NHSL1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	NHSL1
Entrez Gene ID	100618251
Full Name	NHS like 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007484627.2	XP_007484689.1	NHS-like protein 1 isoform X4
XM_007484628.2	XP_007484690.1	NHS-like protein 1 isoform X5
XM_007484624.2	XP_007484686.1	NHS-like protein 1 isoform X1
XM_007484625.2	XP_007484687.1	NHS-like protein 1 isoform X2
XM_007484626.2	XP_007484688.1	NHS-like protein 1 isoform X3
XM_007484629.2	XP_007484691.1	NHS-like protein 1 isoform X6

Pathways from BioSystems
Gene Ontology

The following NHSL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHSL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc12983	XM_007484624.2 Latest version!	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc12984	XM_007484625.2 Latest version!	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc12985	XM_007484626.2 Latest version!	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc12986	XM_007484627.2 Latest version!	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc12987	XM_007484628.2 Latest version!	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc12988	XM_007484629.2 Latest version!	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NHSL1 ( XM_007484624.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484686.1 Monodelphis domestica NHS like 1 (NHSL1), transcript variant X1, mRNA.

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CloneID	OMc12983
Clone ID Related Accession (Same CDS sequence)	XM_007484624.2
Accession Version	XM_007484624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007484624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTC ACAAGAGGAC TGTGGAGCCC GTAAAGATGT GCCGGCTGGA CGAAGCTGCC 
GGAGACGAGG CTGCCGGGGT CTCCGGGCGA TGCGGAAGCG GTCCCGGGTC AGGGAAGCTG 
GGGCGGGCGC CGCAGCCGTC GGCGGGCGCT GCGGGAGGAG CCGCTACGGC CGAAGGAGCC 
GGGGCTGGGG CCGGGGGGCT GCTCTTCACT TCCCTGGAGC AGGTCAGCGC GCACTCTCTG 
ATTTTCTTGC TCTGGCAGCT CTCTGACCTC TCCCGTTGCG CGGGGGACAT CTTCGCCGAG 
ATAGAGGGGC AGGCAACTGC CCTGGGTCGC CGGGCCGCCC GGCTCCACGG CCGACTGGGC 
GCTCTGCAAG CCGCGGCCAG CAGACTGGAC CACCGCAAAG TCAAAATCCC TGTCTCCAAT 
TTAGATGAAG AGAGTAGATG GACAGTTCAT TACACAGCAC CATGGCATCA GCAGGAGAAT 
GTGTTCCTAC CCACCACAAG GCCACCCTGT GTGGAAGACT TGCATCGACA AGCCAAGCTG 
AACCTCAAAT CAGTACTGAG AGAATGTGAC AAACTGAGGA GAGATGGTTA TCGAAGCTCT 
CAGTACTACT CTCAGGGACC TACTTTCTCT AAATCCAGCT TGGCCTGTGA CACCTACAAA 
GATGAAGATG AAGAAAGTGA TCAGAAGTGT TCCATCTCTT CCTCAGAGGA AGAAAAACTC 
ATTGCTACCA GGAGAGCCAG AACTCCCACT TCAAATGATT TATCTGATAT CAACACGCAG 
ACAAATTGGA CCAAGTCACT TCCCCTGCCA ACACCAGAGG AAAAGATGAG ACAGCAAGCA 
CAAACAGTCC AGACAGATGT GGTTCCTATT AATGTAACTG GGGAGAATTT CGACCGCCAA 
GCCAGCCTTC GGCGGTCTCT AATTTACACA GACACTGTCG TAAGACGGCC GAAGAAAGTC 
AAAAGGAGAA AGACTATTAC AGGAATCCCT GACATCATAC AAAAGGAGCT AGCTACTGGC 
ACTAGCCAGA ATGACTTCCG TGGTCACTCG ATGTATGTCC CCGACCACTA CTCCACACTA 
GGAAGGCTTG ACAACTATCG ACCCGCCATT CAGCGTTCTG AAACCAGGGA CTCTAGTTGT 
CAGACTGATG AAGTAAAAGT TGTGCCCCCT TCCATGAGAA GAATCAGGGC ACAAAAGGGG 
CAAGGTATTG CTGCCCAGAT GAGCCAGTTC TCCGTCTCAT CAGGGAACAT GTCAGTGATG 
AGCGATTCTG CAGGTGTTGT GTTTCCTCCA CGCTTCCACA GTGATGTGGG TTTCCATAGC 
CTTCCTCGTC CTGGAACAAG GTTGTCCCTT CAACCTCTCG AGTCACCCCA GGCTGTTGAG 
AACCCTGCAG AGGACTTGGC TGGCATTCTC GCCTACCAGA TGGGTAAGCT GCAAGTTGAT 
GAAAACTTTG AGCATGCGAG ATGTATTTCA ACACCTGGGA CATTATCGAG ACCTAAATCA 
GAAGAGTTGA GACATTATGA AAGTGAAAAA ACAAGCCCAG CCTGTATTGT TTCTCCTCAT 
GCTGCCTACT CTACCAGCCT CATAGCAAAT GCCACAATTT CTTCATCATC GGAGGTTATT 
GCTATACACA CAGTCCAGAA TACTGGACAG CTGGACAGCA GAATCGCCAG CTCATCCCCT 
TATGCCAGGA TGATGAAGTC CAGAGACAAT CTTGCCACCA GCAGTGCCAT AAAAGATGAT 
CATCGGTCTT CCAGTGGTAA CTGGAGCGAG AGTAGCTCGA CACGACACTC ACAGACTTCA 
GACACCATTA CCAGCAATGT AGTCATGATG GCGTCCCTCT GTGACTCTGA TGCCTCTCTG 
AACACTCCAG GAAACAAGGA GAATGGGTCC CGAAATGTAG CCTACAACAG CAGAAACAAC 
CTGAATTTCC TAAGCCATTC CCAAGACAGC GATGGTAAGA GTGAGTCCAG TTACTCAGGT 
GACAGAGCAC CTGGGGGCAC AGAGCCCTGG GAATATAAAG CATCAGGCAG TGAGAGGGCA 
TCCCCACACA AGTCACATTG TATTACTCCT GGCTACTCCA CTCCTGCTAG TAACATGAGC 
AGCAGTAGTT TAGACCAAGC CTCAGTCAAA GAGGATGCAA ATTCTCTCTA CTCAGTAGAC 
CATGATGGCT ACTACACTTC CATGCACATG GACTCTGGAA TCAAATCAAG AAATCGGTGC 
AATAGCAGTG ATGGCTTTGG GAACCCCAGG CACAGTGTGA TTAATGTTTT TGATGGTAAA 
GCCCAGAAAA ACCAAGGTGA CCAGTTGCAA AACTGTGATA AATCTCTGCC ACGAAACATC 
TCCTTAAAGA AAGCGAAGAA ACCTCCCCTT CCCCCTTTCA GAACAGATTC CCTTCGAAGG 
TCCCCCAAGA AAAAAACTCA CCCCAATGGG CAGGTGCTAA ATGAGACTCT GATTGCTACC 
CTCCAACATT CCCTACAACT GAATCTGCAG CGGAAAGATA GTAGTTCTCC TTCCCAGAGC 
CCCTGTAGCG ATTACGAAGA TCCATGGCTC CTCCGTTCCC GTAGCCAGAG CACAGTCAGC 
ACGAGCAGCA GCATGGTGTC GGCCACAGCA CCAAATGTGT ATTCACTTTG CGCGGTCACC 
CCATCTCAGA GCGACACAAG CAGCATCAAG TCAGAGTACG GAGATCCTTG GGGTTACTAC 
ATCGACTATA CTGGCGTACA AGAAGATCAG GGGAAATCCA GTGGCACAGG TCCACCTAAT 
GCTGTGACTC CATTGTGTGA TCTTGCCACC AGCCATCTCC AGGATGGATG CAGGGTGCCT 
GTCCCACAAG TGTCTAGTGG ATTGGCCAAA CCAAAGAACG CTTCCCCAGA GAAGTCTCAC 
AGGGTCACTT CCCCATCTAG TGGCTATTCC AGCCAATCGA ATACCCCCAC AGCGCTTACT 
CCAGTGCCTG TGTTTTTAAA ATCTATCTCA CCAGGAAATG GGAAATCAAA GTTGAAGCCC 
AAAGTGCCTG AAAGAAAATC CTCCCTGATA TCTTCAGTAT CTATCTCTTC ATCTTCAACT 
TCCCTTTCTT CCAATACTTC AACTGAAGGC AGTGTGAATA TGAAGAAATT AGATTCAGCT 
GTAAGCACTC CTCCCATTCC TCCTCTTTCC CCAGCATCTG TACATCCTCT TAATCACTCC 
CCTCTCCTTC CTCCTCCTCC TTTAGCAGAT GCCTTGGAAC TGTCCACTGT ACAGCATTCT 
CCCATTTTTC CTCCTCCACC ACCAGAAGTC CTTGCTTCTT CTTTTCTTCC AACGGATACA 
TGCTTTCCTC TTCCCCCAGC ATCCTTCGGT ATGCCCAATT CAACTGCTCC CTTGCCACCT 
CCTCCTTCAT CTCTCTTTCC CTCTTCTGTG CCTCCACCTG CTCCACCTCT TGACCCTAAG 
TTGATGAAAG AAGCTTGGCC CTCTTTAAAA AAGTGTGGCC AACAGGATTC TTCTTGTGCT 
GTACTTAAAC CGTCTCTCAC CAAGGAAGAA ACCACTAAAC CACCAATGCC CCTAATAACC 
ACTAAAGCAT TACAGATGGT ACGGTTGAGG CCTGTGAACA AGCCCTCAGA AACATCAGGA 
GCTCAGCCAG GCAAACCAGC TTCTGAAACA GAATCTCAGG AAAATAATTC TGTGGTGCCA 
CAGGATCCTT TAAAACCATC TTCTGCCTTG AAACACAGAG GGAGCATCAA TGGACTAGAC 
AGCAGCAAAG CCCAAAATGT CCCCGTTAAA AACACACCTC CAACTCCTGA GAAGGGCTCG 
TTTTGGGGTT GGCACAGCAG GACAGCTGAG CTTGCTGCTC TTCAAAGGCA TGGGGATGCA 
GCTGATCCTG CACCATCCTC CGAAGCATCC TTTTCAGGAA CAAATGCTGA GGAAGGAGGG 
CAGGTTTCTG GGCAGGACGA GGAATCTCCT TCTGGCCTCA GGACTGCATT GCTCCAGGAG 
CAGCCCAGCT CTCCAAAGAA GACACATGGC TCTTCTTCAC CCAGCAGGAA ACCACCTCCT 
ATTTCAAAGA AACCCAAACT GTTCCTTGTC GTGCCACCTC CGCACATAGA TTTCACAGCA 
GAGAAAACAA CCAAAGTGAA TGAAAGTGTC AGCAGCCCAG CCAGGGGAGA AGTGGTTGAT 
GTGAATGGCA AAGAGGCTCA AGAGAGCTTT GCTGCAGGAC CTGGTTCTGC CGAGACAGAC 
TTTGGCAGCT TGGTGCCAGG GGGTGGAGCT GTGGGATCCA CCTTCCTTGC AGGAGTGGGA 
GCCGATGTCT ATATGGTACA GCCTGATGTA TCACCAGCTC AAGGAGCTCC CAAGCAGGAG 
GAGCCAACCA CTTCAGATGA AGGCAGCAGG ATAGAGAGCA CTGAAGACAG TGGTGGTAAC 
GTGGGCAGTC CCCCCTCTCG AGAGGATGGT AACATTGGGG TATTTGAGGC TGACATGGTA 
TGTACCTCTC CTGGACCATG CAGTAGCCTT AAGGAAGATG GGGGTGATGA GGTGACAACT 
CCAAGTAGAC CCAGAACTAC AGAAGACCTT TTTGCAGCTA TCCACAGGTC TAAGAGGAAA 
GTCCTTGGCC GAAAGGATTC TGATGATGAT CATTCCCGAA ACCATTCTCC ATCACCTCCA 
GTGACACCCA CTGGGGCTGC CCCCAGCCTG GCTTCCCTTA AGCAAGCAGG ATCCATCCAG 
AGAAGCATTC GTAAGAGTAG TACTAGCAGT GATAACTTCA AAGCTCTGCT GCTGAAAAAG 
GGGAGCCGGA CAGATGCCAG CTCTCGAATG TCAGCGGCTG AGATGCTGAA GAATACAGAC 
CCAAGGTTTC ACAGATCCAA GTCGGAGTCC TCGTCAGACA CCCCTGACAG CCCCTCAAGC 
TGTTCTCCAA ACAAGAACAG GAGGGCCCAG GAGGAGTGGG CCAAGAATGA AGGCCTGATG 
CCAAGGAGTC TGTCCTTCTC TGGCACCAGG TATGGCCGGA GCCGGACACC GCCTTCTGCT 
GCAAGTAGCA GGTACAGCAT GAGGAACCGA ATTCAAAGCA GCCCCATGAC TGTCATTTCT 
GAGGGAGAAG GGGAAGCAAC AGAGCCCACA GAGAACAGAC TCCATCGGAC TGCAGGAGTT 
GGAAGGGAAA AAGAAATGGA TGGATTTACT GGGAATGAAA TGGACACTAG TGATCTGCCA 
TATTCTGAAG AGTGTGGCTC CAAGGGAGCT CAGGCTCCTA ACCATGTTGA CTCCACAGAT 
AAGACAGCTA GTAAGACTCT ATCAGGACAA TGTAGAAGTT CTTTAAGGGA AGAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484686.1
CDS	187..5466
Translation	MPFHKRTVEPVKMCRLDEAAGDEAAGVSGRCGSGPGSGKLGRAPQPSAGAAGGAATAEGAGAGAGGLLFTSLEQVSAHSLIFLLWQLSDLSRCAGDIFAEIEGQATALGRRAARLHGRLGALQAAASRLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSKSSLACDTYKDEDEESDQKCSISSSEEEKLIATRRARTPTSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQTVQTDVVPINVTGENFDRQASLRRSLIYTDTVVRRPKKVKRRKTITGIPDIIQKELATGTSQNDFRGHSMYVPDHYSTLGRLDNYRPAIQRSETRDSSCQTDEVKVVPPSMRRIRAQKGQGIAAQMSQFSVSSGNMSVMSDSAGVVFPPRFHSDVGFHSLPRPGTRLSLQPLESPQAVENPAEDLAGILAYQMGKLQVDENFEHARCISTPGTLSRPKSEELRHYESEKTSPACIVSPHAAYSTSLIANATISSSSEVIAIHTVQNTGQLDSRIASSSPYARMMKSRDNLATSSAIKDDHRSSSGNWSESSSTRHSQTSDTITSNVVMMASLCDSDASLNTPGNKENGSRNVAYNSRNNLNFLSHSQDSDGKSESSYSGDRAPGGTEPWEYKASGSERASPHKSHCITPGYSTPASNMSSSSLDQASVKEDANSLYSVDHDGYYTSMHMDSGIKSRNRCNSSDGFGNPRHSVINVFDGKAQKNQGDQLQNCDKSLPRNISLKKAKKPPLPPFRTDSLRRSPKKKTHPNGQVLNETLIATLQHSLQLNLQRKDSSSPSQSPCSDYEDPWLLRSRSQSTVSTSSSMVSATAPNVYSLCAVTPSQSDTSSIKSEYGDPWGYYIDYTGVQEDQGKSSGTGPPNAVTPLCDLATSHLQDGCRVPVPQVSSGLAKPKNASPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSISPGNGKSKLKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSVNMKKLDSAVSTPPIPPLSPASVHPLNHSPLLPPPPLADALELSTVQHSPIFPPPPPEVLASSFLPTDTCFPLPPASFGMPNSTAPLPPPPSSLFPSSVPPPAPPLDPKLMKEAWPSLKKCGQQDSSCAVLKPSLTKEETTKPPMPLITTKALQMVRLRPVNKPSETSGAQPGKPASETESQENNSVVPQDPLKPSSALKHRGSINGLDSSKAQNVPVKNTPPTPEKGSFWGWHSRTAELAALQRHGDAADPAPSSEASFSGTNAEEGGQVSGQDEESPSGLRTALLQEQPSSPKKTHGSSSPSRKPPPISKKPKLFLVVPPPHIDFTAEKTTKVNESVSSPARGEVVDVNGKEAQESFAAGPGSAETDFGSLVPGGGAVGSTFLAGVGADVYMVQPDVSPAQGAPKQEEPTTSDEGSRIESTEDSGGNVGSPPSREDGNIGVFEADMVCTSPGPCSSLKEDGGDEVTTPSRPRTTEDLFAAIHRSKRKVLGRKDSDDDHSRNHSPSPPVTPTGAAPSLASLKQAGSIQRSIRKSSTSSDNFKALLLKKGSRTDASSRMSAAEMLKNTDPRFHRSKSESSSDTPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGTRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEATEPTENRLHRTAGVGREKEMDGFTGNEMDTSDLPYSEECGSKGAQAPNHVDSTDKTASKTLSGQCRSSLREES

Target ORF information:

RefSeq Version	XM_007484624.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007484624.2

ORF Insert Sequence:

ATGCCTTTTC ACAAGAGGAC TGTGGAGCCC GTAAAGATGT GCCGGCTGGA CGAAGCTGCC 
GGAGACGAGG CTGCCGGGGT CTCCGGGCGA TGCGGAAGCG GTCCCGGGTC AGGGAAGCTG 
GGGCGGGCGC CGCAGCCGTC GGCGGGCGCT GCGGGAGGAG CCGCTACGGC CGAAGGAGCC 
GGGGCTGGGG CCGGGGGGCT GCTCTTCACT TCCCTGGAGC AGGTCAGCGC GCACTCTCTG 
ATTTTCTTGC TCTGGCAGCT CTCTGACCTC TCCCGTTGCG CGGGGGACAT CTTCGCCGAG 
ATAGAGGGGC AGGCAACTGC CCTGGGTCGC CGGGCCGCCC GGCTCCACGG CCGACTGGGC 
GCTCTGCAAG CCGCGGCCAG CAGACTGGAC CACCGCAAAG TCAAAATCCC TGTCTCCAAT 
TTAGATGAAG AGAGTAGATG GACAGTTCAT TACACAGCAC CATGGCATCA GCAGGAGAAT 
GTGTTCCTAC CCACCACAAG GCCACCCTGT GTGGAAGACT TGCATCGACA AGCCAAGCTG 
AACCTCAAAT CAGTACTGAG AGAATGTGAC AAACTGAGGA GAGATGGTTA TCGAAGCTCT 
CAGTACTACT CTCAGGGACC TACTTTCTCT AAATCCAGCT TGGCCTGTGA CACCTACAAA 
GATGAAGATG AAGAAAGTGA TCAGAAGTGT TCCATCTCTT CCTCAGAGGA AGAAAAACTC 
ATTGCTACCA GGAGAGCCAG AACTCCCACT TCAAATGATT TATCTGATAT CAACACGCAG 
ACAAATTGGA CCAAGTCACT TCCCCTGCCA ACACCAGAGG AAAAGATGAG ACAGCAAGCA 
CAAACAGTCC AGACAGATGT GGTTCCTATT AATGTAACTG GGGAGAATTT CGACCGCCAA 
GCCAGCCTTC GGCGGTCTCT AATTTACACA GACACTGTCG TAAGACGGCC GAAGAAAGTC 
AAAAGGAGAA AGACTATTAC AGGAATCCCT GACATCATAC AAAAGGAGCT AGCTACTGGC 
ACTAGCCAGA ATGACTTCCG TGGTCACTCG ATGTATGTCC CCGACCACTA CTCCACACTA 
GGAAGGCTTG ACAACTATCG ACCCGCCATT CAGCGTTCTG AAACCAGGGA CTCTAGTTGT 
CAGACTGATG AAGTAAAAGT TGTGCCCCCT TCCATGAGAA GAATCAGGGC ACAAAAGGGG 
CAAGGTATTG CTGCCCAGAT GAGCCAGTTC TCCGTCTCAT CAGGGAACAT GTCAGTGATG 
AGCGATTCTG CAGGTGTTGT GTTTCCTCCA CGCTTCCACA GTGATGTGGG TTTCCATAGC 
CTTCCTCGTC CTGGAACAAG GTTGTCCCTT CAACCTCTCG AGTCACCCCA GGCTGTTGAG 
AACCCTGCAG AGGACTTGGC TGGCATTCTC GCCTACCAGA TGGGTAAGCT GCAAGTTGAT 
GAAAACTTTG AGCATGCGAG ATGTATTTCA ACACCTGGGA CATTATCGAG ACCTAAATCA 
GAAGAGTTGA GACATTATGA AAGTGAAAAA ACAAGCCCAG CCTGTATTGT TTCTCCTCAT 
GCTGCCTACT CTACCAGCCT CATAGCAAAT GCCACAATTT CTTCATCATC GGAGGTTATT 
GCTATACACA CAGTCCAGAA TACTGGACAG CTGGACAGCA GAATCGCCAG CTCATCCCCT 
TATGCCAGGA TGATGAAGTC CAGAGACAAT CTTGCCACCA GCAGTGCCAT AAAAGATGAT 
CATCGGTCTT CCAGTGGTAA CTGGAGCGAG AGTAGCTCGA CACGACACTC ACAGACTTCA 
GACACCATTA CCAGCAATGT AGTCATGATG GCGTCCCTCT GTGACTCTGA TGCCTCTCTG 
AACACTCCAG GAAACAAGGA GAATGGGTCC CGAAATGTAG CCTACAACAG CAGAAACAAC 
CTGAATTTCC TAAGCCATTC CCAAGACAGC GATGGTAAGA GTGAGTCCAG TTACTCAGGT 
GACAGAGCAC CTGGGGGCAC AGAGCCCTGG GAATATAAAG CATCAGGCAG TGAGAGGGCA 
TCCCCACACA AGTCACATTG TATTACTCCT GGCTACTCCA CTCCTGCTAG TAACATGAGC 
AGCAGTAGTT TAGACCAAGC CTCAGTCAAA GAGGATGCAA ATTCTCTCTA CTCAGTAGAC 
CATGATGGCT ACTACACTTC CATGCACATG GACTCTGGAA TCAAATCAAG AAATCGGTGC 
AATAGCAGTG ATGGCTTTGG GAACCCCAGG CACAGTGTGA TTAATGTTTT TGATGGTAAA 
GCCCAGAAAA ACCAAGGTGA CCAGTTGCAA AACTGTGATA AATCTCTGCC ACGAAACATC 
TCCTTAAAGA AAGCGAAGAA ACCTCCCCTT CCCCCTTTCA GAACAGATTC CCTTCGAAGG 
TCCCCCAAGA AAAAAACTCA CCCCAATGGG CAGGTGCTAA ATGAGACTCT GATTGCTACC 
CTCCAACATT CCCTACAACT GAATCTGCAG CGGAAAGATA GTAGTTCTCC TTCCCAGAGC 
CCCTGTAGCG ATTACGAAGA TCCATGGCTC CTCCGTTCCC GTAGCCAGAG CACAGTCAGC 
ACGAGCAGCA GCATGGTGTC GGCCACAGCA CCAAATGTGT ATTCACTTTG CGCGGTCACC 
CCATCTCAGA GCGACACAAG CAGCATCAAG TCAGAGTACG GAGATCCTTG GGGTTACTAC 
ATCGACTATA CTGGCGTACA AGAAGATCAG GGGAAATCCA GTGGCACAGG TCCACCTAAT 
GCTGTGACTC CATTGTGTGA TCTTGCCACC AGCCATCTCC AGGATGGATG CAGGGTGCCT 
GTCCCACAAG TGTCTAGTGG ATTGGCCAAA CCAAAGAACG CTTCCCCAGA GAAGTCTCAC 
AGGGTCACTT CCCCATCTAG TGGCTATTCC AGCCAATCGA ATACCCCCAC AGCGCTTACT 
CCAGTGCCTG TGTTTTTAAA ATCTATCTCA CCAGGAAATG GGAAATCAAA GTTGAAGCCC 
AAAGTGCCTG AAAGAAAATC CTCCCTGATA TCTTCAGTAT CTATCTCTTC ATCTTCAACT 
TCCCTTTCTT CCAATACTTC AACTGAAGGC AGTGTGAATA TGAAGAAATT AGATTCAGCT 
GTAAGCACTC CTCCCATTCC TCCTCTTTCC CCAGCATCTG TACATCCTCT TAATCACTCC 
CCTCTCCTTC CTCCTCCTCC TTTAGCAGAT GCCTTGGAAC TGTCCACTGT ACAGCATTCT 
CCCATTTTTC CTCCTCCACC ACCAGAAGTC CTTGCTTCTT CTTTTCTTCC AACGGATACA 
TGCTTTCCTC TTCCCCCAGC ATCCTTCGGT ATGCCCAATT CAACTGCTCC CTTGCCACCT 
CCTCCTTCAT CTCTCTTTCC CTCTTCTGTG CCTCCACCTG CTCCACCTCT TGACCCTAAG 
TTGATGAAAG AAGCTTGGCC CTCTTTAAAA AAGTGTGGCC AACAGGATTC TTCTTGTGCT 
GTACTTAAAC CGTCTCTCAC CAAGGAAGAA ACCACTAAAC CACCAATGCC CCTAATAACC 
ACTAAAGCAT TACAGATGGT ACGGTTGAGG CCTGTGAACA AGCCCTCAGA AACATCAGGA 
GCTCAGCCAG GCAAACCAGC TTCTGAAACA GAATCTCAGG AAAATAATTC TGTGGTGCCA 
CAGGATCCTT TAAAACCATC TTCTGCCTTG AAACACAGAG GGAGCATCAA TGGACTAGAC 
AGCAGCAAAG CCCAAAATGT CCCCGTTAAA AACACACCTC CAACTCCTGA GAAGGGCTCG 
TTTTGGGGTT GGCACAGCAG GACAGCTGAG CTTGCTGCTC TTCAAAGGCA TGGGGATGCA 
GCTGATCCTG CACCATCCTC CGAAGCATCC TTTTCAGGAA CAAATGCTGA GGAAGGAGGG 
CAGGTTTCTG GGCAGGACGA GGAATCTCCT TCTGGCCTCA GGACTGCATT GCTCCAGGAG 
CAGCCCAGCT CTCCAAAGAA GACACATGGC TCTTCTTCAC CCAGCAGGAA ACCACCTCCT 
ATTTCAAAGA AACCCAAACT GTTCCTTGTC GTGCCACCTC CGCACATAGA TTTCACAGCA 
GAGAAAACAA CCAAAGTGAA TGAAAGTGTC AGCAGCCCAG CCAGGGGAGA AGTGGTTGAT 
GTGAATGGCA AAGAGGCTCA AGAGAGCTTT GCTGCAGGAC CTGGTTCTGC CGAGACAGAC 
TTTGGCAGCT TGGTGCCAGG GGGTGGAGCT GTGGGATCCA CCTTCCTTGC AGGAGTGGGA 
GCCGATGTCT ATATGGTACA GCCTGATGTA TCACCAGCTC AAGGAGCTCC CAAGCAGGAG 
GAGCCAACCA CTTCAGATGA AGGCAGCAGG ATAGAGAGCA CTGAAGACAG TGGTGGTAAC 
GTGGGCAGTC CCCCCTCTCG AGAGGATGGT AACATTGGGG TATTTGAGGC TGACATGGTA 
TGTACCTCTC CTGGACCATG CAGTAGCCTT AAGGAAGATG GGGGTGATGA GGTGACAACT 
CCAAGTAGAC CCAGAACTAC AGAAGACCTT TTTGCAGCTA TCCACAGGTC TAAGAGGAAA 
GTCCTTGGCC GAAAGGATTC TGATGATGAT CATTCCCGAA ACCATTCTCC ATCACCTCCA 
GTGACACCCA CTGGGGCTGC CCCCAGCCTG GCTTCCCTTA AGCAAGCAGG ATCCATCCAG 
AGAAGCATTC GTAAGAGTAG TACTAGCAGT GATAACTTCA AAGCTCTGCT GCTGAAAAAG 
GGGAGCCGGA CAGATGCCAG CTCTCGAATG TCAGCGGCTG AGATGCTGAA GAATACAGAC 
CCAAGGTTTC ACAGATCCAA GTCGGAGTCC TCGTCAGACA CCCCTGACAG CCCCTCAAGC 
TGTTCTCCAA ACAAGAACAG GAGGGCCCAG GAGGAGTGGG CCAAGAATGA AGGCCTGATG 
CCAAGGAGTC TGTCCTTCTC TGGCACCAGG TATGGCCGGA GCCGGACACC GCCTTCTGCT 
GCAAGTAGCA GGTACAGCAT GAGGAACCGA ATTCAAAGCA GCCCCATGAC TGTCATTTCT 
GAGGGAGAAG GGGAAGCAAC AGAGCCCACA GAGAACAGAC TCCATCGGAC TGCAGGAGTT 
GGAAGGGAAA AAGAAATGGA TGGATTTACT GGGAATGAAA TGGACACTAG TGATCTGCCA 
TATTCTGAAG AGTGTGGCTC CAAGGGAGCT CAGGCTCCTA ACCATGTTGA CTCCACAGAT 
AAGACAGCTA GTAAGACTCT ATCAGGACAA TGTAGAAGTT CTTTAAGGGA AGAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_007484625.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484687.1 Monodelphis domestica NHS like 1 (NHSL1), transcript variant X2, mRNA.

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CloneID	OMc12984
Clone ID Related Accession (Same CDS sequence)	XM_007484625.2
Accession Version	XM_007484625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007484625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTC ACAAGAGGAC TGTGGAGCCC GTAAAGATGT GCCGGCTGGA CGAAGCTGCC 
GGAGACGAGG CTGCCGGGGT CTCCGGGCGA TGCGGAAGCG GTCCCGGGTC AGGGAAGCTG 
GGGCGGGCGC CGCAGCCGTC GGCGGGCGCT GCGGGAGGAG CCGCTACGGC CGAAGGAGCC 
GGGGCTGGGG CCGGGGGGCT GCTCTTCACT TCCCTGGAGC AGGTCAGCGC GCACTCTCTG 
ATTTTCTTGC TCTGGCAGCT CTCTGACCTC TCCCGTTGCG CGGGGGACAT CTTCGCCGAG 
ATAGAGGGGC AGGCAACTGC CCTGGGTCGC CGGGCCGCCC GGCTCCACGG CCGACTGGGC 
GCTCTGCAAG CCGCGGCCAG CAGACTGGAC CACCGCAAAG TCAAAATCCC TGTCTCCAAT 
TTAGATGAAG AGAGTAGATG GACAGTTCAT TACACAGCAC CATGGCATCA GCAGGAGAAT 
GTGTTCCTAC CCACCACAAG GCCACCCTGT GTGGAAGACT TGCATCGACA AGCCAAGCTG 
AACCTCAAAT CAGTACTGAG AGAATGTGAC AAACTGAGGA GAGATGGTTA TCGAAGCTCT 
CAGTACTACT CTCAGGGACC TACTTTCTCT AAATCCAGCT TGGCCTGTGA CACCTACAAA 
GATGAAGATG AAGAAAGTGA TCAGAAGTGT TCCATCTCTT CCTCAGAGGA AGAAAAACTC 
ATTGCTACCA GGAGAGCCAG AACTCCCACT TCAAATGATT TATCTGATAT CAACACGCAG 
ACAAATTGGA CCAAGTCACT TCCCCTGCCA ACACCAGAGG AAAAGATGAG ACAGCAAGCA 
CAAACAGTCC AGACAGATGT GGTTCCTATT AATGTAACTG CTACTGGCAC TAGCCAGAAT 
GACTTCCGTG GTCACTCGAT GTATGTCCCC GACCACTACT CCACACTAGG AAGGCTTGAC 
AACTATCGAC CCGCCATTCA GCGTTCTGAA ACCAGGGACT CTAGTTGTCA GACTGATGAA 
GTAAAAGTTG TGCCCCCTTC CATGAGAAGA ATCAGGGCAC AAAAGGGGCA AGGTATTGCT 
GCCCAGATGA GCCAGTTCTC CGTCTCATCA GGGAACATGT CAGTGATGAG CGATTCTGCA 
GGTGTTGTGT TTCCTCCACG CTTCCACAGT GATGTGGGTT TCCATAGCCT TCCTCGTCCT 
GGAACAAGGT TGTCCCTTCA ACCTCTCGAG TCACCCCAGG CTGTTGAGAA CCCTGCAGAG 
GACTTGGCTG GCATTCTCGC CTACCAGATG GGTAAGCTGC AAGTTGATGA AAACTTTGAG 
CATGCGAGAT GTATTTCAAC ACCTGGGACA TTATCGAGAC CTAAATCAGA AGAGTTGAGA 
CATTATGAAA GTGAAAAAAC AAGCCCAGCC TGTATTGTTT CTCCTCATGC TGCCTACTCT 
ACCAGCCTCA TAGCAAATGC CACAATTTCT TCATCATCGG AGGTTATTGC TATACACACA 
GTCCAGAATA CTGGACAGCT GGACAGCAGA ATCGCCAGCT CATCCCCTTA TGCCAGGATG 
ATGAAGTCCA GAGACAATCT TGCCACCAGC AGTGCCATAA AAGATGATCA TCGGTCTTCC 
AGTGGTAACT GGAGCGAGAG TAGCTCGACA CGACACTCAC AGACTTCAGA CACCATTACC 
AGCAATGTAG TCATGATGGC GTCCCTCTGT GACTCTGATG CCTCTCTGAA CACTCCAGGA 
AACAAGGAGA ATGGGTCCCG AAATGTAGCC TACAACAGCA GAAACAACCT GAATTTCCTA 
AGCCATTCCC AAGACAGCGA TGGTAAGAGT GAGTCCAGTT ACTCAGGTGA CAGAGCACCT 
GGGGGCACAG AGCCCTGGGA ATATAAAGCA TCAGGCAGTG AGAGGGCATC CCCACACAAG 
TCACATTGTA TTACTCCTGG CTACTCCACT CCTGCTAGTA ACATGAGCAG CAGTAGTTTA 
GACCAAGCCT CAGTCAAAGA GGATGCAAAT TCTCTCTACT CAGTAGACCA TGATGGCTAC 
TACACTTCCA TGCACATGGA CTCTGGAATC AAATCAAGAA ATCGGTGCAA TAGCAGTGAT 
GGCTTTGGGA ACCCCAGGCA CAGTGTGATT AATGTTTTTG ATGGTAAAGC CCAGAAAAAC 
CAAGGTGACC AGTTGCAAAA CTGTGATAAA TCTCTGCCAC GAAACATCTC CTTAAAGAAA 
GCGAAGAAAC CTCCCCTTCC CCCTTTCAGA ACAGATTCCC TTCGAAGGTC CCCCAAGAAA 
AAAACTCACC CCAATGGGCA GGTGCTAAAT GAGACTCTGA TTGCTACCCT CCAACATTCC 
CTACAACTGA ATCTGCAGCG GAAAGATAGT AGTTCTCCTT CCCAGAGCCC CTGTAGCGAT 
TACGAAGATC CATGGCTCCT CCGTTCCCGT AGCCAGAGCA CAGTCAGCAC GAGCAGCAGC 
ATGGTGTCGG CCACAGCACC AAATGTGTAT TCACTTTGCG CGGTCACCCC ATCTCAGAGC 
GACACAAGCA GCATCAAGTC AGAGTACGGA GATCCTTGGG GTTACTACAT CGACTATACT 
GGCGTACAAG AAGATCAGGG GAAATCCAGT GGCACAGGTC CACCTAATGC TGTGACTCCA 
TTGTGTGATC TTGCCACCAG CCATCTCCAG GATGGATGCA GGGTGCCTGT CCCACAAGTG 
TCTAGTGGAT TGGCCAAACC AAAGAACGCT TCCCCAGAGA AGTCTCACAG GGTCACTTCC 
CCATCTAGTG GCTATTCCAG CCAATCGAAT ACCCCCACAG CGCTTACTCC AGTGCCTGTG 
TTTTTAAAAT CTATCTCACC AGGAAATGGG AAATCAAAGT TGAAGCCCAA AGTGCCTGAA 
AGAAAATCCT CCCTGATATC TTCAGTATCT ATCTCTTCAT CTTCAACTTC CCTTTCTTCC 
AATACTTCAA CTGAAGGCAG TGTGAATATG AAGAAATTAG ATTCAGCTGT AAGCACTCCT 
CCCATTCCTC CTCTTTCCCC AGCATCTGTA CATCCTCTTA ATCACTCCCC TCTCCTTCCT 
CCTCCTCCTT TAGCAGATGC CTTGGAACTG TCCACTGTAC AGCATTCTCC CATTTTTCCT 
CCTCCACCAC CAGAAGTCCT TGCTTCTTCT TTTCTTCCAA CGGATACATG CTTTCCTCTT 
CCCCCAGCAT CCTTCGGTAT GCCCAATTCA ACTGCTCCCT TGCCACCTCC TCCTTCATCT 
CTCTTTCCCT CTTCTGTGCC TCCACCTGCT CCACCTCTTG ACCCTAAGTT GATGAAAGAA 
GCTTGGCCCT CTTTAAAAAA GTGTGGCCAA CAGGATTCTT CTTGTGCTGT ACTTAAACCG 
TCTCTCACCA AGGAAGAAAC CACTAAACCA CCAATGCCCC TAATAACCAC TAAAGCATTA 
CAGATGGTAC GGTTGAGGCC TGTGAACAAG CCCTCAGAAA CATCAGGAGC TCAGCCAGGC 
AAACCAGCTT CTGAAACAGA ATCTCAGGAA AATAATTCTG TGGTGCCACA GGATCCTTTA 
AAACCATCTT CTGCCTTGAA ACACAGAGGG AGCATCAATG GACTAGACAG CAGCAAAGCC 
CAAAATGTCC CCGTTAAAAA CACACCTCCA ACTCCTGAGA AGGGCTCGTT TTGGGGTTGG 
CACAGCAGGA CAGCTGAGCT TGCTGCTCTT CAAAGGCATG GGGATGCAGC TGATCCTGCA 
CCATCCTCCG AAGCATCCTT TTCAGGAACA AATGCTGAGG AAGGAGGGCA GGTTTCTGGG 
CAGGACGAGG AATCTCCTTC TGGCCTCAGG ACTGCATTGC TCCAGGAGCA GCCCAGCTCT 
CCAAAGAAGA CACATGGCTC TTCTTCACCC AGCAGGAAAC CACCTCCTAT TTCAAAGAAA 
CCCAAACTGT TCCTTGTCGT GCCACCTCCG CACATAGATT TCACAGCAGA GAAAACAACC 
AAAGTGAATG AAAGTGTCAG CAGCCCAGCC AGGGGAGAAG TGGTTGATGT GAATGGCAAA 
GAGGCTCAAG AGAGCTTTGC TGCAGGACCT GGTTCTGCCG AGACAGACTT TGGCAGCTTG 
GTGCCAGGGG GTGGAGCTGT GGGATCCACC TTCCTTGCAG GAGTGGGAGC CGATGTCTAT 
ATGGTACAGC CTGATGTATC ACCAGCTCAA GGAGCTCCCA AGCAGGAGGA GCCAACCACT 
TCAGATGAAG GCAGCAGGAT AGAGAGCACT GAAGACAGTG GTGGTAACGT GGGCAGTCCC 
CCCTCTCGAG AGGATGGTAA CATTGGGGTA TTTGAGGCTG ACATGGTATG TACCTCTCCT 
GGACCATGCA GTAGCCTTAA GGAAGATGGG GGTGATGAGG TGACAACTCC AAGTAGACCC 
AGAACTACAG AAGACCTTTT TGCAGCTATC CACAGGTCTA AGAGGAAAGT CCTTGGCCGA 
AAGGATTCTG ATGATGATCA TTCCCGAAAC CATTCTCCAT CACCTCCAGT GACACCCACT 
GGGGCTGCCC CCAGCCTGGC TTCCCTTAAG CAAGCAGGAT CCATCCAGAG AAGCATTCGT 
AAGAGTAGTA CTAGCAGTGA TAACTTCAAA GCTCTGCTGC TGAAAAAGGG GAGCCGGACA 
GATGCCAGCT CTCGAATGTC AGCGGCTGAG ATGCTGAAGA ATACAGACCC AAGGTTTCAC 
AGATCCAAGT CGGAGTCCTC GTCAGACACC CCTGACAGCC CCTCAAGCTG TTCTCCAAAC 
AAGAACAGGA GGGCCCAGGA GGAGTGGGCC AAGAATGAAG GCCTGATGCC AAGGAGTCTG 
TCCTTCTCTG GCACCAGGTA TGGCCGGAGC CGGACACCGC CTTCTGCTGC AAGTAGCAGG 
TACAGCATGA GGAACCGAAT TCAAAGCAGC CCCATGACTG TCATTTCTGA GGGAGAAGGG 
GAAGCAACAG AGCCCACAGA GAACAGACTC CATCGGACTG CAGGAGTTGG AAGGGAAAAA 
GAAATGGATG GATTTACTGG GAATGAAATG GACACTAGTG ATCTGCCATA TTCTGAAGAG 
TGTGGCTCCA AGGGAGCTCA GGCTCCTAAC CATGTTGACT CCACAGATAA GACAGCTAGT 
AAGACTCTAT CAGGACAATG TAGAAGTTCT TTAAGGGAAG AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484687.1
CDS	187..5334
Translation	MPFHKRTVEPVKMCRLDEAAGDEAAGVSGRCGSGPGSGKLGRAPQPSAGAAGGAATAEGAGAGAGGLLFTSLEQVSAHSLIFLLWQLSDLSRCAGDIFAEIEGQATALGRRAARLHGRLGALQAAASRLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSKSSLACDTYKDEDEESDQKCSISSSEEEKLIATRRARTPTSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQTVQTDVVPINVTATGTSQNDFRGHSMYVPDHYSTLGRLDNYRPAIQRSETRDSSCQTDEVKVVPPSMRRIRAQKGQGIAAQMSQFSVSSGNMSVMSDSAGVVFPPRFHSDVGFHSLPRPGTRLSLQPLESPQAVENPAEDLAGILAYQMGKLQVDENFEHARCISTPGTLSRPKSEELRHYESEKTSPACIVSPHAAYSTSLIANATISSSSEVIAIHTVQNTGQLDSRIASSSPYARMMKSRDNLATSSAIKDDHRSSSGNWSESSSTRHSQTSDTITSNVVMMASLCDSDASLNTPGNKENGSRNVAYNSRNNLNFLSHSQDSDGKSESSYSGDRAPGGTEPWEYKASGSERASPHKSHCITPGYSTPASNMSSSSLDQASVKEDANSLYSVDHDGYYTSMHMDSGIKSRNRCNSSDGFGNPRHSVINVFDGKAQKNQGDQLQNCDKSLPRNISLKKAKKPPLPPFRTDSLRRSPKKKTHPNGQVLNETLIATLQHSLQLNLQRKDSSSPSQSPCSDYEDPWLLRSRSQSTVSTSSSMVSATAPNVYSLCAVTPSQSDTSSIKSEYGDPWGYYIDYTGVQEDQGKSSGTGPPNAVTPLCDLATSHLQDGCRVPVPQVSSGLAKPKNASPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSISPGNGKSKLKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSVNMKKLDSAVSTPPIPPLSPASVHPLNHSPLLPPPPLADALELSTVQHSPIFPPPPPEVLASSFLPTDTCFPLPPASFGMPNSTAPLPPPPSSLFPSSVPPPAPPLDPKLMKEAWPSLKKCGQQDSSCAVLKPSLTKEETTKPPMPLITTKALQMVRLRPVNKPSETSGAQPGKPASETESQENNSVVPQDPLKPSSALKHRGSINGLDSSKAQNVPVKNTPPTPEKGSFWGWHSRTAELAALQRHGDAADPAPSSEASFSGTNAEEGGQVSGQDEESPSGLRTALLQEQPSSPKKTHGSSSPSRKPPPISKKPKLFLVVPPPHIDFTAEKTTKVNESVSSPARGEVVDVNGKEAQESFAAGPGSAETDFGSLVPGGGAVGSTFLAGVGADVYMVQPDVSPAQGAPKQEEPTTSDEGSRIESTEDSGGNVGSPPSREDGNIGVFEADMVCTSPGPCSSLKEDGGDEVTTPSRPRTTEDLFAAIHRSKRKVLGRKDSDDDHSRNHSPSPPVTPTGAAPSLASLKQAGSIQRSIRKSSTSSDNFKALLLKKGSRTDASSRMSAAEMLKNTDPRFHRSKSESSSDTPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGTRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEATEPTENRLHRTAGVGREKEMDGFTGNEMDTSDLPYSEECGSKGAQAPNHVDSTDKTASKTLSGQCRSSLREES

Target ORF information:

RefSeq Version	XM_007484625.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007484625.2

ORF Insert Sequence:

ATGCCTTTTC ACAAGAGGAC TGTGGAGCCC GTAAAGATGT GCCGGCTGGA CGAAGCTGCC 
GGAGACGAGG CTGCCGGGGT CTCCGGGCGA TGCGGAAGCG GTCCCGGGTC AGGGAAGCTG 
GGGCGGGCGC CGCAGCCGTC GGCGGGCGCT GCGGGAGGAG CCGCTACGGC CGAAGGAGCC 
GGGGCTGGGG CCGGGGGGCT GCTCTTCACT TCCCTGGAGC AGGTCAGCGC GCACTCTCTG 
ATTTTCTTGC TCTGGCAGCT CTCTGACCTC TCCCGTTGCG CGGGGGACAT CTTCGCCGAG 
ATAGAGGGGC AGGCAACTGC CCTGGGTCGC CGGGCCGCCC GGCTCCACGG CCGACTGGGC 
GCTCTGCAAG CCGCGGCCAG CAGACTGGAC CACCGCAAAG TCAAAATCCC TGTCTCCAAT 
TTAGATGAAG AGAGTAGATG GACAGTTCAT TACACAGCAC CATGGCATCA GCAGGAGAAT 
GTGTTCCTAC CCACCACAAG GCCACCCTGT GTGGAAGACT TGCATCGACA AGCCAAGCTG 
AACCTCAAAT CAGTACTGAG AGAATGTGAC AAACTGAGGA GAGATGGTTA TCGAAGCTCT 
CAGTACTACT CTCAGGGACC TACTTTCTCT AAATCCAGCT TGGCCTGTGA CACCTACAAA 
GATGAAGATG AAGAAAGTGA TCAGAAGTGT TCCATCTCTT CCTCAGAGGA AGAAAAACTC 
ATTGCTACCA GGAGAGCCAG AACTCCCACT TCAAATGATT TATCTGATAT CAACACGCAG 
ACAAATTGGA CCAAGTCACT TCCCCTGCCA ACACCAGAGG AAAAGATGAG ACAGCAAGCA 
CAAACAGTCC AGACAGATGT GGTTCCTATT AATGTAACTG CTACTGGCAC TAGCCAGAAT 
GACTTCCGTG GTCACTCGAT GTATGTCCCC GACCACTACT CCACACTAGG AAGGCTTGAC 
AACTATCGAC CCGCCATTCA GCGTTCTGAA ACCAGGGACT CTAGTTGTCA GACTGATGAA 
GTAAAAGTTG TGCCCCCTTC CATGAGAAGA ATCAGGGCAC AAAAGGGGCA AGGTATTGCT 
GCCCAGATGA GCCAGTTCTC CGTCTCATCA GGGAACATGT CAGTGATGAG CGATTCTGCA 
GGTGTTGTGT TTCCTCCACG CTTCCACAGT GATGTGGGTT TCCATAGCCT TCCTCGTCCT 
GGAACAAGGT TGTCCCTTCA ACCTCTCGAG TCACCCCAGG CTGTTGAGAA CCCTGCAGAG 
GACTTGGCTG GCATTCTCGC CTACCAGATG GGTAAGCTGC AAGTTGATGA AAACTTTGAG 
CATGCGAGAT GTATTTCAAC ACCTGGGACA TTATCGAGAC CTAAATCAGA AGAGTTGAGA 
CATTATGAAA GTGAAAAAAC AAGCCCAGCC TGTATTGTTT CTCCTCATGC TGCCTACTCT 
ACCAGCCTCA TAGCAAATGC CACAATTTCT TCATCATCGG AGGTTATTGC TATACACACA 
GTCCAGAATA CTGGACAGCT GGACAGCAGA ATCGCCAGCT CATCCCCTTA TGCCAGGATG 
ATGAAGTCCA GAGACAATCT TGCCACCAGC AGTGCCATAA AAGATGATCA TCGGTCTTCC 
AGTGGTAACT GGAGCGAGAG TAGCTCGACA CGACACTCAC AGACTTCAGA CACCATTACC 
AGCAATGTAG TCATGATGGC GTCCCTCTGT GACTCTGATG CCTCTCTGAA CACTCCAGGA 
AACAAGGAGA ATGGGTCCCG AAATGTAGCC TACAACAGCA GAAACAACCT GAATTTCCTA 
AGCCATTCCC AAGACAGCGA TGGTAAGAGT GAGTCCAGTT ACTCAGGTGA CAGAGCACCT 
GGGGGCACAG AGCCCTGGGA ATATAAAGCA TCAGGCAGTG AGAGGGCATC CCCACACAAG 
TCACATTGTA TTACTCCTGG CTACTCCACT CCTGCTAGTA ACATGAGCAG CAGTAGTTTA 
GACCAAGCCT CAGTCAAAGA GGATGCAAAT TCTCTCTACT CAGTAGACCA TGATGGCTAC 
TACACTTCCA TGCACATGGA CTCTGGAATC AAATCAAGAA ATCGGTGCAA TAGCAGTGAT 
GGCTTTGGGA ACCCCAGGCA CAGTGTGATT AATGTTTTTG ATGGTAAAGC CCAGAAAAAC 
CAAGGTGACC AGTTGCAAAA CTGTGATAAA TCTCTGCCAC GAAACATCTC CTTAAAGAAA 
GCGAAGAAAC CTCCCCTTCC CCCTTTCAGA ACAGATTCCC TTCGAAGGTC CCCCAAGAAA 
AAAACTCACC CCAATGGGCA GGTGCTAAAT GAGACTCTGA TTGCTACCCT CCAACATTCC 
CTACAACTGA ATCTGCAGCG GAAAGATAGT AGTTCTCCTT CCCAGAGCCC CTGTAGCGAT 
TACGAAGATC CATGGCTCCT CCGTTCCCGT AGCCAGAGCA CAGTCAGCAC GAGCAGCAGC 
ATGGTGTCGG CCACAGCACC AAATGTGTAT TCACTTTGCG CGGTCACCCC ATCTCAGAGC 
GACACAAGCA GCATCAAGTC AGAGTACGGA GATCCTTGGG GTTACTACAT CGACTATACT 
GGCGTACAAG AAGATCAGGG GAAATCCAGT GGCACAGGTC CACCTAATGC TGTGACTCCA 
TTGTGTGATC TTGCCACCAG CCATCTCCAG GATGGATGCA GGGTGCCTGT CCCACAAGTG 
TCTAGTGGAT TGGCCAAACC AAAGAACGCT TCCCCAGAGA AGTCTCACAG GGTCACTTCC 
CCATCTAGTG GCTATTCCAG CCAATCGAAT ACCCCCACAG CGCTTACTCC AGTGCCTGTG 
TTTTTAAAAT CTATCTCACC AGGAAATGGG AAATCAAAGT TGAAGCCCAA AGTGCCTGAA 
AGAAAATCCT CCCTGATATC TTCAGTATCT ATCTCTTCAT CTTCAACTTC CCTTTCTTCC 
AATACTTCAA CTGAAGGCAG TGTGAATATG AAGAAATTAG ATTCAGCTGT AAGCACTCCT 
CCCATTCCTC CTCTTTCCCC AGCATCTGTA CATCCTCTTA ATCACTCCCC TCTCCTTCCT 
CCTCCTCCTT TAGCAGATGC CTTGGAACTG TCCACTGTAC AGCATTCTCC CATTTTTCCT 
CCTCCACCAC CAGAAGTCCT TGCTTCTTCT TTTCTTCCAA CGGATACATG CTTTCCTCTT 
CCCCCAGCAT CCTTCGGTAT GCCCAATTCA ACTGCTCCCT TGCCACCTCC TCCTTCATCT 
CTCTTTCCCT CTTCTGTGCC TCCACCTGCT CCACCTCTTG ACCCTAAGTT GATGAAAGAA 
GCTTGGCCCT CTTTAAAAAA GTGTGGCCAA CAGGATTCTT CTTGTGCTGT ACTTAAACCG 
TCTCTCACCA AGGAAGAAAC CACTAAACCA CCAATGCCCC TAATAACCAC TAAAGCATTA 
CAGATGGTAC GGTTGAGGCC TGTGAACAAG CCCTCAGAAA CATCAGGAGC TCAGCCAGGC 
AAACCAGCTT CTGAAACAGA ATCTCAGGAA AATAATTCTG TGGTGCCACA GGATCCTTTA 
AAACCATCTT CTGCCTTGAA ACACAGAGGG AGCATCAATG GACTAGACAG CAGCAAAGCC 
CAAAATGTCC CCGTTAAAAA CACACCTCCA ACTCCTGAGA AGGGCTCGTT TTGGGGTTGG 
CACAGCAGGA CAGCTGAGCT TGCTGCTCTT CAAAGGCATG GGGATGCAGC TGATCCTGCA 
CCATCCTCCG AAGCATCCTT TTCAGGAACA AATGCTGAGG AAGGAGGGCA GGTTTCTGGG 
CAGGACGAGG AATCTCCTTC TGGCCTCAGG ACTGCATTGC TCCAGGAGCA GCCCAGCTCT 
CCAAAGAAGA CACATGGCTC TTCTTCACCC AGCAGGAAAC CACCTCCTAT TTCAAAGAAA 
CCCAAACTGT TCCTTGTCGT GCCACCTCCG CACATAGATT TCACAGCAGA GAAAACAACC 
AAAGTGAATG AAAGTGTCAG CAGCCCAGCC AGGGGAGAAG TGGTTGATGT GAATGGCAAA 
GAGGCTCAAG AGAGCTTTGC TGCAGGACCT GGTTCTGCCG AGACAGACTT TGGCAGCTTG 
GTGCCAGGGG GTGGAGCTGT GGGATCCACC TTCCTTGCAG GAGTGGGAGC CGATGTCTAT 
ATGGTACAGC CTGATGTATC ACCAGCTCAA GGAGCTCCCA AGCAGGAGGA GCCAACCACT 
TCAGATGAAG GCAGCAGGAT AGAGAGCACT GAAGACAGTG GTGGTAACGT GGGCAGTCCC 
CCCTCTCGAG AGGATGGTAA CATTGGGGTA TTTGAGGCTG ACATGGTATG TACCTCTCCT 
GGACCATGCA GTAGCCTTAA GGAAGATGGG GGTGATGAGG TGACAACTCC AAGTAGACCC 
AGAACTACAG AAGACCTTTT TGCAGCTATC CACAGGTCTA AGAGGAAAGT CCTTGGCCGA 
AAGGATTCTG ATGATGATCA TTCCCGAAAC CATTCTCCAT CACCTCCAGT GACACCCACT 
GGGGCTGCCC CCAGCCTGGC TTCCCTTAAG CAAGCAGGAT CCATCCAGAG AAGCATTCGT 
AAGAGTAGTA CTAGCAGTGA TAACTTCAAA GCTCTGCTGC TGAAAAAGGG GAGCCGGACA 
GATGCCAGCT CTCGAATGTC AGCGGCTGAG ATGCTGAAGA ATACAGACCC AAGGTTTCAC 
AGATCCAAGT CGGAGTCCTC GTCAGACACC CCTGACAGCC CCTCAAGCTG TTCTCCAAAC 
AAGAACAGGA GGGCCCAGGA GGAGTGGGCC AAGAATGAAG GCCTGATGCC AAGGAGTCTG 
TCCTTCTCTG GCACCAGGTA TGGCCGGAGC CGGACACCGC CTTCTGCTGC AAGTAGCAGG 
TACAGCATGA GGAACCGAAT TCAAAGCAGC CCCATGACTG TCATTTCTGA GGGAGAAGGG 
GAAGCAACAG AGCCCACAGA GAACAGACTC CATCGGACTG CAGGAGTTGG AAGGGAAAAA 
GAAATGGATG GATTTACTGG GAATGAAATG GACACTAGTG ATCTGCCATA TTCTGAAGAG 
TGTGGCTCCA AGGGAGCTCA GGCTCCTAAC CATGTTGACT CCACAGATAA GACAGCTAGT 
AAGACTCTAT CAGGACAATG TAGAAGTTCT TTAAGGGAAG AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_007484626.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484688.1 Monodelphis domestica NHS like 1 (NHSL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMc12985
Clone ID Related Accession (Same CDS sequence)	XM_007484626.2
Accession Version	XM_007484626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007484626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AATGTGCTTC AGGTTCCTTC AGACTCAAGC CAAACTCAGG TTCTCTCTCA 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCCAAGC AGACAAAGAG GTTGTTTCGC 
AGTGATGGGG AGCTCTCAAT ATGTGGGCAG CAGGTAGAAG ATGATGATGA AAATTGGATC 
TACAGAACTC AGCACAGAAA AGCTGTCTCC AATTTAGATG AAGAGAGTAG ATGGACAGTT 
CATTACACAG CACCATGGCA TCAGCAGGAG AATGTGTTCC TACCCACCAC AAGGCCACCC 
TGTGTGGAAG ACTTGCATCG ACAAGCCAAG CTGAACCTCA AATCAGTACT GAGAGAATGT 
GACAAACTGA GGAGAGATGG TTATCGAAGC TCTCAGTACT ACTCTCAGGG ACCTACTTTC 
TCTAAATCCA GCTTGGCCTG TGACACCTAC AAAGATGAAG ATGAAGAAAG TGATCAGAAG 
TGTTCCATCT CTTCCTCAGA GGAAGAAAAA CTCATTGCTA CCAGGAGAGC CAGAACTCCC 
ACTTCAAATG ATTTATCTGA TATCAACACG CAGACAAATT GGACCAAGTC ACTTCCCCTG 
CCAACACCAG AGGAAAAGAT GAGACAGCAA GCACAAACAG TCCAGACAGA TGTGGTTCCT 
ATTAATGTAA CTGGGGAGAA TTTCGACCGC CAAGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACAGACACTG TCGTAAGACG GCCGAAGAAA GTCAAAAGGA GAAAGACTAT TACAGGAATC 
CCTGACATCA TACAAAAGGA GCTAGCTACT GGCACTAGCC AGAATGACTT CCGTGGTCAC 
TCGATGTATG TCCCCGACCA CTACTCCACA CTAGGAAGGC TTGACAACTA TCGACCCGCC 
ATTCAGCGTT CTGAAACCAG GGACTCTAGT TGTCAGACTG ATGAAGTAAA AGTTGTGCCC 
CCTTCCATGA GAAGAATCAG GGCACAAAAG GGGCAAGGTA TTGCTGCCCA GATGAGCCAG 
TTCTCCGTCT CATCAGGGAA CATGTCAGTG ATGAGCGATT CTGCAGGTGT TGTGTTTCCT 
CCACGCTTCC ACAGTGATGT GGGTTTCCAT AGCCTTCCTC GTCCTGGAAC AAGGTTGTCC 
CTTCAACCTC TCGAGTCACC CCAGGCTGTT GAGAACCCTG CAGAGGACTT GGCTGGCATT 
CTCGCCTACC AGATGGGTAA GCTGCAAGTT GATGAAAACT TTGAGCATGC GAGATGTATT 
TCAACACCTG GGACATTATC GAGACCTAAA TCAGAAGAGT TGAGACATTA TGAAAGTGAA 
AAAACAAGCC CAGCCTGTAT TGTTTCTCCT CATGCTGCCT ACTCTACCAG CCTCATAGCA 
AATGCCACAA TTTCTTCATC ATCGGAGGTT ATTGCTATAC ACACAGTCCA GAATACTGGA 
CAGCTGGACA GCAGAATCGC CAGCTCATCC CCTTATGCCA GGATGATGAA GTCCAGAGAC 
AATCTTGCCA CCAGCAGTGC CATAAAAGAT GATCATCGGT CTTCCAGTGG TAACTGGAGC 
GAGAGTAGCT CGACACGACA CTCACAGACT TCAGACACCA TTACCAGCAA TGTAGTCATG 
ATGGCGTCCC TCTGTGACTC TGATGCCTCT CTGAACACTC CAGGAAACAA GGAGAATGGG 
TCCCGAAATG TAGCCTACAA CAGCAGAAAC AACCTGAATT TCCTAAGCCA TTCCCAAGAC 
AGCGATGGTA AGAGTGAGTC CAGTTACTCA GGTGACAGAG CACCTGGGGG CACAGAGCCC 
TGGGAATATA AAGCATCAGG CAGTGAGAGG GCATCCCCAC ACAAGTCACA TTGTATTACT 
CCTGGCTACT CCACTCCTGC TAGTAACATG AGCAGCAGTA GTTTAGACCA AGCCTCAGTC 
AAAGAGGATG CAAATTCTCT CTACTCAGTA GACCATGATG GCTACTACAC TTCCATGCAC 
ATGGACTCTG GAATCAAATC AAGAAATCGG TGCAATAGCA GTGATGGCTT TGGGAACCCC 
AGGCACAGTG TGATTAATGT TTTTGATGGT AAAGCCCAGA AAAACCAAGG TGACCAGTTG 
CAAAACTGTG ATAAATCTCT GCCACGAAAC ATCTCCTTAA AGAAAGCGAA GAAACCTCCC 
CTTCCCCCTT TCAGAACAGA TTCCCTTCGA AGGTCCCCCA AGAAAAAAAC TCACCCCAAT 
GGGCAGGTGC TAAATGAGAC TCTGATTGCT ACCCTCCAAC ATTCCCTACA ACTGAATCTG 
CAGCGGAAAG ATAGTAGTTC TCCTTCCCAG AGCCCCTGTA GCGATTACGA AGATCCATGG 
CTCCTCCGTT CCCGTAGCCA GAGCACAGTC AGCACGAGCA GCAGCATGGT GTCGGCCACA 
GCACCAAATG TGTATTCACT TTGCGCGGTC ACCCCATCTC AGAGCGACAC AAGCAGCATC 
AAGTCAGAGT ACGGAGATCC TTGGGGTTAC TACATCGACT ATACTGGCGT ACAAGAAGAT 
CAGGGGAAAT CCAGTGGCAC AGGTCCACCT AATGCTGTGA CTCCATTGTG TGATCTTGCC 
ACCAGCCATC TCCAGGATGG ATGCAGGGTG CCTGTCCCAC AAGTGTCTAG TGGATTGGCC 
AAACCAAAGA ACGCTTCCCC AGAGAAGTCT CACAGGGTCA CTTCCCCATC TAGTGGCTAT 
TCCAGCCAAT CGAATACCCC CACAGCGCTT ACTCCAGTGC CTGTGTTTTT AAAATCTATC 
TCACCAGGAA ATGGGAAATC AAAGTTGAAG CCCAAAGTGC CTGAAAGAAA ATCCTCCCTG 
ATATCTTCAG TATCTATCTC TTCATCTTCA ACTTCCCTTT CTTCCAATAC TTCAACTGAA 
GGCAGTGTGA ATATGAAGAA ATTAGATTCA GCTGTAAGCA CTCCTCCCAT TCCTCCTCTT 
TCCCCAGCAT CTGTACATCC TCTTAATCAC TCCCCTCTCC TTCCTCCTCC TCCTTTAGCA 
GATGCCTTGG AACTGTCCAC TGTACAGCAT TCTCCCATTT TTCCTCCTCC ACCACCAGAA 
GTCCTTGCTT CTTCTTTTCT TCCAACGGAT ACATGCTTTC CTCTTCCCCC AGCATCCTTC 
GGTATGCCCA ATTCAACTGC TCCCTTGCCA CCTCCTCCTT CATCTCTCTT TCCCTCTTCT 
GTGCCTCCAC CTGCTCCACC TCTTGACCCT AAGTTGATGA AAGAAGCTTG GCCCTCTTTA 
AAAAAGTGTG GCCAACAGGA TTCTTCTTGT GCTGTACTTA AACCGTCTCT CACCAAGGAA 
GAAACCACTA AACCACCAAT GCCCCTAATA ACCACTAAAG CATTACAGAT GGTACGGTTG 
AGGCCTGTGA ACAAGCCCTC AGAAACATCA GGAGCTCAGC CAGGCAAACC AGCTTCTGAA 
ACAGAATCTC AGGAAAATAA TTCTGTGGTG CCACAGGATC CTTTAAAACC ATCTTCTGCC 
TTGAAACACA GAGGGAGCAT CAATGGACTA GACAGCAGCA AAGCCCAAAA TGTCCCCGTT 
AAAAACACAC CTCCAACTCC TGAGAAGGGC TCGTTTTGGG GTTGGCACAG CAGGACAGCT 
GAGCTTGCTG CTCTTCAAAG GCATGGGGAT GCAGCTGATC CTGCACCATC CTCCGAAGCA 
TCCTTTTCAG GAACAAATGC TGAGGAAGGA GGGCAGGTTT CTGGGCAGGA CGAGGAATCT 
CCTTCTGGCC TCAGGACTGC ATTGCTCCAG GAGCAGCCCA GCTCTCCAAA GAAGACACAT 
GGCTCTTCTT CACCCAGCAG GAAACCACCT CCTATTTCAA AGAAACCCAA ACTGTTCCTT 
GTCGTGCCAC CTCCGCACAT AGATTTCACA GCAGAGAAAA CAACCAAAGT GAATGAAAGT 
GTCAGCAGCC CAGCCAGGGG AGAAGTGGTT GATGTGAATG GCAAAGAGGC TCAAGAGAGC 
TTTGCTGCAG GACCTGGTTC TGCCGAGACA GACTTTGGCA GCTTGGTGCC AGGGGGTGGA 
GCTGTGGGAT CCACCTTCCT TGCAGGAGTG GGAGCCGATG TCTATATGGT ACAGCCTGAT 
GTATCACCAG CTCAAGGAGC TCCCAAGCAG GAGGAGCCAA CCACTTCAGA TGAAGGCAGC 
AGGATAGAGA GCACTGAAGA CAGTGGTGGT AACGTGGGCA GTCCCCCCTC TCGAGAGGAT 
GGTAACATTG GGGTATTTGA GGCTGACATG GTATGTACCT CTCCTGGACC ATGCAGTAGC 
CTTAAGGAAG ATGGGGGTGA TGAGGTGACA ACTCCAAGTA GACCCAGAAC TACAGAAGAC 
CTTTTTGCAG CTATCCACAG GTCTAAGAGG AAAGTCCTTG GCCGAAAGGA TTCTGATGAT 
GATCATTCCC GAAACCATTC TCCATCACCT CCAGTGACAC CCACTGGGGC TGCCCCCAGC 
CTGGCTTCCC TTAAGCAAGC AGGATCCATC CAGAGAAGCA TTCGTAAGAG TAGTACTAGC 
AGTGATAACT TCAAAGCTCT GCTGCTGAAA AAGGGGAGCC GGACAGATGC CAGCTCTCGA 
ATGTCAGCGG CTGAGATGCT GAAGAATACA GACCCAAGGT TTCACAGATC CAAGTCGGAG 
TCCTCGTCAG ACACCCCTGA CAGCCCCTCA AGCTGTTCTC CAAACAAGAA CAGGAGGGCC 
CAGGAGGAGT GGGCCAAGAA TGAAGGCCTG ATGCCAAGGA GTCTGTCCTT CTCTGGCACC 
AGGTATGGCC GGAGCCGGAC ACCGCCTTCT GCTGCAAGTA GCAGGTACAG CATGAGGAAC 
CGAATTCAAA GCAGCCCCAT GACTGTCATT TCTGAGGGAG AAGGGGAAGC AACAGAGCCC 
ACAGAGAACA GACTCCATCG GACTGCAGGA GTTGGAAGGG AAAAAGAAAT GGATGGATTT 
ACTGGGAATG AAATGGACAC TAGTGATCTG CCATATTCTG AAGAGTGTGG CTCCAAGGGA 
GCTCAGGCTC CTAACCATGT TGACTCCACA GATAAGACAG CTAGTAAGAC TCTATCAGGA 
CAATGTAGAA GTTCTTTAAG GGAAGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484688.1
CDS	774..5846
Translation	MKKECASGSFRLKPNSGSLSRAVSWINFSSLSKQTKRLFRSDGELSICGQQVEDDDENWIYRTQHRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSKSSLACDTYKDEDEESDQKCSISSSEEEKLIATRRARTPTSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQTVQTDVVPINVTGENFDRQASLRRSLIYTDTVVRRPKKVKRRKTITGIPDIIQKELATGTSQNDFRGHSMYVPDHYSTLGRLDNYRPAIQRSETRDSSCQTDEVKVVPPSMRRIRAQKGQGIAAQMSQFSVSSGNMSVMSDSAGVVFPPRFHSDVGFHSLPRPGTRLSLQPLESPQAVENPAEDLAGILAYQMGKLQVDENFEHARCISTPGTLSRPKSEELRHYESEKTSPACIVSPHAAYSTSLIANATISSSSEVIAIHTVQNTGQLDSRIASSSPYARMMKSRDNLATSSAIKDDHRSSSGNWSESSSTRHSQTSDTITSNVVMMASLCDSDASLNTPGNKENGSRNVAYNSRNNLNFLSHSQDSDGKSESSYSGDRAPGGTEPWEYKASGSERASPHKSHCITPGYSTPASNMSSSSLDQASVKEDANSLYSVDHDGYYTSMHMDSGIKSRNRCNSSDGFGNPRHSVINVFDGKAQKNQGDQLQNCDKSLPRNISLKKAKKPPLPPFRTDSLRRSPKKKTHPNGQVLNETLIATLQHSLQLNLQRKDSSSPSQSPCSDYEDPWLLRSRSQSTVSTSSSMVSATAPNVYSLCAVTPSQSDTSSIKSEYGDPWGYYIDYTGVQEDQGKSSGTGPPNAVTPLCDLATSHLQDGCRVPVPQVSSGLAKPKNASPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSISPGNGKSKLKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSVNMKKLDSAVSTPPIPPLSPASVHPLNHSPLLPPPPLADALELSTVQHSPIFPPPPPEVLASSFLPTDTCFPLPPASFGMPNSTAPLPPPPSSLFPSSVPPPAPPLDPKLMKEAWPSLKKCGQQDSSCAVLKPSLTKEETTKPPMPLITTKALQMVRLRPVNKPSETSGAQPGKPASETESQENNSVVPQDPLKPSSALKHRGSINGLDSSKAQNVPVKNTPPTPEKGSFWGWHSRTAELAALQRHGDAADPAPSSEASFSGTNAEEGGQVSGQDEESPSGLRTALLQEQPSSPKKTHGSSSPSRKPPPISKKPKLFLVVPPPHIDFTAEKTTKVNESVSSPARGEVVDVNGKEAQESFAAGPGSAETDFGSLVPGGGAVGSTFLAGVGADVYMVQPDVSPAQGAPKQEEPTTSDEGSRIESTEDSGGNVGSPPSREDGNIGVFEADMVCTSPGPCSSLKEDGGDEVTTPSRPRTTEDLFAAIHRSKRKVLGRKDSDDDHSRNHSPSPPVTPTGAAPSLASLKQAGSIQRSIRKSSTSSDNFKALLLKKGSRTDASSRMSAAEMLKNTDPRFHRSKSESSSDTPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGTRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEATEPTENRLHRTAGVGREKEMDGFTGNEMDTSDLPYSEECGSKGAQAPNHVDSTDKTASKTLSGQCRSSLREES

Target ORF information:

RefSeq Version	XM_007484626.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007484626.2

ORF Insert Sequence:

ATGAAAAAGG AATGTGCTTC AGGTTCCTTC AGACTCAAGC CAAACTCAGG TTCTCTCTCA 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCCAAGC AGACAAAGAG GTTGTTTCGC 
AGTGATGGGG AGCTCTCAAT ATGTGGGCAG CAGGTAGAAG ATGATGATGA AAATTGGATC 
TACAGAACTC AGCACAGAAA AGCTGTCTCC AATTTAGATG AAGAGAGTAG ATGGACAGTT 
CATTACACAG CACCATGGCA TCAGCAGGAG AATGTGTTCC TACCCACCAC AAGGCCACCC 
TGTGTGGAAG ACTTGCATCG ACAAGCCAAG CTGAACCTCA AATCAGTACT GAGAGAATGT 
GACAAACTGA GGAGAGATGG TTATCGAAGC TCTCAGTACT ACTCTCAGGG ACCTACTTTC 
TCTAAATCCA GCTTGGCCTG TGACACCTAC AAAGATGAAG ATGAAGAAAG TGATCAGAAG 
TGTTCCATCT CTTCCTCAGA GGAAGAAAAA CTCATTGCTA CCAGGAGAGC CAGAACTCCC 
ACTTCAAATG ATTTATCTGA TATCAACACG CAGACAAATT GGACCAAGTC ACTTCCCCTG 
CCAACACCAG AGGAAAAGAT GAGACAGCAA GCACAAACAG TCCAGACAGA TGTGGTTCCT 
ATTAATGTAA CTGGGGAGAA TTTCGACCGC CAAGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACAGACACTG TCGTAAGACG GCCGAAGAAA GTCAAAAGGA GAAAGACTAT TACAGGAATC 
CCTGACATCA TACAAAAGGA GCTAGCTACT GGCACTAGCC AGAATGACTT CCGTGGTCAC 
TCGATGTATG TCCCCGACCA CTACTCCACA CTAGGAAGGC TTGACAACTA TCGACCCGCC 
ATTCAGCGTT CTGAAACCAG GGACTCTAGT TGTCAGACTG ATGAAGTAAA AGTTGTGCCC 
CCTTCCATGA GAAGAATCAG GGCACAAAAG GGGCAAGGTA TTGCTGCCCA GATGAGCCAG 
TTCTCCGTCT CATCAGGGAA CATGTCAGTG ATGAGCGATT CTGCAGGTGT TGTGTTTCCT 
CCACGCTTCC ACAGTGATGT GGGTTTCCAT AGCCTTCCTC GTCCTGGAAC AAGGTTGTCC 
CTTCAACCTC TCGAGTCACC CCAGGCTGTT GAGAACCCTG CAGAGGACTT GGCTGGCATT 
CTCGCCTACC AGATGGGTAA GCTGCAAGTT GATGAAAACT TTGAGCATGC GAGATGTATT 
TCAACACCTG GGACATTATC GAGACCTAAA TCAGAAGAGT TGAGACATTA TGAAAGTGAA 
AAAACAAGCC CAGCCTGTAT TGTTTCTCCT CATGCTGCCT ACTCTACCAG CCTCATAGCA 
AATGCCACAA TTTCTTCATC ATCGGAGGTT ATTGCTATAC ACACAGTCCA GAATACTGGA 
CAGCTGGACA GCAGAATCGC CAGCTCATCC CCTTATGCCA GGATGATGAA GTCCAGAGAC 
AATCTTGCCA CCAGCAGTGC CATAAAAGAT GATCATCGGT CTTCCAGTGG TAACTGGAGC 
GAGAGTAGCT CGACACGACA CTCACAGACT TCAGACACCA TTACCAGCAA TGTAGTCATG 
ATGGCGTCCC TCTGTGACTC TGATGCCTCT CTGAACACTC CAGGAAACAA GGAGAATGGG 
TCCCGAAATG TAGCCTACAA CAGCAGAAAC AACCTGAATT TCCTAAGCCA TTCCCAAGAC 
AGCGATGGTA AGAGTGAGTC CAGTTACTCA GGTGACAGAG CACCTGGGGG CACAGAGCCC 
TGGGAATATA AAGCATCAGG CAGTGAGAGG GCATCCCCAC ACAAGTCACA TTGTATTACT 
CCTGGCTACT CCACTCCTGC TAGTAACATG AGCAGCAGTA GTTTAGACCA AGCCTCAGTC 
AAAGAGGATG CAAATTCTCT CTACTCAGTA GACCATGATG GCTACTACAC TTCCATGCAC 
ATGGACTCTG GAATCAAATC AAGAAATCGG TGCAATAGCA GTGATGGCTT TGGGAACCCC 
AGGCACAGTG TGATTAATGT TTTTGATGGT AAAGCCCAGA AAAACCAAGG TGACCAGTTG 
CAAAACTGTG ATAAATCTCT GCCACGAAAC ATCTCCTTAA AGAAAGCGAA GAAACCTCCC 
CTTCCCCCTT TCAGAACAGA TTCCCTTCGA AGGTCCCCCA AGAAAAAAAC TCACCCCAAT 
GGGCAGGTGC TAAATGAGAC TCTGATTGCT ACCCTCCAAC ATTCCCTACA ACTGAATCTG 
CAGCGGAAAG ATAGTAGTTC TCCTTCCCAG AGCCCCTGTA GCGATTACGA AGATCCATGG 
CTCCTCCGTT CCCGTAGCCA GAGCACAGTC AGCACGAGCA GCAGCATGGT GTCGGCCACA 
GCACCAAATG TGTATTCACT TTGCGCGGTC ACCCCATCTC AGAGCGACAC AAGCAGCATC 
AAGTCAGAGT ACGGAGATCC TTGGGGTTAC TACATCGACT ATACTGGCGT ACAAGAAGAT 
CAGGGGAAAT CCAGTGGCAC AGGTCCACCT AATGCTGTGA CTCCATTGTG TGATCTTGCC 
ACCAGCCATC TCCAGGATGG ATGCAGGGTG CCTGTCCCAC AAGTGTCTAG TGGATTGGCC 
AAACCAAAGA ACGCTTCCCC AGAGAAGTCT CACAGGGTCA CTTCCCCATC TAGTGGCTAT 
TCCAGCCAAT CGAATACCCC CACAGCGCTT ACTCCAGTGC CTGTGTTTTT AAAATCTATC 
TCACCAGGAA ATGGGAAATC AAAGTTGAAG CCCAAAGTGC CTGAAAGAAA ATCCTCCCTG 
ATATCTTCAG TATCTATCTC TTCATCTTCA ACTTCCCTTT CTTCCAATAC TTCAACTGAA 
GGCAGTGTGA ATATGAAGAA ATTAGATTCA GCTGTAAGCA CTCCTCCCAT TCCTCCTCTT 
TCCCCAGCAT CTGTACATCC TCTTAATCAC TCCCCTCTCC TTCCTCCTCC TCCTTTAGCA 
GATGCCTTGG AACTGTCCAC TGTACAGCAT TCTCCCATTT TTCCTCCTCC ACCACCAGAA 
GTCCTTGCTT CTTCTTTTCT TCCAACGGAT ACATGCTTTC CTCTTCCCCC AGCATCCTTC 
GGTATGCCCA ATTCAACTGC TCCCTTGCCA CCTCCTCCTT CATCTCTCTT TCCCTCTTCT 
GTGCCTCCAC CTGCTCCACC TCTTGACCCT AAGTTGATGA AAGAAGCTTG GCCCTCTTTA 
AAAAAGTGTG GCCAACAGGA TTCTTCTTGT GCTGTACTTA AACCGTCTCT CACCAAGGAA 
GAAACCACTA AACCACCAAT GCCCCTAATA ACCACTAAAG CATTACAGAT GGTACGGTTG 
AGGCCTGTGA ACAAGCCCTC AGAAACATCA GGAGCTCAGC CAGGCAAACC AGCTTCTGAA 
ACAGAATCTC AGGAAAATAA TTCTGTGGTG CCACAGGATC CTTTAAAACC ATCTTCTGCC 
TTGAAACACA GAGGGAGCAT CAATGGACTA GACAGCAGCA AAGCCCAAAA TGTCCCCGTT 
AAAAACACAC CTCCAACTCC TGAGAAGGGC TCGTTTTGGG GTTGGCACAG CAGGACAGCT 
GAGCTTGCTG CTCTTCAAAG GCATGGGGAT GCAGCTGATC CTGCACCATC CTCCGAAGCA 
TCCTTTTCAG GAACAAATGC TGAGGAAGGA GGGCAGGTTT CTGGGCAGGA CGAGGAATCT 
CCTTCTGGCC TCAGGACTGC ATTGCTCCAG GAGCAGCCCA GCTCTCCAAA GAAGACACAT 
GGCTCTTCTT CACCCAGCAG GAAACCACCT CCTATTTCAA AGAAACCCAA ACTGTTCCTT 
GTCGTGCCAC CTCCGCACAT AGATTTCACA GCAGAGAAAA CAACCAAAGT GAATGAAAGT 
GTCAGCAGCC CAGCCAGGGG AGAAGTGGTT GATGTGAATG GCAAAGAGGC TCAAGAGAGC 
TTTGCTGCAG GACCTGGTTC TGCCGAGACA GACTTTGGCA GCTTGGTGCC AGGGGGTGGA 
GCTGTGGGAT CCACCTTCCT TGCAGGAGTG GGAGCCGATG TCTATATGGT ACAGCCTGAT 
GTATCACCAG CTCAAGGAGC TCCCAAGCAG GAGGAGCCAA CCACTTCAGA TGAAGGCAGC 
AGGATAGAGA GCACTGAAGA CAGTGGTGGT AACGTGGGCA GTCCCCCCTC TCGAGAGGAT 
GGTAACATTG GGGTATTTGA GGCTGACATG GTATGTACCT CTCCTGGACC ATGCAGTAGC 
CTTAAGGAAG ATGGGGGTGA TGAGGTGACA ACTCCAAGTA GACCCAGAAC TACAGAAGAC 
CTTTTTGCAG CTATCCACAG GTCTAAGAGG AAAGTCCTTG GCCGAAAGGA TTCTGATGAT 
GATCATTCCC GAAACCATTC TCCATCACCT CCAGTGACAC CCACTGGGGC TGCCCCCAGC 
CTGGCTTCCC TTAAGCAAGC AGGATCCATC CAGAGAAGCA TTCGTAAGAG TAGTACTAGC 
AGTGATAACT TCAAAGCTCT GCTGCTGAAA AAGGGGAGCC GGACAGATGC CAGCTCTCGA 
ATGTCAGCGG CTGAGATGCT GAAGAATACA GACCCAAGGT TTCACAGATC CAAGTCGGAG 
TCCTCGTCAG ACACCCCTGA CAGCCCCTCA AGCTGTTCTC CAAACAAGAA CAGGAGGGCC 
CAGGAGGAGT GGGCCAAGAA TGAAGGCCTG ATGCCAAGGA GTCTGTCCTT CTCTGGCACC 
AGGTATGGCC GGAGCCGGAC ACCGCCTTCT GCTGCAAGTA GCAGGTACAG CATGAGGAAC 
CGAATTCAAA GCAGCCCCAT GACTGTCATT TCTGAGGGAG AAGGGGAAGC AACAGAGCCC 
ACAGAGAACA GACTCCATCG GACTGCAGGA GTTGGAAGGG AAAAAGAAAT GGATGGATTT 
ACTGGGAATG AAATGGACAC TAGTGATCTG CCATATTCTG AAGAGTGTGG CTCCAAGGGA 
GCTCAGGCTC CTAACCATGT TGACTCCACA GATAAGACAG CTAGTAAGAC TCTATCAGGA 
CAATGTAGAA GTTCTTTAAG GGAAGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_007484627.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484689.1 Monodelphis domestica NHS like 1 (NHSL1), transcript variant X4, mRNA.

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CloneID	OMc12986
Clone ID Related Accession (Same CDS sequence)	XM_007484627.2
Accession Version	XM_007484627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007484627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AATGTGCTTC AGGTTCCTTC AGACTCAAGC CAAACTCAGG TTCTCTCTCA 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCCAAGC AGACAAAGAG GTTGTTTCGC 
AGTGATGGGG AGCTCTCAAT ATGTGGGCAG CAGGTAGAAG ATGATGATGA AAATTGGATC 
TACAGAACTC AGCACAGAAA AGCTGTCTCC AATTTAGATG AAGAGAGTAG ATGGACAGTT 
CATTACACAG CACCATGGCA TCAGCAGGAG AATGTGTTCC TACCCACCAC AAGGCCACCC 
TGTGTGGAAG ACTTGCATCG ACAAGCCAAG CTGAACCTCA AATCAGTACT GAGAGAATGT 
GACAAACTGA GGAGAGATGG TTATCGAAGC TCTCAGTACT ACTCTCAGGG ACCTACTTTC 
TCTAAATCCA GCTTGGCCTG TGACACCTAC AAAGATGAAG ATGAAGAAAG TGATCAGAAG 
TGTTCCATCT CTTCCTCAGA GGAAGAAAAA CTCATTGCTA CCAGGAGAGC CAGAACTCCC 
ACTTCAAATG ATTTATCTGA TATCAACACG CAGACAAATT GGACCAAGTC ACTTCCCCTG 
CCAACACCAG AGGAAAAGAT GAGACAGCAA GCACAAACAG TCCAGACAGA TGTGGTTCCT 
ATTAATGTAA CTGCTACTGG CACTAGCCAG AATGACTTCC GTGGTCACTC GATGTATGTC 
CCCGACCACT ACTCCACACT AGGAAGGCTT GACAACTATC GACCCGCCAT TCAGCGTTCT 
GAAACCAGGG ACTCTAGTTG TCAGACTGAT GAAGTAAAAG TTGTGCCCCC TTCCATGAGA 
AGAATCAGGG CACAAAAGGG GCAAGGTATT GCTGCCCAGA TGAGCCAGTT CTCCGTCTCA 
TCAGGGAACA TGTCAGTGAT GAGCGATTCT GCAGGTGTTG TGTTTCCTCC ACGCTTCCAC 
AGTGATGTGG GTTTCCATAG CCTTCCTCGT CCTGGAACAA GGTTGTCCCT TCAACCTCTC 
GAGTCACCCC AGGCTGTTGA GAACCCTGCA GAGGACTTGG CTGGCATTCT CGCCTACCAG 
ATGGGTAAGC TGCAAGTTGA TGAAAACTTT GAGCATGCGA GATGTATTTC AACACCTGGG 
ACATTATCGA GACCTAAATC AGAAGAGTTG AGACATTATG AAAGTGAAAA AACAAGCCCA 
GCCTGTATTG TTTCTCCTCA TGCTGCCTAC TCTACCAGCC TCATAGCAAA TGCCACAATT 
TCTTCATCAT CGGAGGTTAT TGCTATACAC ACAGTCCAGA ATACTGGACA GCTGGACAGC 
AGAATCGCCA GCTCATCCCC TTATGCCAGG ATGATGAAGT CCAGAGACAA TCTTGCCACC 
AGCAGTGCCA TAAAAGATGA TCATCGGTCT TCCAGTGGTA ACTGGAGCGA GAGTAGCTCG 
ACACGACACT CACAGACTTC AGACACCATT ACCAGCAATG TAGTCATGAT GGCGTCCCTC 
TGTGACTCTG ATGCCTCTCT GAACACTCCA GGAAACAAGG AGAATGGGTC CCGAAATGTA 
GCCTACAACA GCAGAAACAA CCTGAATTTC CTAAGCCATT CCCAAGACAG CGATGGTAAG 
AGTGAGTCCA GTTACTCAGG TGACAGAGCA CCTGGGGGCA CAGAGCCCTG GGAATATAAA 
GCATCAGGCA GTGAGAGGGC ATCCCCACAC AAGTCACATT GTATTACTCC TGGCTACTCC 
ACTCCTGCTA GTAACATGAG CAGCAGTAGT TTAGACCAAG CCTCAGTCAA AGAGGATGCA 
AATTCTCTCT ACTCAGTAGA CCATGATGGC TACTACACTT CCATGCACAT GGACTCTGGA 
ATCAAATCAA GAAATCGGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGTGTG 
ATTAATGTTT TTGATGGTAA AGCCCAGAAA AACCAAGGTG ACCAGTTGCA AAACTGTGAT 
AAATCTCTGC CACGAAACAT CTCCTTAAAG AAAGCGAAGA AACCTCCCCT TCCCCCTTTC 
AGAACAGATT CCCTTCGAAG GTCCCCCAAG AAAAAAACTC ACCCCAATGG GCAGGTGCTA 
AATGAGACTC TGATTGCTAC CCTCCAACAT TCCCTACAAC TGAATCTGCA GCGGAAAGAT 
AGTAGTTCTC CTTCCCAGAG CCCCTGTAGC GATTACGAAG ATCCATGGCT CCTCCGTTCC 
CGTAGCCAGA GCACAGTCAG CACGAGCAGC AGCATGGTGT CGGCCACAGC ACCAAATGTG 
TATTCACTTT GCGCGGTCAC CCCATCTCAG AGCGACACAA GCAGCATCAA GTCAGAGTAC 
GGAGATCCTT GGGGTTACTA CATCGACTAT ACTGGCGTAC AAGAAGATCA GGGGAAATCC 
AGTGGCACAG GTCCACCTAA TGCTGTGACT CCATTGTGTG ATCTTGCCAC CAGCCATCTC 
CAGGATGGAT GCAGGGTGCC TGTCCCACAA GTGTCTAGTG GATTGGCCAA ACCAAAGAAC 
GCTTCCCCAG AGAAGTCTCA CAGGGTCACT TCCCCATCTA GTGGCTATTC CAGCCAATCG 
AATACCCCCA CAGCGCTTAC TCCAGTGCCT GTGTTTTTAA AATCTATCTC ACCAGGAAAT 
GGGAAATCAA AGTTGAAGCC CAAAGTGCCT GAAAGAAAAT CCTCCCTGAT ATCTTCAGTA 
TCTATCTCTT CATCTTCAAC TTCCCTTTCT TCCAATACTT CAACTGAAGG CAGTGTGAAT 
ATGAAGAAAT TAGATTCAGC TGTAAGCACT CCTCCCATTC CTCCTCTTTC CCCAGCATCT 
GTACATCCTC TTAATCACTC CCCTCTCCTT CCTCCTCCTC CTTTAGCAGA TGCCTTGGAA 
CTGTCCACTG TACAGCATTC TCCCATTTTT CCTCCTCCAC CACCAGAAGT CCTTGCTTCT 
TCTTTTCTTC CAACGGATAC ATGCTTTCCT CTTCCCCCAG CATCCTTCGG TATGCCCAAT 
TCAACTGCTC CCTTGCCACC TCCTCCTTCA TCTCTCTTTC CCTCTTCTGT GCCTCCACCT 
GCTCCACCTC TTGACCCTAA GTTGATGAAA GAAGCTTGGC CCTCTTTAAA AAAGTGTGGC 
CAACAGGATT CTTCTTGTGC TGTACTTAAA CCGTCTCTCA CCAAGGAAGA AACCACTAAA 
CCACCAATGC CCCTAATAAC CACTAAAGCA TTACAGATGG TACGGTTGAG GCCTGTGAAC 
AAGCCCTCAG AAACATCAGG AGCTCAGCCA GGCAAACCAG CTTCTGAAAC AGAATCTCAG 
GAAAATAATT CTGTGGTGCC ACAGGATCCT TTAAAACCAT CTTCTGCCTT GAAACACAGA 
GGGAGCATCA ATGGACTAGA CAGCAGCAAA GCCCAAAATG TCCCCGTTAA AAACACACCT 
CCAACTCCTG AGAAGGGCTC GTTTTGGGGT TGGCACAGCA GGACAGCTGA GCTTGCTGCT 
CTTCAAAGGC ATGGGGATGC AGCTGATCCT GCACCATCCT CCGAAGCATC CTTTTCAGGA 
ACAAATGCTG AGGAAGGAGG GCAGGTTTCT GGGCAGGACG AGGAATCTCC TTCTGGCCTC 
AGGACTGCAT TGCTCCAGGA GCAGCCCAGC TCTCCAAAGA AGACACATGG CTCTTCTTCA 
CCCAGCAGGA AACCACCTCC TATTTCAAAG AAACCCAAAC TGTTCCTTGT CGTGCCACCT 
CCGCACATAG ATTTCACAGC AGAGAAAACA ACCAAAGTGA ATGAAAGTGT CAGCAGCCCA 
GCCAGGGGAG AAGTGGTTGA TGTGAATGGC AAAGAGGCTC AAGAGAGCTT TGCTGCAGGA 
CCTGGTTCTG CCGAGACAGA CTTTGGCAGC TTGGTGCCAG GGGGTGGAGC TGTGGGATCC 
ACCTTCCTTG CAGGAGTGGG AGCCGATGTC TATATGGTAC AGCCTGATGT ATCACCAGCT 
CAAGGAGCTC CCAAGCAGGA GGAGCCAACC ACTTCAGATG AAGGCAGCAG GATAGAGAGC 
ACTGAAGACA GTGGTGGTAA CGTGGGCAGT CCCCCCTCTC GAGAGGATGG TAACATTGGG 
GTATTTGAGG CTGACATGGT ATGTACCTCT CCTGGACCAT GCAGTAGCCT TAAGGAAGAT 
GGGGGTGATG AGGTGACAAC TCCAAGTAGA CCCAGAACTA CAGAAGACCT TTTTGCAGCT 
ATCCACAGGT CTAAGAGGAA AGTCCTTGGC CGAAAGGATT CTGATGATGA TCATTCCCGA 
AACCATTCTC CATCACCTCC AGTGACACCC ACTGGGGCTG CCCCCAGCCT GGCTTCCCTT 
AAGCAAGCAG GATCCATCCA GAGAAGCATT CGTAAGAGTA GTACTAGCAG TGATAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGGAGCCGG ACAGATGCCA GCTCTCGAAT GTCAGCGGCT 
GAGATGCTGA AGAATACAGA CCCAAGGTTT CACAGATCCA AGTCGGAGTC CTCGTCAGAC 
ACCCCTGACA GCCCCTCAAG CTGTTCTCCA AACAAGAACA GGAGGGCCCA GGAGGAGTGG 
GCCAAGAATG AAGGCCTGAT GCCAAGGAGT CTGTCCTTCT CTGGCACCAG GTATGGCCGG 
AGCCGGACAC CGCCTTCTGC TGCAAGTAGC AGGTACAGCA TGAGGAACCG AATTCAAAGC 
AGCCCCATGA CTGTCATTTC TGAGGGAGAA GGGGAAGCAA CAGAGCCCAC AGAGAACAGA 
CTCCATCGGA CTGCAGGAGT TGGAAGGGAA AAAGAAATGG ATGGATTTAC TGGGAATGAA 
ATGGACACTA GTGATCTGCC ATATTCTGAA GAGTGTGGCT CCAAGGGAGC TCAGGCTCCT 
AACCATGTTG ACTCCACAGA TAAGACAGCT AGTAAGACTC TATCAGGACA ATGTAGAAGT 
TCTTTAAGGG AAGAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484689.1
CDS	774..5714
Translation	MKKECASGSFRLKPNSGSLSRAVSWINFSSLSKQTKRLFRSDGELSICGQQVEDDDENWIYRTQHRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSKSSLACDTYKDEDEESDQKCSISSSEEEKLIATRRARTPTSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQTVQTDVVPINVTATGTSQNDFRGHSMYVPDHYSTLGRLDNYRPAIQRSETRDSSCQTDEVKVVPPSMRRIRAQKGQGIAAQMSQFSVSSGNMSVMSDSAGVVFPPRFHSDVGFHSLPRPGTRLSLQPLESPQAVENPAEDLAGILAYQMGKLQVDENFEHARCISTPGTLSRPKSEELRHYESEKTSPACIVSPHAAYSTSLIANATISSSSEVIAIHTVQNTGQLDSRIASSSPYARMMKSRDNLATSSAIKDDHRSSSGNWSESSSTRHSQTSDTITSNVVMMASLCDSDASLNTPGNKENGSRNVAYNSRNNLNFLSHSQDSDGKSESSYSGDRAPGGTEPWEYKASGSERASPHKSHCITPGYSTPASNMSSSSLDQASVKEDANSLYSVDHDGYYTSMHMDSGIKSRNRCNSSDGFGNPRHSVINVFDGKAQKNQGDQLQNCDKSLPRNISLKKAKKPPLPPFRTDSLRRSPKKKTHPNGQVLNETLIATLQHSLQLNLQRKDSSSPSQSPCSDYEDPWLLRSRSQSTVSTSSSMVSATAPNVYSLCAVTPSQSDTSSIKSEYGDPWGYYIDYTGVQEDQGKSSGTGPPNAVTPLCDLATSHLQDGCRVPVPQVSSGLAKPKNASPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSISPGNGKSKLKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSVNMKKLDSAVSTPPIPPLSPASVHPLNHSPLLPPPPLADALELSTVQHSPIFPPPPPEVLASSFLPTDTCFPLPPASFGMPNSTAPLPPPPSSLFPSSVPPPAPPLDPKLMKEAWPSLKKCGQQDSSCAVLKPSLTKEETTKPPMPLITTKALQMVRLRPVNKPSETSGAQPGKPASETESQENNSVVPQDPLKPSSALKHRGSINGLDSSKAQNVPVKNTPPTPEKGSFWGWHSRTAELAALQRHGDAADPAPSSEASFSGTNAEEGGQVSGQDEESPSGLRTALLQEQPSSPKKTHGSSSPSRKPPPISKKPKLFLVVPPPHIDFTAEKTTKVNESVSSPARGEVVDVNGKEAQESFAAGPGSAETDFGSLVPGGGAVGSTFLAGVGADVYMVQPDVSPAQGAPKQEEPTTSDEGSRIESTEDSGGNVGSPPSREDGNIGVFEADMVCTSPGPCSSLKEDGGDEVTTPSRPRTTEDLFAAIHRSKRKVLGRKDSDDDHSRNHSPSPPVTPTGAAPSLASLKQAGSIQRSIRKSSTSSDNFKALLLKKGSRTDASSRMSAAEMLKNTDPRFHRSKSESSSDTPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGTRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEATEPTENRLHRTAGVGREKEMDGFTGNEMDTSDLPYSEECGSKGAQAPNHVDSTDKTASKTLSGQCRSSLREES

Target ORF information:

RefSeq Version	XM_007484627.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007484627.2

ORF Insert Sequence:

ATGAAAAAGG AATGTGCTTC AGGTTCCTTC AGACTCAAGC CAAACTCAGG TTCTCTCTCA 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCCAAGC AGACAAAGAG GTTGTTTCGC 
AGTGATGGGG AGCTCTCAAT ATGTGGGCAG CAGGTAGAAG ATGATGATGA AAATTGGATC 
TACAGAACTC AGCACAGAAA AGCTGTCTCC AATTTAGATG AAGAGAGTAG ATGGACAGTT 
CATTACACAG CACCATGGCA TCAGCAGGAG AATGTGTTCC TACCCACCAC AAGGCCACCC 
TGTGTGGAAG ACTTGCATCG ACAAGCCAAG CTGAACCTCA AATCAGTACT GAGAGAATGT 
GACAAACTGA GGAGAGATGG TTATCGAAGC TCTCAGTACT ACTCTCAGGG ACCTACTTTC 
TCTAAATCCA GCTTGGCCTG TGACACCTAC AAAGATGAAG ATGAAGAAAG TGATCAGAAG 
TGTTCCATCT CTTCCTCAGA GGAAGAAAAA CTCATTGCTA CCAGGAGAGC CAGAACTCCC 
ACTTCAAATG ATTTATCTGA TATCAACACG CAGACAAATT GGACCAAGTC ACTTCCCCTG 
CCAACACCAG AGGAAAAGAT GAGACAGCAA GCACAAACAG TCCAGACAGA TGTGGTTCCT 
ATTAATGTAA CTGCTACTGG CACTAGCCAG AATGACTTCC GTGGTCACTC GATGTATGTC 
CCCGACCACT ACTCCACACT AGGAAGGCTT GACAACTATC GACCCGCCAT TCAGCGTTCT 
GAAACCAGGG ACTCTAGTTG TCAGACTGAT GAAGTAAAAG TTGTGCCCCC TTCCATGAGA 
AGAATCAGGG CACAAAAGGG GCAAGGTATT GCTGCCCAGA TGAGCCAGTT CTCCGTCTCA 
TCAGGGAACA TGTCAGTGAT GAGCGATTCT GCAGGTGTTG TGTTTCCTCC ACGCTTCCAC 
AGTGATGTGG GTTTCCATAG CCTTCCTCGT CCTGGAACAA GGTTGTCCCT TCAACCTCTC 
GAGTCACCCC AGGCTGTTGA GAACCCTGCA GAGGACTTGG CTGGCATTCT CGCCTACCAG 
ATGGGTAAGC TGCAAGTTGA TGAAAACTTT GAGCATGCGA GATGTATTTC AACACCTGGG 
ACATTATCGA GACCTAAATC AGAAGAGTTG AGACATTATG AAAGTGAAAA AACAAGCCCA 
GCCTGTATTG TTTCTCCTCA TGCTGCCTAC TCTACCAGCC TCATAGCAAA TGCCACAATT 
TCTTCATCAT CGGAGGTTAT TGCTATACAC ACAGTCCAGA ATACTGGACA GCTGGACAGC 
AGAATCGCCA GCTCATCCCC TTATGCCAGG ATGATGAAGT CCAGAGACAA TCTTGCCACC 
AGCAGTGCCA TAAAAGATGA TCATCGGTCT TCCAGTGGTA ACTGGAGCGA GAGTAGCTCG 
ACACGACACT CACAGACTTC AGACACCATT ACCAGCAATG TAGTCATGAT GGCGTCCCTC 
TGTGACTCTG ATGCCTCTCT GAACACTCCA GGAAACAAGG AGAATGGGTC CCGAAATGTA 
GCCTACAACA GCAGAAACAA CCTGAATTTC CTAAGCCATT CCCAAGACAG CGATGGTAAG 
AGTGAGTCCA GTTACTCAGG TGACAGAGCA CCTGGGGGCA CAGAGCCCTG GGAATATAAA 
GCATCAGGCA GTGAGAGGGC ATCCCCACAC AAGTCACATT GTATTACTCC TGGCTACTCC 
ACTCCTGCTA GTAACATGAG CAGCAGTAGT TTAGACCAAG CCTCAGTCAA AGAGGATGCA 
AATTCTCTCT ACTCAGTAGA CCATGATGGC TACTACACTT CCATGCACAT GGACTCTGGA 
ATCAAATCAA GAAATCGGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGTGTG 
ATTAATGTTT TTGATGGTAA AGCCCAGAAA AACCAAGGTG ACCAGTTGCA AAACTGTGAT 
AAATCTCTGC CACGAAACAT CTCCTTAAAG AAAGCGAAGA AACCTCCCCT TCCCCCTTTC 
AGAACAGATT CCCTTCGAAG GTCCCCCAAG AAAAAAACTC ACCCCAATGG GCAGGTGCTA 
AATGAGACTC TGATTGCTAC CCTCCAACAT TCCCTACAAC TGAATCTGCA GCGGAAAGAT 
AGTAGTTCTC CTTCCCAGAG CCCCTGTAGC GATTACGAAG ATCCATGGCT CCTCCGTTCC 
CGTAGCCAGA GCACAGTCAG CACGAGCAGC AGCATGGTGT CGGCCACAGC ACCAAATGTG 
TATTCACTTT GCGCGGTCAC CCCATCTCAG AGCGACACAA GCAGCATCAA GTCAGAGTAC 
GGAGATCCTT GGGGTTACTA CATCGACTAT ACTGGCGTAC AAGAAGATCA GGGGAAATCC 
AGTGGCACAG GTCCACCTAA TGCTGTGACT CCATTGTGTG ATCTTGCCAC CAGCCATCTC 
CAGGATGGAT GCAGGGTGCC TGTCCCACAA GTGTCTAGTG GATTGGCCAA ACCAAAGAAC 
GCTTCCCCAG AGAAGTCTCA CAGGGTCACT TCCCCATCTA GTGGCTATTC CAGCCAATCG 
AATACCCCCA CAGCGCTTAC TCCAGTGCCT GTGTTTTTAA AATCTATCTC ACCAGGAAAT 
GGGAAATCAA AGTTGAAGCC CAAAGTGCCT GAAAGAAAAT CCTCCCTGAT ATCTTCAGTA 
TCTATCTCTT CATCTTCAAC TTCCCTTTCT TCCAATACTT CAACTGAAGG CAGTGTGAAT 
ATGAAGAAAT TAGATTCAGC TGTAAGCACT CCTCCCATTC CTCCTCTTTC CCCAGCATCT 
GTACATCCTC TTAATCACTC CCCTCTCCTT CCTCCTCCTC CTTTAGCAGA TGCCTTGGAA 
CTGTCCACTG TACAGCATTC TCCCATTTTT CCTCCTCCAC CACCAGAAGT CCTTGCTTCT 
TCTTTTCTTC CAACGGATAC ATGCTTTCCT CTTCCCCCAG CATCCTTCGG TATGCCCAAT 
TCAACTGCTC CCTTGCCACC TCCTCCTTCA TCTCTCTTTC CCTCTTCTGT GCCTCCACCT 
GCTCCACCTC TTGACCCTAA GTTGATGAAA GAAGCTTGGC CCTCTTTAAA AAAGTGTGGC 
CAACAGGATT CTTCTTGTGC TGTACTTAAA CCGTCTCTCA CCAAGGAAGA AACCACTAAA 
CCACCAATGC CCCTAATAAC CACTAAAGCA TTACAGATGG TACGGTTGAG GCCTGTGAAC 
AAGCCCTCAG AAACATCAGG AGCTCAGCCA GGCAAACCAG CTTCTGAAAC AGAATCTCAG 
GAAAATAATT CTGTGGTGCC ACAGGATCCT TTAAAACCAT CTTCTGCCTT GAAACACAGA 
GGGAGCATCA ATGGACTAGA CAGCAGCAAA GCCCAAAATG TCCCCGTTAA AAACACACCT 
CCAACTCCTG AGAAGGGCTC GTTTTGGGGT TGGCACAGCA GGACAGCTGA GCTTGCTGCT 
CTTCAAAGGC ATGGGGATGC AGCTGATCCT GCACCATCCT CCGAAGCATC CTTTTCAGGA 
ACAAATGCTG AGGAAGGAGG GCAGGTTTCT GGGCAGGACG AGGAATCTCC TTCTGGCCTC 
AGGACTGCAT TGCTCCAGGA GCAGCCCAGC TCTCCAAAGA AGACACATGG CTCTTCTTCA 
CCCAGCAGGA AACCACCTCC TATTTCAAAG AAACCCAAAC TGTTCCTTGT CGTGCCACCT 
CCGCACATAG ATTTCACAGC AGAGAAAACA ACCAAAGTGA ATGAAAGTGT CAGCAGCCCA 
GCCAGGGGAG AAGTGGTTGA TGTGAATGGC AAAGAGGCTC AAGAGAGCTT TGCTGCAGGA 
CCTGGTTCTG CCGAGACAGA CTTTGGCAGC TTGGTGCCAG GGGGTGGAGC TGTGGGATCC 
ACCTTCCTTG CAGGAGTGGG AGCCGATGTC TATATGGTAC AGCCTGATGT ATCACCAGCT 
CAAGGAGCTC CCAAGCAGGA GGAGCCAACC ACTTCAGATG AAGGCAGCAG GATAGAGAGC 
ACTGAAGACA GTGGTGGTAA CGTGGGCAGT CCCCCCTCTC GAGAGGATGG TAACATTGGG 
GTATTTGAGG CTGACATGGT ATGTACCTCT CCTGGACCAT GCAGTAGCCT TAAGGAAGAT 
GGGGGTGATG AGGTGACAAC TCCAAGTAGA CCCAGAACTA CAGAAGACCT TTTTGCAGCT 
ATCCACAGGT CTAAGAGGAA AGTCCTTGGC CGAAAGGATT CTGATGATGA TCATTCCCGA 
AACCATTCTC CATCACCTCC AGTGACACCC ACTGGGGCTG CCCCCAGCCT GGCTTCCCTT 
AAGCAAGCAG GATCCATCCA GAGAAGCATT CGTAAGAGTA GTACTAGCAG TGATAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGGAGCCGG ACAGATGCCA GCTCTCGAAT GTCAGCGGCT 
GAGATGCTGA AGAATACAGA CCCAAGGTTT CACAGATCCA AGTCGGAGTC CTCGTCAGAC 
ACCCCTGACA GCCCCTCAAG CTGTTCTCCA AACAAGAACA GGAGGGCCCA GGAGGAGTGG 
GCCAAGAATG AAGGCCTGAT GCCAAGGAGT CTGTCCTTCT CTGGCACCAG GTATGGCCGG 
AGCCGGACAC CGCCTTCTGC TGCAAGTAGC AGGTACAGCA TGAGGAACCG AATTCAAAGC 
AGCCCCATGA CTGTCATTTC TGAGGGAGAA GGGGAAGCAA CAGAGCCCAC AGAGAACAGA 
CTCCATCGGA CTGCAGGAGT TGGAAGGGAA AAAGAAATGG ATGGATTTAC TGGGAATGAA 
ATGGACACTA GTGATCTGCC ATATTCTGAA GAGTGTGGCT CCAAGGGAGC TCAGGCTCCT 
AACCATGTTG ACTCCACAGA TAAGACAGCT AGTAAGACTC TATCAGGACA ATGTAGAAGT 
TCTTTAAGGG AAGAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_007484628.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484690.1 Monodelphis domestica NHS like 1 (NHSL1), transcript variant X5, mRNA.

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CloneID	OMc12987
Clone ID Related Accession (Same CDS sequence)	XM_007484628.2
Accession Version	XM_007484628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007484628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCATAT TCATTAACAC AAAGATCAAG TCTCTGATTA AACTTTTTAA GAAGAAGACT 
GTCTCCAATT TAGATGAAGA GAGTAGATGG ACAGTTCATT ACACAGCACC ATGGCATCAG 
CAGGAGAATG TGTTCCTACC CACCACAAGG CCACCCTGTG TGGAAGACTT GCATCGACAA 
GCCAAGCTGA ACCTCAAATC AGTACTGAGA GAATGTGACA AACTGAGGAG AGATGGTTAT 
CGAAGCTCTC AGTACTACTC TCAGGGACCT ACTTTCTCTA AATCCAGCTT GGCCTGTGAC 
ACCTACAAAG ATGAAGATGA AGAAAGTGAT CAGAAGTGTT CCATCTCTTC CTCAGAGGAA 
GAAAAACTCA TTGCTACCAG GAGAGCCAGA ACTCCCACTT CAAATGATTT ATCTGATATC 
AACACGCAGA CAAATTGGAC CAAGTCACTT CCCCTGCCAA CACCAGAGGA AAAGATGAGA 
CAGCAAGCAC AAACAGTCCA GACAGATGTG GTTCCTATTA ATGTAACTGG GGAGAATTTC 
GACCGCCAAG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTGTCGT AAGACGGCCG 
AAGAAAGTCA AAAGGAGAAA GACTATTACA GGAATCCCTG ACATCATACA AAAGGAGCTA 
GCTACTGGCA CTAGCCAGAA TGACTTCCGT GGTCACTCGA TGTATGTCCC CGACCACTAC 
TCCACACTAG GAAGGCTTGA CAACTATCGA CCCGCCATTC AGCGTTCTGA AACCAGGGAC 
TCTAGTTGTC AGACTGATGA AGTAAAAGTT GTGCCCCCTT CCATGAGAAG AATCAGGGCA 
CAAAAGGGGC AAGGTATTGC TGCCCAGATG AGCCAGTTCT CCGTCTCATC AGGGAACATG 
TCAGTGATGA GCGATTCTGC AGGTGTTGTG TTTCCTCCAC GCTTCCACAG TGATGTGGGT 
TTCCATAGCC TTCCTCGTCC TGGAACAAGG TTGTCCCTTC AACCTCTCGA GTCACCCCAG 
GCTGTTGAGA ACCCTGCAGA GGACTTGGCT GGCATTCTCG CCTACCAGAT GGGTAAGCTG 
CAAGTTGATG AAAACTTTGA GCATGCGAGA TGTATTTCAA CACCTGGGAC ATTATCGAGA 
CCTAAATCAG AAGAGTTGAG ACATTATGAA AGTGAAAAAA CAAGCCCAGC CTGTATTGTT 
TCTCCTCATG CTGCCTACTC TACCAGCCTC ATAGCAAATG CCACAATTTC TTCATCATCG 
GAGGTTATTG CTATACACAC AGTCCAGAAT ACTGGACAGC TGGACAGCAG AATCGCCAGC 
TCATCCCCTT ATGCCAGGAT GATGAAGTCC AGAGACAATC TTGCCACCAG CAGTGCCATA 
AAAGATGATC ATCGGTCTTC CAGTGGTAAC TGGAGCGAGA GTAGCTCGAC ACGACACTCA 
CAGACTTCAG ACACCATTAC CAGCAATGTA GTCATGATGG CGTCCCTCTG TGACTCTGAT 
GCCTCTCTGA ACACTCCAGG AAACAAGGAG AATGGGTCCC GAAATGTAGC CTACAACAGC 
AGAAACAACC TGAATTTCCT AAGCCATTCC CAAGACAGCG ATGGTAAGAG TGAGTCCAGT 
TACTCAGGTG ACAGAGCACC TGGGGGCACA GAGCCCTGGG AATATAAAGC ATCAGGCAGT 
GAGAGGGCAT CCCCACACAA GTCACATTGT ATTACTCCTG GCTACTCCAC TCCTGCTAGT 
AACATGAGCA GCAGTAGTTT AGACCAAGCC TCAGTCAAAG AGGATGCAAA TTCTCTCTAC 
TCAGTAGACC ATGATGGCTA CTACACTTCC ATGCACATGG ACTCTGGAAT CAAATCAAGA 
AATCGGTGCA ATAGCAGTGA TGGCTTTGGG AACCCCAGGC ACAGTGTGAT TAATGTTTTT 
GATGGTAAAG CCCAGAAAAA CCAAGGTGAC CAGTTGCAAA ACTGTGATAA ATCTCTGCCA 
CGAAACATCT CCTTAAAGAA AGCGAAGAAA CCTCCCCTTC CCCCTTTCAG AACAGATTCC 
CTTCGAAGGT CCCCCAAGAA AAAAACTCAC CCCAATGGGC AGGTGCTAAA TGAGACTCTG 
ATTGCTACCC TCCAACATTC CCTACAACTG AATCTGCAGC GGAAAGATAG TAGTTCTCCT 
TCCCAGAGCC CCTGTAGCGA TTACGAAGAT CCATGGCTCC TCCGTTCCCG TAGCCAGAGC 
ACAGTCAGCA CGAGCAGCAG CATGGTGTCG GCCACAGCAC CAAATGTGTA TTCACTTTGC 
GCGGTCACCC CATCTCAGAG CGACACAAGC AGCATCAAGT CAGAGTACGG AGATCCTTGG 
GGTTACTACA TCGACTATAC TGGCGTACAA GAAGATCAGG GGAAATCCAG TGGCACAGGT 
CCACCTAATG CTGTGACTCC ATTGTGTGAT CTTGCCACCA GCCATCTCCA GGATGGATGC 
AGGGTGCCTG TCCCACAAGT GTCTAGTGGA TTGGCCAAAC CAAAGAACGC TTCCCCAGAG 
AAGTCTCACA GGGTCACTTC CCCATCTAGT GGCTATTCCA GCCAATCGAA TACCCCCACA 
GCGCTTACTC CAGTGCCTGT GTTTTTAAAA TCTATCTCAC CAGGAAATGG GAAATCAAAG 
TTGAAGCCCA AAGTGCCTGA AAGAAAATCC TCCCTGATAT CTTCAGTATC TATCTCTTCA 
TCTTCAACTT CCCTTTCTTC CAATACTTCA ACTGAAGGCA GTGTGAATAT GAAGAAATTA 
GATTCAGCTG TAAGCACTCC TCCCATTCCT CCTCTTTCCC CAGCATCTGT ACATCCTCTT 
AATCACTCCC CTCTCCTTCC TCCTCCTCCT TTAGCAGATG CCTTGGAACT GTCCACTGTA 
CAGCATTCTC CCATTTTTCC TCCTCCACCA CCAGAAGTCC TTGCTTCTTC TTTTCTTCCA 
ACGGATACAT GCTTTCCTCT TCCCCCAGCA TCCTTCGGTA TGCCCAATTC AACTGCTCCC 
TTGCCACCTC CTCCTTCATC TCTCTTTCCC TCTTCTGTGC CTCCACCTGC TCCACCTCTT 
GACCCTAAGT TGATGAAAGA AGCTTGGCCC TCTTTAAAAA AGTGTGGCCA ACAGGATTCT 
TCTTGTGCTG TACTTAAACC GTCTCTCACC AAGGAAGAAA CCACTAAACC ACCAATGCCC 
CTAATAACCA CTAAAGCATT ACAGATGGTA CGGTTGAGGC CTGTGAACAA GCCCTCAGAA 
ACATCAGGAG CTCAGCCAGG CAAACCAGCT TCTGAAACAG AATCTCAGGA AAATAATTCT 
GTGGTGCCAC AGGATCCTTT AAAACCATCT TCTGCCTTGA AACACAGAGG GAGCATCAAT 
GGACTAGACA GCAGCAAAGC CCAAAATGTC CCCGTTAAAA ACACACCTCC AACTCCTGAG 
AAGGGCTCGT TTTGGGGTTG GCACAGCAGG ACAGCTGAGC TTGCTGCTCT TCAAAGGCAT 
GGGGATGCAG CTGATCCTGC ACCATCCTCC GAAGCATCCT TTTCAGGAAC AAATGCTGAG 
GAAGGAGGGC AGGTTTCTGG GCAGGACGAG GAATCTCCTT CTGGCCTCAG GACTGCATTG 
CTCCAGGAGC AGCCCAGCTC TCCAAAGAAG ACACATGGCT CTTCTTCACC CAGCAGGAAA 
CCACCTCCTA TTTCAAAGAA ACCCAAACTG TTCCTTGTCG TGCCACCTCC GCACATAGAT 
TTCACAGCAG AGAAAACAAC CAAAGTGAAT GAAAGTGTCA GCAGCCCAGC CAGGGGAGAA 
GTGGTTGATG TGAATGGCAA AGAGGCTCAA GAGAGCTTTG CTGCAGGACC TGGTTCTGCC 
GAGACAGACT TTGGCAGCTT GGTGCCAGGG GGTGGAGCTG TGGGATCCAC CTTCCTTGCA 
GGAGTGGGAG CCGATGTCTA TATGGTACAG CCTGATGTAT CACCAGCTCA AGGAGCTCCC 
AAGCAGGAGG AGCCAACCAC TTCAGATGAA GGCAGCAGGA TAGAGAGCAC TGAAGACAGT 
GGTGGTAACG TGGGCAGTCC CCCCTCTCGA GAGGATGGTA ACATTGGGGT ATTTGAGGCT 
GACATGGTAT GTACCTCTCC TGGACCATGC AGTAGCCTTA AGGAAGATGG GGGTGATGAG 
GTGACAACTC CAAGTAGACC CAGAACTACA GAAGACCTTT TTGCAGCTAT CCACAGGTCT 
AAGAGGAAAG TCCTTGGCCG AAAGGATTCT GATGATGATC ATTCCCGAAA CCATTCTCCA 
TCACCTCCAG TGACACCCAC TGGGGCTGCC CCCAGCCTGG CTTCCCTTAA GCAAGCAGGA 
TCCATCCAGA GAAGCATTCG TAAGAGTAGT ACTAGCAGTG ATAACTTCAA AGCTCTGCTG 
CTGAAAAAGG GGAGCCGGAC AGATGCCAGC TCTCGAATGT CAGCGGCTGA GATGCTGAAG 
AATACAGACC CAAGGTTTCA CAGATCCAAG TCGGAGTCCT CGTCAGACAC CCCTGACAGC 
CCCTCAAGCT GTTCTCCAAA CAAGAACAGG AGGGCCCAGG AGGAGTGGGC CAAGAATGAA 
GGCCTGATGC CAAGGAGTCT GTCCTTCTCT GGCACCAGGT ATGGCCGGAG CCGGACACCG 
CCTTCTGCTG CAAGTAGCAG GTACAGCATG AGGAACCGAA TTCAAAGCAG CCCCATGACT 
GTCATTTCTG AGGGAGAAGG GGAAGCAACA GAGCCCACAG AGAACAGACT CCATCGGACT 
GCAGGAGTTG GAAGGGAAAA AGAAATGGAT GGATTTACTG GGAATGAAAT GGACACTAGT 
GATCTGCCAT ATTCTGAAGA GTGTGGCTCC AAGGGAGCTC AGGCTCCTAA CCATGTTGAC 
TCCACAGATA AGACAGCTAG TAAGACTCTA TCAGGACAAT GTAGAAGTTC TTTAAGGGAA 
GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484690.1
CDS	577..5505
Translation	MVIFINTKIKSLIKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSKSSLACDTYKDEDEESDQKCSISSSEEEKLIATRRARTPTSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQTVQTDVVPINVTGENFDRQASLRRSLIYTDTVVRRPKKVKRRKTITGIPDIIQKELATGTSQNDFRGHSMYVPDHYSTLGRLDNYRPAIQRSETRDSSCQTDEVKVVPPSMRRIRAQKGQGIAAQMSQFSVSSGNMSVMSDSAGVVFPPRFHSDVGFHSLPRPGTRLSLQPLESPQAVENPAEDLAGILAYQMGKLQVDENFEHARCISTPGTLSRPKSEELRHYESEKTSPACIVSPHAAYSTSLIANATISSSSEVIAIHTVQNTGQLDSRIASSSPYARMMKSRDNLATSSAIKDDHRSSSGNWSESSSTRHSQTSDTITSNVVMMASLCDSDASLNTPGNKENGSRNVAYNSRNNLNFLSHSQDSDGKSESSYSGDRAPGGTEPWEYKASGSERASPHKSHCITPGYSTPASNMSSSSLDQASVKEDANSLYSVDHDGYYTSMHMDSGIKSRNRCNSSDGFGNPRHSVINVFDGKAQKNQGDQLQNCDKSLPRNISLKKAKKPPLPPFRTDSLRRSPKKKTHPNGQVLNETLIATLQHSLQLNLQRKDSSSPSQSPCSDYEDPWLLRSRSQSTVSTSSSMVSATAPNVYSLCAVTPSQSDTSSIKSEYGDPWGYYIDYTGVQEDQGKSSGTGPPNAVTPLCDLATSHLQDGCRVPVPQVSSGLAKPKNASPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSISPGNGKSKLKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSVNMKKLDSAVSTPPIPPLSPASVHPLNHSPLLPPPPLADALELSTVQHSPIFPPPPPEVLASSFLPTDTCFPLPPASFGMPNSTAPLPPPPSSLFPSSVPPPAPPLDPKLMKEAWPSLKKCGQQDSSCAVLKPSLTKEETTKPPMPLITTKALQMVRLRPVNKPSETSGAQPGKPASETESQENNSVVPQDPLKPSSALKHRGSINGLDSSKAQNVPVKNTPPTPEKGSFWGWHSRTAELAALQRHGDAADPAPSSEASFSGTNAEEGGQVSGQDEESPSGLRTALLQEQPSSPKKTHGSSSPSRKPPPISKKPKLFLVVPPPHIDFTAEKTTKVNESVSSPARGEVVDVNGKEAQESFAAGPGSAETDFGSLVPGGGAVGSTFLAGVGADVYMVQPDVSPAQGAPKQEEPTTSDEGSRIESTEDSGGNVGSPPSREDGNIGVFEADMVCTSPGPCSSLKEDGGDEVTTPSRPRTTEDLFAAIHRSKRKVLGRKDSDDDHSRNHSPSPPVTPTGAAPSLASLKQAGSIQRSIRKSSTSSDNFKALLLKKGSRTDASSRMSAAEMLKNTDPRFHRSKSESSSDTPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGTRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEATEPTENRLHRTAGVGREKEMDGFTGNEMDTSDLPYSEECGSKGAQAPNHVDSTDKTASKTLSGQCRSSLREES

Target ORF information:

RefSeq Version	XM_007484628.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007484628.2

ORF Insert Sequence:

ATGGTCATAT TCATTAACAC AAAGATCAAG TCTCTGATTA AACTTTTTAA GAAGAAGACT 
GTCTCCAATT TAGATGAAGA GAGTAGATGG ACAGTTCATT ACACAGCACC ATGGCATCAG 
CAGGAGAATG TGTTCCTACC CACCACAAGG CCACCCTGTG TGGAAGACTT GCATCGACAA 
GCCAAGCTGA ACCTCAAATC AGTACTGAGA GAATGTGACA AACTGAGGAG AGATGGTTAT 
CGAAGCTCTC AGTACTACTC TCAGGGACCT ACTTTCTCTA AATCCAGCTT GGCCTGTGAC 
ACCTACAAAG ATGAAGATGA AGAAAGTGAT CAGAAGTGTT CCATCTCTTC CTCAGAGGAA 
GAAAAACTCA TTGCTACCAG GAGAGCCAGA ACTCCCACTT CAAATGATTT ATCTGATATC 
AACACGCAGA CAAATTGGAC CAAGTCACTT CCCCTGCCAA CACCAGAGGA AAAGATGAGA 
CAGCAAGCAC AAACAGTCCA GACAGATGTG GTTCCTATTA ATGTAACTGG GGAGAATTTC 
GACCGCCAAG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTGTCGT AAGACGGCCG 
AAGAAAGTCA AAAGGAGAAA GACTATTACA GGAATCCCTG ACATCATACA AAAGGAGCTA 
GCTACTGGCA CTAGCCAGAA TGACTTCCGT GGTCACTCGA TGTATGTCCC CGACCACTAC 
TCCACACTAG GAAGGCTTGA CAACTATCGA CCCGCCATTC AGCGTTCTGA AACCAGGGAC 
TCTAGTTGTC AGACTGATGA AGTAAAAGTT GTGCCCCCTT CCATGAGAAG AATCAGGGCA 
CAAAAGGGGC AAGGTATTGC TGCCCAGATG AGCCAGTTCT CCGTCTCATC AGGGAACATG 
TCAGTGATGA GCGATTCTGC AGGTGTTGTG TTTCCTCCAC GCTTCCACAG TGATGTGGGT 
TTCCATAGCC TTCCTCGTCC TGGAACAAGG TTGTCCCTTC AACCTCTCGA GTCACCCCAG 
GCTGTTGAGA ACCCTGCAGA GGACTTGGCT GGCATTCTCG CCTACCAGAT GGGTAAGCTG 
CAAGTTGATG AAAACTTTGA GCATGCGAGA TGTATTTCAA CACCTGGGAC ATTATCGAGA 
CCTAAATCAG AAGAGTTGAG ACATTATGAA AGTGAAAAAA CAAGCCCAGC CTGTATTGTT 
TCTCCTCATG CTGCCTACTC TACCAGCCTC ATAGCAAATG CCACAATTTC TTCATCATCG 
GAGGTTATTG CTATACACAC AGTCCAGAAT ACTGGACAGC TGGACAGCAG AATCGCCAGC 
TCATCCCCTT ATGCCAGGAT GATGAAGTCC AGAGACAATC TTGCCACCAG CAGTGCCATA 
AAAGATGATC ATCGGTCTTC CAGTGGTAAC TGGAGCGAGA GTAGCTCGAC ACGACACTCA 
CAGACTTCAG ACACCATTAC CAGCAATGTA GTCATGATGG CGTCCCTCTG TGACTCTGAT 
GCCTCTCTGA ACACTCCAGG AAACAAGGAG AATGGGTCCC GAAATGTAGC CTACAACAGC 
AGAAACAACC TGAATTTCCT AAGCCATTCC CAAGACAGCG ATGGTAAGAG TGAGTCCAGT 
TACTCAGGTG ACAGAGCACC TGGGGGCACA GAGCCCTGGG AATATAAAGC ATCAGGCAGT 
GAGAGGGCAT CCCCACACAA GTCACATTGT ATTACTCCTG GCTACTCCAC TCCTGCTAGT 
AACATGAGCA GCAGTAGTTT AGACCAAGCC TCAGTCAAAG AGGATGCAAA TTCTCTCTAC 
TCAGTAGACC ATGATGGCTA CTACACTTCC ATGCACATGG ACTCTGGAAT CAAATCAAGA 
AATCGGTGCA ATAGCAGTGA TGGCTTTGGG AACCCCAGGC ACAGTGTGAT TAATGTTTTT 
GATGGTAAAG CCCAGAAAAA CCAAGGTGAC CAGTTGCAAA ACTGTGATAA ATCTCTGCCA 
CGAAACATCT CCTTAAAGAA AGCGAAGAAA CCTCCCCTTC CCCCTTTCAG AACAGATTCC 
CTTCGAAGGT CCCCCAAGAA AAAAACTCAC CCCAATGGGC AGGTGCTAAA TGAGACTCTG 
ATTGCTACCC TCCAACATTC CCTACAACTG AATCTGCAGC GGAAAGATAG TAGTTCTCCT 
TCCCAGAGCC CCTGTAGCGA TTACGAAGAT CCATGGCTCC TCCGTTCCCG TAGCCAGAGC 
ACAGTCAGCA CGAGCAGCAG CATGGTGTCG GCCACAGCAC CAAATGTGTA TTCACTTTGC 
GCGGTCACCC CATCTCAGAG CGACACAAGC AGCATCAAGT CAGAGTACGG AGATCCTTGG 
GGTTACTACA TCGACTATAC TGGCGTACAA GAAGATCAGG GGAAATCCAG TGGCACAGGT 
CCACCTAATG CTGTGACTCC ATTGTGTGAT CTTGCCACCA GCCATCTCCA GGATGGATGC 
AGGGTGCCTG TCCCACAAGT GTCTAGTGGA TTGGCCAAAC CAAAGAACGC TTCCCCAGAG 
AAGTCTCACA GGGTCACTTC CCCATCTAGT GGCTATTCCA GCCAATCGAA TACCCCCACA 
GCGCTTACTC CAGTGCCTGT GTTTTTAAAA TCTATCTCAC CAGGAAATGG GAAATCAAAG 
TTGAAGCCCA AAGTGCCTGA AAGAAAATCC TCCCTGATAT CTTCAGTATC TATCTCTTCA 
TCTTCAACTT CCCTTTCTTC CAATACTTCA ACTGAAGGCA GTGTGAATAT GAAGAAATTA 
GATTCAGCTG TAAGCACTCC TCCCATTCCT CCTCTTTCCC CAGCATCTGT ACATCCTCTT 
AATCACTCCC CTCTCCTTCC TCCTCCTCCT TTAGCAGATG CCTTGGAACT GTCCACTGTA 
CAGCATTCTC CCATTTTTCC TCCTCCACCA CCAGAAGTCC TTGCTTCTTC TTTTCTTCCA 
ACGGATACAT GCTTTCCTCT TCCCCCAGCA TCCTTCGGTA TGCCCAATTC AACTGCTCCC 
TTGCCACCTC CTCCTTCATC TCTCTTTCCC TCTTCTGTGC CTCCACCTGC TCCACCTCTT 
GACCCTAAGT TGATGAAAGA AGCTTGGCCC TCTTTAAAAA AGTGTGGCCA ACAGGATTCT 
TCTTGTGCTG TACTTAAACC GTCTCTCACC AAGGAAGAAA CCACTAAACC ACCAATGCCC 
CTAATAACCA CTAAAGCATT ACAGATGGTA CGGTTGAGGC CTGTGAACAA GCCCTCAGAA 
ACATCAGGAG CTCAGCCAGG CAAACCAGCT TCTGAAACAG AATCTCAGGA AAATAATTCT 
GTGGTGCCAC AGGATCCTTT AAAACCATCT TCTGCCTTGA AACACAGAGG GAGCATCAAT 
GGACTAGACA GCAGCAAAGC CCAAAATGTC CCCGTTAAAA ACACACCTCC AACTCCTGAG 
AAGGGCTCGT TTTGGGGTTG GCACAGCAGG ACAGCTGAGC TTGCTGCTCT TCAAAGGCAT 
GGGGATGCAG CTGATCCTGC ACCATCCTCC GAAGCATCCT TTTCAGGAAC AAATGCTGAG 
GAAGGAGGGC AGGTTTCTGG GCAGGACGAG GAATCTCCTT CTGGCCTCAG GACTGCATTG 
CTCCAGGAGC AGCCCAGCTC TCCAAAGAAG ACACATGGCT CTTCTTCACC CAGCAGGAAA 
CCACCTCCTA TTTCAAAGAA ACCCAAACTG TTCCTTGTCG TGCCACCTCC GCACATAGAT 
TTCACAGCAG AGAAAACAAC CAAAGTGAAT GAAAGTGTCA GCAGCCCAGC CAGGGGAGAA 
GTGGTTGATG TGAATGGCAA AGAGGCTCAA GAGAGCTTTG CTGCAGGACC TGGTTCTGCC 
GAGACAGACT TTGGCAGCTT GGTGCCAGGG GGTGGAGCTG TGGGATCCAC CTTCCTTGCA 
GGAGTGGGAG CCGATGTCTA TATGGTACAG CCTGATGTAT CACCAGCTCA AGGAGCTCCC 
AAGCAGGAGG AGCCAACCAC TTCAGATGAA GGCAGCAGGA TAGAGAGCAC TGAAGACAGT 
GGTGGTAACG TGGGCAGTCC CCCCTCTCGA GAGGATGGTA ACATTGGGGT ATTTGAGGCT 
GACATGGTAT GTACCTCTCC TGGACCATGC AGTAGCCTTA AGGAAGATGG GGGTGATGAG 
GTGACAACTC CAAGTAGACC CAGAACTACA GAAGACCTTT TTGCAGCTAT CCACAGGTCT 
AAGAGGAAAG TCCTTGGCCG AAAGGATTCT GATGATGATC ATTCCCGAAA CCATTCTCCA 
TCACCTCCAG TGACACCCAC TGGGGCTGCC CCCAGCCTGG CTTCCCTTAA GCAAGCAGGA 
TCCATCCAGA GAAGCATTCG TAAGAGTAGT ACTAGCAGTG ATAACTTCAA AGCTCTGCTG 
CTGAAAAAGG GGAGCCGGAC AGATGCCAGC TCTCGAATGT CAGCGGCTGA GATGCTGAAG 
AATACAGACC CAAGGTTTCA CAGATCCAAG TCGGAGTCCT CGTCAGACAC CCCTGACAGC 
CCCTCAAGCT GTTCTCCAAA CAAGAACAGG AGGGCCCAGG AGGAGTGGGC CAAGAATGAA 
GGCCTGATGC CAAGGAGTCT GTCCTTCTCT GGCACCAGGT ATGGCCGGAG CCGGACACCG 
CCTTCTGCTG CAAGTAGCAG GTACAGCATG AGGAACCGAA TTCAAAGCAG CCCCATGACT 
GTCATTTCTG AGGGAGAAGG GGAAGCAACA GAGCCCACAG AGAACAGACT CCATCGGACT 
GCAGGAGTTG GAAGGGAAAA AGAAATGGAT GGATTTACTG GGAATGAAAT GGACACTAGT 
GATCTGCCAT ATTCTGAAGA GTGTGGCTCC AAGGGAGCTC AGGCTCCTAA CCATGTTGAC 
TCCACAGATA AGACAGCTAG TAAGACTCTA TCAGGACAAT GTAGAAGTTC TTTAAGGGAA 
GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_007484629.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484691.1 Monodelphis domestica NHS like 1 (NHSL1), transcript variant X6, mRNA.

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CloneID	OMc12988
Clone ID Related Accession (Same CDS sequence)	XM_007484629.2
Accession Version	XM_007484629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007484629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGTC TCAAAGCTGT CTCCAATTTA GATGAAGAGA GTAGATGGAC AGTTCATTAC 
ACAGCACCAT GGCATCAGCA GGAGAATGTG TTCCTACCCA CCACAAGGCC ACCCTGTGTG 
GAAGACTTGC ATCGACAAGC CAAGCTGAAC CTCAAATCAG TACTGAGAGA ATGTGACAAA 
CTGAGGAGAG ATGGTTATCG AAGCTCTCAG TACTACTCTC AGGGACCTAC TTTCTCTAAA 
TCCAGCTTGG CCTGTGACAC CTACAAAGAT GAAGATGAAG AAAGTGATCA GAAGTGTTCC 
ATCTCTTCCT CAGAGGAAGA AAAACTCATT GCTACCAGGA GAGCCAGAAC TCCCACTTCA 
AATGATTTAT CTGATATCAA CACGCAGACA AATTGGACCA AGTCACTTCC CCTGCCAACA 
CCAGAGGAAA AGATGAGACA GCAAGCACAA ACAGTCCAGA CAGATGTGGT TCCTATTAAT 
GTAACTGGGG AGAATTTCGA CCGCCAAGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC 
ACTGTCGTAA GACGGCCGAA GAAAGTCAAA AGGAGAAAGA CTATTACAGG AATCCCTGAC 
ATCATACAAA AGGAGCTAGC TACTGGCACT AGCCAGAATG ACTTCCGTGG TCACTCGATG 
TATGTCCCCG ACCACTACTC CACACTAGGA AGGCTTGACA ACTATCGACC CGCCATTCAG 
CGTTCTGAAA CCAGGGACTC TAGTTGTCAG ACTGATGAAG TAAAAGTTGT GCCCCCTTCC 
ATGAGAAGAA TCAGGGCACA AAAGGGGCAA GGTATTGCTG CCCAGATGAG CCAGTTCTCC 
GTCTCATCAG GGAACATGTC AGTGATGAGC GATTCTGCAG GTGTTGTGTT TCCTCCACGC 
TTCCACAGTG ATGTGGGTTT CCATAGCCTT CCTCGTCCTG GAACAAGGTT GTCCCTTCAA 
CCTCTCGAGT CACCCCAGGC TGTTGAGAAC CCTGCAGAGG ACTTGGCTGG CATTCTCGCC 
TACCAGATGG GTAAGCTGCA AGTTGATGAA AACTTTGAGC ATGCGAGATG TATTTCAACA 
CCTGGGACAT TATCGAGACC TAAATCAGAA GAGTTGAGAC ATTATGAAAG TGAAAAAACA 
AGCCCAGCCT GTATTGTTTC TCCTCATGCT GCCTACTCTA CCAGCCTCAT AGCAAATGCC 
ACAATTTCTT CATCATCGGA GGTTATTGCT ATACACACAG TCCAGAATAC TGGACAGCTG 
GACAGCAGAA TCGCCAGCTC ATCCCCTTAT GCCAGGATGA TGAAGTCCAG AGACAATCTT 
GCCACCAGCA GTGCCATAAA AGATGATCAT CGGTCTTCCA GTGGTAACTG GAGCGAGAGT 
AGCTCGACAC GACACTCACA GACTTCAGAC ACCATTACCA GCAATGTAGT CATGATGGCG 
TCCCTCTGTG ACTCTGATGC CTCTCTGAAC ACTCCAGGAA ACAAGGAGAA TGGGTCCCGA 
AATGTAGCCT ACAACAGCAG AAACAACCTG AATTTCCTAA GCCATTCCCA AGACAGCGAT 
GGTAAGAGTG AGTCCAGTTA CTCAGGTGAC AGAGCACCTG GGGGCACAGA GCCCTGGGAA 
TATAAAGCAT CAGGCAGTGA GAGGGCATCC CCACACAAGT CACATTGTAT TACTCCTGGC 
TACTCCACTC CTGCTAGTAA CATGAGCAGC AGTAGTTTAG ACCAAGCCTC AGTCAAAGAG 
GATGCAAATT CTCTCTACTC AGTAGACCAT GATGGCTACT ACACTTCCAT GCACATGGAC 
TCTGGAATCA AATCAAGAAA TCGGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGTGTGATTA ATGTTTTTGA TGGTAAAGCC CAGAAAAACC AAGGTGACCA GTTGCAAAAC 
TGTGATAAAT CTCTGCCACG AAACATCTCC TTAAAGAAAG CGAAGAAACC TCCCCTTCCC 
CCTTTCAGAA CAGATTCCCT TCGAAGGTCC CCCAAGAAAA AAACTCACCC CAATGGGCAG 
GTGCTAAATG AGACTCTGAT TGCTACCCTC CAACATTCCC TACAACTGAA TCTGCAGCGG 
AAAGATAGTA GTTCTCCTTC CCAGAGCCCC TGTAGCGATT ACGAAGATCC ATGGCTCCTC 
CGTTCCCGTA GCCAGAGCAC AGTCAGCACG AGCAGCAGCA TGGTGTCGGC CACAGCACCA 
AATGTGTATT CACTTTGCGC GGTCACCCCA TCTCAGAGCG ACACAAGCAG CATCAAGTCA 
GAGTACGGAG ATCCTTGGGG TTACTACATC GACTATACTG GCGTACAAGA AGATCAGGGG 
AAATCCAGTG GCACAGGTCC ACCTAATGCT GTGACTCCAT TGTGTGATCT TGCCACCAGC 
CATCTCCAGG ATGGATGCAG GGTGCCTGTC CCACAAGTGT CTAGTGGATT GGCCAAACCA 
AAGAACGCTT CCCCAGAGAA GTCTCACAGG GTCACTTCCC CATCTAGTGG CTATTCCAGC 
CAATCGAATA CCCCCACAGC GCTTACTCCA GTGCCTGTGT TTTTAAAATC TATCTCACCA 
GGAAATGGGA AATCAAAGTT GAAGCCCAAA GTGCCTGAAA GAAAATCCTC CCTGATATCT 
TCAGTATCTA TCTCTTCATC TTCAACTTCC CTTTCTTCCA ATACTTCAAC TGAAGGCAGT 
GTGAATATGA AGAAATTAGA TTCAGCTGTA AGCACTCCTC CCATTCCTCC TCTTTCCCCA 
GCATCTGTAC ATCCTCTTAA TCACTCCCCT CTCCTTCCTC CTCCTCCTTT AGCAGATGCC 
TTGGAACTGT CCACTGTACA GCATTCTCCC ATTTTTCCTC CTCCACCACC AGAAGTCCTT 
GCTTCTTCTT TTCTTCCAAC GGATACATGC TTTCCTCTTC CCCCAGCATC CTTCGGTATG 
CCCAATTCAA CTGCTCCCTT GCCACCTCCT CCTTCATCTC TCTTTCCCTC TTCTGTGCCT 
CCACCTGCTC CACCTCTTGA CCCTAAGTTG ATGAAAGAAG CTTGGCCCTC TTTAAAAAAG 
TGTGGCCAAC AGGATTCTTC TTGTGCTGTA CTTAAACCGT CTCTCACCAA GGAAGAAACC 
ACTAAACCAC CAATGCCCCT AATAACCACT AAAGCATTAC AGATGGTACG GTTGAGGCCT 
GTGAACAAGC CCTCAGAAAC ATCAGGAGCT CAGCCAGGCA AACCAGCTTC TGAAACAGAA 
TCTCAGGAAA ATAATTCTGT GGTGCCACAG GATCCTTTAA AACCATCTTC TGCCTTGAAA 
CACAGAGGGA GCATCAATGG ACTAGACAGC AGCAAAGCCC AAAATGTCCC CGTTAAAAAC 
ACACCTCCAA CTCCTGAGAA GGGCTCGTTT TGGGGTTGGC ACAGCAGGAC AGCTGAGCTT 
GCTGCTCTTC AAAGGCATGG GGATGCAGCT GATCCTGCAC CATCCTCCGA AGCATCCTTT 
TCAGGAACAA ATGCTGAGGA AGGAGGGCAG GTTTCTGGGC AGGACGAGGA ATCTCCTTCT 
GGCCTCAGGA CTGCATTGCT CCAGGAGCAG CCCAGCTCTC CAAAGAAGAC ACATGGCTCT 
TCTTCACCCA GCAGGAAACC ACCTCCTATT TCAAAGAAAC CCAAACTGTT CCTTGTCGTG 
CCACCTCCGC ACATAGATTT CACAGCAGAG AAAACAACCA AAGTGAATGA AAGTGTCAGC 
AGCCCAGCCA GGGGAGAAGT GGTTGATGTG AATGGCAAAG AGGCTCAAGA GAGCTTTGCT 
GCAGGACCTG GTTCTGCCGA GACAGACTTT GGCAGCTTGG TGCCAGGGGG TGGAGCTGTG 
GGATCCACCT TCCTTGCAGG AGTGGGAGCC GATGTCTATA TGGTACAGCC TGATGTATCA 
CCAGCTCAAG GAGCTCCCAA GCAGGAGGAG CCAACCACTT CAGATGAAGG CAGCAGGATA 
GAGAGCACTG AAGACAGTGG TGGTAACGTG GGCAGTCCCC CCTCTCGAGA GGATGGTAAC 
ATTGGGGTAT TTGAGGCTGA CATGGTATGT ACCTCTCCTG GACCATGCAG TAGCCTTAAG 
GAAGATGGGG GTGATGAGGT GACAACTCCA AGTAGACCCA GAACTACAGA AGACCTTTTT 
GCAGCTATCC ACAGGTCTAA GAGGAAAGTC CTTGGCCGAA AGGATTCTGA TGATGATCAT 
TCCCGAAACC ATTCTCCATC ACCTCCAGTG ACACCCACTG GGGCTGCCCC CAGCCTGGCT 
TCCCTTAAGC AAGCAGGATC CATCCAGAGA AGCATTCGTA AGAGTAGTAC TAGCAGTGAT 
AACTTCAAAG CTCTGCTGCT GAAAAAGGGG AGCCGGACAG ATGCCAGCTC TCGAATGTCA 
GCGGCTGAGA TGCTGAAGAA TACAGACCCA AGGTTTCACA GATCCAAGTC GGAGTCCTCG 
TCAGACACCC CTGACAGCCC CTCAAGCTGT TCTCCAAACA AGAACAGGAG GGCCCAGGAG 
GAGTGGGCCA AGAATGAAGG CCTGATGCCA AGGAGTCTGT CCTTCTCTGG CACCAGGTAT 
GGCCGGAGCC GGACACCGCC TTCTGCTGCA AGTAGCAGGT ACAGCATGAG GAACCGAATT 
CAAAGCAGCC CCATGACTGT CATTTCTGAG GGAGAAGGGG AAGCAACAGA GCCCACAGAG 
AACAGACTCC ATCGGACTGC AGGAGTTGGA AGGGAAAAAG AAATGGATGG ATTTACTGGG 
AATGAAATGG ACACTAGTGA TCTGCCATAT TCTGAAGAGT GTGGCTCCAA GGGAGCTCAG 
GCTCCTAACC ATGTTGACTC CACAGATAAG ACAGCTAGTA AGACTCTATC AGGACAATGT 
AGAAGTTCTT TAAGGGAAGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484691.1
CDS	111..4997
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSKSSLACDTYKDEDEESDQKCSISSSEEEKLIATRRARTPTSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQTVQTDVVPINVTGENFDRQASLRRSLIYTDTVVRRPKKVKRRKTITGIPDIIQKELATGTSQNDFRGHSMYVPDHYSTLGRLDNYRPAIQRSETRDSSCQTDEVKVVPPSMRRIRAQKGQGIAAQMSQFSVSSGNMSVMSDSAGVVFPPRFHSDVGFHSLPRPGTRLSLQPLESPQAVENPAEDLAGILAYQMGKLQVDENFEHARCISTPGTLSRPKSEELRHYESEKTSPACIVSPHAAYSTSLIANATISSSSEVIAIHTVQNTGQLDSRIASSSPYARMMKSRDNLATSSAIKDDHRSSSGNWSESSSTRHSQTSDTITSNVVMMASLCDSDASLNTPGNKENGSRNVAYNSRNNLNFLSHSQDSDGKSESSYSGDRAPGGTEPWEYKASGSERASPHKSHCITPGYSTPASNMSSSSLDQASVKEDANSLYSVDHDGYYTSMHMDSGIKSRNRCNSSDGFGNPRHSVINVFDGKAQKNQGDQLQNCDKSLPRNISLKKAKKPPLPPFRTDSLRRSPKKKTHPNGQVLNETLIATLQHSLQLNLQRKDSSSPSQSPCSDYEDPWLLRSRSQSTVSTSSSMVSATAPNVYSLCAVTPSQSDTSSIKSEYGDPWGYYIDYTGVQEDQGKSSGTGPPNAVTPLCDLATSHLQDGCRVPVPQVSSGLAKPKNASPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSISPGNGKSKLKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSVNMKKLDSAVSTPPIPPLSPASVHPLNHSPLLPPPPLADALELSTVQHSPIFPPPPPEVLASSFLPTDTCFPLPPASFGMPNSTAPLPPPPSSLFPSSVPPPAPPLDPKLMKEAWPSLKKCGQQDSSCAVLKPSLTKEETTKPPMPLITTKALQMVRLRPVNKPSETSGAQPGKPASETESQENNSVVPQDPLKPSSALKHRGSINGLDSSKAQNVPVKNTPPTPEKGSFWGWHSRTAELAALQRHGDAADPAPSSEASFSGTNAEEGGQVSGQDEESPSGLRTALLQEQPSSPKKTHGSSSPSRKPPPISKKPKLFLVVPPPHIDFTAEKTTKVNESVSSPARGEVVDVNGKEAQESFAAGPGSAETDFGSLVPGGGAVGSTFLAGVGADVYMVQPDVSPAQGAPKQEEPTTSDEGSRIESTEDSGGNVGSPPSREDGNIGVFEADMVCTSPGPCSSLKEDGGDEVTTPSRPRTTEDLFAAIHRSKRKVLGRKDSDDDHSRNHSPSPPVTPTGAAPSLASLKQAGSIQRSIRKSSTSSDNFKALLLKKGSRTDASSRMSAAEMLKNTDPRFHRSKSESSSDTPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLSFSGTRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEATEPTENRLHRTAGVGREKEMDGFTGNEMDTSDLPYSEECGSKGAQAPNHVDSTDKTASKTLSGQCRSSLREES

Target ORF information:

RefSeq Version	XM_007484629.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NHS like 1 (NHSL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007484629.2

ORF Insert Sequence:

ATGTTCTGTC TCAAAGCTGT CTCCAATTTA GATGAAGAGA GTAGATGGAC AGTTCATTAC 
ACAGCACCAT GGCATCAGCA GGAGAATGTG TTCCTACCCA CCACAAGGCC ACCCTGTGTG 
GAAGACTTGC ATCGACAAGC CAAGCTGAAC CTCAAATCAG TACTGAGAGA ATGTGACAAA 
CTGAGGAGAG ATGGTTATCG AAGCTCTCAG TACTACTCTC AGGGACCTAC TTTCTCTAAA 
TCCAGCTTGG CCTGTGACAC CTACAAAGAT GAAGATGAAG AAAGTGATCA GAAGTGTTCC 
ATCTCTTCCT CAGAGGAAGA AAAACTCATT GCTACCAGGA GAGCCAGAAC TCCCACTTCA 
AATGATTTAT CTGATATCAA CACGCAGACA AATTGGACCA AGTCACTTCC CCTGCCAACA 
CCAGAGGAAA AGATGAGACA GCAAGCACAA ACAGTCCAGA CAGATGTGGT TCCTATTAAT 
GTAACTGGGG AGAATTTCGA CCGCCAAGCC AGCCTTCGGC GGTCTCTAAT TTACACAGAC 
ACTGTCGTAA GACGGCCGAA GAAAGTCAAA AGGAGAAAGA CTATTACAGG AATCCCTGAC 
ATCATACAAA AGGAGCTAGC TACTGGCACT AGCCAGAATG ACTTCCGTGG TCACTCGATG 
TATGTCCCCG ACCACTACTC CACACTAGGA AGGCTTGACA ACTATCGACC CGCCATTCAG 
CGTTCTGAAA CCAGGGACTC TAGTTGTCAG ACTGATGAAG TAAAAGTTGT GCCCCCTTCC 
ATGAGAAGAA TCAGGGCACA AAAGGGGCAA GGTATTGCTG CCCAGATGAG CCAGTTCTCC 
GTCTCATCAG GGAACATGTC AGTGATGAGC GATTCTGCAG GTGTTGTGTT TCCTCCACGC 
TTCCACAGTG ATGTGGGTTT CCATAGCCTT CCTCGTCCTG GAACAAGGTT GTCCCTTCAA 
CCTCTCGAGT CACCCCAGGC TGTTGAGAAC CCTGCAGAGG ACTTGGCTGG CATTCTCGCC 
TACCAGATGG GTAAGCTGCA AGTTGATGAA AACTTTGAGC ATGCGAGATG TATTTCAACA 
CCTGGGACAT TATCGAGACC TAAATCAGAA GAGTTGAGAC ATTATGAAAG TGAAAAAACA 
AGCCCAGCCT GTATTGTTTC TCCTCATGCT GCCTACTCTA CCAGCCTCAT AGCAAATGCC 
ACAATTTCTT CATCATCGGA GGTTATTGCT ATACACACAG TCCAGAATAC TGGACAGCTG 
GACAGCAGAA TCGCCAGCTC ATCCCCTTAT GCCAGGATGA TGAAGTCCAG AGACAATCTT 
GCCACCAGCA GTGCCATAAA AGATGATCAT CGGTCTTCCA GTGGTAACTG GAGCGAGAGT 
AGCTCGACAC GACACTCACA GACTTCAGAC ACCATTACCA GCAATGTAGT CATGATGGCG 
TCCCTCTGTG ACTCTGATGC CTCTCTGAAC ACTCCAGGAA ACAAGGAGAA TGGGTCCCGA 
AATGTAGCCT ACAACAGCAG AAACAACCTG AATTTCCTAA GCCATTCCCA AGACAGCGAT 
GGTAAGAGTG AGTCCAGTTA CTCAGGTGAC AGAGCACCTG GGGGCACAGA GCCCTGGGAA 
TATAAAGCAT CAGGCAGTGA GAGGGCATCC CCACACAAGT CACATTGTAT TACTCCTGGC 
TACTCCACTC CTGCTAGTAA CATGAGCAGC AGTAGTTTAG ACCAAGCCTC AGTCAAAGAG 
GATGCAAATT CTCTCTACTC AGTAGACCAT GATGGCTACT ACACTTCCAT GCACATGGAC 
TCTGGAATCA AATCAAGAAA TCGGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGTGTGATTA ATGTTTTTGA TGGTAAAGCC CAGAAAAACC AAGGTGACCA GTTGCAAAAC 
TGTGATAAAT CTCTGCCACG AAACATCTCC TTAAAGAAAG CGAAGAAACC TCCCCTTCCC 
CCTTTCAGAA CAGATTCCCT TCGAAGGTCC CCCAAGAAAA AAACTCACCC CAATGGGCAG 
GTGCTAAATG AGACTCTGAT TGCTACCCTC CAACATTCCC TACAACTGAA TCTGCAGCGG 
AAAGATAGTA GTTCTCCTTC CCAGAGCCCC TGTAGCGATT ACGAAGATCC ATGGCTCCTC 
CGTTCCCGTA GCCAGAGCAC AGTCAGCACG AGCAGCAGCA TGGTGTCGGC CACAGCACCA 
AATGTGTATT CACTTTGCGC GGTCACCCCA TCTCAGAGCG ACACAAGCAG CATCAAGTCA 
GAGTACGGAG ATCCTTGGGG TTACTACATC GACTATACTG GCGTACAAGA AGATCAGGGG 
AAATCCAGTG GCACAGGTCC ACCTAATGCT GTGACTCCAT TGTGTGATCT TGCCACCAGC 
CATCTCCAGG ATGGATGCAG GGTGCCTGTC CCACAAGTGT CTAGTGGATT GGCCAAACCA 
AAGAACGCTT CCCCAGAGAA GTCTCACAGG GTCACTTCCC CATCTAGTGG CTATTCCAGC 
CAATCGAATA CCCCCACAGC GCTTACTCCA GTGCCTGTGT TTTTAAAATC TATCTCACCA 
GGAAATGGGA AATCAAAGTT GAAGCCCAAA GTGCCTGAAA GAAAATCCTC CCTGATATCT 
TCAGTATCTA TCTCTTCATC TTCAACTTCC CTTTCTTCCA ATACTTCAAC TGAAGGCAGT 
GTGAATATGA AGAAATTAGA TTCAGCTGTA AGCACTCCTC CCATTCCTCC TCTTTCCCCA 
GCATCTGTAC ATCCTCTTAA TCACTCCCCT CTCCTTCCTC CTCCTCCTTT AGCAGATGCC 
TTGGAACTGT CCACTGTACA GCATTCTCCC ATTTTTCCTC CTCCACCACC AGAAGTCCTT 
GCTTCTTCTT TTCTTCCAAC GGATACATGC TTTCCTCTTC CCCCAGCATC CTTCGGTATG 
CCCAATTCAA CTGCTCCCTT GCCACCTCCT CCTTCATCTC TCTTTCCCTC TTCTGTGCCT 
CCACCTGCTC CACCTCTTGA CCCTAAGTTG ATGAAAGAAG CTTGGCCCTC TTTAAAAAAG 
TGTGGCCAAC AGGATTCTTC TTGTGCTGTA CTTAAACCGT CTCTCACCAA GGAAGAAACC 
ACTAAACCAC CAATGCCCCT AATAACCACT AAAGCATTAC AGATGGTACG GTTGAGGCCT 
GTGAACAAGC CCTCAGAAAC ATCAGGAGCT CAGCCAGGCA AACCAGCTTC TGAAACAGAA 
TCTCAGGAAA ATAATTCTGT GGTGCCACAG GATCCTTTAA AACCATCTTC TGCCTTGAAA 
CACAGAGGGA GCATCAATGG ACTAGACAGC AGCAAAGCCC AAAATGTCCC CGTTAAAAAC 
ACACCTCCAA CTCCTGAGAA GGGCTCGTTT TGGGGTTGGC ACAGCAGGAC AGCTGAGCTT 
GCTGCTCTTC AAAGGCATGG GGATGCAGCT GATCCTGCAC CATCCTCCGA AGCATCCTTT 
TCAGGAACAA ATGCTGAGGA AGGAGGGCAG GTTTCTGGGC AGGACGAGGA ATCTCCTTCT 
GGCCTCAGGA CTGCATTGCT CCAGGAGCAG CCCAGCTCTC CAAAGAAGAC ACATGGCTCT 
TCTTCACCCA GCAGGAAACC ACCTCCTATT TCAAAGAAAC CCAAACTGTT CCTTGTCGTG 
CCACCTCCGC ACATAGATTT CACAGCAGAG AAAACAACCA AAGTGAATGA AAGTGTCAGC 
AGCCCAGCCA GGGGAGAAGT GGTTGATGTG AATGGCAAAG AGGCTCAAGA GAGCTTTGCT 
GCAGGACCTG GTTCTGCCGA GACAGACTTT GGCAGCTTGG TGCCAGGGGG TGGAGCTGTG 
GGATCCACCT TCCTTGCAGG AGTGGGAGCC GATGTCTATA TGGTACAGCC TGATGTATCA 
CCAGCTCAAG GAGCTCCCAA GCAGGAGGAG CCAACCACTT CAGATGAAGG CAGCAGGATA 
GAGAGCACTG AAGACAGTGG TGGTAACGTG GGCAGTCCCC CCTCTCGAGA GGATGGTAAC 
ATTGGGGTAT TTGAGGCTGA CATGGTATGT ACCTCTCCTG GACCATGCAG TAGCCTTAAG 
GAAGATGGGG GTGATGAGGT GACAACTCCA AGTAGACCCA GAACTACAGA AGACCTTTTT 
GCAGCTATCC ACAGGTCTAA GAGGAAAGTC CTTGGCCGAA AGGATTCTGA TGATGATCAT 
TCCCGAAACC ATTCTCCATC ACCTCCAGTG ACACCCACTG GGGCTGCCCC CAGCCTGGCT 
TCCCTTAAGC AAGCAGGATC CATCCAGAGA AGCATTCGTA AGAGTAGTAC TAGCAGTGAT 
AACTTCAAAG CTCTGCTGCT GAAAAAGGGG AGCCGGACAG ATGCCAGCTC TCGAATGTCA 
GCGGCTGAGA TGCTGAAGAA TACAGACCCA AGGTTTCACA GATCCAAGTC GGAGTCCTCG 
TCAGACACCC CTGACAGCCC CTCAAGCTGT TCTCCAAACA AGAACAGGAG GGCCCAGGAG 
GAGTGGGCCA AGAATGAAGG CCTGATGCCA AGGAGTCTGT CCTTCTCTGG CACCAGGTAT 
GGCCGGAGCC GGACACCGCC TTCTGCTGCA AGTAGCAGGT ACAGCATGAG GAACCGAATT 
CAAAGCAGCC CCATGACTGT CATTTCTGAG GGAGAAGGGG AAGCAACAGA GCCCACAGAG 
AACAGACTCC ATCGGACTGC AGGAGTTGGA AGGGAAAAAG AAATGGATGG ATTTACTGGG 
AATGAAATGG ACACTAGTGA TCTGCCATAT TCTGAAGAGT GTGGCTCCAA GGGAGCTCAG 
GCTCCTAACC ATGTTGACTC CACAGATAAG ACAGCTAGTA AGACTCTATC AGGACAATGT 
AGAAGTTCTT TAAGGGAAGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NHSL1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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