MED12 gene cDNA ORF clone, Equus caballus(horse)

mRNA	Protein	Name
XM_023633667.1	XP_023489435.1	mediator of RNA polymerase II transcription subunit 12 isoform X3
XM_023633672.1	XP_023489440.1	mediator of RNA polymerase II transcription subunit 12 isoform X7
XM_023633675.1	XP_023489443.1	mediator of RNA polymerase II transcription subunit 12 isoform X10
XM_005614273.1	XP_005614330.1	mediator of RNA polymerase II transcription subunit 12
XM_023633666.1	XP_023489434.1	mediator of RNA polymerase II transcription subunit 12 isoform X2
XM_023633665.1	XP_023489433.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_023633670.1	XP_023489438.1	mediator of RNA polymerase II transcription subunit 12 isoform X5
XM_023633671.1	XP_023489439.1	mediator of RNA polymerase II transcription subunit 12 isoform X6
XM_023633668.1	XP_023489436.1	mediator of RNA polymerase II transcription subunit 12 isoform X4
XM_023633673.1	XP_023489441.1	mediator of RNA polymerase II transcription subunit 12 isoform X8
XM_023633674.1	XP_023489442.1	mediator of RNA polymerase II transcription subunit 12 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb71705	XM_023633674.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb27102	XM_005614273.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71697	XM_023633665.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71698	XM_023633666.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71699	XM_023633667.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71700	XM_023633668.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71701	XM_023633670.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71702	XM_023633671.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71703	XM_023633672.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71704	XM_023633673.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb71706	XM_023633675.1 Latest version!	Equus caballus mediator complex subunit 12 (MED12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

MED12 ( XM_023633674.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489442.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHb71705
Clone ID Related Accession (Same CDS sequence)	XM_023633674.1
Accession Version	XM_023633674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGCGGGG GCTGTGGTTC CACTATAGGG 
CCCTTGCCCC ATGATGTAGA AGTGGCAATC CGGCAGTGGG ACTACAATGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTACTTGAG 
TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGTT GCCCCTGCTG 
CTTCGATACT CTGGGGAATT CGTTCAGTCT GCATACCTCT CTCGCCGCCT TGCCTACTTC 
TGTACCCGGA GGCTGGCCCT GCAGCTGGAT GGCGTGAGCA GTCACTCGTC TCATGTGATG 
TCTGCTCAGT CGACAGGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC GACTAGCAGC 
ACACCCTCTA CACCCTTTAG TGACCTACTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT 
GGCCTCAGCT GTATCCTTCA GACCATCCTC CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC 
TACTCACTGA CTGATAGCCG AATTAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC 
CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TTGAGCAGCA GATCAAGGAG CGAGGACAGG CAGTTGAGGT TCGCTGGTCT 
TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTGCT CCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCTTG 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TATCATTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAAAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCTAGTGCTC CCATTTTCCA GGATGTTCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CCCGAAGTGA GAGTGAGCGG GTGGAGTTCT TTAACTTGGT ACTGCTGTTC 
TGTGAACTCA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC CCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCT 
GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GTAGCAAGCT GGAGGATCCG 
GGGCTCTCGG AGTCTATGGA CATAGACCCT AGTTCCAGTG TACTGTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCTCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATAGAAGGG 
ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGACATGCC ATCAAGAAAA TAACCAAGGA TATCCTGAAG 
GTTCTGAACC GCAAGGGGAC GGCGGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGACGGCG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCATCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGTTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTACT GAATGAACTG 
AGTGTAGTTG AGGCTGAGTT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACC 
AGCCTGTGCC TGTGCATTGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTGAAGCA TGGGATGAAC 
CGGTCAGATG GCTCCTCTGC AGAACGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAGCTCTTCA GCGACTTCTG CTCCAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAATCCA ATATGCGCTG GGCACCTGAG 
TTCATGATTG ACACTCTGGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCG GAATGGCTAG GAGTCCTTAA AGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT 
GACCTGTCTT TTCATGATTC CCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTAG AGGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGC 
AGTGAGCAGG ATTCTGAGCC CGGGGCCCGA CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCTC AACTCAATCC TTGCCAGTCG GATGGAACTC CTTCCCCAGA CAAGCCTACT 
GTAGGAATCC GCTCCTCCTG TGACCGCCAC CTGCTGGCTG CCTCCCAGAA CCGCATCGTG 
GATGGAGCTG TGTTTGCTGT TCTCAAGGCT GTGTTTGTAC TTGGCGATGC GGAACTGAAG 
GGTTCAGGCT TCACTGTGAC AGGAGGAACA GAAGAACTTC CAGAGGAGGA GGGGGGAGGT 
GGCAGTGGCG GTCGGAGGCA GGGTGGCCGC AACATCTCTG TGGAGACAGC CAGTCTGGAT 
GTCTATGCCA AGTACGTGCT GCGCAGCATC TGCCAACAGG AATGGGTAGG AGAACGTTGC 
CTTAAATCGC TGTGTGAGGA CAGCAATGAC CTACAAGACC CAGTGTTGAG TAGCGCCCAG 
GCCCAGCGCC TCATGCAGCT CATCTGCTAC CCACATCGGC TGCTGGACAA TGAGGATGGG 
GAAAATCCCC AGCGACAACG CATTAAGCGT ATTCTCCAGA ACTTGGACCA GTGGACCATG 
CGTCAGTCTT CCCTGGAGCT GCAGCTCATG ATCAAGCAGA CCCCTAACAA TGAGATGAAC 
TCCCTCTTAG AGAACATTGC CAAGGCCACA ATCGAGGTGT TCCAACAGTC AGCAGAGACA 
GGGTCATCTT CTGGAAACAC TGCAAGCAAC ATGCCCAGCA GCAGCAAGAC CAAGCCTGTG 
CTCAGCTCTC TGGAGCGCTC TGGTGTATGG CTGGTGGCCC CCCTCATTGC TAAACTGCCC 
ACCTCAGTCC AGGGGCATGT GTTAAAGGCT GCTGGGGAGG AATTGGAGAA GGGCCAGCAC 
CTGGGTTCCT CTTCCCGCAA AGAACGTGAT CGACAAAAGC AAAAGAGTAT GTCCCTGTTG 
AGCCAGCAGC CTTTCTTGTC CCTGGTGCTG ACGTGTCTGA AAGGGCAGGA TGAGCAGCGT 
GAGGGACTCC TTACCTCCCT TTACAGCCAA GTGCACCAGA TTGTGAATAA TTGGCGAGAT 
GACCAGTACT TAGATGATTG CAAACCAAAG CAGCTAATGC ATGAGGCACT CAAACTGCGG 
CTCAACCTGG TGGGGGGCAT GTTTGACACG GTGCAGCGCA GCACCCAACA GACCACAGAG 
TGGGCTGTGC TCCTCCTGGA GATCATCATC AGCGGCACTG TCGACATGCA GTCCAACAAC 
GAGCTCTTCA CCACTGTATT GGACATGCTG AGCGTGCTCA TCAATGGGAC CCTGGCTGCG 
GACATGTCCA GCATCTCTCA AGGCAGCATG GAGGAAAACA AACGCGCCTA CATGAACCTG 
GTGAAGAAGC TGCGGAAAGA GTTGGGGGAG CGCCAATCAG ACAGTCTGGA AAAAGTTCGC 
CAGCTGCTGC CACTGCCCAA GCAGACCCGA GATGTCATCA CATGTGAGCC ACAGGGCTCC 
CTTATTGACA CCAAGGGCAA CAAGATTGCC GGCTTTGATT CCATCTTCAA GAAAGAGGGT 
CTACAGGTTT CCACCAAACA AAAGATCTCT CCCTGGGATC TTTTTGAGGG GCTGAAGCCG 
TCTGCACCAC TCTCTTGGGG CTGGTTTGGA ACAGTCCGGG TCGACAGGCG AGTGGCCCGA 
GGCGAGGAGC AGCAGCGGTT GCTGCTCTAC CACACACACC TGCGGCCCCG GCCCCGTGCC 
TATTACCTGG AGCCACTGCC ACTGCCACCA GAGGATGAGG AGCCCCCCGC TCCCACCCCG 
CTAGAGCCTG AGAAAAAGGC TCCAGAGCCC CCCAAAACTG ACAAGCCTGG GGCTGCTCCA 
CCTAGTACTG AGGAACGCAA GAAGAAGTCC ACGAAGGGCA AGAAACGCAG CCAGCCTGCT 
GCCAAGACAG AGGACTATGG AATGGGCCCA GGGCGGAGTG GCCCCTATGG TGTGACCGTG 
CCTCCGGACC TCCTGCACCA CGCCAACCCT GGTTCCATAT CCCACCTTAG CTACAGGCAG 
GGTTCCATAG GCCTGTACAC CCAGAACCAG CCACTACCTG CAGGTGGCCC TCGTGTGGAC 
CCATACCGCC CTATGCGGTT ACCAATGCAG AAGCTGCCGA CCCGACCACC TTACCCTGGA 
GTGCTACCCA CAGCCGTGAC TGGCGTCATG GGACTGGAAC CCTCTTCCTA CAAGACCTCT 
GTGTACCGAC AGCAGCAGCC TGCGGTGCCC CAAGGACAGC GCCTTCGCCA ACAGCTCCAG 
GCAAAGATAC AGAGTCAGGG GATGTTGGGA CAGTCATCTG TCCATCAGAT GACTCCCAGC 
TCTTCCTACG GTTTGCAGAC CTCCCAGGGC TATACTCCTT ATGTTTCTCA TGTGGGATTG 
CAGCAACACA CAGGCCCCGC AGGTACCATG GTGCCCCCCA GCTACTCCAG CCAGCCTTAT 
CAGAGCACCC ACCCTTCTAC CAATCCTACT CTTGTAGATC CTACTCGCCA CCTGCAACAG 
CGGCCCAGTG GCTATGTGCA CCAGCAGGCC CCAACCTACG GACATGGGCT GACCTCCAGT 
CAGAGGTTTT CACACCAGAC ACTGCAGCAA ACACCCATGA TGGGTACCAT GACTCCACTG 
GGCGCCCAGG GCGTCCAGGC CGGCGTCCGA TCAGCTTCCA TCTTGCCTGA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGCAGCA ATACCACATC CGGCAGCAGC AGCAGCAGCA GATCCTGCGG 
GTAAGGCCCT GGGATTTCCT CTGGGATCTG GGAGCCCAAG AAGGAGGGGA GGCACAAGCT 
CTTCCAGGTT GTTATGAAGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489442.1
CDS	170..5953
Translation	MAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGTPSPDKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633674.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633674.1

ORF Insert Sequence:

ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGCGGGG GCTGTGGTTC CACTATAGGG 
CCCTTGCCCC ATGATGTAGA AGTGGCAATC CGGCAGTGGG ACTACAATGA GAAGCTGGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTACTTGAG 
TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGTT GCCCCTGCTG 
CTTCGATACT CTGGGGAATT CGTTCAGTCT GCATACCTCT CTCGCCGCCT TGCCTACTTC 
TGTACCCGGA GGCTGGCCCT GCAGCTGGAT GGCGTGAGCA GTCACTCGTC TCATGTGATG 
TCTGCTCAGT CGACAGGCAC ACTGCCCACC ACCCCTGCTC CTCAGCCCCC GACTAGCAGC 
ACACCCTCTA CACCCTTTAG TGACCTACTT ATGTGCCCTC AGCACCGGCC CCTAGTTTTT 
GGCCTCAGCT GTATCCTTCA GACCATCCTC CTGTGTTGTC CTAGTGCCCT GGTTTGGCAC 
TACTCACTGA CTGATAGCCG AATTAAGACT GGCTCACCAC TTGACCACCT GCCTATTGCC 
CCCTCCAACC TGCCCATGCC AGAGGGCAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG 
TTGCGGGAGA TTGAGCAGCA GATCAAGGAG CGAGGACAGG CAGTTGAGGT TCGCTGGTCT 
TTTGATAAGT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTGCT CCATACTTTG 
GAAGTGCTGG ACAGCCATAG TTTTGAGCGC TCTGACTTCA GCAACTCTCT TGACTCCTTG 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT 
GATGCTGTGG TATCATTACT GTGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT 
CGTGCTATGG TGGTAGCCAA GCTGCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CAGAAGCCGC AGATGAAAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT 
CCTAGTGCTC CCATTTTCCA GGATGTTCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGACC CCCGAAGTGA GAGTGAGCGG GTGGAGTTCT TTAACTTGGT ACTGCTGTTC 
TGTGAACTCA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC CCTCATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGACCCT 
GCTGATGACC CAGAGCGCAA GGAGGCTGAG GGCAGCAGCA GTAGCAAGCT GGAGGATCCG 
GGGCTCTCGG AGTCTATGGA CATAGACCCT AGTTCCAGTG TACTGTTTGA GGACATGGAG 
AAGCCTGATT TCTCATTGTT CTCTCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC 
CCTGAGAAAC CAGATGTTGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATAGAAGGG 
ACCCTTGGGG TTCTTTATGA CCAGCCACGG CATGTGCAGT ATGCCACACA CTTTCCCATC 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGACATGCC ATCAAGAAAA TAACCAAGGA TATCCTGAAG 
GTTCTGAACC GCAAGGGGAC GGCGGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGACGGCG CAACCGGCCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCATCTTTC ACATTATGAC 
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGTTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTACT GAATGAACTG 
AGTGTAGTTG AGGCTGAGTT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACC 
AGCCTGTGCC TGTGCATTGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC 
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TAGTGAAGCA TGGGATGAAC 
CGGTCAGATG GCTCCTCTGC AGAACGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAGCTCTTCA GCGACTTCTG CTCCAAGGTG 
AAGAACACCA TCTACTGCAA CGTGGAGCCG TCAGAATCCA ATATGCGCTG GGCACCTGAG 
TTCATGATTG ACACTCTGGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG 
CTGACCGGCT ATTGCAAGTC ACTGAGTGCG GAATGGCTAG GAGTCCTTAA AGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGACCTCC TCTGCAATGT AGATGTCAGT 
GACCTGTCTT TTCATGATTC CCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT 
TTGCTCCTAG AGGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGC 
AGTGAGCAGG ATTCTGAGCC CGGGGCCCGA CTTACCTGCC GCATCCTCCT TCACCTTTTC 
AAGACACCTC AACTCAATCC TTGCCAGTCG GATGGAACTC CTTCCCCAGA CAAGCCTACT 
GTAGGAATCC GCTCCTCCTG TGACCGCCAC CTGCTGGCTG CCTCCCAGAA CCGCATCGTG 
GATGGAGCTG TGTTTGCTGT TCTCAAGGCT GTGTTTGTAC TTGGCGATGC GGAACTGAAG 
GGTTCAGGCT TCACTGTGAC AGGAGGAACA GAAGAACTTC CAGAGGAGGA GGGGGGAGGT 
GGCAGTGGCG GTCGGAGGCA GGGTGGCCGC AACATCTCTG TGGAGACAGC CAGTCTGGAT 
GTCTATGCCA AGTACGTGCT GCGCAGCATC TGCCAACAGG AATGGGTAGG AGAACGTTGC 
CTTAAATCGC TGTGTGAGGA CAGCAATGAC CTACAAGACC CAGTGTTGAG TAGCGCCCAG 
GCCCAGCGCC TCATGCAGCT CATCTGCTAC CCACATCGGC TGCTGGACAA TGAGGATGGG 
GAAAATCCCC AGCGACAACG CATTAAGCGT ATTCTCCAGA ACTTGGACCA GTGGACCATG 
CGTCAGTCTT CCCTGGAGCT GCAGCTCATG ATCAAGCAGA CCCCTAACAA TGAGATGAAC 
TCCCTCTTAG AGAACATTGC CAAGGCCACA ATCGAGGTGT TCCAACAGTC AGCAGAGACA 
GGGTCATCTT CTGGAAACAC TGCAAGCAAC ATGCCCAGCA GCAGCAAGAC CAAGCCTGTG 
CTCAGCTCTC TGGAGCGCTC TGGTGTATGG CTGGTGGCCC CCCTCATTGC TAAACTGCCC 
ACCTCAGTCC AGGGGCATGT GTTAAAGGCT GCTGGGGAGG AATTGGAGAA GGGCCAGCAC 
CTGGGTTCCT CTTCCCGCAA AGAACGTGAT CGACAAAAGC AAAAGAGTAT GTCCCTGTTG 
AGCCAGCAGC CTTTCTTGTC CCTGGTGCTG ACGTGTCTGA AAGGGCAGGA TGAGCAGCGT 
GAGGGACTCC TTACCTCCCT TTACAGCCAA GTGCACCAGA TTGTGAATAA TTGGCGAGAT 
GACCAGTACT TAGATGATTG CAAACCAAAG CAGCTAATGC ATGAGGCACT CAAACTGCGG 
CTCAACCTGG TGGGGGGCAT GTTTGACACG GTGCAGCGCA GCACCCAACA GACCACAGAG 
TGGGCTGTGC TCCTCCTGGA GATCATCATC AGCGGCACTG TCGACATGCA GTCCAACAAC 
GAGCTCTTCA CCACTGTATT GGACATGCTG AGCGTGCTCA TCAATGGGAC CCTGGCTGCG 
GACATGTCCA GCATCTCTCA AGGCAGCATG GAGGAAAACA AACGCGCCTA CATGAACCTG 
GTGAAGAAGC TGCGGAAAGA GTTGGGGGAG CGCCAATCAG ACAGTCTGGA AAAAGTTCGC 
CAGCTGCTGC CACTGCCCAA GCAGACCCGA GATGTCATCA CATGTGAGCC ACAGGGCTCC 
CTTATTGACA CCAAGGGCAA CAAGATTGCC GGCTTTGATT CCATCTTCAA GAAAGAGGGT 
CTACAGGTTT CCACCAAACA AAAGATCTCT CCCTGGGATC TTTTTGAGGG GCTGAAGCCG 
TCTGCACCAC TCTCTTGGGG CTGGTTTGGA ACAGTCCGGG TCGACAGGCG AGTGGCCCGA 
GGCGAGGAGC AGCAGCGGTT GCTGCTCTAC CACACACACC TGCGGCCCCG GCCCCGTGCC 
TATTACCTGG AGCCACTGCC ACTGCCACCA GAGGATGAGG AGCCCCCCGC TCCCACCCCG 
CTAGAGCCTG AGAAAAAGGC TCCAGAGCCC CCCAAAACTG ACAAGCCTGG GGCTGCTCCA 
CCTAGTACTG AGGAACGCAA GAAGAAGTCC ACGAAGGGCA AGAAACGCAG CCAGCCTGCT 
GCCAAGACAG AGGACTATGG AATGGGCCCA GGGCGGAGTG GCCCCTATGG TGTGACCGTG 
CCTCCGGACC TCCTGCACCA CGCCAACCCT GGTTCCATAT CCCACCTTAG CTACAGGCAG 
GGTTCCATAG GCCTGTACAC CCAGAACCAG CCACTACCTG CAGGTGGCCC TCGTGTGGAC 
CCATACCGCC CTATGCGGTT ACCAATGCAG AAGCTGCCGA CCCGACCACC TTACCCTGGA 
GTGCTACCCA CAGCCGTGAC TGGCGTCATG GGACTGGAAC CCTCTTCCTA CAAGACCTCT 
GTGTACCGAC AGCAGCAGCC TGCGGTGCCC CAAGGACAGC GCCTTCGCCA ACAGCTCCAG 
GCAAAGATAC AGAGTCAGGG GATGTTGGGA CAGTCATCTG TCCATCAGAT GACTCCCAGC 
TCTTCCTACG GTTTGCAGAC CTCCCAGGGC TATACTCCTT ATGTTTCTCA TGTGGGATTG 
CAGCAACACA CAGGCCCCGC AGGTACCATG GTGCCCCCCA GCTACTCCAG CCAGCCTTAT 
CAGAGCACCC ACCCTTCTAC CAATCCTACT CTTGTAGATC CTACTCGCCA CCTGCAACAG 
CGGCCCAGTG GCTATGTGCA CCAGCAGGCC CCAACCTACG GACATGGGCT GACCTCCAGT 
CAGAGGTTTT CACACCAGAC ACTGCAGCAA ACACCCATGA TGGGTACCAT GACTCCACTG 
GGCGCCCAGG GCGTCCAGGC CGGCGTCCGA TCAGCTTCCA TCTTGCCTGA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGCAGCA ATACCACATC CGGCAGCAGC AGCAGCAGCA GATCCTGCGG 
GTAAGGCCCT GGGATTTCCT CTGGGATCTG GGAGCCCAAG AAGGAGGGGA GGCACAAGCT 
CTTCCAGGTT GTTATGAAGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_005614273.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_005614330.1 Equus caballus mediator complex subunit 12 (MED12), mRNA.

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CloneID	OHb27102
Clone ID Related Accession (Same CDS sequence)	XM_005614273.1
Accession Version	XM_005614273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1447862400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001877043.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTGAGTG AGCTCTGGGC GATCGGGAAT CTTAGTGACT GCGGGGATGA ACTGACGGCC 
CTGAATGTAA AACAAGGCTT CAATAACCAG CCTGCTGTCT CTGGGGATGA ACATGGCAGT 
GCCAAGAACG TCAACTTCAA TCCTGCCAAG ATCAGTTCCA ACTTCAGCAG CATTATTGCA 
GAGAAGTTAC GTTGTAACAC CCTCCCTGAC ACTGGTCGCA GGAAGCCCCA AGTGAACCAG 
AAGGACAACT TCTGGCTGGT GACGGCGCGA TCCCAGAGTG CCATTAACAC CTGGTTCACT 
GACCTGGCTG GCACCAAGCC ACTCACACAA CTAGCCAAAA AGGTCCCCAT TTTCAGTAAG 
AAGGAAGAAG TGTTTGGGTA CTTAGCCAAA TACACAGTGC CTGTGATGCG GGCTGCCTGG 
CTCATTAAGA TGACCTGTGC CTACTATGCA GCAATCACTG AGACCAAGGT TAAGAAGAGA 
CATGTCATTG ACCCCTTCAT GGAATGGACT CAGATCATCA CCAAGTACTT ATGGGAGCAG 
CTGCAAAAGA TGGCTGAATA CTACCGGCCA GGGCCTGCAG GAAGCGGGGG CTGTGGTTCC 
ACTATAGGGC CCTTGCCCCA TGATGTAGAA GTGGCAATCC GGCAGTGGGA CTACAATGAG 
AAGCTGGCCA TGTTCATGTT TCAGGATGGA ATGCTGGACA GACATGAGTT CCTGACCTGG 
GTACTTGAGT GTTTTGAGAA AATCCGCCCT GGAGAGGATG AATTGCTTAA ACTGCTGTTG 
CCCCTGCTGC TTCGATACTC TGGGGAATTC GTTCAGTCTG CATACCTCTC TCGCCGCCTT 
GCCTACTTCT GTACCCGGAG GCTGGCCCTG CAGCTGGATG GCGTGAGCAG TCACTCGTCT 
CATGTGATGT CTGCTCAGTC GACAGGCACA CTGCCCACCA CCCCTGCTCC TCAGCCCCCG 
ACTAGCAGCA CACCCTCTAC ACCCTTTAGT GACCTACTTA TGTGCCCTCA GCACCGGCCC 
CTAGTTTTTG GCCTCAGCTG TATCCTTCAG ACCATCCTCC TGTGTTGTCC TAGTGCCCTG 
GTTTGGCACT ACTCACTGAC TGATAGCCGA ATTAAGACTG GCTCACCACT TGACCACCTG 
CCTATTGCCC CCTCCAACCT GCCCATGCCA GAGGGCAACA GTGCCTTCAC TCAGCAGGTC 
CGTGCAAAGT TGCGGGAGAT TGAGCAGCAG ATCAAGGAGC GAGGACAGGC AGTTGAGGTT 
CGCTGGTCTT TTGATAAGTG CCAGGAAGCT ACTGCAGGCT TCACCATTGG ACGGGTGCTC 
CATACTTTGG AAGTGCTGGA CAGCCATAGT TTTGAGCGCT CTGACTTCAG CAACTCTCTT 
GACTCCTTGT GTAACCGAAT CTTTGGATTG GGGCCTAGCA AGGATGGGCA TGAGATCTCC 
TCAGATGATG ATGCTGTGGT ATCATTACTG TGTGAATGGG CTGTCAGCTG CAAGCGTTCT 
GGTCGGCATC GTGCTATGGT GGTAGCCAAG CTGCTGGAGA AGAGACAGGC AGAGATTGAG 
GCTGAGCGTT GTGGAGAATC AGAAGCCGCA GATGAAAAGG GTTCCATCGC CTCTGGCTCC 
CTTTCTGCTC CTAGTGCTCC CATTTTCCAG GATGTTCTCC TGCAGTTTCT GGATACACAG 
GCTCCCATGC TGACGGACCC CCGAAGTGAG AGTGAGCGGG TGGAGTTCTT TAACTTGGTA 
CTGCTGTTCT GTGAACTCAT TCGACATGAT GTTTTCTCCC ACAACATGTA TACTTGCACC 
CTCATCTCCC GAGGGGACCT TGCCTTTGGA GCCCCTGGTC CCCGGCCTCC CTCTCCCTTT 
GATGACCCTG CTGATGACCC AGAGCGCAAG GAGGCTGAGG GCAGCAGCAG TAGCAAGCTG 
GAGGATCCGG GGCTCTCGGA GTCTATGGAC ATAGACCCTA GTTCCAGTGT ACTGTTTGAG 
GACATGGAGA AGCCTGATTT CTCATTGTTC TCTCCTACTA TGCCCTGTGA GGGGAAGGGC 
AGTCCATCCC CTGAGAAACC AGATGTTGAG AAGGAGGTGA AGCCCCCACC CAAGGAGAAG 
ATAGAAGGGA CCCTTGGGGT TCTTTATGAC CAGCCACGGC ATGTGCAGTA TGCCACACAC 
TTTCCCATCC CCCAGGAGGA GTCATGCAGC CATGAGTGCA ACCAGCGGTT GGTCGTACTG 
TTTGGGGTGG GAAAGCAGCG AGATGATGCC CGACATGCCA TCAAGAAAAT AACCAAGGAT 
ATCCTGAAGG TTCTGAACCG CAAGGGGACG GCGGAAACTG ACCAGCTTGC TCCTATTGTG 
CCTCTGAATC CTGGAGACCT GACATTCTTA GGTGGGGAGG ATGGGCAGAA GCGACGGCGC 
AACCGGCCTG AAGCCTTCCC CACTGCTGAA GATATCTTTG CTAAGTTCCA GCATCTTTCA 
CATTATGACC AACACCAGGT CACGGCTCAG GTCTCCCGGA ATGTTCTGGA GCAGATCACG 
AGTTTTGCCC TTGGCATGTC ATACCACTTG CCTCTGGTGC AGCATGTGCA GTTCATCTTC 
GACCTCATGG AATATTCACT CAGCATCAGT GGCCTCATCG ACTTTGCCAT TCAGCTACTG 
AATGAACTGA GTGTAGTTGA GGCTGAGTTG CTTCTCAAAT CCTCGGATCT GGTGGGCAGC 
TACACTACCA GCCTGTGCCT GTGCATTGTG GCTGTCCTGC GGCACTATCA TGCCTGCCTC 
ATCCTCAACC AGGACCAGAT GGCACAGGTC TTTGAGGGGC TGTGTGGCGT AGTGAAGCAT 
GGGATGAACC GGTCAGATGG CTCCTCTGCA GAACGCTGTA TCCTTGCTTA TCTCTATGAT 
CTGTACACCT CCTGTAGCCA TTTAAAGAGC AAATTTGGGG AGCTCTTCAG CGACTTCTGC 
TCCAAGGTGA AGAACACCAT CTACTGCAAC GTGGAGCCGT CAGAATCCAA TATGCGCTGG 
GCACCTGAGT TCATGATTGA CACTCTGGAG AACCCTGCAG CTCACACCTT CACCTACACG 
GGGCTAGGCA AGAGTCTTAG TGAGAACCCT GCTAACCGCT ACAGCTTTGT CTGCAATGCC 
CTTATGCACG TCTGTGTGGG GCACCATGAT CCCGATAGGG TGAATGACAT CGCAATCCTG 
TGTGCAGAGC TGACCGGCTA TTGCAAGTCA CTGAGTGCGG AATGGCTAGG AGTCCTTAAA 
GCCTTGTGCT GCTCCTCTAA CAATGGCACT TGTGGTTTCA ACGACCTCCT CTGCAATGTA 
GATGTCAGTG ACCTGTCTTT TCATGATTCC CTGGCTACTT TTGTTGCCAT CCTCATCGCT 
CGGCAGTGTT TGCTCCTAGA GGATCTGATT CGCTGTGCTG CCATCCCTTC ACTCCTTAAT 
GCTGCTTGCA GTGAGCAGGA TTCTGAGCCC GGGGCCCGAC TTACCTGCCG CATCCTCCTT 
CACCTTTTCA AGACACCTCA ACTCAATCCT TGCCAGTCGG ATGGAAACAA GCCTACTGTA 
GGAATCCGCT CCTCCTGTGA CCGCCACCTG CTGGCTGCCT CCCAGAACCG CATCGTGGAT 
GGAGCTGTGT TTGCTGTTCT CAAGGCTGTG TTTGTACTTG GCGATGCGGA ACTGAAGGGT 
TCAGGCTTCA CTGTGACAGG AGGAACAGAA GAACTTCCAG AGGAGGAGGG GGGAGGTGGC 
AGTGGCGGTC GGAGGCAGGG TGGCCGCAAC ATCTCTGTGG AGACAGCCAG TCTGGATGTC 
TATGCCAAGT ACGTGCTGCG CAGCATCTGC CAACAGGAAT GGGTAGGAGA ACGTTGCCTT 
AAATCGCTGT GTGAGGACAG CAATGACCTA CAAGACCCAG TGTTGAGTAG CGCCCAGGCC 
CAGCGCCTCA TGCAGCTCAT CTGCTACCCA CATCGGCTGC TGGACAATGA GGATGGGGAA 
AATCCCCAGC GACAACGCAT TAAGCGTATT CTCCAGAACT TGGACCAGTG GACCATGCGT 
CAGTCTTCCC TGGAGCTGCA GCTCATGATC AAGCAGACCC CTAACAATGA GATGAACTCC 
CTCTTAGAGA ACATTGCCAA GGCCACAATC GAGGTGTTCC AACAGTCAGC AGAGACAGGG 
TCATCTTCTG GAAACACTGC AAGCAACATG CCCAGCAGCA GCAAGACCAA GCCTGTGCTC 
AGCTCTCTGG AGCGCTCTGG TGTATGGCTG GTGGCCCCCC TCATTGCTAA ACTGCCCACC 
TCAGTCCAGG GGCATGTGTT AAAGGCTGCT GGGGAGGAAT TGGAGAAGGG CCAGCACCTG 
GGTTCCTCTT CCCGCAAAGA ACGTGATCGA CAAAAGCAAA AGAGTATGTC CCTGTTGAGC 
CAGCAGCCTT TCTTGTCCCT GGTGCTGACG TGTCTGAAAG GGCAGGATGA GCAGCGTGAG 
GGACTCCTTA CCTCCCTTTA CAGCCAAGTG CACCAGATTG TGAATAATTG GCGAGATGAC 
CAGTACTTAG ATGATTGCAA ACCAAAGCAG CTAATGCATG AGGCACTCAA ACTGCGGCTC 
AACCTGGTGG GGGGCATGTT TGACACGGTG CAGCGCAGCA CCCAACAGAC CACAGAGTGG 
GCTGTGCTCC TCCTGGAGAT CATCATCAGC GGCACTGTCG ACATGCAGTC CAACAACGAG 
CTCTTCACCA CTGTATTGGA CATGCTGAGC GTGCTCATCA ATGGGACCCT GGCTGCGGAC 
ATGTCCAGCA TCTCTCAAGG CAGCATGGAG GAAAACAAAC GCGCCTACAT GAACCTGGTG 
AAGAAGCTGC GGAAAGAGTT GGGGGAGCGC CAATCAGACA GTCTGGAAAA AGTTCGCCAG 
CTGCTGCCAC TGCCCAAGCA GACCCGAGAT GTCATCACAT GTGAGCCACA GGGCTCCCTT 
ATTGACACCA AGGGCAACAA GATTGCCGGC TTTGATTCCA TCTTCAAGAA AGAGGGTCTA 
CAGGTTTCCA CCAAACAAAA GATCTCTCCC TGGGATCTTT TTGAGGGGCT GAAGCCGTCT 
GCACCACTCT CTTGGGGCTG GTTTGGAACA GTCCGGGTCG ACAGGCGAGT GGCCCGAGGC 
GAGGAGCAGC AGCGGTTGCT GCTCTACCAC ACACACCTGC GGCCCCGGCC CCGTGCCTAT 
TACCTGGAGC CACTGCCACT GCCACCAGAG GATGAGGAGC CCCCCGCTCC CACCCCGCTA 
GAGCCTGAGA AAAAGGCTCC AGAGCCCCCC AAAACTGACA AGCCTGGGGC TGCTCCACCT 
AGTACTGAGG AACGCAAGAA GAAGTCCACG AAGGGCAAGA AACGCAGCCA GCCTGCTGCC 
AAGACAGAGG ACTATGGAAT GGGCCCAGGG CGGAGTGGCC CCTATGGTGT GACCGTGCCT 
CCGGACCTCC TGCACCACGC CAACCCTGGT TCCATATCCC ACCTTAGCTA CAGGCAGGGT 
TCCATAGGCC TGTACACCCA GAACCAGCCA CTACCTGCAG GTGGCCCTCG TGTGGACCCA 
TACCGCCCTA TGCGGTTACC AATGCAGAAG CTGCCGACCC GACCACCTTA CCCTGGAGTG 
CTACCCACAG CCGTGACTGG CGTCATGGGA CTGGAACCCT CTTCCTACAA GACCTCTGTG 
TACCGACAGC AGCAGCCTGC GGTGCCCCAA GGACAGCGCC TTCGCCAACA GCTCCAGGCA 
AAGATACAGA GTCAGGGGAT GTTGGGACAG TCATCTGTCC ATCAGATGAC TCCCAGCTCT 
TCCTACGGTT TGCAGACCTC CCAGGGCTAT ACTCCTTATG TTTCTCATGT GGGATTGCAG 
CAACACACAG GCCCCGCAGA TCCTACTCGC CACCTGCAAC AGCGGCCCAG TGGCTATGTG 
CACCAGCAGG CCCCAACCTA CGGACATGGG CTGACCTCCA GTCAGAGGTT TTCACACCAG 
ACACTGCAGC AAACACCCAT GATGGGTACC ATGACTCCAC TGGGCGCCCA GGGCGTCCAG 
GCCGGCGTCC GATCAGCTTC CATCTTGCCT GAGCAGCAGC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGCAG 
CAATACCACA TCCGGCAGCA GCAGCAGCAG CAGATCCTGC GGCAGCAGCA GCAACAGCAG 
CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAGCAACAGC AACAGCAGCA GCAGCAACAG 
CAAGCACACC AGCAGCAGCA GCAGCAGGCG GCTCCTCCCC AACCCCAGCC CCAGTCCCAG 
CCCCAGTTCC AGCGCCAGGG GCTTCAGCAG ACACAGCAAC AGCAACAGAC AGCAGCTTTG 
GTCCGGCAGC TCCAACAACA GCTCTCCAAT ACCCAGCCAC AGCCCAGTAC CAACCTATTT 
GGACGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005614330.1
CDS	1..6432
Translation	MLSELWAIGNLSDCGDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPADPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQQAHQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNLFGRF

Target ORF information:

RefSeq Version	XM_005614273.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005614273.1

ORF Insert Sequence:

ATGTTGAGTG AGCTCTGGGC GATCGGGAAT CTTAGTGACT GCGGGGATGA ACTGACGGCC 
CTGAATGTAA AACAAGGCTT CAATAACCAG CCTGCTGTCT CTGGGGATGA ACATGGCAGT 
GCCAAGAACG TCAACTTCAA TCCTGCCAAG ATCAGTTCCA ACTTCAGCAG CATTATTGCA 
GAGAAGTTAC GTTGTAACAC CCTCCCTGAC ACTGGTCGCA GGAAGCCCCA AGTGAACCAG 
AAGGACAACT TCTGGCTGGT GACGGCGCGA TCCCAGAGTG CCATTAACAC CTGGTTCACT 
GACCTGGCTG GCACCAAGCC ACTCACACAA CTAGCCAAAA AGGTCCCCAT TTTCAGTAAG 
AAGGAAGAAG TGTTTGGGTA CTTAGCCAAA TACACAGTGC CTGTGATGCG GGCTGCCTGG 
CTCATTAAGA TGACCTGTGC CTACTATGCA GCAATCACTG AGACCAAGGT TAAGAAGAGA 
CATGTCATTG ACCCCTTCAT GGAATGGACT CAGATCATCA CCAAGTACTT ATGGGAGCAG 
CTGCAAAAGA TGGCTGAATA CTACCGGCCA GGGCCTGCAG GAAGCGGGGG CTGTGGTTCC 
ACTATAGGGC CCTTGCCCCA TGATGTAGAA GTGGCAATCC GGCAGTGGGA CTACAATGAG 
AAGCTGGCCA TGTTCATGTT TCAGGATGGA ATGCTGGACA GACATGAGTT CCTGACCTGG 
GTACTTGAGT GTTTTGAGAA AATCCGCCCT GGAGAGGATG AATTGCTTAA ACTGCTGTTG 
CCCCTGCTGC TTCGATACTC TGGGGAATTC GTTCAGTCTG CATACCTCTC TCGCCGCCTT 
GCCTACTTCT GTACCCGGAG GCTGGCCCTG CAGCTGGATG GCGTGAGCAG TCACTCGTCT 
CATGTGATGT CTGCTCAGTC GACAGGCACA CTGCCCACCA CCCCTGCTCC TCAGCCCCCG 
ACTAGCAGCA CACCCTCTAC ACCCTTTAGT GACCTACTTA TGTGCCCTCA GCACCGGCCC 
CTAGTTTTTG GCCTCAGCTG TATCCTTCAG ACCATCCTCC TGTGTTGTCC TAGTGCCCTG 
GTTTGGCACT ACTCACTGAC TGATAGCCGA ATTAAGACTG GCTCACCACT TGACCACCTG 
CCTATTGCCC CCTCCAACCT GCCCATGCCA GAGGGCAACA GTGCCTTCAC TCAGCAGGTC 
CGTGCAAAGT TGCGGGAGAT TGAGCAGCAG ATCAAGGAGC GAGGACAGGC AGTTGAGGTT 
CGCTGGTCTT TTGATAAGTG CCAGGAAGCT ACTGCAGGCT TCACCATTGG ACGGGTGCTC 
CATACTTTGG AAGTGCTGGA CAGCCATAGT TTTGAGCGCT CTGACTTCAG CAACTCTCTT 
GACTCCTTGT GTAACCGAAT CTTTGGATTG GGGCCTAGCA AGGATGGGCA TGAGATCTCC 
TCAGATGATG ATGCTGTGGT ATCATTACTG TGTGAATGGG CTGTCAGCTG CAAGCGTTCT 
GGTCGGCATC GTGCTATGGT GGTAGCCAAG CTGCTGGAGA AGAGACAGGC AGAGATTGAG 
GCTGAGCGTT GTGGAGAATC AGAAGCCGCA GATGAAAAGG GTTCCATCGC CTCTGGCTCC 
CTTTCTGCTC CTAGTGCTCC CATTTTCCAG GATGTTCTCC TGCAGTTTCT GGATACACAG 
GCTCCCATGC TGACGGACCC CCGAAGTGAG AGTGAGCGGG TGGAGTTCTT TAACTTGGTA 
CTGCTGTTCT GTGAACTCAT TCGACATGAT GTTTTCTCCC ACAACATGTA TACTTGCACC 
CTCATCTCCC GAGGGGACCT TGCCTTTGGA GCCCCTGGTC CCCGGCCTCC CTCTCCCTTT 
GATGACCCTG CTGATGACCC AGAGCGCAAG GAGGCTGAGG GCAGCAGCAG TAGCAAGCTG 
GAGGATCCGG GGCTCTCGGA GTCTATGGAC ATAGACCCTA GTTCCAGTGT ACTGTTTGAG 
GACATGGAGA AGCCTGATTT CTCATTGTTC TCTCCTACTA TGCCCTGTGA GGGGAAGGGC 
AGTCCATCCC CTGAGAAACC AGATGTTGAG AAGGAGGTGA AGCCCCCACC CAAGGAGAAG 
ATAGAAGGGA CCCTTGGGGT TCTTTATGAC CAGCCACGGC ATGTGCAGTA TGCCACACAC 
TTTCCCATCC CCCAGGAGGA GTCATGCAGC CATGAGTGCA ACCAGCGGTT GGTCGTACTG 
TTTGGGGTGG GAAAGCAGCG AGATGATGCC CGACATGCCA TCAAGAAAAT AACCAAGGAT 
ATCCTGAAGG TTCTGAACCG CAAGGGGACG GCGGAAACTG ACCAGCTTGC TCCTATTGTG 
CCTCTGAATC CTGGAGACCT GACATTCTTA GGTGGGGAGG ATGGGCAGAA GCGACGGCGC 
AACCGGCCTG AAGCCTTCCC CACTGCTGAA GATATCTTTG CTAAGTTCCA GCATCTTTCA 
CATTATGACC AACACCAGGT CACGGCTCAG GTCTCCCGGA ATGTTCTGGA GCAGATCACG 
AGTTTTGCCC TTGGCATGTC ATACCACTTG CCTCTGGTGC AGCATGTGCA GTTCATCTTC 
GACCTCATGG AATATTCACT CAGCATCAGT GGCCTCATCG ACTTTGCCAT TCAGCTACTG 
AATGAACTGA GTGTAGTTGA GGCTGAGTTG CTTCTCAAAT CCTCGGATCT GGTGGGCAGC 
TACACTACCA GCCTGTGCCT GTGCATTGTG GCTGTCCTGC GGCACTATCA TGCCTGCCTC 
ATCCTCAACC AGGACCAGAT GGCACAGGTC TTTGAGGGGC TGTGTGGCGT AGTGAAGCAT 
GGGATGAACC GGTCAGATGG CTCCTCTGCA GAACGCTGTA TCCTTGCTTA TCTCTATGAT 
CTGTACACCT CCTGTAGCCA TTTAAAGAGC AAATTTGGGG AGCTCTTCAG CGACTTCTGC 
TCCAAGGTGA AGAACACCAT CTACTGCAAC GTGGAGCCGT CAGAATCCAA TATGCGCTGG 
GCACCTGAGT TCATGATTGA CACTCTGGAG AACCCTGCAG CTCACACCTT CACCTACACG 
GGGCTAGGCA AGAGTCTTAG TGAGAACCCT GCTAACCGCT ACAGCTTTGT CTGCAATGCC 
CTTATGCACG TCTGTGTGGG GCACCATGAT CCCGATAGGG TGAATGACAT CGCAATCCTG 
TGTGCAGAGC TGACCGGCTA TTGCAAGTCA CTGAGTGCGG AATGGCTAGG AGTCCTTAAA 
GCCTTGTGCT GCTCCTCTAA CAATGGCACT TGTGGTTTCA ACGACCTCCT CTGCAATGTA 
GATGTCAGTG ACCTGTCTTT TCATGATTCC CTGGCTACTT TTGTTGCCAT CCTCATCGCT 
CGGCAGTGTT TGCTCCTAGA GGATCTGATT CGCTGTGCTG CCATCCCTTC ACTCCTTAAT 
GCTGCTTGCA GTGAGCAGGA TTCTGAGCCC GGGGCCCGAC TTACCTGCCG CATCCTCCTT 
CACCTTTTCA AGACACCTCA ACTCAATCCT TGCCAGTCGG ATGGAAACAA GCCTACTGTA 
GGAATCCGCT CCTCCTGTGA CCGCCACCTG CTGGCTGCCT CCCAGAACCG CATCGTGGAT 
GGAGCTGTGT TTGCTGTTCT CAAGGCTGTG TTTGTACTTG GCGATGCGGA ACTGAAGGGT 
TCAGGCTTCA CTGTGACAGG AGGAACAGAA GAACTTCCAG AGGAGGAGGG GGGAGGTGGC 
AGTGGCGGTC GGAGGCAGGG TGGCCGCAAC ATCTCTGTGG AGACAGCCAG TCTGGATGTC 
TATGCCAAGT ACGTGCTGCG CAGCATCTGC CAACAGGAAT GGGTAGGAGA ACGTTGCCTT 
AAATCGCTGT GTGAGGACAG CAATGACCTA CAAGACCCAG TGTTGAGTAG CGCCCAGGCC 
CAGCGCCTCA TGCAGCTCAT CTGCTACCCA CATCGGCTGC TGGACAATGA GGATGGGGAA 
AATCCCCAGC GACAACGCAT TAAGCGTATT CTCCAGAACT TGGACCAGTG GACCATGCGT 
CAGTCTTCCC TGGAGCTGCA GCTCATGATC AAGCAGACCC CTAACAATGA GATGAACTCC 
CTCTTAGAGA ACATTGCCAA GGCCACAATC GAGGTGTTCC AACAGTCAGC AGAGACAGGG 
TCATCTTCTG GAAACACTGC AAGCAACATG CCCAGCAGCA GCAAGACCAA GCCTGTGCTC 
AGCTCTCTGG AGCGCTCTGG TGTATGGCTG GTGGCCCCCC TCATTGCTAA ACTGCCCACC 
TCAGTCCAGG GGCATGTGTT AAAGGCTGCT GGGGAGGAAT TGGAGAAGGG CCAGCACCTG 
GGTTCCTCTT CCCGCAAAGA ACGTGATCGA CAAAAGCAAA AGAGTATGTC CCTGTTGAGC 
CAGCAGCCTT TCTTGTCCCT GGTGCTGACG TGTCTGAAAG GGCAGGATGA GCAGCGTGAG 
GGACTCCTTA CCTCCCTTTA CAGCCAAGTG CACCAGATTG TGAATAATTG GCGAGATGAC 
CAGTACTTAG ATGATTGCAA ACCAAAGCAG CTAATGCATG AGGCACTCAA ACTGCGGCTC 
AACCTGGTGG GGGGCATGTT TGACACGGTG CAGCGCAGCA CCCAACAGAC CACAGAGTGG 
GCTGTGCTCC TCCTGGAGAT CATCATCAGC GGCACTGTCG ACATGCAGTC CAACAACGAG 
CTCTTCACCA CTGTATTGGA CATGCTGAGC GTGCTCATCA ATGGGACCCT GGCTGCGGAC 
ATGTCCAGCA TCTCTCAAGG CAGCATGGAG GAAAACAAAC GCGCCTACAT GAACCTGGTG 
AAGAAGCTGC GGAAAGAGTT GGGGGAGCGC CAATCAGACA GTCTGGAAAA AGTTCGCCAG 
CTGCTGCCAC TGCCCAAGCA GACCCGAGAT GTCATCACAT GTGAGCCACA GGGCTCCCTT 
ATTGACACCA AGGGCAACAA GATTGCCGGC TTTGATTCCA TCTTCAAGAA AGAGGGTCTA 
CAGGTTTCCA CCAAACAAAA GATCTCTCCC TGGGATCTTT TTGAGGGGCT GAAGCCGTCT 
GCACCACTCT CTTGGGGCTG GTTTGGAACA GTCCGGGTCG ACAGGCGAGT GGCCCGAGGC 
GAGGAGCAGC AGCGGTTGCT GCTCTACCAC ACACACCTGC GGCCCCGGCC CCGTGCCTAT 
TACCTGGAGC CACTGCCACT GCCACCAGAG GATGAGGAGC CCCCCGCTCC CACCCCGCTA 
GAGCCTGAGA AAAAGGCTCC AGAGCCCCCC AAAACTGACA AGCCTGGGGC TGCTCCACCT 
AGTACTGAGG AACGCAAGAA GAAGTCCACG AAGGGCAAGA AACGCAGCCA GCCTGCTGCC 
AAGACAGAGG ACTATGGAAT GGGCCCAGGG CGGAGTGGCC CCTATGGTGT GACCGTGCCT 
CCGGACCTCC TGCACCACGC CAACCCTGGT TCCATATCCC ACCTTAGCTA CAGGCAGGGT 
TCCATAGGCC TGTACACCCA GAACCAGCCA CTACCTGCAG GTGGCCCTCG TGTGGACCCA 
TACCGCCCTA TGCGGTTACC AATGCAGAAG CTGCCGACCC GACCACCTTA CCCTGGAGTG 
CTACCCACAG CCGTGACTGG CGTCATGGGA CTGGAACCCT CTTCCTACAA GACCTCTGTG 
TACCGACAGC AGCAGCCTGC GGTGCCCCAA GGACAGCGCC TTCGCCAACA GCTCCAGGCA 
AAGATACAGA GTCAGGGGAT GTTGGGACAG TCATCTGTCC ATCAGATGAC TCCCAGCTCT 
TCCTACGGTT TGCAGACCTC CCAGGGCTAT ACTCCTTATG TTTCTCATGT GGGATTGCAG 
CAACACACAG GCCCCGCAGA TCCTACTCGC CACCTGCAAC AGCGGCCCAG TGGCTATGTG 
CACCAGCAGG CCCCAACCTA CGGACATGGG CTGACCTCCA GTCAGAGGTT TTCACACCAG 
ACACTGCAGC AAACACCCAT GATGGGTACC ATGACTCCAC TGGGCGCCCA GGGCGTCCAG 
GCCGGCGTCC GATCAGCTTC CATCTTGCCT GAGCAGCAGC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGCAG 
CAATACCACA TCCGGCAGCA GCAGCAGCAG CAGATCCTGC GGCAGCAGCA GCAACAGCAG 
CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAGCAACAGC AACAGCAGCA GCAGCAACAG 
CAAGCACACC AGCAGCAGCA GCAGCAGGCG GCTCCTCCCC AACCCCAGCC CCAGTCCCAG 
CCCCAGTTCC AGCGCCAGGG GCTTCAGCAG ACACAGCAAC AGCAACAGAC AGCAGCTTTG 
GTCCGGCAGC TCCAACAACA GCTCTCCAAT ACCCAGCCAC AGCCCAGTAC CAACCTATTT 
GGACGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633665.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489433.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

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CloneID	OHb71697
Clone ID Related Accession (Same CDS sequence)	XM_023633665.1
Accession Version	XM_023633665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGGCAAAGA TACAGAGTCA GGGGATGTTG GGACAGTCAT CTGTCCATCA GATGACTCCC 
AGCTCTTCCT ACGGTTTGCA GACCTCCCAG GGCTATACTC CTTATGTTTC TCATGTGGGA 
TTGCAGCAAC ACACAGGCCC CGCAGGTACC ATGGTGCCCC CCAGCTACTC CAGCCAGCCT 
TATCAGAGCA CCCACCCTTC TACCAATCCT ACTCTTGTAG ATCCTACTCG CCACCTGCAA 
CAGCGGCCCA GTGGCTATGT GCACCAGCAG GCCCCAACCT ACGGACATGG GCTGACCTCC 
AGTCAGAGGT TTTCACACCA GACACTGCAG CAAACACCCA TGATGGGTAC CATGACTCCA 
CTGGGCGCCC AGGGCGTCCA GGCCGGCGTC CGATCAGCTT CCATCTTGCC TGAGCAGCAG 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAACAGCA GCAATACCAC ATCCGGCAGC AGCAGCAGCA GCAGATCCTG 
CGGGTAAGGC CCTGGGATTT CCTCTGGGAT CTGGGAGCCC AAGAAGGAGG GGAGGCACAA 
GCTCTTCCAG GTTGTTATGA AGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489433.1
CDS	55..6441
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGTPSPDKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633665.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633665.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGGCAAAGA TACAGAGTCA GGGGATGTTG GGACAGTCAT CTGTCCATCA GATGACTCCC 
AGCTCTTCCT ACGGTTTGCA GACCTCCCAG GGCTATACTC CTTATGTTTC TCATGTGGGA 
TTGCAGCAAC ACACAGGCCC CGCAGGTACC ATGGTGCCCC CCAGCTACTC CAGCCAGCCT 
TATCAGAGCA CCCACCCTTC TACCAATCCT ACTCTTGTAG ATCCTACTCG CCACCTGCAA 
CAGCGGCCCA GTGGCTATGT GCACCAGCAG GCCCCAACCT ACGGACATGG GCTGACCTCC 
AGTCAGAGGT TTTCACACCA GACACTGCAG CAAACACCCA TGATGGGTAC CATGACTCCA 
CTGGGCGCCC AGGGCGTCCA GGCCGGCGTC CGATCAGCTT CCATCTTGCC TGAGCAGCAG 
CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAACAGCA GCAATACCAC ATCCGGCAGC AGCAGCAGCA GCAGATCCTG 
CGGGTAAGGC CCTGGGATTT CCTCTGGGAT CTGGGAGCCC AAGAAGGAGG GGAGGCACAA 
GCTCTTCCAG GTTGTTATGA AGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633666.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489434.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

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CloneID	OHb71698
Clone ID Related Accession (Same CDS sequence)	XM_023633666.1
Accession Version	XM_023633666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGGCAAAGA TAAGTCAGGG GATGTTGGGA CAGTCATCTG TCCATCAGAT GACTCCCAGC 
TCTTCCTACG GTTTGCAGAC CTCCCAGGGC TATACTCCTT ATGTTTCTCA TGTGGGATTG 
CAGCAACACA CAGGCCCCGC AGGTACCATG GTGCCCCCCA GCTACTCCAG CCAGCCTTAT 
CAGAGCACCC ACCCTTCTAC CAATCCTACT CTTGTAGATC CTACTCGCCA CCTGCAACAG 
CGGCCCAGTG GCTATGTGCA CCAGCAGGCC CCAACCTACG GACATGGGCT GACCTCCAGT 
CAGAGGTTTT CACACCAGAC ACTGCAGCAA ACACCCATGA TGGGTACCAT GACTCCACTG 
GGCGCCCAGG GCGTCCAGGC CGGCGTCCGA TCAGCTTCCA TCTTGCCTGA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGCAGCA ATACCACATC CGGCAGCAGC AGCAGCAGCA GATCCTGCGG 
GTAAGGCCCT GGGATTTCCT CTGGGATCTG GGAGCCCAAG AAGGAGGGGA GGCACAAGCT 
CTTCCAGGTT GTTATGAAGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489434.1
CDS	56..6439
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGTPSPDKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKISQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633666.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633666.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGGCAAAGA TAAGTCAGGG GATGTTGGGA CAGTCATCTG TCCATCAGAT GACTCCCAGC 
TCTTCCTACG GTTTGCAGAC CTCCCAGGGC TATACTCCTT ATGTTTCTCA TGTGGGATTG 
CAGCAACACA CAGGCCCCGC AGGTACCATG GTGCCCCCCA GCTACTCCAG CCAGCCTTAT 
CAGAGCACCC ACCCTTCTAC CAATCCTACT CTTGTAGATC CTACTCGCCA CCTGCAACAG 
CGGCCCAGTG GCTATGTGCA CCAGCAGGCC CCAACCTACG GACATGGGCT GACCTCCAGT 
CAGAGGTTTT CACACCAGAC ACTGCAGCAA ACACCCATGA TGGGTACCAT GACTCCACTG 
GGCGCCCAGG GCGTCCAGGC CGGCGTCCGA TCAGCTTCCA TCTTGCCTGA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGCAGCA ATACCACATC CGGCAGCAGC AGCAGCAGCA GATCCTGCGG 
GTAAGGCCCT GGGATTTCCT CTGGGATCTG GGAGCCCAAG AAGGAGGGGA GGCACAAGCT 
CTTCCAGGTT GTTATGAAGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633667.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489435.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

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CloneID	OHb71699
Clone ID Related Accession (Same CDS sequence)	XM_023633667.1
Accession Version	XM_023633667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGCAGAGTC AGGGGATGTT GGGACAGTCA TCTGTCCATC AGATGACTCC CAGCTCTTCC 
TACGGTTTGC AGACCTCCCA GGGCTATACT CCTTATGTTT CTCATGTGGG ATTGCAGCAA 
CACACAGGCC CCGCAGGTAC CATGGTGCCC CCCAGCTACT CCAGCCAGCC TTATCAGAGC 
ACCCACCCTT CTACCAATCC TACTCTTGTA GATCCTACTC GCCACCTGCA ACAGCGGCCC 
AGTGGCTATG TGCACCAGCA GGCCCCAACC TACGGACATG GGCTGACCTC CAGTCAGAGG 
TTTTCACACC AGACACTGCA GCAAACACCC ATGATGGGTA CCATGACTCC ACTGGGCGCC 
CAGGGCGTCC AGGCCGGCGT CCGATCAGCT TCCATCTTGC CTGAGCAGCA GCAACAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAACAGC AGCAATACCA CATCCGGCAG CAGCAGCAGC AGCAGATCCT GCGGGTAAGG 
CCCTGGGATT TCCTCTGGGA TCTGGGAGCC CAAGAAGGAG GGGAGGCACA AGCTCTTCCA 
GGTTGTTATG AAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489435.1
CDS	55..6432
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGTPSPDKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633667.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633667.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGCAGAGTC AGGGGATGTT GGGACAGTCA TCTGTCCATC AGATGACTCC CAGCTCTTCC 
TACGGTTTGC AGACCTCCCA GGGCTATACT CCTTATGTTT CTCATGTGGG ATTGCAGCAA 
CACACAGGCC CCGCAGGTAC CATGGTGCCC CCCAGCTACT CCAGCCAGCC TTATCAGAGC 
ACCCACCCTT CTACCAATCC TACTCTTGTA GATCCTACTC GCCACCTGCA ACAGCGGCCC 
AGTGGCTATG TGCACCAGCA GGCCCCAACC TACGGACATG GGCTGACCTC CAGTCAGAGG 
TTTTCACACC AGACACTGCA GCAAACACCC ATGATGGGTA CCATGACTCC ACTGGGCGCC 
CAGGGCGTCC AGGCCGGCGT CCGATCAGCT TCCATCTTGC CTGAGCAGCA GCAACAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAACAGC AGCAATACCA CATCCGGCAG CAGCAGCAGC AGCAGATCCT GCGGGTAAGG 
CCCTGGGATT TCCTCTGGGA TCTGGGAGCC CAAGAAGGAG GGGAGGCACA AGCTCTTCCA 
GGTTGTTATG AAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633668.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489436.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X4, mRNA.

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CloneID	OHb71700
Clone ID Related Accession (Same CDS sequence)	XM_023633668.1
Accession Version	XM_023633668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA ACAAGCCTAC TGTAGGAATC 
CGCTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGAGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTTGGCGATG CGGAACTGAA GGGTTCAGGC 
TTCACTGTGA CAGGAGGAAC AGAAGAACTT CCAGAGGAGG AGGGGGGAGG TGGCAGTGGC 
GGTCGGAGGC AGGGTGGCCG CAACATCTCT GTGGAGACAG CCAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGCAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAATCG 
CTGTGTGAGG ACAGCAATGA CCTACAAGAC CCAGTGTTGA GTAGCGCCCA GGCCCAGCGC 
CTCATGCAGC TCATCTGCTA CCCACATCGG CTGCTGGACA ATGAGGATGG GGAAAATCCC 
CAGCGACAAC GCATTAAGCG TATTCTCCAG AACTTGGACC AGTGGACCAT GCGTCAGTCT 
TCCCTGGAGC TGCAGCTCAT GATCAAGCAG ACCCCTAACA ATGAGATGAA CTCCCTCTTA 
GAGAACATTG CCAAGGCCAC AATCGAGGTG TTCCAACAGT CAGCAGAGAC AGGGTCATCT 
TCTGGAAACA CTGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAGCCTGT GCTCAGCTCT 
CTGGAGCGCT CTGGTGTATG GCTGGTGGCC CCCCTCATTG CTAAACTGCC CACCTCAGTC 
CAGGGGCATG TGTTAAAGGC TGCTGGGGAG GAATTGGAGA AGGGCCAGCA CCTGGGTTCC 
TCTTCCCGCA AAGAACGTGA TCGACAAAAG CAAAAGAGTA TGTCCCTGTT GAGCCAGCAG 
CCTTTCTTGT CCCTGGTGCT GACGTGTCTG AAAGGGCAGG ATGAGCAGCG TGAGGGACTC 
CTTACCTCCC TTTACAGCCA AGTGCACCAG ATTGTGAATA ATTGGCGAGA TGACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC GGTGCAGCGC AGCACCCAAC AGACCACAGA GTGGGCTGTG 
CTCCTCCTGG AGATCATCAT CAGCGGCACT GTCGACATGC AGTCCAACAA CGAGCTCTTC 
ACCACTGTAT TGGACATGCT GAGCGTGCTC ATCAATGGGA CCCTGGCTGC GGACATGTCC 
AGCATCTCTC AAGGCAGCAT GGAGGAAAAC AAACGCGCCT ACATGAACCT GGTGAAGAAG 
CTGCGGAAAG AGTTGGGGGA GCGCCAATCA GACAGTCTGG AAAAAGTTCG CCAGCTGCTG 
CCACTGCCCA AGCAGACCCG AGATGTCATC ACATGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC CGGCTTTGAT TCCATCTTCA AGAAAGAGGG TCTACAGGTT 
TCCACCAAAC AAAAGATCTC TCCCTGGGAT CTTTTTGAGG GGCTGAAGCC GTCTGCACCA 
CTCTCTTGGG GCTGGTTTGG AACAGTCCGG GTCGACAGGC GAGTGGCCCG AGGCGAGGAG 
CAGCAGCGGT TGCTGCTCTA CCACACACAC CTGCGGCCCC GGCCCCGTGC CTATTACCTG 
GAGCCACTGC CACTGCCACC AGAGGATGAG GAGCCCCCCG CTCCCACCCC GCTAGAGCCT 
GAGAAAAAGG CTCCAGAGCC CCCCAAAACT GACAAGCCTG GGGCTGCTCC ACCTAGTACT 
GAGGAACGCA AGAAGAAGTC CACGAAGGGC AAGAAACGCA GCCAGCCTGC TGCCAAGACA 
GAGGACTATG GAATGGGCCC AGGGCGGAGT GGCCCCTATG GTGTGACCGT GCCTCCGGAC 
CTCCTGCACC ACGCCAACCC TGGTTCCATA TCCCACCTTA GCTACAGGCA GGGTTCCATA 
GGCCTGTACA CCCAGAACCA GCCACTACCT GCAGGTGGCC CTCGTGTGGA CCCATACCGC 
CCTATGCGGT TACCAATGCA GAAGCTGCCG ACCCGACCAC CTTACCCTGG AGTGCTACCC 
ACAGCCGTGA CTGGCGTCAT GGGACTGGAA CCCTCTTCCT ACAAGACCTC TGTGTACCGA 
CAGCAGCAGC CTGCGGTGCC CCAAGGACAG CGCCTTCGCC AACAGCTCCA GGCAAAGATA 
CAGAGTCAGG GGATGTTGGG ACAGTCATCT GTCCATCAGA TGACTCCCAG CTCTTCCTAC 
GGTTTGCAGA CCTCCCAGGG CTATACTCCT TATGTTTCTC ATGTGGGATT GCAGCAACAC 
ACAGGCCCCG CAGGTACCAT GGTGCCCCCC AGCTACTCCA GCCAGCCTTA TCAGAGCACC 
CACCCTTCTA CCAATCCTAC TCTTGTAGAT CCTACTCGCC ACCTGCAACA GCGGCCCAGT 
GGCTATGTGC ACCAGCAGGC CCCAACCTAC GGACATGGGC TGACCTCCAG TCAGAGGTTT 
TCACACCAGA CACTGCAGCA AACACCCATG ATGGGTACCA TGACTCCACT GGGCGCCCAG 
GGCGTCCAGG CCGGCGTCCG ATCAGCTTCC ATCTTGCCTG AGCAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAACAGCAGC AATACCACAT CCGGCAGCAG CAGCAGCAGC AGATCCTGCG GGTAAGGCCC 
TGGGATTTCC TCTGGGATCT GGGAGCCCAA GAAGGAGGGG AGGCACAAGC TCTTCCAGGT 
TGTTATGAAG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489436.1
CDS	55..6429
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633668.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633668.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA ACAAGCCTAC TGTAGGAATC 
CGCTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGAGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTTGGCGATG CGGAACTGAA GGGTTCAGGC 
TTCACTGTGA CAGGAGGAAC AGAAGAACTT CCAGAGGAGG AGGGGGGAGG TGGCAGTGGC 
GGTCGGAGGC AGGGTGGCCG CAACATCTCT GTGGAGACAG CCAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGCAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAATCG 
CTGTGTGAGG ACAGCAATGA CCTACAAGAC CCAGTGTTGA GTAGCGCCCA GGCCCAGCGC 
CTCATGCAGC TCATCTGCTA CCCACATCGG CTGCTGGACA ATGAGGATGG GGAAAATCCC 
CAGCGACAAC GCATTAAGCG TATTCTCCAG AACTTGGACC AGTGGACCAT GCGTCAGTCT 
TCCCTGGAGC TGCAGCTCAT GATCAAGCAG ACCCCTAACA ATGAGATGAA CTCCCTCTTA 
GAGAACATTG CCAAGGCCAC AATCGAGGTG TTCCAACAGT CAGCAGAGAC AGGGTCATCT 
TCTGGAAACA CTGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAGCCTGT GCTCAGCTCT 
CTGGAGCGCT CTGGTGTATG GCTGGTGGCC CCCCTCATTG CTAAACTGCC CACCTCAGTC 
CAGGGGCATG TGTTAAAGGC TGCTGGGGAG GAATTGGAGA AGGGCCAGCA CCTGGGTTCC 
TCTTCCCGCA AAGAACGTGA TCGACAAAAG CAAAAGAGTA TGTCCCTGTT GAGCCAGCAG 
CCTTTCTTGT CCCTGGTGCT GACGTGTCTG AAAGGGCAGG ATGAGCAGCG TGAGGGACTC 
CTTACCTCCC TTTACAGCCA AGTGCACCAG ATTGTGAATA ATTGGCGAGA TGACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC GGTGCAGCGC AGCACCCAAC AGACCACAGA GTGGGCTGTG 
CTCCTCCTGG AGATCATCAT CAGCGGCACT GTCGACATGC AGTCCAACAA CGAGCTCTTC 
ACCACTGTAT TGGACATGCT GAGCGTGCTC ATCAATGGGA CCCTGGCTGC GGACATGTCC 
AGCATCTCTC AAGGCAGCAT GGAGGAAAAC AAACGCGCCT ACATGAACCT GGTGAAGAAG 
CTGCGGAAAG AGTTGGGGGA GCGCCAATCA GACAGTCTGG AAAAAGTTCG CCAGCTGCTG 
CCACTGCCCA AGCAGACCCG AGATGTCATC ACATGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC CGGCTTTGAT TCCATCTTCA AGAAAGAGGG TCTACAGGTT 
TCCACCAAAC AAAAGATCTC TCCCTGGGAT CTTTTTGAGG GGCTGAAGCC GTCTGCACCA 
CTCTCTTGGG GCTGGTTTGG AACAGTCCGG GTCGACAGGC GAGTGGCCCG AGGCGAGGAG 
CAGCAGCGGT TGCTGCTCTA CCACACACAC CTGCGGCCCC GGCCCCGTGC CTATTACCTG 
GAGCCACTGC CACTGCCACC AGAGGATGAG GAGCCCCCCG CTCCCACCCC GCTAGAGCCT 
GAGAAAAAGG CTCCAGAGCC CCCCAAAACT GACAAGCCTG GGGCTGCTCC ACCTAGTACT 
GAGGAACGCA AGAAGAAGTC CACGAAGGGC AAGAAACGCA GCCAGCCTGC TGCCAAGACA 
GAGGACTATG GAATGGGCCC AGGGCGGAGT GGCCCCTATG GTGTGACCGT GCCTCCGGAC 
CTCCTGCACC ACGCCAACCC TGGTTCCATA TCCCACCTTA GCTACAGGCA GGGTTCCATA 
GGCCTGTACA CCCAGAACCA GCCACTACCT GCAGGTGGCC CTCGTGTGGA CCCATACCGC 
CCTATGCGGT TACCAATGCA GAAGCTGCCG ACCCGACCAC CTTACCCTGG AGTGCTACCC 
ACAGCCGTGA CTGGCGTCAT GGGACTGGAA CCCTCTTCCT ACAAGACCTC TGTGTACCGA 
CAGCAGCAGC CTGCGGTGCC CCAAGGACAG CGCCTTCGCC AACAGCTCCA GGCAAAGATA 
CAGAGTCAGG GGATGTTGGG ACAGTCATCT GTCCATCAGA TGACTCCCAG CTCTTCCTAC 
GGTTTGCAGA CCTCCCAGGG CTATACTCCT TATGTTTCTC ATGTGGGATT GCAGCAACAC 
ACAGGCCCCG CAGGTACCAT GGTGCCCCCC AGCTACTCCA GCCAGCCTTA TCAGAGCACC 
CACCCTTCTA CCAATCCTAC TCTTGTAGAT CCTACTCGCC ACCTGCAACA GCGGCCCAGT 
GGCTATGTGC ACCAGCAGGC CCCAACCTAC GGACATGGGC TGACCTCCAG TCAGAGGTTT 
TCACACCAGA CACTGCAGCA AACACCCATG ATGGGTACCA TGACTCCACT GGGCGCCCAG 
GGCGTCCAGG CCGGCGTCCG ATCAGCTTCC ATCTTGCCTG AGCAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAACAGCAGC AATACCACAT CCGGCAGCAG CAGCAGCAGC AGATCCTGCG GGTAAGGCCC 
TGGGATTTCC TCTGGGATCT GGGAGCCCAA GAAGGAGGGG AGGCACAAGC TCTTCCAGGT 
TGTTATGAAG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633670.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489438.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X5, mRNA.

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CloneID	OHb71701
Clone ID Related Accession (Same CDS sequence)	XM_023633670.1
Accession Version	XM_023633670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGGTGGGG AGGATGGGCA GAAGCGACGG 
CGCAACCGGC CTGAAGCCTT CCCCACTGCT GAAGATATCT TTGCTAAGTT CCAGCATCTT 
TCACATTATG ACCAACACCA GGTCACGGCT CAGGTCTCCC GGAATGTTCT GGAGCAGATC 
ACGAGTTTTG CCCTTGGCAT GTCATACCAC TTGCCTCTGG TGCAGCATGT GCAGTTCATC 
TTCGACCTCA TGGAATATTC ACTCAGCATC AGTGGCCTCA TCGACTTTGC CATTCAGCTA 
CTGAATGAAC TGAGTGTAGT TGAGGCTGAG TTGCTTCTCA AATCCTCGGA TCTGGTGGGC 
AGCTACACTA CCAGCCTGTG CCTGTGCATT GTGGCTGTCC TGCGGCACTA TCATGCCTGC 
CTCATCCTCA ACCAGGACCA GATGGCACAG GTCTTTGAGG GGCTGTGTGG CGTAGTGAAG 
CATGGGATGA ACCGGTCAGA TGGCTCCTCT GCAGAACGCT GTATCCTTGC TTATCTCTAT 
GATCTGTACA CCTCCTGTAG CCATTTAAAG AGCAAATTTG GGGAGCTCTT CAGCGACTTC 
TGCTCCAAGG TGAAGAACAC CATCTACTGC AACGTGGAGC CGTCAGAATC CAATATGCGC 
TGGGCACCTG AGTTCATGAT TGACACTCTG GAGAACCCTG CAGCTCACAC CTTCACCTAC 
ACGGGGCTAG GCAAGAGTCT TAGTGAGAAC CCTGCTAACC GCTACAGCTT TGTCTGCAAT 
GCCCTTATGC ACGTCTGTGT GGGGCACCAT GATCCCGATA GGGTGAATGA CATCGCAATC 
CTGTGTGCAG AGCTGACCGG CTATTGCAAG TCACTGAGTG CGGAATGGCT AGGAGTCCTT 
AAAGCCTTGT GCTGCTCCTC TAACAATGGC ACTTGTGGTT TCAACGACCT CCTCTGCAAT 
GTAGATGTCA GTGACCTGTC TTTTCATGAT TCCCTGGCTA CTTTTGTTGC CATCCTCATC 
GCTCGGCAGT GTTTGCTCCT AGAGGATCTG ATTCGCTGTG CTGCCATCCC TTCACTCCTT 
AATGCTGCTT GCAGTGAGCA GGATTCTGAG CCCGGGGCCC GACTTACCTG CCGCATCCTC 
CTTCACCTTT TCAAGACACC TCAACTCAAT CCTTGCCAGT CGGATGGAAC TCCTTCCCCA 
GACAAGCCTA CTGTAGGAAT CCGCTCCTCC TGTGACCGCC ACCTGCTGGC TGCCTCCCAG 
AACCGCATCG TGGATGGAGC TGTGTTTGCT GTTCTCAAGG CTGTGTTTGT ACTTGGCGAT 
GCGGAACTGA AGGGTTCAGG CTTCACTGTG ACAGGAGGAA CAGAAGAACT TCCAGAGGAG 
GAGGGGGGAG GTGGCAGTGG CGGTCGGAGG CAGGGTGGCC GCAACATCTC TGTGGAGACA 
GCCAGTCTGG ATGTCTATGC CAAGTACGTG CTGCGCAGCA TCTGCCAACA GGAATGGGTA 
GGAGAACGTT GCCTTAAATC GCTGTGTGAG GACAGCAATG ACCTACAAGA CCCAGTGTTG 
AGTAGCGCCC AGGCCCAGCG CCTCATGCAG CTCATCTGCT ACCCACATCG GCTGCTGGAC 
AATGAGGATG GGGAAAATCC CCAGCGACAA CGCATTAAGC GTATTCTCCA GAACTTGGAC 
CAGTGGACCA TGCGTCAGTC TTCCCTGGAG CTGCAGCTCA TGATCAAGCA GACCCCTAAC 
AATGAGATGA ACTCCCTCTT AGAGAACATT GCCAAGGCCA CAATCGAGGT GTTCCAACAG 
TCAGCAGAGA CAGGGTCATC TTCTGGAAAC ACTGCAAGCA ACATGCCCAG CAGCAGCAAG 
ACCAAGCCTG TGCTCAGCTC TCTGGAGCGC TCTGGTGTAT GGCTGGTGGC CCCCCTCATT 
GCTAAACTGC CCACCTCAGT CCAGGGGCAT GTGTTAAAGG CTGCTGGGGA GGAATTGGAG 
AAGGGCCAGC ACCTGGGTTC CTCTTCCCGC AAAGAACGTG ATCGACAAAA GCAAAAGAGT 
ATGTCCCTGT TGAGCCAGCA GCCTTTCTTG TCCCTGGTGC TGACGTGTCT GAAAGGGCAG 
GATGAGCAGC GTGAGGGACT CCTTACCTCC CTTTACAGCC AAGTGCACCA GATTGTGAAT 
AATTGGCGAG ATGACCAGTA CTTAGATGAT TGCAAACCAA AGCAGCTAAT GCATGAGGCA 
CTCAAACTGC GGCTCAACCT GGTGGGGGGC ATGTTTGACA CGGTGCAGCG CAGCACCCAA 
CAGACCACAG AGTGGGCTGT GCTCCTCCTG GAGATCATCA TCAGCGGCAC TGTCGACATG 
CAGTCCAACA ACGAGCTCTT CACCACTGTA TTGGACATGC TGAGCGTGCT CATCAATGGG 
ACCCTGGCTG CGGACATGTC CAGCATCTCT CAAGGCAGCA TGGAGGAAAA CAAACGCGCC 
TACATGAACC TGGTGAAGAA GCTGCGGAAA GAGTTGGGGG AGCGCCAATC AGACAGTCTG 
GAAAAAGTTC GCCAGCTGCT GCCACTGCCC AAGCAGACCC GAGATGTCAT CACATGTGAG 
CCACAGGGCT CCCTTATTGA CACCAAGGGC AACAAGATTG CCGGCTTTGA TTCCATCTTC 
AAGAAAGAGG GTCTACAGGT TTCCACCAAA CAAAAGATCT CTCCCTGGGA TCTTTTTGAG 
GGGCTGAAGC CGTCTGCACC ACTCTCTTGG GGCTGGTTTG GAACAGTCCG GGTCGACAGG 
CGAGTGGCCC GAGGCGAGGA GCAGCAGCGG TTGCTGCTCT ACCACACACA CCTGCGGCCC 
CGGCCCCGTG CCTATTACCT GGAGCCACTG CCACTGCCAC CAGAGGATGA GGAGCCCCCC 
GCTCCCACCC CGCTAGAGCC TGAGAAAAAG GCTCCAGAGC CCCCCAAAAC TGACAAGCCT 
GGGGCTGCTC CACCTAGTAC TGAGGAACGC AAGAAGAAGT CCACGAAGGG CAAGAAACGC 
AGCCAGCCTG CTGCCAAGAC AGAGGACTAT GGAATGGGCC CAGGGCGGAG TGGCCCCTAT 
GGTGTGACCG TGCCTCCGGA CCTCCTGCAC CACGCCAACC CTGGTTCCAT ATCCCACCTT 
AGCTACAGGC AGGGTTCCAT AGGCCTGTAC ACCCAGAACC AGCCACTACC TGCAGGTGGC 
CCTCGTGTGG ACCCATACCG CCCTATGCGG TTACCAATGC AGAAGCTGCC GACCCGACCA 
CCTTACCCTG GAGTGCTACC CACAGCCGTG ACTGGCGTCA TGGGACTGGA ACCCTCTTCC 
TACAAGACCT CTGTGTACCG ACAGCAGCAG CCTGCGGTGC CCCAAGGACA GCGCCTTCGC 
CAACAGCTCC AGGCAAAGAT ACAGAGTCAG GGGATGTTGG GACAGTCATC TGTCCATCAG 
ATGACTCCCA GCTCTTCCTA CGGTTTGCAG ACCTCCCAGG GCTATACTCC TTATGTTTCT 
CATGTGGGAT TGCAGCAACA CACAGGCCCC GCAGGTACCA TGGTGCCCCC CAGCTACTCC 
AGCCAGCCTT ATCAGAGCAC CCACCCTTCT ACCAATCCTA CTCTTGTAGA TCCTACTCGC 
CACCTGCAAC AGCGGCCCAG TGGCTATGTG CACCAGCAGG CCCCAACCTA CGGACATGGG 
CTGACCTCCA GTCAGAGGTT TTCACACCAG ACACTGCAGC AAACACCCAT GATGGGTACC 
ATGACTCCAC TGGGCGCCCA GGGCGTCCAG GCCGGCGTCC GATCAGCTTC CATCTTGCCT 
GAGCAGCAGC AACAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAACAGCAG CAATACCACA TCCGGCAGCA GCAGCAGCAG 
CAGATCCTGC GGGTAAGGCC CTGGGATTTC CTCTGGGATC TGGGAGCCCA AGAAGGAGGG 
GAGGCACAAG CTCTTCCAGG TTGTTATGAA GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489438.1
CDS	56..6391
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGTPSPDKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633670.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633670.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGGTGGGG AGGATGGGCA GAAGCGACGG 
CGCAACCGGC CTGAAGCCTT CCCCACTGCT GAAGATATCT TTGCTAAGTT CCAGCATCTT 
TCACATTATG ACCAACACCA GGTCACGGCT CAGGTCTCCC GGAATGTTCT GGAGCAGATC 
ACGAGTTTTG CCCTTGGCAT GTCATACCAC TTGCCTCTGG TGCAGCATGT GCAGTTCATC 
TTCGACCTCA TGGAATATTC ACTCAGCATC AGTGGCCTCA TCGACTTTGC CATTCAGCTA 
CTGAATGAAC TGAGTGTAGT TGAGGCTGAG TTGCTTCTCA AATCCTCGGA TCTGGTGGGC 
AGCTACACTA CCAGCCTGTG CCTGTGCATT GTGGCTGTCC TGCGGCACTA TCATGCCTGC 
CTCATCCTCA ACCAGGACCA GATGGCACAG GTCTTTGAGG GGCTGTGTGG CGTAGTGAAG 
CATGGGATGA ACCGGTCAGA TGGCTCCTCT GCAGAACGCT GTATCCTTGC TTATCTCTAT 
GATCTGTACA CCTCCTGTAG CCATTTAAAG AGCAAATTTG GGGAGCTCTT CAGCGACTTC 
TGCTCCAAGG TGAAGAACAC CATCTACTGC AACGTGGAGC CGTCAGAATC CAATATGCGC 
TGGGCACCTG AGTTCATGAT TGACACTCTG GAGAACCCTG CAGCTCACAC CTTCACCTAC 
ACGGGGCTAG GCAAGAGTCT TAGTGAGAAC CCTGCTAACC GCTACAGCTT TGTCTGCAAT 
GCCCTTATGC ACGTCTGTGT GGGGCACCAT GATCCCGATA GGGTGAATGA CATCGCAATC 
CTGTGTGCAG AGCTGACCGG CTATTGCAAG TCACTGAGTG CGGAATGGCT AGGAGTCCTT 
AAAGCCTTGT GCTGCTCCTC TAACAATGGC ACTTGTGGTT TCAACGACCT CCTCTGCAAT 
GTAGATGTCA GTGACCTGTC TTTTCATGAT TCCCTGGCTA CTTTTGTTGC CATCCTCATC 
GCTCGGCAGT GTTTGCTCCT AGAGGATCTG ATTCGCTGTG CTGCCATCCC TTCACTCCTT 
AATGCTGCTT GCAGTGAGCA GGATTCTGAG CCCGGGGCCC GACTTACCTG CCGCATCCTC 
CTTCACCTTT TCAAGACACC TCAACTCAAT CCTTGCCAGT CGGATGGAAC TCCTTCCCCA 
GACAAGCCTA CTGTAGGAAT CCGCTCCTCC TGTGACCGCC ACCTGCTGGC TGCCTCCCAG 
AACCGCATCG TGGATGGAGC TGTGTTTGCT GTTCTCAAGG CTGTGTTTGT ACTTGGCGAT 
GCGGAACTGA AGGGTTCAGG CTTCACTGTG ACAGGAGGAA CAGAAGAACT TCCAGAGGAG 
GAGGGGGGAG GTGGCAGTGG CGGTCGGAGG CAGGGTGGCC GCAACATCTC TGTGGAGACA 
GCCAGTCTGG ATGTCTATGC CAAGTACGTG CTGCGCAGCA TCTGCCAACA GGAATGGGTA 
GGAGAACGTT GCCTTAAATC GCTGTGTGAG GACAGCAATG ACCTACAAGA CCCAGTGTTG 
AGTAGCGCCC AGGCCCAGCG CCTCATGCAG CTCATCTGCT ACCCACATCG GCTGCTGGAC 
AATGAGGATG GGGAAAATCC CCAGCGACAA CGCATTAAGC GTATTCTCCA GAACTTGGAC 
CAGTGGACCA TGCGTCAGTC TTCCCTGGAG CTGCAGCTCA TGATCAAGCA GACCCCTAAC 
AATGAGATGA ACTCCCTCTT AGAGAACATT GCCAAGGCCA CAATCGAGGT GTTCCAACAG 
TCAGCAGAGA CAGGGTCATC TTCTGGAAAC ACTGCAAGCA ACATGCCCAG CAGCAGCAAG 
ACCAAGCCTG TGCTCAGCTC TCTGGAGCGC TCTGGTGTAT GGCTGGTGGC CCCCCTCATT 
GCTAAACTGC CCACCTCAGT CCAGGGGCAT GTGTTAAAGG CTGCTGGGGA GGAATTGGAG 
AAGGGCCAGC ACCTGGGTTC CTCTTCCCGC AAAGAACGTG ATCGACAAAA GCAAAAGAGT 
ATGTCCCTGT TGAGCCAGCA GCCTTTCTTG TCCCTGGTGC TGACGTGTCT GAAAGGGCAG 
GATGAGCAGC GTGAGGGACT CCTTACCTCC CTTTACAGCC AAGTGCACCA GATTGTGAAT 
AATTGGCGAG ATGACCAGTA CTTAGATGAT TGCAAACCAA AGCAGCTAAT GCATGAGGCA 
CTCAAACTGC GGCTCAACCT GGTGGGGGGC ATGTTTGACA CGGTGCAGCG CAGCACCCAA 
CAGACCACAG AGTGGGCTGT GCTCCTCCTG GAGATCATCA TCAGCGGCAC TGTCGACATG 
CAGTCCAACA ACGAGCTCTT CACCACTGTA TTGGACATGC TGAGCGTGCT CATCAATGGG 
ACCCTGGCTG CGGACATGTC CAGCATCTCT CAAGGCAGCA TGGAGGAAAA CAAACGCGCC 
TACATGAACC TGGTGAAGAA GCTGCGGAAA GAGTTGGGGG AGCGCCAATC AGACAGTCTG 
GAAAAAGTTC GCCAGCTGCT GCCACTGCCC AAGCAGACCC GAGATGTCAT CACATGTGAG 
CCACAGGGCT CCCTTATTGA CACCAAGGGC AACAAGATTG CCGGCTTTGA TTCCATCTTC 
AAGAAAGAGG GTCTACAGGT TTCCACCAAA CAAAAGATCT CTCCCTGGGA TCTTTTTGAG 
GGGCTGAAGC CGTCTGCACC ACTCTCTTGG GGCTGGTTTG GAACAGTCCG GGTCGACAGG 
CGAGTGGCCC GAGGCGAGGA GCAGCAGCGG TTGCTGCTCT ACCACACACA CCTGCGGCCC 
CGGCCCCGTG CCTATTACCT GGAGCCACTG CCACTGCCAC CAGAGGATGA GGAGCCCCCC 
GCTCCCACCC CGCTAGAGCC TGAGAAAAAG GCTCCAGAGC CCCCCAAAAC TGACAAGCCT 
GGGGCTGCTC CACCTAGTAC TGAGGAACGC AAGAAGAAGT CCACGAAGGG CAAGAAACGC 
AGCCAGCCTG CTGCCAAGAC AGAGGACTAT GGAATGGGCC CAGGGCGGAG TGGCCCCTAT 
GGTGTGACCG TGCCTCCGGA CCTCCTGCAC CACGCCAACC CTGGTTCCAT ATCCCACCTT 
AGCTACAGGC AGGGTTCCAT AGGCCTGTAC ACCCAGAACC AGCCACTACC TGCAGGTGGC 
CCTCGTGTGG ACCCATACCG CCCTATGCGG TTACCAATGC AGAAGCTGCC GACCCGACCA 
CCTTACCCTG GAGTGCTACC CACAGCCGTG ACTGGCGTCA TGGGACTGGA ACCCTCTTCC 
TACAAGACCT CTGTGTACCG ACAGCAGCAG CCTGCGGTGC CCCAAGGACA GCGCCTTCGC 
CAACAGCTCC AGGCAAAGAT ACAGAGTCAG GGGATGTTGG GACAGTCATC TGTCCATCAG 
ATGACTCCCA GCTCTTCCTA CGGTTTGCAG ACCTCCCAGG GCTATACTCC TTATGTTTCT 
CATGTGGGAT TGCAGCAACA CACAGGCCCC GCAGGTACCA TGGTGCCCCC CAGCTACTCC 
AGCCAGCCTT ATCAGAGCAC CCACCCTTCT ACCAATCCTA CTCTTGTAGA TCCTACTCGC 
CACCTGCAAC AGCGGCCCAG TGGCTATGTG CACCAGCAGG CCCCAACCTA CGGACATGGG 
CTGACCTCCA GTCAGAGGTT TTCACACCAG ACACTGCAGC AAACACCCAT GATGGGTACC 
ATGACTCCAC TGGGCGCCCA GGGCGTCCAG GCCGGCGTCC GATCAGCTTC CATCTTGCCT 
GAGCAGCAGC AACAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAACAGCAG CAATACCACA TCCGGCAGCA GCAGCAGCAG 
CAGATCCTGC GGGTAAGGCC CTGGGATTTC CTCTGGGATC TGGGAGCCCA AGAAGGAGGG 
GAGGCACAAG CTCTTCCAGG TTGTTATGAA GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633671.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489439.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X6, mRNA.

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CloneID	OHb71702
Clone ID Related Accession (Same CDS sequence)	XM_023633671.1
Accession Version	XM_023633671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGGTGGGG AGGATGGGCA GAAGCGACGG 
CGCAACCGGC CTGAAGCCTT CCCCACTGCT GAAGATATCT TTGCTAAGTT CCAGCATCTT 
TCACATTATG ACCAACACCA GGTCACGGCT CAGGTCTCCC GGAATGTTCT GGAGCAGATC 
ACGAGTTTTG CCCTTGGCAT GTCATACCAC TTGCCTCTGG TGCAGCATGT GCAGTTCATC 
TTCGACCTCA TGGAATATTC ACTCAGCATC AGTGGCCTCA TCGACTTTGC CATTCAGCTA 
CTGAATGAAC TGAGTGTAGT TGAGGCTGAG TTGCTTCTCA AATCCTCGGA TCTGGTGGGC 
AGCTACACTA CCAGCCTGTG CCTGTGCATT GTGGCTGTCC TGCGGCACTA TCATGCCTGC 
CTCATCCTCA ACCAGGACCA GATGGCACAG GTCTTTGAGG GGCTGTGTGG CGTAGTGAAG 
CATGGGATGA ACCGGTCAGA TGGCTCCTCT GCAGAACGCT GTATCCTTGC TTATCTCTAT 
GATCTGTACA CCTCCTGTAG CCATTTAAAG AGCAAATTTG GGGAGCTCTT CAGCGACTTC 
TGCTCCAAGG TGAAGAACAC CATCTACTGC AACGTGGAGC CGTCAGAATC CAATATGCGC 
TGGGCACCTG AGTTCATGAT TGACACTCTG GAGAACCCTG CAGCTCACAC CTTCACCTAC 
ACGGGGCTAG GCAAGAGTCT TAGTGAGAAC CCTGCTAACC GCTACAGCTT TGTCTGCAAT 
GCCCTTATGC ACGTCTGTGT GGGGCACCAT GATCCCGATA GGGTGAATGA CATCGCAATC 
CTGTGTGCAG AGCTGACCGG CTATTGCAAG TCACTGAGTG CGGAATGGCT AGGAGTCCTT 
AAAGCCTTGT GCTGCTCCTC TAACAATGGC ACTTGTGGTT TCAACGACCT CCTCTGCAAT 
GTAGATGTCA GTGACCTGTC TTTTCATGAT TCCCTGGCTA CTTTTGTTGC CATCCTCATC 
GCTCGGCAGT GTTTGCTCCT AGAGGATCTG ATTCGCTGTG CTGCCATCCC TTCACTCCTT 
AATGCTGCTT GCAGTGAGCA GGATTCTGAG CCCGGGGCCC GACTTACCTG CCGCATCCTC 
CTTCACCTTT TCAAGACACC TCAACTCAAT CCTTGCCAGT CGGATGGAAA CAAGCCTACT 
GTAGGAATCC GCTCCTCCTG TGACCGCCAC CTGCTGGCTG CCTCCCAGAA CCGCATCGTG 
GATGGAGCTG TGTTTGCTGT TCTCAAGGCT GTGTTTGTAC TTGGCGATGC GGAACTGAAG 
GGTTCAGGCT TCACTGTGAC AGGAGGAACA GAAGAACTTC CAGAGGAGGA GGGGGGAGGT 
GGCAGTGGCG GTCGGAGGCA GGGTGGCCGC AACATCTCTG TGGAGACAGC CAGTCTGGAT 
GTCTATGCCA AGTACGTGCT GCGCAGCATC TGCCAACAGG AATGGGTAGG AGAACGTTGC 
CTTAAATCGC TGTGTGAGGA CAGCAATGAC CTACAAGACC CAGTGTTGAG TAGCGCCCAG 
GCCCAGCGCC TCATGCAGCT CATCTGCTAC CCACATCGGC TGCTGGACAA TGAGGATGGG 
GAAAATCCCC AGCGACAACG CATTAAGCGT ATTCTCCAGA ACTTGGACCA GTGGACCATG 
CGTCAGTCTT CCCTGGAGCT GCAGCTCATG ATCAAGCAGA CCCCTAACAA TGAGATGAAC 
TCCCTCTTAG AGAACATTGC CAAGGCCACA ATCGAGGTGT TCCAACAGTC AGCAGAGACA 
GGGTCATCTT CTGGAAACAC TGCAAGCAAC ATGCCCAGCA GCAGCAAGAC CAAGCCTGTG 
CTCAGCTCTC TGGAGCGCTC TGGTGTATGG CTGGTGGCCC CCCTCATTGC TAAACTGCCC 
ACCTCAGTCC AGGGGCATGT GTTAAAGGCT GCTGGGGAGG AATTGGAGAA GGGCCAGCAC 
CTGGGTTCCT CTTCCCGCAA AGAACGTGAT CGACAAAAGC AAAAGAGTAT GTCCCTGTTG 
AGCCAGCAGC CTTTCTTGTC CCTGGTGCTG ACGTGTCTGA AAGGGCAGGA TGAGCAGCGT 
GAGGGACTCC TTACCTCCCT TTACAGCCAA GTGCACCAGA TTGTGAATAA TTGGCGAGAT 
GACCAGTACT TAGATGATTG CAAACCAAAG CAGCTAATGC ATGAGGCACT CAAACTGCGG 
CTCAACCTGG TGGGGGGCAT GTTTGACACG GTGCAGCGCA GCACCCAACA GACCACAGAG 
TGGGCTGTGC TCCTCCTGGA GATCATCATC AGCGGCACTG TCGACATGCA GTCCAACAAC 
GAGCTCTTCA CCACTGTATT GGACATGCTG AGCGTGCTCA TCAATGGGAC CCTGGCTGCG 
GACATGTCCA GCATCTCTCA AGGCAGCATG GAGGAAAACA AACGCGCCTA CATGAACCTG 
GTGAAGAAGC TGCGGAAAGA GTTGGGGGAG CGCCAATCAG ACAGTCTGGA AAAAGTTCGC 
CAGCTGCTGC CACTGCCCAA GCAGACCCGA GATGTCATCA CATGTGAGCC ACAGGGCTCC 
CTTATTGACA CCAAGGGCAA CAAGATTGCC GGCTTTGATT CCATCTTCAA GAAAGAGGGT 
CTACAGGTTT CCACCAAACA AAAGATCTCT CCCTGGGATC TTTTTGAGGG GCTGAAGCCG 
TCTGCACCAC TCTCTTGGGG CTGGTTTGGA ACAGTCCGGG TCGACAGGCG AGTGGCCCGA 
GGCGAGGAGC AGCAGCGGTT GCTGCTCTAC CACACACACC TGCGGCCCCG GCCCCGTGCC 
TATTACCTGG AGCCACTGCC ACTGCCACCA GAGGATGAGG AGCCCCCCGC TCCCACCCCG 
CTAGAGCCTG AGAAAAAGGC TCCAGAGCCC CCCAAAACTG ACAAGCCTGG GGCTGCTCCA 
CCTAGTACTG AGGAACGCAA GAAGAAGTCC ACGAAGGGCA AGAAACGCAG CCAGCCTGCT 
GCCAAGACAG AGGACTATGG AATGGGCCCA GGGCGGAGTG GCCCCTATGG TGTGACCGTG 
CCTCCGGACC TCCTGCACCA CGCCAACCCT GGTTCCATAT CCCACCTTAG CTACAGGCAG 
GGTTCCATAG GCCTGTACAC CCAGAACCAG CCACTACCTG CAGGTGGCCC TCGTGTGGAC 
CCATACCGCC CTATGCGGTT ACCAATGCAG AAGCTGCCGA CCCGACCACC TTACCCTGGA 
GTGCTACCCA CAGCCGTGAC TGGCGTCATG GGACTGGAAC CCTCTTCCTA CAAGACCTCT 
GTGTACCGAC AGCAGCAGCC TGCGGTGCCC CAAGGACAGC GCCTTCGCCA ACAGCTCCAG 
GCAAAGATAC AGAGTCAGGG GATGTTGGGA CAGTCATCTG TCCATCAGAT GACTCCCAGC 
TCTTCCTACG GTTTGCAGAC CTCCCAGGGC TATACTCCTT ATGTTTCTCA TGTGGGATTG 
CAGCAACACA CAGGCCCCGC AGGTACCATG GTGCCCCCCA GCTACTCCAG CCAGCCTTAT 
CAGAGCACCC ACCCTTCTAC CAATCCTACT CTTGTAGATC CTACTCGCCA CCTGCAACAG 
CGGCCCAGTG GCTATGTGCA CCAGCAGGCC CCAACCTACG GACATGGGCT GACCTCCAGT 
CAGAGGTTTT CACACCAGAC ACTGCAGCAA ACACCCATGA TGGGTACCAT GACTCCACTG 
GGCGCCCAGG GCGTCCAGGC CGGCGTCCGA TCAGCTTCCA TCTTGCCTGA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGCAGCA ATACCACATC CGGCAGCAGC AGCAGCAGCA GATCCTGCGG 
GTAAGGCCCT GGGATTTCCT CTGGGATCTG GGAGCCCAAG AAGGAGGGGA GGCACAAGCT 
CTTCCAGGTT GTTATGAAGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489439.1
CDS	55..6378
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633671.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633671.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGGTGGGG AGGATGGGCA GAAGCGACGG 
CGCAACCGGC CTGAAGCCTT CCCCACTGCT GAAGATATCT TTGCTAAGTT CCAGCATCTT 
TCACATTATG ACCAACACCA GGTCACGGCT CAGGTCTCCC GGAATGTTCT GGAGCAGATC 
ACGAGTTTTG CCCTTGGCAT GTCATACCAC TTGCCTCTGG TGCAGCATGT GCAGTTCATC 
TTCGACCTCA TGGAATATTC ACTCAGCATC AGTGGCCTCA TCGACTTTGC CATTCAGCTA 
CTGAATGAAC TGAGTGTAGT TGAGGCTGAG TTGCTTCTCA AATCCTCGGA TCTGGTGGGC 
AGCTACACTA CCAGCCTGTG CCTGTGCATT GTGGCTGTCC TGCGGCACTA TCATGCCTGC 
CTCATCCTCA ACCAGGACCA GATGGCACAG GTCTTTGAGG GGCTGTGTGG CGTAGTGAAG 
CATGGGATGA ACCGGTCAGA TGGCTCCTCT GCAGAACGCT GTATCCTTGC TTATCTCTAT 
GATCTGTACA CCTCCTGTAG CCATTTAAAG AGCAAATTTG GGGAGCTCTT CAGCGACTTC 
TGCTCCAAGG TGAAGAACAC CATCTACTGC AACGTGGAGC CGTCAGAATC CAATATGCGC 
TGGGCACCTG AGTTCATGAT TGACACTCTG GAGAACCCTG CAGCTCACAC CTTCACCTAC 
ACGGGGCTAG GCAAGAGTCT TAGTGAGAAC CCTGCTAACC GCTACAGCTT TGTCTGCAAT 
GCCCTTATGC ACGTCTGTGT GGGGCACCAT GATCCCGATA GGGTGAATGA CATCGCAATC 
CTGTGTGCAG AGCTGACCGG CTATTGCAAG TCACTGAGTG CGGAATGGCT AGGAGTCCTT 
AAAGCCTTGT GCTGCTCCTC TAACAATGGC ACTTGTGGTT TCAACGACCT CCTCTGCAAT 
GTAGATGTCA GTGACCTGTC TTTTCATGAT TCCCTGGCTA CTTTTGTTGC CATCCTCATC 
GCTCGGCAGT GTTTGCTCCT AGAGGATCTG ATTCGCTGTG CTGCCATCCC TTCACTCCTT 
AATGCTGCTT GCAGTGAGCA GGATTCTGAG CCCGGGGCCC GACTTACCTG CCGCATCCTC 
CTTCACCTTT TCAAGACACC TCAACTCAAT CCTTGCCAGT CGGATGGAAA CAAGCCTACT 
GTAGGAATCC GCTCCTCCTG TGACCGCCAC CTGCTGGCTG CCTCCCAGAA CCGCATCGTG 
GATGGAGCTG TGTTTGCTGT TCTCAAGGCT GTGTTTGTAC TTGGCGATGC GGAACTGAAG 
GGTTCAGGCT TCACTGTGAC AGGAGGAACA GAAGAACTTC CAGAGGAGGA GGGGGGAGGT 
GGCAGTGGCG GTCGGAGGCA GGGTGGCCGC AACATCTCTG TGGAGACAGC CAGTCTGGAT 
GTCTATGCCA AGTACGTGCT GCGCAGCATC TGCCAACAGG AATGGGTAGG AGAACGTTGC 
CTTAAATCGC TGTGTGAGGA CAGCAATGAC CTACAAGACC CAGTGTTGAG TAGCGCCCAG 
GCCCAGCGCC TCATGCAGCT CATCTGCTAC CCACATCGGC TGCTGGACAA TGAGGATGGG 
GAAAATCCCC AGCGACAACG CATTAAGCGT ATTCTCCAGA ACTTGGACCA GTGGACCATG 
CGTCAGTCTT CCCTGGAGCT GCAGCTCATG ATCAAGCAGA CCCCTAACAA TGAGATGAAC 
TCCCTCTTAG AGAACATTGC CAAGGCCACA ATCGAGGTGT TCCAACAGTC AGCAGAGACA 
GGGTCATCTT CTGGAAACAC TGCAAGCAAC ATGCCCAGCA GCAGCAAGAC CAAGCCTGTG 
CTCAGCTCTC TGGAGCGCTC TGGTGTATGG CTGGTGGCCC CCCTCATTGC TAAACTGCCC 
ACCTCAGTCC AGGGGCATGT GTTAAAGGCT GCTGGGGAGG AATTGGAGAA GGGCCAGCAC 
CTGGGTTCCT CTTCCCGCAA AGAACGTGAT CGACAAAAGC AAAAGAGTAT GTCCCTGTTG 
AGCCAGCAGC CTTTCTTGTC CCTGGTGCTG ACGTGTCTGA AAGGGCAGGA TGAGCAGCGT 
GAGGGACTCC TTACCTCCCT TTACAGCCAA GTGCACCAGA TTGTGAATAA TTGGCGAGAT 
GACCAGTACT TAGATGATTG CAAACCAAAG CAGCTAATGC ATGAGGCACT CAAACTGCGG 
CTCAACCTGG TGGGGGGCAT GTTTGACACG GTGCAGCGCA GCACCCAACA GACCACAGAG 
TGGGCTGTGC TCCTCCTGGA GATCATCATC AGCGGCACTG TCGACATGCA GTCCAACAAC 
GAGCTCTTCA CCACTGTATT GGACATGCTG AGCGTGCTCA TCAATGGGAC CCTGGCTGCG 
GACATGTCCA GCATCTCTCA AGGCAGCATG GAGGAAAACA AACGCGCCTA CATGAACCTG 
GTGAAGAAGC TGCGGAAAGA GTTGGGGGAG CGCCAATCAG ACAGTCTGGA AAAAGTTCGC 
CAGCTGCTGC CACTGCCCAA GCAGACCCGA GATGTCATCA CATGTGAGCC ACAGGGCTCC 
CTTATTGACA CCAAGGGCAA CAAGATTGCC GGCTTTGATT CCATCTTCAA GAAAGAGGGT 
CTACAGGTTT CCACCAAACA AAAGATCTCT CCCTGGGATC TTTTTGAGGG GCTGAAGCCG 
TCTGCACCAC TCTCTTGGGG CTGGTTTGGA ACAGTCCGGG TCGACAGGCG AGTGGCCCGA 
GGCGAGGAGC AGCAGCGGTT GCTGCTCTAC CACACACACC TGCGGCCCCG GCCCCGTGCC 
TATTACCTGG AGCCACTGCC ACTGCCACCA GAGGATGAGG AGCCCCCCGC TCCCACCCCG 
CTAGAGCCTG AGAAAAAGGC TCCAGAGCCC CCCAAAACTG ACAAGCCTGG GGCTGCTCCA 
CCTAGTACTG AGGAACGCAA GAAGAAGTCC ACGAAGGGCA AGAAACGCAG CCAGCCTGCT 
GCCAAGACAG AGGACTATGG AATGGGCCCA GGGCGGAGTG GCCCCTATGG TGTGACCGTG 
CCTCCGGACC TCCTGCACCA CGCCAACCCT GGTTCCATAT CCCACCTTAG CTACAGGCAG 
GGTTCCATAG GCCTGTACAC CCAGAACCAG CCACTACCTG CAGGTGGCCC TCGTGTGGAC 
CCATACCGCC CTATGCGGTT ACCAATGCAG AAGCTGCCGA CCCGACCACC TTACCCTGGA 
GTGCTACCCA CAGCCGTGAC TGGCGTCATG GGACTGGAAC CCTCTTCCTA CAAGACCTCT 
GTGTACCGAC AGCAGCAGCC TGCGGTGCCC CAAGGACAGC GCCTTCGCCA ACAGCTCCAG 
GCAAAGATAC AGAGTCAGGG GATGTTGGGA CAGTCATCTG TCCATCAGAT GACTCCCAGC 
TCTTCCTACG GTTTGCAGAC CTCCCAGGGC TATACTCCTT ATGTTTCTCA TGTGGGATTG 
CAGCAACACA CAGGCCCCGC AGGTACCATG GTGCCCCCCA GCTACTCCAG CCAGCCTTAT 
CAGAGCACCC ACCCTTCTAC CAATCCTACT CTTGTAGATC CTACTCGCCA CCTGCAACAG 
CGGCCCAGTG GCTATGTGCA CCAGCAGGCC CCAACCTACG GACATGGGCT GACCTCCAGT 
CAGAGGTTTT CACACCAGAC ACTGCAGCAA ACACCCATGA TGGGTACCAT GACTCCACTG 
GGCGCCCAGG GCGTCCAGGC CGGCGTCCGA TCAGCTTCCA TCTTGCCTGA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGCAGCA ATACCACATC CGGCAGCAGC AGCAGCAGCA GATCCTGCGG 
GTAAGGCCCT GGGATTTCCT CTGGGATCTG GGAGCCCAAG AAGGAGGGGA GGCACAAGCT 
CTTCCAGGTT GTTATGAAGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633672.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489440.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X7, mRNA.

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CloneID	OHb71703
Clone ID Related Accession (Same CDS sequence)	XM_023633672.1
Accession Version	XM_023633672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGGCAAAGA TACAGAGTCA GGGGATGTTG GGACAGTCAT CTGTCCATCA GATGACTCCC 
AGCTCTTCCT ACGGTTTGCA GACCTCCCAG GGCTATACTC CTTATGTTTC TCATGTGGGA 
TTGCAGCAAC ACACAGGCCC CGCAGATCCT ACTCGCCACC TGCAACAGCG GCCCAGTGGC 
TATGTGCACC AGCAGGCCCC AACCTACGGA CATGGGCTGA CCTCCAGTCA GAGGTTTTCA 
CACCAGACAC TGCAGCAAAC ACCCATGATG GGTACCATGA CTCCACTGGG CGCCCAGGGC 
GTCCAGGCCG GCGTCCGATC AGCTTCCATC TTGCCTGAGC AGCAGCAACA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAGCAGCAAT ACCACATCCG GCAGCAGCAG CAGCAGCAGA TCCTGCGGGT AAGGCCCTGG 
GATTTCCTCT GGGATCTGGG AGCCCAAGAA GGAGGGGAGG CACAAGCTCT TCCAGGTTGT 
TATGAAGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489440.1
CDS	56..6367
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGTPSPDKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPADPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633672.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633672.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA CTCCTTCCCC AGACAAGCCT 
ACTGTAGGAA TCCGCTCCTC CTGTGACCGC CACCTGCTGG CTGCCTCCCA GAACCGCATC 
GTGGATGGAG CTGTGTTTGC TGTTCTCAAG GCTGTGTTTG TACTTGGCGA TGCGGAACTG 
AAGGGTTCAG GCTTCACTGT GACAGGAGGA ACAGAAGAAC TTCCAGAGGA GGAGGGGGGA 
GGTGGCAGTG GCGGTCGGAG GCAGGGTGGC CGCAACATCT CTGTGGAGAC AGCCAGTCTG 
GATGTCTATG CCAAGTACGT GCTGCGCAGC ATCTGCCAAC AGGAATGGGT AGGAGAACGT 
TGCCTTAAAT CGCTGTGTGA GGACAGCAAT GACCTACAAG ACCCAGTGTT GAGTAGCGCC 
CAGGCCCAGC GCCTCATGCA GCTCATCTGC TACCCACATC GGCTGCTGGA CAATGAGGAT 
GGGGAAAATC CCCAGCGACA ACGCATTAAG CGTATTCTCC AGAACTTGGA CCAGTGGACC 
ATGCGTCAGT CTTCCCTGGA GCTGCAGCTC ATGATCAAGC AGACCCCTAA CAATGAGATG 
AACTCCCTCT TAGAGAACAT TGCCAAGGCC ACAATCGAGG TGTTCCAACA GTCAGCAGAG 
ACAGGGTCAT CTTCTGGAAA CACTGCAAGC AACATGCCCA GCAGCAGCAA GACCAAGCCT 
GTGCTCAGCT CTCTGGAGCG CTCTGGTGTA TGGCTGGTGG CCCCCCTCAT TGCTAAACTG 
CCCACCTCAG TCCAGGGGCA TGTGTTAAAG GCTGCTGGGG AGGAATTGGA GAAGGGCCAG 
CACCTGGGTT CCTCTTCCCG CAAAGAACGT GATCGACAAA AGCAAAAGAG TATGTCCCTG 
TTGAGCCAGC AGCCTTTCTT GTCCCTGGTG CTGACGTGTC TGAAAGGGCA GGATGAGCAG 
CGTGAGGGAC TCCTTACCTC CCTTTACAGC CAAGTGCACC AGATTGTGAA TAATTGGCGA 
GATGACCAGT ACTTAGATGA TTGCAAACCA AAGCAGCTAA TGCATGAGGC ACTCAAACTG 
CGGCTCAACC TGGTGGGGGG CATGTTTGAC ACGGTGCAGC GCAGCACCCA ACAGACCACA 
GAGTGGGCTG TGCTCCTCCT GGAGATCATC ATCAGCGGCA CTGTCGACAT GCAGTCCAAC 
AACGAGCTCT TCACCACTGT ATTGGACATG CTGAGCGTGC TCATCAATGG GACCCTGGCT 
GCGGACATGT CCAGCATCTC TCAAGGCAGC ATGGAGGAAA ACAAACGCGC CTACATGAAC 
CTGGTGAAGA AGCTGCGGAA AGAGTTGGGG GAGCGCCAAT CAGACAGTCT GGAAAAAGTT 
CGCCAGCTGC TGCCACTGCC CAAGCAGACC CGAGATGTCA TCACATGTGA GCCACAGGGC 
TCCCTTATTG ACACCAAGGG CAACAAGATT GCCGGCTTTG ATTCCATCTT CAAGAAAGAG 
GGTCTACAGG TTTCCACCAA ACAAAAGATC TCTCCCTGGG ATCTTTTTGA GGGGCTGAAG 
CCGTCTGCAC CACTCTCTTG GGGCTGGTTT GGAACAGTCC GGGTCGACAG GCGAGTGGCC 
CGAGGCGAGG AGCAGCAGCG GTTGCTGCTC TACCACACAC ACCTGCGGCC CCGGCCCCGT 
GCCTATTACC TGGAGCCACT GCCACTGCCA CCAGAGGATG AGGAGCCCCC CGCTCCCACC 
CCGCTAGAGC CTGAGAAAAA GGCTCCAGAG CCCCCCAAAA CTGACAAGCC TGGGGCTGCT 
CCACCTAGTA CTGAGGAACG CAAGAAGAAG TCCACGAAGG GCAAGAAACG CAGCCAGCCT 
GCTGCCAAGA CAGAGGACTA TGGAATGGGC CCAGGGCGGA GTGGCCCCTA TGGTGTGACC 
GTGCCTCCGG ACCTCCTGCA CCACGCCAAC CCTGGTTCCA TATCCCACCT TAGCTACAGG 
CAGGGTTCCA TAGGCCTGTA CACCCAGAAC CAGCCACTAC CTGCAGGTGG CCCTCGTGTG 
GACCCATACC GCCCTATGCG GTTACCAATG CAGAAGCTGC CGACCCGACC ACCTTACCCT 
GGAGTGCTAC CCACAGCCGT GACTGGCGTC ATGGGACTGG AACCCTCTTC CTACAAGACC 
TCTGTGTACC GACAGCAGCA GCCTGCGGTG CCCCAAGGAC AGCGCCTTCG CCAACAGCTC 
CAGGCAAAGA TACAGAGTCA GGGGATGTTG GGACAGTCAT CTGTCCATCA GATGACTCCC 
AGCTCTTCCT ACGGTTTGCA GACCTCCCAG GGCTATACTC CTTATGTTTC TCATGTGGGA 
TTGCAGCAAC ACACAGGCCC CGCAGATCCT ACTCGCCACC TGCAACAGCG GCCCAGTGGC 
TATGTGCACC AGCAGGCCCC AACCTACGGA CATGGGCTGA CCTCCAGTCA GAGGTTTTCA 
CACCAGACAC TGCAGCAAAC ACCCATGATG GGTACCATGA CTCCACTGGG CGCCCAGGGC 
GTCCAGGCCG GCGTCCGATC AGCTTCCATC TTGCCTGAGC AGCAGCAACA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAGCAGCAAT ACCACATCCG GCAGCAGCAG CAGCAGCAGA TCCTGCGGGT AAGGCCCTGG 
GATTTCCTCT GGGATCTGGG AGCCCAAGAA GGAGGGGAGG CACAAGCTCT TCCAGGTTGT 
TATGAAGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633673.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489441.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X8, mRNA.

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CloneID	OHb71704
Clone ID Related Accession (Same CDS sequence)	XM_023633673.1
Accession Version	XM_023633673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA ACAAGCCTAC TGTAGGAATC 
CGCTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGAGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTTGGCGATG CGGAACTGAA GGGTTCAGGC 
TTCACTGTGA CAGGAGGAAC AGAAGAACTT CCAGAGGAGG AGGGGGGAGG TGGCAGTGGC 
GGTCGGAGGC AGGGTGGCCG CAACATCTCT GTGGAGACAG CCAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGCAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAATCG 
CTGTGTGAGG ACAGCAATGA CCTACAAGAC CCAGTGTTGA GTAGCGCCCA GGCCCAGCGC 
CTCATGCAGC TCATCTGCTA CCCACATCGG CTGCTGGACA ATGAGGATGG GGAAAATCCC 
CAGCGACAAC GCATTAAGCG TATTCTCCAG AACTTGGACC AGTGGACCAT GCGTCAGTCT 
TCCCTGGAGC TGCAGCTCAT GATCAAGCAG ACCCCTAACA ATGAGATGAA CTCCCTCTTA 
GAGAACATTG CCAAGGCCAC AATCGAGGTG TTCCAACAGT CAGCAGAGAC AGGGTCATCT 
TCTGGAAACA CTGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAGCCTGT GCTCAGCTCT 
CTGGAGCGCT CTGGTGTATG GCTGGTGGCC CCCCTCATTG CTAAACTGCC CACCTCAGTC 
CAGGGGCATG TGTTAAAGGC TGCTGGGGAG GAATTGGAGA AGGGCCAGCA CCTGGGTTCC 
TCTTCCCGCA AAGAACGTGA TCGACAAAAG CAAAAGAGTA TGTCCCTGTT GAGCCAGCAG 
CCTTTCTTGT CCCTGGTGCT GACGTGTCTG AAAGGGCAGG ATGAGCAGCG TGAGGGACTC 
CTTACCTCCC TTTACAGCCA AGTGCACCAG ATTGTGAATA ATTGGCGAGA TGACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC GGTGCAGCGC AGCACCCAAC AGACCACAGA GTGGGCTGTG 
CTCCTCCTGG AGATCATCAT CAGCGGCACT GTCGACATGC AGTCCAACAA CGAGCTCTTC 
ACCACTGTAT TGGACATGCT GAGCGTGCTC ATCAATGGGA CCCTGGCTGC GGACATGTCC 
AGCATCTCTC AAGGCAGCAT GGAGGAAAAC AAACGCGCCT ACATGAACCT GGTGAAGAAG 
CTGCGGAAAG AGTTGGGGGA GCGCCAATCA GACAGTCTGG AAAAAGTTCG CCAGCTGCTG 
CCACTGCCCA AGCAGACCCG AGATGTCATC ACATGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC CGGCTTTGAT TCCATCTTCA AGAAAGAGGG TCTACAGGTT 
TCCACCAAAC AAAAGATCTC TCCCTGGGAT CTTTTTGAGG GGCTGAAGCC GTCTGCACCA 
CTCTCTTGGG GCTGGTTTGG AACAGTCCGG GTCGACAGGC GAGTGGCCCG AGGCGAGGAG 
CAGCAGCGGT TGCTGCTCTA CCACACACAC CTGCGGCCCC GGCCCCGTGC CTATTACCTG 
GAGCCACTGC CACTGCCACC AGAGGATGAG GAGCCCCCCG CTCCCACCCC GCTAGAGCCT 
GAGAAAAAGG CTCCAGAGCC CCCCAAAACT GACAAGCCTG GGGCTGCTCC ACCTAGTACT 
GAGGAACGCA AGAAGAAGTC CACGAAGGGC AAGAAACGCA GCCAGCCTGC TGCCAAGACA 
GAGGACTATG GAATGGGCCC AGGGCGGAGT GGCCCCTATG GTGTGACCGT GCCTCCGGAC 
CTCCTGCACC ACGCCAACCC TGGTTCCATA TCCCACCTTA GCTACAGGCA GGGTTCCATA 
GGCCTGTACA CCCAGAACCA GCCACTACCT GCAGGTGGCC CTCGTGTGGA CCCATACCGC 
CCTATGCGGT TACCAATGCA GAAGCTGCCG ACCCGACCAC CTTACCCTGG AGTGCTACCC 
ACAGCCGTGA CTGGCGTCAT GGGACTGGAA CCCTCTTCCT ACAAGACCTC TGTGTACCGA 
CAGCAGCAGC CTGCGGTGCC CCAAGGACAG CGCCTTCGCC AACAGCTCCA GGCAAAGATA 
CAGAGTCAGG GGATGTTGGG ACAGTCATCT GTCCATCAGA TGACTCCCAG CTCTTCCTAC 
GGTTTGCAGA CCTCCCAGGG CTATACTCCT TATGTTTCTC ATGTGGGATT GCAGCAACAC 
ACAGGCCCCG CAGATCCTAC TCGCCACCTG CAACAGCGGC CCAGTGGCTA TGTGCACCAG 
CAGGCCCCAA CCTACGGACA TGGGCTGACC TCCAGTCAGA GGTTTTCACA CCAGACACTG 
CAGCAAACAC CCATGATGGG TACCATGACT CCACTGGGCG CCCAGGGCGT CCAGGCCGGC 
GTCCGATCAG CTTCCATCTT GCCTGAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAACA GCAGCAATAC 
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGGTAA GGCCCTGGGA TTTCCTCTGG 
GATCTGGGAG CCCAAGAAGG AGGGGAGGCA CAAGCTCTTC CAGGTTGTTA TGAAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489441.1
CDS	55..6354
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPADPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRVRPWDFLWDLGAQEGGEAQALPGCYEG

Target ORF information:

RefSeq Version	XM_023633673.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633673.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA ACAAGCCTAC TGTAGGAATC 
CGCTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGAGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTTGGCGATG CGGAACTGAA GGGTTCAGGC 
TTCACTGTGA CAGGAGGAAC AGAAGAACTT CCAGAGGAGG AGGGGGGAGG TGGCAGTGGC 
GGTCGGAGGC AGGGTGGCCG CAACATCTCT GTGGAGACAG CCAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGCAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAATCG 
CTGTGTGAGG ACAGCAATGA CCTACAAGAC CCAGTGTTGA GTAGCGCCCA GGCCCAGCGC 
CTCATGCAGC TCATCTGCTA CCCACATCGG CTGCTGGACA ATGAGGATGG GGAAAATCCC 
CAGCGACAAC GCATTAAGCG TATTCTCCAG AACTTGGACC AGTGGACCAT GCGTCAGTCT 
TCCCTGGAGC TGCAGCTCAT GATCAAGCAG ACCCCTAACA ATGAGATGAA CTCCCTCTTA 
GAGAACATTG CCAAGGCCAC AATCGAGGTG TTCCAACAGT CAGCAGAGAC AGGGTCATCT 
TCTGGAAACA CTGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAGCCTGT GCTCAGCTCT 
CTGGAGCGCT CTGGTGTATG GCTGGTGGCC CCCCTCATTG CTAAACTGCC CACCTCAGTC 
CAGGGGCATG TGTTAAAGGC TGCTGGGGAG GAATTGGAGA AGGGCCAGCA CCTGGGTTCC 
TCTTCCCGCA AAGAACGTGA TCGACAAAAG CAAAAGAGTA TGTCCCTGTT GAGCCAGCAG 
CCTTTCTTGT CCCTGGTGCT GACGTGTCTG AAAGGGCAGG ATGAGCAGCG TGAGGGACTC 
CTTACCTCCC TTTACAGCCA AGTGCACCAG ATTGTGAATA ATTGGCGAGA TGACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC GGTGCAGCGC AGCACCCAAC AGACCACAGA GTGGGCTGTG 
CTCCTCCTGG AGATCATCAT CAGCGGCACT GTCGACATGC AGTCCAACAA CGAGCTCTTC 
ACCACTGTAT TGGACATGCT GAGCGTGCTC ATCAATGGGA CCCTGGCTGC GGACATGTCC 
AGCATCTCTC AAGGCAGCAT GGAGGAAAAC AAACGCGCCT ACATGAACCT GGTGAAGAAG 
CTGCGGAAAG AGTTGGGGGA GCGCCAATCA GACAGTCTGG AAAAAGTTCG CCAGCTGCTG 
CCACTGCCCA AGCAGACCCG AGATGTCATC ACATGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC CGGCTTTGAT TCCATCTTCA AGAAAGAGGG TCTACAGGTT 
TCCACCAAAC AAAAGATCTC TCCCTGGGAT CTTTTTGAGG GGCTGAAGCC GTCTGCACCA 
CTCTCTTGGG GCTGGTTTGG AACAGTCCGG GTCGACAGGC GAGTGGCCCG AGGCGAGGAG 
CAGCAGCGGT TGCTGCTCTA CCACACACAC CTGCGGCCCC GGCCCCGTGC CTATTACCTG 
GAGCCACTGC CACTGCCACC AGAGGATGAG GAGCCCCCCG CTCCCACCCC GCTAGAGCCT 
GAGAAAAAGG CTCCAGAGCC CCCCAAAACT GACAAGCCTG GGGCTGCTCC ACCTAGTACT 
GAGGAACGCA AGAAGAAGTC CACGAAGGGC AAGAAACGCA GCCAGCCTGC TGCCAAGACA 
GAGGACTATG GAATGGGCCC AGGGCGGAGT GGCCCCTATG GTGTGACCGT GCCTCCGGAC 
CTCCTGCACC ACGCCAACCC TGGTTCCATA TCCCACCTTA GCTACAGGCA GGGTTCCATA 
GGCCTGTACA CCCAGAACCA GCCACTACCT GCAGGTGGCC CTCGTGTGGA CCCATACCGC 
CCTATGCGGT TACCAATGCA GAAGCTGCCG ACCCGACCAC CTTACCCTGG AGTGCTACCC 
ACAGCCGTGA CTGGCGTCAT GGGACTGGAA CCCTCTTCCT ACAAGACCTC TGTGTACCGA 
CAGCAGCAGC CTGCGGTGCC CCAAGGACAG CGCCTTCGCC AACAGCTCCA GGCAAAGATA 
CAGAGTCAGG GGATGTTGGG ACAGTCATCT GTCCATCAGA TGACTCCCAG CTCTTCCTAC 
GGTTTGCAGA CCTCCCAGGG CTATACTCCT TATGTTTCTC ATGTGGGATT GCAGCAACAC 
ACAGGCCCCG CAGATCCTAC TCGCCACCTG CAACAGCGGC CCAGTGGCTA TGTGCACCAG 
CAGGCCCCAA CCTACGGACA TGGGCTGACC TCCAGTCAGA GGTTTTCACA CCAGACACTG 
CAGCAAACAC CCATGATGGG TACCATGACT CCACTGGGCG CCCAGGGCGT CCAGGCCGGC 
GTCCGATCAG CTTCCATCTT GCCTGAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAACA GCAGCAATAC 
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGGTAA GGCCCTGGGA TTTCCTCTGG 
GATCTGGGAG CCCAAGAAGG AGGGGAGGCA CAAGCTCTTC CAGGTTGTTA TGAAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_023633675.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489443.1 Equus caballus mediator complex subunit 12 (MED12), transcript variant X10, mRNA.

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CloneID	OHb71706
Clone ID Related Accession (Same CDS sequence)	XM_023633675.1
Accession Version	XM_023633675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA ACAAGCCTAC TGTAGGAATC 
CGCTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGAGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTTGGCGATG CGGAACTGAA GGGTTCAGGC 
TTCACTGTGA CAGGAGGAAC AGAAGAACTT CCAGAGGAGG AGGGGGGAGG TGGCAGTGGC 
GGTCGGAGGC AGGGTGGCCG CAACATCTCT GTGGAGACAG CCAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGCAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAATCG 
CTGTGTGAGG ACAGCAATGA CCTACAAGAC CCAGTGTTGA GTAGCGCCCA GGCCCAGCGC 
CTCATGCAGC TCATCTGCTA CCCACATCGG CTGCTGGACA ATGAGGATGG GGAAAATCCC 
CAGCGACAAC GCATTAAGCG TATTCTCCAG AACTTGGACC AGTGGACCAT GCGTCAGTCT 
TCCCTGGAGC TGCAGCTCAT GATCAAGCAG ACCCCTAACA ATGAGATGAA CTCCCTCTTA 
GAGAACATTG CCAAGGCCAC AATCGAGGTG TTCCAACAGT CAGCAGAGAC AGGGTCATCT 
TCTGGAAACA CTGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAGCCTGT GCTCAGCTCT 
CTGGAGCGCT CTGGTGTATG GCTGGTGGCC CCCCTCATTG CTAAACTGCC CACCTCAGTC 
CAGGGGCATG TGTTAAAGGC TGCTGGGGAG GAATTGGAGA AGGGCCAGCA CCTGGGTTCC 
TCTTCCCGCA AAGAACGTGA TCGACAAAAG CAAAAGAGTA TGTCCCTGTT GAGCCAGCAG 
CCTTTCTTGT CCCTGGTGCT GACGTGTCTG AAAGGGCAGG ATGAGCAGCG TGAGGGACTC 
CTTACCTCCC TTTACAGCCA AGTGCACCAG ATTGTGAATA ATTGGCGAGA TGACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC GGTGCAGCGC AGCACCCAAC AGACCACAGA GTGGGCTGTG 
CTCCTCCTGG AGATCATCAT CAGCGGCACT GTCGACATGC AGTCCAACAA CGAGCTCTTC 
ACCACTGTAT TGGACATGCT GAGCGTGCTC ATCAATGGGA CCCTGGCTGC GGACATGTCC 
AGCATCTCTC AAGGCAGCAT GGAGGAAAAC AAACGCGCCT ACATGAACCT GGTGAAGAAG 
CTGCGGAAAG AGTTGGGGGA GCGCCAATCA GACAGTCTGG AAAAAGTTCG CCAGCTGCTG 
CCACTGCCCA AGCAGACCCG AGATGTCATC ACATGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC CGGCTTTGAT TCCATCTTCA AGAAAGAGGG TCTACAGGTT 
TCCACCAAAC AAAAGATCTC TCCCTGGGAT CTTTTTGAGG GGCTGAAGCC GTCTGCACCA 
CTCTCTTGGG GCTGGTTTGG AACAGTCCGG GTCGACAGGC GAGTGGCCCG AGGCGAGGAG 
CAGCAGCGGT TGCTGCTCTA CCACACACAC CTGCGGCCCC GGCCCCGTGC CTATTACCTG 
GAGCCACTGC CACTGCCACC AGAGGATGAG GAGCCCCCCG CTCCCACCCC GCTAGAGCCT 
GAGAAAAAGG CTCCAGAGCC CCCCAAAACT GACAAGCCTG GGGCTGCTCC ACCTAGTACT 
GAGGAACGCA AGAAGAAGTC CACGAAGGGC AAGAAACGCA GCCAGCCTGC TGCCAAGACA 
GAGGACTATG GAATGGGCCC AGGGCGGAGT GGCCCCTATG GTGTGACCGT GCCTCCGGAC 
CTCCTGCACC ACGCCAACCC TGGTTCCATA TCCCACCTTA GCTACAGGCA GGGTTCCATA 
GGCCTGTACA CCCAGAACCA GCCACTACCT GCAGGTGGCC CTCGTGTGGA CCCATACCGC 
CCTATGCGGT TACCAATGCA GAAGCTGCCG ACCCGACCAC CTTACCCTGG AGTGCTACCC 
ACAGCCGTGA CTGGCGTCAT GGGACTGGAA CCCTCTTCCT ACAAGACCTC TGTGTACCGA 
CAGCAGCAGC CTGCGGTGCC CCAAGGACAG CGCCTTCGCC AACAGCTCCA GCAGAGTCAG 
GGGATGTTGG GACAGTCATC TGTCCATCAG ATGACTCCCA GCTCTTCCTA CGGTTTGCAG 
ACCTCCCAGG GCTATACTCC TTATGTTTCT CATGTGGGAT TGCAGCAACA CACAGGCCCC 
GCAGGTACCA TGGTGCCCCC CAGCTACTCC AGCCAGCCTT ATCAGAGCAC CCACCCTTCT 
ACCAATCCTA CTCTTGTAGA TCCTACTCGC CACCTGCAAC AGCGGCCCAG TGGCTATGTG 
CACCAGCAGG CCCCAACCTA CGGACATGGG CTGACCTCCA GTCAGAGGTT TTCACACCAG 
ACACTGCAGC AAACACCCAT GATGGGTACC ATGACTCCAC TGGGCGCCCA GGGCGTCCAG 
GCCGGCGTCC GATCAGCTTC CATCTTGCCT GAGCAGCAGC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGCAG 
CAATACCACA TCCGGCAGCA GCAGCAGCAG CAGATCCTGC GGCAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAACA GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAACAGCAA 
GCACACCAGC AGCAGCAGCA GCAGGCGGCT CCTCCCCAAC CCCAGCCCCA GTCCCAGCCC 
CAGTTCCAGC GCCAGGGGCT TCAGCAGACA CAGCAACAGC AACAGACAGC AGCTTTGGTC 
CGGCAGCTCC AACAACAGCT CTCCAATACC CAGCCACAGC CCAGTACCAA CCTATTTGGA 
CGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489443.1
CDS	55..6603
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAITETKVKKRHVIDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVMSAQSTGTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGNTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTPLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPAAKTEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQGSIGLYTQNQPLPAGGPRVDPYRPMRLPMQKLPTRPPYPGVLPTAVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSSQRFSHQTLQQTPMMGTMTPLGAQGVQAGVRSASILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQAHQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNLFGRF

Target ORF information:

RefSeq Version	XM_023633675.1
Organism	Equus caballus(horse)
Definition	Equus caballus mediator complex subunit 12 (MED12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633675.1

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCCTGAAT 
GTAAAACAAG GCTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTCCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACGGC GCGATCCCAG AGTGCCATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AGCCACTCAC ACAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACCT GTGCCTACTA TGCAGCAATC ACTGAGACCA AGGTTAAGAA GAGACATGTC 
ATTGACCCCT TCATGGAATG GACTCAGATC ATCACCAAGT ACTTATGGGA GCAGCTGCAA 
AAGATGGCTG AATACTACCG GCCAGGGCCT GCAGGAAGCG GGGGCTGTGG TTCCACTATA 
GGGCCCTTGC CCCATGATGT AGAAGTGGCA ATCCGGCAGT GGGACTACAA TGAGAAGCTG 
GCCATGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC CTGGGTACTT 
GAGTGTTTTG AGAAAATCCG CCCTGGAGAG GATGAATTGC TTAAACTGCT GTTGCCCCTG 
CTGCTTCGAT ACTCTGGGGA ATTCGTTCAG TCTGCATACC TCTCTCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGGCTGGC CCTGCAGCTG GATGGCGTGA GCAGTCACTC GTCTCATGTG 
ATGTCTGCTC AGTCGACAGG CACACTGCCC ACCACCCCTG CTCCTCAGCC CCCGACTAGC 
AGCACACCCT CTACACCCTT TAGTGACCTA CTTATGTGCC CTCAGCACCG GCCCCTAGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTCCTGTGTT GTCCTAGTGC CCTGGTTTGG 
CACTACTCAC TGACTGATAG CCGAATTAAG ACTGGCTCAC CACTTGACCA CCTGCCTATT 
GCCCCCTCCA ACCTGCCCAT GCCAGAGGGC AACAGTGCCT TCACTCAGCA GGTCCGTGCA 
AAGTTGCGGG AGATTGAGCA GCAGATCAAG GAGCGAGGAC AGGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGCTTCACCA TTGGACGGGT GCTCCATACT 
TTGGAAGTGC TGGACAGCCA TAGTTTTGAG CGCTCTGACT TCAGCAACTC TCTTGACTCC 
TTGTGTAACC GAATCTTTGG ATTGGGGCCT AGCAAGGATG GGCATGAGAT CTCCTCAGAT 
GATGATGCTG TGGTATCATT ACTGTGTGAA TGGGCTGTCA GCTGCAAGCG TTCTGGTCGG 
CATCGTGCTA TGGTGGTAGC CAAGCTGCTG GAGAAGAGAC AGGCAGAGAT TGAGGCTGAG 
CGTTGTGGAG AATCAGAAGC CGCAGATGAA AAGGGTTCCA TCGCCTCTGG CTCCCTTTCT 
GCTCCTAGTG CTCCCATTTT CCAGGATGTT CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACGG ACCCCCGAAG TGAGAGTGAG CGGGTGGAGT TCTTTAACTT GGTACTGCTG 
TTCTGTGAAC TCATTCGACA TGATGTTTTC TCCCACAACA TGTATACTTG CACCCTCATC 
TCCCGAGGGG ACCTTGCCTT TGGAGCCCCT GGTCCCCGGC CTCCCTCTCC CTTTGATGAC 
CCTGCTGATG ACCCAGAGCG CAAGGAGGCT GAGGGCAGCA GCAGTAGCAA GCTGGAGGAT 
CCGGGGCTCT CGGAGTCTAT GGACATAGAC CCTAGTTCCA GTGTACTGTT TGAGGACATG 
GAGAAGCCTG ATTTCTCATT GTTCTCTCCT ACTATGCCCT GTGAGGGGAA GGGCAGTCCA 
TCCCCTGAGA AACCAGATGT TGAGAAGGAG GTGAAGCCCC CACCCAAGGA GAAGATAGAA 
GGGACCCTTG GGGTTCTTTA TGACCAGCCA CGGCATGTGC AGTATGCCAC ACACTTTCCC 
ATCCCCCAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGAAAGC AGCGAGATGA TGCCCGACAT GCCATCAAGA AAATAACCAA GGATATCCTG 
AAGGTTCTGA ACCGCAAGGG GACGGCGGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAGGATGGGC AGAAGCGACG GCGCAACCGG 
CCTGAAGCCT TCCCCACTGC TGAAGATATC TTTGCTAAGT TCCAGCATCT TTCACATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAATGTTC TGGAGCAGAT CACGAGTTTT 
GCCCTTGGCA TGTCATACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTCAGCAT CAGTGGCCTC ATCGACTTTG CCATTCAGCT ACTGAATGAA 
CTGAGTGTAG TTGAGGCTGA GTTGCTTCTC AAATCCTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCCTGTGCAT TGTGGCTGTC CTGCGGCACT ATCATGCCTG CCTCATCCTC 
AACCAGGACC AGATGGCACA GGTCTTTGAG GGGCTGTGTG GCGTAGTGAA GCATGGGATG 
AACCGGTCAG ATGGCTCCTC TGCAGAACGC TGTATCCTTG CTTATCTCTA TGATCTGTAC 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGCGACTT CTGCTCCAAG 
GTGAAGAACA CCATCTACTG CAACGTGGAG CCGTCAGAAT CCAATATGCG CTGGGCACCT 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCAGCTCACA CCTTCACCTA CACGGGGCTA 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTACAGCT TTGTCTGCAA TGCCCTTATG 
CACGTCTGTG TGGGGCACCA TGATCCCGAT AGGGTGAATG ACATCGCAAT CCTGTGTGCA 
GAGCTGACCG GCTATTGCAA GTCACTGAGT GCGGAATGGC TAGGAGTCCT TAAAGCCTTG 
TGCTGCTCCT CTAACAATGG CACTTGTGGT TTCAACGACC TCCTCTGCAA TGTAGATGTC 
AGTGACCTGT CTTTTCATGA TTCCCTGGCT ACTTTTGTTG CCATCCTCAT CGCTCGGCAG 
TGTTTGCTCC TAGAGGATCT GATTCGCTGT GCTGCCATCC CTTCACTCCT TAATGCTGCT 
TGCAGTGAGC AGGATTCTGA GCCCGGGGCC CGACTTACCT GCCGCATCCT CCTTCACCTT 
TTCAAGACAC CTCAACTCAA TCCTTGCCAG TCGGATGGAA ACAAGCCTAC TGTAGGAATC 
CGCTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGAGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTTGGCGATG CGGAACTGAA GGGTTCAGGC 
TTCACTGTGA CAGGAGGAAC AGAAGAACTT CCAGAGGAGG AGGGGGGAGG TGGCAGTGGC 
GGTCGGAGGC AGGGTGGCCG CAACATCTCT GTGGAGACAG CCAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGCAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAATCG 
CTGTGTGAGG ACAGCAATGA CCTACAAGAC CCAGTGTTGA GTAGCGCCCA GGCCCAGCGC 
CTCATGCAGC TCATCTGCTA CCCACATCGG CTGCTGGACA ATGAGGATGG GGAAAATCCC 
CAGCGACAAC GCATTAAGCG TATTCTCCAG AACTTGGACC AGTGGACCAT GCGTCAGTCT 
TCCCTGGAGC TGCAGCTCAT GATCAAGCAG ACCCCTAACA ATGAGATGAA CTCCCTCTTA 
GAGAACATTG CCAAGGCCAC AATCGAGGTG TTCCAACAGT CAGCAGAGAC AGGGTCATCT 
TCTGGAAACA CTGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAGCCTGT GCTCAGCTCT 
CTGGAGCGCT CTGGTGTATG GCTGGTGGCC CCCCTCATTG CTAAACTGCC CACCTCAGTC 
CAGGGGCATG TGTTAAAGGC TGCTGGGGAG GAATTGGAGA AGGGCCAGCA CCTGGGTTCC 
TCTTCCCGCA AAGAACGTGA TCGACAAAAG CAAAAGAGTA TGTCCCTGTT GAGCCAGCAG 
CCTTTCTTGT CCCTGGTGCT GACGTGTCTG AAAGGGCAGG ATGAGCAGCG TGAGGGACTC 
CTTACCTCCC TTTACAGCCA AGTGCACCAG ATTGTGAATA ATTGGCGAGA TGACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC GGTGCAGCGC AGCACCCAAC AGACCACAGA GTGGGCTGTG 
CTCCTCCTGG AGATCATCAT CAGCGGCACT GTCGACATGC AGTCCAACAA CGAGCTCTTC 
ACCACTGTAT TGGACATGCT GAGCGTGCTC ATCAATGGGA CCCTGGCTGC GGACATGTCC 
AGCATCTCTC AAGGCAGCAT GGAGGAAAAC AAACGCGCCT ACATGAACCT GGTGAAGAAG 
CTGCGGAAAG AGTTGGGGGA GCGCCAATCA GACAGTCTGG AAAAAGTTCG CCAGCTGCTG 
CCACTGCCCA AGCAGACCCG AGATGTCATC ACATGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC CGGCTTTGAT TCCATCTTCA AGAAAGAGGG TCTACAGGTT 
TCCACCAAAC AAAAGATCTC TCCCTGGGAT CTTTTTGAGG GGCTGAAGCC GTCTGCACCA 
CTCTCTTGGG GCTGGTTTGG AACAGTCCGG GTCGACAGGC GAGTGGCCCG AGGCGAGGAG 
CAGCAGCGGT TGCTGCTCTA CCACACACAC CTGCGGCCCC GGCCCCGTGC CTATTACCTG 
GAGCCACTGC CACTGCCACC AGAGGATGAG GAGCCCCCCG CTCCCACCCC GCTAGAGCCT 
GAGAAAAAGG CTCCAGAGCC CCCCAAAACT GACAAGCCTG GGGCTGCTCC ACCTAGTACT 
GAGGAACGCA AGAAGAAGTC CACGAAGGGC AAGAAACGCA GCCAGCCTGC TGCCAAGACA 
GAGGACTATG GAATGGGCCC AGGGCGGAGT GGCCCCTATG GTGTGACCGT GCCTCCGGAC 
CTCCTGCACC ACGCCAACCC TGGTTCCATA TCCCACCTTA GCTACAGGCA GGGTTCCATA 
GGCCTGTACA CCCAGAACCA GCCACTACCT GCAGGTGGCC CTCGTGTGGA CCCATACCGC 
CCTATGCGGT TACCAATGCA GAAGCTGCCG ACCCGACCAC CTTACCCTGG AGTGCTACCC 
ACAGCCGTGA CTGGCGTCAT GGGACTGGAA CCCTCTTCCT ACAAGACCTC TGTGTACCGA 
CAGCAGCAGC CTGCGGTGCC CCAAGGACAG CGCCTTCGCC AACAGCTCCA GCAGAGTCAG 
GGGATGTTGG GACAGTCATC TGTCCATCAG ATGACTCCCA GCTCTTCCTA CGGTTTGCAG 
ACCTCCCAGG GCTATACTCC TTATGTTTCT CATGTGGGAT TGCAGCAACA CACAGGCCCC 
GCAGGTACCA TGGTGCCCCC CAGCTACTCC AGCCAGCCTT ATCAGAGCAC CCACCCTTCT 
ACCAATCCTA CTCTTGTAGA TCCTACTCGC CACCTGCAAC AGCGGCCCAG TGGCTATGTG 
CACCAGCAGG CCCCAACCTA CGGACATGGG CTGACCTCCA GTCAGAGGTT TTCACACCAG 
ACACTGCAGC AAACACCCAT GATGGGTACC ATGACTCCAC TGGGCGCCCA GGGCGTCCAG 
GCCGGCGTCC GATCAGCTTC CATCTTGCCT GAGCAGCAGC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGCAG 
CAATACCACA TCCGGCAGCA GCAGCAGCAG CAGATCCTGC GGCAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAACA GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAACAGCAA 
GCACACCAGC AGCAGCAGCA GCAGGCGGCT CCTCCCCAAC CCCAGCCCCA GTCCCAGCCC 
CAGTTCCAGC GCCAGGGGCT TCAGCAGACA CAGCAACAGC AACAGACAGC AGCTTTGGTC 
CGGCAGCTCC AACAACAGCT CTCCAATACC CAGCCACAGC CCAGTACCAA CCTATTTGGA 
CGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MED12
Entrez Gene ID	100056909
Full Name	mediator complex subunit 12
General protein information	Preferred Names mediator complex subunit 12 Names mediator of RNA polymerase II transcription subunit 12
Gene Type	protein-coding
Organism	Equus caballus(horse)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MED12 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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