OTOF gene cDNA ORF clone, Felis catus(domestic cat)

mRNA	Protein	Name
XM_023252024.1	XP_023107792.1	otoferlin isoform X4
XM_019827790.2	XP_019683349.1	otoferlin isoform X1
XM_023252023.1	XP_023107791.1	otoferlin isoform X3
XM_019827789.1	XP_019683348.1	otoferlin isoform X1
XM_019827790.1	XP_019683349.1	otoferlin isoform X2
XM_019827793.1	XP_019683352.1	otoferlin isoform X4
XM_019827795.1	XP_019683354.1	otoferlin isoform X6
XM_019827792.1	XP_019683351.1	otoferlin isoform X3
XM_019827794.1	XP_019683353.1	otoferlin isoform X5
XM_019827793.2	XP_019683352.1	otoferlin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb40725	XM_019827790.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb40726	XM_019827792.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb40727	XM_019827793.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb40728	XM_019827794.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb40729	XM_019827795.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb73951	XM_019827790.2 Latest version!	Felis catus otoferlin (OTOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb73952	XM_019827793.2 Latest version!	Felis catus otoferlin (OTOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb73953	XM_023252023.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb73954	XM_023252024.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb40724	XM_019827789.1 Latest version!	Felis catus otoferlin (OTOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

OTOF ( XM_019827790.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683349.1 Felis catus otoferlin (OTOF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb40725
Clone ID Related Accession (Same CDS sequence)	XM_019827790.1
Accession Version	XM_019827790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	otoferlin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGCAGGCT CCCTCGGGGT CGCCGTGTGC TGTGCGATGG CGGTCCCTCC 
TCTCACCCCC CAGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCGC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683349.1
CDS	1..5997
Translation	MALFVHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENREDVADFDETFRWPVASSIDSNEILEIQIFNYSKVFSNKLIGTFRMVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGRLPRGRRVLCDGGPSSHPPGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827790.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827790.1

ORF Insert Sequence:

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGCAGGCT CCCTCGGGGT CGCCGTGTGC TGTGCGATGG CGGTCCCTCC 
TCTCACCCCC CAGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCGC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_019827792.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683351.1 Felis catus otoferlin (OTOF), transcript variant X3, mRNA.

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CloneID	OFb40726
Clone ID Related Accession (Same CDS sequence)	XM_019827792.1
Accession Version	XM_019827792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	otoferlin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC ACAAAGCTGG GAGAAGTGTG TTCTCCGCCA TGAAGCTCGG CAAGAACCGG 
TCCCACAAGG AGGAACCCCA AAGACAAGAT GAACCGGCCG TGCTGGAGAT GGAGGACCTT 
GACCGCTTGG CCGTTCGGCT GGGGGATGGG CTGGACCCTG ACTCTGTGTC TCTGGCCTCT 
GTCACAGCTC TCACCACCAA TGTCTCCAAC AAACGATCCA AGCCAGACAT TAAGATGGTG 
CCAGGTGCCG GGCGGCCCGT GGATTACCAG GTCAGCATCA CCGTGATTGA GGCTCGGCAG 
CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGCGACGA CAAGAAGTAC 
ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTCGT CTTCGACTTC 
CACGTCTCTC CGGACGTCAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG 
AACCTGCTTC GAAGCGGTAC CCTGGTGGGG TCCTTCAAGA TGGATGTGGG AACTGTGTAC 
TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCGGACCC CGACGACATC 
TCCGCCGGGC TGAAGGGCTA CGTGAAGTGT GACGTGGCCG TGGTGGGCAA GGGGGACAAC 
ATCAAGACGC CCCACAAGGC CAACGAGACA GACGAGGATG ACATTGAAGG GAACTTGCTG 
CTCCCCGAGG GGGTGCCCCC CGAACGCCAG TGGGCCCGGT TCTACGTGAA GATTTACCGA 
GCAGAAGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCGTTCATC 
GGGGAGAACA AGGACCTGGT TGACCCCTAC GTGCAAGTCT TCTTTGCCGG CCAGAAGGGC 
AAGACATCAG TACAGAAAAG CAGCTACGAG CCACTGTGGA ATGAGCAGGT CGTCTTTACG 
GACCTGTTCC CCCCCCTCTG CAAGCGCATG AAGGTGCAGA TCCGAGATGC CGACAAGGTC 
AACGACGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCTAA TGATGGAGAC 
AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC TACACGCAAC 
TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGGGAGGG CGTGTCGTTC 
CGCGCCCGCC TCATGCTGGG CCTGGCGGTG GAGATCCTGG ACACCTCCAA TCCTGAGCTG 
ACCAGCTCCA CGGAGGTGCA GGTGGAGCAG GCCACGCCTG TCTCGGAGAG CTGCACGGGG 
AAAATGGAAG AATTCTTTCT ATTCGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA 
AATGGCGACA AGCCAATCAC CTTTGAGGTG ACCATAGGCA ACTATGGGAA TGAAGTTGAT 
GGCCTGTCGC GGCCCCAGCG GCCTAGGCCC CGGAAGGAGC CTGGGGATGA GGAAGAAGTA 
GATCTGATCC AGAACTCCAG TGATGAAGAT GCCGGTGAGG GTGGGGACCT GGCCTCGGTG 
TCCTCGACTC CGCCCATGCG GCCCCAGATC ACCGACAGGA ACTACTTCCA TCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATCAAG AGCTGGTGGC CCGACCAGCG TCGCCGCCTC 
TACAACGCCA ACATCATGGA CCACATCGCG GACAAGCTGG AAGAAGGCCT GAACGACGTG 
CAGGAGATGA TAAAGACAGA GAAGTCCTAT CCGGAGCGTC GCCTGCGGGG TGTCCTGGAG 
GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCTG ACAAGGACCA GGGCCACTCG 
TCCCGCACCA GGCTGGACAG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAGAGTATG 
GGGCAGCAGG CCAAGACCTT GCGGGCACAG GTGAAGCGGC ACACGGTGCG GGACAAGCTG 
AGGCTGTGCC AGAATTTCCT GCAGAAACTG CGCTTCCTGG CGGACGAGCC TCAGCACAGC 
ATCCCCGACG TCTTCATCTG GATGATGAAC AACAACAAGC GCACCGCCTA CGCCAGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGCAAGGA CTGCGCCAAG 
GTCAAGACGC TCTTCCTCAA GCTGCCGGGC AAGCGGGGCT TCGGCTCCGT GGGCTGGACG 
GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTGA GCAAGCAGCG CAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC 
GCCTTCCCGC CCATCAGCCT GGTCTACACC AAGAAGCAGG CGTTCCAGCT CCGGGCCCAC 
ATGTACCAGG CTCGCAGCCT CTTCGCTGCC GACAGCAGCG GCCTCTCGGA CCCCTTCGCC 
CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACGGAGGTGC TGAATGAGAC CCTGTGCCCC 
ACCTGGGACC AGATGCTGGT GTTCGACAAC CTGGAGCTAT ATGGCGAAGC CCATGAGTTG 
CGGGATGACC CCCCCATCAT CGTCATCGAA ATCTACGACC AGGACACCAT GGGCAAAGCC 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCGGACGA GGCGTACTGT 
CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA CGCCACTGCC 
GGGGACCTGC TGGCTGCCTT CGAGTTGCTG CAGATTGGGC CTGCGGGGAA GGCAGACCTG 
CCACCCATCA ACGGCCCGGG GGACATGGAC CGAGGTCCCA TCATGCCTGT GCCCGTCGGC 
ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GCGGGACCTG 
AAGCGGGTCA ACCTGGCCCA GGTGGACCGG CCGCGGGTAG ACATCGAGTG TGCCGGGAAG 
GGGGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC 
AAGTGGTTTG AAGTGGACCT CCCGGAGAAC GAGCTGCTGC ACCCGCCCCT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GTTCCCACGC CGTCAGCTCT 
CTGAGGCGCT TCATCTACAG GCCCCCAGAC CGCTCGGCGC CCAACTGGAA CACCACGGGG 
GAGGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCCGGA AGCTGGAGAC CATGGTGAAG 
CTGGACGCGA CTTCTGATGC CGTCATCAAG GTGGATGTGG CTGACGAGGA GAAAGAGAAG 
AAGAAAAAGA AGAAAAAGGG CAGCTCAGAA GAGCCAGAGG AGGAGGAGCC CGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGATA CCATGAAGGA GCAACTTCGA 
CAGCAAGAGG CCTCCGGCAT TGACTTGGAG GAGAAGGAGG AGGTGGAGAA CACGGAGGGC 
CTAAAGGGGC CGATGAAGGG CAAGGAGAAG GCAAAGGCGG CGAAGGAGGA GAAGAAAAAG 
AGAACCCAGA GCCCCGGCAC TGGCCAGGGG CCTGAGGCCC CAGAGAAGAA GAAACCCAAG 
ATTGATGAGC TTAAGGTGTA CCCCAAGGAG CTGGAGTCTG AGTTTGACAA CTTTGAGGAC 
TGGCTGCACA CTTTCAATCT GCTGCGGGGC AAGACGGGGG ATGACGAGGA CGGTTCCACG 
GAGGAGGAGC GCATCGTGGG CCGGTTCAAG GGCTCCCTCT GCGTGTATAA AGTGCCACTC 
CCAGAGGACG TGTCCCGGGA AGCCGGCTAC GACCCCACCT ACGGCATGTT CCAGGGCATC 
CCCAGCAACG ACCCCATCAA TGTGCTGGTC CGAGTCTATG TGGTCAGGGC CACGGACCTG 
CACCCCGCAG ACATTAACGG CAAAGCCGAC CCCTACATTG CCATCCGGCT GGGCAAAACT 
GACATCCGAG ACAAGGAGAA CTACATCTCC AAGCAGCTCA ACCCTGTCTT TGGGAAGTCC 
TTTGACATCG AGGCCTCCTT CCCCATGGAA TCCATGCTGA CAGTGGCCGT GTACGACTGG 
GATCTGGTAG GCACCGATGA CCTCATCGGG GAAACCAAGA TTGACCTGGA GAACCGCTTC 
TACAGCAAAC ACCGGGCCAC CTGTGGCATC GCCCAGACCT ACTCCATACA TGGCTACAAT 
ATCTGGCGGG ACCCCATGAA GCCCAGCCAG ATTCTGACCC GCCTCTGCAA GGAGGGCAAA 
GTGGACGGCC CCCACTTTGG GCCCCCCGGG AGAGTGAAGG TGTCCAACCG TGTCTTCACT 
GGGCCCTCCG AGATCGAGGA CGAGAATGGT CAGAGGAAGC CTACGGAAGA GCATGTGGCT 
CTATCTGCCC TGAGGCACTG GGAGGACATC CCCCGCGTGG GCTGCCGCCT GGTGCCCGAA 
CACGTGGAAA CCCGGCCCCT GCTCAACCCT GACAAGCCAG GCATCGAGCA GGGCCGCCTG 
GAGCTGTGGG TGGACATGTT CCCCATGGAC ATGCCGGCCC CTGGGACGCC TCTGGACATC 
TCCCCCCGGA AGCCCAAGAA GTATGAGCTC CGGGTCATCG TGTGGAACAC AGACGAGGTG 
GTCCTGGAGG ACGATGACTT CTTTACTGGG GAGAAGTCCA GCGACATTTT TGTGCGGGGG 
TGGCTGAAGG GCCAGCAGGA GGACAAGCAG GACACGGACG TCCACTATCA TTCCCTCACC 
GGCGAGGGCA ACTTCAACTG GCGCTACCTG TTCCCCTTCG ACTACCTGGC CGCCGAGGAA 
AGGATTGTCA TCTCCAAGAA GGAGTCCATG TTCTCCTGGG ACGAGACCGA GTACAAGATC 
CCCGCGCGGC TCACCCTGCA GATCTGGGAC GCGGACCACT TCTCCGCGGA CGACTTCCTG 
GGGGCCATCG AGCTGGACCT CAACCGCTTT CCCCGGGGCG CCAAGACAGC CAAGCAGTGC 
ACCATGGAGA TGGCCACGGG GGAGGTGGAC GTGCCCCTGG TTTCCATCTT CAAGCAGAAG 
CGCGTCAAAG GCTGGTGGCC CCTCCTGGCC CGCAATGAGA ATGATGAGTT CGAGCTCACG 
GGCAAGGTGG AGGCAGAATT GCATCTACTG ACAGCGGAGG AGGCAGAGAA GAATCCGGTG 
GGCCTGGCCC GCAATGAACC TGACCCTCTA GAGAAACCCA ACCGGCCTGA CACCGCCTTC 
GTCTGGTTCC TCAACCCCCT CAAGTCCATC AAGTACCTCA TCTGCACGCG GTACAAGTGG 
CTCATCATCA AGATCGTGCT GGCGCTGCTG GGGCTGCTCA TGCTGGCCCT CTTCCTCTAC 
AGCCTCCCCG GCTACATGGT CAAGAAGCTC CTGGGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683351.1
CDS	1..5982
Translation	MALFVHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENREDVADFDETFRWPVASSIDSNEILEIQIFNYSKVFSNKLIGTFRMVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRSKGREKTKGGRDGEHKAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827792.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827792.1

ORF Insert Sequence:

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC ACAAAGCTGG GAGAAGTGTG TTCTCCGCCA TGAAGCTCGG CAAGAACCGG 
TCCCACAAGG AGGAACCCCA AAGACAAGAT GAACCGGCCG TGCTGGAGAT GGAGGACCTT 
GACCGCTTGG CCGTTCGGCT GGGGGATGGG CTGGACCCTG ACTCTGTGTC TCTGGCCTCT 
GTCACAGCTC TCACCACCAA TGTCTCCAAC AAACGATCCA AGCCAGACAT TAAGATGGTG 
CCAGGTGCCG GGCGGCCCGT GGATTACCAG GTCAGCATCA CCGTGATTGA GGCTCGGCAG 
CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGCGACGA CAAGAAGTAC 
ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTCGT CTTCGACTTC 
CACGTCTCTC CGGACGTCAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG 
AACCTGCTTC GAAGCGGTAC CCTGGTGGGG TCCTTCAAGA TGGATGTGGG AACTGTGTAC 
TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCGGACCC CGACGACATC 
TCCGCCGGGC TGAAGGGCTA CGTGAAGTGT GACGTGGCCG TGGTGGGCAA GGGGGACAAC 
ATCAAGACGC CCCACAAGGC CAACGAGACA GACGAGGATG ACATTGAAGG GAACTTGCTG 
CTCCCCGAGG GGGTGCCCCC CGAACGCCAG TGGGCCCGGT TCTACGTGAA GATTTACCGA 
GCAGAAGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCGTTCATC 
GGGGAGAACA AGGACCTGGT TGACCCCTAC GTGCAAGTCT TCTTTGCCGG CCAGAAGGGC 
AAGACATCAG TACAGAAAAG CAGCTACGAG CCACTGTGGA ATGAGCAGGT CGTCTTTACG 
GACCTGTTCC CCCCCCTCTG CAAGCGCATG AAGGTGCAGA TCCGAGATGC CGACAAGGTC 
AACGACGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCTAA TGATGGAGAC 
AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC TACACGCAAC 
TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGGGAGGG CGTGTCGTTC 
CGCGCCCGCC TCATGCTGGG CCTGGCGGTG GAGATCCTGG ACACCTCCAA TCCTGAGCTG 
ACCAGCTCCA CGGAGGTGCA GGTGGAGCAG GCCACGCCTG TCTCGGAGAG CTGCACGGGG 
AAAATGGAAG AATTCTTTCT ATTCGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA 
AATGGCGACA AGCCAATCAC CTTTGAGGTG ACCATAGGCA ACTATGGGAA TGAAGTTGAT 
GGCCTGTCGC GGCCCCAGCG GCCTAGGCCC CGGAAGGAGC CTGGGGATGA GGAAGAAGTA 
GATCTGATCC AGAACTCCAG TGATGAAGAT GCCGGTGAGG GTGGGGACCT GGCCTCGGTG 
TCCTCGACTC CGCCCATGCG GCCCCAGATC ACCGACAGGA ACTACTTCCA TCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATCAAG AGCTGGTGGC CCGACCAGCG TCGCCGCCTC 
TACAACGCCA ACATCATGGA CCACATCGCG GACAAGCTGG AAGAAGGCCT GAACGACGTG 
CAGGAGATGA TAAAGACAGA GAAGTCCTAT CCGGAGCGTC GCCTGCGGGG TGTCCTGGAG 
GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCTG ACAAGGACCA GGGCCACTCG 
TCCCGCACCA GGCTGGACAG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAGAGTATG 
GGGCAGCAGG CCAAGACCTT GCGGGCACAG GTGAAGCGGC ACACGGTGCG GGACAAGCTG 
AGGCTGTGCC AGAATTTCCT GCAGAAACTG CGCTTCCTGG CGGACGAGCC TCAGCACAGC 
ATCCCCGACG TCTTCATCTG GATGATGAAC AACAACAAGC GCACCGCCTA CGCCAGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGCAAGGA CTGCGCCAAG 
GTCAAGACGC TCTTCCTCAA GCTGCCGGGC AAGCGGGGCT TCGGCTCCGT GGGCTGGACG 
GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTGA GCAAGCAGCG CAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC 
GCCTTCCCGC CCATCAGCCT GGTCTACACC AAGAAGCAGG CGTTCCAGCT CCGGGCCCAC 
ATGTACCAGG CTCGCAGCCT CTTCGCTGCC GACAGCAGCG GCCTCTCGGA CCCCTTCGCC 
CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACGGAGGTGC TGAATGAGAC CCTGTGCCCC 
ACCTGGGACC AGATGCTGGT GTTCGACAAC CTGGAGCTAT ATGGCGAAGC CCATGAGTTG 
CGGGATGACC CCCCCATCAT CGTCATCGAA ATCTACGACC AGGACACCAT GGGCAAAGCC 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCGGACGA GGCGTACTGT 
CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA CGCCACTGCC 
GGGGACCTGC TGGCTGCCTT CGAGTTGCTG CAGATTGGGC CTGCGGGGAA GGCAGACCTG 
CCACCCATCA ACGGCCCGGG GGACATGGAC CGAGGTCCCA TCATGCCTGT GCCCGTCGGC 
ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GCGGGACCTG 
AAGCGGGTCA ACCTGGCCCA GGTGGACCGG CCGCGGGTAG ACATCGAGTG TGCCGGGAAG 
GGGGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC 
AAGTGGTTTG AAGTGGACCT CCCGGAGAAC GAGCTGCTGC ACCCGCCCCT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GTTCCCACGC CGTCAGCTCT 
CTGAGGCGCT TCATCTACAG GCCCCCAGAC CGCTCGGCGC CCAACTGGAA CACCACGGGG 
GAGGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCCGGA AGCTGGAGAC CATGGTGAAG 
CTGGACGCGA CTTCTGATGC CGTCATCAAG GTGGATGTGG CTGACGAGGA GAAAGAGAAG 
AAGAAAAAGA AGAAAAAGGG CAGCTCAGAA GAGCCAGAGG AGGAGGAGCC CGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGATA CCATGAAGGA GCAACTTCGA 
CAGCAAGAGG CCTCCGGCAT TGACTTGGAG GAGAAGGAGG AGGTGGAGAA CACGGAGGGC 
CTAAAGGGGC CGATGAAGGG CAAGGAGAAG GCAAAGGCGG CGAAGGAGGA GAAGAAAAAG 
AGAACCCAGA GCCCCGGCAC TGGCCAGGGG CCTGAGGCCC CAGAGAAGAA GAAACCCAAG 
ATTGATGAGC TTAAGGTGTA CCCCAAGGAG CTGGAGTCTG AGTTTGACAA CTTTGAGGAC 
TGGCTGCACA CTTTCAATCT GCTGCGGGGC AAGACGGGGG ATGACGAGGA CGGTTCCACG 
GAGGAGGAGC GCATCGTGGG CCGGTTCAAG GGCTCCCTCT GCGTGTATAA AGTGCCACTC 
CCAGAGGACG TGTCCCGGGA AGCCGGCTAC GACCCCACCT ACGGCATGTT CCAGGGCATC 
CCCAGCAACG ACCCCATCAA TGTGCTGGTC CGAGTCTATG TGGTCAGGGC CACGGACCTG 
CACCCCGCAG ACATTAACGG CAAAGCCGAC CCCTACATTG CCATCCGGCT GGGCAAAACT 
GACATCCGAG ACAAGGAGAA CTACATCTCC AAGCAGCTCA ACCCTGTCTT TGGGAAGTCC 
TTTGACATCG AGGCCTCCTT CCCCATGGAA TCCATGCTGA CAGTGGCCGT GTACGACTGG 
GATCTGGTAG GCACCGATGA CCTCATCGGG GAAACCAAGA TTGACCTGGA GAACCGCTTC 
TACAGCAAAC ACCGGGCCAC CTGTGGCATC GCCCAGACCT ACTCCATACA TGGCTACAAT 
ATCTGGCGGG ACCCCATGAA GCCCAGCCAG ATTCTGACCC GCCTCTGCAA GGAGGGCAAA 
GTGGACGGCC CCCACTTTGG GCCCCCCGGG AGAGTGAAGG TGTCCAACCG TGTCTTCACT 
GGGCCCTCCG AGATCGAGGA CGAGAATGGT CAGAGGAAGC CTACGGAAGA GCATGTGGCT 
CTATCTGCCC TGAGGCACTG GGAGGACATC CCCCGCGTGG GCTGCCGCCT GGTGCCCGAA 
CACGTGGAAA CCCGGCCCCT GCTCAACCCT GACAAGCCAG GCATCGAGCA GGGCCGCCTG 
GAGCTGTGGG TGGACATGTT CCCCATGGAC ATGCCGGCCC CTGGGACGCC TCTGGACATC 
TCCCCCCGGA AGCCCAAGAA GTATGAGCTC CGGGTCATCG TGTGGAACAC AGACGAGGTG 
GTCCTGGAGG ACGATGACTT CTTTACTGGG GAGAAGTCCA GCGACATTTT TGTGCGGGGG 
TGGCTGAAGG GCCAGCAGGA GGACAAGCAG GACACGGACG TCCACTATCA TTCCCTCACC 
GGCGAGGGCA ACTTCAACTG GCGCTACCTG TTCCCCTTCG ACTACCTGGC CGCCGAGGAA 
AGGATTGTCA TCTCCAAGAA GGAGTCCATG TTCTCCTGGG ACGAGACCGA GTACAAGATC 
CCCGCGCGGC TCACCCTGCA GATCTGGGAC GCGGACCACT TCTCCGCGGA CGACTTCCTG 
GGGGCCATCG AGCTGGACCT CAACCGCTTT CCCCGGGGCG CCAAGACAGC CAAGCAGTGC 
ACCATGGAGA TGGCCACGGG GGAGGTGGAC GTGCCCCTGG TTTCCATCTT CAAGCAGAAG 
CGCGTCAAAG GCTGGTGGCC CCTCCTGGCC CGCAATGAGA ATGATGAGTT CGAGCTCACG 
GGCAAGGTGG AGGCAGAATT GCATCTACTG ACAGCGGAGG AGGCAGAGAA GAATCCGGTG 
GGCCTGGCCC GCAATGAACC TGACCCTCTA GAGAAACCCA ACCGGCCTGA CACCGCCTTC 
GTCTGGTTCC TCAACCCCCT CAAGTCCATC AAGTACCTCA TCTGCACGCG GTACAAGTGG 
CTCATCATCA AGATCGTGCT GGCGCTGCTG GGGCTGCTCA TGCTGGCCCT CTTCCTCTAC 
AGCCTCCCCG GCTACATGGT CAAGAAGCTC CTGGGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_019827793.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683352.1 Felis catus otoferlin (OTOF), transcript variant X4, mRNA.

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CloneID	OFb40727
Clone ID Related Accession (Same CDS sequence)	XM_019827793.1
Accession Version	XM_019827793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	otoferlin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCGC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683352.1
CDS	1..5937
Translation	MALFVHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENREDVADFDETFRWPVASSIDSNEILEIQIFNYSKVFSNKLIGTFRMVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827793.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827793.1

ORF Insert Sequence:

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCGC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_019827794.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683353.1 Felis catus otoferlin (OTOF), transcript variant X5, mRNA.

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CloneID	OFb40728
Clone ID Related Accession (Same CDS sequence)	XM_019827794.1
Accession Version	XM_019827794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	otoferlin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGC AGAAGGTGGT GGAGGAGAAG CGTGTGGAGG TGACCGACAC ACTGATCGAT 
GACAACAACG CTATCATCAA GACCAGCCTG TGCGTGGAGG TCCGATATCA GGCCACAGAT 
GGCACGGTGG GCTCCTGGGA TGATGGAGAC TTCCTGGGGG ATGAGTCCCT TCATGAGGAA 
GAGAAGGACA GCCAGGAGAC GGATGGACTG CTCCCCGGCT CCCGCCCCAG CTCCCGGACA 
CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAGAGATGGC 
GAGCACAAAG CTGGGAGAAG TGTGTTCTCC GCCATGAAGC TCGGCAAGAA CCGGTCCCAC 
AAGGAGGAAC CCCAAAGACA AGATGAACCG GCCGTGCTGG AGATGGAGGA CCTTGACCGC 
TTGGCCGTTC GGCTGGGGGA TGGGCTGGAC CCTGACTCTG TGTCTCTGGC CTCTGTCACA 
GCTCTCACCA CCAATGTCTC CAACAAACGA TCCAAGCCAG ACATTAAGAT GGTGCCAGGT 
GCCGGGCGGC CCGTGGATTA CCAGGTCAGC ATCACCGTGA TTGAGGCTCG GCAGCTCGTG 
GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGCG ACGACAAGAA GTACACATCC 
ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TCGTCTTCGA CTTCCACGTC 
TCTCCGGACG TCATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG 
CTTCGAAGCG GTACCCTGGT GGGGTCCTTC AAGATGGATG TGGGAACTGT GTACTCCCAG 
CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCGG ACCCCGACGA CATCTCCGCC 
GGGCTGAAGG GCTACGTGAA GTGTGACGTG GCCGTGGTGG GCAAGGGGGA CAACATCAAG 
ACGCCCCACA AGGCCAACGA GACAGACGAG GATGACATTG AAGGGAACTT GCTGCTCCCC 
GAGGGGGTGC CCCCCGAACG CCAGTGGGCC CGGTTCTACG TGAAGATTTA CCGAGCAGAA 
GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCGTT CATCGGGGAG 
AACAAGGACC TGGTTGACCC CTACGTGCAA GTCTTCTTTG CCGGCCAGAA GGGCAAGACA 
TCAGTACAGA AAAGCAGCTA CGAGCCACTG TGGAATGAGC AGGTCGTCTT TACGGACCTG 
TTCCCCCCCC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ATGCCGACAA GGTCAACGAC 
GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CTAATGATGG AGACAAAGGC 
TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCTACACG CAACTACACG 
CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGGG AGGGCGTGTC GTTCCGCGCC 
CGCCTCATGC TGGGCCTGGC GGTGGAGATC CTGGACACCT CCAATCCTGA GCTGACCAGC 
TCCACGGAGG TGCAGGTGGA GCAGGCCACG CCTGTCTCGG AGAGCTGCAC GGGGAAAATG 
GAAGAATTCT TTCTATTCGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAATGGC 
GACAAGCCAA TCACCTTTGA GGTGACCATA GGCAACTATG GGAATGAAGT TGATGGCCTG 
TCGCGGCCCC AGCGGCCTAG GCCCCGGAAG GAGCCTGGGG ATGAGGAAGA AGTAGATCTG 
ATCCAGAACT CCAGTGATGA AGATGCCGGT GAGGGTGGGG ACCTGGCCTC GGTGTCCTCG 
ACTCCGCCCA TGCGGCCCCA GATCACCGAC AGGAACTACT TCCATCTGCC CTACCTGGAG 
CGCAAGCCCT GCATCTACAT CAAGAGCTGG TGGCCCGACC AGCGTCGCCG CCTCTACAAC 
GCCAACATCA TGGACCACAT CGCGGACAAG CTGGAAGAAG GCCTGAACGA CGTGCAGGAG 
ATGATAAAGA CAGAGAAGTC CTATCCGGAG CGTCGCCTGC GGGGTGTCCT GGAGGAGCTC 
AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCGTCCCGC 
ACCAGGCTGG ACAGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAGAG TATGGGGCAG 
CAGGCCAAGA CCTTGCGGGC ACAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAATT TCCTGCAGAA ACTGCGCTTC CTGGCGGACG AGCCTCAGCA CAGCATCCCC 
GACGTCTTCA TCTGGATGAT GAACAACAAC AAGCGCACCG CCTACGCCAG GGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TCAAGCTGCC GGGCAAGCGG GGCTTCGGCT CCGTGGGCTG GACGGTGCAG 
GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTGAGCAAGC AGCGCAAGGA CTTCCTGTGC 
GGCCTGCCCT GCGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACGCCTTC 
CCGCCCATCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGGGC CCACATGTAC 
CAGGCTCGCA GCCTCTTCGC TGCCGACAGC AGCGGCCTCT CGGACCCCTT CGCCCGCGTC 
TTCTTCATCA ACCAGAGTCA GTGCACGGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTATATGGCG AAGCCCATGA GTTGCGGGAT 
GACCCCCCCA TCATCGTCAT CGAAATCTAC GACCAGGACA CCATGGGCAA AGCCGACTTC 
ATGGGCCGGA CCTTCGCCAA GCCCCTGGTG AAGATGGCGG ACGAGGCGTA CTGTCCACCC 
CGCTTCCCAC CCCAGCTCGA GTACTACCAG ATCTACCGCG GCAACGCCAC TGCCGGGGAC 
CTGCTGGCTG CCTTCGAGTT GCTGCAGATT GGGCCTGCGG GGAAGGCAGA CCTGCCACCC 
ATCAACGGCC CGGGGGACAT GGACCGAGGT CCCATCATGC CTGTGCCCGT CGGCATCCGG 
CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGCGGGA CCTGAAGCGG 
GTCAACCTGG CCCAGGTGGA CCGGCCGCGG GTAGACATCG AGTGTGCCGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCGGA GAACGAGCTG CTGCACCCGC CCCTGAACAT CCGAGTGGTG 
GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGTTCCC ACGCCGTCAG CTCTCTGAGG 
CGCTTCATCT ACAGGCCCCC AGACCGCTCG GCGCCCAACT GGAACACCAC GGGCAGGCTC 
CCTCGGGGTC GCCGTGTGCT GTGCGATGGC GGTCCCTCCT CTCACCCCCC AGGGGAGGTC 
GTGGTGAACA TGGAGCCAGA GGTGCCTGTC CGGAAGCTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG ATGCCGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAAGA GAAGAAGAAA 
AAGAAGAAAA AGGGCAGCTC AGAAGAGCCA GAGGAGGAGG AGCCCGATGA GAGCATGCTG 
GACTGGTGGT CCAAGTACTT TGCCTCCATC GATACCATGA AGGAGCAACT TCGACAGCAA 
GAGGCCTCCG GCATTGACTT GGAGGAGAAG GAGGAGGTGG AGAACACGGA GGGCCTAAAG 
GGGCCGATGA AGGGCAAGGA GAAGGCAAAG GCGGCGAAGG AGGAGAAGAA AAAGAGAACC 
CAGAGCCCCG GCACTGGCCA GGGGCCTGAG GCCCCAGAGA AGAAGAAACC CAAGATTGAT 
GAGCTTAAGG TGTACCCCAA GGAGCTGGAG TCTGAGTTTG ACAACTTTGA GGACTGGCTG 
CACACTTTCA ATCTGCTGCG GGGCAAGACG GGGGATGACG AGGACGGTTC CACGGAGGAG 
GAGCGCATCG TGGGCCGGTT CAAGGGCTCC CTCTGCGTGT ATAAAGTGCC ACTCCCAGAG 
GACGTGTCCC GGGAAGCCGG CTACGACCCC ACCTACGGCA TGTTCCAGGG CATCCCCAGC 
AACGACCCCA TCAATGTGCT GGTCCGAGTC TATGTGGTCA GGGCCACGGA CCTGCACCCC 
GCAGACATTA ACGGCAAAGC CGACCCCTAC ATTGCCATCC GGCTGGGCAA AACTGACATC 
CGAGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCTG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCCAT GGAATCCATG CTGACAGTGG CCGTGTACGA CTGGGATCTG 
GTAGGCACCG ATGACCTCAT CGGGGAAACC AAGATTGACC TGGAGAACCG CTTCTACAGC 
AAACACCGGG CCACCTGTGG CATCGCCCAG ACCTACTCCA TACATGGCTA CAATATCTGG 
CGGGACCCCA TGAAGCCCAG CCAGATTCTG ACCCGCCTCT GCAAGGAGGG CAAAGTGGAC 
GGCCCCCACT TTGGGCCCCC CGGGAGAGTG AAGGTGTCCA ACCGTGTCTT CACTGGGCCC 
TCCGAGATCG AGGACGAGAA TGGTCAGAGG AAGCCTACGG AAGAGCATGT GGCTCTATCT 
GCCCTGAGGC ACTGGGAGGA CATCCCCCGC GTGGGCTGCC GCCTGGTGCC CGAACACGTG 
GAAACCCGGC CCCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGCCG CCTGGAGCTG 
TGGGTGGACA TGTTCCCCAT GGACATGCCG GCCCCTGGGA CGCCTCTGGA CATCTCCCCC 
CGGAAGCCCA AGAAGTATGA GCTCCGGGTC ATCGTGTGGA ACACAGACGA GGTGGTCCTG 
GAGGACGATG ACTTCTTTAC TGGGGAGAAG TCCAGCGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA GCAGGACACG GACGTCCACT ATCATTCCCT CACCGGCGAG 
GGCAACTTCA ACTGGCGCTA CCTGTTCCCC TTCGACTACC TGGCCGCCGA GGAAAGGATT 
GTCATCTCCA AGAAGGAGTC CATGTTCTCC TGGGACGAGA CCGAGTACAA GATCCCCGCG 
CGGCTCACCC TGCAGATCTG GGACGCGGAC CACTTCTCCG CGGACGACTT CCTGGGGGCC 
ATCGAGCTGG ACCTCAACCG CTTTCCCCGG GGCGCCAAGA CAGCCAAGCA GTGCACCATG 
GAGATGGCCA CGGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTCAAGCA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTCGAGCT CACGGGCAAG 
GTGGAGGCAG AATTGCATCT ACTGACAGCG GAGGAGGCAG AGAAGAATCC GGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAGAAA CCCAACCGGC CTGACACCGC CTTCGTCTGG 
TTCCTCAACC CCCTCAAGTC CATCAAGTAC CTCATCTGCA CGCGGTACAA GTGGCTCATC 
ATCAAGATCG TGCTGGCGCT GCTGGGGCTG CTCATGCTGG CCCTCTTCCT CTACAGCCTC 
CCCGGCTACA TGGTCAAGAA GCTCCTGGGG GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683353.1
CDS	207..6002
Translation	MVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRSKGREKTKGGRDGEHKAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGRLPRGRRVLCDGGPSSHPPGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827794.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827794.1

ORF Insert Sequence:

ATGGTGCTGC AGAAGGTGGT GGAGGAGAAG CGTGTGGAGG TGACCGACAC ACTGATCGAT 
GACAACAACG CTATCATCAA GACCAGCCTG TGCGTGGAGG TCCGATATCA GGCCACAGAT 
GGCACGGTGG GCTCCTGGGA TGATGGAGAC TTCCTGGGGG ATGAGTCCCT TCATGAGGAA 
GAGAAGGACA GCCAGGAGAC GGATGGACTG CTCCCCGGCT CCCGCCCCAG CTCCCGGACA 
CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAGAGATGGC 
GAGCACAAAG CTGGGAGAAG TGTGTTCTCC GCCATGAAGC TCGGCAAGAA CCGGTCCCAC 
AAGGAGGAAC CCCAAAGACA AGATGAACCG GCCGTGCTGG AGATGGAGGA CCTTGACCGC 
TTGGCCGTTC GGCTGGGGGA TGGGCTGGAC CCTGACTCTG TGTCTCTGGC CTCTGTCACA 
GCTCTCACCA CCAATGTCTC CAACAAACGA TCCAAGCCAG ACATTAAGAT GGTGCCAGGT 
GCCGGGCGGC CCGTGGATTA CCAGGTCAGC ATCACCGTGA TTGAGGCTCG GCAGCTCGTG 
GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGCG ACGACAAGAA GTACACATCC 
ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TCGTCTTCGA CTTCCACGTC 
TCTCCGGACG TCATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG 
CTTCGAAGCG GTACCCTGGT GGGGTCCTTC AAGATGGATG TGGGAACTGT GTACTCCCAG 
CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCGG ACCCCGACGA CATCTCCGCC 
GGGCTGAAGG GCTACGTGAA GTGTGACGTG GCCGTGGTGG GCAAGGGGGA CAACATCAAG 
ACGCCCCACA AGGCCAACGA GACAGACGAG GATGACATTG AAGGGAACTT GCTGCTCCCC 
GAGGGGGTGC CCCCCGAACG CCAGTGGGCC CGGTTCTACG TGAAGATTTA CCGAGCAGAA 
GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCGTT CATCGGGGAG 
AACAAGGACC TGGTTGACCC CTACGTGCAA GTCTTCTTTG CCGGCCAGAA GGGCAAGACA 
TCAGTACAGA AAAGCAGCTA CGAGCCACTG TGGAATGAGC AGGTCGTCTT TACGGACCTG 
TTCCCCCCCC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ATGCCGACAA GGTCAACGAC 
GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CTAATGATGG AGACAAAGGC 
TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCTACACG CAACTACACG 
CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGGG AGGGCGTGTC GTTCCGCGCC 
CGCCTCATGC TGGGCCTGGC GGTGGAGATC CTGGACACCT CCAATCCTGA GCTGACCAGC 
TCCACGGAGG TGCAGGTGGA GCAGGCCACG CCTGTCTCGG AGAGCTGCAC GGGGAAAATG 
GAAGAATTCT TTCTATTCGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAATGGC 
GACAAGCCAA TCACCTTTGA GGTGACCATA GGCAACTATG GGAATGAAGT TGATGGCCTG 
TCGCGGCCCC AGCGGCCTAG GCCCCGGAAG GAGCCTGGGG ATGAGGAAGA AGTAGATCTG 
ATCCAGAACT CCAGTGATGA AGATGCCGGT GAGGGTGGGG ACCTGGCCTC GGTGTCCTCG 
ACTCCGCCCA TGCGGCCCCA GATCACCGAC AGGAACTACT TCCATCTGCC CTACCTGGAG 
CGCAAGCCCT GCATCTACAT CAAGAGCTGG TGGCCCGACC AGCGTCGCCG CCTCTACAAC 
GCCAACATCA TGGACCACAT CGCGGACAAG CTGGAAGAAG GCCTGAACGA CGTGCAGGAG 
ATGATAAAGA CAGAGAAGTC CTATCCGGAG CGTCGCCTGC GGGGTGTCCT GGAGGAGCTC 
AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCGTCCCGC 
ACCAGGCTGG ACAGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAGAG TATGGGGCAG 
CAGGCCAAGA CCTTGCGGGC ACAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAATT TCCTGCAGAA ACTGCGCTTC CTGGCGGACG AGCCTCAGCA CAGCATCCCC 
GACGTCTTCA TCTGGATGAT GAACAACAAC AAGCGCACCG CCTACGCCAG GGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TCAAGCTGCC GGGCAAGCGG GGCTTCGGCT CCGTGGGCTG GACGGTGCAG 
GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTGAGCAAGC AGCGCAAGGA CTTCCTGTGC 
GGCCTGCCCT GCGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACGCCTTC 
CCGCCCATCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGGGC CCACATGTAC 
CAGGCTCGCA GCCTCTTCGC TGCCGACAGC AGCGGCCTCT CGGACCCCTT CGCCCGCGTC 
TTCTTCATCA ACCAGAGTCA GTGCACGGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTATATGGCG AAGCCCATGA GTTGCGGGAT 
GACCCCCCCA TCATCGTCAT CGAAATCTAC GACCAGGACA CCATGGGCAA AGCCGACTTC 
ATGGGCCGGA CCTTCGCCAA GCCCCTGGTG AAGATGGCGG ACGAGGCGTA CTGTCCACCC 
CGCTTCCCAC CCCAGCTCGA GTACTACCAG ATCTACCGCG GCAACGCCAC TGCCGGGGAC 
CTGCTGGCTG CCTTCGAGTT GCTGCAGATT GGGCCTGCGG GGAAGGCAGA CCTGCCACCC 
ATCAACGGCC CGGGGGACAT GGACCGAGGT CCCATCATGC CTGTGCCCGT CGGCATCCGG 
CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGCGGGA CCTGAAGCGG 
GTCAACCTGG CCCAGGTGGA CCGGCCGCGG GTAGACATCG AGTGTGCCGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCGGA GAACGAGCTG CTGCACCCGC CCCTGAACAT CCGAGTGGTG 
GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGTTCCC ACGCCGTCAG CTCTCTGAGG 
CGCTTCATCT ACAGGCCCCC AGACCGCTCG GCGCCCAACT GGAACACCAC GGGCAGGCTC 
CCTCGGGGTC GCCGTGTGCT GTGCGATGGC GGTCCCTCCT CTCACCCCCC AGGGGAGGTC 
GTGGTGAACA TGGAGCCAGA GGTGCCTGTC CGGAAGCTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG ATGCCGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAAGA GAAGAAGAAA 
AAGAAGAAAA AGGGCAGCTC AGAAGAGCCA GAGGAGGAGG AGCCCGATGA GAGCATGCTG 
GACTGGTGGT CCAAGTACTT TGCCTCCATC GATACCATGA AGGAGCAACT TCGACAGCAA 
GAGGCCTCCG GCATTGACTT GGAGGAGAAG GAGGAGGTGG AGAACACGGA GGGCCTAAAG 
GGGCCGATGA AGGGCAAGGA GAAGGCAAAG GCGGCGAAGG AGGAGAAGAA AAAGAGAACC 
CAGAGCCCCG GCACTGGCCA GGGGCCTGAG GCCCCAGAGA AGAAGAAACC CAAGATTGAT 
GAGCTTAAGG TGTACCCCAA GGAGCTGGAG TCTGAGTTTG ACAACTTTGA GGACTGGCTG 
CACACTTTCA ATCTGCTGCG GGGCAAGACG GGGGATGACG AGGACGGTTC CACGGAGGAG 
GAGCGCATCG TGGGCCGGTT CAAGGGCTCC CTCTGCGTGT ATAAAGTGCC ACTCCCAGAG 
GACGTGTCCC GGGAAGCCGG CTACGACCCC ACCTACGGCA TGTTCCAGGG CATCCCCAGC 
AACGACCCCA TCAATGTGCT GGTCCGAGTC TATGTGGTCA GGGCCACGGA CCTGCACCCC 
GCAGACATTA ACGGCAAAGC CGACCCCTAC ATTGCCATCC GGCTGGGCAA AACTGACATC 
CGAGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCTG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCCAT GGAATCCATG CTGACAGTGG CCGTGTACGA CTGGGATCTG 
GTAGGCACCG ATGACCTCAT CGGGGAAACC AAGATTGACC TGGAGAACCG CTTCTACAGC 
AAACACCGGG CCACCTGTGG CATCGCCCAG ACCTACTCCA TACATGGCTA CAATATCTGG 
CGGGACCCCA TGAAGCCCAG CCAGATTCTG ACCCGCCTCT GCAAGGAGGG CAAAGTGGAC 
GGCCCCCACT TTGGGCCCCC CGGGAGAGTG AAGGTGTCCA ACCGTGTCTT CACTGGGCCC 
TCCGAGATCG AGGACGAGAA TGGTCAGAGG AAGCCTACGG AAGAGCATGT GGCTCTATCT 
GCCCTGAGGC ACTGGGAGGA CATCCCCCGC GTGGGCTGCC GCCTGGTGCC CGAACACGTG 
GAAACCCGGC CCCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGCCG CCTGGAGCTG 
TGGGTGGACA TGTTCCCCAT GGACATGCCG GCCCCTGGGA CGCCTCTGGA CATCTCCCCC 
CGGAAGCCCA AGAAGTATGA GCTCCGGGTC ATCGTGTGGA ACACAGACGA GGTGGTCCTG 
GAGGACGATG ACTTCTTTAC TGGGGAGAAG TCCAGCGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA GCAGGACACG GACGTCCACT ATCATTCCCT CACCGGCGAG 
GGCAACTTCA ACTGGCGCTA CCTGTTCCCC TTCGACTACC TGGCCGCCGA GGAAAGGATT 
GTCATCTCCA AGAAGGAGTC CATGTTCTCC TGGGACGAGA CCGAGTACAA GATCCCCGCG 
CGGCTCACCC TGCAGATCTG GGACGCGGAC CACTTCTCCG CGGACGACTT CCTGGGGGCC 
ATCGAGCTGG ACCTCAACCG CTTTCCCCGG GGCGCCAAGA CAGCCAAGCA GTGCACCATG 
GAGATGGCCA CGGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTCAAGCA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTCGAGCT CACGGGCAAG 
GTGGAGGCAG AATTGCATCT ACTGACAGCG GAGGAGGCAG AGAAGAATCC GGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAGAAA CCCAACCGGC CTGACACCGC CTTCGTCTGG 
TTCCTCAACC CCCTCAAGTC CATCAAGTAC CTCATCTGCA CGCGGTACAA GTGGCTCATC 
ATCAAGATCG TGCTGGCGCT GCTGGGGCTG CTCATGCTGG CCCTCTTCCT CTACAGCCTC 
CCCGGCTACA TGGTCAAGAA GCTCCTGGGG GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_019827795.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683354.1 Felis catus otoferlin (OTOF), transcript variant X6, mRNA.

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CloneID	OFb40729
Clone ID Related Accession (Same CDS sequence)	XM_019827795.1
Accession Version	XM_019827795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	otoferlin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG GCAAGAACCG GTCCCACAAG GAGGAACCCC AAAGACAAGA TGAACCGGCC 
GTGCTGGAGA TGGAGGACCT TGACCGCTTG GCCGTTCGGC TGGGGGATGG GCTGGACCCT 
GACTCTGTGT CTCTGGCCTC TGTCACAGCT CTCACCACCA ATGTCTCCAA CAAACGATCC 
AAGCCAGACA TTAAGATGGT GCCAGGTGCC GGGCGGCCCG TGGATTACCA GGTCAGCATC 
ACCGTGATTG AGGCTCGGCA GCTCGTGGGC TTGAACATGG ACCCCGTGGT GTGCGTGGAG 
GTGGGCGACG ACAAGAAGTA CACATCCATG AAGGAGTCCA CTAACTGCCC CTACTACAAT 
GAGTACTTCG TCTTCGACTT CCACGTCTCT CCGGACGTCA TGTTCGACAA GATCATCAAG 
ATCTCGGTGA TTCACTCCAA GAACCTGCTT CGAAGCGGTA CCCTGGTGGG GTCCTTCAAG 
ATGGATGTGG GAACTGTGTA CTCCCAGCCT GAGCACCAGT TCCACCACAA GTGGGCCATC 
CTGTCGGACC CCGACGACAT CTCCGCCGGG CTGAAGGGCT ACGTGAAGTG TGACGTGGCC 
GTGGTGGGCA AGGGGGACAA CATCAAGACG CCCCACAAGG CCAACGAGAC AGACGAGGAT 
GACATTGAAG GGAACTTGCT GCTCCCCGAG GGGGTGCCCC CCGAACGCCA GTGGGCCCGG 
TTCTACGTGA AGATTTACCG AGCAGAAGGG CTGCCCCGTA TGAACACCAG CCTCATGGCC 
AATGTGAAGA AGGCGTTCAT CGGGGAGAAC AAGGACCTGG TTGACCCCTA CGTGCAAGTC 
TTCTTTGCCG GCCAGAAGGG CAAGACATCA GTACAGAAAA GCAGCTACGA GCCACTGTGG 
AATGAGCAGG TCGTCTTTAC GGACCTGTTC CCCCCCCTCT GCAAGCGCAT GAAGGTGCAG 
ATCCGAGATG CCGACAAGGT CAACGACGTG GCCATCGGCA CCCACTTCAT TGACCTGCGC 
AAGATTTCTA ATGATGGAGA CAAAGGCTTC CTGCCCACGC TGGGCCCGGC CTGGGTGAAC 
ATGTACGGCT CTACACGCAA CTACACGCTG CTGGACGAGC ACCAGGACCT GAATGAGGGC 
CTGGGGGAGG GCGTGTCGTT CCGCGCCCGC CTCATGCTGG GCCTGGCGGT GGAGATCCTG 
GACACCTCCA ATCCTGAGCT GACCAGCTCC ACGGAGGTGC AGGTGGAGCA GGCCACGCCT 
GTCTCGGAGA GCTGCACGGG GAAAATGGAA GAATTCTTTC TATTCGGAGC CTTCCTAGAG 
GCCTCAATGA TCGACCGGAA AAATGGCGAC AAGCCAATCA CCTTTGAGGT GACCATAGGC 
AACTATGGGA ATGAAGTTGA TGGCCTGTCG CGGCCCCAGC GGCCTAGGCC CCGGAAGGAG 
CCTGGGGATG AGGAAGAAGT AGATCTGATC CAGAACTCCA GTGATGAAGA TGCCGGTGAG 
GGTGGGGACC TGGCCTCGGT GTCCTCGACT CCGCCCATGC GGCCCCAGAT CACCGACAGG 
AACTACTTCC ATCTGCCCTA CCTGGAGCGC AAGCCCTGCA TCTACATCAA GAGCTGGTGG 
CCCGACCAGC GTCGCCGCCT CTACAACGCC AACATCATGG ACCACATCGC GGACAAGCTG 
GAAGAAGGCC TGAACGACGT GCAGGAGATG ATAAAGACAG AGAAGTCCTA TCCGGAGCGT 
CGCCTGCGGG GTGTCCTGGA GGAGCTCAGC TGTGGCTGCT ACCGCTTCCT CTCCCTCGCT 
GACAAGGACC AGGGCCACTC GTCCCGCACC AGGCTGGACA GGGAGCGCCT CAAGTCCTGC 
ATGCGGGAGC TGGAGAGTAT GGGGCAGCAG GCCAAGACCT TGCGGGCACA GGTGAAGCGG 
CACACGGTGC GGGACAAGCT GAGGCTGTGC CAGAATTTCC TGCAGAAACT GCGCTTCCTG 
GCGGACGAGC CTCAGCACAG CATCCCCGAC GTCTTCATCT GGATGATGAA CAACAACAAG 
CGCACCGCCT ACGCCAGGGT GCCCTCCAAG GACCTGCTCT TCTCCATCGT TGAGGAGGAG 
CTGGGCAAGG ACTGCGCCAA GGTCAAGACG CTCTTCCTCA AGCTGCCGGG CAAGCGGGGC 
TTCGGCTCCG TGGGCTGGAC GGTGCAGGCC AAGCTGGAGC TCTACCTGTG GCTGGGCCTG 
AGCAAGCAGC GCAAGGACTT CCTGTGCGGC CTGCCCTGCG GCTTCGAGGA GGTCAAGGCA 
GCCCAGGGCC TGGGCCTGCA CGCCTTCCCG CCCATCAGCC TGGTCTACAC CAAGAAGCAG 
GCGTTCCAGC TCCGGGCCCA CATGTACCAG GCTCGCAGCC TCTTCGCTGC CGACAGCAGC 
GGCCTCTCGG ACCCCTTCGC CCGCGTCTTC TTCATCAACC AGAGTCAGTG CACGGAGGTG 
CTGAATGAGA CCCTGTGCCC CACCTGGGAC CAGATGCTGG TGTTCGACAA CCTGGAGCTA 
TATGGCGAAG CCCATGAGTT GCGGGATGAC CCCCCCATCA TCGTCATCGA AATCTACGAC 
CAGGACACCA TGGGCAAAGC CGACTTCATG GGCCGGACCT TCGCCAAGCC CCTGGTGAAG 
ATGGCGGACG AGGCGTACTG TCCACCCCGC TTCCCACCCC AGCTCGAGTA CTACCAGATC 
TACCGCGGCA ACGCCACTGC CGGGGACCTG CTGGCTGCCT TCGAGTTGCT GCAGATTGGG 
CCTGCGGGGA AGGCAGACCT GCCACCCATC AACGGCCCGG GGGACATGGA CCGAGGTCCC 
ATCATGCCTG TGCCCGTCGG CATCCGGCCC GTGCTCAGCA AATACCGAAT CGAGGTGCTC 
TTCTGGGGCC TGCGGGACCT GAAGCGGGTC AACCTGGCCC AGGTGGACCG GCCGCGGGTA 
GACATCGAGT GTGCCGGGAA GGGGGTGCAG TCGTCCCTGA TCCACAATTA CAAGAAGAAC 
CCCAACTTCA ACACCCTCGT CAAGTGGTTT GAAGTGGACC TCCCGGAGAA CGAGCTGCTG 
CACCCGCCCC TGAACATCCG AGTGGTGGAC TGCCGGGCCT TCGGCCGCTA CACCCTGGTG 
GGTTCCCACG CCGTCAGCTC TCTGAGGCGC TTCATCTACA GGCCCCCAGA CCGCTCGGCG 
CCCAACTGGA ACACCACGGG CAGGCTCCCT CGGGGTCGCC GTGTGCTGTG CGATGGCGGT 
CCCTCCTCTC ACCCCCCAGG GGAGGTCGTG GTGAACATGG AGCCAGAGGT GCCTGTCCGG 
AAGCTGGAGA CCATGGTGAA GCTGGACGCG ACTTCTGATG CCGTCATCAA GGTGGATGTG 
GCTGACGAGG AGAAAGAGAA GAAGAAAAAG AAGAAAAAGG GCAGCTCAGA AGAGCCAGAG 
GAGGAGGAGC CCGATGAGAG CATGCTGGAC TGGTGGTCCA AGTACTTTGC CTCCATCGAT 
ACCATGAAGG AGCAACTTCG ACAGCAAGAG GCCTCCGGCA TTGACTTGGA GGAGAAGGAG 
GAGGTGGAGA ACACGGAGGG CCTAAAGGGG CCGATGAAGG GCAAGGAGAA GGCAAAGGCG 
GCGAAGGAGG AGAAGAAAAA GAGAACCCAG AGCCCCGGCA CTGGCCAGGG GCCTGAGGCC 
CCAGAGAAGA AGAAACCCAA GATTGATGAG CTTAAGGTGT ACCCCAAGGA GCTGGAGTCT 
GAGTTTGACA ACTTTGAGGA CTGGCTGCAC ACTTTCAATC TGCTGCGGGG CAAGACGGGG 
GATGACGAGG ACGGTTCCAC GGAGGAGGAG CGCATCGTGG GCCGGTTCAA GGGCTCCCTC 
TGCGTGTATA AAGTGCCACT CCCAGAGGAC GTGTCCCGGG AAGCCGGCTA CGACCCCACC 
TACGGCATGT TCCAGGGCAT CCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
GTGGTCAGGG CCACGGACCT GCACCCCGCA GACATTAACG GCAAAGCCGA CCCCTACATT 
GCCATCCGGC TGGGCAAAAC TGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC 
AACCCTGTCT TTGGGAAGTC CTTTGACATC GAGGCCTCCT TCCCCATGGA ATCCATGCTG 
ACAGTGGCCG TGTACGACTG GGATCTGGTA GGCACCGATG ACCTCATCGG GGAAACCAAG 
ATTGACCTGG AGAACCGCTT CTACAGCAAA CACCGGGCCA CCTGTGGCAT CGCCCAGACC 
TACTCCATAC ATGGCTACAA TATCTGGCGG GACCCCATGA AGCCCAGCCA GATTCTGACC 
CGCCTCTGCA AGGAGGGCAA AGTGGACGGC CCCCACTTTG GGCCCCCCGG GAGAGTGAAG 
GTGTCCAACC GTGTCTTCAC TGGGCCCTCC GAGATCGAGG ACGAGAATGG TCAGAGGAAG 
CCTACGGAAG AGCATGTGGC TCTATCTGCC CTGAGGCACT GGGAGGACAT CCCCCGCGTG 
GGCTGCCGCC TGGTGCCCGA ACACGTGGAA ACCCGGCCCC TGCTCAACCC TGACAAGCCA 
GGCATCGAGC AGGGCCGCCT GGAGCTGTGG GTGGACATGT TCCCCATGGA CATGCCGGCC 
CCTGGGACGC CTCTGGACAT CTCCCCCCGG AAGCCCAAGA AGTATGAGCT CCGGGTCATC 
GTGTGGAACA CAGACGAGGT GGTCCTGGAG GACGATGACT TCTTTACTGG GGAGAAGTCC 
AGCGACATTT TTGTGCGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAGCA GGACACGGAC 
GTCCACTATC ATTCCCTCAC CGGCGAGGGC AACTTCAACT GGCGCTACCT GTTCCCCTTC 
GACTACCTGG CCGCCGAGGA AAGGATTGTC ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG 
GACGAGACCG AGTACAAGAT CCCCGCGCGG CTCACCCTGC AGATCTGGGA CGCGGACCAC 
TTCTCCGCGG ACGACTTCCT GGGGGCCATC GAGCTGGACC TCAACCGCTT TCCCCGGGGC 
GCCAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACGG GGGAGGTGGA CGTGCCCCTG 
GTTTCCATCT TCAAGCAGAA GCGCGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG 
AATGATGAGT TCGAGCTCAC GGGCAAGGTG GAGGCAGAAT TGCATCTACT GACAGCGGAG 
GAGGCAGAGA AGAATCCGGT GGGCCTGGCC CGCAATGAAC CTGACCCTCT AGAGAAACCC 
AACCGGCCTG ACACCGCCTT CGTCTGGTTC CTCAACCCCC TCAAGTCCAT CAAGTACCTC 
ATCTGCACGC GGTACAAGTG GCTCATCATC AAGATCGTGC TGGCGCTGCT GGGGCTGCTC 
ATGCTGGCCC TCTTCCTCTA CAGCCTCCCC GGCTACATGG TCAAGAAGCT CCTGGGGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683354.1
CDS	261..5723
Translation	MKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGRLPRGRRVLCDGGPSSHPPGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827795.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827795.1

ORF Insert Sequence:

ATGAAGCTCG GCAAGAACCG GTCCCACAAG GAGGAACCCC AAAGACAAGA TGAACCGGCC 
GTGCTGGAGA TGGAGGACCT TGACCGCTTG GCCGTTCGGC TGGGGGATGG GCTGGACCCT 
GACTCTGTGT CTCTGGCCTC TGTCACAGCT CTCACCACCA ATGTCTCCAA CAAACGATCC 
AAGCCAGACA TTAAGATGGT GCCAGGTGCC GGGCGGCCCG TGGATTACCA GGTCAGCATC 
ACCGTGATTG AGGCTCGGCA GCTCGTGGGC TTGAACATGG ACCCCGTGGT GTGCGTGGAG 
GTGGGCGACG ACAAGAAGTA CACATCCATG AAGGAGTCCA CTAACTGCCC CTACTACAAT 
GAGTACTTCG TCTTCGACTT CCACGTCTCT CCGGACGTCA TGTTCGACAA GATCATCAAG 
ATCTCGGTGA TTCACTCCAA GAACCTGCTT CGAAGCGGTA CCCTGGTGGG GTCCTTCAAG 
ATGGATGTGG GAACTGTGTA CTCCCAGCCT GAGCACCAGT TCCACCACAA GTGGGCCATC 
CTGTCGGACC CCGACGACAT CTCCGCCGGG CTGAAGGGCT ACGTGAAGTG TGACGTGGCC 
GTGGTGGGCA AGGGGGACAA CATCAAGACG CCCCACAAGG CCAACGAGAC AGACGAGGAT 
GACATTGAAG GGAACTTGCT GCTCCCCGAG GGGGTGCCCC CCGAACGCCA GTGGGCCCGG 
TTCTACGTGA AGATTTACCG AGCAGAAGGG CTGCCCCGTA TGAACACCAG CCTCATGGCC 
AATGTGAAGA AGGCGTTCAT CGGGGAGAAC AAGGACCTGG TTGACCCCTA CGTGCAAGTC 
TTCTTTGCCG GCCAGAAGGG CAAGACATCA GTACAGAAAA GCAGCTACGA GCCACTGTGG 
AATGAGCAGG TCGTCTTTAC GGACCTGTTC CCCCCCCTCT GCAAGCGCAT GAAGGTGCAG 
ATCCGAGATG CCGACAAGGT CAACGACGTG GCCATCGGCA CCCACTTCAT TGACCTGCGC 
AAGATTTCTA ATGATGGAGA CAAAGGCTTC CTGCCCACGC TGGGCCCGGC CTGGGTGAAC 
ATGTACGGCT CTACACGCAA CTACACGCTG CTGGACGAGC ACCAGGACCT GAATGAGGGC 
CTGGGGGAGG GCGTGTCGTT CCGCGCCCGC CTCATGCTGG GCCTGGCGGT GGAGATCCTG 
GACACCTCCA ATCCTGAGCT GACCAGCTCC ACGGAGGTGC AGGTGGAGCA GGCCACGCCT 
GTCTCGGAGA GCTGCACGGG GAAAATGGAA GAATTCTTTC TATTCGGAGC CTTCCTAGAG 
GCCTCAATGA TCGACCGGAA AAATGGCGAC AAGCCAATCA CCTTTGAGGT GACCATAGGC 
AACTATGGGA ATGAAGTTGA TGGCCTGTCG CGGCCCCAGC GGCCTAGGCC CCGGAAGGAG 
CCTGGGGATG AGGAAGAAGT AGATCTGATC CAGAACTCCA GTGATGAAGA TGCCGGTGAG 
GGTGGGGACC TGGCCTCGGT GTCCTCGACT CCGCCCATGC GGCCCCAGAT CACCGACAGG 
AACTACTTCC ATCTGCCCTA CCTGGAGCGC AAGCCCTGCA TCTACATCAA GAGCTGGTGG 
CCCGACCAGC GTCGCCGCCT CTACAACGCC AACATCATGG ACCACATCGC GGACAAGCTG 
GAAGAAGGCC TGAACGACGT GCAGGAGATG ATAAAGACAG AGAAGTCCTA TCCGGAGCGT 
CGCCTGCGGG GTGTCCTGGA GGAGCTCAGC TGTGGCTGCT ACCGCTTCCT CTCCCTCGCT 
GACAAGGACC AGGGCCACTC GTCCCGCACC AGGCTGGACA GGGAGCGCCT CAAGTCCTGC 
ATGCGGGAGC TGGAGAGTAT GGGGCAGCAG GCCAAGACCT TGCGGGCACA GGTGAAGCGG 
CACACGGTGC GGGACAAGCT GAGGCTGTGC CAGAATTTCC TGCAGAAACT GCGCTTCCTG 
GCGGACGAGC CTCAGCACAG CATCCCCGAC GTCTTCATCT GGATGATGAA CAACAACAAG 
CGCACCGCCT ACGCCAGGGT GCCCTCCAAG GACCTGCTCT TCTCCATCGT TGAGGAGGAG 
CTGGGCAAGG ACTGCGCCAA GGTCAAGACG CTCTTCCTCA AGCTGCCGGG CAAGCGGGGC 
TTCGGCTCCG TGGGCTGGAC GGTGCAGGCC AAGCTGGAGC TCTACCTGTG GCTGGGCCTG 
AGCAAGCAGC GCAAGGACTT CCTGTGCGGC CTGCCCTGCG GCTTCGAGGA GGTCAAGGCA 
GCCCAGGGCC TGGGCCTGCA CGCCTTCCCG CCCATCAGCC TGGTCTACAC CAAGAAGCAG 
GCGTTCCAGC TCCGGGCCCA CATGTACCAG GCTCGCAGCC TCTTCGCTGC CGACAGCAGC 
GGCCTCTCGG ACCCCTTCGC CCGCGTCTTC TTCATCAACC AGAGTCAGTG CACGGAGGTG 
CTGAATGAGA CCCTGTGCCC CACCTGGGAC CAGATGCTGG TGTTCGACAA CCTGGAGCTA 
TATGGCGAAG CCCATGAGTT GCGGGATGAC CCCCCCATCA TCGTCATCGA AATCTACGAC 
CAGGACACCA TGGGCAAAGC CGACTTCATG GGCCGGACCT TCGCCAAGCC CCTGGTGAAG 
ATGGCGGACG AGGCGTACTG TCCACCCCGC TTCCCACCCC AGCTCGAGTA CTACCAGATC 
TACCGCGGCA ACGCCACTGC CGGGGACCTG CTGGCTGCCT TCGAGTTGCT GCAGATTGGG 
CCTGCGGGGA AGGCAGACCT GCCACCCATC AACGGCCCGG GGGACATGGA CCGAGGTCCC 
ATCATGCCTG TGCCCGTCGG CATCCGGCCC GTGCTCAGCA AATACCGAAT CGAGGTGCTC 
TTCTGGGGCC TGCGGGACCT GAAGCGGGTC AACCTGGCCC AGGTGGACCG GCCGCGGGTA 
GACATCGAGT GTGCCGGGAA GGGGGTGCAG TCGTCCCTGA TCCACAATTA CAAGAAGAAC 
CCCAACTTCA ACACCCTCGT CAAGTGGTTT GAAGTGGACC TCCCGGAGAA CGAGCTGCTG 
CACCCGCCCC TGAACATCCG AGTGGTGGAC TGCCGGGCCT TCGGCCGCTA CACCCTGGTG 
GGTTCCCACG CCGTCAGCTC TCTGAGGCGC TTCATCTACA GGCCCCCAGA CCGCTCGGCG 
CCCAACTGGA ACACCACGGG CAGGCTCCCT CGGGGTCGCC GTGTGCTGTG CGATGGCGGT 
CCCTCCTCTC ACCCCCCAGG GGAGGTCGTG GTGAACATGG AGCCAGAGGT GCCTGTCCGG 
AAGCTGGAGA CCATGGTGAA GCTGGACGCG ACTTCTGATG CCGTCATCAA GGTGGATGTG 
GCTGACGAGG AGAAAGAGAA GAAGAAAAAG AAGAAAAAGG GCAGCTCAGA AGAGCCAGAG 
GAGGAGGAGC CCGATGAGAG CATGCTGGAC TGGTGGTCCA AGTACTTTGC CTCCATCGAT 
ACCATGAAGG AGCAACTTCG ACAGCAAGAG GCCTCCGGCA TTGACTTGGA GGAGAAGGAG 
GAGGTGGAGA ACACGGAGGG CCTAAAGGGG CCGATGAAGG GCAAGGAGAA GGCAAAGGCG 
GCGAAGGAGG AGAAGAAAAA GAGAACCCAG AGCCCCGGCA CTGGCCAGGG GCCTGAGGCC 
CCAGAGAAGA AGAAACCCAA GATTGATGAG CTTAAGGTGT ACCCCAAGGA GCTGGAGTCT 
GAGTTTGACA ACTTTGAGGA CTGGCTGCAC ACTTTCAATC TGCTGCGGGG CAAGACGGGG 
GATGACGAGG ACGGTTCCAC GGAGGAGGAG CGCATCGTGG GCCGGTTCAA GGGCTCCCTC 
TGCGTGTATA AAGTGCCACT CCCAGAGGAC GTGTCCCGGG AAGCCGGCTA CGACCCCACC 
TACGGCATGT TCCAGGGCAT CCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
GTGGTCAGGG CCACGGACCT GCACCCCGCA GACATTAACG GCAAAGCCGA CCCCTACATT 
GCCATCCGGC TGGGCAAAAC TGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC 
AACCCTGTCT TTGGGAAGTC CTTTGACATC GAGGCCTCCT TCCCCATGGA ATCCATGCTG 
ACAGTGGCCG TGTACGACTG GGATCTGGTA GGCACCGATG ACCTCATCGG GGAAACCAAG 
ATTGACCTGG AGAACCGCTT CTACAGCAAA CACCGGGCCA CCTGTGGCAT CGCCCAGACC 
TACTCCATAC ATGGCTACAA TATCTGGCGG GACCCCATGA AGCCCAGCCA GATTCTGACC 
CGCCTCTGCA AGGAGGGCAA AGTGGACGGC CCCCACTTTG GGCCCCCCGG GAGAGTGAAG 
GTGTCCAACC GTGTCTTCAC TGGGCCCTCC GAGATCGAGG ACGAGAATGG TCAGAGGAAG 
CCTACGGAAG AGCATGTGGC TCTATCTGCC CTGAGGCACT GGGAGGACAT CCCCCGCGTG 
GGCTGCCGCC TGGTGCCCGA ACACGTGGAA ACCCGGCCCC TGCTCAACCC TGACAAGCCA 
GGCATCGAGC AGGGCCGCCT GGAGCTGTGG GTGGACATGT TCCCCATGGA CATGCCGGCC 
CCTGGGACGC CTCTGGACAT CTCCCCCCGG AAGCCCAAGA AGTATGAGCT CCGGGTCATC 
GTGTGGAACA CAGACGAGGT GGTCCTGGAG GACGATGACT TCTTTACTGG GGAGAAGTCC 
AGCGACATTT TTGTGCGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAGCA GGACACGGAC 
GTCCACTATC ATTCCCTCAC CGGCGAGGGC AACTTCAACT GGCGCTACCT GTTCCCCTTC 
GACTACCTGG CCGCCGAGGA AAGGATTGTC ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG 
GACGAGACCG AGTACAAGAT CCCCGCGCGG CTCACCCTGC AGATCTGGGA CGCGGACCAC 
TTCTCCGCGG ACGACTTCCT GGGGGCCATC GAGCTGGACC TCAACCGCTT TCCCCGGGGC 
GCCAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACGG GGGAGGTGGA CGTGCCCCTG 
GTTTCCATCT TCAAGCAGAA GCGCGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG 
AATGATGAGT TCGAGCTCAC GGGCAAGGTG GAGGCAGAAT TGCATCTACT GACAGCGGAG 
GAGGCAGAGA AGAATCCGGT GGGCCTGGCC CGCAATGAAC CTGACCCTCT AGAGAAACCC 
AACCGGCCTG ACACCGCCTT CGTCTGGTTC CTCAACCCCC TCAAGTCCAT CAAGTACCTC 
ATCTGCACGC GGTACAAGTG GCTCATCATC AAGATCGTGC TGGCGCTGCT GGGGCTGCTC 
ATGCTGGCCC TCTTCCTCTA CAGCCTCCCC GGCTACATGG TCAAGAAGCT CCTGGGGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_019827790.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683349.1 Felis catus otoferlin (OTOF), transcript variant X1, mRNA.

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CloneID	OFb73951
Clone ID Related Accession (Same CDS sequence)	XM_019827790.2
Accession Version	XM_019827790.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019827790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGCAGGCT CCCTCGGGGT CGCCGTGTGC TGTGCGATGG CGGTCCCTCC 
TCTCACCCCC CAGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCAC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683349.1
CDS	1..5997
Translation	MALFVHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENREDVADFDETFRWPVASSIDSNEILEIQIFNYSKVFSNKLIGTFRMVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGRLPRGRRVLCDGGPSSHPPGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827790.2
Organism	Felis catus(domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827790.2

ORF Insert Sequence:

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGCAGGCT CCCTCGGGGT CGCCGTGTGC TGTGCGATGG CGGTCCCTCC 
TCTCACCCCC CAGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCAC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_019827793.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683352.1 Felis catus otoferlin (OTOF), transcript variant X2, mRNA.

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CloneID	OFb73952
Clone ID Related Accession (Same CDS sequence)	XM_019827793.2
Accession Version	XM_019827793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019827793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCAC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683352.1
CDS	1..5937
Translation	MALFVHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENREDVADFDETFRWPVASSIDSNEILEIQIFNYSKVFSNKLIGTFRMVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827793.2
Organism	Felis catus(domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827793.2

ORF Insert Sequence:

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAACCGG 
CCTGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGCTGGCAC TGCTGGGGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_023252023.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107791.1 Felis catus otoferlin (OTOF), transcript variant X3, mRNA.

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CloneID	OFb73953
Clone ID Related Accession (Same CDS sequence)	XM_023252023.1
Accession Version	XM_023252023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	otoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGC AGAAGGTGGT GGAGGAGAAG CGTGTGGAGG TGACCGACAC ACTGATCGAT 
GACAACAACG CTATCATCAA GACCAGCCTG TGCGTGGAGG TCCGATATCA GGCCACAGAT 
GGCACGGTGG GCTCCTGGGA TGATGGAGAC TTCCTGGGGG ATGAGTCCCT TCATGAGGAA 
GAGAAGGACA GCCAGGAGAC GGATGGACTG CTCCCCGGCT CCCGCCCCAG CTCCCGGACA 
CCAGGCGAGA AGAGCTTCCG GAGAGCTGGG AGAAGTGTGT TCTCCGCCAT GAAGCTCGGC 
AAGAACCGGT CCCACAAGGA GGAACCCCAA AGACAAGATG AACCGGCCGT GCTGGAGATG 
GAGGACCTTG ACCGCTTGGC CGTTCGGCTG GGGGATGGGC TGGACCCTGA CTCTGTGTCT 
CTGGCCTCTG TCACAGCTCT CACCACCAAT GTCTCCAACA AACGATCCAA GCCAGACATT 
AAGATGGTGC CAGGTGCCGG GCGGCCCGTG GATTACCAGG TCAGCATCAC CGTGATTGAG 
GCTCGGCAGC TCGTGGGCTT GAACATGGAC CCCGTGGTGT GCGTGGAGGT GGGCGACGAC 
AAGAAGTACA CATCCATGAA GGAGTCCACT AACTGCCCCT ACTACAATGA GTACTTCGTC 
TTCGACTTCC ACGTCTCTCC GGACGTCATG TTCGACAAGA TCATCAAGAT CTCGGTGATT 
CACTCCAAGA ACCTGCTTCG AAGCGGTACC CTGGTGGGGT CCTTCAAGAT GGATGTGGGA 
ACTGTGTACT CCCAGCCTGA GCACCAGTTC CACCACAAGT GGGCCATCCT GTCGGACCCC 
GACGACATCT CCGCCGGGCT GAAGGGCTAC GTGAAGTGTG ACGTGGCCGT GGTGGGCAAG 
GGGGACAACA TCAAGACGCC CCACAAGGCC AACGAGACAG ACGAGGATGA CATTGAAGGG 
AACTTGCTGC TCCCCGAGGG GGTGCCCCCC GAACGCCAGT GGGCCCGGTT CTACGTGAAG 
ATTTACCGAG CAGAAGGGCT GCCCCGTATG AACACCAGCC TCATGGCCAA TGTGAAGAAG 
GCGTTCATCG GGGAGAACAA GGACCTGGTT GACCCCTACG TGCAAGTCTT CTTTGCCGGC 
CAGAAGGGCA AGACATCAGT ACAGAAAAGC AGCTACGAGC CACTGTGGAA TGAGCAGGTC 
GTCTTTACGG ACCTGTTCCC CCCCCTCTGC AAGCGCATGA AGGTGCAGAT CCGAGATGCC 
GACAAGGTCA ACGACGTGGC CATCGGCACC CACTTCATTG ACCTGCGCAA GATTTCTAAT 
GATGGAGACA AAGGCTTCCT GCCCACGCTG GGCCCGGCCT GGGTGAACAT GTACGGCTCT 
ACACGCAACT ACACGCTGCT GGACGAGCAC CAGGACCTGA ATGAGGGCCT GGGGGAGGGC 
GTGTCGTTCC GCGCCCGCCT CATGCTGGGC CTGGCGGTGG AGATCCTGGA CACCTCCAAT 
CCTGAGCTGA CCAGCTCCAC GGAGGTGCAG GTGGAGCAGG CCACGCCTGT CTCGGAGAGC 
TGCACGGGGA AAATGGAAGA ATTCTTTCTA TTCGGAGCCT TCCTAGAGGC CTCAATGATC 
GACCGGAAAA ATGGCGACAA GCCAATCACC TTTGAGGTGA CCATAGGCAA CTATGGGAAT 
GAAGTTGATG GCCTGTCGCG GCCCCAGCGG CCTAGGCCCC GGAAGGAGCC TGGGGATGAG 
GAAGAAGTAG ATCTGATCCA GAACTCCAGT GATGAAGATG CCGGTGAGGG TGGGGACCTG 
GCCTCGGTGT CCTCGACTCC GCCCATGCGG CCCCAGATCA CCGACAGGAA CTACTTCCAT 
CTGCCCTACC TGGAGCGCAA GCCCTGCATC TACATCAAGA GCTGGTGGCC CGACCAGCGT 
CGCCGCCTCT ACAACGCCAA CATCATGGAC CACATCGCGG ACAAGCTGGA AGAAGGCCTG 
AACGACGTGC AGGAGATGAT AAAGACAGAG AAGTCCTATC CGGAGCGTCG CCTGCGGGGT 
GTCCTGGAGG AGCTCAGCTG TGGCTGCTAC CGCTTCCTCT CCCTCGCTGA CAAGGACCAG 
GGCCACTCGT CCCGCACCAG GCTGGACAGG GAGCGCCTCA AGTCCTGCAT GCGGGAGCTG 
GAGAGTATGG GGCAGCAGGC CAAGACCTTG CGGGCACAGG TGAAGCGGCA CACGGTGCGG 
GACAAGCTGA GGCTGTGCCA GAATTTCCTG CAGAAACTGC GCTTCCTGGC GGACGAGCCT 
CAGCACAGCA TCCCCGACGT CTTCATCTGG ATGATGAACA ACAACAAGCG CACCGCCTAC 
GCCAGGGTGC CCTCCAAGGA CCTGCTCTTC TCCATCGTTG AGGAGGAGCT GGGCAAGGAC 
TGCGCCAAGG TCAAGACGCT CTTCCTCAAG CTGCCGGGCA AGCGGGGCTT CGGCTCCGTG 
GGCTGGACGG TGCAGGCCAA GCTGGAGCTC TACCTGTGGC TGGGCCTGAG CAAGCAGCGC 
AAGGACTTCC TGTGCGGCCT GCCCTGCGGC TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG 
GGCCTGCACG CCTTCCCGCC CATCAGCCTG GTCTACACCA AGAAGCAGGC GTTCCAGCTC 
CGGGCCCACA TGTACCAGGC TCGCAGCCTC TTCGCTGCCG ACAGCAGCGG CCTCTCGGAC 
CCCTTCGCCC GCGTCTTCTT CATCAACCAG AGTCAGTGCA CGGAGGTGCT GAATGAGACC 
CTGTGCCCCA CCTGGGACCA GATGCTGGTG TTCGACAACC TGGAGCTATA TGGCGAAGCC 
CATGAGTTGC GGGATGACCC CCCCATCATC GTCATCGAAA TCTACGACCA GGACACCATG 
GGCAAAGCCG ACTTCATGGG CCGGACCTTC GCCAAGCCCC TGGTGAAGAT GGCGGACGAG 
GCGTACTGTC CACCCCGCTT CCCACCCCAG CTCGAGTACT ACCAGATCTA CCGCGGCAAC 
GCCACTGCCG GGGACCTGCT GGCTGCCTTC GAGTTGCTGC AGATTGGGCC TGCGGGGAAG 
GCAGACCTGC CACCCATCAA CGGCCCGGGG GACATGGACC GAGGTCCCAT CATGCCTGTG 
CCCGTCGGCA TCCGGCCCGT GCTCAGCAAA TACCGAATCG AGGTGCTCTT CTGGGGCCTG 
CGGGACCTGA AGCGGGTCAA CCTGGCCCAG GTGGACCGGC CGCGGGTAGA CATCGAGTGT 
GCCGGGAAGG GGGTGCAGTC GTCCCTGATC CACAATTACA AGAAGAACCC CAACTTCAAC 
ACCCTCGTCA AGTGGTTTGA AGTGGACCTC CCGGAGAACG AGCTGCTGCA CCCGCCCCTG 
AACATCCGAG TGGTGGACTG CCGGGCCTTC GGCCGCTACA CCCTGGTGGG TTCCCACGCC 
GTCAGCTCTC TGAGGCGCTT CATCTACAGG CCCCCAGACC GCTCGGCGCC CAACTGGAAC 
ACCACGGGCA GGCTCCCTCG GGGTCGCCGT GTGCTGTGCG ATGGCGGTCC CTCCTCTCAC 
CCCCCAGGGG AGGTCGTGGT GAACATGGAG CCAGAGGTGC CTGTCCGGAA GCTGGAGACC 
ATGGTGAAGC TGGACGCGAC TTCTGATGCC GTCATCAAGG TGGATGTGGC TGACGAGGAG 
AAAGAGAAGA AGAAAAAGAA GAAAAAGGGC AGCTCAGAAG AGCCAGAGGA GGAGGAGCCC 
GATGAGAGCA TGCTGGACTG GTGGTCCAAG TACTTTGCCT CCATCGATAC CATGAAGGAG 
CAACTTCGAC AGCAAGAGGC CTCCGGCATT GACTTGGAGG AGAAGGAGGA GGTGGAGAAC 
ACGGAGGGCC TAAAGGGGCC GATGAAGGGC AAGGAGAAGG CAAAGGCGGC GAAGGAGGAG 
AAGAAAAAGA GAACCCAGAG CCCCGGCACT GGCCAGGGGC CTGAGGCCCC AGAGAAGAAG 
AAACCCAAGA TTGATGAGCT TAAGGTGTAC CCCAAGGAGC TGGAGTCTGA GTTTGACAAC 
TTTGAGGACT GGCTGCACAC TTTCAATCTG CTGCGGGGCA AGACGGGGGA TGACGAGGAC 
GGTTCCACGG AGGAGGAGCG CATCGTGGGC CGGTTCAAGG GCTCCCTCTG CGTGTATAAA 
GTGCCACTCC CAGAGGACGT GTCCCGGGAA GCCGGCTACG ACCCCACCTA CGGCATGTTC 
CAGGGCATCC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATGT GGTCAGGGCC 
ACGGACCTGC ACCCCGCAGA CATTAACGGC AAAGCCGACC CCTACATTGC CATCCGGCTG 
GGCAAAACTG ACATCCGAGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCTGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCCATGGAAT CCATGCTGAC AGTGGCCGTG 
TACGACTGGG ATCTGGTAGG CACCGATGAC CTCATCGGGG AAACCAAGAT TGACCTGGAG 
AACCGCTTCT ACAGCAAACA CCGGGCCACC TGTGGCATCG CCCAGACCTA CTCCATACAT 
GGCTACAATA TCTGGCGGGA CCCCATGAAG CCCAGCCAGA TTCTGACCCG CCTCTGCAAG 
GAGGGCAAAG TGGACGGCCC CCACTTTGGG CCCCCCGGGA GAGTGAAGGT GTCCAACCGT 
GTCTTCACTG GGCCCTCCGA GATCGAGGAC GAGAATGGTC AGAGGAAGCC TACGGAAGAG 
CATGTGGCTC TATCTGCCCT GAGGCACTGG GAGGACATCC CCCGCGTGGG CTGCCGCCTG 
GTGCCCGAAC ACGTGGAAAC CCGGCCCCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGCCGCCTGG AGCTGTGGGT GGACATGTTC CCCATGGACA TGCCGGCCCC TGGGACGCCT 
CTGGACATCT CCCCCCGGAA GCCCAAGAAG TATGAGCTCC GGGTCATCGT GTGGAACACA 
GACGAGGTGG TCCTGGAGGA CGATGACTTC TTTACTGGGG AGAAGTCCAG CGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAGCAGG ACACGGACGT CCACTATCAT 
TCCCTCACCG GCGAGGGCAA CTTCAACTGG CGCTACCTGT TCCCCTTCGA CTACCTGGCC 
GCCGAGGAAA GGATTGTCAT CTCCAAGAAG GAGTCCATGT TCTCCTGGGA CGAGACCGAG 
TACAAGATCC CCGCGCGGCT CACCCTGCAG ATCTGGGACG CGGACCACTT CTCCGCGGAC 
GACTTCCTGG GGGCCATCGA GCTGGACCTC AACCGCTTTC CCCGGGGCGC CAAGACAGCC 
AAGCAGTGCA CCATGGAGAT GGCCACGGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTC 
AAGCAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTC 
GAGCTCACGG GCAAGGTGGA GGCAGAATTG CATCTACTGA CAGCGGAGGA GGCAGAGAAG 
AATCCGGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AGAAACCCAA CCGGCCTGAC 
ACCGCCTTCG TCTGGTTCCT CAACCCCCTC AAGTCCATCA AGTACCTCAT CTGCACGCGG 
TACAAGTGGC TCATCATCAA GATCGTGCTG GCACTGCTGG GGCTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCCGG CTACATGGTC AAGAAGCTCC TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107791.1
CDS	207..5957
Translation	MVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGRLPRGRRVLCDGGPSSHPPGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_023252023.1
Organism	Felis catus(domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252023.1

ORF Insert Sequence:

ATGGTGCTGC AGAAGGTGGT GGAGGAGAAG CGTGTGGAGG TGACCGACAC ACTGATCGAT 
GACAACAACG CTATCATCAA GACCAGCCTG TGCGTGGAGG TCCGATATCA GGCCACAGAT 
GGCACGGTGG GCTCCTGGGA TGATGGAGAC TTCCTGGGGG ATGAGTCCCT TCATGAGGAA 
GAGAAGGACA GCCAGGAGAC GGATGGACTG CTCCCCGGCT CCCGCCCCAG CTCCCGGACA 
CCAGGCGAGA AGAGCTTCCG GAGAGCTGGG AGAAGTGTGT TCTCCGCCAT GAAGCTCGGC 
AAGAACCGGT CCCACAAGGA GGAACCCCAA AGACAAGATG AACCGGCCGT GCTGGAGATG 
GAGGACCTTG ACCGCTTGGC CGTTCGGCTG GGGGATGGGC TGGACCCTGA CTCTGTGTCT 
CTGGCCTCTG TCACAGCTCT CACCACCAAT GTCTCCAACA AACGATCCAA GCCAGACATT 
AAGATGGTGC CAGGTGCCGG GCGGCCCGTG GATTACCAGG TCAGCATCAC CGTGATTGAG 
GCTCGGCAGC TCGTGGGCTT GAACATGGAC CCCGTGGTGT GCGTGGAGGT GGGCGACGAC 
AAGAAGTACA CATCCATGAA GGAGTCCACT AACTGCCCCT ACTACAATGA GTACTTCGTC 
TTCGACTTCC ACGTCTCTCC GGACGTCATG TTCGACAAGA TCATCAAGAT CTCGGTGATT 
CACTCCAAGA ACCTGCTTCG AAGCGGTACC CTGGTGGGGT CCTTCAAGAT GGATGTGGGA 
ACTGTGTACT CCCAGCCTGA GCACCAGTTC CACCACAAGT GGGCCATCCT GTCGGACCCC 
GACGACATCT CCGCCGGGCT GAAGGGCTAC GTGAAGTGTG ACGTGGCCGT GGTGGGCAAG 
GGGGACAACA TCAAGACGCC CCACAAGGCC AACGAGACAG ACGAGGATGA CATTGAAGGG 
AACTTGCTGC TCCCCGAGGG GGTGCCCCCC GAACGCCAGT GGGCCCGGTT CTACGTGAAG 
ATTTACCGAG CAGAAGGGCT GCCCCGTATG AACACCAGCC TCATGGCCAA TGTGAAGAAG 
GCGTTCATCG GGGAGAACAA GGACCTGGTT GACCCCTACG TGCAAGTCTT CTTTGCCGGC 
CAGAAGGGCA AGACATCAGT ACAGAAAAGC AGCTACGAGC CACTGTGGAA TGAGCAGGTC 
GTCTTTACGG ACCTGTTCCC CCCCCTCTGC AAGCGCATGA AGGTGCAGAT CCGAGATGCC 
GACAAGGTCA ACGACGTGGC CATCGGCACC CACTTCATTG ACCTGCGCAA GATTTCTAAT 
GATGGAGACA AAGGCTTCCT GCCCACGCTG GGCCCGGCCT GGGTGAACAT GTACGGCTCT 
ACACGCAACT ACACGCTGCT GGACGAGCAC CAGGACCTGA ATGAGGGCCT GGGGGAGGGC 
GTGTCGTTCC GCGCCCGCCT CATGCTGGGC CTGGCGGTGG AGATCCTGGA CACCTCCAAT 
CCTGAGCTGA CCAGCTCCAC GGAGGTGCAG GTGGAGCAGG CCACGCCTGT CTCGGAGAGC 
TGCACGGGGA AAATGGAAGA ATTCTTTCTA TTCGGAGCCT TCCTAGAGGC CTCAATGATC 
GACCGGAAAA ATGGCGACAA GCCAATCACC TTTGAGGTGA CCATAGGCAA CTATGGGAAT 
GAAGTTGATG GCCTGTCGCG GCCCCAGCGG CCTAGGCCCC GGAAGGAGCC TGGGGATGAG 
GAAGAAGTAG ATCTGATCCA GAACTCCAGT GATGAAGATG CCGGTGAGGG TGGGGACCTG 
GCCTCGGTGT CCTCGACTCC GCCCATGCGG CCCCAGATCA CCGACAGGAA CTACTTCCAT 
CTGCCCTACC TGGAGCGCAA GCCCTGCATC TACATCAAGA GCTGGTGGCC CGACCAGCGT 
CGCCGCCTCT ACAACGCCAA CATCATGGAC CACATCGCGG ACAAGCTGGA AGAAGGCCTG 
AACGACGTGC AGGAGATGAT AAAGACAGAG AAGTCCTATC CGGAGCGTCG CCTGCGGGGT 
GTCCTGGAGG AGCTCAGCTG TGGCTGCTAC CGCTTCCTCT CCCTCGCTGA CAAGGACCAG 
GGCCACTCGT CCCGCACCAG GCTGGACAGG GAGCGCCTCA AGTCCTGCAT GCGGGAGCTG 
GAGAGTATGG GGCAGCAGGC CAAGACCTTG CGGGCACAGG TGAAGCGGCA CACGGTGCGG 
GACAAGCTGA GGCTGTGCCA GAATTTCCTG CAGAAACTGC GCTTCCTGGC GGACGAGCCT 
CAGCACAGCA TCCCCGACGT CTTCATCTGG ATGATGAACA ACAACAAGCG CACCGCCTAC 
GCCAGGGTGC CCTCCAAGGA CCTGCTCTTC TCCATCGTTG AGGAGGAGCT GGGCAAGGAC 
TGCGCCAAGG TCAAGACGCT CTTCCTCAAG CTGCCGGGCA AGCGGGGCTT CGGCTCCGTG 
GGCTGGACGG TGCAGGCCAA GCTGGAGCTC TACCTGTGGC TGGGCCTGAG CAAGCAGCGC 
AAGGACTTCC TGTGCGGCCT GCCCTGCGGC TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG 
GGCCTGCACG CCTTCCCGCC CATCAGCCTG GTCTACACCA AGAAGCAGGC GTTCCAGCTC 
CGGGCCCACA TGTACCAGGC TCGCAGCCTC TTCGCTGCCG ACAGCAGCGG CCTCTCGGAC 
CCCTTCGCCC GCGTCTTCTT CATCAACCAG AGTCAGTGCA CGGAGGTGCT GAATGAGACC 
CTGTGCCCCA CCTGGGACCA GATGCTGGTG TTCGACAACC TGGAGCTATA TGGCGAAGCC 
CATGAGTTGC GGGATGACCC CCCCATCATC GTCATCGAAA TCTACGACCA GGACACCATG 
GGCAAAGCCG ACTTCATGGG CCGGACCTTC GCCAAGCCCC TGGTGAAGAT GGCGGACGAG 
GCGTACTGTC CACCCCGCTT CCCACCCCAG CTCGAGTACT ACCAGATCTA CCGCGGCAAC 
GCCACTGCCG GGGACCTGCT GGCTGCCTTC GAGTTGCTGC AGATTGGGCC TGCGGGGAAG 
GCAGACCTGC CACCCATCAA CGGCCCGGGG GACATGGACC GAGGTCCCAT CATGCCTGTG 
CCCGTCGGCA TCCGGCCCGT GCTCAGCAAA TACCGAATCG AGGTGCTCTT CTGGGGCCTG 
CGGGACCTGA AGCGGGTCAA CCTGGCCCAG GTGGACCGGC CGCGGGTAGA CATCGAGTGT 
GCCGGGAAGG GGGTGCAGTC GTCCCTGATC CACAATTACA AGAAGAACCC CAACTTCAAC 
ACCCTCGTCA AGTGGTTTGA AGTGGACCTC CCGGAGAACG AGCTGCTGCA CCCGCCCCTG 
AACATCCGAG TGGTGGACTG CCGGGCCTTC GGCCGCTACA CCCTGGTGGG TTCCCACGCC 
GTCAGCTCTC TGAGGCGCTT CATCTACAGG CCCCCAGACC GCTCGGCGCC CAACTGGAAC 
ACCACGGGCA GGCTCCCTCG GGGTCGCCGT GTGCTGTGCG ATGGCGGTCC CTCCTCTCAC 
CCCCCAGGGG AGGTCGTGGT GAACATGGAG CCAGAGGTGC CTGTCCGGAA GCTGGAGACC 
ATGGTGAAGC TGGACGCGAC TTCTGATGCC GTCATCAAGG TGGATGTGGC TGACGAGGAG 
AAAGAGAAGA AGAAAAAGAA GAAAAAGGGC AGCTCAGAAG AGCCAGAGGA GGAGGAGCCC 
GATGAGAGCA TGCTGGACTG GTGGTCCAAG TACTTTGCCT CCATCGATAC CATGAAGGAG 
CAACTTCGAC AGCAAGAGGC CTCCGGCATT GACTTGGAGG AGAAGGAGGA GGTGGAGAAC 
ACGGAGGGCC TAAAGGGGCC GATGAAGGGC AAGGAGAAGG CAAAGGCGGC GAAGGAGGAG 
AAGAAAAAGA GAACCCAGAG CCCCGGCACT GGCCAGGGGC CTGAGGCCCC AGAGAAGAAG 
AAACCCAAGA TTGATGAGCT TAAGGTGTAC CCCAAGGAGC TGGAGTCTGA GTTTGACAAC 
TTTGAGGACT GGCTGCACAC TTTCAATCTG CTGCGGGGCA AGACGGGGGA TGACGAGGAC 
GGTTCCACGG AGGAGGAGCG CATCGTGGGC CGGTTCAAGG GCTCCCTCTG CGTGTATAAA 
GTGCCACTCC CAGAGGACGT GTCCCGGGAA GCCGGCTACG ACCCCACCTA CGGCATGTTC 
CAGGGCATCC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATGT GGTCAGGGCC 
ACGGACCTGC ACCCCGCAGA CATTAACGGC AAAGCCGACC CCTACATTGC CATCCGGCTG 
GGCAAAACTG ACATCCGAGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCTGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCCATGGAAT CCATGCTGAC AGTGGCCGTG 
TACGACTGGG ATCTGGTAGG CACCGATGAC CTCATCGGGG AAACCAAGAT TGACCTGGAG 
AACCGCTTCT ACAGCAAACA CCGGGCCACC TGTGGCATCG CCCAGACCTA CTCCATACAT 
GGCTACAATA TCTGGCGGGA CCCCATGAAG CCCAGCCAGA TTCTGACCCG CCTCTGCAAG 
GAGGGCAAAG TGGACGGCCC CCACTTTGGG CCCCCCGGGA GAGTGAAGGT GTCCAACCGT 
GTCTTCACTG GGCCCTCCGA GATCGAGGAC GAGAATGGTC AGAGGAAGCC TACGGAAGAG 
CATGTGGCTC TATCTGCCCT GAGGCACTGG GAGGACATCC CCCGCGTGGG CTGCCGCCTG 
GTGCCCGAAC ACGTGGAAAC CCGGCCCCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGCCGCCTGG AGCTGTGGGT GGACATGTTC CCCATGGACA TGCCGGCCCC TGGGACGCCT 
CTGGACATCT CCCCCCGGAA GCCCAAGAAG TATGAGCTCC GGGTCATCGT GTGGAACACA 
GACGAGGTGG TCCTGGAGGA CGATGACTTC TTTACTGGGG AGAAGTCCAG CGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAGCAGG ACACGGACGT CCACTATCAT 
TCCCTCACCG GCGAGGGCAA CTTCAACTGG CGCTACCTGT TCCCCTTCGA CTACCTGGCC 
GCCGAGGAAA GGATTGTCAT CTCCAAGAAG GAGTCCATGT TCTCCTGGGA CGAGACCGAG 
TACAAGATCC CCGCGCGGCT CACCCTGCAG ATCTGGGACG CGGACCACTT CTCCGCGGAC 
GACTTCCTGG GGGCCATCGA GCTGGACCTC AACCGCTTTC CCCGGGGCGC CAAGACAGCC 
AAGCAGTGCA CCATGGAGAT GGCCACGGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTC 
AAGCAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTC 
GAGCTCACGG GCAAGGTGGA GGCAGAATTG CATCTACTGA CAGCGGAGGA GGCAGAGAAG 
AATCCGGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AGAAACCCAA CCGGCCTGAC 
ACCGCCTTCG TCTGGTTCCT CAACCCCCTC AAGTCCATCA AGTACCTCAT CTGCACGCGG 
TACAAGTGGC TCATCATCAA GATCGTGCTG GCACTGCTGG GGCTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCCGG CTACATGGTC AAGAAGCTCC TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_023252024.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023107792.1 Felis catus otoferlin (OTOF), transcript variant X4, mRNA.

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CloneID	OFb73954
Clone ID Related Accession (Same CDS sequence)	XM_023252024.1
Accession Version	XM_023252024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	otoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGCAGGCT CCCTCGGGGT CGCCGTGTGC TGTGCGATGG CGGTCCCTCC 
TCTCACCCCC CAGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAAGCGG 
CCAGACACGA GCTTCTTCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCCTGTGG 
CACACAAGCC GCTGGCGTTT CCTCAAACTG CTCCTCCTCC TGGCCCTGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107792.1
CDS	76..6072
Translation	MALFVHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENREDVADFDETFRWPVASSIDSNEILEIQIFNYSKVFSNKLIGTFRMVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGRLPRGRRVLCDGGPSSHPPGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPKRPDTSFFWFLNPLKSARYFLWHTSRWRFLKLLLLLALLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_023252024.1
Organism	Felis catus(domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023252024.1

ORF Insert Sequence:

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGA GCTGGGAGAA GTGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGTCCCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC GGCCGTGCTG 
GAGATGGAGG ACCTTGACCG CTTGGCCGTT CGGCTGGGGG ATGGGCTGGA CCCTGACTCT 
GTGTCTCTGG CCTCTGTCAC AGCTCTCACC ACCAATGTCT CCAACAAACG ATCCAAGCCA 
GACATTAAGA TGGTGCCAGG TGCCGGGCGG CCCGTGGATT ACCAGGTCAG CATCACCGTG 
ATTGAGGCTC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC 
TTCGTCTTCG ACTTCCACGT CTCTCCGGAC GTCATGTTCG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTTCGAAGC GGTACCCTGG TGGGGTCCTT CAAGATGGAT 
GTGGGAACTG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCG 
GACCCCGACG ACATCTCCGC CGGGCTGAAG GGCTACGTGA AGTGTGACGT GGCCGTGGTG 
GGCAAGGGGG ACAACATCAA GACGCCCCAC AAGGCCAACG AGACAGACGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATTT ACCGAGCAGA AGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG 
AAGAAGGCGT TCATCGGGGA GAACAAGGAC CTGGTTGACC CCTACGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC ATCAGTACAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTCGTCT TTACGGACCT GTTCCCCCCC CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GATGCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC 
GGCTCTACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGG 
GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAATCCTG AGCTGACCAG CTCCACGGAG GTGCAGGTGG AGCAGGCCAC GCCTGTCTCG 
GAGAGCTGCA CGGGGAAAAT GGAAGAATTC TTTCTATTCG GAGCCTTCCT AGAGGCCTCA 
ATGATCGACC GGAAAAATGG CGACAAGCCA ATCACCTTTG AGGTGACCAT AGGCAACTAT 
GGGAATGAAG TTGATGGCCT GTCGCGGCCC CAGCGGCCTA GGCCCCGGAA GGAGCCTGGG 
GATGAGGAAG AAGTAGATCT GATCCAGAAC TCCAGTGATG AAGATGCCGG TGAGGGTGGG 
GACCTGGCCT CGGTGTCCTC GACTCCGCCC ATGCGGCCCC AGATCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCCGAC 
CAGCGTCGCC GCCTCTACAA CGCCAACATC ATGGACCACA TCGCGGACAA GCTGGAAGAA 
GGCCTGAACG ACGTGCAGGA GATGATAAAG ACAGAGAAGT CCTATCCGGA GCGTCGCCTG 
CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCGTCCCG CACCAGGCTG GACAGGGAGC GCCTCAAGTC CTGCATGCGG 
GAGCTGGAGA GTATGGGGCA GCAGGCCAAG ACCTTGCGGG CACAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAT TTCCTGCAGA AACTGCGCTT CCTGGCGGAC 
GAGCCTCAGC ACAGCATCCC CGACGTCTTC ATCTGGATGA TGAACAACAA CAAGCGCACC 
GCCTACGCCA GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CGGGCAAGCG GGGCTTCGGC 
TCCGTGGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTGAGCAAG 
CAGCGCAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCACGCCTT CCCGCCCATC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGGG CCCACATGTA CCAGGCTCGC AGCCTCTTCG CTGCCGACAG CAGCGGCCTC 
TCGGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACGGA GGTGCTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTATATGGC 
GAAGCCCATG AGTTGCGGGA TGACCCCCCC ATCATCGTCA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCG 
GACGAGGCGT ACTGTCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAACGCCA CTGCCGGGGA CCTGCTGGCT GCCTTCGAGT TGCTGCAGAT TGGGCCTGCG 
GGGAAGGCAG ACCTGCCACC CATCAACGGC CCGGGGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCCG TCGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG 
GGCCTGCGGG ACCTGAAGCG GGTCAACCTG GCCCAGGTGG ACCGGCCGCG GGTAGACATC 
GAGTGTGCCG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCGG AGAACGAGCT GCTGCACCCG 
CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGTTCC 
CACGCCGTCA GCTCTCTGAG GCGCTTCATC TACAGGCCCC CAGACCGCTC GGCGCCCAAC 
TGGAACACCA CGGGCAGGCT CCCTCGGGGT CGCCGTGTGC TGTGCGATGG CGGTCCCTCC 
TCTCACCCCC CAGGGGAGGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CCGGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCCGTCA TCAAGGTGGA TGTGGCTGAC 
GAGGAGAAAG AGAAGAAGAA AAAGAAGAAA AAGGGCAGCT CAGAAGAGCC AGAGGAGGAG 
GAGCCCGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGATACCATG 
AAGGAGCAAC TTCGACAGCA AGAGGCCTCC GGCATTGACT TGGAGGAGAA GGAGGAGGTG 
GAGAACACGG AGGGCCTAAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAA GGCGGCGAAG 
GAGGAGAAGA AAAAGAGAAC CCAGAGCCCC GGCACTGGCC AGGGGCCTGA GGCCCCAGAG 
AAGAAGAAAC CCAAGATTGA TGAGCTTAAG GTGTACCCCA AGGAGCTGGA GTCTGAGTTT 
GACAACTTTG AGGACTGGCT GCACACTTTC AATCTGCTGC GGGGCAAGAC GGGGGATGAC 
GAGGACGGTT CCACGGAGGA GGAGCGCATC GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TATAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAACGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC 
AGGGCCACGG ACCTGCACCC CGCAGACATT AACGGCAAAG CCGACCCCTA CATTGCCATC 
CGGCTGGGCA AAACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGGA AGTCCTTTGA CATCGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACAGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACC GATGACCTCA TCGGGGAAAC CAAGATTGAC 
CTGGAGAACC GCTTCTACAG CAAACACCGG GCCACCTGTG GCATCGCCCA GACCTACTCC 
ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGTGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCTACG 
GAAGAGCATG TGGCTCTATC TGCCCTGAGG CACTGGGAGG ACATCCCCCG CGTGGGCTGC 
CGCCTGGTGC CCGAACACGT GGAAACCCGG CCCCTGCTCA ACCCTGACAA GCCAGGCATC 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTCCCCA TGGACATGCC GGCCCCTGGG 
ACGCCTCTGG ACATCTCCCC CCGGAAGCCC AAGAAGTATG AGCTCCGGGT CATCGTGTGG 
AACACAGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC 
ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGACGTCCAC 
TATCATTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCCGCCG AGGAAAGGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAGTACA AGATCCCCGC GCGGCTCACC CTGCAGATCT GGGACGCGGA CCACTTCTCC 
GCGGACGACT TCCTGGGGGC CATCGAGCTG GACCTCAACC GCTTTCCCCG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAATTGCATC TACTGACAGC GGAGGAGGCA 
GAGAAGAATC CGGTGGGCCT GGCCCGCAAT GAACCTGACC CTCTAGAGAA ACCCAAGCGG 
CCAGACACGA GCTTCTTCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCCTGTGG 
CACACAAGCC GCTGGCGTTT CCTCAAACTG CTCCTCCTCC TGGCCCTGCT GCTCATGCTG 
GCCCTCTTCC TCTACAGCCT CCCCGGCTAC ATGGTCAAGA AGCTCCTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_019827789.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019683348.1 Felis catus otoferlin (OTOF), transcript variant X1, mRNA.

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CloneID	OFb40724
Clone ID Related Accession (Same CDS sequence)	XM_019827789.1
Accession Version	XM_019827789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	otoferlin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC ACAAAGCTGG GAGAAGTGTG TTCTCCGCCA TGAAGCTCGG CAAGAACCGG 
TCCCACAAGG AGGAACCCCA AAGACAAGAT GAACCGGCCG TGCTGGAGAT GGAGGACCTT 
GACCGCTTGG CCGTTCGGCT GGGGGATGGG CTGGACCCTG ACTCTGTGTC TCTGGCCTCT 
GTCACAGCTC TCACCACCAA TGTCTCCAAC AAACGATCCA AGCCAGACAT TAAGATGGTG 
CCAGGTGCCG GGCGGCCCGT GGATTACCAG GTCAGCATCA CCGTGATTGA GGCTCGGCAG 
CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGCGACGA CAAGAAGTAC 
ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTCGT CTTCGACTTC 
CACGTCTCTC CGGACGTCAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG 
AACCTGCTTC GAAGCGGTAC CCTGGTGGGG TCCTTCAAGA TGGATGTGGG AACTGTGTAC 
TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCGGACCC CGACGACATC 
TCCGCCGGGC TGAAGGGCTA CGTGAAGTGT GACGTGGCCG TGGTGGGCAA GGGGGACAAC 
ATCAAGACGC CCCACAAGGC CAACGAGACA GACGAGGATG ACATTGAAGG GAACTTGCTG 
CTCCCCGAGG GGGTGCCCCC CGAACGCCAG TGGGCCCGGT TCTACGTGAA GATTTACCGA 
GCAGAAGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCGTTCATC 
GGGGAGAACA AGGACCTGGT TGACCCCTAC GTGCAAGTCT TCTTTGCCGG CCAGAAGGGC 
AAGACATCAG TACAGAAAAG CAGCTACGAG CCACTGTGGA ATGAGCAGGT CGTCTTTACG 
GACCTGTTCC CCCCCCTCTG CAAGCGCATG AAGGTGCAGA TCCGAGATGC CGACAAGGTC 
AACGACGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCTAA TGATGGAGAC 
AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC TACACGCAAC 
TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGGGAGGG CGTGTCGTTC 
CGCGCCCGCC TCATGCTGGG CCTGGCGGTG GAGATCCTGG ACACCTCCAA TCCTGAGCTG 
ACCAGCTCCA CGGAGGTGCA GGTGGAGCAG GCCACGCCTG TCTCGGAGAG CTGCACGGGG 
AAAATGGAAG AATTCTTTCT ATTCGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA 
AATGGCGACA AGCCAATCAC CTTTGAGGTG ACCATAGGCA ACTATGGGAA TGAAGTTGAT 
GGCCTGTCGC GGCCCCAGCG GCCTAGGCCC CGGAAGGAGC CTGGGGATGA GGAAGAAGTA 
GATCTGATCC AGAACTCCAG TGATGAAGAT GCCGGTGAGG GTGGGGACCT GGCCTCGGTG 
TCCTCGACTC CGCCCATGCG GCCCCAGATC ACCGACAGGA ACTACTTCCA TCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATCAAG AGCTGGTGGC CCGACCAGCG TCGCCGCCTC 
TACAACGCCA ACATCATGGA CCACATCGCG GACAAGCTGG AAGAAGGCCT GAACGACGTG 
CAGGAGATGA TAAAGACAGA GAAGTCCTAT CCGGAGCGTC GCCTGCGGGG TGTCCTGGAG 
GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCTG ACAAGGACCA GGGCCACTCG 
TCCCGCACCA GGCTGGACAG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAGAGTATG 
GGGCAGCAGG CCAAGACCTT GCGGGCACAG GTGAAGCGGC ACACGGTGCG GGACAAGCTG 
AGGCTGTGCC AGAATTTCCT GCAGAAACTG CGCTTCCTGG CGGACGAGCC TCAGCACAGC 
ATCCCCGACG TCTTCATCTG GATGATGAAC AACAACAAGC GCACCGCCTA CGCCAGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGCAAGGA CTGCGCCAAG 
GTCAAGACGC TCTTCCTCAA GCTGCCGGGC AAGCGGGGCT TCGGCTCCGT GGGCTGGACG 
GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTGA GCAAGCAGCG CAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC 
GCCTTCCCGC CCATCAGCCT GGTCTACACC AAGAAGCAGG CGTTCCAGCT CCGGGCCCAC 
ATGTACCAGG CTCGCAGCCT CTTCGCTGCC GACAGCAGCG GCCTCTCGGA CCCCTTCGCC 
CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACGGAGGTGC TGAATGAGAC CCTGTGCCCC 
ACCTGGGACC AGATGCTGGT GTTCGACAAC CTGGAGCTAT ATGGCGAAGC CCATGAGTTG 
CGGGATGACC CCCCCATCAT CGTCATCGAA ATCTACGACC AGGACACCAT GGGCAAAGCC 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCGGACGA GGCGTACTGT 
CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA CGCCACTGCC 
GGGGACCTGC TGGCTGCCTT CGAGTTGCTG CAGATTGGGC CTGCGGGGAA GGCAGACCTG 
CCACCCATCA ACGGCCCGGG GGACATGGAC CGAGGTCCCA TCATGCCTGT GCCCGTCGGC 
ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GCGGGACCTG 
AAGCGGGTCA ACCTGGCCCA GGTGGACCGG CCGCGGGTAG ACATCGAGTG TGCCGGGAAG 
GGGGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC 
AAGTGGTTTG AAGTGGACCT CCCGGAGAAC GAGCTGCTGC ACCCGCCCCT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GTTCCCACGC CGTCAGCTCT 
CTGAGGCGCT TCATCTACAG GCCCCCAGAC CGCTCGGCGC CCAACTGGAA CACCACGGGC 
AGGCTCCCTC GGGGTCGCCG TGTGCTGTGC GATGGCGGTC CCTCCTCTCA CCCCCCAGGG 
GAGGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCCGGA AGCTGGAGAC CATGGTGAAG 
CTGGACGCGA CTTCTGATGC CGTCATCAAG GTGGATGTGG CTGACGAGGA GAAAGAGAAG 
AAGAAAAAGA AGAAAAAGGG CAGCTCAGAA GAGCCAGAGG AGGAGGAGCC CGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGATA CCATGAAGGA GCAACTTCGA 
CAGCAAGAGG CCTCCGGCAT TGACTTGGAG GAGAAGGAGG AGGTGGAGAA CACGGAGGGC 
CTAAAGGGGC CGATGAAGGG CAAGGAGAAG GCAAAGGCGG CGAAGGAGGA GAAGAAAAAG 
AGAACCCAGA GCCCCGGCAC TGGCCAGGGG CCTGAGGCCC CAGAGAAGAA GAAACCCAAG 
ATTGATGAGC TTAAGGTGTA CCCCAAGGAG CTGGAGTCTG AGTTTGACAA CTTTGAGGAC 
TGGCTGCACA CTTTCAATCT GCTGCGGGGC AAGACGGGGG ATGACGAGGA CGGTTCCACG 
GAGGAGGAGC GCATCGTGGG CCGGTTCAAG GGCTCCCTCT GCGTGTATAA AGTGCCACTC 
CCAGAGGACG TGTCCCGGGA AGCCGGCTAC GACCCCACCT ACGGCATGTT CCAGGGCATC 
CCCAGCAACG ACCCCATCAA TGTGCTGGTC CGAGTCTATG TGGTCAGGGC CACGGACCTG 
CACCCCGCAG ACATTAACGG CAAAGCCGAC CCCTACATTG CCATCCGGCT GGGCAAAACT 
GACATCCGAG ACAAGGAGAA CTACATCTCC AAGCAGCTCA ACCCTGTCTT TGGGAAGTCC 
TTTGACATCG AGGCCTCCTT CCCCATGGAA TCCATGCTGA CAGTGGCCGT GTACGACTGG 
GATCTGGTAG GCACCGATGA CCTCATCGGG GAAACCAAGA TTGACCTGGA GAACCGCTTC 
TACAGCAAAC ACCGGGCCAC CTGTGGCATC GCCCAGACCT ACTCCATACA TGGCTACAAT 
ATCTGGCGGG ACCCCATGAA GCCCAGCCAG ATTCTGACCC GCCTCTGCAA GGAGGGCAAA 
GTGGACGGCC CCCACTTTGG GCCCCCCGGG AGAGTGAAGG TGTCCAACCG TGTCTTCACT 
GGGCCCTCCG AGATCGAGGA CGAGAATGGT CAGAGGAAGC CTACGGAAGA GCATGTGGCT 
CTATCTGCCC TGAGGCACTG GGAGGACATC CCCCGCGTGG GCTGCCGCCT GGTGCCCGAA 
CACGTGGAAA CCCGGCCCCT GCTCAACCCT GACAAGCCAG GCATCGAGCA GGGCCGCCTG 
GAGCTGTGGG TGGACATGTT CCCCATGGAC ATGCCGGCCC CTGGGACGCC TCTGGACATC 
TCCCCCCGGA AGCCCAAGAA GTATGAGCTC CGGGTCATCG TGTGGAACAC AGACGAGGTG 
GTCCTGGAGG ACGATGACTT CTTTACTGGG GAGAAGTCCA GCGACATTTT TGTGCGGGGG 
TGGCTGAAGG GCCAGCAGGA GGACAAGCAG GACACGGACG TCCACTATCA TTCCCTCACC 
GGCGAGGGCA ACTTCAACTG GCGCTACCTG TTCCCCTTCG ACTACCTGGC CGCCGAGGAA 
AGGATTGTCA TCTCCAAGAA GGAGTCCATG TTCTCCTGGG ACGAGACCGA GTACAAGATC 
CCCGCGCGGC TCACCCTGCA GATCTGGGAC GCGGACCACT TCTCCGCGGA CGACTTCCTG 
GGGGCCATCG AGCTGGACCT CAACCGCTTT CCCCGGGGCG CCAAGACAGC CAAGCAGTGC 
ACCATGGAGA TGGCCACGGG GGAGGTGGAC GTGCCCCTGG TTTCCATCTT CAAGCAGAAG 
CGCGTCAAAG GCTGGTGGCC CCTCCTGGCC CGCAATGAGA ATGATGAGTT CGAGCTCACG 
GGCAAGGTGG AGGCAGAATT GCATCTACTG ACAGCGGAGG AGGCAGAGAA GAATCCGGTG 
GGCCTGGCCC GCAATGAACC TGACCCTCTA GAGAAACCCA ACCGGCCTGA CACCGCCTTC 
GTCTGGTTCC TCAACCCCCT CAAGTCCATC AAGTACCTCA TCTGCACGCG GTACAAGTGG 
CTCATCATCA AGATCGTGCT GGCGCTGCTG GGGCTGCTCA TGCTGGCCCT CTTCCTCTAC 
AGCCTCCCCG GCTACATGGT CAAGAAGCTC CTGGGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019683348.1
CDS	76..6117
Translation	MALFVHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENREDVADFDETFRWPVASSIDSNEILEIQIFNYSKVFSNKLIGTFRMVLQKVVEEKRVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLHEEEKDSQETDGLLPGSRPSSRTPGEKSFRSKGREKTKGGRDGEHKAGRSVFSAMKLGKNRSHKEEPQRQDEPAVLEMEDLDRLAVRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMVPGAGRPVDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDADKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGKMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNSSDEDAGEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMNNNKRTAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSVGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHAFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPGDMDRGPIMPVPVGIRPVLSKYRIEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGRLPRGRRVLCDGGPSSHPPGEVVVNMEPEVPVRKLETMVKLDATSDAVIKVDVADEEKEKKKKKKKGSSEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEASGIDLEEKEEVENTEGLKGPMKGKEKAKAAKEEKKKRTQSPGTGQGPEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRVFTGPSEIEDENGQRKPTEEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_019827789.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus otoferlin (OTOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827789.1

ORF Insert Sequence:

ATGGCCCTGT TTGTCCACCT CAAGACGGTT TCGGAGCTTC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TGCTGGAGAA CCGTGAAGAT 
GTGGCTGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAGCATTGA CAGCAATGAG 
ATACTCGAGA TTCAGATCTT CAACTATAGC AAAGTCTTCA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAAGCGTG TGGAGGTGAC CGACACACTG 
ATCGATGACA ACAACGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG ATATCAGGCC 
ACAGATGGCA CGGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGGGATGA GTCCCTTCAT 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGACTGCTCC CCGGCTCCCG CCCCAGCTCC 
CGGACACCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC ACAAAGCTGG GAGAAGTGTG TTCTCCGCCA TGAAGCTCGG CAAGAACCGG 
TCCCACAAGG AGGAACCCCA AAGACAAGAT GAACCGGCCG TGCTGGAGAT GGAGGACCTT 
GACCGCTTGG CCGTTCGGCT GGGGGATGGG CTGGACCCTG ACTCTGTGTC TCTGGCCTCT 
GTCACAGCTC TCACCACCAA TGTCTCCAAC AAACGATCCA AGCCAGACAT TAAGATGGTG 
CCAGGTGCCG GGCGGCCCGT GGATTACCAG GTCAGCATCA CCGTGATTGA GGCTCGGCAG 
CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGCGACGA CAAGAAGTAC 
ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTCGT CTTCGACTTC 
CACGTCTCTC CGGACGTCAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG 
AACCTGCTTC GAAGCGGTAC CCTGGTGGGG TCCTTCAAGA TGGATGTGGG AACTGTGTAC 
TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCGGACCC CGACGACATC 
TCCGCCGGGC TGAAGGGCTA CGTGAAGTGT GACGTGGCCG TGGTGGGCAA GGGGGACAAC 
ATCAAGACGC CCCACAAGGC CAACGAGACA GACGAGGATG ACATTGAAGG GAACTTGCTG 
CTCCCCGAGG GGGTGCCCCC CGAACGCCAG TGGGCCCGGT TCTACGTGAA GATTTACCGA 
GCAGAAGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCGTTCATC 
GGGGAGAACA AGGACCTGGT TGACCCCTAC GTGCAAGTCT TCTTTGCCGG CCAGAAGGGC 
AAGACATCAG TACAGAAAAG CAGCTACGAG CCACTGTGGA ATGAGCAGGT CGTCTTTACG 
GACCTGTTCC CCCCCCTCTG CAAGCGCATG AAGGTGCAGA TCCGAGATGC CGACAAGGTC 
AACGACGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCTAA TGATGGAGAC 
AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC TACACGCAAC 
TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGGGAGGG CGTGTCGTTC 
CGCGCCCGCC TCATGCTGGG CCTGGCGGTG GAGATCCTGG ACACCTCCAA TCCTGAGCTG 
ACCAGCTCCA CGGAGGTGCA GGTGGAGCAG GCCACGCCTG TCTCGGAGAG CTGCACGGGG 
AAAATGGAAG AATTCTTTCT ATTCGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA 
AATGGCGACA AGCCAATCAC CTTTGAGGTG ACCATAGGCA ACTATGGGAA TGAAGTTGAT 
GGCCTGTCGC GGCCCCAGCG GCCTAGGCCC CGGAAGGAGC CTGGGGATGA GGAAGAAGTA 
GATCTGATCC AGAACTCCAG TGATGAAGAT GCCGGTGAGG GTGGGGACCT GGCCTCGGTG 
TCCTCGACTC CGCCCATGCG GCCCCAGATC ACCGACAGGA ACTACTTCCA TCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATCAAG AGCTGGTGGC CCGACCAGCG TCGCCGCCTC 
TACAACGCCA ACATCATGGA CCACATCGCG GACAAGCTGG AAGAAGGCCT GAACGACGTG 
CAGGAGATGA TAAAGACAGA GAAGTCCTAT CCGGAGCGTC GCCTGCGGGG TGTCCTGGAG 
GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCTG ACAAGGACCA GGGCCACTCG 
TCCCGCACCA GGCTGGACAG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAGAGTATG 
GGGCAGCAGG CCAAGACCTT GCGGGCACAG GTGAAGCGGC ACACGGTGCG GGACAAGCTG 
AGGCTGTGCC AGAATTTCCT GCAGAAACTG CGCTTCCTGG CGGACGAGCC TCAGCACAGC 
ATCCCCGACG TCTTCATCTG GATGATGAAC AACAACAAGC GCACCGCCTA CGCCAGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGCAAGGA CTGCGCCAAG 
GTCAAGACGC TCTTCCTCAA GCTGCCGGGC AAGCGGGGCT TCGGCTCCGT GGGCTGGACG 
GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTGA GCAAGCAGCG CAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC 
GCCTTCCCGC CCATCAGCCT GGTCTACACC AAGAAGCAGG CGTTCCAGCT CCGGGCCCAC 
ATGTACCAGG CTCGCAGCCT CTTCGCTGCC GACAGCAGCG GCCTCTCGGA CCCCTTCGCC 
CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACGGAGGTGC TGAATGAGAC CCTGTGCCCC 
ACCTGGGACC AGATGCTGGT GTTCGACAAC CTGGAGCTAT ATGGCGAAGC CCATGAGTTG 
CGGGATGACC CCCCCATCAT CGTCATCGAA ATCTACGACC AGGACACCAT GGGCAAAGCC 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCGGACGA GGCGTACTGT 
CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA CGCCACTGCC 
GGGGACCTGC TGGCTGCCTT CGAGTTGCTG CAGATTGGGC CTGCGGGGAA GGCAGACCTG 
CCACCCATCA ACGGCCCGGG GGACATGGAC CGAGGTCCCA TCATGCCTGT GCCCGTCGGC 
ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GCGGGACCTG 
AAGCGGGTCA ACCTGGCCCA GGTGGACCGG CCGCGGGTAG ACATCGAGTG TGCCGGGAAG 
GGGGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC 
AAGTGGTTTG AAGTGGACCT CCCGGAGAAC GAGCTGCTGC ACCCGCCCCT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GTTCCCACGC CGTCAGCTCT 
CTGAGGCGCT TCATCTACAG GCCCCCAGAC CGCTCGGCGC CCAACTGGAA CACCACGGGC 
AGGCTCCCTC GGGGTCGCCG TGTGCTGTGC GATGGCGGTC CCTCCTCTCA CCCCCCAGGG 
GAGGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCCGGA AGCTGGAGAC CATGGTGAAG 
CTGGACGCGA CTTCTGATGC CGTCATCAAG GTGGATGTGG CTGACGAGGA GAAAGAGAAG 
AAGAAAAAGA AGAAAAAGGG CAGCTCAGAA GAGCCAGAGG AGGAGGAGCC CGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGATA CCATGAAGGA GCAACTTCGA 
CAGCAAGAGG CCTCCGGCAT TGACTTGGAG GAGAAGGAGG AGGTGGAGAA CACGGAGGGC 
CTAAAGGGGC CGATGAAGGG CAAGGAGAAG GCAAAGGCGG CGAAGGAGGA GAAGAAAAAG 
AGAACCCAGA GCCCCGGCAC TGGCCAGGGG CCTGAGGCCC CAGAGAAGAA GAAACCCAAG 
ATTGATGAGC TTAAGGTGTA CCCCAAGGAG CTGGAGTCTG AGTTTGACAA CTTTGAGGAC 
TGGCTGCACA CTTTCAATCT GCTGCGGGGC AAGACGGGGG ATGACGAGGA CGGTTCCACG 
GAGGAGGAGC GCATCGTGGG CCGGTTCAAG GGCTCCCTCT GCGTGTATAA AGTGCCACTC 
CCAGAGGACG TGTCCCGGGA AGCCGGCTAC GACCCCACCT ACGGCATGTT CCAGGGCATC 
CCCAGCAACG ACCCCATCAA TGTGCTGGTC CGAGTCTATG TGGTCAGGGC CACGGACCTG 
CACCCCGCAG ACATTAACGG CAAAGCCGAC CCCTACATTG CCATCCGGCT GGGCAAAACT 
GACATCCGAG ACAAGGAGAA CTACATCTCC AAGCAGCTCA ACCCTGTCTT TGGGAAGTCC 
TTTGACATCG AGGCCTCCTT CCCCATGGAA TCCATGCTGA CAGTGGCCGT GTACGACTGG 
GATCTGGTAG GCACCGATGA CCTCATCGGG GAAACCAAGA TTGACCTGGA GAACCGCTTC 
TACAGCAAAC ACCGGGCCAC CTGTGGCATC GCCCAGACCT ACTCCATACA TGGCTACAAT 
ATCTGGCGGG ACCCCATGAA GCCCAGCCAG ATTCTGACCC GCCTCTGCAA GGAGGGCAAA 
GTGGACGGCC CCCACTTTGG GCCCCCCGGG AGAGTGAAGG TGTCCAACCG TGTCTTCACT 
GGGCCCTCCG AGATCGAGGA CGAGAATGGT CAGAGGAAGC CTACGGAAGA GCATGTGGCT 
CTATCTGCCC TGAGGCACTG GGAGGACATC CCCCGCGTGG GCTGCCGCCT GGTGCCCGAA 
CACGTGGAAA CCCGGCCCCT GCTCAACCCT GACAAGCCAG GCATCGAGCA GGGCCGCCTG 
GAGCTGTGGG TGGACATGTT CCCCATGGAC ATGCCGGCCC CTGGGACGCC TCTGGACATC 
TCCCCCCGGA AGCCCAAGAA GTATGAGCTC CGGGTCATCG TGTGGAACAC AGACGAGGTG 
GTCCTGGAGG ACGATGACTT CTTTACTGGG GAGAAGTCCA GCGACATTTT TGTGCGGGGG 
TGGCTGAAGG GCCAGCAGGA GGACAAGCAG GACACGGACG TCCACTATCA TTCCCTCACC 
GGCGAGGGCA ACTTCAACTG GCGCTACCTG TTCCCCTTCG ACTACCTGGC CGCCGAGGAA 
AGGATTGTCA TCTCCAAGAA GGAGTCCATG TTCTCCTGGG ACGAGACCGA GTACAAGATC 
CCCGCGCGGC TCACCCTGCA GATCTGGGAC GCGGACCACT TCTCCGCGGA CGACTTCCTG 
GGGGCCATCG AGCTGGACCT CAACCGCTTT CCCCGGGGCG CCAAGACAGC CAAGCAGTGC 
ACCATGGAGA TGGCCACGGG GGAGGTGGAC GTGCCCCTGG TTTCCATCTT CAAGCAGAAG 
CGCGTCAAAG GCTGGTGGCC CCTCCTGGCC CGCAATGAGA ATGATGAGTT CGAGCTCACG 
GGCAAGGTGG AGGCAGAATT GCATCTACTG ACAGCGGAGG AGGCAGAGAA GAATCCGGTG 
GGCCTGGCCC GCAATGAACC TGACCCTCTA GAGAAACCCA ACCGGCCTGA CACCGCCTTC 
GTCTGGTTCC TCAACCCCCT CAAGTCCATC AAGTACCTCA TCTGCACGCG GTACAAGTGG 
CTCATCATCA AGATCGTGCT GGCGCTGCTG GGGCTGCTCA TGCTGGCCCT CTTCCTCTAC 
AGCCTCCCCG GCTACATGGT CAAGAAGCTC CTGGGGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	OTOF
Entrez Gene ID	101083509
Full Name	otoferlin
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

OTOF cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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