Myh11 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh11
Entrez Gene ID	17880
Full Name	myosin, heavy polypeptide 11, smooth muscle
Synonyms	AV071570,SM1,SM2,smMHC
General protein information	Preferred Names myosin, heavy polypeptide 11, smooth muscle Names myosin-11 myosin heavy chain 11, smooth muscle myosin heavy chain, smooth muscle isoform
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A1\|16 9.71 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013607.2	NP_038635.2	myosin-11 isoform 1
XM_006521843.1	XP_006521906.1	myosin-11 isoform X1
XM_006521843.2	XP_006521906.1	myosin-11 isoform X1
XM_006521844.2	XP_006521907.1	myosin-11 isoform X2
XM_006521844.1	XP_006521907.1	myosin-11 isoform X2
XM_006521845.1	XP_006521908.1	myosin-11 isoform X3
NM_001161775.1	NP_001155247.1	myosin-11 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH11	NP_001035203.1
Danio rerio (zebrafish)	myh11a	NP_001019619.1
Xenopus tropicalis (tropical clawed frog)	LOC100490639	XP_002932251.1
Rattus norvegicus (Norway rat)	Myh11	NP_001164071.1
Macaca mulatta (Rhesus monkey)	MYH11	XP_001109463.2
Pan troglodytes (chimpanzee)	LOC453949	XP_003952852.1
Canis lupus familiaris (dog)	MYH11	XP_005621571.1
Bos taurus (cattle)	MYH11	NP_001095597.1
Mus musculus (house mouse)	Myh11	NP_001155247.1
Gallus gallus (chicken)	MYH11	NP_990605.1

Gene Ontology
Bibliography

Related articles in PubMed

Delayed onset of smooth muscle cell differentiation leads to hydroureter formation in mice with conditional loss of the zinc finger transcription factor gene Gata2 in the ureteric mesenchyme.
Weiss AC, Bohnenpoll T, Kurz J, Blank P, Airik R, Lüdtke TH, Kleppa MJ, Deuper L, Kaiser M, Mamo TM, Costa R, von Hahn T, Trowe MO, Kispert A
The Journal of pathology248(4)452-463(2019 Aug)

HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis.
Xia M, Luo W, Jin H, Yang Z
Development (Cambridge, England)146(13)(2019 Jul)

Tie2 regulates endocardial sprouting and myocardial trabeculation.
Qu X, Harmelink C, Baldwin HS
JCI insight5(2019 May)

FOXF1 transcription factor promotes lung morphogenesis by inducing cellular proliferation in fetal lung mesenchyme.
Ustiyan V, Bolte C, Zhang Y, Han L, Xu Y, Yutzey KE, Zorn AM, Kalin TV, Shannon JM, Kalinichenko VV
Developmental biology443(1)50-63(2018 11)

TBX2 and TBX3 act downstream of canonical WNT signaling in patterning and differentiation of the mouse ureteric mesenchyme.
Aydoğdu N, Rudat C, Trowe MO, Kaiser M, Lüdtke TH, Taketo MM, Christoffels VM, Moon A, Kispert A
Development (Cambridge, England)145(23)(2018 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Myh11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu51916	XM_006521845.1 Latest version!	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu17533	NM_013607.2 Latest version!	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu17607	NM_001161775.1 Latest version!	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu51914	XM_006521843.2 Latest version!	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu51915	XM_006521844.2 Latest version!	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMu51914	XM_006521843.1	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu51915	XM_006521844.1	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh11 ( XM_006521845.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521908.1 Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu51916
Clone ID Related Accession (Same CDS sequence)	XM_006521845.1
Accession Version	XM_006521845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	myosin-11 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGAA AGCAAGCCTG CATTCTCATG ATCAAAGCCC TCGAACTTGA CCCTAACCTG 
TACAGGATTG GGCAGAGCAA AATCTTCTTC CGAACGGGGG TCCTGGCCCA CCTAGAGGAG 
GAACGAGACC TGAAAATTAC TGATGTCATC ATGGCCTTCC AGGCAATGTG TCGTGGCTAC 
CTTGCCAGAA AGGCCTTCAC CAAGAGGCAG CAACAGCTGA CAGCCATGAA GGTGATCCAG 
AGGAACTGCG CTGCCTACCT TAAGCTCCGC AACTGGCAAT GGTGGCGGCT CTTCACCAAA 
GTAAAGCCAT TGCTCCAGGT GACACGGCAG GAGGAGGAGA TGCAGGCCAA GGAGGAGGAG 
ATGCAAAAGA TCAAGGAGCG GCAGCAGAAG GCAGAGACTG AGTTGAAGGA GCTGGAGCAG 
AAGCACACTC AGCTGGCTGA GGAGAAGACT CTGCTGCAGG AGCAGTTGCA GGCAGAGACA 
GAGCTGTATG CTGAGGCTGA GGAGATGCGG GTCCGGTTGG CAGCCAAGAA GCAGGAACTG 
GAGGAGATCC TACATGAGAT GGAGGCCCGC CTGGAGGAAG AGGAAGACCG GGGCCAGCAA 
CTACAGGCTG AGAGGAAGAA GATGGCTCAG CAGATGCTAG ACCTGGAGGA GCAACTGGAG 
GAGGAAGAGG CCGCCAGACA GAAACTACAG CTAGAGAAGG TCACGGCTGA GGCCAAGATC 
AAGAAACTGG AGGATGACAT CTTGGTTATG GATGATCAGA ACAGTAAACT TTCAAAAGAA 
CGAAAACTCC TTGAAGAGAG GGTCAGCGAC TTGACAACCA ACCTAGCAGA AGAGGAAGAA 
AAGGCTAAAA ACCTCACAAA GCTGAAGAGC AAGCATGAGT CTATGATCTC AGAGCTGGAG 
GTGAGGCTGA AGAAAGAGGA GAAGAGCCGG CAGGAGCTGG AGAAACTCAA GAGGAAACTG 
GAGGGTGATG CCAGTGACTT CCATGAGCAG ATCGCTGACT TGCAGGCCCA GATTGCAGAG 
CTCAAGATGC AGCTGGCAAA GAAAGAGGAA GAGCTACAGG CAGCTCTAGC CAGGCTTGAT 
GAAGAGATCG CCCAGAAAAA CAATGCCCTA AAGAAGATTC GCGAGCTAGA GGGCCATATC 
TCAGACCTAC AAGAGGACCT AGACTCAGAG CGGGCTGCCA GGAACAAGGC CGAGAAGCAG 
AAGCGAGACC TGGGGGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA TACGCTGGAC 
AGCACAGCTA CCCAGCAGGA GCTCAGAGCC AAGAGGGAAC AGGAGGTGAC AGTGCTGAAG 
AAGGCCCTGG ATGAGGAGAC GCGGTCCCAT GAGGCCCAGG TCCAGGAGAT GAGGCAGAAG 
CACACACAGG CAGTGGAGGA ACTCACAGAG CAGCTGGAGC AGTTCAAAAG GGCCAAGGCA 
AACCTGGACA AAAGCAAGCA GACACTGGAG AAGGAGAACG CGGACCTGGC TGGGGAGCTG 
CGTGTCCTGG GCCAGGCGAA GCAGGAGGTG GAACACAAGA AGAAGAAGCT GGAGGTGCAG 
CTGCAGGATC TGCAGTCCAA GTGCAGTGAT GGGGAGCGTG CCCGGGCTGA GCTCAGTGAC 
AAGGTCCACA AGCTACAGAA TGAAGTGGAG AGTGTCACTG GCATGCTCAA TGAGGCAGAG 
GGCAAAGCCA TCAAACTGGC CAAAGATGTG GCTTCCCTTG GATCCCAGCT TCAGGACACC 
CAAGAGCTGC TCCAAGAAGA AACCCGGCAG AAGCTCAATG TGTCTACCAA GCTGCGTCAG 
TTGGAAGATG AAAGGAACAG CCTGCAGGAC CAGCTGGATG AGGAGATGGA GGCTAAGCAA 
AACCTGGAGC GCCATGTCTC AACACTGAAC ATTCAGCTCT CAGACTCTAA GAAGAAGCTG 
CAGGACTTTG CAAGTACCAT CGAGGTCATG GAGGAGGGGA AGAAGAGGTT ACAGAAAGAG 
ATGGAGGGCC TCAGCCAGCA GTATGAGGAG AAGGCGGCTG CCTATGACAA ACTGGAGAAA 
ACCAAGAACA GGCTCCAGCA GGAGCTGGAT GACCTGGTCG TGGACTTGGA CAACCAGCGG 
CAACTGGTAT CCAATCTGGA AAAGAAGCAG AAGAAATTTG ACCAGTTGTT AGCTGAGGAG 
AAGAACATCT CCTCCAAGTA TGCGGATGAG AGAGACCGAG CTGAAGCAGA GGCCAGGGAA 
AAGGAGACAA AGGCTTTGTC TCTAGCCCGG GCCCTGGAGG AAGCCCTGGA AGCCAAAGAA 
GAGCTGGAGA GGACCAACAA GATGCTCAAA GCTGAGATGG AAGACCTGGT CAGCTCCAAG 
GATGATGTAG GCAAGAACGT GCATGAACTG GAGAAGTCCA AGCGTGCCTT GGAGACCCAG 
ATGGAAGAGA TGAAAACCCA GCTGGAGGAG TTGGAGGATG AGCTGCAGGC CACTGAGGAT 
GCCAAGCTGC GGCTAGAGGT CAACATGCAG GCCCTCAAGG GCCAGTTTGA ACGCGATCTC 
CAGGCTCGGG ATGAACAGAA TGAGGAGAAG AGGAGGCAGC TACAGCGGCA GCTGCACGAG 
TATGAGACAG AACTGGAAGA TGAACGGAAG CAGAGAGCTC TGGCGGCGGC AGCTAAGAAG 
AAGCTGGAAG GGGACCTAAA AGACCTAGAG CTCCAGGCTG ACTCAGCCAT CAAAGGGAGG 
GAGGAAGCCA TCAAGCAGCT TCGAAAACTG CAGGCTCAGA TGAAGGACTT CCAAAGAGAG 
CTGGATGATG CCCGTGCCTC CAGGGATGAG ATCTTTGCCA CCTCAAAAGA GAATGAGAAG 
AAAGCCAAGA GTCTGGAGGC AGACCTCATG CAGCTCCAAG AGGACCTGGC AGCAGCTGAG 
AGAGCTCGCA AGCAAGCTGA CCTGGAGAAG GAGGAGCTGG CCGAGGAGCT GGCTAGCAGC 
TTGTCAGGAA GGAATACACT GCAGGATGAG AAGCGCCGCC TGGAGGCAAG GATCGCCCAA 
CTAGAGGAGG AGCTGGAGGA AGAGCAGGGC AACATGGAGG CCATGAGTGA TAGAGTACGC 
AAGGCCACAC TGCAGGCTGA GCAACTGAGC AATGAGCTGG CCACAGAACG CAGCACGGCT 
CAGAAGAATG AGAGCGCACG GCAACAGCTG GAGCGCCAGA ACAAGGAACT GCGAAGCAAG 
TTGCAGGAGG TAGAAGGTGC TGTCAAAGCC AAGCTCAAGT CCACTGTTGC GGCGCTGGAG 
GCCAAGATTG CACAGCTGGA GGAGCAGGTT GAACAGGAGG CCAGAGAGAA ACAGGCGGCC 
ACCAAGTCGC TGAAGCAAAA GGACAAGAAG CTAAAGGAGG TCCTGCTGCA GGTGGAGGAT 
GAGCGCAAGA TGGCAGAGCA GTACAAGGAG CAGGCAGAGA AAGGAAACAC CAAGGTCAAG 
CAGCTGAAGA GGCAGCTGGA AGAGGCAGAG GAGGAGTCCC AGCGCATCAA CGCCAACCGC 
AGGAAGCTGC AGCGGGAGCT AGATGAGGCC ACAGAGAGCA ATGAGGCCAT GGGCCGTGAG 
GTGAACGCCC TCAAGAGCAA ACTCAGGAGA GGAAACGAGG CTTCATTTGT TCCTTCCAGA 
AGGGCTGGGG GCCGTAGAGT TATTGAAAAC ACAGATGGTT CTGAAGAAGA AATGGACGCT 
CGGGACTCAG ACTTCAATGG AACCAAAGCC AGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521908.1
CDS	232..3930
Translation	MDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQLQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQKLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDARDSDFNGTKASE

Target ORF information:

RefSeq Version	XM_006521845.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521845.1

ORF Insert Sequence:

ATGGATGGAA AGCAAGCCTG CATTCTCATG ATCAAAGCCC TCGAACTTGA CCCTAACCTG 
TACAGGATTG GGCAGAGCAA AATCTTCTTC CGAACGGGGG TCCTGGCCCA CCTAGAGGAG 
GAACGAGACC TGAAAATTAC TGATGTCATC ATGGCCTTCC AGGCAATGTG TCGTGGCTAC 
CTTGCCAGAA AGGCCTTCAC CAAGAGGCAG CAACAGCTGA CAGCCATGAA GGTGATCCAG 
AGGAACTGCG CTGCCTACCT TAAGCTCCGC AACTGGCAAT GGTGGCGGCT CTTCACCAAA 
GTAAAGCCAT TGCTCCAGGT GACACGGCAG GAGGAGGAGA TGCAGGCCAA GGAGGAGGAG 
ATGCAAAAGA TCAAGGAGCG GCAGCAGAAG GCAGAGACTG AGTTGAAGGA GCTGGAGCAG 
AAGCACACTC AGCTGGCTGA GGAGAAGACT CTGCTGCAGG AGCAGTTGCA GGCAGAGACA 
GAGCTGTATG CTGAGGCTGA GGAGATGCGG GTCCGGTTGG CAGCCAAGAA GCAGGAACTG 
GAGGAGATCC TACATGAGAT GGAGGCCCGC CTGGAGGAAG AGGAAGACCG GGGCCAGCAA 
CTACAGGCTG AGAGGAAGAA GATGGCTCAG CAGATGCTAG ACCTGGAGGA GCAACTGGAG 
GAGGAAGAGG CCGCCAGACA GAAACTACAG CTAGAGAAGG TCACGGCTGA GGCCAAGATC 
AAGAAACTGG AGGATGACAT CTTGGTTATG GATGATCAGA ACAGTAAACT TTCAAAAGAA 
CGAAAACTCC TTGAAGAGAG GGTCAGCGAC TTGACAACCA ACCTAGCAGA AGAGGAAGAA 
AAGGCTAAAA ACCTCACAAA GCTGAAGAGC AAGCATGAGT CTATGATCTC AGAGCTGGAG 
GTGAGGCTGA AGAAAGAGGA GAAGAGCCGG CAGGAGCTGG AGAAACTCAA GAGGAAACTG 
GAGGGTGATG CCAGTGACTT CCATGAGCAG ATCGCTGACT TGCAGGCCCA GATTGCAGAG 
CTCAAGATGC AGCTGGCAAA GAAAGAGGAA GAGCTACAGG CAGCTCTAGC CAGGCTTGAT 
GAAGAGATCG CCCAGAAAAA CAATGCCCTA AAGAAGATTC GCGAGCTAGA GGGCCATATC 
TCAGACCTAC AAGAGGACCT AGACTCAGAG CGGGCTGCCA GGAACAAGGC CGAGAAGCAG 
AAGCGAGACC TGGGGGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA TACGCTGGAC 
AGCACAGCTA CCCAGCAGGA GCTCAGAGCC AAGAGGGAAC AGGAGGTGAC AGTGCTGAAG 
AAGGCCCTGG ATGAGGAGAC GCGGTCCCAT GAGGCCCAGG TCCAGGAGAT GAGGCAGAAG 
CACACACAGG CAGTGGAGGA ACTCACAGAG CAGCTGGAGC AGTTCAAAAG GGCCAAGGCA 
AACCTGGACA AAAGCAAGCA GACACTGGAG AAGGAGAACG CGGACCTGGC TGGGGAGCTG 
CGTGTCCTGG GCCAGGCGAA GCAGGAGGTG GAACACAAGA AGAAGAAGCT GGAGGTGCAG 
CTGCAGGATC TGCAGTCCAA GTGCAGTGAT GGGGAGCGTG CCCGGGCTGA GCTCAGTGAC 
AAGGTCCACA AGCTACAGAA TGAAGTGGAG AGTGTCACTG GCATGCTCAA TGAGGCAGAG 
GGCAAAGCCA TCAAACTGGC CAAAGATGTG GCTTCCCTTG GATCCCAGCT TCAGGACACC 
CAAGAGCTGC TCCAAGAAGA AACCCGGCAG AAGCTCAATG TGTCTACCAA GCTGCGTCAG 
TTGGAAGATG AAAGGAACAG CCTGCAGGAC CAGCTGGATG AGGAGATGGA GGCTAAGCAA 
AACCTGGAGC GCCATGTCTC AACACTGAAC ATTCAGCTCT CAGACTCTAA GAAGAAGCTG 
CAGGACTTTG CAAGTACCAT CGAGGTCATG GAGGAGGGGA AGAAGAGGTT ACAGAAAGAG 
ATGGAGGGCC TCAGCCAGCA GTATGAGGAG AAGGCGGCTG CCTATGACAA ACTGGAGAAA 
ACCAAGAACA GGCTCCAGCA GGAGCTGGAT GACCTGGTCG TGGACTTGGA CAACCAGCGG 
CAACTGGTAT CCAATCTGGA AAAGAAGCAG AAGAAATTTG ACCAGTTGTT AGCTGAGGAG 
AAGAACATCT CCTCCAAGTA TGCGGATGAG AGAGACCGAG CTGAAGCAGA GGCCAGGGAA 
AAGGAGACAA AGGCTTTGTC TCTAGCCCGG GCCCTGGAGG AAGCCCTGGA AGCCAAAGAA 
GAGCTGGAGA GGACCAACAA GATGCTCAAA GCTGAGATGG AAGACCTGGT CAGCTCCAAG 
GATGATGTAG GCAAGAACGT GCATGAACTG GAGAAGTCCA AGCGTGCCTT GGAGACCCAG 
ATGGAAGAGA TGAAAACCCA GCTGGAGGAG TTGGAGGATG AGCTGCAGGC CACTGAGGAT 
GCCAAGCTGC GGCTAGAGGT CAACATGCAG GCCCTCAAGG GCCAGTTTGA ACGCGATCTC 
CAGGCTCGGG ATGAACAGAA TGAGGAGAAG AGGAGGCAGC TACAGCGGCA GCTGCACGAG 
TATGAGACAG AACTGGAAGA TGAACGGAAG CAGAGAGCTC TGGCGGCGGC AGCTAAGAAG 
AAGCTGGAAG GGGACCTAAA AGACCTAGAG CTCCAGGCTG ACTCAGCCAT CAAAGGGAGG 
GAGGAAGCCA TCAAGCAGCT TCGAAAACTG CAGGCTCAGA TGAAGGACTT CCAAAGAGAG 
CTGGATGATG CCCGTGCCTC CAGGGATGAG ATCTTTGCCA CCTCAAAAGA GAATGAGAAG 
AAAGCCAAGA GTCTGGAGGC AGACCTCATG CAGCTCCAAG AGGACCTGGC AGCAGCTGAG 
AGAGCTCGCA AGCAAGCTGA CCTGGAGAAG GAGGAGCTGG CCGAGGAGCT GGCTAGCAGC 
TTGTCAGGAA GGAATACACT GCAGGATGAG AAGCGCCGCC TGGAGGCAAG GATCGCCCAA 
CTAGAGGAGG AGCTGGAGGA AGAGCAGGGC AACATGGAGG CCATGAGTGA TAGAGTACGC 
AAGGCCACAC TGCAGGCTGA GCAACTGAGC AATGAGCTGG CCACAGAACG CAGCACGGCT 
CAGAAGAATG AGAGCGCACG GCAACAGCTG GAGCGCCAGA ACAAGGAACT GCGAAGCAAG 
TTGCAGGAGG TAGAAGGTGC TGTCAAAGCC AAGCTCAAGT CCACTGTTGC GGCGCTGGAG 
GCCAAGATTG CACAGCTGGA GGAGCAGGTT GAACAGGAGG CCAGAGAGAA ACAGGCGGCC 
ACCAAGTCGC TGAAGCAAAA GGACAAGAAG CTAAAGGAGG TCCTGCTGCA GGTGGAGGAT 
GAGCGCAAGA TGGCAGAGCA GTACAAGGAG CAGGCAGAGA AAGGAAACAC CAAGGTCAAG 
CAGCTGAAGA GGCAGCTGGA AGAGGCAGAG GAGGAGTCCC AGCGCATCAA CGCCAACCGC 
AGGAAGCTGC AGCGGGAGCT AGATGAGGCC ACAGAGAGCA ATGAGGCCAT GGGCCGTGAG 
GTGAACGCCC TCAAGAGCAA ACTCAGGAGA GGAAACGAGG CTTCATTTGT TCCTTCCAGA 
AGGGCTGGGG GCCGTAGAGT TATTGAAAAC ACAGATGGTT CTGAAGAAGA AATGGACGCT 
CGGGACTCAG ACTTCAATGG AACCAAAGCC AGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh11 ( NM_013607.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038635.2 Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17533
Clone ID Related Accession (Same CDS sequence)	NM_013607.2
Accession Version	NM_013607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	myosin-11 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164291.2. On Jun 25, 2009 this sequence version replaced NM_013607.1. Transcript Variant: This variant (1) represents the longer transcript but encodes the shorter isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D85924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGGGGGAGC TGGAAAAGCA GCTTCTACAG 
GCAAACCCAA TCCTGGAGGC TTTCGGCAAT GCGAAAACCG TCAAGAACGA CAACTCCTCT 
CGCTTTGGCA AGTTCATTCG CATCAACTTC GATGTCACTG GTTACATTGT AGGTGCCAAT 
ATTGAAACAT ATCTTCTGGA AAAGTCTAGG GCTATTCGAC AGGCTAGGGA TGAGAGAACA 
TTTCACATCT TCTACTACCT GCTCGCCGGA GCCAAGGAAA AGATGAAAAG TGACCTGCTT 
TTGGAGAGCT TCAACAGCTA CACATTTTTA TCCAATGGCT TTGTGCCCAT CCCAGCTGCA 
CAAGATGATG AGATGTTCCA GGAGACACTG GAAGCCATGT CTATCATGGG CTTCAATGAA 
GAGGAACAGC TAGCCATCTT GAAGGTAGTA TCATCTGTCC TTCAGCTTGG AAACATTGTC 
TTCAAGAAGG AGCGAAACAC AGACCAGGCA TCCATGCCTG ATAACACAGC GGCTCAGAAA 
GTTTGCCACC TCGTGGGGAT TAATGTGACA GATTTCACTA GAGCCATCCT GACCCCACGT 
ATCAAAGTTG GACGGGATGT GGTGCAGAAG GCTCAGACCA AAGAACAGGC TGACTTTGCC 
ATCGAGGCCT TAGCCAAGGC CACCTATGAG CGCCTTTTCC GATGGATTCT CAGCCGTGTA 
AACAAGGCCT TGGACAAGAC CCATCGGCAG GGGGCCTCCT TCCTGGGCAT TCTGGATATT 
GCTGGGTTTG AAATCTTTGA GGTAAACTCC TTCGAGCAGC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTGTT CAACCACACG ATGTTCATCC TGGAGCAGGA AGAGTACCAG 
CGAGAGGGCA TCGAGTGGAA CTTCATCGAC TTCGGCCTGG ACCTGCAGCC TTGTATTGAG 
CTGATTGAGC GGCCGAACAA CCCTCCCGGT GTGCTGGCCC TGCTGGATGA AGAATGCTGG 
TTCCCCAAAG CTACAGACAA GTCTTTTGTG GAGAAGCTAT GCTCAGAGCA GGGAAATCAC 
CCCAAATTTC AGAAGCCCAA GCAGCTAAAG GACAAAACAG AGTTCTCCAT CATCCACTAT 
GCTGGGAAGG TGGACTACAA TGCAAGTGCC TGGCTGACCA AGAACATGGA CCCGCTAAAT 
GACAATGTGA CATCACTCCT CAATGCCTCC TCTGACAAGT TTGTGGCTGA CCTGTGGAAG 
GATGTGGACC GCATTGTGGG GCTGGACCAG ATGGCCAAGA TGACTGAGAG CTCACTGCCC 
AGTGCCTCCA AGACCAAAAA GGGCATGTTC CGCACCGTGG GCCAGCTCTA CAAAGAGCAG 
TTGGGGAAAC TGATGACTAC ACTGCGCAAT ACCACGCCTA ACTTTGTGCG CTGCATCATC 
CCCAACCATG AGAAGAGGTC TGGCAAGCTG GATGCATTTC TAGTGCTGGA ACAGCTGCGC 
TGCAACGGTG TGTTGGAAGG CATCCGCATC TGCCGTCAGG GCTTCCCCAA CAGGATTGTC 
TTCCAAGAGT TCCGGCAACG CTATGAGATC CTGGCAGCGA ACGCCATCCC CAAAGGCTTC 
ATGGATGGAA AGCAAGCCTG CATTCTCATG ATCAAAGCCC TCGAACTTGA CCCTAACCTG 
TACAGGATTG GGCAGAGCAA AATCTTCTTC CGAACGGGGG TCCTGGCCCA CCTAGAGGAG 
GAACGAGACC TGAAAATTAC TGATGTCATC ATGGCCTTCC AGGCAATGTG TCGTGGCTAC 
CTTGCCAGAA AGGCCTTCAC CAAGAGGCAG CAACAGCTGA CAGCCATGAA GGTGATCCAG 
AGGAACTGCG CTGCCTACCT TAAGCTCCGC AACTGGCAAT GGTGGCGGCT CTTCACCAAA 
GTAAAGCCAT TGCTCCAGGT GACACGGCAG GAGGAGGAGA TGCAGGCCAA GGAGGAGGAG 
ATGCAAAAGA TCAAGGAGCG GCAGCAGAAG GCAGAGACTG AGTTGAAGGA GCTGGAGCAG 
AAGCACACTC AGCTGGCTGA GGAGAAGACT CTGCTGCAGG AGCAGTTGCA GGCAGAGACA 
GAGCTGTATG CTGAGGCTGA GGAGATGCGG GTCCGGTTGG CAGCCAAGAA GCAGGAACTG 
GAGGAGATCC TACATGAGAT GGAGGCCCGC CTGGAGGAAG AGGAAGACCG GGGCCAGCAA 
CTACAGGCTG AGAGGAAGAA GATGGCTCAG CAGATGCTAG ACCTGGAGGA GCAACTGGAG 
GAGGAAGAGG CCGCCAGACA GAAACTACAG CTAGAGAAGG TCACGGCTGA GGCCAAGATC 
AAGAAACTGG AGGATGACAT CTTGGTTATG GATGATCAGA ACAGTAAACT TTCAAAAGAA 
CGAAAACTCC TTGAAGAGAG GGTCAGCGAC TTGACAACCA ACCTAGCAGA AGAGGAAGAA 
AAGGCTAAAA ACCTCACAAA GCTGAAGAGC AAGCATGAGT CTATGATCTC AGAGCTGGAG 
GTGAGGCTGA AGAAAGAGGA GAAGAGCCGG CAGGAGCTGG AGAAACTCAA GAGGAAACTG 
GAGGGTGATG CCAGTGACTT CCATGAGCAG ATCGCTGACT TGCAGGCCCA GATTGCAGAG 
CTCAAGATGC AGCTGGCAAA GAAAGAGGAA GAGCTACAGG CAGCTCTAGC CAGGCTTGAT 
GAAGAGATCG CCCAGAAAAA CAATGCCCTA AAGAAGATTC GCGAGCTAGA GGGCCATATC 
TCAGACCTAC AAGAGGACCT AGACTCAGAG CGGGCTGCCA GGAACAAGGC CGAGAAGCAG 
AAGCGAGACC TGGGGGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA TACGCTGGAC 
AGCACAGCTA CCCAGCAGGA GCTCAGAGCC AAGAGGGAAC AGGAGGTGAC AGTGCTGAAG 
AAGGCCCTGG ATGAGGAGAC GCGGTCCCAT GAGGCCCAGG TCCAGGAGAT GAGGCAGAAG 
CACACACAGG CAGTGGAGGA ACTCACAGAG CAGCTGGAGC AGTTCAAAAG GGCCAAGGCA 
AACCTGGACA AAAGCAAGCA GACACTGGAG AAGGAGAACG CGGACCTGGC TGGGGAGCTG 
CGTGTCCTGG GCCAGGCGAA GCAGGAGGTG GAACACAAGA AGAAGAAGCT GGAGGTGCAG 
CTGCAGGATC TGCAGTCCAA GTGCAGTGAT GGGGAGCGTG CCCGGGCTGA GCTCAGTGAC 
AAGGTCCACA AGCTACAGAA TGAAGTGGAG AGTGTCACTG GCATGCTCAA TGAGGCAGAG 
GGCAAAGCCA TCAAACTGGC CAAAGATGTG GCTTCCCTTG GATCCCAGCT TCAGGACACC 
CAAGAGCTGC TCCAAGAAGA AACCCGGCAG AAGCTCAATG TGTCTACCAA GCTGCGTCAG 
TTGGAAGATG AAAGGAACAG CCTGCAGGAC CAGCTGGATG AGGAGATGGA GGCTAAGCAA 
AACCTGGAGC GCCATGTCTC AACACTGAAC ATTCAGCTCT CAGACTCTAA GAAGAAGCTG 
CAGGACTTTG CAAGTACCAT CGAGGTCATG GAGGAGGGGA AGAAGAGGTT ACAGAAAGAG 
ATGGAGGGCC TCAGCCAGCA GTATGAGGAG AAGGCGGCTG CCTATGACAA ACTGGAGAAA 
ACCAAGAACA GGCTCCAGCA GGAGCTGGAT GACCTGGTCG TGGACTTGGA CAACCAGCGG 
CAACTGGTAT CCAATCTGGA AAAGAAGCAG AAGAAATTTG ACCAGTTGTT AGCTGAGGAG 
AAGAACATCT CCTCCAAGTA TGCGGATGAG AGAGACCGAG CTGAAGCAGA GGCCAGGGAA 
AAGGAGACAA AGGCTTTGTC TCTAGCCCGG GCCCTGGAGG AAGCCCTGGA AGCCAAAGAA 
GAGCTGGAGA GGACCAACAA GATGCTCAAA GCTGAGATGG AAGACCTGGT CAGCTCCAAG 
GATGATGTAG GCAAGAACGT GCATGAACTG GAGAAGTCCA AGCGTGCCTT GGAGACCCAG 
ATGGAAGAGA TGAAAACCCA GCTGGAGGAG TTGGAGGATG AGCTGCAGGC CACTGAGGAT 
GCCAAGCTGC GGCTAGAGGT CAACATGCAG GCCCTCAAGG GCCAGTTTGA ACGCGATCTC 
CAGGCTCGGG ATGAACAGAA TGAGGAGAAG AGGAGGCAGC TACAGCGGCA GCTGCACGAG 
TATGAGACAG AACTGGAAGA TGAACGGAAG CAGAGAGCTC TGGCGGCGGC AGCTAAGAAG 
AAGCTGGAAG GGGACCTAAA AGACCTAGAG CTCCAGGCTG ACTCAGCCAT CAAAGGGAGG 
GAGGAAGCCA TCAAGCAGCT TCGAAAACTG CAGGCTCAGA TGAAGGACTT CCAAAGAGAG 
CTGGATGATG CCCGTGCCTC CAGGGATGAG ATCTTTGCCA CCTCAAAAGA GAATGAGAAG 
AAAGCCAAGA GTCTGGAGGC AGACCTCATG CAGCTCCAAG AGGACCTGGC AGCAGCTGAG 
AGAGCTCGCA AGCAAGCTGA CCTGGAGAAG GAGGAGCTGG CCGAGGAGCT GGCTAGCAGC 
TTGTCAGGAA GGAATACACT GCAGGATGAG AAGCGCCGCC TGGAGGCAAG GATCGCCCAA 
CTAGAGGAGG AGCTGGAGGA AGAGCAGGGC AACATGGAGG CCATGAGTGA TAGAGTACGC 
AAGGCCACAC TGCAGGCTGA GCAACTGAGC AATGAGCTGG CCACAGAACG CAGCACGGCT 
CAGAAGAATG AGAGCGCACG GCAACAGCTG GAGCGCCAGA ACAAGGAACT GCGAAGCAAG 
TTGCAGGAGG TAGAAGGTGC TGTCAAAGCC AAGCTCAAGT CCACTGTTGC GGCGCTGGAG 
GCCAAGATTG CACAGCTGGA GGAGCAGGTT GAACAGGAGG CCAGAGAGAA ACAGGCGGCC 
ACCAAGTCGC TGAAGCAAAA GGACAAGAAG CTAAAGGAGG TCCTGCTGCA GGTGGAGGAT 
GAGCGCAAGA TGGCAGAGCA GTACAAGGAG CAGGCAGAGA AAGGAAACAC CAAGGTCAAG 
CAGCTGAAGA GGCAGCTGGA AGAGGCAGAG GAGGAGTCCC AGCGCATCAA CGCCAACCGC 
AGGAAGCTGC AGCGGGAGCT AGATGAGGCC ACAGAGAGCA ATGAGGCCAT GGGCCGTGAG 
GTGAACGCCC TCAAGAGCAA ACTCAGAGGG CCCCCCCCAC AGGAAACTTC GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038635.2
CDS	150..5966
Translation	MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSITGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFAIEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQLQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQKLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRGPPPQETSQ

Target ORF information:

RefSeq Version	NM_013607.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013607.2

ORF Insert Sequence:

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGGGGGAGC TGGAAAAGCA GCTTCTACAG 
GCAAACCCAA TCCTGGAGGC TTTCGGCAAT GCGAAAACCG TCAAGAACGA CAACTCCTCT 
CGCTTTGGCA AGTTCATTCG CATCAACTTC GATGTCACTG GTTACATTGT AGGTGCCAAT 
ATTGAAACAT ATCTTCTGGA AAAGTCTAGG GCTATTCGAC AGGCTAGGGA TGAGAGAACA 
TTTCACATCT TCTACTACCT GCTCGCCGGA GCCAAGGAAA AGATGAAAAG TGACCTGCTT 
TTGGAGAGCT TCAACAGCTA CACATTTTTA TCCAATGGCT TTGTGCCCAT CCCAGCTGCA 
CAAGATGATG AGATGTTCCA GGAGACACTG GAAGCCATGT CTATCATGGG CTTCAATGAA 
GAGGAACAGC TAGCCATCTT GAAGGTAGTA TCATCTGTCC TTCAGCTTGG AAACATTGTC 
TTCAAGAAGG AGCGAAACAC AGACCAGGCA TCCATGCCTG ATAACACAGC GGCTCAGAAA 
GTTTGCCACC TCGTGGGGAT TAATGTGACA GATTTCACTA GAGCCATCCT GACCCCACGT 
ATCAAAGTTG GACGGGATGT GGTGCAGAAG GCTCAGACCA AAGAACAGGC TGACTTTGCC 
ATCGAGGCCT TAGCCAAGGC CACCTATGAG CGCCTTTTCC GATGGATTCT CAGCCGTGTA 
AACAAGGCCT TGGACAAGAC CCATCGGCAG GGGGCCTCCT TCCTGGGCAT TCTGGATATT 
GCTGGGTTTG AAATCTTTGA GGTAAACTCC TTCGAGCAGC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTGTT CAACCACACG ATGTTCATCC TGGAGCAGGA AGAGTACCAG 
CGAGAGGGCA TCGAGTGGAA CTTCATCGAC TTCGGCCTGG ACCTGCAGCC TTGTATTGAG 
CTGATTGAGC GGCCGAACAA CCCTCCCGGT GTGCTGGCCC TGCTGGATGA AGAATGCTGG 
TTCCCCAAAG CTACAGACAA GTCTTTTGTG GAGAAGCTAT GCTCAGAGCA GGGAAATCAC 
CCCAAATTTC AGAAGCCCAA GCAGCTAAAG GACAAAACAG AGTTCTCCAT CATCCACTAT 
GCTGGGAAGG TGGACTACAA TGCAAGTGCC TGGCTGACCA AGAACATGGA CCCGCTAAAT 
GACAATGTGA CATCACTCCT CAATGCCTCC TCTGACAAGT TTGTGGCTGA CCTGTGGAAG 
GATGTGGACC GCATTGTGGG GCTGGACCAG ATGGCCAAGA TGACTGAGAG CTCACTGCCC 
AGTGCCTCCA AGACCAAAAA GGGCATGTTC CGCACCGTGG GCCAGCTCTA CAAAGAGCAG 
TTGGGGAAAC TGATGACTAC ACTGCGCAAT ACCACGCCTA ACTTTGTGCG CTGCATCATC 
CCCAACCATG AGAAGAGGTC TGGCAAGCTG GATGCATTTC TAGTGCTGGA ACAGCTGCGC 
TGCAACGGTG TGTTGGAAGG CATCCGCATC TGCCGTCAGG GCTTCCCCAA CAGGATTGTC 
TTCCAAGAGT TCCGGCAACG CTATGAGATC CTGGCAGCGA ACGCCATCCC CAAAGGCTTC 
ATGGATGGAA AGCAAGCCTG CATTCTCATG ATCAAAGCCC TCGAACTTGA CCCTAACCTG 
TACAGGATTG GGCAGAGCAA AATCTTCTTC CGAACGGGGG TCCTGGCCCA CCTAGAGGAG 
GAACGAGACC TGAAAATTAC TGATGTCATC ATGGCCTTCC AGGCAATGTG TCGTGGCTAC 
CTTGCCAGAA AGGCCTTCAC CAAGAGGCAG CAACAGCTGA CAGCCATGAA GGTGATCCAG 
AGGAACTGCG CTGCCTACCT TAAGCTCCGC AACTGGCAAT GGTGGCGGCT CTTCACCAAA 
GTAAAGCCAT TGCTCCAGGT GACACGGCAG GAGGAGGAGA TGCAGGCCAA GGAGGAGGAG 
ATGCAAAAGA TCAAGGAGCG GCAGCAGAAG GCAGAGACTG AGTTGAAGGA GCTGGAGCAG 
AAGCACACTC AGCTGGCTGA GGAGAAGACT CTGCTGCAGG AGCAGTTGCA GGCAGAGACA 
GAGCTGTATG CTGAGGCTGA GGAGATGCGG GTCCGGTTGG CAGCCAAGAA GCAGGAACTG 
GAGGAGATCC TACATGAGAT GGAGGCCCGC CTGGAGGAAG AGGAAGACCG GGGCCAGCAA 
CTACAGGCTG AGAGGAAGAA GATGGCTCAG CAGATGCTAG ACCTGGAGGA GCAACTGGAG 
GAGGAAGAGG CCGCCAGACA GAAACTACAG CTAGAGAAGG TCACGGCTGA GGCCAAGATC 
AAGAAACTGG AGGATGACAT CTTGGTTATG GATGATCAGA ACAGTAAACT TTCAAAAGAA 
CGAAAACTCC TTGAAGAGAG GGTCAGCGAC TTGACAACCA ACCTAGCAGA AGAGGAAGAA 
AAGGCTAAAA ACCTCACAAA GCTGAAGAGC AAGCATGAGT CTATGATCTC AGAGCTGGAG 
GTGAGGCTGA AGAAAGAGGA GAAGAGCCGG CAGGAGCTGG AGAAACTCAA GAGGAAACTG 
GAGGGTGATG CCAGTGACTT CCATGAGCAG ATCGCTGACT TGCAGGCCCA GATTGCAGAG 
CTCAAGATGC AGCTGGCAAA GAAAGAGGAA GAGCTACAGG CAGCTCTAGC CAGGCTTGAT 
GAAGAGATCG CCCAGAAAAA CAATGCCCTA AAGAAGATTC GCGAGCTAGA GGGCCATATC 
TCAGACCTAC AAGAGGACCT AGACTCAGAG CGGGCTGCCA GGAACAAGGC CGAGAAGCAG 
AAGCGAGACC TGGGGGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA TACGCTGGAC 
AGCACAGCTA CCCAGCAGGA GCTCAGAGCC AAGAGGGAAC AGGAGGTGAC AGTGCTGAAG 
AAGGCCCTGG ATGAGGAGAC GCGGTCCCAT GAGGCCCAGG TCCAGGAGAT GAGGCAGAAG 
CACACACAGG CAGTGGAGGA ACTCACAGAG CAGCTGGAGC AGTTCAAAAG GGCCAAGGCA 
AACCTGGACA AAAGCAAGCA GACACTGGAG AAGGAGAACG CGGACCTGGC TGGGGAGCTG 
CGTGTCCTGG GCCAGGCGAA GCAGGAGGTG GAACACAAGA AGAAGAAGCT GGAGGTGCAG 
CTGCAGGATC TGCAGTCCAA GTGCAGTGAT GGGGAGCGTG CCCGGGCTGA GCTCAGTGAC 
AAGGTCCACA AGCTACAGAA TGAAGTGGAG AGTGTCACTG GCATGCTCAA TGAGGCAGAG 
GGCAAAGCCA TCAAACTGGC CAAAGATGTG GCTTCCCTTG GATCCCAGCT TCAGGACACC 
CAAGAGCTGC TCCAAGAAGA AACCCGGCAG AAGCTCAATG TGTCTACCAA GCTGCGTCAG 
TTGGAAGATG AAAGGAACAG CCTGCAGGAC CAGCTGGATG AGGAGATGGA GGCTAAGCAA 
AACCTGGAGC GCCATGTCTC AACACTGAAC ATTCAGCTCT CAGACTCTAA GAAGAAGCTG 
CAGGACTTTG CAAGTACCAT CGAGGTCATG GAGGAGGGGA AGAAGAGGTT ACAGAAAGAG 
ATGGAGGGCC TCAGCCAGCA GTATGAGGAG AAGGCGGCTG CCTATGACAA ACTGGAGAAA 
ACCAAGAACA GGCTCCAGCA GGAGCTGGAT GACCTGGTCG TGGACTTGGA CAACCAGCGG 
CAACTGGTAT CCAATCTGGA AAAGAAGCAG AAGAAATTTG ACCAGTTGTT AGCTGAGGAG 
AAGAACATCT CCTCCAAGTA TGCGGATGAG AGAGACCGAG CTGAAGCAGA GGCCAGGGAA 
AAGGAGACAA AGGCTTTGTC TCTAGCCCGG GCCCTGGAGG AAGCCCTGGA AGCCAAAGAA 
GAGCTGGAGA GGACCAACAA GATGCTCAAA GCTGAGATGG AAGACCTGGT CAGCTCCAAG 
GATGATGTAG GCAAGAACGT GCATGAACTG GAGAAGTCCA AGCGTGCCTT GGAGACCCAG 
ATGGAAGAGA TGAAAACCCA GCTGGAGGAG TTGGAGGATG AGCTGCAGGC CACTGAGGAT 
GCCAAGCTGC GGCTAGAGGT CAACATGCAG GCCCTCAAGG GCCAGTTTGA ACGCGATCTC 
CAGGCTCGGG ATGAACAGAA TGAGGAGAAG AGGAGGCAGC TACAGCGGCA GCTGCACGAG 
TATGAGACAG AACTGGAAGA TGAACGGAAG CAGAGAGCTC TGGCGGCGGC AGCTAAGAAG 
AAGCTGGAAG GGGACCTAAA AGACCTAGAG CTCCAGGCTG ACTCAGCCAT CAAAGGGAGG 
GAGGAAGCCA TCAAGCAGCT TCGAAAACTG CAGGCTCAGA TGAAGGACTT CCAAAGAGAG 
CTGGATGATG CCCGTGCCTC CAGGGATGAG ATCTTTGCCA CCTCAAAAGA GAATGAGAAG 
AAAGCCAAGA GTCTGGAGGC AGACCTCATG CAGCTCCAAG AGGACCTGGC AGCAGCTGAG 
AGAGCTCGCA AGCAAGCTGA CCTGGAGAAG GAGGAGCTGG CCGAGGAGCT GGCTAGCAGC 
TTGTCAGGAA GGAATACACT GCAGGATGAG AAGCGCCGCC TGGAGGCAAG GATCGCCCAA 
CTAGAGGAGG AGCTGGAGGA AGAGCAGGGC AACATGGAGG CCATGAGTGA TAGAGTACGC 
AAGGCCACAC TGCAGGCTGA GCAACTGAGC AATGAGCTGG CCACAGAACG CAGCACGGCT 
CAGAAGAATG AGAGCGCACG GCAACAGCTG GAGCGCCAGA ACAAGGAACT GCGAAGCAAG 
TTGCAGGAGG TAGAAGGTGC TGTCAAAGCC AAGCTCAAGT CCACTGTTGC GGCGCTGGAG 
GCCAAGATTG CACAGCTGGA GGAGCAGGTT GAACAGGAGG CCAGAGAGAA ACAGGCGGCC 
ACCAAGTCGC TGAAGCAAAA GGACAAGAAG CTAAAGGAGG TCCTGCTGCA GGTGGAGGAT 
GAGCGCAAGA TGGCAGAGCA GTACAAGGAG CAGGCAGAGA AAGGAAACAC CAAGGTCAAG 
CAGCTGAAGA GGCAGCTGGA AGAGGCAGAG GAGGAGTCCC AGCGCATCAA CGCCAACCGC 
AGGAAGCTGC AGCGGGAGCT AGATGAGGCC ACAGAGAGCA ATGAGGCCAT GGGCCGTGAG 
GTGAACGCCC TCAAGAGCAA ACTCAGAGGG CCCCCCCCAC AGGAAACTTC GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh11 ( NM_001161775.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155247.1 Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant 2, mRNA.

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CloneID	OMu17607
Clone ID Related Accession (Same CDS sequence)	NM_001161775.1
Accession Version	NM_001161775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	myosin-11 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164291.2. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2) has a longer and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK173045.1, D85923.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGGGGGAGC TGGAAAAGCA GCTTCTACAG 
GCAAACCCAA TCCTGGAGGC TTTCGGCAAT GCGAAAACCG TCAAGAACGA CAACTCCTCT 
CGCTTTGGCA AGTTCATTCG CATCAACTTC GATGTCACTG GTTACATTGT AGGTGCCAAT 
ATTGAAACAT ATCTTCTGGA AAAGTCTAGG GCTATTCGAC AGGCTAGGGA TGAGAGAACA 
TTTCACATCT TCTACTACCT GCTCGCCGGA GCCAAGGAAA AGATGAAAAG TGACCTGCTT 
TTGGAGAGCT TCAACAGCTA CACATTTTTA TCCAATGGCT TTGTGCCCAT CCCAGCTGCA 
CAAGATGATG AGATGTTCCA GGAGACACTG GAAGCCATGT CTATCATGGG CTTCAATGAA 
GAGGAACAGC TAGCCATCTT GAAGGTAGTA TCATCTGTCC TTCAGCTTGG AAACATTGTC 
TTCAAGAAGG AGCGAAACAC AGACCAGGCA TCCATGCCTG ATAACACAGC GGCTCAGAAA 
GTTTGCCACC TCGTGGGGAT TAATGTGACA GATTTCACTA GAGCCATCCT GACCCCACGT 
ATCAAAGTTG GACGGGATGT GGTGCAGAAG GCTCAGACCA AAGAACAGGC TGACTTTGCC 
ATCGAGGCCT TAGCCAAGGC CACCTATGAG CGCCTTTTCC GATGGATTCT CAGCCGTGTA 
AACAAGGCCT TGGACAAGAC CCATCGGCAG GGGGCCTCCT TCCTGGGCAT TCTGGATATT 
GCTGGGTTTG AAATCTTTGA GGTAAACTCC TTCGAGCAGC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTGTT CAACCACACG ATGTTCATCC TGGAGCAGGA AGAGTACCAG 
CGAGAGGGCA TCGAGTGGAA CTTCATCGAC TTCGGCCTGG ACCTGCAGCC TTGTATTGAG 
CTGATTGAGC GGCCGAACAA CCCTCCCGGT GTGCTGGCCC TGCTGGATGA AGAATGCTGG 
TTCCCCAAAG CTACAGACAA GTCTTTTGTG GAGAAGCTAT GCTCAGAGCA GGGAAATCAC 
CCCAAATTTC AGAAGCCCAA GCAGCTAAAG GACAAAACAG AGTTCTCCAT CATCCACTAT 
GCTGGGAAGG TGGACTACAA TGCAAGTGCC TGGCTGACCA AGAACATGGA CCCGCTAAAT 
GACAATGTGA CATCACTCCT CAATGCCTCC TCTGACAAGT TTGTGGCTGA CCTGTGGAAG 
GATGTGGACC GCATTGTGGG GCTGGACCAG ATGGCCAAGA TGACTGAGAG CTCACTGCCC 
AGTGCCTCCA AGACCAAAAA GGGCATGTTC CGCACCGTGG GCCAGCTCTA CAAAGAGCAG 
TTGGGGAAAC TGATGACTAC ACTGCGCAAT ACCACGCCTA ACTTTGTGCG CTGCATCATC 
CCCAACCATG AGAAGAGGTC TGGCAAGCTG GATGCATTTC TAGTGCTGGA ACAGCTGCGC 
TGCAACGGTG TGTTGGAAGG CATCCGCATC TGCCGTCAGG GCTTCCCCAA CAGGATTGTC 
TTCCAAGAGT TCCGGCAACG CTATGAGATC CTGGCAGCGA ACGCCATCCC CAAAGGCTTC 
ATGGATGGAA AGCAAGCCTG CATTCTCATG ATCAAAGCCC TCGAACTTGA CCCTAACCTG 
TACAGGATTG GGCAGAGCAA AATCTTCTTC CGAACGGGGG TCCTGGCCCA CCTAGAGGAG 
GAACGAGACC TGAAAATTAC TGATGTCATC ATGGCCTTCC AGGCAATGTG TCGTGGCTAC 
CTTGCCAGAA AGGCCTTCAC CAAGAGGCAG CAACAGCTGA CAGCCATGAA GGTGATCCAG 
AGGAACTGCG CTGCCTACCT TAAGCTCCGC AACTGGCAAT GGTGGCGGCT CTTCACCAAA 
GTAAAGCCAT TGCTCCAGGT GACACGGCAG GAGGAGGAGA TGCAGGCCAA GGAGGAGGAG 
ATGCAAAAGA TCAAGGAGCG GCAGCAGAAG GCAGAGACTG AGTTGAAGGA GCTGGAGCAG 
AAGCACACTC AGCTGGCTGA GGAGAAGACT CTGCTGCAGG AGCAGTTGCA GGCAGAGACA 
GAGCTGTATG CTGAGGCTGA GGAGATGCGG GTCCGGTTGG CAGCCAAGAA GCAGGAACTG 
GAGGAGATCC TACATGAGAT GGAGGCCCGC CTGGAGGAAG AGGAAGACCG GGGCCAGCAA 
CTACAGGCTG AGAGGAAGAA GATGGCTCAG CAGATGCTAG ACCTGGAGGA GCAACTGGAG 
GAGGAAGAGG CCGCCAGACA GAAACTACAG CTAGAGAAGG TCACGGCTGA GGCCAAGATC 
AAGAAACTGG AGGATGACAT CTTGGTTATG GATGATCAGA ACAGTAAACT TTCAAAAGAA 
CGAAAACTCC TTGAAGAGAG GGTCAGCGAC TTGACAACCA ACCTAGCAGA AGAGGAAGAA 
AAGGCTAAAA ACCTCACAAA GCTGAAGAGC AAGCATGAGT CTATGATCTC AGAGCTGGAG 
GTGAGGCTGA AGAAAGAGGA GAAGAGCCGG CAGGAGCTGG AGAAACTCAA GAGGAAACTG 
GAGGGTGATG CCAGTGACTT CCATGAGCAG ATCGCTGACT TGCAGGCCCA GATTGCAGAG 
CTCAAGATGC AGCTGGCAAA GAAAGAGGAA GAGCTACAGG CAGCTCTAGC CAGGCTTGAT 
GAAGAGATCG CCCAGAAAAA CAATGCCCTA AAGAAGATTC GCGAGCTAGA GGGCCATATC 
TCAGACCTAC AAGAGGACCT AGACTCAGAG CGGGCTGCCA GGAACAAGGC CGAGAAGCAG 
AAGCGAGACC TGGGGGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA TACGCTGGAC 
AGCACAGCTA CCCAGCAGGA GCTCAGAGCC AAGAGGGAAC AGGAGGTGAC AGTGCTGAAG 
AAGGCCCTGG ATGAGGAGAC GCGGTCCCAT GAGGCCCAGG TCCAGGAGAT GAGGCAGAAG 
CACACACAGG CAGTGGAGGA ACTCACAGAG CAGCTGGAGC AGTTCAAAAG GGCCAAGGCA 
AACCTGGACA AAAGCAAGCA GACACTGGAG AAGGAGAACG CGGACCTGGC TGGGGAGCTG 
CGTGTCCTGG GCCAGGCGAA GCAGGAGGTG GAACACAAGA AGAAGAAGCT GGAGGTGCAG 
CTGCAGGATC TGCAGTCCAA GTGCAGTGAT GGGGAGCGTG CCCGGGCTGA GCTCAGTGAC 
AAGGTCCACA AGCTACAGAA TGAAGTGGAG AGTGTCACTG GCATGCTCAA TGAGGCAGAG 
GGCAAAGCCA TCAAACTGGC CAAAGATGTG GCTTCCCTTG GATCCCAGCT TCAGGACACC 
CAAGAGCTGC TCCAAGAAGA AACCCGGCAG AAGCTCAATG TGTCTACCAA GCTGCGTCAG 
TTGGAAGATG AAAGGAACAG CCTGCAGGAC CAGCTGGATG AGGAGATGGA GGCTAAGCAA 
AACCTGGAGC GCCATGTCTC AACACTGAAC ATTCAGCTCT CAGACTCTAA GAAGAAGCTG 
CAGGACTTTG CAAGTACCAT CGAGGTCATG GAGGAGGGGA AGAAGAGGTT ACAGAAAGAG 
ATGGAGGGCC TCAGCCAGCA GTATGAGGAG AAGGCGGCTG CCTATGACAA ACTGGAGAAA 
ACCAAGAACA GGCTCCAGCA GGAGCTGGAT GACCTGGTCG TGGACTTGGA CAACCAGCGG 
CAACTGGTAT CCAATCTGGA AAAGAAGCAG AAGAAATTTG ACCAGTTGTT AGCTGAGGAG 
AAGAACATCT CCTCCAAGTA TGCGGATGAG AGAGACCGAG CTGAAGCAGA GGCCAGGGAA 
AAGGAGACAA AGGCTTTGTC TCTAGCCCGG GCCCTGGAGG AAGCCCTGGA AGCCAAAGAA 
GAGCTGGAGA GGACCAACAA GATGCTCAAA GCTGAGATGG AAGACCTGGT CAGCTCCAAG 
GATGATGTAG GCAAGAACGT GCATGAACTG GAGAAGTCCA AGCGTGCCTT GGAGACCCAG 
ATGGAAGAGA TGAAAACCCA GCTGGAGGAG TTGGAGGATG AGCTGCAGGC CACTGAGGAT 
GCCAAGCTGC GGCTAGAGGT CAACATGCAG GCCCTCAAGG GCCAGTTTGA ACGCGATCTC 
CAGGCTCGGG ATGAACAGAA TGAGGAGAAG AGGAGGCAGC TACAGCGGCA GCTGCACGAG 
TATGAGACAG AACTGGAAGA TGAACGGAAG CAGAGAGCTC TGGCGGCGGC AGCTAAGAAG 
AAGCTGGAAG GGGACCTAAA AGACCTAGAG CTCCAGGCTG ACTCAGCCAT CAAAGGGAGG 
GAGGAAGCCA TCAAGCAGCT TCGAAAACTG CAGGCTCAGA TGAAGGACTT CCAAAGAGAG 
CTGGATGATG CCCGTGCCTC CAGGGATGAG ATCTTTGCCA CCTCAAAAGA GAATGAGAAG 
AAAGCCAAGA GTCTGGAGGC AGACCTCATG CAGCTCCAAG AGGACCTGGC AGCAGCTGAG 
AGAGCTCGCA AGCAAGCTGA CCTGGAGAAG GAGGAGCTGG CCGAGGAGCT GGCTAGCAGC 
TTGTCAGGAA GGAATACACT GCAGGATGAG AAGCGCCGCC TGGAGGCAAG GATCGCCCAA 
CTAGAGGAGG AGCTGGAGGA AGAGCAGGGC AACATGGAGG CCATGAGTGA TAGAGTACGC 
AAGGCCACAC TGCAGGCTGA GCAACTGAGC AATGAGCTGG CCACAGAACG CAGCACGGCT 
CAGAAGAATG AGAGCGCACG GCAACAGCTG GAGCGCCAGA ACAAGGAACT GCGAAGCAAG 
TTGCAGGAGG TAGAAGGTGC TGTCAAAGCC AAGCTCAAGT CCACTGTTGC GGCGCTGGAG 
GCCAAGATTG CACAGCTGGA GGAGCAGGTT GAACAGGAGG CCAGAGAGAA ACAGGCGGCC 
ACCAAGTCGC TGAAGCAAAA GGACAAGAAG CTAAAGGAGG TCCTGCTGCA GGTGGAGGAT 
GAGCGCAAGA TGGCAGAGCA GTACAAGGAG CAGGCAGAGA AAGGAAACAC CAAGGTCAAG 
CAGCTGAAGA GGCAGCTGGA AGAGGCAGAG GAGGAGTCCC AGCGCATCAA CGCCAACCGC 
AGGAAGCTGC AGCGGGAGCT AGATGAGGCC ACAGAGAGCA ATGAGGCCAT GGGCCGTGAG 
GTGAACGCCC TCAAGAGCAA ACTCAGGAGA GGAAACGAGG CTTCATTTGT TCCTTCCAGA 
AGGGCTGGGG GCCGTAGAGT TATTGAAAAC ACAGATGGTT CTGAAGAAGA AATGGACGCT 
CGGGACTCAG ACTTCAATGG AACCAAAGCC AGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155247.1
CDS	150..6068
Translation	MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSITGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFAIEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQLQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQKLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDARDSDFNGTKASE

Target ORF information:

RefSeq Version	NM_001161775.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161775.1

ORF Insert Sequence:

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGGGGGAGC TGGAAAAGCA GCTTCTACAG 
GCAAACCCAA TCCTGGAGGC TTTCGGCAAT GCGAAAACCG TCAAGAACGA CAACTCCTCT 
CGCTTTGGCA AGTTCATTCG CATCAACTTC GATGTCACTG GTTACATTGT AGGTGCCAAT 
ATTGAAACAT ATCTTCTGGA AAAGTCTAGG GCTATTCGAC AGGCTAGGGA TGAGAGAACA 
TTTCACATCT TCTACTACCT GCTCGCCGGA GCCAAGGAAA AGATGAAAAG TGACCTGCTT 
TTGGAGAGCT TCAACAGCTA CACATTTTTA TCCAATGGCT TTGTGCCCAT CCCAGCTGCA 
CAAGATGATG AGATGTTCCA GGAGACACTG GAAGCCATGT CTATCATGGG CTTCAATGAA 
GAGGAACAGC TAGCCATCTT GAAGGTAGTA TCATCTGTCC TTCAGCTTGG AAACATTGTC 
TTCAAGAAGG AGCGAAACAC AGACCAGGCA TCCATGCCTG ATAACACAGC GGCTCAGAAA 
GTTTGCCACC TCGTGGGGAT TAATGTGACA GATTTCACTA GAGCCATCCT GACCCCACGT 
ATCAAAGTTG GACGGGATGT GGTGCAGAAG GCTCAGACCA AAGAACAGGC TGACTTTGCC 
ATCGAGGCCT TAGCCAAGGC CACCTATGAG CGCCTTTTCC GATGGATTCT CAGCCGTGTA 
AACAAGGCCT TGGACAAGAC CCATCGGCAG GGGGCCTCCT TCCTGGGCAT TCTGGATATT 
GCTGGGTTTG AAATCTTTGA GGTAAACTCC TTCGAGCAGC TGTGCATCAA CTACACCAAC 
GAGAAGCTGC AGCAGCTGTT CAACCACACG ATGTTCATCC TGGAGCAGGA AGAGTACCAG 
CGAGAGGGCA TCGAGTGGAA CTTCATCGAC TTCGGCCTGG ACCTGCAGCC TTGTATTGAG 
CTGATTGAGC GGCCGAACAA CCCTCCCGGT GTGCTGGCCC TGCTGGATGA AGAATGCTGG 
TTCCCCAAAG CTACAGACAA GTCTTTTGTG GAGAAGCTAT GCTCAGAGCA GGGAAATCAC 
CCCAAATTTC AGAAGCCCAA GCAGCTAAAG GACAAAACAG AGTTCTCCAT CATCCACTAT 
GCTGGGAAGG TGGACTACAA TGCAAGTGCC TGGCTGACCA AGAACATGGA CCCGCTAAAT 
GACAATGTGA CATCACTCCT CAATGCCTCC TCTGACAAGT TTGTGGCTGA CCTGTGGAAG 
GATGTGGACC GCATTGTGGG GCTGGACCAG ATGGCCAAGA TGACTGAGAG CTCACTGCCC 
AGTGCCTCCA AGACCAAAAA GGGCATGTTC CGCACCGTGG GCCAGCTCTA CAAAGAGCAG 
TTGGGGAAAC TGATGACTAC ACTGCGCAAT ACCACGCCTA ACTTTGTGCG CTGCATCATC 
CCCAACCATG AGAAGAGGTC TGGCAAGCTG GATGCATTTC TAGTGCTGGA ACAGCTGCGC 
TGCAACGGTG TGTTGGAAGG CATCCGCATC TGCCGTCAGG GCTTCCCCAA CAGGATTGTC 
TTCCAAGAGT TCCGGCAACG CTATGAGATC CTGGCAGCGA ACGCCATCCC CAAAGGCTTC 
ATGGATGGAA AGCAAGCCTG CATTCTCATG ATCAAAGCCC TCGAACTTGA CCCTAACCTG 
TACAGGATTG GGCAGAGCAA AATCTTCTTC CGAACGGGGG TCCTGGCCCA CCTAGAGGAG 
GAACGAGACC TGAAAATTAC TGATGTCATC ATGGCCTTCC AGGCAATGTG TCGTGGCTAC 
CTTGCCAGAA AGGCCTTCAC CAAGAGGCAG CAACAGCTGA CAGCCATGAA GGTGATCCAG 
AGGAACTGCG CTGCCTACCT TAAGCTCCGC AACTGGCAAT GGTGGCGGCT CTTCACCAAA 
GTAAAGCCAT TGCTCCAGGT GACACGGCAG GAGGAGGAGA TGCAGGCCAA GGAGGAGGAG 
ATGCAAAAGA TCAAGGAGCG GCAGCAGAAG GCAGAGACTG AGTTGAAGGA GCTGGAGCAG 
AAGCACACTC AGCTGGCTGA GGAGAAGACT CTGCTGCAGG AGCAGTTGCA GGCAGAGACA 
GAGCTGTATG CTGAGGCTGA GGAGATGCGG GTCCGGTTGG CAGCCAAGAA GCAGGAACTG 
GAGGAGATCC TACATGAGAT GGAGGCCCGC CTGGAGGAAG AGGAAGACCG GGGCCAGCAA 
CTACAGGCTG AGAGGAAGAA GATGGCTCAG CAGATGCTAG ACCTGGAGGA GCAACTGGAG 
GAGGAAGAGG CCGCCAGACA GAAACTACAG CTAGAGAAGG TCACGGCTGA GGCCAAGATC 
AAGAAACTGG AGGATGACAT CTTGGTTATG GATGATCAGA ACAGTAAACT TTCAAAAGAA 
CGAAAACTCC TTGAAGAGAG GGTCAGCGAC TTGACAACCA ACCTAGCAGA AGAGGAAGAA 
AAGGCTAAAA ACCTCACAAA GCTGAAGAGC AAGCATGAGT CTATGATCTC AGAGCTGGAG 
GTGAGGCTGA AGAAAGAGGA GAAGAGCCGG CAGGAGCTGG AGAAACTCAA GAGGAAACTG 
GAGGGTGATG CCAGTGACTT CCATGAGCAG ATCGCTGACT TGCAGGCCCA GATTGCAGAG 
CTCAAGATGC AGCTGGCAAA GAAAGAGGAA GAGCTACAGG CAGCTCTAGC CAGGCTTGAT 
GAAGAGATCG CCCAGAAAAA CAATGCCCTA AAGAAGATTC GCGAGCTAGA GGGCCATATC 
TCAGACCTAC AAGAGGACCT AGACTCAGAG CGGGCTGCCA GGAACAAGGC CGAGAAGCAG 
AAGCGAGACC TGGGGGAGGA GCTGGAGGCA CTCAAGACGG AGCTGGAGGA TACGCTGGAC 
AGCACAGCTA CCCAGCAGGA GCTCAGAGCC AAGAGGGAAC AGGAGGTGAC AGTGCTGAAG 
AAGGCCCTGG ATGAGGAGAC GCGGTCCCAT GAGGCCCAGG TCCAGGAGAT GAGGCAGAAG 
CACACACAGG CAGTGGAGGA ACTCACAGAG CAGCTGGAGC AGTTCAAAAG GGCCAAGGCA 
AACCTGGACA AAAGCAAGCA GACACTGGAG AAGGAGAACG CGGACCTGGC TGGGGAGCTG 
CGTGTCCTGG GCCAGGCGAA GCAGGAGGTG GAACACAAGA AGAAGAAGCT GGAGGTGCAG 
CTGCAGGATC TGCAGTCCAA GTGCAGTGAT GGGGAGCGTG CCCGGGCTGA GCTCAGTGAC 
AAGGTCCACA AGCTACAGAA TGAAGTGGAG AGTGTCACTG GCATGCTCAA TGAGGCAGAG 
GGCAAAGCCA TCAAACTGGC CAAAGATGTG GCTTCCCTTG GATCCCAGCT TCAGGACACC 
CAAGAGCTGC TCCAAGAAGA AACCCGGCAG AAGCTCAATG TGTCTACCAA GCTGCGTCAG 
TTGGAAGATG AAAGGAACAG CCTGCAGGAC CAGCTGGATG AGGAGATGGA GGCTAAGCAA 
AACCTGGAGC GCCATGTCTC AACACTGAAC ATTCAGCTCT CAGACTCTAA GAAGAAGCTG 
CAGGACTTTG CAAGTACCAT CGAGGTCATG GAGGAGGGGA AGAAGAGGTT ACAGAAAGAG 
ATGGAGGGCC TCAGCCAGCA GTATGAGGAG AAGGCGGCTG CCTATGACAA ACTGGAGAAA 
ACCAAGAACA GGCTCCAGCA GGAGCTGGAT GACCTGGTCG TGGACTTGGA CAACCAGCGG 
CAACTGGTAT CCAATCTGGA AAAGAAGCAG AAGAAATTTG ACCAGTTGTT AGCTGAGGAG 
AAGAACATCT CCTCCAAGTA TGCGGATGAG AGAGACCGAG CTGAAGCAGA GGCCAGGGAA 
AAGGAGACAA AGGCTTTGTC TCTAGCCCGG GCCCTGGAGG AAGCCCTGGA AGCCAAAGAA 
GAGCTGGAGA GGACCAACAA GATGCTCAAA GCTGAGATGG AAGACCTGGT CAGCTCCAAG 
GATGATGTAG GCAAGAACGT GCATGAACTG GAGAAGTCCA AGCGTGCCTT GGAGACCCAG 
ATGGAAGAGA TGAAAACCCA GCTGGAGGAG TTGGAGGATG AGCTGCAGGC CACTGAGGAT 
GCCAAGCTGC GGCTAGAGGT CAACATGCAG GCCCTCAAGG GCCAGTTTGA ACGCGATCTC 
CAGGCTCGGG ATGAACAGAA TGAGGAGAAG AGGAGGCAGC TACAGCGGCA GCTGCACGAG 
TATGAGACAG AACTGGAAGA TGAACGGAAG CAGAGAGCTC TGGCGGCGGC AGCTAAGAAG 
AAGCTGGAAG GGGACCTAAA AGACCTAGAG CTCCAGGCTG ACTCAGCCAT CAAAGGGAGG 
GAGGAAGCCA TCAAGCAGCT TCGAAAACTG CAGGCTCAGA TGAAGGACTT CCAAAGAGAG 
CTGGATGATG CCCGTGCCTC CAGGGATGAG ATCTTTGCCA CCTCAAAAGA GAATGAGAAG 
AAAGCCAAGA GTCTGGAGGC AGACCTCATG CAGCTCCAAG AGGACCTGGC AGCAGCTGAG 
AGAGCTCGCA AGCAAGCTGA CCTGGAGAAG GAGGAGCTGG CCGAGGAGCT GGCTAGCAGC 
TTGTCAGGAA GGAATACACT GCAGGATGAG AAGCGCCGCC TGGAGGCAAG GATCGCCCAA 
CTAGAGGAGG AGCTGGAGGA AGAGCAGGGC AACATGGAGG CCATGAGTGA TAGAGTACGC 
AAGGCCACAC TGCAGGCTGA GCAACTGAGC AATGAGCTGG CCACAGAACG CAGCACGGCT 
CAGAAGAATG AGAGCGCACG GCAACAGCTG GAGCGCCAGA ACAAGGAACT GCGAAGCAAG 
TTGCAGGAGG TAGAAGGTGC TGTCAAAGCC AAGCTCAAGT CCACTGTTGC GGCGCTGGAG 
GCCAAGATTG CACAGCTGGA GGAGCAGGTT GAACAGGAGG CCAGAGAGAA ACAGGCGGCC 
ACCAAGTCGC TGAAGCAAAA GGACAAGAAG CTAAAGGAGG TCCTGCTGCA GGTGGAGGAT 
GAGCGCAAGA TGGCAGAGCA GTACAAGGAG CAGGCAGAGA AAGGAAACAC CAAGGTCAAG 
CAGCTGAAGA GGCAGCTGGA AGAGGCAGAG GAGGAGTCCC AGCGCATCAA CGCCAACCGC 
AGGAAGCTGC AGCGGGAGCT AGATGAGGCC ACAGAGAGCA ATGAGGCCAT GGGCCGTGAG 
GTGAACGCCC TCAAGAGCAA ACTCAGGAGA GGAAACGAGG CTTCATTTGT TCCTTCCAGA 
AGGGCTGGGG GCCGTAGAGT TATTGAAAAC ACAGATGGTT CTGAAGAAGA AATGGACGCT 
CGGGACTCAG ACTTCAATGG AACCAAAGCC AGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh11 ( XM_006521843.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521906.1 Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X1, mRNA.

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CloneID	OMu51914
Clone ID Related Accession (Same CDS sequence)	XM_006521843.1 , XM_006521843.2
Accession Version	XM_006521843.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	myosin-11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGGAG AGGAAACGAG 
GCTTCATTTG TTCCTTCCAG AAGGGCTGGG GGCCGTAGAG TTATTGAAAA CACAGATGGT 
TCTGAAGAAG AAATGGACGC TCGGGACTCA GACTTCAATG GAACCAAAGC CAGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521906.1
CDS	145..6084
Translation	MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSITQGPSFAYGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFAIEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQLQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQKLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDARDSDFNGTKASE

Target ORF information:

RefSeq Version	XM_006521843.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521843.1

ORF Insert Sequence:

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGGAG AGGAAACGAG 
GCTTCATTTG TTCCTTCCAG AAGGGCTGGG GGCCGTAGAG TTATTGAAAA CACAGATGGT 
TCTGAAGAAG AAATGGACGC TCGGGACTCA GACTTCAATG GAACCAAAGC CAGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh11 ( XM_006521843.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521906.1 Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X1, mRNA.

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CloneID	OMu51914
Clone ID Related Accession (Same CDS sequence)	XM_006521843.1 , XM_006521843.2
Accession Version	XM_006521843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	myosin-11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGGAG AGGAAACGAG 
GCTTCATTTG TTCCTTCCAG AAGGGCTGGG GGCCGTAGAG TTATTGAAAA CACAGATGGT 
TCTGAAGAAG AAATGGACGC TCGGGACTCA GACTTCAATG GAACCAAAGC CAGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521906.1
CDS	930..6869
Translation	MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSITQGPSFAYGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFAIEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQLQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQKLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDARDSDFNGTKASE

Target ORF information:

RefSeq Version	XM_006521843.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521843.2

ORF Insert Sequence:

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGGAG AGGAAACGAG 
GCTTCATTTG TTCCTTCCAG AAGGGCTGGG GGCCGTAGAG TTATTGAAAA CACAGATGGT 
TCTGAAGAAG AAATGGACGC TCGGGACTCA GACTTCAATG GAACCAAAGC CAGTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh11 ( XM_006521844.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521907.1 Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X2, mRNA.

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CloneID	OMu51915
Clone ID Related Accession (Same CDS sequence)	XM_006521844.2 , XM_006521844.1
Accession Version	XM_006521844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	myosin-11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGAGG GCCCCCCCCA 
CAGGAAACTT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521907.1
CDS	930..6767
Translation	MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSITQGPSFAYGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFAIEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQLQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQKLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRGPPPQETSQ

Target ORF information:

RefSeq Version	XM_006521844.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521844.2

ORF Insert Sequence:

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGAGG GCCCCCCCCA 
CAGGAAACTT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh11 ( XM_006521844.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521907.1 Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X2, mRNA.

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CloneID	OMu51915
Clone ID Related Accession (Same CDS sequence)	XM_006521844.2 , XM_006521844.1
Accession Version	XM_006521844.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	myosin-11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGAGG GCCCCCCCCA 
CAGGAAACTT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521907.1
CDS	145..5982
Translation	MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSITQGPSFAYGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFAIEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQLQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQKLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRGPPPQETSQ

Target ORF information:

RefSeq Version	XM_006521844.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 11, smooth muscle (Myh11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521844.1

ORF Insert Sequence:

ATGGCGCAGA AAGGGCAGCT CAGCGATGAT GAGAAGTTCC TCTTTGTGGA TAAAAACTTC 
ATGAACAGCC CAATGGCTCA GGCCGACTGG GTAGCCAAGA AGCTGGTGTG GGTCCCTTCA 
GAGAAGCAGG GCTTCGAAGC AGCCAGCATC AAGGAGGAGA AGGGCGATGA GGTGGTCGTG 
GAGTTGGTGG AAAATGGAAA GAAGGTCACA GTTGGCAAAG ATGACATCCA AAAAATGAAC 
CCACCCAAGT TCTCTAAGGT GGAGGACATG GCAGAGCTGA CGTGCCTCAA TGAGGCCTCT 
GTGCTGCACA ACCTGAGGGA GCGATACTTC TCAGGCCTCA TCTATACCTA CTCTGGCCTC 
TTCTGTGTGG TGGTCAACCC CTACAAGTAC CTACCCATCT ACTCAGAAAA GATCGTGGAT 
ATGTACAAGG GCAAGAAGAG GCATGAGATG CCGCCTCACA TCTATGCCAT TGCCGACACA 
GCCTACAGAA GCATGCTACA AGATCGTGAA GACCAGTCCA TTCTGTGCAC AGGTGAGTCT 
GGAGCCGGAA AGACAGAGAA CACAAAGAAA GTCATACAGT ACTTGGCTGT GGTGGCATCC 
TCCCACAAGG GCAAGAAAGA CAGCAGCATC ACGCAAGGTC CATCTTTTGC CTACGGGGAG 
CTGGAAAAGC AGCTTCTACA GGCAAACCCA ATCCTGGAGG CTTTCGGCAA TGCGAAAACC 
GTCAAGAACG ACAACTCCTC TCGCTTTGGC AAGTTCATTC GCATCAACTT CGATGTCACT 
GGTTACATTG TAGGTGCCAA TATTGAAACA TATCTTCTGG AAAAGTCTAG GGCTATTCGA 
CAGGCTAGGG ATGAGAGAAC ATTTCACATC TTCTACTACC TGCTCGCCGG AGCCAAGGAA 
AAGATGAAAA GTGACCTGCT TTTGGAGAGC TTCAACAGCT ACACATTTTT ATCCAATGGC 
TTTGTGCCCA TCCCAGCTGC ACAAGATGAT GAGATGTTCC AGGAGACACT GGAAGCCATG 
TCTATCATGG GCTTCAATGA AGAGGAACAG CTAGCCATCT TGAAGGTAGT ATCATCTGTC 
CTTCAGCTTG GAAACATTGT CTTCAAGAAG GAGCGAAACA CAGACCAGGC ATCCATGCCT 
GATAACACAG CGGCTCAGAA AGTTTGCCAC CTCGTGGGGA TTAATGTGAC AGATTTCACT 
AGAGCCATCC TGACCCCACG TATCAAAGTT GGACGGGATG TGGTGCAGAA GGCTCAGACC 
AAAGAACAGG CTGACTTTGC CATCGAGGCC TTAGCCAAGG CCACCTATGA GCGCCTTTTC 
CGATGGATTC TCAGCCGTGT AAACAAGGCC TTGGACAAGA CCCATCGGCA GGGGGCCTCC 
TTCCTGGGCA TTCTGGATAT TGCTGGGTTT GAAATCTTTG AGGTAAACTC CTTCGAGCAG 
CTGTGCATCA ACTACACCAA CGAGAAGCTG CAGCAGCTGT TCAACCACAC GATGTTCATC 
CTGGAGCAGG AAGAGTACCA GCGAGAGGGC ATCGAGTGGA ACTTCATCGA CTTCGGCCTG 
GACCTGCAGC CTTGTATTGA GCTGATTGAG CGGCCGAACA ACCCTCCCGG TGTGCTGGCC 
CTGCTGGATG AAGAATGCTG GTTCCCCAAA GCTACAGACA AGTCTTTTGT GGAGAAGCTA 
TGCTCAGAGC AGGGAAATCA CCCCAAATTT CAGAAGCCCA AGCAGCTAAA GGACAAAACA 
GAGTTCTCCA TCATCCACTA TGCTGGGAAG GTGGACTACA ATGCAAGTGC CTGGCTGACC 
AAGAACATGG ACCCGCTAAA TGACAATGTG ACATCACTCC TCAATGCCTC CTCTGACAAG 
TTTGTGGCTG ACCTGTGGAA GGATGTGGAC CGCATTGTGG GGCTGGACCA GATGGCCAAG 
ATGACTGAGA GCTCACTGCC CAGTGCCTCC AAGACCAAAA AGGGCATGTT CCGCACCGTG 
GGCCAGCTCT ACAAAGAGCA GTTGGGGAAA CTGATGACTA CACTGCGCAA TACCACGCCT 
AACTTTGTGC GCTGCATCAT CCCCAACCAT GAGAAGAGGT CTGGCAAGCT GGATGCATTT 
CTAGTGCTGG AACAGCTGCG CTGCAACGGT GTGTTGGAAG GCATCCGCAT CTGCCGTCAG 
GGCTTCCCCA ACAGGATTGT CTTCCAAGAG TTCCGGCAAC GCTATGAGAT CCTGGCAGCG 
AACGCCATCC CCAAAGGCTT CATGGATGGA AAGCAAGCCT GCATTCTCAT GATCAAAGCC 
CTCGAACTTG ACCCTAACCT GTACAGGATT GGGCAGAGCA AAATCTTCTT CCGAACGGGG 
GTCCTGGCCC ACCTAGAGGA GGAACGAGAC CTGAAAATTA CTGATGTCAT CATGGCCTTC 
CAGGCAATGT GTCGTGGCTA CCTTGCCAGA AAGGCCTTCA CCAAGAGGCA GCAACAGCTG 
ACAGCCATGA AGGTGATCCA GAGGAACTGC GCTGCCTACC TTAAGCTCCG CAACTGGCAA 
TGGTGGCGGC TCTTCACCAA AGTAAAGCCA TTGCTCCAGG TGACACGGCA GGAGGAGGAG 
ATGCAGGCCA AGGAGGAGGA GATGCAAAAG ATCAAGGAGC GGCAGCAGAA GGCAGAGACT 
GAGTTGAAGG AGCTGGAGCA GAAGCACACT CAGCTGGCTG AGGAGAAGAC TCTGCTGCAG 
GAGCAGTTGC AGGCAGAGAC AGAGCTGTAT GCTGAGGCTG AGGAGATGCG GGTCCGGTTG 
GCAGCCAAGA AGCAGGAACT GGAGGAGATC CTACATGAGA TGGAGGCCCG CCTGGAGGAA 
GAGGAAGACC GGGGCCAGCA ACTACAGGCT GAGAGGAAGA AGATGGCTCA GCAGATGCTA 
GACCTGGAGG AGCAACTGGA GGAGGAAGAG GCCGCCAGAC AGAAACTACA GCTAGAGAAG 
GTCACGGCTG AGGCCAAGAT CAAGAAACTG GAGGATGACA TCTTGGTTAT GGATGATCAG 
AACAGTAAAC TTTCAAAAGA ACGAAAACTC CTTGAAGAGA GGGTCAGCGA CTTGACAACC 
AACCTAGCAG AAGAGGAAGA AAAGGCTAAA AACCTCACAA AGCTGAAGAG CAAGCATGAG 
TCTATGATCT CAGAGCTGGA GGTGAGGCTG AAGAAAGAGG AGAAGAGCCG GCAGGAGCTG 
GAGAAACTCA AGAGGAAACT GGAGGGTGAT GCCAGTGACT TCCATGAGCA GATCGCTGAC 
TTGCAGGCCC AGATTGCAGA GCTCAAGATG CAGCTGGCAA AGAAAGAGGA AGAGCTACAG 
GCAGCTCTAG CCAGGCTTGA TGAAGAGATC GCCCAGAAAA ACAATGCCCT AAAGAAGATT 
CGCGAGCTAG AGGGCCATAT CTCAGACCTA CAAGAGGACC TAGACTCAGA GCGGGCTGCC 
AGGAACAAGG CCGAGAAGCA GAAGCGAGAC CTGGGGGAGG AGCTGGAGGC ACTCAAGACG 
GAGCTGGAGG ATACGCTGGA CAGCACAGCT ACCCAGCAGG AGCTCAGAGC CAAGAGGGAA 
CAGGAGGTGA CAGTGCTGAA GAAGGCCCTG GATGAGGAGA CGCGGTCCCA TGAGGCCCAG 
GTCCAGGAGA TGAGGCAGAA GCACACACAG GCAGTGGAGG AACTCACAGA GCAGCTGGAG 
CAGTTCAAAA GGGCCAAGGC AAACCTGGAC AAAAGCAAGC AGACACTGGA GAAGGAGAAC 
GCGGACCTGG CTGGGGAGCT GCGTGTCCTG GGCCAGGCGA AGCAGGAGGT GGAACACAAG 
AAGAAGAAGC TGGAGGTGCA GCTGCAGGAT CTGCAGTCCA AGTGCAGTGA TGGGGAGCGT 
GCCCGGGCTG AGCTCAGTGA CAAGGTCCAC AAGCTACAGA ATGAAGTGGA GAGTGTCACT 
GGCATGCTCA ATGAGGCAGA GGGCAAAGCC ATCAAACTGG CCAAAGATGT GGCTTCCCTT 
GGATCCCAGC TTCAGGACAC CCAAGAGCTG CTCCAAGAAG AAACCCGGCA GAAGCTCAAT 
GTGTCTACCA AGCTGCGTCA GTTGGAAGAT GAAAGGAACA GCCTGCAGGA CCAGCTGGAT 
GAGGAGATGG AGGCTAAGCA AAACCTGGAG CGCCATGTCT CAACACTGAA CATTCAGCTC 
TCAGACTCTA AGAAGAAGCT GCAGGACTTT GCAAGTACCA TCGAGGTCAT GGAGGAGGGG 
AAGAAGAGGT TACAGAAAGA GATGGAGGGC CTCAGCCAGC AGTATGAGGA GAAGGCGGCT 
GCCTATGACA AACTGGAGAA AACCAAGAAC AGGCTCCAGC AGGAGCTGGA TGACCTGGTC 
GTGGACTTGG ACAACCAGCG GCAACTGGTA TCCAATCTGG AAAAGAAGCA GAAGAAATTT 
GACCAGTTGT TAGCTGAGGA GAAGAACATC TCCTCCAAGT ATGCGGATGA GAGAGACCGA 
GCTGAAGCAG AGGCCAGGGA AAAGGAGACA AAGGCTTTGT CTCTAGCCCG GGCCCTGGAG 
GAAGCCCTGG AAGCCAAAGA AGAGCTGGAG AGGACCAACA AGATGCTCAA AGCTGAGATG 
GAAGACCTGG TCAGCTCCAA GGATGATGTA GGCAAGAACG TGCATGAACT GGAGAAGTCC 
AAGCGTGCCT TGGAGACCCA GATGGAAGAG ATGAAAACCC AGCTGGAGGA GTTGGAGGAT 
GAGCTGCAGG CCACTGAGGA TGCCAAGCTG CGGCTAGAGG TCAACATGCA GGCCCTCAAG 
GGCCAGTTTG AACGCGATCT CCAGGCTCGG GATGAACAGA ATGAGGAGAA GAGGAGGCAG 
CTACAGCGGC AGCTGCACGA GTATGAGACA GAACTGGAAG ATGAACGGAA GCAGAGAGCT 
CTGGCGGCGG CAGCTAAGAA GAAGCTGGAA GGGGACCTAA AAGACCTAGA GCTCCAGGCT 
GACTCAGCCA TCAAAGGGAG GGAGGAAGCC ATCAAGCAGC TTCGAAAACT GCAGGCTCAG 
ATGAAGGACT TCCAAAGAGA GCTGGATGAT GCCCGTGCCT CCAGGGATGA GATCTTTGCC 
ACCTCAAAAG AGAATGAGAA GAAAGCCAAG AGTCTGGAGG CAGACCTCAT GCAGCTCCAA 
GAGGACCTGG CAGCAGCTGA GAGAGCTCGC AAGCAAGCTG ACCTGGAGAA GGAGGAGCTG 
GCCGAGGAGC TGGCTAGCAG CTTGTCAGGA AGGAATACAC TGCAGGATGA GAAGCGCCGC 
CTGGAGGCAA GGATCGCCCA ACTAGAGGAG GAGCTGGAGG AAGAGCAGGG CAACATGGAG 
GCCATGAGTG ATAGAGTACG CAAGGCCACA CTGCAGGCTG AGCAACTGAG CAATGAGCTG 
GCCACAGAAC GCAGCACGGC TCAGAAGAAT GAGAGCGCAC GGCAACAGCT GGAGCGCCAG 
AACAAGGAAC TGCGAAGCAA GTTGCAGGAG GTAGAAGGTG CTGTCAAAGC CAAGCTCAAG 
TCCACTGTTG CGGCGCTGGA GGCCAAGATT GCACAGCTGG AGGAGCAGGT TGAACAGGAG 
GCCAGAGAGA AACAGGCGGC CACCAAGTCG CTGAAGCAAA AGGACAAGAA GCTAAAGGAG 
GTCCTGCTGC AGGTGGAGGA TGAGCGCAAG ATGGCAGAGC AGTACAAGGA GCAGGCAGAG 
AAAGGAAACA CCAAGGTCAA GCAGCTGAAG AGGCAGCTGG AAGAGGCAGA GGAGGAGTCC 
CAGCGCATCA ACGCCAACCG CAGGAAGCTG CAGCGGGAGC TAGATGAGGC CACAGAGAGC 
AATGAGGCCA TGGGCCGTGA GGTGAACGCC CTCAAGAGCA AACTCAGAGG GCCCCCCCCA 
CAGGAAACTT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Delayed onset of smooth muscle cell differentiation leads to hydroureter formation in mice with conditional loss of the zinc finger transcription factor gene Gata2 in the ureteric mesenchyme.
The Journal of pathology248(4)452-463(2019 Aug)
Weiss AC,Bohnenpoll T,Kurz J,Blank P,Airik R,Lüdtke TH,Kleppa MJ,Deuper L,Kaiser M,Mamo TM,Costa R,von Hahn T,Trowe MO,Kispert A

HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis.
Development (Cambridge, England)146(13)(2019 Jul)
Xia M,Luo W,Jin H,Yang Z

Tie2 regulates endocardial sprouting and myocardial trabeculation.
JCI insight5(2019 May)
Qu X,Harmelink C,Baldwin HS

FOXF1 transcription factor promotes lung morphogenesis by inducing cellular proliferation in fetal lung mesenchyme.
Developmental biology443(1)50-63(2018 11)
Ustiyan V,Bolte C,Zhang Y,Han L,Xu Y,Yutzey KE,Zorn AM,Kalin TV,Shannon JM,Kalinichenko VV

TBX2 and TBX3 act downstream of canonical WNT signaling in patterning and differentiation of the mouse ureteric mesenchyme.
Development (Cambridge, England)145(23)(2018 11)
Aydoğdu N,Rudat C,Trowe MO,Kaiser M,Lüdtke TH,Taketo MM,Christoffels VM,Moon A,Kispert A

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Myh11 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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