATTGAA AGAAGAGCAA 
ATGAAGATCC CGGAAGTAAC AGAGGAGCCC CTCCAGGAAC CAGAACCTGA GATAGTTTAC 
CCGTTTCATG GATACAACAT GGGAGAAATA CCTGTGCAAA TCTCAGGAAC AAATTATTAT 
CTGTATCCTT CCGATGGAGG GCAAATTGAA GTAGAAAAGA CAACATTTGA AAAAGGTCCA 
ACAGTTATCA AAATGAAAGT GAAAAAGGAC AAGCACAATT TTTTAATTCA CTTAAGAGAC 
CCTAGGAAAA TTGTGGAAAA GGAGAAAAAA GAAGAGGATT ATCCTTCAGA AGATGAGAAA 
GAAGAAGGAG GAGAGCAAAA CTTCAAAGCA GAAGAATCAA ATAAAGAGAA TAAAGCTGTC 
AGCAAGTTTG GATCTTTTTC TGCCACCTTG GAAAATGGAA TCTGCCTCTC AATAAGTTAC 
TATGGATCAA GTGGATCAGC ACCAGAGGAT AAGGATCCTA ACTTGGAATC TATGTTGAAT 
ATCCCATCAG TACATACTCC AACAGTAATT CCTGTTACAG TGAGTATTCC TCAAGGCAAG 
GGAAAAGGGA AAACAAAAGG CAAAGAAAAA CCCAAAGAAT CTCTTAAAGA AGAGGAGCAC 
CCAAAAGAAG AAGAGAAAAA GGAAGTAGAA GAACCAGAGC CTGTTTTACA AGAGAGTCCT 
CGTGTTCCCA TCTTCCAAAA CCTGAATGTG TCTTGCCCCA ATGGGCTCCA AGTGACCTTC 
ACTGGGCAAG AGTCCCCAGA TCAATATATT GTAGGTGAGG AACCCACTTG GGATATCATG 
GTCCGACAGA ACTACCCCCA GAAGGTGAGG CACTATGAGT TCTACAAAAT GGTGATGCCA 
CCGCTGGAGC AGGAAGCATC GAGAGTTATC ACCAGTCAAG GGACTGTTGT CAAATACATG 
CTGGATGGAT CCACACAGAT TCTCTTTGCA GATGGGACTG TGAGCAGTAG TCCCGATTCA 
GGTCCTGTCA GTCTTCCTAC TGACGTGCCG GCCAGTGCTC ACAGTGCTGA CTTGATGGAC 
ACAATTTCTC AGCAGAAATC AGAAACAGCA CCATCGGAGA CTGTCATCAC AAAGAAAGGA 
AAAATTCAGA AAAATCAGTC ATCTATGGCA CATAAAAATG AAAGCCATGA ACTTCCCCCA 
GAAACAGTTC AAACAATAAC TCCTATGGAT GTTCACTTAG GCACCTGGTT TACAACTACA 
CCTCAAGGAA ATCGGATTGG CACCAAAGGA TCAGAAAGAA TAGCAGACTT GGCCCCATTT 
TTATCCTTTA AAGCCACAGA TCCTATCAAT GGAACGGTTA TGACAACTCG AGAAGACAAA 
GTGATCATCA TTGAAAAGAA AGATGGTACT CGGATCGTGG ATCATGCTGA TGGTACCAGA 
ATCACAACCT TTTATCAAGT TTATGAAGAT CCGACTATTT CTTCGGAGGA TCAAGAGACA 
GCCGAAGGTC CTCAGAGGAT CGCCAGGCAG GTGAAGTGCA TGCGGATTGA AAGCTCACAC 
TACGCCACCA TCATCACCAA CTGCGAGGAC AGTAGCTGCT GTGCCACCTT TGCGGACGGG 
ACGTCCATCA TTGCAAAGCC ACAGGGAACG TACCAGGTGT TACCACCAAA CACAGGCTGT 
CTTCATATTG CTAGGGACTG TTCAGCTATA TATTGCCATG AATCAAGCAG TGACATGTAC 
CATCCTTTTC AAAAACGTGA GCAGCTGAGA GCTAGCAGGT ACATCATGAA ACACACATCA 
GAGGTTATCT GCGAGGTCAT GGATCCTGAA GGAAATACCT TTCAGGTCAT GGCTGATGGT 
AGTGCATCAA CCATCCTACC TGAAAACAAC TTGGAAGATG ATTTAAACGC AAAAGCTGAA 
GGTTATGACA GTTTGTCATC CACTCACCTT CATAAGAATC ATCTGCAAAT CTATGGTGAA 
CATGTCCCCA GGTTTTTTGT TATCTATGCT GATGGATCAG GAGTGGAGCT TCTTCGGGAC 
AGTGACATAG AAGAGTACCT CTCCTTGGCA TATGGAGAAT CAACTACCGT AGTTCTTCAA 
GAGCCAGTAC AGGAACAACC AGGTGCCCTG AGCATCACCG TCCTTCGCCC TTTCCATGAA 
GTGTCGCCGT GGCTAATGAA GAAAGAATTA GATACGATTG TTCCTCCTAA TCTCCAGTCA 
AGGACGTGGG AGACCTTTCC CTCAGTTCAG AAAAAAACTC CAGGACCTCC GTTTGGCACT 
CAGATTTGGA AGGGCCTCGA CATTGGCTCC AAACAATTAG TGAACACTCC AGCCCCTATA 
CTGGAGAGCC CCAATGTGCT ACAGATACGC CAGTTCATCC AGCATGAGGT CATAAAGAAT 
GAGGTGAAAC TGAAACTGCA GCTTTCCCTG AAGGACTATA TAAACCATAT TCTCAAGAAA 
GAAGATGAGT TGCAGGAAAT GACAGTTAAA GATTCCAGAA CTGAGGAGGA GAGAGGCAAC 
GCTGCAGATC TCCTCAAGCT GGTTATGTCT TTCCCTAAAA TGGAGGAAAC TACAAAAAGT 
CATGTTACTG AAGTTGCAGC CCACCTAACT GATCTGTTTA AGCAGTCTTT GGCTAATGCT 
ATGGAATGTC CACCAGAAAC CTTTGAGACA GATTTCCTTG AAAAGAAATT GAGGCACACA 
GAGACGACAA AGCACTGGAA AGAAAAGTTA GCTCAAAAGA GGAAGGAAAT TGAAAAAACA 
CAGAACTATC TAAAGGAAAT TAGGAGCAAA GCAATATCAC CCTACTTCAA ATCTGAACTG 
AGCAAGCTTT ATCAGTCTCA GTTTAATTAT CTGGACACCC TTTCCAAAAA CTTGCCTCCT 
TTTACAAAGA AAGATGAGGA TGCAAGCAAA GAAGCCGCTC AAGATACATC CAGTCTTCCT 
CCAGATTCCC AGAAAAATAA AGTTTCAAAA CAGGAATCCT CAGATATACT TAGTGTTTCA 
AAATCAGAGA ACCTTGAAGA TACCAAGGAG CCTGCTAGCC AAAAGCAAAC TGAAAATTTA 
ACAGTATCTA TCAAAGAACC AGAATCGTGT ATGCCTCTGA AAATCCCAAC CCAGTCAATG 
CTGCAGGATG TGGCAGGACA AGCAAGAAAA CAGAAAGTGA GGCTGCCTCA CTACTTGCTG 
AGTTCCAAGC CCAAGTCTCA GCCTCTCACA AAGACGCAAG ATTCTGTGGG AGGAAGAGTG 
AGAACATCCT CTGTGGCATC TGCTGCCATT AATAATATAA ATTCATCTCC CTTTGGATTC 
CATCTTCTCC CACCCGTGGT GAAATTTGGA GTGCTCGAAG AAGGACACAC GTATGCCACC 
ACTGTAAAGC TCAAGAATGT TGGAGTGGAC TTCTGCAGGT TTAGGGTGAA GCAGCCTCCA 
CCCAGCACAG GACTGAAAGT GACTTACAAA CCTGGGCCTG TGGCGGCTGG TTTGCAGGCA 
GACCTGAGAG TGGAGTTATT CGCCATGGCT GTCGGGGAGG ATGGCACCAA GGGACTGGCT 
CACATCTCTC ACAATATAGA GATTATGACT GAGCATGACG TCCTGTTCTT ACCTGTGGAA 
GCAACAGTTT TAACAAGCAG CAATTATGAT AAGAGACCAA AAGATTTTCC CCAGGGAAAA 
GAAAATCCCA CGGTCCAGAG GATTTCTACA ATTTCTTCCT CTGCACTCGG AGTCTTCATG 
TCCCGTAAAA TTTCTCCACA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPAG17
Entrez Gene ID	101109497
Full Name	sperm associated antigen 17
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPAG17 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

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