WNK3 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	WNK3
Entrez Gene ID	101093000
Full Name	WNK lysine deficient protein kinase 3
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011291755.2	XP_011290057.1	serine/threonine-protein kinase WNK3 isoform X1
XM_004000545.3	XP_004000594.1	serine/threonine-protein kinase WNK3 isoform X2
XM_019823859.1	XP_019679418.1	serine/threonine-protein kinase WNK3 isoform X1
XM_019823860.2	XP_019679419.1	serine/threonine-protein kinase WNK3 isoform X1
XM_011291755.3	XP_011290057.1	serine/threonine-protein kinase WNK3 isoform X1
XM_019823858.1	XP_019679417.1	serine/threonine-protein kinase WNK3 isoform X1
XM_019823860.1	XP_019679419.1	serine/threonine-protein kinase WNK3 isoform X1

Pathways from BioSystems
Gene Ontology

The following WNK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb23760	XM_004000545.3 Latest version!	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb31125	XM_019823859.1 Latest version!	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb31125	XM_019823858.1 Latest version!	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb31125	XM_011291755.3 Latest version!	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb31125	XM_019823860.2 Latest version!	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OFb31125	XM_011291755.2	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb31125	XM_019823860.1	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WNK3 ( XM_004000545.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_004000594.1 Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X5, mRNA.

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CloneID	OFb23760
Clone ID Related Accession (Same CDS sequence)	XM_004000545.3
Accession Version	XM_004000545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	serine/threonine-protein kinase WNK3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_004000545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGTGGAA ACAGAAGATA TGAGATCAGC AGTTGCTCCT 
GATCCCATTC CTCTGACAGG AGAGTCCACA GCTGATACTA GGGCTTTGAG TAGATGCAAA 
GTGATGAGTG GATCATTTCA GCGGGGTCGG TTCCAGGTGA TTACAGTTCC TCAGCAGCAG 
TCAGTGAAAG TGACATCTTT TGGAATAGAA CACATACCAG CATTCAATAA AATGACAACC 
CATTCTAGTG AAGAAGTTCT AGCCTTTACT GAAATAGCCA AGTCTCAGTT AGTGAAAATG 
GAAACTTCCA TGTGCAATCC ACAAACTTCA CTTCCTTCTC AGAAGTTACG AGCTCATCGT 
GAAACCTTTA AAGAAGATAA AAGAATTGCC AAACAAGGTG ACAACTACTC ATCTTTCAAC 
ACAGCTTGTG AGACTGATGT ATCTTTGATG ACCCCAGAAA AGGAACCGGA AGAAAACTCA 
GCCATGGGAA GTAGTATGCA TTCTGGACTT GAACTATTGC TTAAAGAGAG AGAGACATTT 
ACTGCTGGGA AACATCCTAG CTCTGATAGT GAATTTTCAG CCACTCTTGC TGGCAGAGGG 
AGGTCAGTGG CAAAGACTGC TCCAGTGAGT GATCAGTGCT TACCACTCCG TGAAGAAAAA 
GCCTTTGCTC AAACACAGAG CTCACTCTTC TATTCACCAT CTTCTCCAAT GAGCAGTGAT 
GATGACTCAG AAATAGAGGA TGAGGATTTG AAGGTGGAGC TTCAAAGATT ACGAGAAAAA 
CACATTCAGG AGGTGGTAAA TCTTCAAACC CAGCAGAATA AGGAGCTGCA AGAGCTCTAT 
GAACGCCTTC GGGCAATTAA AGATAGCAAA ATCCAATCAT CAGAGGCTCC TCTGCCACCA 
TCATCACCAC GTAGACCAAG ATCTTTCAAA AGCAAACTTC GCAGCCGCCC CCAGTCCTGG 
ACACATGTGG ACAATGTCGC AGTTGCCACA GATCCGCTCT GTGTGGAGAG TAATATAGCA 
TCATGCCAGC AATCTCCAGC TAGTAAAAAA GGCATGTTCA CAGATGACTT ACATAAACTG 
GTGGATGACT GGACAAAGGA AACAGTTGGA AATTCTCTTA TTAAGCCAAG TTTAAACCAA 
CTCAAACAGA GTCAACACAA ACTAGAGACA GAAAACTGGA ACAAAGTATA TGAAAATACT 
CCATCTACTA TGGGCTACAC ATCAACATGG ATTTCTTCTC TGTCCCAAAT CCGTGGAGCT 
GTCCCAACCT CCTTGCCACA AGGACTCTCA CTCACTTCAT TTCCTGGGAC ATTATCATCA 
TATGGAATGC CCCATGTCTG TCAGTATAAT GCTGTGGGGG GGCCAGGATA CCCAGTACAA 
TGGGTAGGAA TTTCAGGAAC ACCACAACAG TCTGTAGTAA TTCCGACTCA ATCTGGGGGA 
CCATTCCAGC CGGGAATGAA TTTACAGGCA TTTCCAGCTT CATCAGTGCA TAATCCATCT 
GCCATCCCGC CTGGTCCCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004000594.1
CDS	499..5742
Translation	MATDSGEPASTEDSEKPDGVSLEDRAPQVAATLTVEARLKEKNSTFSATGETTERKRFFRKSVEMMEDDKVAESSSKDERMKTATNIPRVEKLPTNVLGGGQEVKYEQCSKATSESSKDCLKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTSEEVAYEMVRSGFFHESDSKAVAKSIRDRVTLIKKTREKKPAGCLEERRDSQCKSVGNVLPQHQNTTLSPAPAQHTGAECEETEVDQHVRQQFLQRKPQQHCSSVTGDNLSEAGAGSAIHSDTSSHPSVAYSSDQKMGSQVVSNIPQAEINVPGMIYPSQQRVGHYQQISGLQQQPKLSQHHILPLVQGQSTVLPVHVLGPPVVSQPQVSPLTVQTVSQVKPISQAVGVEQQATLLKPDLVRSLNQDVAAVKENINTPDNLYGNGKQDRSKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDADGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHAHSAVDRAIGTDSIIVESNSSQTGSSEQVQINSASTQTSNESAPQSSPVGRWRFCINQTIRNREAQSPPLQQSMSTVPGLPLLSGPRTTSNKEISQDTLLTLENNPCQRVLFASKSELKDVVDGKISEYASVDTKQQAVFFQVEDDRQIMASVASSSTHTATSVRAVPVECEGLTNQAGIFLPAYPCHQAASQADVLMVPPGESTQIAGNSLTTLAFMSDQKPQSLAVQQPTMDAELISQEGETTVNTEASSPKMVIPTQTPGLELTTLLPSTVLESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVPEVIGISHSGIQDSPAQSPNFQQTGSKILSNVAVSQPANISVFKRDLNVITSIPSEICLHEMSPDASLPGDPETYPAAVSSGGAIHLQTGVETEDMRSAVAPDPIPLTGESTADTRALSRCKVMSGSFQRGRFQVITVPQQQSVKVTSFGIEHIPAFNKMTTHSSEEVLAFTEIAKSQLVKMETSMCNPQTSLPSQKLRAHRETFKEDKRIAKQGDNYSSFNTACETDVSLMTPEKEPEENSAMGSSMHSGLELLLKERETFTAGKHPSSDSEFSATLAGRGRSVAKTAPVSDQCLPLREEKAFAQTQSSLFYSPSSPMSSDDDSEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRAIKDSKIQSSEAPLPPSSPRRPRSFKSKLRSRPQSWTHVDNVAVATDPLCVESNIASCQQSPASKKGMFTDDLHKLVDDWTKETVGNSLIKPSLNQLKQSQHKLETENWNKVYENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLTSFPGTLSSYGMPHVCQYNAVGGPGYPVQWVGISGTPQQSVVIPTQSGGPFQPGMNLQAFPASSVHNPSAIPPGPK

Target ORF information:

RefSeq Version	XM_004000545.3
Organism	Felis catus(domestic cat)
Definition	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004000545.3

ORF Insert Sequence:

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGTGGAA ACAGAAGATA TGAGATCAGC AGTTGCTCCT 
GATCCCATTC CTCTGACAGG AGAGTCCACA GCTGATACTA GGGCTTTGAG TAGATGCAAA 
GTGATGAGTG GATCATTTCA GCGGGGTCGG TTCCAGGTGA TTACAGTTCC TCAGCAGCAG 
TCAGTGAAAG TGACATCTTT TGGAATAGAA CACATACCAG CATTCAATAA AATGACAACC 
CATTCTAGTG AAGAAGTTCT AGCCTTTACT GAAATAGCCA AGTCTCAGTT AGTGAAAATG 
GAAACTTCCA TGTGCAATCC ACAAACTTCA CTTCCTTCTC AGAAGTTACG AGCTCATCGT 
GAAACCTTTA AAGAAGATAA AAGAATTGCC AAACAAGGTG ACAACTACTC ATCTTTCAAC 
ACAGCTTGTG AGACTGATGT ATCTTTGATG ACCCCAGAAA AGGAACCGGA AGAAAACTCA 
GCCATGGGAA GTAGTATGCA TTCTGGACTT GAACTATTGC TTAAAGAGAG AGAGACATTT 
ACTGCTGGGA AACATCCTAG CTCTGATAGT GAATTTTCAG CCACTCTTGC TGGCAGAGGG 
AGGTCAGTGG CAAAGACTGC TCCAGTGAGT GATCAGTGCT TACCACTCCG TGAAGAAAAA 
GCCTTTGCTC AAACACAGAG CTCACTCTTC TATTCACCAT CTTCTCCAAT GAGCAGTGAT 
GATGACTCAG AAATAGAGGA TGAGGATTTG AAGGTGGAGC TTCAAAGATT ACGAGAAAAA 
CACATTCAGG AGGTGGTAAA TCTTCAAACC CAGCAGAATA AGGAGCTGCA AGAGCTCTAT 
GAACGCCTTC GGGCAATTAA AGATAGCAAA ATCCAATCAT CAGAGGCTCC TCTGCCACCA 
TCATCACCAC GTAGACCAAG ATCTTTCAAA AGCAAACTTC GCAGCCGCCC CCAGTCCTGG 
ACACATGTGG ACAATGTCGC AGTTGCCACA GATCCGCTCT GTGTGGAGAG TAATATAGCA 
TCATGCCAGC AATCTCCAGC TAGTAAAAAA GGCATGTTCA CAGATGACTT ACATAAACTG 
GTGGATGACT GGACAAAGGA AACAGTTGGA AATTCTCTTA TTAAGCCAAG TTTAAACCAA 
CTCAAACAGA GTCAACACAA ACTAGAGACA GAAAACTGGA ACAAAGTATA TGAAAATACT 
CCATCTACTA TGGGCTACAC ATCAACATGG ATTTCTTCTC TGTCCCAAAT CCGTGGAGCT 
GTCCCAACCT CCTTGCCACA AGGACTCTCA CTCACTTCAT TTCCTGGGAC ATTATCATCA 
TATGGAATGC CCCATGTCTG TCAGTATAAT GCTGTGGGGG GGCCAGGATA CCCAGTACAA 
TGGGTAGGAA TTTCAGGAAC ACCACAACAG TCTGTAGTAA TTCCGACTCA ATCTGGGGGA 
CCATTCCAGC CGGGAATGAA TTTACAGGCA TTTCCAGCTT CATCAGTGCA TAATCCATCT 
GCCATCCCGC CTGGTCCCAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_011291755.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_011290057.1 Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.

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CloneID	OFb31125
Clone ID Related Accession (Same CDS sequence)	XM_011291755.2 , XM_019823859.1 , XM_019823860.2 , XM_011291755.3 , XM_019823858.1 , XM_019823860.1
Accession Version	XM_011291755.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_011291755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011290057.1
CDS	501..5885
Translation	MATDSGEPASTEDSEKPDGVSLEDRAPQVAATLTVEARLKEKNSTFSATGETTERKRFFRKSVEMMEDDKVAESSSKDERMKTATNIPRVEKLPTNVLGGGQEVKYEQCSKATSESSKDCLKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTSEEVAYEMVRSGFFHESDSKAVAKSIRDRVTLIKKTREKKPAGCLEERRDSQCKSVGNVLPQHQNTTLSPAPAQHTGAECEETEVDQHVRQQFLQRKPQQHCSSVTGDNLSEAGAGSAIHSDTSSHPSVAYSSDQKMGSQVVSNIPQAEINVPGMIYPSQQRVGHYQQISGLQQQPKLSQHHILPLVQGQSTVLPVHVLGPPVVSQPQVSPLTVQTVSQVKPISQAVGVEQQATLLKPDLVRSLNQDVAAVKENINTPDNLYGNGKQDRSKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDADGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHAHSAVDRAIGTDSIIVESNSSQTGSSEQVQINSASTQTSNESAPQSSPVGRWRFCINQTIRNREAQSPPLQQSMSTVPGLPLLSGPRTTSNKEISQDTLLTLENNPCQRVLFASKSELKDVVDGKISEYASVDTKQQAVFFQVEDDRQIMASVASSSTHTATSVRAVPVECEGLTNQAGIFLPAYPCHQAASQADVLMVPPGESTQIAGNSLTTLAFMSDQKPQSLAVQQPTMDAELISQEGETTVNTEASSPKMVIPTQTPGLELTTLLPSTVLESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVPEVIGISHSGIQDSPAQSPNFQQTGSKILSNVAVSQPANISVFKRDLNVITSIPSEICLHEMSPDASLPGDPETYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDMRSAVAPDPIPLTGESTADTRALSRCKVMSGSFQRGRFQVITVPQQQSVKVTSFGIEHIPAFNKMTTHSSEEVLAFTEIAKSQLVKMETSMCNPQTSLPSQKLRAHRETFKEDKRIAKQGDNYSSFNTACETDVSLMTPEKEPEENSAMGSSMHSGLELLLKERETFTAGKHPSSDSEFSATLAGRGRSVAKTAPVSDQCLPLREEKAFAQTQSSLFYSPSSPMSSDDDSEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRAIKDSKIQSSEAPLPPSSPRRPRSFKSKLRSRPQSWTHVDNVAVATDPLCVESNIASCQQSPASKKGMFTDDLHKLVDDWTKETVGNSLIKPSLNQLKQSQHKLETENWNKVYENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLTSFPGTLSSYGMPHVCQYNAVGGPGYPVQWVGISGTPQQSVVIPTQSGGPFQPGMNLQAFPASSVHNPSAIPPGPK

Target ORF information:

RefSeq Version	XM_011291755.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011291755.2

ORF Insert Sequence:

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_019823859.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019679418.1 Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X3, mRNA.

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CloneID	OFb31125
Clone ID Related Accession (Same CDS sequence)	XM_011291755.2 , XM_019823859.1 , XM_019823860.2 , XM_011291755.3 , XM_019823858.1 , XM_019823860.1
Accession Version	XM_019823859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019679418.1
CDS	215..5599
Translation	MATDSGEPASTEDSEKPDGVSLEDRAPQVAATLTVEARLKEKNSTFSATGETTERKRFFRKSVEMMEDDKVAESSSKDERMKTATNIPRVEKLPTNVLGGGQEVKYEQCSKATSESSKDCLKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTSEEVAYEMVRSGFFHESDSKAVAKSIRDRVTLIKKTREKKPAGCLEERRDSQCKSVGNVLPQHQNTTLSPAPAQHTGAECEETEVDQHVRQQFLQRKPQQHCSSVTGDNLSEAGAGSAIHSDTSSHPSVAYSSDQKMGSQVVSNIPQAEINVPGMIYPSQQRVGHYQQISGLQQQPKLSQHHILPLVQGQSTVLPVHVLGPPVVSQPQVSPLTVQTVSQVKPISQAVGVEQQATLLKPDLVRSLNQDVAAVKENINTPDNLYGNGKQDRSKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDADGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHAHSAVDRAIGTDSIIVESNSSQTGSSEQVQINSASTQTSNESAPQSSPVGRWRFCINQTIRNREAQSPPLQQSMSTVPGLPLLSGPRTTSNKEISQDTLLTLENNPCQRVLFASKSELKDVVDGKISEYASVDTKQQAVFFQVEDDRQIMASVASSSTHTATSVRAVPVECEGLTNQAGIFLPAYPCHQAASQADVLMVPPGESTQIAGNSLTTLAFMSDQKPQSLAVQQPTMDAELISQEGETTVNTEASSPKMVIPTQTPGLELTTLLPSTVLESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVPEVIGISHSGIQDSPAQSPNFQQTGSKILSNVAVSQPANISVFKRDLNVITSIPSEICLHEMSPDASLPGDPETYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDMRSAVAPDPIPLTGESTADTRALSRCKVMSGSFQRGRFQVITVPQQQSVKVTSFGIEHIPAFNKMTTHSSEEVLAFTEIAKSQLVKMETSMCNPQTSLPSQKLRAHRETFKEDKRIAKQGDNYSSFNTACETDVSLMTPEKEPEENSAMGSSMHSGLELLLKERETFTAGKHPSSDSEFSATLAGRGRSVAKTAPVSDQCLPLREEKAFAQTQSSLFYSPSSPMSSDDDSEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRAIKDSKIQSSEAPLPPSSPRRPRSFKSKLRSRPQSWTHVDNVAVATDPLCVESNIASCQQSPASKKGMFTDDLHKLVDDWTKETVGNSLIKPSLNQLKQSQHKLETENWNKVYENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLTSFPGTLSSYGMPHVCQYNAVGGPGYPVQWVGISGTPQQSVVIPTQSGGPFQPGMNLQAFPASSVHNPSAIPPGPK

Target ORF information:

RefSeq Version	XM_019823859.1
Organism	Felis catus(domestic cat)
Definition	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019823859.1

ORF Insert Sequence:

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_019823860.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019679419.1 Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.

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CloneID	OFb31125
Clone ID Related Accession (Same CDS sequence)	XM_011291755.2 , XM_019823859.1 , XM_019823860.2 , XM_011291755.3 , XM_019823858.1 , XM_019823860.1
Accession Version	XM_019823860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019823860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019679419.1
CDS	381..5765
Translation	MATDSGEPASTEDSEKPDGVSLEDRAPQVAATLTVEARLKEKNSTFSATGETTERKRFFRKSVEMMEDDKVAESSSKDERMKTATNIPRVEKLPTNVLGGGQEVKYEQCSKATSESSKDCLKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTSEEVAYEMVRSGFFHESDSKAVAKSIRDRVTLIKKTREKKPAGCLEERRDSQCKSVGNVLPQHQNTTLSPAPAQHTGAECEETEVDQHVRQQFLQRKPQQHCSSVTGDNLSEAGAGSAIHSDTSSHPSVAYSSDQKMGSQVVSNIPQAEINVPGMIYPSQQRVGHYQQISGLQQQPKLSQHHILPLVQGQSTVLPVHVLGPPVVSQPQVSPLTVQTVSQVKPISQAVGVEQQATLLKPDLVRSLNQDVAAVKENINTPDNLYGNGKQDRSKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDADGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHAHSAVDRAIGTDSIIVESNSSQTGSSEQVQINSASTQTSNESAPQSSPVGRWRFCINQTIRNREAQSPPLQQSMSTVPGLPLLSGPRTTSNKEISQDTLLTLENNPCQRVLFASKSELKDVVDGKISEYASVDTKQQAVFFQVEDDRQIMASVASSSTHTATSVRAVPVECEGLTNQAGIFLPAYPCHQAASQADVLMVPPGESTQIAGNSLTTLAFMSDQKPQSLAVQQPTMDAELISQEGETTVNTEASSPKMVIPTQTPGLELTTLLPSTVLESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVPEVIGISHSGIQDSPAQSPNFQQTGSKILSNVAVSQPANISVFKRDLNVITSIPSEICLHEMSPDASLPGDPETYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDMRSAVAPDPIPLTGESTADTRALSRCKVMSGSFQRGRFQVITVPQQQSVKVTSFGIEHIPAFNKMTTHSSEEVLAFTEIAKSQLVKMETSMCNPQTSLPSQKLRAHRETFKEDKRIAKQGDNYSSFNTACETDVSLMTPEKEPEENSAMGSSMHSGLELLLKERETFTAGKHPSSDSEFSATLAGRGRSVAKTAPVSDQCLPLREEKAFAQTQSSLFYSPSSPMSSDDDSEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRAIKDSKIQSSEAPLPPSSPRRPRSFKSKLRSRPQSWTHVDNVAVATDPLCVESNIASCQQSPASKKGMFTDDLHKLVDDWTKETVGNSLIKPSLNQLKQSQHKLETENWNKVYENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLTSFPGTLSSYGMPHVCQYNAVGGPGYPVQWVGISGTPQQSVVIPTQSGGPFQPGMNLQAFPASSVHNPSAIPPGPK

Target ORF information:

RefSeq Version	XM_019823860.2
Organism	Felis catus(domestic cat)
Definition	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019823860.2

ORF Insert Sequence:

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_011291755.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_011290057.1 Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.

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CloneID	OFb31125
Clone ID Related Accession (Same CDS sequence)	XM_011291755.2 , XM_019823859.1 , XM_019823860.2 , XM_011291755.3 , XM_019823858.1 , XM_019823860.1
Accession Version	XM_011291755.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_011291755.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011290057.1
CDS	500..5884
Translation	MATDSGEPASTEDSEKPDGVSLEDRAPQVAATLTVEARLKEKNSTFSATGETTERKRFFRKSVEMMEDDKVAESSSKDERMKTATNIPRVEKLPTNVLGGGQEVKYEQCSKATSESSKDCLKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTSEEVAYEMVRSGFFHESDSKAVAKSIRDRVTLIKKTREKKPAGCLEERRDSQCKSVGNVLPQHQNTTLSPAPAQHTGAECEETEVDQHVRQQFLQRKPQQHCSSVTGDNLSEAGAGSAIHSDTSSHPSVAYSSDQKMGSQVVSNIPQAEINVPGMIYPSQQRVGHYQQISGLQQQPKLSQHHILPLVQGQSTVLPVHVLGPPVVSQPQVSPLTVQTVSQVKPISQAVGVEQQATLLKPDLVRSLNQDVAAVKENINTPDNLYGNGKQDRSKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDADGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHAHSAVDRAIGTDSIIVESNSSQTGSSEQVQINSASTQTSNESAPQSSPVGRWRFCINQTIRNREAQSPPLQQSMSTVPGLPLLSGPRTTSNKEISQDTLLTLENNPCQRVLFASKSELKDVVDGKISEYASVDTKQQAVFFQVEDDRQIMASVASSSTHTATSVRAVPVECEGLTNQAGIFLPAYPCHQAASQADVLMVPPGESTQIAGNSLTTLAFMSDQKPQSLAVQQPTMDAELISQEGETTVNTEASSPKMVIPTQTPGLELTTLLPSTVLESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVPEVIGISHSGIQDSPAQSPNFQQTGSKILSNVAVSQPANISVFKRDLNVITSIPSEICLHEMSPDASLPGDPETYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDMRSAVAPDPIPLTGESTADTRALSRCKVMSGSFQRGRFQVITVPQQQSVKVTSFGIEHIPAFNKMTTHSSEEVLAFTEIAKSQLVKMETSMCNPQTSLPSQKLRAHRETFKEDKRIAKQGDNYSSFNTACETDVSLMTPEKEPEENSAMGSSMHSGLELLLKERETFTAGKHPSSDSEFSATLAGRGRSVAKTAPVSDQCLPLREEKAFAQTQSSLFYSPSSPMSSDDDSEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRAIKDSKIQSSEAPLPPSSPRRPRSFKSKLRSRPQSWTHVDNVAVATDPLCVESNIASCQQSPASKKGMFTDDLHKLVDDWTKETVGNSLIKPSLNQLKQSQHKLETENWNKVYENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLTSFPGTLSSYGMPHVCQYNAVGGPGYPVQWVGISGTPQQSVVIPTQSGGPFQPGMNLQAFPASSVHNPSAIPPGPK

Target ORF information:

RefSeq Version	XM_011291755.3
Organism	Felis catus(domestic cat)
Definition	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011291755.3

ORF Insert Sequence:

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_019823858.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019679417.1 Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X2, mRNA.

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CloneID	OFb31125
Clone ID Related Accession (Same CDS sequence)	XM_011291755.2 , XM_019823859.1 , XM_019823860.2 , XM_011291755.3 , XM_019823858.1 , XM_019823860.1
Accession Version	XM_019823858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019679417.1
CDS	204..5588
Translation	MATDSGEPASTEDSEKPDGVSLEDRAPQVAATLTVEARLKEKNSTFSATGETTERKRFFRKSVEMMEDDKVAESSSKDERMKTATNIPRVEKLPTNVLGGGQEVKYEQCSKATSESSKDCLKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTSEEVAYEMVRSGFFHESDSKAVAKSIRDRVTLIKKTREKKPAGCLEERRDSQCKSVGNVLPQHQNTTLSPAPAQHTGAECEETEVDQHVRQQFLQRKPQQHCSSVTGDNLSEAGAGSAIHSDTSSHPSVAYSSDQKMGSQVVSNIPQAEINVPGMIYPSQQRVGHYQQISGLQQQPKLSQHHILPLVQGQSTVLPVHVLGPPVVSQPQVSPLTVQTVSQVKPISQAVGVEQQATLLKPDLVRSLNQDVAAVKENINTPDNLYGNGKQDRSKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDADGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHAHSAVDRAIGTDSIIVESNSSQTGSSEQVQINSASTQTSNESAPQSSPVGRWRFCINQTIRNREAQSPPLQQSMSTVPGLPLLSGPRTTSNKEISQDTLLTLENNPCQRVLFASKSELKDVVDGKISEYASVDTKQQAVFFQVEDDRQIMASVASSSTHTATSVRAVPVECEGLTNQAGIFLPAYPCHQAASQADVLMVPPGESTQIAGNSLTTLAFMSDQKPQSLAVQQPTMDAELISQEGETTVNTEASSPKMVIPTQTPGLELTTLLPSTVLESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVPEVIGISHSGIQDSPAQSPNFQQTGSKILSNVAVSQPANISVFKRDLNVITSIPSEICLHEMSPDASLPGDPETYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDMRSAVAPDPIPLTGESTADTRALSRCKVMSGSFQRGRFQVITVPQQQSVKVTSFGIEHIPAFNKMTTHSSEEVLAFTEIAKSQLVKMETSMCNPQTSLPSQKLRAHRETFKEDKRIAKQGDNYSSFNTACETDVSLMTPEKEPEENSAMGSSMHSGLELLLKERETFTAGKHPSSDSEFSATLAGRGRSVAKTAPVSDQCLPLREEKAFAQTQSSLFYSPSSPMSSDDDSEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRAIKDSKIQSSEAPLPPSSPRRPRSFKSKLRSRPQSWTHVDNVAVATDPLCVESNIASCQQSPASKKGMFTDDLHKLVDDWTKETVGNSLIKPSLNQLKQSQHKLETENWNKVYENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLTSFPGTLSSYGMPHVCQYNAVGGPGYPVQWVGISGTPQQSVVIPTQSGGPFQPGMNLQAFPASSVHNPSAIPPGPK

Target ORF information:

RefSeq Version	XM_019823858.1
Organism	Felis catus(domestic cat)
Definition	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019823858.1

ORF Insert Sequence:

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK3 ( XM_019823860.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019679419.1 Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.

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CloneID	OFb31125
Clone ID Related Accession (Same CDS sequence)	XM_011291755.2 , XM_019823859.1 , XM_019823860.2 , XM_011291755.3 , XM_019823858.1 , XM_019823860.1
Accession Version	XM_019823860.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	serine/threonine-protein kinase WNK3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019679419.1
CDS	202..5586
Translation	MATDSGEPASTEDSEKPDGVSLEDRAPQVAATLTVEARLKEKNSTFSATGETTERKRFFRKSVEMMEDDKVAESSSKDERMKTATNIPRVEKLPTNVLGGGQEVKYEQCSKATSESSKDCLKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTSEEVAYEMVRSGFFHESDSKAVAKSIRDRVTLIKKTREKKPAGCLEERRDSQCKSVGNVLPQHQNTTLSPAPAQHTGAECEETEVDQHVRQQFLQRKPQQHCSSVTGDNLSEAGAGSAIHSDTSSHPSVAYSSDQKMGSQVVSNIPQAEINVPGMIYPSQQRVGHYQQISGLQQQPKLSQHHILPLVQGQSTVLPVHVLGPPVVSQPQVSPLTVQTVSQVKPISQAVGVEQQATLLKPDLVRSLNQDVAAVKENINTPDNLYGNGKQDRSKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDADGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHAHSAVDRAIGTDSIIVESNSSQTGSSEQVQINSASTQTSNESAPQSSPVGRWRFCINQTIRNREAQSPPLQQSMSTVPGLPLLSGPRTTSNKEISQDTLLTLENNPCQRVLFASKSELKDVVDGKISEYASVDTKQQAVFFQVEDDRQIMASVASSSTHTATSVRAVPVECEGLTNQAGIFLPAYPCHQAASQADVLMVPPGESTQIAGNSLTTLAFMSDQKPQSLAVQQPTMDAELISQEGETTVNTEASSPKMVIPTQTPGLELTTLLPSTVLESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVPEVIGISHSGIQDSPAQSPNFQQTGSKILSNVAVSQPANISVFKRDLNVITSIPSEICLHEMSPDASLPGDPETYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDMRSAVAPDPIPLTGESTADTRALSRCKVMSGSFQRGRFQVITVPQQQSVKVTSFGIEHIPAFNKMTTHSSEEVLAFTEIAKSQLVKMETSMCNPQTSLPSQKLRAHRETFKEDKRIAKQGDNYSSFNTACETDVSLMTPEKEPEENSAMGSSMHSGLELLLKERETFTAGKHPSSDSEFSATLAGRGRSVAKTAPVSDQCLPLREEKAFAQTQSSLFYSPSSPMSSDDDSEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRAIKDSKIQSSEAPLPPSSPRRPRSFKSKLRSRPQSWTHVDNVAVATDPLCVESNIASCQQSPASKKGMFTDDLHKLVDDWTKETVGNSLIKPSLNQLKQSQHKLETENWNKVYENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLTSFPGTLSSYGMPHVCQYNAVGGPGYPVQWVGISGTPQQSVVIPTQSGGPFQPGMNLQAFPASSVHNPSAIPPGPK

Target ORF information:

RefSeq Version	XM_019823860.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus WNK lysine deficient protein kinase 3 (WNK3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019823860.1

ORF Insert Sequence:

ATGGCTACTG ATTCAGGAGA GCCAGCTAGC ACAGAAGATT CTGAGAAACC TGATGGAGTT 
TCATTGGAAG ACAGAGCTCC TCAGGTTGCA GCAACTTTGA CAGTGGAAGC TAGACTAAAA 
GAAAAAAACA GTACTTTCTC TGCTACTGGG GAAACTACAG AAAGGAAGAG GTTCTTCCGA 
AAGAGTGTTG AGATGATGGA AGATGATAAA GTTGCTGAAT CTTCCTCCAA AGATGAGAGA 
ATGAAGACTG CAACAAATAT TCCAAGAGTA GAAAAGCTTC CTACAAATGT GCTGGGAGGT 
GGACAAGAAG TTAAATATGA ACAGTGTTCA AAGGCAACTT CAGAGTCCTC AAAAGATTGT 
TTGAAGGAGA AAAATGAAAA GGAAATGGAA GAAGAAGCAG AAATGAAGGC TGTAGCTACT 
TCTCCTAGTG GCAGGTTCCT GAAATTTGAC ATTGAGCTGG GAAGAGGAGC ATTTAAAACA 
GTATATAAAG GATTGGACAC TGAAACATGG GTTGAGGTTG CTTGGTGTGA GCTGCAGGAC 
CGGAAATTAA CCAAAGCTGA GCAGCAAAGG TTCAAGGAAG AAGCAGAGAT GTTAAAAGGT 
CTCCAACATC CCAATATAGT TCGATTTTAT GATTCCTGGG AGTCTATATT AAAGGGAAAG 
AAATGTATTG TATTGGTGAC TGAACTTATG ACATCAGGAA CCTTAAAAAC GTACTTAAAA 
CGATTTAAAG TCATGAAACC AAAGGTCTTA AGGAGTTGGT GCAGGCAAAT TTTAAAGGGA 
TTGCAGTTCT TGCACACTAG GACTCCTCCC ATTATTCACC GGGATCTGAA GTGTGACAAT 
ATTTTCATCA CGGGACCCAC TGGATCTGTG AAGATTGGTG ATCTAGGACT AGCCACCTTA 
ATGCGAACAT CATTTGCTAA GAGTGTCATT GGGACTCCTG AGTTTATGGC CCCAGAAATG 
TATGAAGAGC ACTACGATGA ATCTGTAGAT GTCTATGCTT TTGGAATGTG TATGCTGGAA 
ATGGCTACTT CTGAATACCC TTATTCTGAG TGCCAGAATG CAGCTCAAAT ATACCGTAAA 
GTAACTAGCG GCATAAAGCC AGCCAGTTTC AATAAAGTCA CTGATCCTGA AGTGAAAGAA 
ATCATTGAAG GGTGTATTCG TCAAAACAAA TCAGAAAGGT TGTCTATCAG GGACCTATTA 
AACCACGCAT TTTTTGCTGA GGATACAGGA CTGAGGGTGG AGTTAGCAGA GGAAGATGAC 
TGCTCTAATT CGTCCCTGGC TTTAAGACTC TGGGTTGAAG ACCCTAAAAA ATTGAAAGGC 
AAACACAAAG ACAATGAAGC TATTGAATTC AGTTTCAATT TAGAAACGGA TACATCTGAG 
GAAGTAGCAT ATGAAATGGT CAGGTCAGGA TTCTTCCATG AAAGTGATTC CAAAGCTGTT 
GCTAAGTCCA TTAGAGACCG GGTCACCCTG ATAAAGAAGA CAAGGGAGAA GAAACCAGCT 
GGCTGTTTGG AAGAACGCAG GGATTCCCAG TGCAAGTCTG TGGGGAATGT ACTCCCTCAG 
CACCAGAATA CAACTTTGTC GCCTGCTCCT GCTCAGCACA CGGGGGCTGA ATGTGAAGAA 
ACTGAAGTTG ATCAGCATGT TCGACAACAG TTTCTACAAA GAAAACCACA GCAGCACTGC 
TCCTCTGTTA CAGGTGACAA TTTGTCTGAG GCAGGAGCTG GATCTGCTAT ACACTCAGAT 
ACTTCAAGTC ATCCCAGTGT GGCCTATTCC TCAGACCAGA AGATGGGTTC TCAAGTGGTT 
TCCAACATCC CACAGGCTGA AATAAATGTT CCAGGGATGA TTTATCCATC CCAGCAGCGA 
GTAGGACATT ACCAGCAGAT TTCAGGGCTG CAACAGCAGC CAAAGCTGTC TCAGCACCAC 
ATTTTGCCTT TGGTTCAAGG TCAGTCCACT GTTTTGCCTG TACATGTCCT TGGACCTCCA 
GTTGTCTCAC AACCCCAGGT TTCCCCATTA ACTGTCCAGA CGGTCTCACA GGTAAAGCCT 
ATATCCCAGG CAGTTGGAGT TGAACAACAA GCAACTCTTC TAAAACCAGA TTTAGTTAGA 
AGTCTGAATC AAGATGTGGC AGCTGTGAAG GAAAACATCA ATACTCCTGA TAACCTATAT 
GGAAATGGCA AACAAGATCG GAGCAAACAA AGAAGAGCTT CCTGTCCTCG ACCAGAGAAG 
GGGACTAAAT TCCAACTTAC TGTGCTTCAG GTGTCAACAT CTGGAGACAA CATGGTGGAG 
TGTCAGTTGG AGACACACAA CAACAAGATG GTCACCTTCA AGTTTGATGC TGATGGTGAC 
GCACCAGAAG ACATTGCAGA TTATATGGTT GAAGATAACT TTGTGCTGGA AAGCGAGAAA 
GAAAAATTTG TAGAAGAATT GAGGGCTATT GTAGGTCAGG CCCAGGAGAT CCTTCATGCC 
CACTCTGCTG TAGATAGAGC CATTGGAACT GACTCTATAA TTGTGGAATC CAACAGTAGC 
CAAACAGGAT CATCTGAACA AGTACAGATA AATTCTGCAT CCACTCAGAC CAGCAATGAA 
TCTGCTCCTC AGTCATCTCC AGTTGGTCGG TGGCGATTCT GTATCAATCA AACAATAAGA 
AACCGTGAGG CTCAGTCTCC TCCTCTTCAG CAGTCCATGT CTACAGTTCC TGGGTTACCT 
CTACTTTCTG GTCCAAGAAC CACAAGTAAT AAGGAAATAT CACAGGATAC ACTGCTCACT 
CTAGAAAATA ATCCCTGCCA GCGTGTACTT TTTGCCTCCA AATCAGAACT CAAGGATGTA 
GTTGATGGTA AGATTTCTGA ATATGCCAGT GTAGACACTA AGCAGCAAGC TGTATTTTTT 
CAAGTGGAAG ATGACAGGCA GATAATGGCA TCAGTTGCTA GTAGTTCAAC TCATACTGCT 
ACTTCAGTTC GTGCAGTTCC AGTTGAATGT GAGGGACTCA CCAACCAAGC AGGCATATTT 
CTACCTGCAT ATCCATGTCA CCAAGCTGCC AGTCAGGCTG ATGTACTTAT GGTCCCTCCT 
GGCGAGTCAA CTCAGATTGC TGGTAACTCT CTTACAACTC TGGCATTTAT GTCTGATCAA 
AAGCCTCAAT CCTTAGCAGT GCAGCAACCA ACTATGGATG CAGAGTTGAT TTCCCAAGAA 
GGAGAAACCA CAGTGAACAC TGAAGCAAGT TCTCCTAAGA TGGTCATTCC CACTCAGACC 
CCTGGCCTTG AACTGACTAC CCTTCTACCC TCTACTGTCC TGGAATCAGA TGGGGAAAGA 
CCTCCAAAAA TGGAGTTTGC AGACAACCGA ATTAAAACTC TGGATGAAAA ATTAAGAAAC 
TTGCTCTATC AGGAGCATAG CATATCTAGC ATCTATCCTG AGAGTCAGAA GGATACCCAA 
AGCATAGACT CTCCATTTTC TTCCTCTGCT GAAGATACGC TCTCATGTCC AGTGCCAGAA 
GTCATAGGCA TCAGTCACAG TGGAATTCAA GATAGTCCTG CACAGTCCCC TAATTTTCAG 
CAGACAGGCT CTAAGATTCT GTCCAATGTG GCTGTGAGTC AGCCTGCTAA CATATCAGTG 
TTCAAAAGGG ACCTGAATGT GATAACTTCT ATACCCAGCG AAATATGTTT ACATGAGATG 
TCTCCAGATG CTTCACTTCC AGGGGATCCA GAGACCTATC CTGCTGCTGT GTCAAGTGGT 
GGAGCCATTC ATCTGCAGAC AGGAGGTGGA TATTTTGGCC TAAGCTTTAC TTGTCCTAGT 
CTCAAAAATC CTATTAGCAA GAAATCCTGG ACTCGCAAAT TAAAAAGCTG GGCATACAGG 
CTACGGCAGT CAACCAGCTT TTTCAAGAGA TCAAAAGTCC GTCAAGTGGA AACAGAAGAT 
ATGAGATCAG CAGTTGCTCC TGATCCCATT CCTCTGACAG GAGAGTCCAC AGCTGATACT 
AGGGCTTTGA GTAGATGCAA AGTGATGAGT GGATCATTTC AGCGGGGTCG GTTCCAGGTG 
ATTACAGTTC CTCAGCAGCA GTCAGTGAAA GTGACATCTT TTGGAATAGA ACACATACCA 
GCATTCAATA AAATGACAAC CCATTCTAGT GAAGAAGTTC TAGCCTTTAC TGAAATAGCC 
AAGTCTCAGT TAGTGAAAAT GGAAACTTCC ATGTGCAATC CACAAACTTC ACTTCCTTCT 
CAGAAGTTAC GAGCTCATCG TGAAACCTTT AAAGAAGATA AAAGAATTGC CAAACAAGGT 
GACAACTACT CATCTTTCAA CACAGCTTGT GAGACTGATG TATCTTTGAT GACCCCAGAA 
AAGGAACCGG AAGAAAACTC AGCCATGGGA AGTAGTATGC ATTCTGGACT TGAACTATTG 
CTTAAAGAGA GAGAGACATT TACTGCTGGG AAACATCCTA GCTCTGATAG TGAATTTTCA 
GCCACTCTTG CTGGCAGAGG GAGGTCAGTG GCAAAGACTG CTCCAGTGAG TGATCAGTGC 
TTACCACTCC GTGAAGAAAA AGCCTTTGCT CAAACACAGA GCTCACTCTT CTATTCACCA 
TCTTCTCCAA TGAGCAGTGA TGATGACTCA GAAATAGAGG ATGAGGATTT GAAGGTGGAG 
CTTCAAAGAT TACGAGAAAA ACACATTCAG GAGGTGGTAA ATCTTCAAAC CCAGCAGAAT 
AAGGAGCTGC AAGAGCTCTA TGAACGCCTT CGGGCAATTA AAGATAGCAA AATCCAATCA 
TCAGAGGCTC CTCTGCCACC ATCATCACCA CGTAGACCAA GATCTTTCAA AAGCAAACTT 
CGCAGCCGCC CCCAGTCCTG GACACATGTG GACAATGTCG CAGTTGCCAC AGATCCGCTC 
TGTGTGGAGA GTAATATAGC ATCATGCCAG CAATCTCCAG CTAGTAAAAA AGGCATGTTC 
ACAGATGACT TACATAAACT GGTGGATGAC TGGACAAAGG AAACAGTTGG AAATTCTCTT 
ATTAAGCCAA GTTTAAACCA ACTCAAACAG AGTCAACACA AACTAGAGAC AGAAAACTGG 
AACAAAGTAT ATGAAAATAC TCCATCTACT ATGGGCTACA CATCAACATG GATTTCTTCT 
CTGTCCCAAA TCCGTGGAGC TGTCCCAACC TCCTTGCCAC AAGGACTCTC ACTCACTTCA 
TTTCCTGGGA CATTATCATC ATATGGAATG CCCCATGTCT GTCAGTATAA TGCTGTGGGG 
GGGCCAGGAT ACCCAGTACA ATGGGTAGGA ATTTCAGGAA CACCACAACA GTCTGTAGTA 
ATTCCGACTC AATCTGGGGG ACCATTCCAG CCGGGAATGA ATTTACAGGC ATTTCCAGCT 
TCATCAGTGC ATAATCCATC TGCCATCCCG CCTGGTCCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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WNK3 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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