FFAR2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FFAR2
Entrez Gene ID	2867
Full Name	free fatty acid receptor 2
Synonyms	FFA2R,GPR43
General protein information	Preferred Names free fatty acid receptor 2 Names free fatty acid receptor 2 G-protein coupled receptor 43 fatty acid receptor 2 free fatty acid activated receptor 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009].
Disorder MIM:	603823

mRNA and Protein(s)

mRNA	Protein	Name
XM_017026709.1	XP_016882198.1	free fatty acid receptor 2 isoform X1
NM_001370087.1	NP_001357016.1	free fatty acid receptor 2
XM_017026711.1	XP_016882200.1	free fatty acid receptor 2 isoform X1
NM_005306.2	NP_005297.1	free fatty acid receptor 2
NM_005306.3	NP_005297.1	free fatty acid receptor 2
XM_017026710.2	XP_016882199.1	free fatty acid receptor 2 isoform X1
XM_017026710.1	XP_016882199.1	free fatty acid receptor 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FFAR2	XP_005616767.1
Mus musculus (house mouse)	Ffar2	NP_001161981.1
Rattus norvegicus (Norway rat)	Ffar2	NP_001005877.1
Gallus gallus (chicken)	LOC429354	XP_426910.4
Gallus gallus (chicken)	LOC429347	XP_004950704.1
Gallus gallus (chicken)	LOC101749263	XP_004950270.1
Danio rerio (zebrafish)	LOC100004095	XP_001920566.1
Gallus gallus (chicken)	LOC100858431	XP_004950711.1
Gallus gallus (chicken)	LOC100859086	XP_003643854.2
Pan troglodytes (chimpanzee)	FFAR2	XP_512587.2
Macaca mulatta (Rhesus monkey)	FFAR2	XP_001095000.1
Gallus gallus (chicken)	LOC777322	XP_004951012.1
Gallus gallus (chicken)	LOC101747502	XP_004950186.1
Xenopus tropicalis (tropical clawed frog)	LOC100486488	XP_002938241.1
Bos taurus (cattle)	FFAR2	NP_001157256.1
Gallus gallus (chicken)	LOC430168	XP_004950966.1
Danio rerio (zebrafish)	si:dkey-211g8.6	NP_001076364.1
Danio rerio (zebrafish)	LOC571502	XP_700191.4
Gallus gallus (chicken)	LOC101747469	XP_004950708.1
Gallus gallus (chicken)	LOC101749787	XP_004950719.1
Gallus gallus (chicken)	LOC100858117	XP_003643742.2
Danio rerio (zebrafish)	LOC100004033	XP_001343441.3
Gallus gallus (chicken)	LOC776487	XP_001236005.2
Gallus gallus (chicken)	LOC430071	XP_427629.3
Gallus gallus (chicken)	LOC429574	XP_004950637.1
Gallus gallus (chicken)	LOC100857826	XP_004950188.1
Gallus gallus (chicken)	LOC100858697	XP_003644001.1
Homo sapiens (human)	FFAR2	NP_005297.1
Gallus gallus (chicken)	LOC777462	XP_001236909.2
Gallus gallus (chicken)	LOC430746	XP_004951134.1
Gallus gallus (chicken)	LOC429280	XP_004950908.1
Gallus gallus (chicken)	LOC101752209	XP_004950707.1
Gallus gallus (chicken)	LOC101747578	XP_004950709.1
Gallus gallus (chicken)	LOC100857419	XP_003643822.2
Gallus gallus (chicken)	LOC100859180	XP_004950219.1
Gallus gallus (chicken)	LOC100859369	XP_004950727.1

Gene Ontology
Bibliography

Related articles in PubMed

Short chain fatty acids stimulate insulin secretion and reduce apoptosis in mouse and human islets in vitro: Role of free fatty acid receptor 2.
Pingitore A, Gonzalez-Abuin N, Ruz-Maldonado I, Huang GC, Frost G, Persaud SJ
Diabetes, obesity & metabolism21(2)330-339(2019 02)

Characterisation of small molecule ligands 4CMTB and 2CTAP as modulators of human FFA2 receptor signalling.
Schofield ZV, Croker D, Robertson AAB, Massey NL, Donovan C, Tee E, Edwards D, Woodruff TM, Halai R, Hansbro PM, Cooper MA
Scientific reports8(1)17819(2018 12)

Similarities and differences between the responses induced in human phagocytes through activation of the medium chain fatty acid receptor GPR84 and the short chain fatty acid receptor FFA2R.
Sundqvist M, Christenson K, Holdfeldt A, Gabl M, Mårtensson J, Björkman L, Dieckmann R, Dahlgren C, Forsman H
Biochimica et biophysica acta. Molecular cell research1865(5)695-708(2018 May)

Increased whole blood FFA2/GPR43 receptor expression is associated with increased 30-day survival in patients with sepsis.
Carr ZJ, Van De Louw A, Fehr G, Li JD, Kunselman A, Ruiz-Velasco V
BMC research notes11(1)41(2018 Jan)

A single extracellular amino acid in Free Fatty Acid Receptor 2 defines antagonist species selectivity and G protein selection bias.
Sergeev E, Hansen AH, Bolognini D, Kawakami K, Kishi T, Aoki J, Ulven T, Inoue A, Hudson BD, Milligan G
Scientific reports7(1)13741(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FFAR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FFAR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31860	NM_001370087.1 Latest version!	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu31860	NM_005306.3 Latest version!	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu31860	XM_017026711.1 Latest version!	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu31860	XM_017026709.1 Latest version!	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu31860	XM_017026710.2 Latest version!	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
View Old Accession Versions >>
OHu31860	NM_005306.2	Homo sapiens free fatty acid receptor 2 (FFAR2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu31860	XM_017026710.1	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FFAR2 ( NM_001370087.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357016.1 Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant 2, mRNA.

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CloneID	OHu31860
Clone ID Related Accession (Same CDS sequence)	XM_017026709.1 , NM_001370087.1 , XM_017026711.1 , NM_005306.2 , NM_005306.3 , XM_017026710.2 , XM_017026710.1
Accession Version	NM_001370087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572105600000
Organism	Homo sapiens(human)
Product	free fatty acid receptor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002511.1. On Apr 26, 2019 this sequence version replaced XM_017026710.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138517.119558.1, DRR138517.507566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357016.1
CDS	125..1117
Translation	MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE

Target ORF information:

RefSeq Version	NM_001370087.1
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370087.1

ORF Insert Sequence:

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR2 ( NM_005306.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005297.1 Homo sapiens free fatty acid receptor 2 (FFAR2), mRNA.

Price & Availability↑

CloneID	OHu31860
Clone ID Related Accession (Same CDS sequence)	XM_017026709.1 , NM_001370087.1 , XM_017026711.1 , NM_005306.2 , NM_005306.3 , XM_017026710.2 , XM_017026710.1
Accession Version	NM_005306.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550505600000
Organism	Homo sapiens(human)
Product	free fatty acid receptor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC096198.3, CK819813.1 and CA309917.1. On Apr 23, 2009 this sequence version replaced NM_005306.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005297.1
CDS	1..993
Translation	MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE

Target ORF information:

RefSeq Version	NM_005306.2
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 2 (FFAR2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005306.2

ORF Insert Sequence:

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR2 ( NM_005306.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005297.1 Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31860
Clone ID Related Accession (Same CDS sequence)	XM_017026709.1 , NM_001370087.1 , XM_017026711.1 , NM_005306.2 , NM_005306.3 , XM_017026710.2 , XM_017026710.1
Accession Version	NM_005306.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572105600000
Organism	Homo sapiens(human)
Product	free fatty acid receptor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002511.1. On or before Apr 26, 2019 this sequence version replaced XM_017026709.1, NM_005306.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.264057.1, SRR1163657.295167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005297.1
CDS	248..1240
Translation	MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE

Target ORF information:

RefSeq Version	NM_005306.3
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005306.3

ORF Insert Sequence:

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR2 ( XM_017026709.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882198.1 Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu31860
Clone ID Related Accession (Same CDS sequence)	XM_017026709.1 , NM_001370087.1 , XM_017026711.1 , NM_005306.2 , NM_005306.3 , XM_017026710.2 , XM_017026710.1
Accession Version	XM_017026709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	free fatty acid receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882198.1
CDS	540..1532
Translation	MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE

Target ORF information:

RefSeq Version	XM_017026709.1
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026709.1

ORF Insert Sequence:

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR2 ( XM_017026711.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882200.1 Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu31860
Clone ID Related Accession (Same CDS sequence)	XM_017026709.1 , NM_001370087.1 , XM_017026711.1 , NM_005306.2 , NM_005306.3 , XM_017026710.2 , XM_017026710.1
Accession Version	XM_017026711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	free fatty acid receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882200.1
CDS	175..1167
Translation	MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE

Target ORF information:

RefSeq Version	XM_017026711.1
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026711.1

ORF Insert Sequence:

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR2 ( XM_017026710.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882199.1 Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu31860
Clone ID Related Accession (Same CDS sequence)	XM_017026709.1 , NM_001370087.1 , XM_017026711.1 , NM_005306.2 , NM_005306.3 , XM_017026710.2 , XM_017026710.1
Accession Version	XM_017026710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	free fatty acid receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017026710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882199.1
CDS	416..1408
Translation	MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE

Target ORF information:

RefSeq Version	XM_017026710.2
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026710.2

ORF Insert Sequence:

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR2 ( XM_017026710.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882199.1 Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu31860
Clone ID Related Accession (Same CDS sequence)	XM_017026709.1 , NM_001370087.1 , XM_017026711.1 , NM_005306.2 , NM_005306.3 , XM_017026710.2 , XM_017026710.1
Accession Version	XM_017026710.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	free fatty acid receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882199.1
CDS	417..1409
Translation	MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE

Target ORF information:

RefSeq Version	XM_017026710.1
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 2 (FFAR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026710.1

ORF Insert Sequence:

ATGCTGCCGG ACTGGAAGAG CTCCTTGATC CTCATGGCTT ACATCATCAT CTTCCTCACT 
GGCCTCCCTG CCAACCTCCT GGCCCTGCGG GCCTTTGTGG GGCGGATCCG CCAGCCCCAG 
CCTGCACCTG TGCACATCCT CCTGCTGAGC CTGACGCTGG CCGACCTCCT CCTGCTGCTG 
CTGCTGCCCT TCAAGATCAT CGAGGCTGCG TCGAACTTCC GCTGGTACCT GCCCAAGGTC 
GTCTGCGCCC TCACGAGTTT TGGCTTCTAC AGCAGCATCT ACTGCAGCAC GTGGCTCCTG 
GCGGGCATCA GCATCGAGCG CTACCTGGGA GTGGCTTTCC CCGTGCAGTA CAAGCTCTCC 
CGCCGGCCTC TGTATGGAGT GATTGCAGCT CTGGTGGCCT GGGTTATGTC CTTTGGTCAC 
TGCACCATCG TGATCATCGT TCAATACTTG AACACGACTG AGCAGGTCAG AAGTGGCAAT 
GAAATTACCT GCTACGAGAA CTTCACCGAT AACCAGTTGG ACGTGGTGCT GCCCGTGCGG 
CTGGAGCTGT GCCTGGTGCT CTTCTTCATC CCCATGGCAG TCACCATCTT CTGCTACTGG 
CGTTTTGTGT GGATCATGCT CTCCCAGCCC CTTGTGGGGG CCCAGAGGCG GCGCCGAGCC 
GTGGGGCTGG CTGTGGTGAC GCTGCTCAAT TTCCTGGTGT GCTTCGGACC TTACAACGTG 
TCCCACCTGG TGGGGTATCA CCAGAGAAAA AGCCCCTGGT GGCGGTCAAT AGCCGTGGTG 
TTCAGTTCAC TCAACGCCAG TCTGGACCCC CTGCTCTTCT ATTTCTCTTC TTCAGTGGTG 
CGCAGGGCAT TTGGGAGAGG GCTGCAGGTG CTGCGGAATC AGGGCTCCTC CCTGTTGGGA 
CGCAGAGGCA AAGACACAGC AGAGGGGACA AATGAGGACA GGGGTGTGGG TCAAGGAGAA 
GGGATGCCAA GTTCGGACTT CACTACAGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Short chain fatty acids stimulate insulin secretion and reduce apoptosis in mouse and human islets in vitro: Role of free fatty acid receptor 2.
Diabetes, obesity & metabolism21(2)330-339(2019 02)
Pingitore A,Gonzalez-Abuin N,Ruz-Maldonado I,Huang GC,Frost G,Persaud SJ

Characterisation of small molecule ligands 4CMTB and 2CTAP as modulators of human FFA2 receptor signalling.
Scientific reports8(1)17819(2018 12)
Schofield ZV,Croker D,Robertson AAB,Massey NL,Donovan C,Tee E,Edwards D,Woodruff TM,Halai R,Hansbro PM,Cooper MA

Similarities and differences between the responses induced in human phagocytes through activation of the medium chain fatty acid receptor GPR84 and the short chain fatty acid receptor FFA2R.
Biochimica et biophysica acta. Molecular cell research1865(5)695-708(2018 May)
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