Pkia cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkia
Entrez Gene ID	18767
Full Name	protein kinase inhibitor, alpha
Synonyms	AI415001,PKIalpha
General protein information	Preferred Names protein kinase inhibitor, alpha Names cAMP-dependent protein kinase inhibitor alpha PKI-alpha cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530056.1	XP_006530119.1	cAMP-dependent protein kinase inhibitor alpha isoform X1
NM_008862.3	NP_032888.1	cAMP-dependent protein kinase inhibitor alpha
XM_030252461.1	XP_030108321.1	cAMP-dependent protein kinase inhibitor alpha isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PKIA	NP_862822.1
Canis lupus familiaris (dog)	PKIA	XP_853383.2
Xenopus tropicalis (tropical clawed frog)	pkia	NP_001025580.1
Bos taurus (cattle)	PKIA	NP_001032709.1
Rattus norvegicus (Norway rat)	Pkia	NP_446224.1
Pan troglodytes (chimpanzee)	PKIA	XP_003311822.1
Macaca mulatta (Rhesus monkey)	PKIA	XP_002805436.1
Gallus gallus (chicken)	PKIA	NP_990705.1
Mus musculus (house mouse)	Pkia	NP_032888.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Uncoupling Catalytic and Binding Functions in the Cyclic AMP-Dependent Protein Kinase A.
Kim J, Li G, Walters MA, Taylor SS, Veglia G
Structure (London, England : 1993)24(3)353-63(2016 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Cardiotoxic and cardioprotective features of chronic β-adrenergic signaling.
Zhang X, Szeto C, Gao E, Tang M, Jin J, Fu Q, Makarewich C, Ai X, Li Y, Tang A, Wang J, Gao H, Wang F, Ge XJ, Kunapuli SP, Zhou L, Zeng C, Xiang KY, Chen X
Circulation research112(3)498-509(2013 Feb)

Gene discovery by microarray: identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation.
Kuninger D, Kuzmickas R, Peng B, Pintar JE, Rotwein P
Genomics84(5)876-89(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pkia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31267	XM_006530056.1 Latest version!	Mus musculus protein kinase inhibitor, alpha (Pkia), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu02940	NM_008862.3 Latest version!	Mus musculus protein kinase inhibitor, alpha (Pkia), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OMu02940	XM_030252461.1 Latest version!	Mus musculus protein kinase inhibitor, alpha (Pkia), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkia ( XM_006530056.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530119.1 Mus musculus protein kinase inhibitor, alpha (Pkia), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31267
Clone ID Related Accession (Same CDS sequence)	XM_006530056.1
Accession Version	XM_006530056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	cAMP-dependent protein kinase inhibitor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAAA GTCACTATAA AAACCATCTT CGCAGCTTCA TAGGCTCAGC TTCTTCTAGG 
ATACACCGAT GTGGAAAGCT TCGATTTAAG ATAGGAGGCT CCCTGCTATG TGGATATTTG 
TTAGCAATGA CTGATGTGGA AACTACGTAT GCAGATTTCA TTGCTTCAGG AAGAACAGGT 
AGAAGAAATG CAATACATGA TATCCTGGTT TCCTCTGCAA GTGGCAACAG CAATGAATTA 
GCCTTAAAAC TAGCAGGCCT TGATATCAAC AAGACAGAAG GTGAAGATGA TGGACAGAGA 
AGCTCCACCG AACAAAGTGG AGAAGCCCAG GGAGAAGCAG CCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530119.1
CDS	5..361
Translation	MTQSHYKNHLRSFIGSASSRIHRCGKLRFKIGGSLLCGYLLAMTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	XM_006530056.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase inhibitor, alpha (Pkia), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530056.1

ORF Insert Sequence:

ATGACTCAAA GTCACTATAA AAACCATCTT CGCAGCTTCA TAGGCTCAGC TTCTTCTAGG 
ATACACCGAT GTGGAAAGCT TCGATTTAAG ATAGGAGGCT CCCTGCTATG TGGATATTTG 
TTAGCAATGA CTGATGTGGA AACTACGTAT GCAGATTTCA TTGCTTCAGG AAGAACAGGT 
AGAAGAAATG CAATACATGA TATCCTGGTT TCCTCTGCAA GTGGCAACAG CAATGAATTA 
GCCTTAAAAC TAGCAGGCCT TGATATCAAC AAGACAGAAG GTGAAGATGA TGGACAGAGA 
AGCTCCACCG AACAAAGTGG AGAAGCCCAG GGAGAAGCAG CCAAGTCTGA AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkia ( NM_008862.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032888.1 Mus musculus protein kinase inhibitor, alpha (Pkia), mRNA.

Price & Availability↑

CloneID	OMu02940
Clone ID Related Accession (Same CDS sequence)	NM_008862.3 , XM_030252461.1
Accession Version	NM_008862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	cAMP-dependent protein kinase inhibitor alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY253073.1, BC048244.1 and M63554.1. On Sep 1, 2006 this sequence version replaced NM_008862.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048244.1, SRR1660815.250573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACTGATG TGGAAACTAC GTATGCAGAT TTCATTGCTT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCCT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTA 
AAACTAGCAG GCCTTGATAT CAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACCGAACAAA GTGGAGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032888.1
CDS	177..407
Translation	MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	NM_008862.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase inhibitor, alpha (Pkia), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008862.3

ORF Insert Sequence:

ATGACTGATG TGGAAACTAC GTATGCAGAT TTCATTGCTT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCCT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTA 
AAACTAGCAG GCCTTGATAT CAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACCGAACAAA GTGGAGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkia ( XM_030252461.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108321.1 Mus musculus protein kinase inhibitor, alpha (Pkia), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu02940
Clone ID Related Accession (Same CDS sequence)	NM_008862.3 , XM_030252461.1
Accession Version	XM_030252461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	cAMP-dependent protein kinase inhibitor alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGATG TGGAAACTAC GTATGCAGAT TTCATTGCTT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCCT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTA 
AAACTAGCAG GCCTTGATAT CAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACCGAACAAA GTGGAGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108321.1
CDS	179..409
Translation	MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEDDGQRSSTEQSGEAQGEAAKSES

Target ORF information:

RefSeq Version	XM_030252461.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase inhibitor, alpha (Pkia), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252461.1

ORF Insert Sequence:

ATGACTGATG TGGAAACTAC GTATGCAGAT TTCATTGCTT CAGGAAGAAC AGGTAGAAGA 
AATGCAATAC ATGATATCCT GGTTTCCTCT GCAAGTGGCA ACAGCAATGA ATTAGCCTTA 
AAACTAGCAG GCCTTGATAT CAACAAGACA GAAGGTGAAG ATGATGGACA GAGAAGCTCC 
ACCGAACAAA GTGGAGAAGC CCAGGGAGAA GCAGCCAAGT CTGAAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Uncoupling Catalytic and Binding Functions in the Cyclic AMP-Dependent Protein Kinase A.
Structure (London, England : 1993)24(3)353-63(2016 Mar)
Kim J,Li G,Walters MA,Taylor SS,Veglia G

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Cardiotoxic and cardioprotective features of chronic β-adrenergic signaling.
Circulation research112(3)498-509(2013 Feb)
Zhang X,Szeto C,Gao E,Tang M,Jin J,Fu Q,Makarewich C,Ai X,Li Y,Tang A,Wang J,Gao H,Wang F,Ge XJ,Kunapuli SP,Zhou L,Zeng C,Xiang KY,Chen X

Gene discovery by microarray: identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation.
Genomics84(5)876-89(2004 Nov)
Kuninger D,Kuzmickas R,Peng B,Pintar JE,Rotwein P

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Pkia cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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