LINJ_20_0700 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_20_0700
Entrez Gene ID	5068604
Full Name	putative kinesin heavy chain
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001465152.1	XP_001465189.1	putative kinesin heavy chain

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_20_0700 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_20_0700 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc02327	XM_001465152.1 Latest version!	Leishmania infantum JPCM5 putative kinesin heavy chain partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_20_0700 ( XM_001465152.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001465189.1 Leishmania infantum JPCM5 putative kinesin heavy chain partial mRNA.

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CloneID	OLc02327
Clone ID Related Accession (Same CDS sequence)	XM_001465152.1
Accession Version	XM_001465152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative kinesin heavy chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009404). COMPLETENESS: incomplete on both ends.

ATGTATGGGC AGACTGGGAG CGGCAAGACG CACACGATGT TCGGAGAAGT GAATGGTGGT 
GCATGGGCCA CAAGCTGCGA CTCAGGCAGG CCCTCTGCAG GCGTCGCCTT CAACGCAGAC 
ACCAACGAGG AGGACGTCAA CGAGAAGCCG AAGGAGGAGG ACAGTCCTGC GCACACGCGC 
CTCACCGAAG AGGAGCACGG AGAGATGGAG CCTCACGCTT GCGAGCGTCA GAGAGCACAA 
GCGGAAGGCG AAGACGAGGA ACGACACGAA GAGCAGAAGC AGCAGCAAGC ATCTCAGCAA 
ACCTTGCAGG CGCCCGGCAC GTCTCCCAGG GCAACGCGGC CGAAACTCAG CACCTACCAT 
ACCAAGACGG GCCACCCGGC GACCTCAGCG ATAGCGACGA CAAGGAGCGC CGCAGCCGTG 
CCACCACCGT CCGCGACGAC GACATCTTTC TGCCGCACCC GCGCCACCGT CACTCCGCTT 
GGCGAGCTGC AGCAAATCAA CACGCTTCAA AACGCTACCG GCAGTGACGC TGCGGAGTAC 
TGCTATGCAG CGGTGAACAA GTCGACAAAA ATGTGTGCCG CCGCCACCGC GTCGACCTCT 
TACGCTCCAC CGTCGTCGTC AGCGGCCCCA GCATCGCCGT CCTCTGCGTT CTCCCGCAGT 
GGACCGCCTC GGTGTACGGT GAGGAGTGCG GCGGGTATGC GGTCCGCCTT TACGGGCGAC 
GCCGTCAGCG GCGTCATACC GCGAGCGGTG CACGACATCT TCGACGCCAT CGCCCACGCA 
GACCCGTCCA AGGAGTTCGA CGTGCAGATG TTCTTTGTGG AGGTGTACAT GGAGCAGATT 
CGCGACCTCC TTGTCCCGAA CCCGTCCGCC GCGATGCATT CATCGTCGGC TGGTATCGCT 
CCAACGCTGC CGATAGGGCC ACGCAAGCTG CAGCTGCGCG AGGATGTTAG CACCAACTCG 
TTCTACGTCG ATGGATGTTG CAACCCGCAT GTGTCGTCTG CCAGGGATGT GCTGCATCTG 
GTGAAGAACG GTCTCAAGCA GCGGGCCACC TCAGCCACGG CGATGAACGA GACGAGCAGC 
CGCAGCCATT GCCTGCTGAA CCTGACGGTG AAGAGCGTCG ACCGCGCGCA GGGGGTGTCG 
ACGGTAGGGA AGCTTTACTT GGTGGACCTC GCTGGTAGCG AGAAGGTTGG GAAAACCAAC 
GCGACAGGGC TGCGCCTAGA GGAGGCAAAG CTGATCAACA AGTCCCTCTC CACTCTTGGC 
ATGGTTATCA TGTCCCTGAC AGATCACAGC GCCACGCACA CGCCGTATCG AGATAGTGTG 
CTGACAAAAA TCTTGAAAGA CTCGCTCGGC GGCAACTCGC ACACGGCCCT TGTGATCTGC 
TGCAGCCCGT CCCCGTGCCA TGCGCAGGAG ACGCTGTCGA CGCTGCGCTT CGGTGCTCGC 
GCGAAGGCTA TCGAGAACAA GGCCGTCGTG AACCGCGACC TCACCGCGGC GCAGCTGCGG 
CGCATGTTGG AGACGGCCAA GGGGGAGATC GAGCGGCTGC ACGTGAAGGT GCAGCAGCTG 
AGCATGACGA GTGGAGCAGT GGACCCCTGT AAAGGGACGC CGGACTTCCC CGTCGTAGCA 
TCGCGAAAGC TGTCGAGCGC CGCCACTGCT ACGGCGTCTT CAATGGCGAG CGAGGCGGAC 
ACGTCCCGCA TCAGTGCACT GCTGGAGGAG CGTGCGACGG AGCAGGCGCG GTTGCAGAAT 
CTCCGCGCTG AGGTGGCGCA GCTGCATGAC AGCCTCAGCA GCGCCCAGGA CATCATCAGT 
GCCTTGCAGG AGGAGCGCGA CGGCTACATC GACAAGGTGC AGAGCTTTCA GGAGGAAATA 
AGCGTCTGGG AGGACGCACA CTCCGCGTCT TTGAAGCGCG CGGCAACCCA GGACGCGCTG 
CTGCAGCAAT GCACGGTTCT CCTCCGTGCC CAGGCAAGCG AGATCAAAGA CCTCATGCAG 
TGCATGGCAG CCTACGCCCA GCCCCTTCAG CAGGCCCGAC AGGCCGTCGA CCAGCTCCAC 
ACCTACGTGT ATGGGGCAAA CACGAGCCGC GAGCGGCAGC GAACGCTTTT TACCCCGGAA 
ACGTGCCAAG AGAATGCCTT CGCGGCAGCA TCGCAGGGCC TCCTGTCCCG TCCACTGGCC 
TCCGTGCACG GATCGAATGT GAGCGCCGTG GGCAACATGA CGGAGAGCAG CGGGCGGCAC 
ATCACCGGCA GCAGCAACCT TCCAGACGGG CAATCACCCA ATCCGCGTCT TCAGCCGGGC 
GTGGACACAG GCCCGCCACA GCCGAGCAGA TCGAGGTCTC TGCCGCAACA CTGTGTTGCA 
AGCGATTACC GTGACAACCT CATGTCGGGG TCGATAAGCG TGTCGCCTGG GACGGCAACG 
GCGCAACAGT GGGTATCGCG CGCCGCCTCG GTAGGACAGA ATGACAGCGG TGCGTCGCAC 
GTCGGCTCCT GTGGCCTGGA TGGAATGACA CCTGCAGCAC CTGCAGAGTG TATCCGTCGC 
CTCGAAGAGG AACTCGCCTC GCTTCAGTCT GACCACGGCG CGCTGCTGCA GCAGCACAAA 
GAGGCGCTGG CGGAGCTGCA GTCGAAGCAG CGCATTCTCG ACCTCCGCCG CGGCCACCTT 
GCCACCGTGA AAGATGAGCT GAAGCGGGAG TACATGATAA ACAAAGAGCT GCGTGAGCGC 
TTGGAGAAGG GGCGGGACAG CCTACGAGGG CCGCTCGAGA TGGCGCGCAA TGATGCCAAC 
TACTGGCGCC GGCGGTACGA GGAGCTTGCG AGCCGAAAGG AGATGCGATG CGCCGCCGAG 
TGCCAGCGTG GCTCCCCCCG CTTTGTCTTT CCACCGCCGC CGCCGCCGCA GCAGCAGCAG 
CAGCCGCACC AGCACCAGCA CCAGCAACAA CACGAGGCCG GGCGCAGCGG CACAATGGAC 
GCGGATGCCT TGAATCTCAT GGCTTTCCAC GCTTCTGCCG ATTCGGCCAG TGGCGGCGCT 
GTGATGGAAA TTAGGAACCG TCCCTCCATC CCGGATATCT CATCCGGCGT CCTGTCGTCG 
AGGGCGTCTG GCGAGAATGT GCTGCTGAGC CTCTCCTCGG CCACAACACC ACTGCGATCG 
ACTGTGGCAC GGCTCACCAC AGAGCTCGAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001465189.1
CDS	1..3156
Translation	MYGQTGSGKTHTMFGEVNGGAWATSCDSGRPSAGVAFNADTNEEDVNEKPKEEDSPAHTRLTEEEHGEMEPHACERQRAQAEGEDEERHEEQKQQQASQQTLQAPGTSPRATRPKLSTYHTKTGHPATSAIATTRSAAAVPPPSATTTSFCRTRATVTPLGELQQINTLQNATGSDAAEYCYAAVNKSTKMCAAATASTSYAPPSSSAAPASPSSAFSRSGPPRCTVRSAAGMRSAFTGDAVSGVIPRAVHDIFDAIAHADPSKEFDVQMFFVEVYMEQIRDLLVPNPSAAMHSSSAGIAPTLPIGPRKLQLREDVSTNSFYVDGCCNPHVSSARDVLHLVKNGLKQRATSATAMNETSSRSHCLLNLTVKSVDRAQGVSTVGKLYLVDLAGSEKVGKTNATGLRLEEAKLINKSLSTLGMVIMSLTDHSATHTPYRDSVLTKILKDSLGGNSHTALVICCSPSPCHAQETLSTLRFGARAKAIENKAVVNRDLTAAQLRRMLETAKGEIERLHVKVQQLSMTSGAVDPCKGTPDFPVVASRKLSSAATATASSMASEADTSRISALLEERATEQARLQNLRAEVAQLHDSLSSAQDIISALQEERDGYIDKVQSFQEEISVWEDAHSASLKRAATQDALLQQCTVLLRAQASEIKDLMQCMAAYAQPLQQARQAVDQLHTYVYGANTSRERQRTLFTPETCQENAFAAASQGLLSRPLASVHGSNVSAVGNMTESSGRHITGSSNLPDGQSPNPRLQPGVDTGPPQPSRSRSLPQHCVASDYRDNLMSGSISVSPGTATAQQWVSRAASVGQNDSGASHVGSCGLDGMTPAAPAECIRRLEEELASLQSDHGALLQQHKEALAELQSKQRILDLRRGHLATVKDELKREYMINKELRERLEKGRDSLRGPLEMARNDANYWRRRYEELASRKEMRCAAECQRGSPRFVFPPPPPPQQQQQPHQHQHQQQHEAGRSGTMDADALNLMAFHASADSASGGAVMEIRNRPSIPDISSGVLSSRASGENVLLSLSSATTPLRSTVARLTTELED

Target ORF information:

RefSeq Version	XM_001465152.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative kinesin heavy chain partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001465152.1

ORF Insert Sequence:

ATGTATGGGC AGACTGGGAG CGGCAAGACG CACACGATGT TCGGAGAAGT GAATGGTGGT 
GCATGGGCCA CAAGCTGCGA CTCAGGCAGG CCCTCTGCAG GCGTCGCCTT CAACGCAGAC 
ACCAACGAGG AGGACGTCAA CGAGAAGCCG AAGGAGGAGG ACAGTCCTGC GCACACGCGC 
CTCACCGAAG AGGAGCACGG AGAGATGGAG CCTCACGCTT GCGAGCGTCA GAGAGCACAA 
GCGGAAGGCG AAGACGAGGA ACGACACGAA GAGCAGAAGC AGCAGCAAGC ATCTCAGCAA 
ACCTTGCAGG CGCCCGGCAC GTCTCCCAGG GCAACGCGGC CGAAACTCAG CACCTACCAT 
ACCAAGACGG GCCACCCGGC GACCTCAGCG ATAGCGACGA CAAGGAGCGC CGCAGCCGTG 
CCACCACCGT CCGCGACGAC GACATCTTTC TGCCGCACCC GCGCCACCGT CACTCCGCTT 
GGCGAGCTGC AGCAAATCAA CACGCTTCAA AACGCTACCG GCAGTGACGC TGCGGAGTAC 
TGCTATGCAG CGGTGAACAA GTCGACAAAA ATGTGTGCCG CCGCCACCGC GTCGACCTCT 
TACGCTCCAC CGTCGTCGTC AGCGGCCCCA GCATCGCCGT CCTCTGCGTT CTCCCGCAGT 
GGACCGCCTC GGTGTACGGT GAGGAGTGCG GCGGGTATGC GGTCCGCCTT TACGGGCGAC 
GCCGTCAGCG GCGTCATACC GCGAGCGGTG CACGACATCT TCGACGCCAT CGCCCACGCA 
GACCCGTCCA AGGAGTTCGA CGTGCAGATG TTCTTTGTGG AGGTGTACAT GGAGCAGATT 
CGCGACCTCC TTGTCCCGAA CCCGTCCGCC GCGATGCATT CATCGTCGGC TGGTATCGCT 
CCAACGCTGC CGATAGGGCC ACGCAAGCTG CAGCTGCGCG AGGATGTTAG CACCAACTCG 
TTCTACGTCG ATGGATGTTG CAACCCGCAT GTGTCGTCTG CCAGGGATGT GCTGCATCTG 
GTGAAGAACG GTCTCAAGCA GCGGGCCACC TCAGCCACGG CGATGAACGA GACGAGCAGC 
CGCAGCCATT GCCTGCTGAA CCTGACGGTG AAGAGCGTCG ACCGCGCGCA GGGGGTGTCG 
ACGGTAGGGA AGCTTTACTT GGTGGACCTC GCTGGTAGCG AGAAGGTTGG GAAAACCAAC 
GCGACAGGGC TGCGCCTAGA GGAGGCAAAG CTGATCAACA AGTCCCTCTC CACTCTTGGC 
ATGGTTATCA TGTCCCTGAC AGATCACAGC GCCACGCACA CGCCGTATCG AGATAGTGTG 
CTGACAAAAA TCTTGAAAGA CTCGCTCGGC GGCAACTCGC ACACGGCCCT TGTGATCTGC 
TGCAGCCCGT CCCCGTGCCA TGCGCAGGAG ACGCTGTCGA CGCTGCGCTT CGGTGCTCGC 
GCGAAGGCTA TCGAGAACAA GGCCGTCGTG AACCGCGACC TCACCGCGGC GCAGCTGCGG 
CGCATGTTGG AGACGGCCAA GGGGGAGATC GAGCGGCTGC ACGTGAAGGT GCAGCAGCTG 
AGCATGACGA GTGGAGCAGT GGACCCCTGT AAAGGGACGC CGGACTTCCC CGTCGTAGCA 
TCGCGAAAGC TGTCGAGCGC CGCCACTGCT ACGGCGTCTT CAATGGCGAG CGAGGCGGAC 
ACGTCCCGCA TCAGTGCACT GCTGGAGGAG CGTGCGACGG AGCAGGCGCG GTTGCAGAAT 
CTCCGCGCTG AGGTGGCGCA GCTGCATGAC AGCCTCAGCA GCGCCCAGGA CATCATCAGT 
GCCTTGCAGG AGGAGCGCGA CGGCTACATC GACAAGGTGC AGAGCTTTCA GGAGGAAATA 
AGCGTCTGGG AGGACGCACA CTCCGCGTCT TTGAAGCGCG CGGCAACCCA GGACGCGCTG 
CTGCAGCAAT GCACGGTTCT CCTCCGTGCC CAGGCAAGCG AGATCAAAGA CCTCATGCAG 
TGCATGGCAG CCTACGCCCA GCCCCTTCAG CAGGCCCGAC AGGCCGTCGA CCAGCTCCAC 
ACCTACGTGT ATGGGGCAAA CACGAGCCGC GAGCGGCAGC GAACGCTTTT TACCCCGGAA 
ACGTGCCAAG AGAATGCCTT CGCGGCAGCA TCGCAGGGCC TCCTGTCCCG TCCACTGGCC 
TCCGTGCACG GATCGAATGT GAGCGCCGTG GGCAACATGA CGGAGAGCAG CGGGCGGCAC 
ATCACCGGCA GCAGCAACCT TCCAGACGGG CAATCACCCA ATCCGCGTCT TCAGCCGGGC 
GTGGACACAG GCCCGCCACA GCCGAGCAGA TCGAGGTCTC TGCCGCAACA CTGTGTTGCA 
AGCGATTACC GTGACAACCT CATGTCGGGG TCGATAAGCG TGTCGCCTGG GACGGCAACG 
GCGCAACAGT GGGTATCGCG CGCCGCCTCG GTAGGACAGA ATGACAGCGG TGCGTCGCAC 
GTCGGCTCCT GTGGCCTGGA TGGAATGACA CCTGCAGCAC CTGCAGAGTG TATCCGTCGC 
CTCGAAGAGG AACTCGCCTC GCTTCAGTCT GACCACGGCG CGCTGCTGCA GCAGCACAAA 
GAGGCGCTGG CGGAGCTGCA GTCGAAGCAG CGCATTCTCG ACCTCCGCCG CGGCCACCTT 
GCCACCGTGA AAGATGAGCT GAAGCGGGAG TACATGATAA ACAAAGAGCT GCGTGAGCGC 
TTGGAGAAGG GGCGGGACAG CCTACGAGGG CCGCTCGAGA TGGCGCGCAA TGATGCCAAC 
TACTGGCGCC GGCGGTACGA GGAGCTTGCG AGCCGAAAGG AGATGCGATG CGCCGCCGAG 
TGCCAGCGTG GCTCCCCCCG CTTTGTCTTT CCACCGCCGC CGCCGCCGCA GCAGCAGCAG 
CAGCCGCACC AGCACCAGCA CCAGCAACAA CACGAGGCCG GGCGCAGCGG CACAATGGAC 
GCGGATGCCT TGAATCTCAT GGCTTTCCAC GCTTCTGCCG ATTCGGCCAG TGGCGGCGCT 
GTGATGGAAA TTAGGAACCG TCCCTCCATC CCGGATATCT CATCCGGCGT CCTGTCGTCG 
AGGGCGTCTG GCGAGAATGT GCTGCTGAGC CTCTCCTCGG CCACAACACC ACTGCGATCG 
ACTGTGGCAC GGCTCACCAC AGAGCTCGAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_20_0700 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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