COL5A1 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A1
Entrez Gene ID	101107777
Full Name	collagen type V alpha 1 chain
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027966117.1	XP_027821918.1	collagen alpha-1(V) chain isoform X1
XM_012117953.2	XP_011973343.1	collagen alpha-1(V) chain isoform X2
XM_015094097.1	XP_014949583.1	LOW QUALITY PROTEIN: collagen alpha-1(V) chain isoform X1
XM_012117954.2	XP_011973344.1	collagen alpha-1(V) chain isoform X3
XM_027966118.1	XP_027821919.1	collagen alpha-1(V) chain isoform X2

Pathways from BioSystems
Gene Ontology

The following COL5A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm48517	XM_015094097.1 Latest version!	Ovis aries collagen, type V, alpha 1 (COL5A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OOv05939	XM_012117953.2 Latest version!	Ovis aries musimon collagen, type V, alpha 1 (COL5A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv05940	XM_012117954.2 Latest version!	Ovis aries musimon collagen, type V, alpha 1 (COL5A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm76411	XM_027966117.1 Latest version!	Ovis aries collagen type V alpha 1 chain (COL5A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm76412	XM_027966118.1 Latest version!	Ovis aries collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A1 ( XM_015094097.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014949583.1 Ovis aries collagen, type V, alpha 1 (COL5A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm48517
Clone ID Related Accession (Same CDS sequence)	XM_015094097.1
Accession Version	XM_015094097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGTCTGTGC CCGGCTCTCA GGAGCTGACC CAGCCCCCAA CAGAAGCCCC TTCGGCGCCC 
AGCACCACGG AGGGCCCTGG GAAGGAGGAG GACCCCGGCA TCGGCGACTA TGACTACGTG 
CCCAGTGAGG ACTACTACAC GCCACCCCCT TATGAGGACT TCGGCTACGG TGAAGGCATC 
GAGAACCCGG ATGAGAACCC TGACCAGCAC CCGGACCAGG GCGCCCGGGC TGAGGTTCCC 
ACCAGCACGG TCCTCACCTC CAACGGCTCC AACCCGGCCC CGCCTCCGGA GGAGGGGATG 
GACGACCTGG AGGGAGAGTT CACGGAGGAA ACCATCAAGA ACCTGGACGA GAGCTACTAC 
GACCCGTACT ACGACCCCAC CATCTCCCCA TCGGAGGTCG GGCCAGGCAT GCCCGCCAAC 
CAGGACACCA TCTATGAAGG GATCGGAGGC CCTCGGGGCG AGAAAGGCCA GAAAGGAGAG 
CCCGCCATCA TCGAGCCGGG CATGCTCCTG GAGGGGCCGC CTGGCCCCGA AGGCCCTGCG 
GGTCTCCCAG GACCTCCAGG GACGATGGGT CCCACAGGCC AAGTGGGTGA CCCTGGAGAA 
AGGGGTCCCC CTGGACGCCC AGGCCTTCCT GGCGCTGACG GGCTGCCCGG CCCTCCTGGA 
ACCATGCTCA TGCTGCCTTT CCGGTTCGGA GGGGGCGGTG ACGCGGGCTC CAAAGGACCC 
ATGGTCTCCG CGCAGGAGTC CCAAGCTCAA GCCATCCTGC AGCAGGCCCG GCTCGCGTTG 
AGGGGGCCTG CTGGCCCGAT GGGTCTCACT GGGAGACCGG GTCCCATGGG CCCTCCTGGG 
AGCGGAGGTC TGAAGGGCGA GCCAGGAGAC ATGGGGCCTC AGGGTCCACG GGGTGTGCAG 
GGCCCGCCAG CCCGAGATGG AATGACTGGG AGAAGGGGCC GAGCCGGCAG TGACGGAGCG 
AGAGGGATGC CCGGGCAGAC TGGCCCCAAG GGTGACCGCG GCTTTGATGG CCTGGCGGGG 
CTGCCAGGGG AGAAAGGCCA CCGGGGTGAC CCTGGCCCAT CCGGCCCGCC GGGACCTCCA 
GGGGAAGACG GAGAAAGGGG CGACGATGGA GAAGCTGGGC CCAGAGGGCT GCCTGGGGAG 
CCCGGGCCGC GAGGTCTGCT GGGGCCGAAG GGCCCCCCAG GCCCCCCTGG ACCTCCCGGT 
GTGACAGGAA TGGATGGTCA ACCAGGCCCG AAAGGCAACG TGGGTCCCCA GGGAGAGCCC 
GCGNCCCCGG GGCAGCAGGG CAACCCAGGT GCCCAGGGTC TTCCGGGCCC CCAGGGCGCA 
ATCGGGCCTC CAGGAGAAAA GGGTCCACTG GGCAAACCCG GCCTCCCAGG AATGCCCGGT 
GCTGATGGAC CCCCGGGGCA CCCTGGCAAA GAAGGCCCTC CAGGAGAGAA GGGAGGGCAG 
GGTCCACCTG GCCCCCAGGG TCCAATTGGC TACCCAGGTC CTCGCGGAGT CAAGGGGGCG 
GACGGCATCC GAGGTCTGAA GGGCACCAAG GGCGAGAAGG GTGAAGATGG CTTCCCGGGC 
TTCAAAGGAG ACATGGGCAT CAAGGGCGAT CGGGGGGAGA TCGGCCCACC TGGTCCCAGA 
GGGGAGGACG GTCCCGAAGG TCCCAAGGGA CGAGGCGGTC CGAACGGCGA CCCCGGTCCT 
CTGGGACCCC CTGGGGAGAA GGGCAAACTC GGAGTCCCAG GACTGCCAGG CTACCCAGGA 
AGACAGGGGC CAAAGGGTTC TATCGGATTT CCTGGATTTC CTGGCGCCAA CGGAGAAAAG 
GGCGGCAGGG GCACCCCTGG GAAGCCGGGA CCGCGGGGGC AGCGAGGCCC GACGGGCCCA 
CGAGGTGAAA GAGGCCCACG GGGCATCACC GGGAAGCCTG GCCCCAAGGG CAACTCTGGA 
GGCGATGGCC CGGCTGGCCC TCCCGGTGAA CGGGGACCCA ACGGACCCCA AGGACCCACG 
GGGTTCCCTG GACCAAAGGG CCCCCCGGGG CCCCCAGGCA AGGACGGGCT CCCGGGACAC 
CCTGGACAGC GAGGCGAGAC GGGTTTCCAA GGCAAGACCG GCCCTCCAGG CCCTCCAGGT 
GTGGTCGGCC CTCAGGGCCC CACTGGGGAA ACCGGTCCCA TGGGCGAGCG TGGTCACCCT 
GGCCCCCCAG GGCCCCCCGG TGAACAGGGG CTCNNCGGGA AAGAAGGGAC GAAGGGAGAC 
CCAGGCCCCG CCGGCCTTCC TGGGAAAGAC GGTCCCCCCG GGCTGCGAGG CTTCCCTGGA 
GACCGAGGAC TTCCTGGTCC AGTGGGGGCG CTTGGACTGA AAGGCAGTGA AGGCCCCCCC 
GGCCCGCCAG GCCCCGCGGG ATCTCCAGGG GAGAGAGGTC CTGCCGGTGC CGCCGGGCCC 
ATTGGAATTC CAGGGAGGCC CGGGCCCCAG GGGCCCCCTG GGCCAGCTGG GGAGAAAGGC 
GCCCCTGGCG AGAAGGGTCC ACAAGGCCCA GCTGGCCGAG ATGGTCTCCA GGGCCCCGTG 
GGGCTCCCCG GTCCCGCTGG CCCTGTGGGC CCCCCTGGAG AAGACGGAGA CAAGGGAGAG 
ATTGGAGAGC CAGGACAGAA GGGCAGCAAG GGAGACAAAG GCGAGCAGGG TCCACCAGGG 
CCTACAGGAC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CCGGAGCCGA TGGCGAACCG 
GGACCTCGGG GCCAGCAGGG CCTCTTTGGG CAGAAAGGCG ACGAAGGTCC AAGAGGCTTC 
CCCGGGCCCC CGGGTCCTGC GGGGCTGCAG GGTCTGCCGG GACCTCCTGG AGAGAAGGGC 
GAGACGGGTG ACGTGGGTCA GATGGGCCCG CCAGGTCCCC CCGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCAGACGG GCCACAAGGA CCCCCCGGTG GAATAGGCAA CCCCGGAGCC 
GTGGGAGAGA AGGGTGAGCC TGGCGAAGCG GGAGAGCCTG GCCTTCCCGG TGAAGGGGGC 
CCCCCGGGAC CCAAAGGCGA AAGGGGGGAG AAGGGCGAGT CGGGCCCCTC CGGTGCCGCT 
GGACCCCCGG GGCCCAAGGG CCCTCCCGGA CAGAAACTGC CTGATCCCGG CCCCGTCGGT 
TTTCCCGGAG ATCCTGGTCC CCCCGGAGAA CCTGGCCCCG CGGGTCAAGA CGGTCCTCCT 
GGCGACAAAG GAGACGACGG TGAACCCGGG CAAACGGGAT CACCAGGCCC TACGGGTGAG 
CCAGGCCCAT CTGGGCCTCC AGGAAAAAGG GGTCCGCCCG GCCCCGCAGG CCCTGAAGGC 
AGGCAGGGGG AGAAAGGAGC CAAGGGAGAA GCCGGCTTGG AAGGCCCTCC TGGGAAGACT 
GGCCCCATCG GCCCCCAGGG GGCCCCCGGG AAGCCGGGGC CTGACGGCCT TCGAGGGATC 
CCCGGCCCAG TGGGTGAACA AGGTCTCCCC GGAGCCCCGG GCCCCGATGG CCCCCCAGGA 
CCCATGGGCC CCCCAGGACT CCCCGGCCTC AAAGGAGACT CTGGGCCCAA AGGCGAAAAG 
GGTCACCCAG GCTTGATCGG CCTCATCGGA CCTCCGGGCG AACAGGGAGA AAAGGGTGAA 
CGTGGAGTCC CCGGACCCCA GGGCTCCTCG GGCCCTAAGG GAGAACAGTG CTGTGGGGGT 
GCGGGTCACA GCGGGGGCCT GGGGGGAAGG GGCCGCCCCC CACTTACGCC CTGCCCCTCT 
CCACAGGGTC CCCCTGGTCC AAAAGGTGCT AAGGGCTCCT CGGGTCCAAC TGGCCCGAAG 
GGTGAGGCAG GCCAGCCAGG ACCCCCCTCG GACCCAGGGC CCCCCCGGGC GACGGTCATC 
CAGCCCCTGC CGATCCAAGC GTCCAGGACC CGGCGGAACA TCGACGCCAG CCAGCTGATG 
GACGACGCAG AGGGCGAGCA GTACATGGAC TATGCGGACG GCATGGAGGA GATCTTCGGC 
TCGCTCAACT CCCTGAAGCT GGAGATCGAG CAGATGAAGC GGCCGCTGGG CACGCCGCAG 
AACCCCGCGC GCACCTGCAA AGACCTCCAG CTCTGCCACC CCGACTTCCC CGATGGCGAA 
TACTGGGTGG ATCCGAACCA AGGCTGCTCC CGGGACTCCT TCAAGGTCTA CTGCAACTTC 
ACGGCCGGGG GCTCGACGTG CGTCTTCCCC GACAAGAAGT CCGAGGGGGC CAGAATCACT 
TCTTGGCCCA AAGAAAACCC GGGTTCCTGG TTCAGTGAGT TCAAGCGTGG CAAACTGGTA 
AGACGGCCCC CGCCGAGCTG CGGTGTGGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014949583.1
CDS	103..4395
Translation	MSVPGSQELTQPPTEAPSAPSTTEGPGKEEDPGIGDYDYVPSEDYYTPPPYEDFGYGEGIENPDENPDQHPDQGARAEVPTSTVLTSNGSNPAPPPEEGMDDLEGEFTEETIKNLDESYYDPYYDPTISPSEVGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLLEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPARDGMTGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGEDGERGDDGEAGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPAnullPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLnullGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPAGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGQKLPDPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGAPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGERGVPGPQGSSGPKGEQCCGGAGHSGGLGGRGRPPLTPCPSPQGPPGPKGAKGSSGPTGPKGEAGQPGPPSDPGPPRATVIQPLPIQASRTRRNIDASQLMDDAEGEQYMDYADGMEEIFGSLNSLKLEIEQMKRPLGTPQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLVRRPPPSCGVV

Target ORF information:

RefSeq Version	XM_015094097.1
Organism	Ovis aries(sheep)
Definition	Ovis aries collagen, type V, alpha 1 (COL5A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015094097.1

ORF Insert Sequence:

ATGTCTGTGC CCGGCTCTCA GGAGCTGACC CAGCCCCCAA CAGAAGCCCC TTCGGCGCCC 
AGCACCACGG AGGGCCCTGG GAAGGAGGAG GACCCCGGCA TCGGCGACTA TGACTACGTG 
CCCAGTGAGG ACTACTACAC GCCACCCCCT TATGAGGACT TCGGCTACGG TGAAGGCATC 
GAGAACCCGG ATGAGAACCC TGACCAGCAC CCGGACCAGG GCGCCCGGGC TGAGGTTCCC 
ACCAGCACGG TCCTCACCTC CAACGGCTCC AACCCGGCCC CGCCTCCGGA GGAGGGGATG 
GACGACCTGG AGGGAGAGTT CACGGAGGAA ACCATCAAGA ACCTGGACGA GAGCTACTAC 
GACCCGTACT ACGACCCCAC CATCTCCCCA TCGGAGGTCG GGCCAGGCAT GCCCGCCAAC 
CAGGACACCA TCTATGAAGG GATCGGAGGC CCTCGGGGCG AGAAAGGCCA GAAAGGAGAG 
CCCGCCATCA TCGAGCCGGG CATGCTCCTG GAGGGGCCGC CTGGCCCCGA AGGCCCTGCG 
GGTCTCCCAG GACCTCCAGG GACGATGGGT CCCACAGGCC AAGTGGGTGA CCCTGGAGAA 
AGGGGTCCCC CTGGACGCCC AGGCCTTCCT GGCGCTGACG GGCTGCCCGG CCCTCCTGGA 
ACCATGCTCA TGCTGCCTTT CCGGTTCGGA GGGGGCGGTG ACGCGGGCTC CAAAGGACCC 
ATGGTCTCCG CGCAGGAGTC CCAAGCTCAA GCCATCCTGC AGCAGGCCCG GCTCGCGTTG 
AGGGGGCCTG CTGGCCCGAT GGGTCTCACT GGGAGACCGG GTCCCATGGG CCCTCCTGGG 
AGCGGAGGTC TGAAGGGCGA GCCAGGAGAC ATGGGGCCTC AGGGTCCACG GGGTGTGCAG 
GGCCCGCCAG CCCGAGATGG AATGACTGGG AGAAGGGGCC GAGCCGGCAG TGACGGAGCG 
AGAGGGATGC CCGGGCAGAC TGGCCCCAAG GGTGACCGCG GCTTTGATGG CCTGGCGGGG 
CTGCCAGGGG AGAAAGGCCA CCGGGGTGAC CCTGGCCCAT CCGGCCCGCC GGGACCTCCA 
GGGGAAGACG GAGAAAGGGG CGACGATGGA GAAGCTGGGC CCAGAGGGCT GCCTGGGGAG 
CCCGGGCCGC GAGGTCTGCT GGGGCCGAAG GGCCCCCCAG GCCCCCCTGG ACCTCCCGGT 
GTGACAGGAA TGGATGGTCA ACCAGGCCCG AAAGGCAACG TGGGTCCCCA GGGAGAGCCC 
GCGNCCCCGG GGCAGCAGGG CAACCCAGGT GCCCAGGGTC TTCCGGGCCC CCAGGGCGCA 
ATCGGGCCTC CAGGAGAAAA GGGTCCACTG GGCAAACCCG GCCTCCCAGG AATGCCCGGT 
GCTGATGGAC CCCCGGGGCA CCCTGGCAAA GAAGGCCCTC CAGGAGAGAA GGGAGGGCAG 
GGTCCACCTG GCCCCCAGGG TCCAATTGGC TACCCAGGTC CTCGCGGAGT CAAGGGGGCG 
GACGGCATCC GAGGTCTGAA GGGCACCAAG GGCGAGAAGG GTGAAGATGG CTTCCCGGGC 
TTCAAAGGAG ACATGGGCAT CAAGGGCGAT CGGGGGGAGA TCGGCCCACC TGGTCCCAGA 
GGGGAGGACG GTCCCGAAGG TCCCAAGGGA CGAGGCGGTC CGAACGGCGA CCCCGGTCCT 
CTGGGACCCC CTGGGGAGAA GGGCAAACTC GGAGTCCCAG GACTGCCAGG CTACCCAGGA 
AGACAGGGGC CAAAGGGTTC TATCGGATTT CCTGGATTTC CTGGCGCCAA CGGAGAAAAG 
GGCGGCAGGG GCACCCCTGG GAAGCCGGGA CCGCGGGGGC AGCGAGGCCC GACGGGCCCA 
CGAGGTGAAA GAGGCCCACG GGGCATCACC GGGAAGCCTG GCCCCAAGGG CAACTCTGGA 
GGCGATGGCC CGGCTGGCCC TCCCGGTGAA CGGGGACCCA ACGGACCCCA AGGACCCACG 
GGGTTCCCTG GACCAAAGGG CCCCCCGGGG CCCCCAGGCA AGGACGGGCT CCCGGGACAC 
CCTGGACAGC GAGGCGAGAC GGGTTTCCAA GGCAAGACCG GCCCTCCAGG CCCTCCAGGT 
GTGGTCGGCC CTCAGGGCCC CACTGGGGAA ACCGGTCCCA TGGGCGAGCG TGGTCACCCT 
GGCCCCCCAG GGCCCCCCGG TGAACAGGGG CTCNNCGGGA AAGAAGGGAC GAAGGGAGAC 
CCAGGCCCCG CCGGCCTTCC TGGGAAAGAC GGTCCCCCCG GGCTGCGAGG CTTCCCTGGA 
GACCGAGGAC TTCCTGGTCC AGTGGGGGCG CTTGGACTGA AAGGCAGTGA AGGCCCCCCC 
GGCCCGCCAG GCCCCGCGGG ATCTCCAGGG GAGAGAGGTC CTGCCGGTGC CGCCGGGCCC 
ATTGGAATTC CAGGGAGGCC CGGGCCCCAG GGGCCCCCTG GGCCAGCTGG GGAGAAAGGC 
GCCCCTGGCG AGAAGGGTCC ACAAGGCCCA GCTGGCCGAG ATGGTCTCCA GGGCCCCGTG 
GGGCTCCCCG GTCCCGCTGG CCCTGTGGGC CCCCCTGGAG AAGACGGAGA CAAGGGAGAG 
ATTGGAGAGC CAGGACAGAA GGGCAGCAAG GGAGACAAAG GCGAGCAGGG TCCACCAGGG 
CCTACAGGAC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CCGGAGCCGA TGGCGAACCG 
GGACCTCGGG GCCAGCAGGG CCTCTTTGGG CAGAAAGGCG ACGAAGGTCC AAGAGGCTTC 
CCCGGGCCCC CGGGTCCTGC GGGGCTGCAG GGTCTGCCGG GACCTCCTGG AGAGAAGGGC 
GAGACGGGTG ACGTGGGTCA GATGGGCCCG CCAGGTCCCC CCGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCAGACGG GCCACAAGGA CCCCCCGGTG GAATAGGCAA CCCCGGAGCC 
GTGGGAGAGA AGGGTGAGCC TGGCGAAGCG GGAGAGCCTG GCCTTCCCGG TGAAGGGGGC 
CCCCCGGGAC CCAAAGGCGA AAGGGGGGAG AAGGGCGAGT CGGGCCCCTC CGGTGCCGCT 
GGACCCCCGG GGCCCAAGGG CCCTCCCGGA CAGAAACTGC CTGATCCCGG CCCCGTCGGT 
TTTCCCGGAG ATCCTGGTCC CCCCGGAGAA CCTGGCCCCG CGGGTCAAGA CGGTCCTCCT 
GGCGACAAAG GAGACGACGG TGAACCCGGG CAAACGGGAT CACCAGGCCC TACGGGTGAG 
CCAGGCCCAT CTGGGCCTCC AGGAAAAAGG GGTCCGCCCG GCCCCGCAGG CCCTGAAGGC 
AGGCAGGGGG AGAAAGGAGC CAAGGGAGAA GCCGGCTTGG AAGGCCCTCC TGGGAAGACT 
GGCCCCATCG GCCCCCAGGG GGCCCCCGGG AAGCCGGGGC CTGACGGCCT TCGAGGGATC 
CCCGGCCCAG TGGGTGAACA AGGTCTCCCC GGAGCCCCGG GCCCCGATGG CCCCCCAGGA 
CCCATGGGCC CCCCAGGACT CCCCGGCCTC AAAGGAGACT CTGGGCCCAA AGGCGAAAAG 
GGTCACCCAG GCTTGATCGG CCTCATCGGA CCTCCGGGCG AACAGGGAGA AAAGGGTGAA 
CGTGGAGTCC CCGGACCCCA GGGCTCCTCG GGCCCTAAGG GAGAACAGTG CTGTGGGGGT 
GCGGGTCACA GCGGGGGCCT GGGGGGAAGG GGCCGCCCCC CACTTACGCC CTGCCCCTCT 
CCACAGGGTC CCCCTGGTCC AAAAGGTGCT AAGGGCTCCT CGGGTCCAAC TGGCCCGAAG 
GGTGAGGCAG GCCAGCCAGG ACCCCCCTCG GACCCAGGGC CCCCCCGGGC GACGGTCATC 
CAGCCCCTGC CGATCCAAGC GTCCAGGACC CGGCGGAACA TCGACGCCAG CCAGCTGATG 
GACGACGCAG AGGGCGAGCA GTACATGGAC TATGCGGACG GCATGGAGGA GATCTTCGGC 
TCGCTCAACT CCCTGAAGCT GGAGATCGAG CAGATGAAGC GGCCGCTGGG CACGCCGCAG 
AACCCCGCGC GCACCTGCAA AGACCTCCAG CTCTGCCACC CCGACTTCCC CGATGGCGAA 
TACTGGGTGG ATCCGAACCA AGGCTGCTCC CGGGACTCCT TCAAGGTCTA CTGCAACTTC 
ACGGCCGGGG GCTCGACGTG CGTCTTCCCC GACAAGAAGT CCGAGGGGGC CAGAATCACT 
TCTTGGCCCA AAGAAAACCC GGGTTCCTGG TTCAGTGAGT TCAAGCGTGG CAAACTGGTA 
AGACGGCCCC CGCCGAGCTG CGGTGTGGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( XM_012117953.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011973343.1 Ovis aries musimon collagen, type V, alpha 1 (COL5A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv05939
Clone ID Related Accession (Same CDS sequence)	XM_012117953.2
Accession Version	XM_012117953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	collagen alpha-1(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942270.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012117953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGTGGGCGCC GCCTCCGAGC 
CGCGCAGCTC AGCCAGCAGA TCTCCTGAAG GTTCTAGATT TTCACAACCT GCCTGATGGA 
ATAACCAAGA CCACAGGCTT CTGTGCAGCG CGGCGATCTT CCAAAGGCCC GGATGTCGCC 
TACAGGGTCA CGAAAGATGC CCAGCTCAGC GCGCCCACCA AGCAGCTGTA CCCTGCATCT 
GCGTTTCCTG AGGACTTCTC CATCCTAACC ACTGTGAAAG CCAAGAAAGG CAGCCAGGCC 
TTCCTGGTCT CCATCTATAA CGAGCAGGGC ATCCAGCAGA TCGGCCTGGA GATGGGACGC 
TCCCCGGTCT TCCTCTACGA GGACCACACG GGGAAGCCGG GGCCAGAGGA CTACCCCCTC 
TTCAGAGGCA TCAACCTGTC GGATGGCAAG TGGCACAGAA TCGCTCTCAG CATCCACAAG 
AAGAATGTCA CCTTGATCCT GGACTGTAAA AAGAAGACGA CCAAGTTCCT GGACCGCAGT 
GAACACCCTG TGATAGACGT CAACGGCATC GTTGTATTCG GCACCCGGAT TCTGGATGAG 
GAAGTGTTTG AGGGTGACAT CCAGCAGCTG CTCCTCGTCT CGGACCACCG GGCGGCTTAC 
GATTACTGTG AGCATTACAG CCCAGACTGC GACACGGCCG TCCCTGACAA ACCCCAGTCT 
CAGGACCCCA ACCCAGACGA ATACTACCCA GATGGGGACA ATGAAGGAGA CACCTATTAC 
TACGAGTACC CCTACTACGA GGACACGGAC GACACGGGCA AGGACCCCGT CCCCACCAAG 
ACGCCCGTGG AAGCTGCTCG GGAGACCACG GAGGTGCCCG AGGAGCTGAC CCAGCCCCCA 
ACAGAAGCCC CTTCGGCGCC CAGCACCACG GAGGGCCCTG GGAAGGAGGA GGACCCCGGC 
ATCGGCGACT ATGACTACGT GCCCAGTGAG GACTACTACA CGCCACCCCC TTACGAGGAC 
TTCGGCTACG GTGAAGGCAT CGAGAACCCG GATGAGAACC CCGACCAGCA CCCGGACCAG 
GGCGCCCGGG CTGAGGTTCC CACCAGCACG GTCCTCACCT CCAACGGCTC CAACCCGGCC 
CCGCCTCCGG AGGAGGGGAT GGACGACCTG GAGGGAGAGT TCACGGAGGA AACCATCAAG 
AACCTGGACG AGAGCTACTA CGACCCGTAC TACGACCCCA CCATCTCCCC ATCGGAGGTC 
GGGCCAGGCA TGCCCGCCAA CCAGGACACC ATCTATGAAG GGATCGGAGG CCCTCGGGGC 
GAGAAAGGCC AGAAAGGAGA GCCTGCCATC ATCGAGCCGG GCATGCTCCT GGAGGGGCCG 
CCTGGCCCCG AAGGCCCTGC GGGTCTCCCA GGACCTCCAG GGACGATGGG TCCCACAGGC 
CAAGTGGGTG ACCCTGGAGA AAGGGGTCCC CCTGGACGCC CAGGCCTTCC TGGCGCTGAC 
GGGCTGCCTG GCCCTCCTGG AACCATGCTC ATGCTGCCTT TCCGGTTCGG AGGGGGCGGT 
GACGCGGGCT CCAAAGGACC CATGGTCTCC GCGCAGGAGT CCCAAGCTCA AGCCATCCTG 
CAGCAGGCCC GGCTCGCGTT GAGGGGGCCT GCTGGCCCGA TGGGTCTCAC TGGGAGACCG 
GGTCCCATGG GCCCTCCTGG GAGCGGAGGT CTGAAGGGCG AGCCAGGAGA CATGGGGCCT 
CAGGGTCCAC GGGGTGTGCA GGGCCCGCCT GGCCCGGCAG GAAAGCCTGG GAGAAGGGGC 
CGAGCCGGCA GTGACGGAGC GAGAGGGATG CCCGGGCAGA CTGGCCCTAA GGGTGACCGC 
GGCTTTGATG GCCTGGCGGG GCTGCCAGGG GAGAAAGGCC ACCGGGGTGA CCCTGGCCCA 
TCCGGCCCGC CGGGACCTCC AGGGGAAGAC GGAGAAAGGG GCGACGATGG AGAAGCTGGG 
CCCAGAGGGC TGCCTGGGGA GCCCGGGCCG CGAGGTCTGC TGGGGCCGAA GGGCCCCCCA 
GGCCCCCCTG GACCTCCCGG TGTGACAGGA ATGGATGGTC AACCAGGCCC GAAAGGCAAC 
GTGGGTCCCC AGGGAGAGCC CGGCCCCCCG GGGCAGCAGG GCAACCCAGG TGCCCAGGGT 
CTTCCGGGCC CCCAGGGCGC AATCGGGCCT CCAGGAGAAA AGGGTCCACT GGGCAAACCC 
GGCCTCCCAG GAATGCCCGG TGCTGATGGA CCCCCGGGGC ACCCTGGCAA AGAAGGCCCT 
CCAGGAGAGA AGGGAGGGCA GGGTCCACCT GGCCCCCAGG GTCCAATTGG CTACCCAGGT 
CCTCGCGGAG TCAAGGGGGC GGACGGCATC CGAGGTCTGA AGGGCACCAA GGGCGAGAAG 
GGTGAAGATG GCTTCCCGGG CTTCAAAGGA GACATGGGCA TCAAGGGCGA TCGGGGGGAG 
ATCGGCCCAC CTGGTCCCAG AGGGGAGGAC GGTCCCGAAG GTCCCAAGGG ACGAGGCGGT 
CCGAACGGCG ACCCCGGTCC TCTGGGACCC CCTGGGGAGA AGGGCAAACT CGGAGTCCCA 
GGACTGCCAG GCTACCCAGG AAGACAGGGG CCAAAGGGTT CTATCGGATT TCCTGGATTT 
CCTGGCGCCA ACGGAGAAAA GGGCGGCAGG GGCACCCCTG GGAAGCCGGG ACCGCGGGGG 
CAGCGAGGCC CGACGGGCCC ACGAGGTGAA AGAGGCCCAC GGGGCATCAC CGGGAAGCCT 
GGCCCCAAGG GCAACTCTGG AGGCGATGGC CCGGCTGGCC CTCCCGGTGA ACGGGGACCC 
AACGGACCCC AAGGACCCAC GGGGTTCCCT GGACCAAAGG GCCCCCCGGG GCCCCCAGGC 
AAGGACGGGC TCCCGGGACA CCCTGGACAG CGAGGCGAGA CGGGTTTCCA AGGCAAGACC 
GGCCCTCCAG GCCCTCCAGG TGTGGTCGGC CCTCAGGGCC CCACTGGGGA AACCGGTCCC 
ATGGGCGAGC GTGGTCACCC TGGCCCCCCA GGGCCCCCCG GTGAACAGGG GCTCCCGGGC 
CTGGCCGGGA AAGAAGGGAC GAAGGGAGAC CCAGGCCCCG CCGGCCTTCC TGGGAAAGAC 
GGTCCCCCTG GGCTGCGAGG CTTCCCTGGA GACCGAGGAC TTCCTGGTCC AGTGGGGGCG 
CTTGGACTGA AAGGCAGTGA AGGCCCCCCC GGCCCGCCAG GCCCCGCGGG ATCTCCAGGG 
GAGAGAGGTC CTGCCGGTGC CGCCGGGCCC ATTGGAATTC CAGGGAGGCC CGGGCCCCAG 
GGGCCCCCTG GGCCAGCTGG GGAGAAAGGC GCCCCTGGCG AGAAGGGTCC ACAAGGCCCA 
GCTGGCCGAG ATGGTCTCCA GGGCCCCGTG GGGCTCCCCG GTCCCGCTGG CCCTGTGGGC 
CCCCCTGGAG AAGACGGAGA CAAGGGAGAG ATTGGAGAGC CAGGACAGAA GGGCAGCAAG 
GGAGACAAAG GCGAGCAGGG TCCACCAGGG CCTACAGGAC CTCAAGGCCC CATCGGACAG 
CCAGGCCCCT CCGGAGCCGA TGGCGAACCG GGACCTCGGG GCCAGCAGGG CCTCTTTGGG 
CAGAAAGGCG ACGAAGGTCC GAGAGGCTTC CCCGGGCCCC CGGGTCCTGT GGGGCTGCAG 
GGTCTGCCGG GACCTCCTGG AGAGAAGGGC GAGACGGGTG ACGTGGGTCA GATGGGCCCG 
CCAGGTCCCC CTGGCCCCCG AGGACCCTCC GGAGCTCCAG GTGCAGATGG GCCACAAGGA 
CCCCCCGGTG GAATAGGCAA CCCCGGAGCC GTGGGAGAGA AGGGTGAGCC TGGCGAAGCG 
GGAGAGCCTG GCCTTCCCGG TGAAGGGGGC CCCCCGGGAC CCAAAGGCGA AAGGGGGGAG 
AAGGGCGAGT CGGGCCCCTC CGGTGCCGCC GGACCCCCGG GGCCCAAGGG CCCTCCCGGA 
GACGACGGTC CCAAAGGCAG CCCCGGCCCC GTCGGTTTTC CCGGAGATCC TGGTCCCCCC 
GGAGAACCTG GCCCCGCGGG TCAAGACGGT CCTCCTGGCG ACAAAGGAGA CGACGGTGAA 
CCCGGGCAAA CGGGATCACC AGGCCCTACG GGTGAGCCAG GCCCATCTGG GCCTCCAGGA 
AAAAGGGGTC CGCCCGGCCC CGCAGGCCCC GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG 
GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG AAGACTGGCC CCATCGGCCC CCAGGGGGCC 
CCCGGGAAGC CGGGGCCTGA CGGCCTTCGA GGGATCCCCG GCCCAGTGGG TGAACAAGGT 
CTCCCCGGAG CCCCGGGCCC CGATGGCCCC CCAGGACCCA TGGGCCCCCC AGGACTCCCC 
GGCCTCAAAG GAGACTCTGG GCCCAAAGGA GAAAAGGGTC ACCCAGGCTT GATCGGCCTC 
ATCGGACCTC CGGGCGAACA GGGAGAAAAA GGTGAACGTG GAGTCCCCGG ACCCCAGGGC 
TCCTCGGGCC CTAAGGGAGA ACAGGGTATC ACTGGTCCTT CCGGCCCGAT TGGCCCCCCA 
GGTCCCCCCG GCCTGCCGGG CCCCCCTGGT CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA 
ACTGGCCCGA AGGGTGAGGC AGGCCAGCCA GGACCCCCTG GCCCACCAGG CCCCCCGGGC 
GAGGTCATCC AGCCCCTGCC GATCCAAGCG TCCAGGACCC GGCGGAACAT CGACGCCAGC 
CAGCTGATGG ACGACGCAGA GGGCGAGCAG TACATGGACT ATGCGGACGG CATGGAGGAG 
ATCTTCGGCT CGCTCAACTC CCTGAAGCTG GAGATCGAGC AGATGAAGCG GCCGCTGGGC 
ACGCCGCAGA ACCCCGCGCG CACCTGCAAA GACCTCCAGC TCTGCCACCC CGACTTCCCC 
GATGGCGAAT ACTGGGTGGA TCCGAACCAA GGCTGCTCCC GGGACTCCTT CAAGGTCTAC 
TGCAACTTCA CGGCCGGGGG CTCGACGTGC GTCTTCCCCG ACAAGAAGTC CGAGGGGGCC 
AGAATCACTT CTTGGCCCAA AGAAAACCCG GGTTCCTGGT TCAGTGAGTT CAAGCGTGGC 
AAACTGCTCT CCTACGTGGA CGCCGAGGGC AACCCCGTGG GCGTGGTCCA GATGACCTTC 
CTGCGGCTGC TCAGCGCCTC CGCCCACCAG AACATCACCT ACAACTGTTA CCAGTCGGTG 
GCCTGGCAGG ACGCCGCCAC AGGCAGCTAC GACAAGGCCA TGCGCTTCCT GGGCTCCAAC 
GACGAGGAGA TGTCGTATGA CAACAGCCCC TACATCCGCG CCCTGGTGGA CGGCTGCGCC 
ACGAAGAAGG GGTATCAGAA GACGGTTCTG GAGATCGACA CGCCCAAGGT GGAGCAGGTG 
CCCATCGTCG ACATTATGTT CAACGACTTC GGGGAAGCGT CACAGAAATT CGGATTTGAA 
GTGGGGCCGG CTTGCTTCCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011973343.1
CDS	39..5585
Translation	MDFHTRWKARSPLRPGAPLLSPLPPPLLLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCAARRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLEMGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSIHKKNVTLILDCKKKTTKFLDRSEHPVIDVNGIVVFGTRILDEEVFEGDIQQLLLVSDHRAAYDYCEHYSPDCDTAVPDKPQSQDPNPDEYYPDGDNEGDTYYYEYPYYEDTDDTGKDPVPTKTPVEAARETTEVPEELTQPPTEAPSAPSTTEGPGKEEDPGIGDYDYVPSEDYYTPPPYEDFGYGEGIENPDENPDQHPDQGARAEVPTSTVLTSNGSNPAPPPEEGMDDLEGEFTEETIKNLDESYYDPYYDPTISPSEVGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLLEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGEDGERGDDGEAGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGAPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGERGVPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGQPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLMDDAEGEQYMDYADGMEEIFGSLNSLKLEIEQMKRPLGTPQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNITYNCYQSVAWQDAATGSYDKAMRFLGSNDEEMSYDNSPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_012117953.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon collagen, type V, alpha 1 (COL5A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012117953.2

ORF Insert Sequence:

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGTGGGCGCC GCCTCCGAGC 
CGCGCAGCTC AGCCAGCAGA TCTCCTGAAG GTTCTAGATT TTCACAACCT GCCTGATGGA 
ATAACCAAGA CCACAGGCTT CTGTGCAGCG CGGCGATCTT CCAAAGGCCC GGATGTCGCC 
TACAGGGTCA CGAAAGATGC CCAGCTCAGC GCGCCCACCA AGCAGCTGTA CCCTGCATCT 
GCGTTTCCTG AGGACTTCTC CATCCTAACC ACTGTGAAAG CCAAGAAAGG CAGCCAGGCC 
TTCCTGGTCT CCATCTATAA CGAGCAGGGC ATCCAGCAGA TCGGCCTGGA GATGGGACGC 
TCCCCGGTCT TCCTCTACGA GGACCACACG GGGAAGCCGG GGCCAGAGGA CTACCCCCTC 
TTCAGAGGCA TCAACCTGTC GGATGGCAAG TGGCACAGAA TCGCTCTCAG CATCCACAAG 
AAGAATGTCA CCTTGATCCT GGACTGTAAA AAGAAGACGA CCAAGTTCCT GGACCGCAGT 
GAACACCCTG TGATAGACGT CAACGGCATC GTTGTATTCG GCACCCGGAT TCTGGATGAG 
GAAGTGTTTG AGGGTGACAT CCAGCAGCTG CTCCTCGTCT CGGACCACCG GGCGGCTTAC 
GATTACTGTG AGCATTACAG CCCAGACTGC GACACGGCCG TCCCTGACAA ACCCCAGTCT 
CAGGACCCCA ACCCAGACGA ATACTACCCA GATGGGGACA ATGAAGGAGA CACCTATTAC 
TACGAGTACC CCTACTACGA GGACACGGAC GACACGGGCA AGGACCCCGT CCCCACCAAG 
ACGCCCGTGG AAGCTGCTCG GGAGACCACG GAGGTGCCCG AGGAGCTGAC CCAGCCCCCA 
ACAGAAGCCC CTTCGGCGCC CAGCACCACG GAGGGCCCTG GGAAGGAGGA GGACCCCGGC 
ATCGGCGACT ATGACTACGT GCCCAGTGAG GACTACTACA CGCCACCCCC TTACGAGGAC 
TTCGGCTACG GTGAAGGCAT CGAGAACCCG GATGAGAACC CCGACCAGCA CCCGGACCAG 
GGCGCCCGGG CTGAGGTTCC CACCAGCACG GTCCTCACCT CCAACGGCTC CAACCCGGCC 
CCGCCTCCGG AGGAGGGGAT GGACGACCTG GAGGGAGAGT TCACGGAGGA AACCATCAAG 
AACCTGGACG AGAGCTACTA CGACCCGTAC TACGACCCCA CCATCTCCCC ATCGGAGGTC 
GGGCCAGGCA TGCCCGCCAA CCAGGACACC ATCTATGAAG GGATCGGAGG CCCTCGGGGC 
GAGAAAGGCC AGAAAGGAGA GCCTGCCATC ATCGAGCCGG GCATGCTCCT GGAGGGGCCG 
CCTGGCCCCG AAGGCCCTGC GGGTCTCCCA GGACCTCCAG GGACGATGGG TCCCACAGGC 
CAAGTGGGTG ACCCTGGAGA AAGGGGTCCC CCTGGACGCC CAGGCCTTCC TGGCGCTGAC 
GGGCTGCCTG GCCCTCCTGG AACCATGCTC ATGCTGCCTT TCCGGTTCGG AGGGGGCGGT 
GACGCGGGCT CCAAAGGACC CATGGTCTCC GCGCAGGAGT CCCAAGCTCA AGCCATCCTG 
CAGCAGGCCC GGCTCGCGTT GAGGGGGCCT GCTGGCCCGA TGGGTCTCAC TGGGAGACCG 
GGTCCCATGG GCCCTCCTGG GAGCGGAGGT CTGAAGGGCG AGCCAGGAGA CATGGGGCCT 
CAGGGTCCAC GGGGTGTGCA GGGCCCGCCT GGCCCGGCAG GAAAGCCTGG GAGAAGGGGC 
CGAGCCGGCA GTGACGGAGC GAGAGGGATG CCCGGGCAGA CTGGCCCTAA GGGTGACCGC 
GGCTTTGATG GCCTGGCGGG GCTGCCAGGG GAGAAAGGCC ACCGGGGTGA CCCTGGCCCA 
TCCGGCCCGC CGGGACCTCC AGGGGAAGAC GGAGAAAGGG GCGACGATGG AGAAGCTGGG 
CCCAGAGGGC TGCCTGGGGA GCCCGGGCCG CGAGGTCTGC TGGGGCCGAA GGGCCCCCCA 
GGCCCCCCTG GACCTCCCGG TGTGACAGGA ATGGATGGTC AACCAGGCCC GAAAGGCAAC 
GTGGGTCCCC AGGGAGAGCC CGGCCCCCCG GGGCAGCAGG GCAACCCAGG TGCCCAGGGT 
CTTCCGGGCC CCCAGGGCGC AATCGGGCCT CCAGGAGAAA AGGGTCCACT GGGCAAACCC 
GGCCTCCCAG GAATGCCCGG TGCTGATGGA CCCCCGGGGC ACCCTGGCAA AGAAGGCCCT 
CCAGGAGAGA AGGGAGGGCA GGGTCCACCT GGCCCCCAGG GTCCAATTGG CTACCCAGGT 
CCTCGCGGAG TCAAGGGGGC GGACGGCATC CGAGGTCTGA AGGGCACCAA GGGCGAGAAG 
GGTGAAGATG GCTTCCCGGG CTTCAAAGGA GACATGGGCA TCAAGGGCGA TCGGGGGGAG 
ATCGGCCCAC CTGGTCCCAG AGGGGAGGAC GGTCCCGAAG GTCCCAAGGG ACGAGGCGGT 
CCGAACGGCG ACCCCGGTCC TCTGGGACCC CCTGGGGAGA AGGGCAAACT CGGAGTCCCA 
GGACTGCCAG GCTACCCAGG AAGACAGGGG CCAAAGGGTT CTATCGGATT TCCTGGATTT 
CCTGGCGCCA ACGGAGAAAA GGGCGGCAGG GGCACCCCTG GGAAGCCGGG ACCGCGGGGG 
CAGCGAGGCC CGACGGGCCC ACGAGGTGAA AGAGGCCCAC GGGGCATCAC CGGGAAGCCT 
GGCCCCAAGG GCAACTCTGG AGGCGATGGC CCGGCTGGCC CTCCCGGTGA ACGGGGACCC 
AACGGACCCC AAGGACCCAC GGGGTTCCCT GGACCAAAGG GCCCCCCGGG GCCCCCAGGC 
AAGGACGGGC TCCCGGGACA CCCTGGACAG CGAGGCGAGA CGGGTTTCCA AGGCAAGACC 
GGCCCTCCAG GCCCTCCAGG TGTGGTCGGC CCTCAGGGCC CCACTGGGGA AACCGGTCCC 
ATGGGCGAGC GTGGTCACCC TGGCCCCCCA GGGCCCCCCG GTGAACAGGG GCTCCCGGGC 
CTGGCCGGGA AAGAAGGGAC GAAGGGAGAC CCAGGCCCCG CCGGCCTTCC TGGGAAAGAC 
GGTCCCCCTG GGCTGCGAGG CTTCCCTGGA GACCGAGGAC TTCCTGGTCC AGTGGGGGCG 
CTTGGACTGA AAGGCAGTGA AGGCCCCCCC GGCCCGCCAG GCCCCGCGGG ATCTCCAGGG 
GAGAGAGGTC CTGCCGGTGC CGCCGGGCCC ATTGGAATTC CAGGGAGGCC CGGGCCCCAG 
GGGCCCCCTG GGCCAGCTGG GGAGAAAGGC GCCCCTGGCG AGAAGGGTCC ACAAGGCCCA 
GCTGGCCGAG ATGGTCTCCA GGGCCCCGTG GGGCTCCCCG GTCCCGCTGG CCCTGTGGGC 
CCCCCTGGAG AAGACGGAGA CAAGGGAGAG ATTGGAGAGC CAGGACAGAA GGGCAGCAAG 
GGAGACAAAG GCGAGCAGGG TCCACCAGGG CCTACAGGAC CTCAAGGCCC CATCGGACAG 
CCAGGCCCCT CCGGAGCCGA TGGCGAACCG GGACCTCGGG GCCAGCAGGG CCTCTTTGGG 
CAGAAAGGCG ACGAAGGTCC GAGAGGCTTC CCCGGGCCCC CGGGTCCTGT GGGGCTGCAG 
GGTCTGCCGG GACCTCCTGG AGAGAAGGGC GAGACGGGTG ACGTGGGTCA GATGGGCCCG 
CCAGGTCCCC CTGGCCCCCG AGGACCCTCC GGAGCTCCAG GTGCAGATGG GCCACAAGGA 
CCCCCCGGTG GAATAGGCAA CCCCGGAGCC GTGGGAGAGA AGGGTGAGCC TGGCGAAGCG 
GGAGAGCCTG GCCTTCCCGG TGAAGGGGGC CCCCCGGGAC CCAAAGGCGA AAGGGGGGAG 
AAGGGCGAGT CGGGCCCCTC CGGTGCCGCC GGACCCCCGG GGCCCAAGGG CCCTCCCGGA 
GACGACGGTC CCAAAGGCAG CCCCGGCCCC GTCGGTTTTC CCGGAGATCC TGGTCCCCCC 
GGAGAACCTG GCCCCGCGGG TCAAGACGGT CCTCCTGGCG ACAAAGGAGA CGACGGTGAA 
CCCGGGCAAA CGGGATCACC AGGCCCTACG GGTGAGCCAG GCCCATCTGG GCCTCCAGGA 
AAAAGGGGTC CGCCCGGCCC CGCAGGCCCC GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG 
GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG AAGACTGGCC CCATCGGCCC CCAGGGGGCC 
CCCGGGAAGC CGGGGCCTGA CGGCCTTCGA GGGATCCCCG GCCCAGTGGG TGAACAAGGT 
CTCCCCGGAG CCCCGGGCCC CGATGGCCCC CCAGGACCCA TGGGCCCCCC AGGACTCCCC 
GGCCTCAAAG GAGACTCTGG GCCCAAAGGA GAAAAGGGTC ACCCAGGCTT GATCGGCCTC 
ATCGGACCTC CGGGCGAACA GGGAGAAAAA GGTGAACGTG GAGTCCCCGG ACCCCAGGGC 
TCCTCGGGCC CTAAGGGAGA ACAGGGTATC ACTGGTCCTT CCGGCCCGAT TGGCCCCCCA 
GGTCCCCCCG GCCTGCCGGG CCCCCCTGGT CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA 
ACTGGCCCGA AGGGTGAGGC AGGCCAGCCA GGACCCCCTG GCCCACCAGG CCCCCCGGGC 
GAGGTCATCC AGCCCCTGCC GATCCAAGCG TCCAGGACCC GGCGGAACAT CGACGCCAGC 
CAGCTGATGG ACGACGCAGA GGGCGAGCAG TACATGGACT ATGCGGACGG CATGGAGGAG 
ATCTTCGGCT CGCTCAACTC CCTGAAGCTG GAGATCGAGC AGATGAAGCG GCCGCTGGGC 
ACGCCGCAGA ACCCCGCGCG CACCTGCAAA GACCTCCAGC TCTGCCACCC CGACTTCCCC 
GATGGCGAAT ACTGGGTGGA TCCGAACCAA GGCTGCTCCC GGGACTCCTT CAAGGTCTAC 
TGCAACTTCA CGGCCGGGGG CTCGACGTGC GTCTTCCCCG ACAAGAAGTC CGAGGGGGCC 
AGAATCACTT CTTGGCCCAA AGAAAACCCG GGTTCCTGGT TCAGTGAGTT CAAGCGTGGC 
AAACTGCTCT CCTACGTGGA CGCCGAGGGC AACCCCGTGG GCGTGGTCCA GATGACCTTC 
CTGCGGCTGC TCAGCGCCTC CGCCCACCAG AACATCACCT ACAACTGTTA CCAGTCGGTG 
GCCTGGCAGG ACGCCGCCAC AGGCAGCTAC GACAAGGCCA TGCGCTTCCT GGGCTCCAAC 
GACGAGGAGA TGTCGTATGA CAACAGCCCC TACATCCGCG CCCTGGTGGA CGGCTGCGCC 
ACGAAGAAGG GGTATCAGAA GACGGTTCTG GAGATCGACA CGCCCAAGGT GGAGCAGGTG 
CCCATCGTCG ACATTATGTT CAACGACTTC GGGGAAGCGT CACAGAAATT CGGATTTGAA 
GTGGGGCCGG CTTGCTTCCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( XM_012117954.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011973344.1 Ovis aries musimon collagen, type V, alpha 1 (COL5A1), transcript variant X3, mRNA.

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CloneID	OOv05940
Clone ID Related Accession (Same CDS sequence)	XM_012117954.2
Accession Version	XM_012117954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	collagen alpha-1(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942270.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012117954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGTGGGCGCC GCCTCCGAGC 
CGCGCAGCTC AGCCAGCAGA TCTCCTGAAG GTTCTAGATT TTCACAACCT GCCTGATGGA 
ATAACCAAGA CCACAGGCTT CTGTGCAGCG CGGCGATCTT CCAAAGGCCC GGATGTCGCC 
TACAGGGTCA CGAAAGATGC CCAGCTCAGC GCGCCCACCA AGCAGCTGTA CCCTGCATCT 
GCGTTTCCTG AGGACTTCTC CATCCTAACC ACTGTGAAAG CCAAGAAAGG CAGCCAGGCC 
TTCCTGGTCT CCATCTATAA CGAGCAGGGC ATCCAGCAGA TCGGCCTGGA GATGGGACGC 
TCCCCGGTCT TCCTCTACGA GGACCACACG GGGAAGCCGG GGCCAGAGGA CTACCCCCTC 
TTCAGAGGCA TCAACCTGTC GGATGGCAAG TGGCACAGAA TCGCTCTCAG CATCCACAAG 
AAGAATGTCA CCTTGATCCT GGACTGTAAA AAGAAGACGA CCAAGTTCCT GGACCGCAGT 
GAACACCCTG TGATAGACGT CAACGGCATC GTTGTATTCG GCACCCGGAT TCTGGATGAG 
GAAGTGTTTG AGGGTGACAT CCAGCAGCTG CTCCTCGTCT CGGACCACCG GGCGGCTTAC 
GATTACTGTG AGCATTACAG CCCAGACTGC GACACGGCCG TCCCTGACAA ACCCCAGTCT 
CAGGACCCCA ACCCAGACGA ATACTACCCA GATGGGGACA ATGAAGGAGA CACCTATTAC 
TACGAGTACC CCTACTACGA GGACACGGAC GACACGGGCA AGGACCCCGT CCCCACCAAG 
ACGCCCGTGG AAGCTGCTCG GGAGACCACG GAGGTGCCCG AGGAGCTGAC CCAGCCCCCA 
ACAGAAGCCC CTTCGGCGCC CAGCACCACG GAGGGCCCTG GGAAGGAGGA GGACCCCGGC 
ATCGGCGACT ATGACTACGT GCCCAGTGAG GACTACTACA CGCCACCCCC TTACGAGGAC 
TTCGGCTACG GTGAAGGCAT CGAGAACCCG GATGAGAACC CCGACCAGCA CCCGGACCAG 
GGCGCCCGGG CTGAGGTTCC CACCAGCACG GTCCTCACCT CCAACGGCTC CAACCCGGCC 
CCGCCTCCGG AGGAGGGGAT GGACGACCTG GAGGGAGAGT TCACGGAGGA AACCATCAAG 
AACCTGGACG AGAGCTACTA CGACCCGTAC TACGACCCCA CCATCTCCCC ATCGGAGGTC 
GGGCCAGGCA TGCCCGCCAA CCAGGACACC ATCTATGAAG GGATCGGAGG CCCTCGGGGC 
GAGAAAGGCC AGAAAGGAGA GCCTGCCATC ATCGAGCCGG GCATGCTCCT GGAGGGGCCG 
CCTGGCCCCG AAGGCCCTGC GGGTCTCCCA GGACCTCCAG GGACGATGGG TCCCACAGGC 
CAAGTGGGTG ACCCTGGAGA AAGGGGTCCC CCTGGACGCC CAGGCCTTCC TGGCGCTGAC 
GGGCTGCCTG GCCCTCCTGG AACCATGCTC ATGCTGCCTT TCCGGTTCGG AGGGGGCGGT 
GACGCGGGCT CCAAAGGACC CATGGTCTCC GCGCAGGAGT CCCAAGCTCA AGCCATCCTG 
CAGCAGGCCC GGCTCGCGTT GAGGGGGCCT GCTGGCCCGA TGGGTCTCAC TGGGAGACCG 
GGTCCCATGG GCCCTCCTGG GAGCGGAGGT CTGAAGGGCG AGCCAGGAGA CATGGGGCCT 
CAGGGTCCAC GGGGTGTGCA GGGCCCGCCT GGCCCGGCAG GAAAGCCTGG GAGAAGGGGC 
CGAGCCGGCA GTGACGGAGC GAGAGGGATG CCCGGGCAGA CTGGCCCTAA GGGTGACCGC 
GGCTTTGATG GCCTGGCGGG GCTGCCAGGG GAGAAAGGCC ACCGGGGTGA CCCTGGCCCA 
TCCGGCCCGC CGGGACCTCC AGGGGAAGAC GGAGAAAGGG GCGACGATGG AGAAGCTGGG 
CCCAGAGGGC TGCCTGGGGA GCCCGGGCCG CGAGGTCTGC TGGGGCCGAA GGGCCCCCCA 
GGCCCCCCTG GACCTCCCGG TGTGACAGGA ATGGATGGTC AACCAGGCCC GAAAGGCAAC 
GTGGGTCCCC AGGGAGAGCC CGGCCCCCCG GGGCAGCAGG GCAACCCAGG TGCCCAGGGT 
CTTCCGGGCC CCCAGGGCGC AATCGGGCCT CCAGGAGAAA AGGGTCCACT GGGCAAACCC 
GGCCTCCCAG GAATGCCCGG TGCTGATGGA CCCCCGGGGC ACCCTGGCAA AGAAGGCCCT 
CCAGGAGAGA AGGGAGGGCA GGGTCCACCT GGCCCCCAGG GTCCAATTGG CTACCCAGGT 
CCTCGCGGAG TCAAGGGGGC GGACGGCATC CGAGGTCTGA AGGGCACCAA GGGCGAGAAG 
GGTGAAGATG GCTTCCCGGG CTTCAAAGGA GACATGGGCA TCAAGGGCGA TCGGGGGGAG 
ATCGGCCCAC CTGGTCCCAG AGGGGAGGAC GGTCCCGAAG GTCCCAAGGG ACGAGGCGGT 
CCGAACGGCG ACCCCGGTCC TCTGGGACCC CCTGGGGAGA AGGGCAAACT CGGAGTCCCA 
GGACTGCCAG GCTACCCAGG AAGACAGGGG CCAAAGGGTT CTATCGGATT TCCTGGATTT 
CCTGGCGCCA ACGGAGAAAA GGGCGGCAGG GGCACCCCTG GGAAGCCGGG ACCGCGGGGG 
CAGCGAGGCC CGACGGGCCC ACGAGGTGAA AGAGGCCCAC GGGGCATCAC CGGGAAGCCT 
GGCCCCAAGG GCAACTCTGG AGGCGATGGC CCGGCTGGCC CTCCCGGTGA ACGGGGACCC 
AACGGACCCC AAGGACCCAC GGGGTTCCCT GGACCAAAGG GCCCCCCGGG GCCCCCAGGC 
AAGGACGGGC TCCCGGGACA CCCTGGACAG CGAGGCGAGA CGGGTTTCCA AGGCAAGACC 
GGCCCTCCAG GCCCTCCAGG TGTGGTCGGC CCTCAGGGCC CCACTGGGGA AACCGGTCCC 
ATGGGCGAGC GTGGTCACCC TGGCCCCCCA GGGCCCCCCG GTGAACAGGG GCTCCCGGGC 
CTGGCCGGGA AAGAAGGGAC GAAGGGAGAC CCAGGCCCCG CCGGCCTTCC TGGGAAAGAC 
GGTCCCCCTG GGCTGCGAGG CTTCCCTGGA GACCGAGGAC TTCCTGGTCC AGTGGGGGCG 
CTTGGACTGA AAGGCAGTGA AGGCCCCCCC GGCCCGCCAG GCCCCGCGGG ATCTCCAGGG 
GAGAGAGGTC CTGCCGGTGC CGCCGGGCCC ATTGGAATTC CAGGGAGGCC CGGGCCCCAG 
GGGCCCCCTG GGCCAGCTGG GGAGAAAGGC GCCCCTGGCG AGAAGGGTCC ACAAGGCCCA 
GCTGGCCGAG ATGGTCTCCA GGGCCCCGTG GGGCTCCCCG GTCCCGCTGG CCCTGTGGGC 
CCCCCTGGAG AAGACGGAGA CAAGGGAGAG ATTGGAGAGC CAGGACAGAA GGGCAGCAAG 
GGAGACAAAG GCGAGCAGGG TCCACCAGGG CCTACAGGAC CTCAAGGCCC CATCGGACAG 
CCAGGCCCCT CCGGAGCCGA TGGCGAACCG GGACCTCGGG GCCAGCAGGG CCTCTTTGGG 
CAGAAAGGCG ACGAAGGTCC GAGAGGCTTC CCCGGGCCCC CGGGTCCTGT GGGGCTGCAG 
GGTCTGCCGG GACCTCCTGG AGAGAAGGGC GAGACGGGTG ACGTGGGTCA GATGGGCCCG 
CCAGGTCCCC CTGGCCCCCG AGGACCCTCC GGAGCTCCAG GTGCAGATGG GCCACAAGGA 
CCCCCCGGTG GAATAGGCAA CCCCGGAGCC GTGGGAGAGA AGGGTGAGCC TGGCGAAGCG 
GGAGAGCCTG GCCTTCCCGG TGAAGGGGGC CCCCCGGGAC CCAAAGGCGA AAGGGGGGAG 
AAGGGCGAGT CGGGCCCCTC CGGTGCCGCC GGACCCCCGG GGCCCAAGGG CCCTCCCGGA 
GACGACGGTC CCAAAGGCAG CCCCGGCCCC GTCGGTTTTC CCGGAGATCC TGGTCCCCCC 
GGAGAACCTG GCCCCGCGGG TCAAGACGGT CCTCCTGGCG ACAAAGGAGA CGACGGTGAA 
CCCGGGCAAA CGGGATCACC AGGCCCTACG GGTGAGCCAG GCCCATCTGG GCCTCCAGGA 
AAAAGGGGTC CGCCCGGCCC CGCAGGCCCC GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG 
GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG AAGACTGGCC CCATCGGCCC CCAGGGGGCC 
CCCGGGAAGC CGGGGCCTGA CGGCCTTCGA GGGATCCCCG GCCCAGTGGG TGAACAAGGT 
CTCCCCGGAG CCCCGGGCCC CGATGGCCCC CCAGGACCCA TGGGCCCCCC AGGACTCCCC 
GGCCTCAAAG GAGACTCTGG GCCCAAAGGA GAAAAGGGTC ACCCAGGCTT GATCGGCCTC 
ATCGGACCTC CGGGCGAACA GGGAGAAAAA GGTGAACGTG GAGTCCCCGG ACCCCAGGGC 
TCCTCGGGCC CTAAGGGAGA ACAGGGTATC ACTGGTCCTT CCGGCCCGAT TGGCCCCCCA 
GGTCCCCCCG GCCTGCCGGG CCCCCCTGGT CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA 
ACTGGCCCGA AGGGTGAGGC AGGCCAGCCA GGACCCCCTG GCCCACCAGG CCCCCCGGGC 
GAGGTCATCC AGCCCCTGCC GATCCAAGCG TCCAGGACCC GGCGGAACAT CGACGCCAGC 
CAGCTGATGG ACGACGCAGA GGGCGAGCAG TACATGGACT ATGCGGACGG CATGGAGGAG 
ATCTTCGGCT CGCTCAACTC CCTGAAGCTG GAGATCGAGC AGATGAAGCG GCCGCTGGGC 
ACGCCGCAGA ACCCCGCGCG CACCTGCAAA GACCTCCAGC TCTGCCACCC CGACTTCCCC 
GATGGCGAAT ACTGGGTGGA TCCGAACCAA GGCTGCTCCC GGGACTCCTT CAAGGTCTAC 
TGCAACTTCA CGGCCGGGGG CTCGACGTGC GTCTTCCCCG ACAAGAAGTC CGAGGGGAGT 
AAGATGGCCC GCTGGCCCAA AGAGCAGCCC TCCACCTGGT ACAGTCAGTA CAAGCGGGGG 
TCGCTGCTCT CCTACGTGGA CGCCGAGGGC AACCCCGTGG GCGTGGTCCA GATGACCTTC 
CTGCGGCTGC TCAGCGCCTC CGCCCACCAG AACATCACCT ACAACTGTTA CCAGTCGGTG 
GCCTGGCAGG ACGCCGCCAC AGGCAGCTAC GACAAGGCCA TGCGCTTCCT GGGCTCCAAC 
GACGAGGAGA TGTCGTATGA CAACAGCCCC TACATCCGCG CCCTGGTGGA CGGCTGCGCC 
ACGAAGAAGG GGTATCAGAA GACGGTTCTG GAGATCGACA CGCCCAAGGT GGAGCAGGTG 
CCCATCGTCG ACATTATGTT CAACGACTTC GGGGAAGCGT CACAGAAATT CGGATTTGAA 
GTGGGGCCGG CTTGCTTCCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011973344.1
CDS	39..5585
Translation	MDFHTRWKARSPLRPGAPLLSPLPPPLLLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCAARRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLEMGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSIHKKNVTLILDCKKKTTKFLDRSEHPVIDVNGIVVFGTRILDEEVFEGDIQQLLLVSDHRAAYDYCEHYSPDCDTAVPDKPQSQDPNPDEYYPDGDNEGDTYYYEYPYYEDTDDTGKDPVPTKTPVEAARETTEVPEELTQPPTEAPSAPSTTEGPGKEEDPGIGDYDYVPSEDYYTPPPYEDFGYGEGIENPDENPDQHPDQGARAEVPTSTVLTSNGSNPAPPPEEGMDDLEGEFTEETIKNLDESYYDPYYDPTISPSEVGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLLEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGEDGERGDDGEAGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGAPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGERGVPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGQPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLMDDAEGEQYMDYADGMEEIFGSLNSLKLEIEQMKRPLGTPQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGVVQMTFLRLLSASAHQNITYNCYQSVAWQDAATGSYDKAMRFLGSNDEEMSYDNSPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_012117954.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon collagen, type V, alpha 1 (COL5A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012117954.2

ORF Insert Sequence:

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGTGGGCGCC GCCTCCGAGC 
CGCGCAGCTC AGCCAGCAGA TCTCCTGAAG GTTCTAGATT TTCACAACCT GCCTGATGGA 
ATAACCAAGA CCACAGGCTT CTGTGCAGCG CGGCGATCTT CCAAAGGCCC GGATGTCGCC 
TACAGGGTCA CGAAAGATGC CCAGCTCAGC GCGCCCACCA AGCAGCTGTA CCCTGCATCT 
GCGTTTCCTG AGGACTTCTC CATCCTAACC ACTGTGAAAG CCAAGAAAGG CAGCCAGGCC 
TTCCTGGTCT CCATCTATAA CGAGCAGGGC ATCCAGCAGA TCGGCCTGGA GATGGGACGC 
TCCCCGGTCT TCCTCTACGA GGACCACACG GGGAAGCCGG GGCCAGAGGA CTACCCCCTC 
TTCAGAGGCA TCAACCTGTC GGATGGCAAG TGGCACAGAA TCGCTCTCAG CATCCACAAG 
AAGAATGTCA CCTTGATCCT GGACTGTAAA AAGAAGACGA CCAAGTTCCT GGACCGCAGT 
GAACACCCTG TGATAGACGT CAACGGCATC GTTGTATTCG GCACCCGGAT TCTGGATGAG 
GAAGTGTTTG AGGGTGACAT CCAGCAGCTG CTCCTCGTCT CGGACCACCG GGCGGCTTAC 
GATTACTGTG AGCATTACAG CCCAGACTGC GACACGGCCG TCCCTGACAA ACCCCAGTCT 
CAGGACCCCA ACCCAGACGA ATACTACCCA GATGGGGACA ATGAAGGAGA CACCTATTAC 
TACGAGTACC CCTACTACGA GGACACGGAC GACACGGGCA AGGACCCCGT CCCCACCAAG 
ACGCCCGTGG AAGCTGCTCG GGAGACCACG GAGGTGCCCG AGGAGCTGAC CCAGCCCCCA 
ACAGAAGCCC CTTCGGCGCC CAGCACCACG GAGGGCCCTG GGAAGGAGGA GGACCCCGGC 
ATCGGCGACT ATGACTACGT GCCCAGTGAG GACTACTACA CGCCACCCCC TTACGAGGAC 
TTCGGCTACG GTGAAGGCAT CGAGAACCCG GATGAGAACC CCGACCAGCA CCCGGACCAG 
GGCGCCCGGG CTGAGGTTCC CACCAGCACG GTCCTCACCT CCAACGGCTC CAACCCGGCC 
CCGCCTCCGG AGGAGGGGAT GGACGACCTG GAGGGAGAGT TCACGGAGGA AACCATCAAG 
AACCTGGACG AGAGCTACTA CGACCCGTAC TACGACCCCA CCATCTCCCC ATCGGAGGTC 
GGGCCAGGCA TGCCCGCCAA CCAGGACACC ATCTATGAAG GGATCGGAGG CCCTCGGGGC 
GAGAAAGGCC AGAAAGGAGA GCCTGCCATC ATCGAGCCGG GCATGCTCCT GGAGGGGCCG 
CCTGGCCCCG AAGGCCCTGC GGGTCTCCCA GGACCTCCAG GGACGATGGG TCCCACAGGC 
CAAGTGGGTG ACCCTGGAGA AAGGGGTCCC CCTGGACGCC CAGGCCTTCC TGGCGCTGAC 
GGGCTGCCTG GCCCTCCTGG AACCATGCTC ATGCTGCCTT TCCGGTTCGG AGGGGGCGGT 
GACGCGGGCT CCAAAGGACC CATGGTCTCC GCGCAGGAGT CCCAAGCTCA AGCCATCCTG 
CAGCAGGCCC GGCTCGCGTT GAGGGGGCCT GCTGGCCCGA TGGGTCTCAC TGGGAGACCG 
GGTCCCATGG GCCCTCCTGG GAGCGGAGGT CTGAAGGGCG AGCCAGGAGA CATGGGGCCT 
CAGGGTCCAC GGGGTGTGCA GGGCCCGCCT GGCCCGGCAG GAAAGCCTGG GAGAAGGGGC 
CGAGCCGGCA GTGACGGAGC GAGAGGGATG CCCGGGCAGA CTGGCCCTAA GGGTGACCGC 
GGCTTTGATG GCCTGGCGGG GCTGCCAGGG GAGAAAGGCC ACCGGGGTGA CCCTGGCCCA 
TCCGGCCCGC CGGGACCTCC AGGGGAAGAC GGAGAAAGGG GCGACGATGG AGAAGCTGGG 
CCCAGAGGGC TGCCTGGGGA GCCCGGGCCG CGAGGTCTGC TGGGGCCGAA GGGCCCCCCA 
GGCCCCCCTG GACCTCCCGG TGTGACAGGA ATGGATGGTC AACCAGGCCC GAAAGGCAAC 
GTGGGTCCCC AGGGAGAGCC CGGCCCCCCG GGGCAGCAGG GCAACCCAGG TGCCCAGGGT 
CTTCCGGGCC CCCAGGGCGC AATCGGGCCT CCAGGAGAAA AGGGTCCACT GGGCAAACCC 
GGCCTCCCAG GAATGCCCGG TGCTGATGGA CCCCCGGGGC ACCCTGGCAA AGAAGGCCCT 
CCAGGAGAGA AGGGAGGGCA GGGTCCACCT GGCCCCCAGG GTCCAATTGG CTACCCAGGT 
CCTCGCGGAG TCAAGGGGGC GGACGGCATC CGAGGTCTGA AGGGCACCAA GGGCGAGAAG 
GGTGAAGATG GCTTCCCGGG CTTCAAAGGA GACATGGGCA TCAAGGGCGA TCGGGGGGAG 
ATCGGCCCAC CTGGTCCCAG AGGGGAGGAC GGTCCCGAAG GTCCCAAGGG ACGAGGCGGT 
CCGAACGGCG ACCCCGGTCC TCTGGGACCC CCTGGGGAGA AGGGCAAACT CGGAGTCCCA 
GGACTGCCAG GCTACCCAGG AAGACAGGGG CCAAAGGGTT CTATCGGATT TCCTGGATTT 
CCTGGCGCCA ACGGAGAAAA GGGCGGCAGG GGCACCCCTG GGAAGCCGGG ACCGCGGGGG 
CAGCGAGGCC CGACGGGCCC ACGAGGTGAA AGAGGCCCAC GGGGCATCAC CGGGAAGCCT 
GGCCCCAAGG GCAACTCTGG AGGCGATGGC CCGGCTGGCC CTCCCGGTGA ACGGGGACCC 
AACGGACCCC AAGGACCCAC GGGGTTCCCT GGACCAAAGG GCCCCCCGGG GCCCCCAGGC 
AAGGACGGGC TCCCGGGACA CCCTGGACAG CGAGGCGAGA CGGGTTTCCA AGGCAAGACC 
GGCCCTCCAG GCCCTCCAGG TGTGGTCGGC CCTCAGGGCC CCACTGGGGA AACCGGTCCC 
ATGGGCGAGC GTGGTCACCC TGGCCCCCCA GGGCCCCCCG GTGAACAGGG GCTCCCGGGC 
CTGGCCGGGA AAGAAGGGAC GAAGGGAGAC CCAGGCCCCG CCGGCCTTCC TGGGAAAGAC 
GGTCCCCCTG GGCTGCGAGG CTTCCCTGGA GACCGAGGAC TTCCTGGTCC AGTGGGGGCG 
CTTGGACTGA AAGGCAGTGA AGGCCCCCCC GGCCCGCCAG GCCCCGCGGG ATCTCCAGGG 
GAGAGAGGTC CTGCCGGTGC CGCCGGGCCC ATTGGAATTC CAGGGAGGCC CGGGCCCCAG 
GGGCCCCCTG GGCCAGCTGG GGAGAAAGGC GCCCCTGGCG AGAAGGGTCC ACAAGGCCCA 
GCTGGCCGAG ATGGTCTCCA GGGCCCCGTG GGGCTCCCCG GTCCCGCTGG CCCTGTGGGC 
CCCCCTGGAG AAGACGGAGA CAAGGGAGAG ATTGGAGAGC CAGGACAGAA GGGCAGCAAG 
GGAGACAAAG GCGAGCAGGG TCCACCAGGG CCTACAGGAC CTCAAGGCCC CATCGGACAG 
CCAGGCCCCT CCGGAGCCGA TGGCGAACCG GGACCTCGGG GCCAGCAGGG CCTCTTTGGG 
CAGAAAGGCG ACGAAGGTCC GAGAGGCTTC CCCGGGCCCC CGGGTCCTGT GGGGCTGCAG 
GGTCTGCCGG GACCTCCTGG AGAGAAGGGC GAGACGGGTG ACGTGGGTCA GATGGGCCCG 
CCAGGTCCCC CTGGCCCCCG AGGACCCTCC GGAGCTCCAG GTGCAGATGG GCCACAAGGA 
CCCCCCGGTG GAATAGGCAA CCCCGGAGCC GTGGGAGAGA AGGGTGAGCC TGGCGAAGCG 
GGAGAGCCTG GCCTTCCCGG TGAAGGGGGC CCCCCGGGAC CCAAAGGCGA AAGGGGGGAG 
AAGGGCGAGT CGGGCCCCTC CGGTGCCGCC GGACCCCCGG GGCCCAAGGG CCCTCCCGGA 
GACGACGGTC CCAAAGGCAG CCCCGGCCCC GTCGGTTTTC CCGGAGATCC TGGTCCCCCC 
GGAGAACCTG GCCCCGCGGG TCAAGACGGT CCTCCTGGCG ACAAAGGAGA CGACGGTGAA 
CCCGGGCAAA CGGGATCACC AGGCCCTACG GGTGAGCCAG GCCCATCTGG GCCTCCAGGA 
AAAAGGGGTC CGCCCGGCCC CGCAGGCCCC GAAGGCAGGC AGGGGGAGAA AGGAGCCAAG 
GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG AAGACTGGCC CCATCGGCCC CCAGGGGGCC 
CCCGGGAAGC CGGGGCCTGA CGGCCTTCGA GGGATCCCCG GCCCAGTGGG TGAACAAGGT 
CTCCCCGGAG CCCCGGGCCC CGATGGCCCC CCAGGACCCA TGGGCCCCCC AGGACTCCCC 
GGCCTCAAAG GAGACTCTGG GCCCAAAGGA GAAAAGGGTC ACCCAGGCTT GATCGGCCTC 
ATCGGACCTC CGGGCGAACA GGGAGAAAAA GGTGAACGTG GAGTCCCCGG ACCCCAGGGC 
TCCTCGGGCC CTAAGGGAGA ACAGGGTATC ACTGGTCCTT CCGGCCCGAT TGGCCCCCCA 
GGTCCCCCCG GCCTGCCGGG CCCCCCTGGT CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA 
ACTGGCCCGA AGGGTGAGGC AGGCCAGCCA GGACCCCCTG GCCCACCAGG CCCCCCGGGC 
GAGGTCATCC AGCCCCTGCC GATCCAAGCG TCCAGGACCC GGCGGAACAT CGACGCCAGC 
CAGCTGATGG ACGACGCAGA GGGCGAGCAG TACATGGACT ATGCGGACGG CATGGAGGAG 
ATCTTCGGCT CGCTCAACTC CCTGAAGCTG GAGATCGAGC AGATGAAGCG GCCGCTGGGC 
ACGCCGCAGA ACCCCGCGCG CACCTGCAAA GACCTCCAGC TCTGCCACCC CGACTTCCCC 
GATGGCGAAT ACTGGGTGGA TCCGAACCAA GGCTGCTCCC GGGACTCCTT CAAGGTCTAC 
TGCAACTTCA CGGCCGGGGG CTCGACGTGC GTCTTCCCCG ACAAGAAGTC CGAGGGGAGT 
AAGATGGCCC GCTGGCCCAA AGAGCAGCCC TCCACCTGGT ACAGTCAGTA CAAGCGGGGG 
TCGCTGCTCT CCTACGTGGA CGCCGAGGGC AACCCCGTGG GCGTGGTCCA GATGACCTTC 
CTGCGGCTGC TCAGCGCCTC CGCCCACCAG AACATCACCT ACAACTGTTA CCAGTCGGTG 
GCCTGGCAGG ACGCCGCCAC AGGCAGCTAC GACAAGGCCA TGCGCTTCCT GGGCTCCAAC 
GACGAGGAGA TGTCGTATGA CAACAGCCCC TACATCCGCG CCCTGGTGGA CGGCTGCGCC 
ACGAAGAAGG GGTATCAGAA GACGGTTCTG GAGATCGACA CGCCCAAGGT GGAGCAGGTG 
CCCATCGTCG ACATTATGTT CAACGACTTC GGGGAAGCGT CACAGAAATT CGGATTTGAA 
GTGGGGCCGG CTTGCTTCCT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( XM_027966117.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027821918.1 Ovis aries collagen type V alpha 1 chain (COL5A1), transcript variant X1, mRNA.

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CloneID	OSm76411
Clone ID Related Accession (Same CDS sequence)	XM_027966117.1
Accession Version	XM_027966117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGCTGTGGGC GCCGCCTCCG 
AGCCGCGCAG CTCAGCCAGC AGATCTCCTG AAGGTTCTAG ATTTTCACAA CCTGCCTGAT 
GGAATAACCA AGACCACAGG CTTCTGTGCA GCGCGGCGAT CTTCCAAAGG CCCGGATGTC 
GCCTACAGAG TCACGAAAGA TGCCCAGCTC AGCGCGCCCA CCAAGCAGCT GTACCCTGCA 
TCTGCGTTTC CTGAGGACTT CTCCATCCTA ACCACTGTGA AAGCCAAGAA AGGCAGCCAG 
GCCTTCCTGG TCTCCATCTA TAACGAGCAG GGCATCCAGC AGATCGGCCT GGAGATGGGA 
CGCTCCCCGG TCTTCCTCTA CGAGGACCAC ACGGGGAAGC CGGGGCCAGA GGACTACCCC 
CTCTTCAGAG GCATCAACCT GTCGGATGGC AAGTGGCACA GAATCGCTCT CAGCATCCAC 
AAGAAGAATG TCACCTTGAT CCTGGACTGT AAAAAGAAGA CGACCAAGTT CCTGGACCGC 
AGCGAACACC CTGTGATAGA CGTCAACGGC ATCGTTGTAT TCGGCACCCG GATTCTGGAT 
GAAGAAGTGT TTGAGGGTGA CATCCAGCAG CTGCTCCTCG TCTCGGACCA CCGGGCGGCT 
TACGATTACT GTGAGCATTA CAGCCCAGAC TGCGACACGG CCGTCCCCGA CAAACCCCAG 
TCTCAGGACC CCAACCCAGA CGAATACTAC CCAGATGGGG ACAATGAAGG AGACACCTAT 
TACTACGAGT ACCCCTACTA CGAGGACACG GACGACACGG GCAAGGACCC CGTCCCCACC 
AAGACGCCCG TGGAAGCTGC TCGGGAGACC ACGGAGGTGC CCGAGGAGCT GACCCAGCCC 
CCAACAGAAG CCCCTTCAGC GCCCAGCACC ACGGAGGGCC CTGGGAAGGA GGAGGACCCC 
GGCATCGGCG ACTATGACTA CGTGCCCAGT GAGGACTACT ACACGCCACC CCCTTACGAG 
GACTTCGGCT ACGGTGAAGG CATCGAGAAC CCGGATGAGA ACCCTGACCA GCACCCGGAC 
CAGGGCGCCC GGGCTGAGGT TCCCACCAGC ACGGTCCTCA CCTCCAACGG CTCCAACCCG 
GCCCCGCCTC CGGAGGAGGG GATGGACGAC CTGGAGGGAG AGTTCACGGA GGAAACCATC 
AAGAACCTGG ACGAGAGCTA CTACGACCCG TACTACGACC CCACCATCTC CCCATCGGAG 
GTCGGGCCAG GCATGCCCGC CAACCAGGAC ACCATCTATG AAGGGATCGG AGGCCCTCGG 
GGCGAGAAAG GCCAGAAAGG AGAGCCCGCC ATCATCGAGC CGGGCATGCT CCTGGAGGGG 
CCGCCCGGCC CCGAAGGCCC TGCGGGTCTC CCAGGACCTC CAGGGACGAT GGGTCCCACA 
GGCCAAGTGG GTGACCCTGG AGAAAGGGGT CCCCCTGGAC GCCCAGGCCT TCCTGGCGCT 
GACGGGCTGC CTGGCCCTCC TGGAACCATG CTCATGCTGC CTTTCCGGTT CGGAGGGGGC 
GGTGACGCGG GCTCCAAAGG ACCCATGGTC TCCGCGCAGG AGTCCCAAGC TCAAGCCATC 
CTGCAGCAGG CCCGGCTCGC GTTGAGGGGG CCTGCTGGCC CGATGGGTCT CACTGGGAGA 
CCGGGTCCCA TGGGCCCTCC TGGGAGCGGA GGTCTGAAGG GCGAGCCAGG AGACATGGGG 
CCTCAGGGTC CACGGGGTGT GCAGGGCCCG CCTGGCCCGG CAGGAAAGCC TGGGAGAAGG 
GGCCGAGCCG GCAGTGACGG AGCGAGAGGG ATGCCCGGGC AGACTGGCCC CAAGGGTGAC 
CGCGGCTTTG ATGGCCTGGC GGGGCTGCCA GGGGAGAAAG GCCACCGGGG TGACCCTGGC 
CCATCCGGCC CGCCGGGACC TCCAGGGGAA GACGGAGAAA GGGGCGACGA TGGAGAAGCT 
GGGCCCAGAG GGCTGCCTGG GGAGCCCGGG CCGCGAGGTC TGCTGGGGCC GAAGGGCCCC 
CCAGGCCCCC CTGGACCTCC CGGTGTGACA GGAATGGATG GTCAACCAGG CCCGAAAGGC 
AACGTGGGTC CCCAGGGAGA GCCCGGCCCC CCGGGGCAGC AGGGCAACCC AGGTGCCCAG 
GGTCTTCCGG GCCCCCAGGG CGCAATCGGG CCTCCAGGAG AAAAGGGTCC ACTGGGCAAA 
CCCGGCCTCC CAGGAATGCC TGGTGCTGAT GGACCCCCGG GGCACCCTGG CAAAGAAGGC 
CCTCCAGGAG AGAAGGGAGG GCAGGGTCCA CCTGGCCCCC AGGGTCCAAT TGGCTACCCA 
GGTCCTCGCG GAGTCAAGGG GGCGGACGGC ATCCGAGGTC TGAAGGGCAC CAAGGGCGAG 
AAGGGTGAAG ATGGCTTCCC GGGCTTCAAA GGAGACATGG GCATCAAGGG CGATCGGGGG 
GAGATCGGCC CACCTGGTCC CAGAGGGGAG GACGGTCCCG AAGGTCCCAA GGGACGAGGC 
GGTCCGAACG GCGACCCTGG TCCTCTGGGA CCCCCTGGGG AGAAGGGCAA ACTCGGAGTC 
CCAGGACTGC CAGGCTACCC AGGAAGACAG GGGCCAAAGG GTTCTATCGG ATTTCCTGGA 
TTTCCTGGCG CCAACGGAGA AAAGGGCGGC AGGGGCACCC CTGGGAAGCC GGGACCGCGG 
GGGCAGCGAG GCCCGACGGG CCCACGAGGT GAAAGAGGCC CACGGGGCAT AACCGGGAAG 
CCTGGCCCCA AGGGCAACTC TGGAGGCGAT GGCCCGGCTG GCCCTCCCGG TGAACGGGGA 
CCCAACGGAC CCCAAGGACC CACGGGGTTC CCTGGACCAA AGGGCCCCCC GGGGCCCCCA 
GGCAAGGACG GGCTCCCGGG ACACCCTGGA CAGCGAGGCG AGACGGGTTT CCAAGGCAAG 
ACCGGCCCTC CAGGCCCTCC GGGTGTGGTC GGCCCTCAGG GTCCCACTGG GGAAACTGGT 
CCCATGGGCG AACGTGGCCA CCCTGGTCCC CCAGGGCCCC CCGGTGAACA GGGGCTCCCG 
GGCCTGGCCG GGAAAGAAGG GACGAAGGGA GACCCAGGCC CCGCCGGCCT TCCTGGGAAA 
GACGGTCCCC CCGGGCTGCG AGGCTTCCCT GGAGACCGAG GACTTCCTGG TCCAGTGGGG 
GCGCTTGGAC TGAAAGGCAG TGAAGGCCCC CCCGGCCCGC CAGGCCCCGC GGGATCTCCA 
GGGGAGAGAG GTCCTGCCGG TGCCGCCGGG CCCATTGGAA TTCCAGGGAG GCCCGGGCCC 
CAGGGGCCCC CTGGGCCAGC TGGGGAGAAA GGCGCCCCTG GCGAGAAGGG TCCACAAGGC 
CCAGCTGGCC GAGATGGTCT CCAGGGCCCC GTGGGGCTCC CCGGTCCCGC TGGCCCTGTG 
GGCCCCCCTG GAGAAGACGG AGACAAGGGA GAGATTGGAG AGCCAGGACA GAAGGGCAGC 
AAGGGAGACA AAGGCGAGCA GGGTCCACCA GGGCCTACAG GACCTCAAGG CCCCATCGGA 
CAGCCAGGCC CCTCCGGAGC TGATGGCGAA CCGGGACCTC GGGGCCAGCA GGGCCTCTTT 
GGGCAGAAAG GCGACGAAGG TCCGAGAGGC TTCCCCGGGC CCCCGGGTCC TGTGGGGCTG 
CAGGGTCTGC CGGGACCTCC TGGAGAGAAG GGCGAGACGG GTGACGTGGG TCAGATGGGC 
CCGCCAGGTC CCCCCGGCCC CCGAGGACCC TCCGGAGCTC CAGGTGCAGA TGGGCCACAA 
GGACCCCCCG GTGGAATAGG CAACCCCGGA GCCGTGGGAG AGAAGGGTGA GCCTGGCGAA 
GCGGGAGAGC CTGGCCTTCC CGGTGAAGGG GGCCCCCCGG GACCCAAAGG CGAAAGGGGG 
GAGAAGGGCG AGTCAGGCCC CTCCGGTGCC GCCGGACCCC CGGGGCCCAA GGGCCCTCCC 
GGAGACGACG GTCCCAAAGG CAGCCCCGGC CCCGTCGGTT TTCCCGGAGA TCCTGGTCCC 
CCCGGAGAAC CTGGCCCCGC GGGTCAAGAC GGTCCTCCTG GCGACAAAGG AGACGACGGT 
GAACCCGGGC AAACGGGATC ACCAGGCCCT ACGGGTGAGC CAGGCCCATC TGGGCCTCCA 
GGAAAAAGGG GTCCGCCCGG CCCCGCAGGC CCCGAAGGCA GGCAGGGGGA GAAAGGAGCC 
AAGGGAGAAG CCGGCTTGGA AGGCCCTCCT GGGAAGACTG GCCCCATCGG CCCCCAGGGG 
GCCCCCGGGA AGCCGGGGCC TGACGGCCTT CGAGGGATCC CCGGCCCAGT GGGTGAACAA 
GGTCTCCCTG GAGCCCCGGG CCCCGATGGC CCCCCAGGAC CCATGGGCCC CCCAGGACTC 
CCCGGCCTCA AAGGAGACTC TGGACCCAAA GGAGAAAAGG GTCACCCAGG CTTGATCGGC 
CTCATCGGAC CTCCGGGCGA ACAGGGAGAA AAGGGTGAAC GTGGAGTCCC CGGACCCCAG 
GGCTCCTCGG GCCCTAAGGG AGAACAGGGT ATCACTGGTC CTTCCGGCCC GATTGGCCCC 
CCAGGTCCCC CCGGCCTGCC GGGCCCCCCT GGTCCAAAAG GTGCTAAGGG CTCCTCGGGT 
CCAACTGGCC CGAAGGGTGA GGCAGGCCAG CCAGGACCCC CTGGCCCACC AGGCCCCCCG 
GGCGAGGTCA TCCAGCCCCT GCCGATCCAG GCGTCCAGGA CCCGGCGGAA CATCGACGCC 
AGCCAGCTGA TGGACGACGC AGAGGGCGAG CAGTACATGG ACTATGCGGA CGGCATGGAG 
GAGATCTTCG GCTCGCTCAA CTCCCTGAAG CTGGAGATCG AGCAGATGAA GCGGCCGCTG 
GGCACGCCGC AGAACCCCGC GCGCACCTGC AAAGACCTCC AGCTCTGCCA CCCCGACTTC 
CCCGATGGCG AATACTGGGT GGATCCGAAC CAAGGCTGCT CCCGGGACTC CTTCAAGGTG 
TACTGCAACT TCACGGCCGG GGGCTCGACG TGCGTCTTCC CCGACAAGAA GTCCGAGGGG 
GCCAGAATCA CTTCTTGGCC CAAAGAAAAC CCGGGTTCCT GGTTCAGTGA GTTCAAGCGT 
GGCAAACTGC TCTCCTACGT GGACGCCGAG GGCAACCCCG TGGGCGTGGT CCAGATGACC 
TTCCTGCGGC TGCTCAGCGC CTCCGCCCAC CAGAACATCA CCTACAACTG CTACCAGTCG 
GTGGCCTGGC AGGACGCCGC CACGGGCAGC TACGACAAGG CCATGCGCTT CCTGGGCTCC 
AACGACGAGG AGATGTCGTA TGACAACAGC CCCTACATCC GTGCCCTGGT GGACGGCTGC 
GCCACGAAGA AAGGGTATCA GAAGACGGTT CTGGAGATCG ACACGCCCAA GGTGGAGCAG 
GTGCCCATCG TCGACATTAT GTTCAACGAC TTCGGGGAAG CGTCACAGAA ATTCGGATTT 
GAAGTGGGGC CGGCTTGCTT CCTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027821918.1
CDS	409..5958
Translation	MDFHTRWKARSPLRPGAPLLSPLPPPLLLLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCAARRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLEMGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSIHKKNVTLILDCKKKTTKFLDRSEHPVIDVNGIVVFGTRILDEEVFEGDIQQLLLVSDHRAAYDYCEHYSPDCDTAVPDKPQSQDPNPDEYYPDGDNEGDTYYYEYPYYEDTDDTGKDPVPTKTPVEAARETTEVPEELTQPPTEAPSAPSTTEGPGKEEDPGIGDYDYVPSEDYYTPPPYEDFGYGEGIENPDENPDQHPDQGARAEVPTSTVLTSNGSNPAPPPEEGMDDLEGEFTEETIKNLDESYYDPYYDPTISPSEVGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLLEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGEDGERGDDGEAGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGAPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGERGVPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGQPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLMDDAEGEQYMDYADGMEEIFGSLNSLKLEIEQMKRPLGTPQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNITYNCYQSVAWQDAATGSYDKAMRFLGSNDEEMSYDNSPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_027966117.1
Organism	Ovis aries(sheep)
Definition	Ovis aries collagen type V alpha 1 chain (COL5A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966117.1

ORF Insert Sequence:

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGCTGTGGGC GCCGCCTCCG 
AGCCGCGCAG CTCAGCCAGC AGATCTCCTG AAGGTTCTAG ATTTTCACAA CCTGCCTGAT 
GGAATAACCA AGACCACAGG CTTCTGTGCA GCGCGGCGAT CTTCCAAAGG CCCGGATGTC 
GCCTACAGAG TCACGAAAGA TGCCCAGCTC AGCGCGCCCA CCAAGCAGCT GTACCCTGCA 
TCTGCGTTTC CTGAGGACTT CTCCATCCTA ACCACTGTGA AAGCCAAGAA AGGCAGCCAG 
GCCTTCCTGG TCTCCATCTA TAACGAGCAG GGCATCCAGC AGATCGGCCT GGAGATGGGA 
CGCTCCCCGG TCTTCCTCTA CGAGGACCAC ACGGGGAAGC CGGGGCCAGA GGACTACCCC 
CTCTTCAGAG GCATCAACCT GTCGGATGGC AAGTGGCACA GAATCGCTCT CAGCATCCAC 
AAGAAGAATG TCACCTTGAT CCTGGACTGT AAAAAGAAGA CGACCAAGTT CCTGGACCGC 
AGCGAACACC CTGTGATAGA CGTCAACGGC ATCGTTGTAT TCGGCACCCG GATTCTGGAT 
GAAGAAGTGT TTGAGGGTGA CATCCAGCAG CTGCTCCTCG TCTCGGACCA CCGGGCGGCT 
TACGATTACT GTGAGCATTA CAGCCCAGAC TGCGACACGG CCGTCCCCGA CAAACCCCAG 
TCTCAGGACC CCAACCCAGA CGAATACTAC CCAGATGGGG ACAATGAAGG AGACACCTAT 
TACTACGAGT ACCCCTACTA CGAGGACACG GACGACACGG GCAAGGACCC CGTCCCCACC 
AAGACGCCCG TGGAAGCTGC TCGGGAGACC ACGGAGGTGC CCGAGGAGCT GACCCAGCCC 
CCAACAGAAG CCCCTTCAGC GCCCAGCACC ACGGAGGGCC CTGGGAAGGA GGAGGACCCC 
GGCATCGGCG ACTATGACTA CGTGCCCAGT GAGGACTACT ACACGCCACC CCCTTACGAG 
GACTTCGGCT ACGGTGAAGG CATCGAGAAC CCGGATGAGA ACCCTGACCA GCACCCGGAC 
CAGGGCGCCC GGGCTGAGGT TCCCACCAGC ACGGTCCTCA CCTCCAACGG CTCCAACCCG 
GCCCCGCCTC CGGAGGAGGG GATGGACGAC CTGGAGGGAG AGTTCACGGA GGAAACCATC 
AAGAACCTGG ACGAGAGCTA CTACGACCCG TACTACGACC CCACCATCTC CCCATCGGAG 
GTCGGGCCAG GCATGCCCGC CAACCAGGAC ACCATCTATG AAGGGATCGG AGGCCCTCGG 
GGCGAGAAAG GCCAGAAAGG AGAGCCCGCC ATCATCGAGC CGGGCATGCT CCTGGAGGGG 
CCGCCCGGCC CCGAAGGCCC TGCGGGTCTC CCAGGACCTC CAGGGACGAT GGGTCCCACA 
GGCCAAGTGG GTGACCCTGG AGAAAGGGGT CCCCCTGGAC GCCCAGGCCT TCCTGGCGCT 
GACGGGCTGC CTGGCCCTCC TGGAACCATG CTCATGCTGC CTTTCCGGTT CGGAGGGGGC 
GGTGACGCGG GCTCCAAAGG ACCCATGGTC TCCGCGCAGG AGTCCCAAGC TCAAGCCATC 
CTGCAGCAGG CCCGGCTCGC GTTGAGGGGG CCTGCTGGCC CGATGGGTCT CACTGGGAGA 
CCGGGTCCCA TGGGCCCTCC TGGGAGCGGA GGTCTGAAGG GCGAGCCAGG AGACATGGGG 
CCTCAGGGTC CACGGGGTGT GCAGGGCCCG CCTGGCCCGG CAGGAAAGCC TGGGAGAAGG 
GGCCGAGCCG GCAGTGACGG AGCGAGAGGG ATGCCCGGGC AGACTGGCCC CAAGGGTGAC 
CGCGGCTTTG ATGGCCTGGC GGGGCTGCCA GGGGAGAAAG GCCACCGGGG TGACCCTGGC 
CCATCCGGCC CGCCGGGACC TCCAGGGGAA GACGGAGAAA GGGGCGACGA TGGAGAAGCT 
GGGCCCAGAG GGCTGCCTGG GGAGCCCGGG CCGCGAGGTC TGCTGGGGCC GAAGGGCCCC 
CCAGGCCCCC CTGGACCTCC CGGTGTGACA GGAATGGATG GTCAACCAGG CCCGAAAGGC 
AACGTGGGTC CCCAGGGAGA GCCCGGCCCC CCGGGGCAGC AGGGCAACCC AGGTGCCCAG 
GGTCTTCCGG GCCCCCAGGG CGCAATCGGG CCTCCAGGAG AAAAGGGTCC ACTGGGCAAA 
CCCGGCCTCC CAGGAATGCC TGGTGCTGAT GGACCCCCGG GGCACCCTGG CAAAGAAGGC 
CCTCCAGGAG AGAAGGGAGG GCAGGGTCCA CCTGGCCCCC AGGGTCCAAT TGGCTACCCA 
GGTCCTCGCG GAGTCAAGGG GGCGGACGGC ATCCGAGGTC TGAAGGGCAC CAAGGGCGAG 
AAGGGTGAAG ATGGCTTCCC GGGCTTCAAA GGAGACATGG GCATCAAGGG CGATCGGGGG 
GAGATCGGCC CACCTGGTCC CAGAGGGGAG GACGGTCCCG AAGGTCCCAA GGGACGAGGC 
GGTCCGAACG GCGACCCTGG TCCTCTGGGA CCCCCTGGGG AGAAGGGCAA ACTCGGAGTC 
CCAGGACTGC CAGGCTACCC AGGAAGACAG GGGCCAAAGG GTTCTATCGG ATTTCCTGGA 
TTTCCTGGCG CCAACGGAGA AAAGGGCGGC AGGGGCACCC CTGGGAAGCC GGGACCGCGG 
GGGCAGCGAG GCCCGACGGG CCCACGAGGT GAAAGAGGCC CACGGGGCAT AACCGGGAAG 
CCTGGCCCCA AGGGCAACTC TGGAGGCGAT GGCCCGGCTG GCCCTCCCGG TGAACGGGGA 
CCCAACGGAC CCCAAGGACC CACGGGGTTC CCTGGACCAA AGGGCCCCCC GGGGCCCCCA 
GGCAAGGACG GGCTCCCGGG ACACCCTGGA CAGCGAGGCG AGACGGGTTT CCAAGGCAAG 
ACCGGCCCTC CAGGCCCTCC GGGTGTGGTC GGCCCTCAGG GTCCCACTGG GGAAACTGGT 
CCCATGGGCG AACGTGGCCA CCCTGGTCCC CCAGGGCCCC CCGGTGAACA GGGGCTCCCG 
GGCCTGGCCG GGAAAGAAGG GACGAAGGGA GACCCAGGCC CCGCCGGCCT TCCTGGGAAA 
GACGGTCCCC CCGGGCTGCG AGGCTTCCCT GGAGACCGAG GACTTCCTGG TCCAGTGGGG 
GCGCTTGGAC TGAAAGGCAG TGAAGGCCCC CCCGGCCCGC CAGGCCCCGC GGGATCTCCA 
GGGGAGAGAG GTCCTGCCGG TGCCGCCGGG CCCATTGGAA TTCCAGGGAG GCCCGGGCCC 
CAGGGGCCCC CTGGGCCAGC TGGGGAGAAA GGCGCCCCTG GCGAGAAGGG TCCACAAGGC 
CCAGCTGGCC GAGATGGTCT CCAGGGCCCC GTGGGGCTCC CCGGTCCCGC TGGCCCTGTG 
GGCCCCCCTG GAGAAGACGG AGACAAGGGA GAGATTGGAG AGCCAGGACA GAAGGGCAGC 
AAGGGAGACA AAGGCGAGCA GGGTCCACCA GGGCCTACAG GACCTCAAGG CCCCATCGGA 
CAGCCAGGCC CCTCCGGAGC TGATGGCGAA CCGGGACCTC GGGGCCAGCA GGGCCTCTTT 
GGGCAGAAAG GCGACGAAGG TCCGAGAGGC TTCCCCGGGC CCCCGGGTCC TGTGGGGCTG 
CAGGGTCTGC CGGGACCTCC TGGAGAGAAG GGCGAGACGG GTGACGTGGG TCAGATGGGC 
CCGCCAGGTC CCCCCGGCCC CCGAGGACCC TCCGGAGCTC CAGGTGCAGA TGGGCCACAA 
GGACCCCCCG GTGGAATAGG CAACCCCGGA GCCGTGGGAG AGAAGGGTGA GCCTGGCGAA 
GCGGGAGAGC CTGGCCTTCC CGGTGAAGGG GGCCCCCCGG GACCCAAAGG CGAAAGGGGG 
GAGAAGGGCG AGTCAGGCCC CTCCGGTGCC GCCGGACCCC CGGGGCCCAA GGGCCCTCCC 
GGAGACGACG GTCCCAAAGG CAGCCCCGGC CCCGTCGGTT TTCCCGGAGA TCCTGGTCCC 
CCCGGAGAAC CTGGCCCCGC GGGTCAAGAC GGTCCTCCTG GCGACAAAGG AGACGACGGT 
GAACCCGGGC AAACGGGATC ACCAGGCCCT ACGGGTGAGC CAGGCCCATC TGGGCCTCCA 
GGAAAAAGGG GTCCGCCCGG CCCCGCAGGC CCCGAAGGCA GGCAGGGGGA GAAAGGAGCC 
AAGGGAGAAG CCGGCTTGGA AGGCCCTCCT GGGAAGACTG GCCCCATCGG CCCCCAGGGG 
GCCCCCGGGA AGCCGGGGCC TGACGGCCTT CGAGGGATCC CCGGCCCAGT GGGTGAACAA 
GGTCTCCCTG GAGCCCCGGG CCCCGATGGC CCCCCAGGAC CCATGGGCCC CCCAGGACTC 
CCCGGCCTCA AAGGAGACTC TGGACCCAAA GGAGAAAAGG GTCACCCAGG CTTGATCGGC 
CTCATCGGAC CTCCGGGCGA ACAGGGAGAA AAGGGTGAAC GTGGAGTCCC CGGACCCCAG 
GGCTCCTCGG GCCCTAAGGG AGAACAGGGT ATCACTGGTC CTTCCGGCCC GATTGGCCCC 
CCAGGTCCCC CCGGCCTGCC GGGCCCCCCT GGTCCAAAAG GTGCTAAGGG CTCCTCGGGT 
CCAACTGGCC CGAAGGGTGA GGCAGGCCAG CCAGGACCCC CTGGCCCACC AGGCCCCCCG 
GGCGAGGTCA TCCAGCCCCT GCCGATCCAG GCGTCCAGGA CCCGGCGGAA CATCGACGCC 
AGCCAGCTGA TGGACGACGC AGAGGGCGAG CAGTACATGG ACTATGCGGA CGGCATGGAG 
GAGATCTTCG GCTCGCTCAA CTCCCTGAAG CTGGAGATCG AGCAGATGAA GCGGCCGCTG 
GGCACGCCGC AGAACCCCGC GCGCACCTGC AAAGACCTCC AGCTCTGCCA CCCCGACTTC 
CCCGATGGCG AATACTGGGT GGATCCGAAC CAAGGCTGCT CCCGGGACTC CTTCAAGGTG 
TACTGCAACT TCACGGCCGG GGGCTCGACG TGCGTCTTCC CCGACAAGAA GTCCGAGGGG 
GCCAGAATCA CTTCTTGGCC CAAAGAAAAC CCGGGTTCCT GGTTCAGTGA GTTCAAGCGT 
GGCAAACTGC TCTCCTACGT GGACGCCGAG GGCAACCCCG TGGGCGTGGT CCAGATGACC 
TTCCTGCGGC TGCTCAGCGC CTCCGCCCAC CAGAACATCA CCTACAACTG CTACCAGTCG 
GTGGCCTGGC AGGACGCCGC CACGGGCAGC TACGACAAGG CCATGCGCTT CCTGGGCTCC 
AACGACGAGG AGATGTCGTA TGACAACAGC CCCTACATCC GTGCCCTGGT GGACGGCTGC 
GCCACGAAGA AAGGGTATCA GAAGACGGTT CTGGAGATCG ACACGCCCAA GGTGGAGCAG 
GTGCCCATCG TCGACATTAT GTTCAACGAC TTCGGGGAAG CGTCACAGAA ATTCGGATTT 
GAAGTGGGGC CGGCTTGCTT CCTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( XM_027966118.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027821919.1 Ovis aries collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA.

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CloneID	OSm76412
Clone ID Related Accession (Same CDS sequence)	XM_027966118.1
Accession Version	XM_027966118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	collagen alpha-1(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGCTGTGGGC GCCGCCTCCG 
AGCCGCGCAG CTCAGCCAGC AGATCTCCTG AAGGTTCTAG ATTTTCACAA CCTGCCTGAT 
GGAATAACCA AGACCACAGG CTTCTGTGCA GCGCGGCGAT CTTCCAAAGG CCCGGATGTC 
GCCTACAGAG TCACGAAAGA TGCCCAGCTC AGCGCGCCCA CCAAGCAGCT GTACCCTGCA 
TCTGCGTTTC CTGAGGACTT CTCCATCCTA ACCACTGTGA AAGCCAAGAA AGGCAGCCAG 
GCCTTCCTGG TCTCCATCTA TAACGAGCAG GGCATCCAGC AGATCGGCCT GGAGATGGGA 
CGCTCCCCGG TCTTCCTCTA CGAGGACCAC ACGGGGAAGC CGGGGCCAGA GGACTACCCC 
CTCTTCAGAG GCATCAACCT GTCGGATGGC AAGTGGCACA GAATCGCTCT CAGCATCCAC 
AAGAAGAATG TCACCTTGAT CCTGGACTGT AAAAAGAAGA CGACCAAGTT CCTGGACCGC 
AGCGAACACC CTGTGATAGA CGTCAACGGC ATCGTTGTAT TCGGCACCCG GATTCTGGAT 
GAAGAAGTGT TTGAGGGTGA CATCCAGCAG CTGCTCCTCG TCTCGGACCA CCGGGCGGCT 
TACGATTACT GTGAGCATTA CAGCCCAGAC TGCGACACGG CCGTCCCCGA CAAACCCCAG 
TCTCAGGACC CCAACCCAGA CGAATACTAC CCAGATGGGG ACAATGAAGG AGACACCTAT 
TACTACGAGT ACCCCTACTA CGAGGACACG GACGACACGG GCAAGGACCC CGTCCCCACC 
AAGACGCCCG TGGAAGCTGC TCGGGAGACC ACGGAGGTGC CCGAGGAGCT GACCCAGCCC 
CCAACAGAAG CCCCTTCAGC GCCCAGCACC ACGGAGGGCC CTGGGAAGGA GGAGGACCCC 
GGCATCGGCG ACTATGACTA CGTGCCCAGT GAGGACTACT ACACGCCACC CCCTTACGAG 
GACTTCGGCT ACGGTGAAGG CATCGAGAAC CCGGATGAGA ACCCTGACCA GCACCCGGAC 
CAGGGCGCCC GGGCTGAGGT TCCCACCAGC ACGGTCCTCA CCTCCAACGG CTCCAACCCG 
GCCCCGCCTC CGGAGGAGGG GATGGACGAC CTGGAGGGAG AGTTCACGGA GGAAACCATC 
AAGAACCTGG ACGAGAGCTA CTACGACCCG TACTACGACC CCACCATCTC CCCATCGGAG 
GTCGGGCCAG GCATGCCCGC CAACCAGGAC ACCATCTATG AAGGGATCGG AGGCCCTCGG 
GGCGAGAAAG GCCAGAAAGG AGAGCCCGCC ATCATCGAGC CGGGCATGCT CCTGGAGGGG 
CCGCCCGGCC CCGAAGGCCC TGCGGGTCTC CCAGGACCTC CAGGGACGAT GGGTCCCACA 
GGCCAAGTGG GTGACCCTGG AGAAAGGGGT CCCCCTGGAC GCCCAGGCCT TCCTGGCGCT 
GACGGGCTGC CTGGCCCTCC TGGAACCATG CTCATGCTGC CTTTCCGGTT CGGAGGGGGC 
GGTGACGCGG GCTCCAAAGG ACCCATGGTC TCCGCGCAGG AGTCCCAAGC TCAAGCCATC 
CTGCAGCAGG CCCGGCTCGC GTTGAGGGGG CCTGCTGGCC CGATGGGTCT CACTGGGAGA 
CCGGGTCCCA TGGGCCCTCC TGGGAGCGGA GGTCTGAAGG GCGAGCCAGG AGACATGGGG 
CCTCAGGGTC CACGGGGTGT GCAGGGCCCG CCTGGCCCGG CAGGAAAGCC TGGGAGAAGG 
GGCCGAGCCG GCAGTGACGG AGCGAGAGGG ATGCCCGGGC AGACTGGCCC CAAGGGTGAC 
CGCGGCTTTG ATGGCCTGGC GGGGCTGCCA GGGGAGAAAG GCCACCGGGG TGACCCTGGC 
CCATCCGGCC CGCCGGGACC TCCAGGGGAA GACGGAGAAA GGGGCGACGA TGGAGAAGCT 
GGGCCCAGAG GGCTGCCTGG GGAGCCCGGG CCGCGAGGTC TGCTGGGGCC GAAGGGCCCC 
CCAGGCCCCC CTGGACCTCC CGGTGTGACA GGAATGGATG GTCAACCAGG CCCGAAAGGC 
AACGTGGGTC CCCAGGGAGA GCCCGGCCCC CCGGGGCAGC AGGGCAACCC AGGTGCCCAG 
GGTCTTCCGG GCCCCCAGGG CGCAATCGGG CCTCCAGGAG AAAAGGGTCC ACTGGGCAAA 
CCCGGCCTCC CAGGAATGCC TGGTGCTGAT GGACCCCCGG GGCACCCTGG CAAAGAAGGC 
CCTCCAGGAG AGAAGGGAGG GCAGGGTCCA CCTGGCCCCC AGGGTCCAAT TGGCTACCCA 
GGTCCTCGCG GAGTCAAGGG GGCGGACGGC ATCCGAGGTC TGAAGGGCAC CAAGGGCGAG 
AAGGGTGAAG ATGGCTTCCC GGGCTTCAAA GGAGACATGG GCATCAAGGG CGATCGGGGG 
GAGATCGGCC CACCTGGTCC CAGAGGGGAG GACGGTCCCG AAGGTCCCAA GGGACGAGGC 
GGTCCGAACG GCGACCCTGG TCCTCTGGGA CCCCCTGGGG AGAAGGGCAA ACTCGGAGTC 
CCAGGACTGC CAGGCTACCC AGGAAGACAG GGGCCAAAGG GTTCTATCGG ATTTCCTGGA 
TTTCCTGGCG CCAACGGAGA AAAGGGCGGC AGGGGCACCC CTGGGAAGCC GGGACCGCGG 
GGGCAGCGAG GCCCGACGGG CCCACGAGGT GAAAGAGGCC CACGGGGCAT AACCGGGAAG 
CCTGGCCCCA AGGGCAACTC TGGAGGCGAT GGCCCGGCTG GCCCTCCCGG TGAACGGGGA 
CCCAACGGAC CCCAAGGACC CACGGGGTTC CCTGGACCAA AGGGCCCCCC GGGGCCCCCA 
GGCAAGGACG GGCTCCCGGG ACACCCTGGA CAGCGAGGCG AGACGGGTTT CCAAGGCAAG 
ACCGGCCCTC CAGGCCCTCC GGGTGTGGTC GGCCCTCAGG GTCCCACTGG GGAAACTGGT 
CCCATGGGCG AACGTGGCCA CCCTGGTCCC CCAGGGCCCC CCGGTGAACA GGGGCTCCCG 
GGCCTGGCCG GGAAAGAAGG GACGAAGGGA GACCCAGGCC CCGCCGGCCT TCCTGGGAAA 
GACGGTCCCC CCGGGCTGCG AGGCTTCCCT GGAGACCGAG GACTTCCTGG TCCAGTGGGG 
GCGCTTGGAC TGAAAGGCAG TGAAGGCCCC CCCGGCCCGC CAGGCCCCGC GGGATCTCCA 
GGGGAGAGAG GTCCTGCCGG TGCCGCCGGG CCCATTGGAA TTCCAGGGAG GCCCGGGCCC 
CAGGGGCCCC CTGGGCCAGC TGGGGAGAAA GGCGCCCCTG GCGAGAAGGG TCCACAAGGC 
CCAGCTGGCC GAGATGGTCT CCAGGGCCCC GTGGGGCTCC CCGGTCCCGC TGGCCCTGTG 
GGCCCCCCTG GAGAAGACGG AGACAAGGGA GAGATTGGAG AGCCAGGACA GAAGGGCAGC 
AAGGGAGACA AAGGCGAGCA GGGTCCACCA GGGCCTACAG GACCTCAAGG CCCCATCGGA 
CAGCCAGGCC CCTCCGGAGC TGATGGCGAA CCGGGACCTC GGGGCCAGCA GGGCCTCTTT 
GGGCAGAAAG GCGACGAAGG TCCGAGAGGC TTCCCCGGGC CCCCGGGTCC TGTGGGGCTG 
CAGGGTCTGC CGGGACCTCC TGGAGAGAAG GGCGAGACGG GTGACGTGGG TCAGATGGGC 
CCGCCAGGTC CCCCCGGCCC CCGAGGACCC TCCGGAGCTC CAGGTGCAGA TGGGCCACAA 
GGACCCCCCG GTGGAATAGG CAACCCCGGA GCCGTGGGAG AGAAGGGTGA GCCTGGCGAA 
GCGGGAGAGC CTGGCCTTCC CGGTGAAGGG GGCCCCCCGG GACCCAAAGG CGAAAGGGGG 
GAGAAGGGCG AGTCAGGCCC CTCCGGTGCC GCCGGACCCC CGGGGCCCAA GGGCCCTCCC 
GGAGACGACG GTCCCAAAGG CAGCCCCGGC CCCGTCGGTT TTCCCGGAGA TCCTGGTCCC 
CCCGGAGAAC CTGGCCCCGC GGGTCAAGAC GGTCCTCCTG GCGACAAAGG AGACGACGGT 
GAACCCGGGC AAACGGGATC ACCAGGCCCT ACGGGTGAGC CAGGCCCATC TGGGCCTCCA 
GGAAAAAGGG GTCCGCCCGG CCCCGCAGGC CCCGAAGGCA GGCAGGGGGA GAAAGGAGCC 
AAGGGAGAAG CCGGCTTGGA AGGCCCTCCT GGGAAGACTG GCCCCATCGG CCCCCAGGGG 
GCCCCCGGGA AGCCGGGGCC TGACGGCCTT CGAGGGATCC CCGGCCCAGT GGGTGAACAA 
GGTCTCCCTG GAGCCCCGGG CCCCGATGGC CCCCCAGGAC CCATGGGCCC CCCAGGACTC 
CCCGGCCTCA AAGGAGACTC TGGACCCAAA GGAGAAAAGG GTCACCCAGG CTTGATCGGC 
CTCATCGGAC CTCCGGGCGA ACAGGGAGAA AAGGGTGAAC GTGGAGTCCC CGGACCCCAG 
GGCTCCTCGG GCCCTAAGGG AGAACAGGGT ATCACTGGTC CTTCCGGCCC GATTGGCCCC 
CCAGGTCCCC CCGGCCTGCC GGGCCCCCCT GGTCCAAAAG GTGCTAAGGG CTCCTCGGGT 
CCAACTGGCC CGAAGGGTGA GGCAGGCCAG CCAGGACCCC CTGGCCCACC AGGCCCCCCG 
GGCGAGGTCA TCCAGCCCCT GCCGATCCAG GCGTCCAGGA CCCGGCGGAA CATCGACGCC 
AGCCAGCTGA TGGACGACGC AGAGGGCGAG CAGTACATGG ACTATGCGGA CGGCATGGAG 
GAGATCTTCG GCTCGCTCAA CTCCCTGAAG CTGGAGATCG AGCAGATGAA GCGGCCGCTG 
GGCACGCCGC AGAACCCCGC GCGCACCTGC AAAGACCTCC AGCTCTGCCA CCCCGACTTC 
CCCGATGGCG AATACTGGGT GGATCCGAAC CAAGGCTGCT CCCGGGACTC CTTCAAGGTG 
TACTGCAACT TCACGGCCGG GGGCTCGACG TGCGTCTTCC CCGACAAGAA GTCCGAGGGG 
AGTAAGATGG CCCGCTGGCC CAAAGAGCAG CCCTCCACCT GGTACAGTCA GTACAAGCGG 
GGGTCGCTGC TCTCCTACGT GGACGCCGAG GGCAACCCCG TGGGCGTGGT CCAGATGACC 
TTCCTGCGGC TGCTCAGCGC CTCCGCCCAC CAGAACATCA CCTACAACTG CTACCAGTCG 
GTGGCCTGGC AGGACGCCGC CACGGGCAGC TACGACAAGG CCATGCGCTT CCTGGGCTCC 
AACGACGAGG AGATGTCGTA TGACAACAGC CCCTACATCC GTGCCCTGGT GGACGGCTGC 
GCCACGAAGA AAGGGTATCA GAAGACGGTT CTGGAGATCG ACACGCCCAA GGTGGAGCAG 
GTGCCCATCG TCGACATTAT GTTCAACGAC TTCGGGGAAG CGTCACAGAA ATTCGGATTT 
GAAGTGGGGC CGGCTTGCTT CCTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027821919.1
CDS	410..5959
Translation	MDFHTRWKARSPLRPGAPLLSPLPPPLLLLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCAARRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLEMGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSIHKKNVTLILDCKKKTTKFLDRSEHPVIDVNGIVVFGTRILDEEVFEGDIQQLLLVSDHRAAYDYCEHYSPDCDTAVPDKPQSQDPNPDEYYPDGDNEGDTYYYEYPYYEDTDDTGKDPVPTKTPVEAARETTEVPEELTQPPTEAPSAPSTTEGPGKEEDPGIGDYDYVPSEDYYTPPPYEDFGYGEGIENPDENPDQHPDQGARAEVPTSTVLTSNGSNPAPPPEEGMDDLEGEFTEETIKNLDESYYDPYYDPTISPSEVGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLLEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGEDGERGDDGEAGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGAPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGERGVPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGQPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLMDDAEGEQYMDYADGMEEIFGSLNSLKLEIEQMKRPLGTPQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGVVQMTFLRLLSASAHQNITYNCYQSVAWQDAATGSYDKAMRFLGSNDEEMSYDNSPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_027966118.1
Organism	Ovis aries(sheep)
Definition	Ovis aries collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966118.1

ORF Insert Sequence:

ATGGACTTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
TCCCCGCTGC CGCCGCCGCT GCTGCTGCTG CTGCTGCTGC TGCTGTGGGC GCCGCCTCCG 
AGCCGCGCAG CTCAGCCAGC AGATCTCCTG AAGGTTCTAG ATTTTCACAA CCTGCCTGAT 
GGAATAACCA AGACCACAGG CTTCTGTGCA GCGCGGCGAT CTTCCAAAGG CCCGGATGTC 
GCCTACAGAG TCACGAAAGA TGCCCAGCTC AGCGCGCCCA CCAAGCAGCT GTACCCTGCA 
TCTGCGTTTC CTGAGGACTT CTCCATCCTA ACCACTGTGA AAGCCAAGAA AGGCAGCCAG 
GCCTTCCTGG TCTCCATCTA TAACGAGCAG GGCATCCAGC AGATCGGCCT GGAGATGGGA 
CGCTCCCCGG TCTTCCTCTA CGAGGACCAC ACGGGGAAGC CGGGGCCAGA GGACTACCCC 
CTCTTCAGAG GCATCAACCT GTCGGATGGC AAGTGGCACA GAATCGCTCT CAGCATCCAC 
AAGAAGAATG TCACCTTGAT CCTGGACTGT AAAAAGAAGA CGACCAAGTT CCTGGACCGC 
AGCGAACACC CTGTGATAGA CGTCAACGGC ATCGTTGTAT TCGGCACCCG GATTCTGGAT 
GAAGAAGTGT TTGAGGGTGA CATCCAGCAG CTGCTCCTCG TCTCGGACCA CCGGGCGGCT 
TACGATTACT GTGAGCATTA CAGCCCAGAC TGCGACACGG CCGTCCCCGA CAAACCCCAG 
TCTCAGGACC CCAACCCAGA CGAATACTAC CCAGATGGGG ACAATGAAGG AGACACCTAT 
TACTACGAGT ACCCCTACTA CGAGGACACG GACGACACGG GCAAGGACCC CGTCCCCACC 
AAGACGCCCG TGGAAGCTGC TCGGGAGACC ACGGAGGTGC CCGAGGAGCT GACCCAGCCC 
CCAACAGAAG CCCCTTCAGC GCCCAGCACC ACGGAGGGCC CTGGGAAGGA GGAGGACCCC 
GGCATCGGCG ACTATGACTA CGTGCCCAGT GAGGACTACT ACACGCCACC CCCTTACGAG 
GACTTCGGCT ACGGTGAAGG CATCGAGAAC CCGGATGAGA ACCCTGACCA GCACCCGGAC 
CAGGGCGCCC GGGCTGAGGT TCCCACCAGC ACGGTCCTCA CCTCCAACGG CTCCAACCCG 
GCCCCGCCTC CGGAGGAGGG GATGGACGAC CTGGAGGGAG AGTTCACGGA GGAAACCATC 
AAGAACCTGG ACGAGAGCTA CTACGACCCG TACTACGACC CCACCATCTC CCCATCGGAG 
GTCGGGCCAG GCATGCCCGC CAACCAGGAC ACCATCTATG AAGGGATCGG AGGCCCTCGG 
GGCGAGAAAG GCCAGAAAGG AGAGCCCGCC ATCATCGAGC CGGGCATGCT CCTGGAGGGG 
CCGCCCGGCC CCGAAGGCCC TGCGGGTCTC CCAGGACCTC CAGGGACGAT GGGTCCCACA 
GGCCAAGTGG GTGACCCTGG AGAAAGGGGT CCCCCTGGAC GCCCAGGCCT TCCTGGCGCT 
GACGGGCTGC CTGGCCCTCC TGGAACCATG CTCATGCTGC CTTTCCGGTT CGGAGGGGGC 
GGTGACGCGG GCTCCAAAGG ACCCATGGTC TCCGCGCAGG AGTCCCAAGC TCAAGCCATC 
CTGCAGCAGG CCCGGCTCGC GTTGAGGGGG CCTGCTGGCC CGATGGGTCT CACTGGGAGA 
CCGGGTCCCA TGGGCCCTCC TGGGAGCGGA GGTCTGAAGG GCGAGCCAGG AGACATGGGG 
CCTCAGGGTC CACGGGGTGT GCAGGGCCCG CCTGGCCCGG CAGGAAAGCC TGGGAGAAGG 
GGCCGAGCCG GCAGTGACGG AGCGAGAGGG ATGCCCGGGC AGACTGGCCC CAAGGGTGAC 
CGCGGCTTTG ATGGCCTGGC GGGGCTGCCA GGGGAGAAAG GCCACCGGGG TGACCCTGGC 
CCATCCGGCC CGCCGGGACC TCCAGGGGAA GACGGAGAAA GGGGCGACGA TGGAGAAGCT 
GGGCCCAGAG GGCTGCCTGG GGAGCCCGGG CCGCGAGGTC TGCTGGGGCC GAAGGGCCCC 
CCAGGCCCCC CTGGACCTCC CGGTGTGACA GGAATGGATG GTCAACCAGG CCCGAAAGGC 
AACGTGGGTC CCCAGGGAGA GCCCGGCCCC CCGGGGCAGC AGGGCAACCC AGGTGCCCAG 
GGTCTTCCGG GCCCCCAGGG CGCAATCGGG CCTCCAGGAG AAAAGGGTCC ACTGGGCAAA 
CCCGGCCTCC CAGGAATGCC TGGTGCTGAT GGACCCCCGG GGCACCCTGG CAAAGAAGGC 
CCTCCAGGAG AGAAGGGAGG GCAGGGTCCA CCTGGCCCCC AGGGTCCAAT TGGCTACCCA 
GGTCCTCGCG GAGTCAAGGG GGCGGACGGC ATCCGAGGTC TGAAGGGCAC CAAGGGCGAG 
AAGGGTGAAG ATGGCTTCCC GGGCTTCAAA GGAGACATGG GCATCAAGGG CGATCGGGGG 
GAGATCGGCC CACCTGGTCC CAGAGGGGAG GACGGTCCCG AAGGTCCCAA GGGACGAGGC 
GGTCCGAACG GCGACCCTGG TCCTCTGGGA CCCCCTGGGG AGAAGGGCAA ACTCGGAGTC 
CCAGGACTGC CAGGCTACCC AGGAAGACAG GGGCCAAAGG GTTCTATCGG ATTTCCTGGA 
TTTCCTGGCG CCAACGGAGA AAAGGGCGGC AGGGGCACCC CTGGGAAGCC GGGACCGCGG 
GGGCAGCGAG GCCCGACGGG CCCACGAGGT GAAAGAGGCC CACGGGGCAT AACCGGGAAG 
CCTGGCCCCA AGGGCAACTC TGGAGGCGAT GGCCCGGCTG GCCCTCCCGG TGAACGGGGA 
CCCAACGGAC CCCAAGGACC CACGGGGTTC CCTGGACCAA AGGGCCCCCC GGGGCCCCCA 
GGCAAGGACG GGCTCCCGGG ACACCCTGGA CAGCGAGGCG AGACGGGTTT CCAAGGCAAG 
ACCGGCCCTC CAGGCCCTCC GGGTGTGGTC GGCCCTCAGG GTCCCACTGG GGAAACTGGT 
CCCATGGGCG AACGTGGCCA CCCTGGTCCC CCAGGGCCCC CCGGTGAACA GGGGCTCCCG 
GGCCTGGCCG GGAAAGAAGG GACGAAGGGA GACCCAGGCC CCGCCGGCCT TCCTGGGAAA 
GACGGTCCCC CCGGGCTGCG AGGCTTCCCT GGAGACCGAG GACTTCCTGG TCCAGTGGGG 
GCGCTTGGAC TGAAAGGCAG TGAAGGCCCC CCCGGCCCGC CAGGCCCCGC GGGATCTCCA 
GGGGAGAGAG GTCCTGCCGG TGCCGCCGGG CCCATTGGAA TTCCAGGGAG GCCCGGGCCC 
CAGGGGCCCC CTGGGCCAGC TGGGGAGAAA GGCGCCCCTG GCGAGAAGGG TCCACAAGGC 
CCAGCTGGCC GAGATGGTCT CCAGGGCCCC GTGGGGCTCC CCGGTCCCGC TGGCCCTGTG 
GGCCCCCCTG GAGAAGACGG AGACAAGGGA GAGATTGGAG AGCCAGGACA GAAGGGCAGC 
AAGGGAGACA AAGGCGAGCA GGGTCCACCA GGGCCTACAG GACCTCAAGG CCCCATCGGA 
CAGCCAGGCC CCTCCGGAGC TGATGGCGAA CCGGGACCTC GGGGCCAGCA GGGCCTCTTT 
GGGCAGAAAG GCGACGAAGG TCCGAGAGGC TTCCCCGGGC CCCCGGGTCC TGTGGGGCTG 
CAGGGTCTGC CGGGACCTCC TGGAGAGAAG GGCGAGACGG GTGACGTGGG TCAGATGGGC 
CCGCCAGGTC CCCCCGGCCC CCGAGGACCC TCCGGAGCTC CAGGTGCAGA TGGGCCACAA 
GGACCCCCCG GTGGAATAGG CAACCCCGGA GCCGTGGGAG AGAAGGGTGA GCCTGGCGAA 
GCGGGAGAGC CTGGCCTTCC CGGTGAAGGG GGCCCCCCGG GACCCAAAGG CGAAAGGGGG 
GAGAAGGGCG AGTCAGGCCC CTCCGGTGCC GCCGGACCCC CGGGGCCCAA GGGCCCTCCC 
GGAGACGACG GTCCCAAAGG CAGCCCCGGC CCCGTCGGTT TTCCCGGAGA TCCTGGTCCC 
CCCGGAGAAC CTGGCCCCGC GGGTCAAGAC GGTCCTCCTG GCGACAAAGG AGACGACGGT 
GAACCCGGGC AAACGGGATC ACCAGGCCCT ACGGGTGAGC CAGGCCCATC TGGGCCTCCA 
GGAAAAAGGG GTCCGCCCGG CCCCGCAGGC CCCGAAGGCA GGCAGGGGGA GAAAGGAGCC 
AAGGGAGAAG CCGGCTTGGA AGGCCCTCCT GGGAAGACTG GCCCCATCGG CCCCCAGGGG 
GCCCCCGGGA AGCCGGGGCC TGACGGCCTT CGAGGGATCC CCGGCCCAGT GGGTGAACAA 
GGTCTCCCTG GAGCCCCGGG CCCCGATGGC CCCCCAGGAC CCATGGGCCC CCCAGGACTC 
CCCGGCCTCA AAGGAGACTC TGGACCCAAA GGAGAAAAGG GTCACCCAGG CTTGATCGGC 
CTCATCGGAC CTCCGGGCGA ACAGGGAGAA AAGGGTGAAC GTGGAGTCCC CGGACCCCAG 
GGCTCCTCGG GCCCTAAGGG AGAACAGGGT ATCACTGGTC CTTCCGGCCC GATTGGCCCC 
CCAGGTCCCC CCGGCCTGCC GGGCCCCCCT GGTCCAAAAG GTGCTAAGGG CTCCTCGGGT 
CCAACTGGCC CGAAGGGTGA GGCAGGCCAG CCAGGACCCC CTGGCCCACC AGGCCCCCCG 
GGCGAGGTCA TCCAGCCCCT GCCGATCCAG GCGTCCAGGA CCCGGCGGAA CATCGACGCC 
AGCCAGCTGA TGGACGACGC AGAGGGCGAG CAGTACATGG ACTATGCGGA CGGCATGGAG 
GAGATCTTCG GCTCGCTCAA CTCCCTGAAG CTGGAGATCG AGCAGATGAA GCGGCCGCTG 
GGCACGCCGC AGAACCCCGC GCGCACCTGC AAAGACCTCC AGCTCTGCCA CCCCGACTTC 
CCCGATGGCG AATACTGGGT GGATCCGAAC CAAGGCTGCT CCCGGGACTC CTTCAAGGTG 
TACTGCAACT TCACGGCCGG GGGCTCGACG TGCGTCTTCC CCGACAAGAA GTCCGAGGGG 
AGTAAGATGG CCCGCTGGCC CAAAGAGCAG CCCTCCACCT GGTACAGTCA GTACAAGCGG 
GGGTCGCTGC TCTCCTACGT GGACGCCGAG GGCAACCCCG TGGGCGTGGT CCAGATGACC 
TTCCTGCGGC TGCTCAGCGC CTCCGCCCAC CAGAACATCA CCTACAACTG CTACCAGTCG 
GTGGCCTGGC AGGACGCCGC CACGGGCAGC TACGACAAGG CCATGCGCTT CCTGGGCTCC 
AACGACGAGG AGATGTCGTA TGACAACAGC CCCTACATCC GTGCCCTGGT GGACGGCTGC 
GCCACGAAGA AAGGGTATCA GAAGACGGTT CTGGAGATCG ACACGCCCAA GGTGGAGCAG 
GTGCCCATCG TCGACATTAT GTTCAACGAC TTCGGGGAAG CGTCACAGAA ATTCGGATTT 
GAAGTGGGGC CGGCTTGCTT CCTGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL5A1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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