FN1 gene cDNA ORF clone, Equus caballus(horse)

mRNA	Protein	Name
XM_023642284.1	XP_023498052.1	fibronectin isoform X6
XM_023642285.1	XP_023498053.1	fibronectin isoform X7
XM_001489104.5	XP_001489154.4	LOW QUALITY PROTEIN: fibronectin
XM_023642290.1	XP_023498058.1	fibronectin isoform X11
XM_023642283.1	XP_023498051.1	fibronectin isoform X5
XM_023642282.1	XP_023498050.1	fibronectin isoform X4
XM_023642289.1	XP_023498057.1	fibronectin isoform X10
XM_023642291.1	XP_023498059.1	fibronectin isoform X12
XM_001489104.6	XP_001489154.5	fibronectin isoform X1
XM_023642280.1	XP_023498048.1	fibronectin isoform X2
XM_023642281.1	XP_023498049.1	fibronectin isoform X3
XM_023642286.1	XP_023498054.1	fibronectin isoform X8
XM_023642287.1	XP_023498055.1	fibronectin isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb31020	XM_001489104.5 Latest version!	Equus caballus fibronectin 1 (FN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66100	XM_001489104.6 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66101	XM_023642280.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66102	XM_023642281.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66103	XM_023642282.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66104	XM_023642283.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66105	XM_023642284.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66106	XM_023642285.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb66107	XM_023642286.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb66108	XM_023642287.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb66109	XM_023642289.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb66110	XM_023642290.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb66111	XM_023642291.1 Latest version!	Equus caballus fibronectin 1 (FN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

FN1 ( XM_001489104.5 ) cDNA ORF clone, Equus caballus(horse) -> XP_001489154.4 Equus caballus fibronectin 1 (FN1), mRNA.

Price & Availability↑

CloneID	OHb31020
Clone ID Related Accession (Same CDS sequence)	XM_001489104.5
Accession Version	XM_001489104.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1447862400000
Organism	Equus caballus(horse)
Product	LOW QUALITY PROTEIN: fibronectin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867421.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001489104.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGACAACCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAATTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTTA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGATG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA CGGACCAGAG 
ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC 
AATGGAAACG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AGCCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACAACGGC CACCCCCGTA 
AGGCATAGGC CAAGACCATA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC 
CCCAGGGGAG ACGTAGACCA GCACCTCTAC CCTCACGTTC TGGGACTCAA TCCAAATACC 
TCTACAGGAC AAGAGGCTCT CTCTCAGACA ACCATCTCTT GGACCCCATT CCAGGAAAGC 
TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA 
GTTCCTGGAA CCTCTGCTAG TGCCACCCTG ACGGGTCTTA CCAGAGGGGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGAAG AGGCACAAGG TTCGGGAGGA GGTCGTTACT 
GTGGGCAACT CTGTTGACCA AGGCCTAGGC CAACCTACGG CTGACTCGTG CTTCGACCCC 
TACACCGTCT CCCATTACGC CATCGGAGAG GAGTGGGAGC GACTGTCTGA ATCTGGCTTT 
AAACTCTCCT GCCAGTGCTT AGGGTTTGGC AGTGGCCATT TCAGATGCGA TTCGTCTAAG 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAAG CAACATGTTA TGATGACGGG AAGACGTACC ACGTAGGAGA ACAGTGGCAG 
AAGGAGTATC TTGGTGCTAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTCACG AAGGCTCCAC TGGGCACTCC 
TACAACCAGT ATTCACAGAG ATACCAGCAG AGAACCAACA CCAATGTCAA CTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001489154.4
CDS	67..7500
Translation	MLRGPGPGLLLLVVLSLGQPVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTNIDRPRGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_001489104.5
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001489104.5

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGACAACCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAATTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTTA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGATG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA CGGACCAGAG 
ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC 
AATGGAAACG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AGCCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACAACGGC CACCCCCGTA 
AGGCATAGGC CAAGACCATA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC 
CCCAGGGGAG ACGTAGACCA GCACCTCTAC CCTCACGTTC TGGGACTCAA TCCAAATACC 
TCTACAGGAC AAGAGGCTCT CTCTCAGACA ACCATCTCTT GGACCCCATT CCAGGAAAGC 
TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA 
GTTCCTGGAA CCTCTGCTAG TGCCACCCTG ACGGGTCTTA CCAGAGGGGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGAAG AGGCACAAGG TTCGGGAGGA GGTCGTTACT 
GTGGGCAACT CTGTTGACCA AGGCCTAGGC CAACCTACGG CTGACTCGTG CTTCGACCCC 
TACACCGTCT CCCATTACGC CATCGGAGAG GAGTGGGAGC GACTGTCTGA ATCTGGCTTT 
AAACTCTCCT GCCAGTGCTT AGGGTTTGGC AGTGGCCATT TCAGATGCGA TTCGTCTAAG 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAAG CAACATGTTA TGATGACGGG AAGACGTACC ACGTAGGAGA ACAGTGGCAG 
AAGGAGTATC TTGGTGCTAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTCACG AAGGCTCCAC TGGGCACTCC 
TACAACCAGT ATTCACAGAG ATACCAGCAG AGAACCAACA CCAATGTCAA CTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_001489104.6 ) cDNA ORF clone, Equus caballus(horse) -> XP_001489154.5 Equus caballus fibronectin 1 (FN1), transcript variant X1, mRNA.

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CloneID	OHb66100
Clone ID Related Accession (Same CDS sequence)	XM_001489104.6
Accession Version	XM_001489104.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_001489104.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGATG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA CGGACCAGAG 
ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC 
AATGGAAACG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AGCCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACAACGGC CACCCCCGTA 
AGGCATAGGC CAAGACCATA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC 
CCCAGGGGAG ACGTAGACCA GCACCTCTAC CCTCACGTTC TGGGACTCAA TCCAAATACC 
TCTACAGGAC AAGAGGCTCT CTCTCAGACA ACCATCTCTT GGACCCCATT CCAGGAAAGC 
TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA 
GTTCCTGGAA CCTCTGCTAG TGCCACCCTG ACGGGTCTTA CCAGAGGGGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGAAG AGGCACAAGG TTCGGGAGGA GGTCGTTACT 
GTGGGCAACT CTGTTGACCA AGGCCTAGGC CAACCTACGG CTGACTCGTG CTTCGACCCC 
TACACCGTCT CCCATTACGC CATCGGAGAG GAGTGGGAGC GACTGTCTGA ATCTGGCTTT 
AAACTCTCCT GCCAGTGCTT AGGGTTTGGC AGTGGCCATT TCAGATGCGA TTCGTCTAAG 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAAG CAACATGTTA TGATGACGGG AAGACGTACC ACGTAGGAGA ACAGTGGCAG 
AAGGAGTATC TTGGTGCTAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTCACG AAGGCTCCAC TGGGCACTCC 
TACAACCAGT ATTCACAGAG ATACCAGCAG AGAACCAACA CCAATGTCAA CTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001489154.5
CDS	268..7701
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTNIDRPRGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_001489104.6
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001489104.6

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGATG AGCTTCCCCA ACTGGTAACC CTTCCACACC CCAATCTTCA CGGACCAGAG 
ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC 
AATGGAAACG GTATTCAGCT TCCTGGCACT TCTGGTCAGC AGCCCAGTGT TGGGCAACAA 
ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACAACGGC CACCCCCGTA 
AGGCATAGGC CAAGACCATA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC 
CCCAGGGGAG ACGTAGACCA GCACCTCTAC CCTCACGTTC TGGGACTCAA TCCAAATACC 
TCTACAGGAC AAGAGGCTCT CTCTCAGACA ACCATCTCTT GGACCCCATT CCAGGAAAGC 
TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA 
GTTCCTGGAA CCTCTGCTAG TGCCACCCTG ACGGGTCTTA CCAGAGGGGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGAAG AGGCACAAGG TTCGGGAGGA GGTCGTTACT 
GTGGGCAACT CTGTTGACCA AGGCCTAGGC CAACCTACGG CTGACTCGTG CTTCGACCCC 
TACACCGTCT CCCATTACGC CATCGGAGAG GAGTGGGAGC GACTGTCTGA ATCTGGCTTT 
AAACTCTCCT GCCAGTGCTT AGGGTTTGGC AGTGGCCATT TCAGATGCGA TTCGTCTAAG 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAAG CAACATGTTA TGATGACGGG AAGACGTACC ACGTAGGAGA ACAGTGGCAG 
AAGGAGTATC TTGGTGCTAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTCACG AAGGCTCCAC TGGGCACTCC 
TACAACCAGT ATTCACAGAG ATACCAGCAG AGAACCAACA CCAATGTCAA CTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642280.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498048.1 Equus caballus fibronectin 1 (FN1), transcript variant X2, mRNA.

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CloneID	OHb66101
Clone ID Related Accession (Same CDS sequence)	XM_023642280.1
Accession Version	XM_023642280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGTTC AAAAGACCCC TTTCATCACC AACCCTGGGT ATGACAATGG AAACGGTATT 
CAGCTTCCTG GCACTTCTGG TCAGCAGCCC AGTGTTGGGC AACAAATGAT CTTTGAGGAG 
CATGGTTTTA GGCGAACCAC ACCGCCCACA ACGGCCACCC CCGTAAGGCA TAGGCCAAGA 
CCATATCCGC CGAATGTAAA TGAGGAGATC CAAATTGGTC ATGTCCCCAG GGGAGACGTA 
GACCAGCACC TCTACCCTCA CGTTCTGGGA CTCAATCCAA ATACCTCTAC AGGACAAGAG 
GCTCTCTCTC AGACAACCAT CTCTTGGACC CCATTCCAGG AAAGCTCTGA GTATATCATT 
TCATGTCATC CAGTTGGCAT TGATGAAGAA CCCTTACAGT TCCGAGTTCC TGGAACCTCT 
GCTAGTGCCA CCCTGACGGG TCTTACCAGA GGGGCCACCT ACAACATCAT AGTGGAGGCA 
CTGAAAGACC AGAAGAGGCA CAAGGTTCGG GAGGAGGTCG TTACTGTGGG CAACTCTGTT 
GACCAAGGCC TAGGCCAACC TACGGCTGAC TCGTGCTTCG ACCCCTACAC CGTCTCCCAT 
TACGCCATCG GAGAGGAGTG GGAGCGACTG TCTGAATCTG GCTTTAAACT CTCCTGCCAG 
TGCTTAGGGT TTGGCAGTGG CCATTTCAGA TGCGATTCGT CTAAGTGGTG CCATGACAAT 
GGTGTGAACT ACAAGATTGG AGAGAAGTGG GATCGTCAGG GAGAAAATGG CCAGATGATG 
AGCTGCACAT GTCTTGGAAA TGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAAGCAACA 
TGTTATGATG ACGGGAAGAC GTACCACGTA GGAGAACAGT GGCAGAAGGA GTATCTTGGT 
GCTATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC 
AGACCTGGGG CTGAACCCGG TCACGAAGGC TCCACTGGGC ACTCCTACAA CCAGTATTCA 
CAGAGATACC AGCAGAGAAC CAACACCAAT GTCAACTGCC CAATTGAGTG CTTCATGCCT 
TTAGATGTAC AGGCTGACAG AGATGATTCC CGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498048.1
CDS	268..7626
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTNIDRPRGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642280.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642280.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGTTC AAAAGACCCC TTTCATCACC AACCCTGGGT ATGACAATGG AAACGGTATT 
CAGCTTCCTG GCACTTCTGG TCAGCAGCCC AGTGTTGGGC AACAAATGAT CTTTGAGGAG 
CATGGTTTTA GGCGAACCAC ACCGCCCACA ACGGCCACCC CCGTAAGGCA TAGGCCAAGA 
CCATATCCGC CGAATGTAAA TGAGGAGATC CAAATTGGTC ATGTCCCCAG GGGAGACGTA 
GACCAGCACC TCTACCCTCA CGTTCTGGGA CTCAATCCAA ATACCTCTAC AGGACAAGAG 
GCTCTCTCTC AGACAACCAT CTCTTGGACC CCATTCCAGG AAAGCTCTGA GTATATCATT 
TCATGTCATC CAGTTGGCAT TGATGAAGAA CCCTTACAGT TCCGAGTTCC TGGAACCTCT 
GCTAGTGCCA CCCTGACGGG TCTTACCAGA GGGGCCACCT ACAACATCAT AGTGGAGGCA 
CTGAAAGACC AGAAGAGGCA CAAGGTTCGG GAGGAGGTCG TTACTGTGGG CAACTCTGTT 
GACCAAGGCC TAGGCCAACC TACGGCTGAC TCGTGCTTCG ACCCCTACAC CGTCTCCCAT 
TACGCCATCG GAGAGGAGTG GGAGCGACTG TCTGAATCTG GCTTTAAACT CTCCTGCCAG 
TGCTTAGGGT TTGGCAGTGG CCATTTCAGA TGCGATTCGT CTAAGTGGTG CCATGACAAT 
GGTGTGAACT ACAAGATTGG AGAGAAGTGG GATCGTCAGG GAGAAAATGG CCAGATGATG 
AGCTGCACAT GTCTTGGAAA TGGAAAAGGA GAATTCAAGT GTGACCCTCA TGAAGCAACA 
TGTTATGATG ACGGGAAGAC GTACCACGTA GGAGAACAGT GGCAGAAGGA GTATCTTGGT 
GCTATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC 
AGACCTGGGG CTGAACCCGG TCACGAAGGC TCCACTGGGC ACTCCTACAA CCAGTATTCA 
CAGAGATACC AGCAGAGAAC CAACACCAAT GTCAACTGCC CAATTGAGTG CTTCATGCCT 
TTAGATGTAC AGGCTGACAG AGATGATTCC CGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642281.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498049.1 Equus caballus fibronectin 1 (FN1), transcript variant X3, mRNA.

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CloneID	OHb66102
Clone ID Related Accession (Same CDS sequence)	XM_023642281.1
Accession Version	XM_023642281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCACCATTC CTGCACCAAC TGAACTCAAG TTCACTCAGG TTACACCAAC AAGCCTGACC 
GCCCAGTGGA CAGCACCCAA TGTTCAGCTC ACTGGGTACC GCGTGCGGGT GACCCCCAAG 
GAAAAGACTG GACCAATGAA AGAAATTAAC CTTGCTCCCG ATAGCACATC TGTGGTCGTA 
TCAGGACTCA TGGTGGCCAC CAAGTATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CGGCTCAGGG AATTGTCACG ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG 
GCCCGCGTGA CAGATGCTAC TGAGACTACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACCATCACTG GCTTCCAGGT TGATGCCGTC CCAGCAAATG GCCAGCCTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TGCAACCCGG CACTGACTAC 
AAGATCTACT TGTACACCCT GAATGACAAT GCCCGGAGCT CCCCTGTGAT CATCGACGCC 
TCTACTGCCA TTGATGCACC ATCCAACCTG CATTTCCTGG CCACCACGCC CAACTCCTTG 
CTGATATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTATGAGAAG 
CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT CGGCCCCACC CTGGTGTCAC AGAGGCTACT 
ATTACTGGTC TGGAACCAGG AACCGAGTAC ACTATTCAAG TCATTGCCAT CAAGAACAAC 
CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA AAGACAGATG AGCTTCCCCA ACTGGTAACC 
CTTCCACACC CCAATCTTCA CGGACCAGAG ATCTTGGATG TTCCCTCCAC AGTTCAAAAG 
ACCCCTTTCA TCACCAACCC TGGGTATGAC AATGGAAACG GTATTCAGCT TCCTGGCACT 
TCTGGTCAGC AGCCCAGTGT TGGGCAACAA ATGATCTTTG AGGAGCATGG TTTTAGGCGA 
ACCACACCGC CCACAACGGC CACCCCCGTA AGGCATAGGC CAAGACCATA TCCGCCGAAT 
GTAAATGAGG AGATCCAAAT TGGTCATGTC CCCAGGGGAG ACGTAGACCA GCACCTCTAC 
CCTCACGTTC TGGGACTCAA TCCAAATACC TCTACAGGAC AAGAGGCTCT CTCTCAGACA 
ACCATCTCTT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT 
GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCCTG 
ACGGGTCTTA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCACTGAA AGACCAGAAG 
AGGCACAAGG TTCGGGAGGA GGTCGTTACT GTGGGCAACT CTGTTGACCA AGGCCTAGGC 
CAACCTACGG CTGACTCGTG CTTCGACCCC TACACCGTCT CCCATTACGC CATCGGAGAG 
GAGTGGGAGC GACTGTCTGA ATCTGGCTTT AAACTCTCCT GCCAGTGCTT AGGGTTTGGC 
AGTGGCCATT TCAGATGCGA TTCGTCTAAG TGGTGCCATG ACAATGGTGT GAACTACAAG 
ATTGGAGAGA AGTGGGATCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT 
GGAAATGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAAG CAACATGTTA TGATGACGGG 
AAGACGTACC ACGTAGGAGA ACAGTGGCAG AAGGAGTATC TTGGTGCTAT TTGCTCCTGC 
ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA 
CCCGGTCACG AAGGCTCCAC TGGGCACTCC TACAACCAGT ATTCACAGAG ATACCAGCAG 
AGAACCAACA CCAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT 
GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498049.1
CDS	267..7430
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTTIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642281.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642281.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCACCATTC CTGCACCAAC TGAACTCAAG TTCACTCAGG TTACACCAAC AAGCCTGACC 
GCCCAGTGGA CAGCACCCAA TGTTCAGCTC ACTGGGTACC GCGTGCGGGT GACCCCCAAG 
GAAAAGACTG GACCAATGAA AGAAATTAAC CTTGCTCCCG ATAGCACATC TGTGGTCGTA 
TCAGGACTCA TGGTGGCCAC CAAGTATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CGGCTCAGGG AATTGTCACG ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG 
GCCCGCGTGA CAGATGCTAC TGAGACTACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACCATCACTG GCTTCCAGGT TGATGCCGTC CCAGCAAATG GCCAGCCTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TGCAACCCGG CACTGACTAC 
AAGATCTACT TGTACACCCT GAATGACAAT GCCCGGAGCT CCCCTGTGAT CATCGACGCC 
TCTACTGCCA TTGATGCACC ATCCAACCTG CATTTCCTGG CCACCACGCC CAACTCCTTG 
CTGATATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTATGAGAAG 
CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT CGGCCCCACC CTGGTGTCAC AGAGGCTACT 
ATTACTGGTC TGGAACCAGG AACCGAGTAC ACTATTCAAG TCATTGCCAT CAAGAACAAC 
CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA AAGACAGATG AGCTTCCCCA ACTGGTAACC 
CTTCCACACC CCAATCTTCA CGGACCAGAG ATCTTGGATG TTCCCTCCAC AGTTCAAAAG 
ACCCCTTTCA TCACCAACCC TGGGTATGAC AATGGAAACG GTATTCAGCT TCCTGGCACT 
TCTGGTCAGC AGCCCAGTGT TGGGCAACAA ATGATCTTTG AGGAGCATGG TTTTAGGCGA 
ACCACACCGC CCACAACGGC CACCCCCGTA AGGCATAGGC CAAGACCATA TCCGCCGAAT 
GTAAATGAGG AGATCCAAAT TGGTCATGTC CCCAGGGGAG ACGTAGACCA GCACCTCTAC 
CCTCACGTTC TGGGACTCAA TCCAAATACC TCTACAGGAC AAGAGGCTCT CTCTCAGACA 
ACCATCTCTT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT 
GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCCTG 
ACGGGTCTTA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCACTGAA AGACCAGAAG 
AGGCACAAGG TTCGGGAGGA GGTCGTTACT GTGGGCAACT CTGTTGACCA AGGCCTAGGC 
CAACCTACGG CTGACTCGTG CTTCGACCCC TACACCGTCT CCCATTACGC CATCGGAGAG 
GAGTGGGAGC GACTGTCTGA ATCTGGCTTT AAACTCTCCT GCCAGTGCTT AGGGTTTGGC 
AGTGGCCATT TCAGATGCGA TTCGTCTAAG TGGTGCCATG ACAATGGTGT GAACTACAAG 
ATTGGAGAGA AGTGGGATCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT 
GGAAATGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAAG CAACATGTTA TGATGACGGG 
AAGACGTACC ACGTAGGAGA ACAGTGGCAG AAGGAGTATC TTGGTGCTAT TTGCTCCTGC 
ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA 
CCCGGTCACG AAGGCTCCAC TGGGCACTCC TACAACCAGT ATTCACAGAG ATACCAGCAG 
AGAACCAACA CCAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT 
GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642282.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498050.1 Equus caballus fibronectin 1 (FN1), transcript variant X4, mRNA.

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CloneID	OHb66103
Clone ID Related Accession (Same CDS sequence)	XM_023642282.1
Accession Version	XM_023642282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC AACATTGATC GCCCTAGAGG ACTGGCATTC 
ACAGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGGC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCCATTCCTG CACCAACTGA ACTCAAGTTC ACTCAGGTTA CACCAACAAG CCTGACCGCC 
CAGTGGACAG CACCCAATGT TCAGCTCACT GGGTACCGCG TGCGGGTGAC CCCCAAGGAA 
AAGACTGGAC CAATGAAAGA AATTAACCTT GCTCCCGATA GCACATCTGT GGTCGTATCA 
GGACTCATGG TGGCCACCAA GTATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCGG CTCAGGGAAT TGTCACGACC CTGGAGAATG TCAGCCCTCC AAGAAGGGCC 
CGCGTGACAG ATGCTACTGA GACTACCATC ACCATTAGCT GGAGAACCAA GACTGAGACC 
ATCACTGGCT TCCAGGTTGA TGCCGTCCCA GCAAATGGCC AGCCTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTGC AACCCGGCAC TGACTACAAG 
ATCTACTTGT ACACCCTGAA TGACAATGCC CGGAGCTCCC CTGTGATCAT CGACGCCTCT 
ACTGCCATTG ATGCACCATC CAACCTGCAT TTCCTGGCCA CCACGCCCAA CTCCTTGCTG 
ATATCATGGC AGCCACCCCG TGCCAGGATT ACTGGTTACA TCATCAAGTA TGAGAAGCCT 
GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCACCCTG GTGTCACAGA GGCTACTATT 
ACTGGTCTGG AACCAGGAAC CGAGTACACT ATTCAAGTCA TTGCCATCAA GAACAACCAG 
AAGAGCGAGC CTCTGATCGG GAGGAGAAAG ACAGATGAGC TTCCCCAACT GGTAACCCTT 
CCACACCCCA ATCTTCACGG ACCAGAGATC TTGGATGTTC CCTCCACAGT TCAAAAGACC 
CCTTTCATCA CCAACCCTGG GTATGACAAT GGAAACGGTA TTCAGCTTCC TGGCACTTCT 
GGTCAGCAGC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AGCATGGTTT TAGGCGAACC 
ACACCGCCCA CAACGGCCAC CCCCGTAAGG CATAGGCCAA GACCATATCC GCCGAATGTA 
AATGAGGAGA TCCAAATTGG TCATGTCCCC AGGGGAGACG TAGACCAGCA CCTCTACCCT 
CACGTTCTGG GACTCAATCC AAATACCTCT ACAGGACAAG AGGCTCTCTC TCAGACAACC 
ATCTCTTGGA CCCCATTCCA GGAAAGCTCT GAGTATATCA TTTCATGTCA TCCAGTTGGC 
ATTGATGAAG AACCCTTACA GTTCCGAGTT CCTGGAACCT CTGCTAGTGC CACCCTGACG 
GGTCTTACCA GAGGGGCCAC CTACAACATC ATAGTGGAGG CACTGAAAGA CCAGAAGAGG 
CACAAGGTTC GGGAGGAGGT CGTTACTGTG GGCAACTCTG TTGACCAAGG CCTAGGCCAA 
CCTACGGCTG ACTCGTGCTT CGACCCCTAC ACCGTCTCCC ATTACGCCAT CGGAGAGGAG 
TGGGAGCGAC TGTCTGAATC TGGCTTTAAA CTCTCCTGCC AGTGCTTAGG GTTTGGCAGT 
GGCCATTTCA GATGCGATTC GTCTAAGTGG TGCCATGACA ATGGTGTGAA CTACAAGATT 
GGAGAGAAGT GGGATCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGA 
AATGGAAAAG GAGAATTCAA GTGTGACCCT CATGAAGCAA CATGTTATGA TGACGGGAAG 
ACGTACCACG TAGGAGAACA GTGGCAGAAG GAGTATCTTG GTGCTATTTG CTCCTGCACA 
TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGCTGAACCC 
GGTCACGAAG GCTCCACTGG GCACTCCTAC AACCAGTATT CACAGAGATA CCAGCAGAGA 
ACCAACACCA ATGTCAACTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC 
AGAGATGATT CCCGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498050.1
CDS	267..7427
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTNIDRPRGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642282.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642282.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC AACATTGATC GCCCTAGAGG ACTGGCATTC 
ACAGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGGC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCCATTCCTG CACCAACTGA ACTCAAGTTC ACTCAGGTTA CACCAACAAG CCTGACCGCC 
CAGTGGACAG CACCCAATGT TCAGCTCACT GGGTACCGCG TGCGGGTGAC CCCCAAGGAA 
AAGACTGGAC CAATGAAAGA AATTAACCTT GCTCCCGATA GCACATCTGT GGTCGTATCA 
GGACTCATGG TGGCCACCAA GTATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCGG CTCAGGGAAT TGTCACGACC CTGGAGAATG TCAGCCCTCC AAGAAGGGCC 
CGCGTGACAG ATGCTACTGA GACTACCATC ACCATTAGCT GGAGAACCAA GACTGAGACC 
ATCACTGGCT TCCAGGTTGA TGCCGTCCCA GCAAATGGCC AGCCTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTGC AACCCGGCAC TGACTACAAG 
ATCTACTTGT ACACCCTGAA TGACAATGCC CGGAGCTCCC CTGTGATCAT CGACGCCTCT 
ACTGCCATTG ATGCACCATC CAACCTGCAT TTCCTGGCCA CCACGCCCAA CTCCTTGCTG 
ATATCATGGC AGCCACCCCG TGCCAGGATT ACTGGTTACA TCATCAAGTA TGAGAAGCCT 
GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCACCCTG GTGTCACAGA GGCTACTATT 
ACTGGTCTGG AACCAGGAAC CGAGTACACT ATTCAAGTCA TTGCCATCAA GAACAACCAG 
AAGAGCGAGC CTCTGATCGG GAGGAGAAAG ACAGATGAGC TTCCCCAACT GGTAACCCTT 
CCACACCCCA ATCTTCACGG ACCAGAGATC TTGGATGTTC CCTCCACAGT TCAAAAGACC 
CCTTTCATCA CCAACCCTGG GTATGACAAT GGAAACGGTA TTCAGCTTCC TGGCACTTCT 
GGTCAGCAGC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AGCATGGTTT TAGGCGAACC 
ACACCGCCCA CAACGGCCAC CCCCGTAAGG CATAGGCCAA GACCATATCC GCCGAATGTA 
AATGAGGAGA TCCAAATTGG TCATGTCCCC AGGGGAGACG TAGACCAGCA CCTCTACCCT 
CACGTTCTGG GACTCAATCC AAATACCTCT ACAGGACAAG AGGCTCTCTC TCAGACAACC 
ATCTCTTGGA CCCCATTCCA GGAAAGCTCT GAGTATATCA TTTCATGTCA TCCAGTTGGC 
ATTGATGAAG AACCCTTACA GTTCCGAGTT CCTGGAACCT CTGCTAGTGC CACCCTGACG 
GGTCTTACCA GAGGGGCCAC CTACAACATC ATAGTGGAGG CACTGAAAGA CCAGAAGAGG 
CACAAGGTTC GGGAGGAGGT CGTTACTGTG GGCAACTCTG TTGACCAAGG CCTAGGCCAA 
CCTACGGCTG ACTCGTGCTT CGACCCCTAC ACCGTCTCCC ATTACGCCAT CGGAGAGGAG 
TGGGAGCGAC TGTCTGAATC TGGCTTTAAA CTCTCCTGCC AGTGCTTAGG GTTTGGCAGT 
GGCCATTTCA GATGCGATTC GTCTAAGTGG TGCCATGACA ATGGTGTGAA CTACAAGATT 
GGAGAGAAGT GGGATCGTCA GGGAGAAAAT GGCCAGATGA TGAGCTGCAC ATGTCTTGGA 
AATGGAAAAG GAGAATTCAA GTGTGACCCT CATGAAGCAA CATGTTATGA TGACGGGAAG 
ACGTACCACG TAGGAGAACA GTGGCAGAAG GAGTATCTTG GTGCTATTTG CTCCTGCACA 
TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GCAGACCTGG GGCTGAACCC 
GGTCACGAAG GCTCCACTGG GCACTCCTAC AACCAGTATT CACAGAGATA CCAGCAGAGA 
ACCAACACCA ATGTCAACTG CCCAATTGAG TGCTTCATGC CTTTAGATGT ACAGGCTGAC 
AGAGATGATT CCCGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642283.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498051.1 Equus caballus fibronectin 1 (FN1), transcript variant X5, mRNA.

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CloneID	OHb66104
Clone ID Related Accession (Same CDS sequence)	XM_023642283.1
Accession Version	XM_023642283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC AACATTGATC GCCCTAGAGG ACTGGCATTC 
ACAGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGGC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCCATTCCTG CACCAACTGA ACTCAAGTTC ACTCAGGTTA CACCAACAAG CCTGACCGCC 
CAGTGGACAG CACCCAATGT TCAGCTCACT GGGTACCGCG TGCGGGTGAC CCCCAAGGAA 
AAGACTGGAC CAATGAAAGA AATTAACCTT GCTCCCGATA GCACATCTGT GGTCGTATCA 
GGACTCATGG TGGCCACCAA GTATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCGG CTCAGGGAAT TGTCACGACC CTGGAGAATG TCAGCCCTCC AAGAAGGGCC 
CGCGTGACAG ATGCTACTGA GACTACCATC ACCATTAGCT GGAGAACCAA GACTGAGACC 
ATCACTGGCT TCCAGGTTGA TGCCGTCCCA GCAAATGGCC AGCCTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTGC AACCCGGCAC TGACTACAAG 
ATCTACTTGT ACACCCTGAA TGACAATGCC CGGAGCTCCC CTGTGATCAT CGACGCCTCT 
ACTGCCATTG ATGCACCATC CAACCTGCAT TTCCTGGCCA CCACGCCCAA CTCCTTGCTG 
ATATCATGGC AGCCACCCCG TGCCAGGATT ACTGGTTACA TCATCAAGTA TGAGAAGCCT 
GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCACCCTG GTGTCACAGA GGCTACTATT 
ACTGGTCTGG AACCAGGAAC CGAGTACACT ATTCAAGTCA TTGCCATCAA GAACAACCAG 
AAGAGCGAGC CTCTGATCGG GAGGAGAAAG ACAGTTCAAA AGACCCCTTT CATCACCAAC 
CCTGGGTATG ACAATGGAAA CGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAGCCCAGT 
GTTGGGCAAC AAATGATCTT TGAGGAGCAT GGTTTTAGGC GAACCACACC GCCCACAACG 
GCCACCCCCG TAAGGCATAG GCCAAGACCA TATCCGCCGA ATGTAAATGA GGAGATCCAA 
ATTGGTCATG TCCCCAGGGG AGACGTAGAC CAGCACCTCT ACCCTCACGT TCTGGGACTC 
AATCCAAATA CCTCTACAGG ACAAGAGGCT CTCTCTCAGA CAACCATCTC TTGGACCCCA 
TTCCAGGAAA GCTCTGAGTA TATCATTTCA TGTCATCCAG TTGGCATTGA TGAAGAACCC 
TTACAGTTCC GAGTTCCTGG AACCTCTGCT AGTGCCACCC TGACGGGTCT TACCAGAGGG 
GCCACCTACA ACATCATAGT GGAGGCACTG AAAGACCAGA AGAGGCACAA GGTTCGGGAG 
GAGGTCGTTA CTGTGGGCAA CTCTGTTGAC CAAGGCCTAG GCCAACCTAC GGCTGACTCG 
TGCTTCGACC CCTACACCGT CTCCCATTAC GCCATCGGAG AGGAGTGGGA GCGACTGTCT 
GAATCTGGCT TTAAACTCTC CTGCCAGTGC TTAGGGTTTG GCAGTGGCCA TTTCAGATGC 
GATTCGTCTA AGTGGTGCCA TGACAATGGT GTGAACTACA AGATTGGAGA GAAGTGGGAT 
CGTCAGGGAG AAAATGGCCA GATGATGAGC TGCACATGTC TTGGAAATGG AAAAGGAGAA 
TTCAAGTGTG ACCCTCATGA AGCAACATGT TATGATGACG GGAAGACGTA CCACGTAGGA 
GAACAGTGGC AGAAGGAGTA TCTTGGTGCT ATTTGCTCCT GCACATGCTT TGGAGGCCAG 
CGGGGCTGGC GCTGTGACAA CTGCCGCAGA CCTGGGGCTG AACCCGGTCA CGAAGGCTCC 
ACTGGGCACT CCTACAACCA GTATTCACAG AGATACCAGC AGAGAACCAA CACCAATGTC 
AACTGCCCAA TTGAGTGCTT CATGCCTTTA GATGTACAGG CTGACAGAGA TGATTCCCGA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498051.1
CDS	267..7352
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTNIDRPRGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642283.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642283.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC AACATTGATC GCCCTAGAGG ACTGGCATTC 
ACAGATGTGG ATGTCGATTC CATCAAAATT GCTTGGGAAA GCCCACAGGG GCAAGTTTCC 
AGGTACAGGG TGACCTACTC GAGCCCTGAG GATGGAATCC ATGAGCTATT CCCTGCACCT 
GATGGTGAAG AAGACACTGC AGAGCTGCAA GGCCTCAGGC CGGGTTCTGA GTACACAGTC 
AGTGTGGTTG CCTTGCACGA TGATATGGAG AGCCAGCCCC TGATTGGAAC CCAGTCCACA 
GCCATTCCTG CACCAACTGA ACTCAAGTTC ACTCAGGTTA CACCAACAAG CCTGACCGCC 
CAGTGGACAG CACCCAATGT TCAGCTCACT GGGTACCGCG TGCGGGTGAC CCCCAAGGAA 
AAGACTGGAC CAATGAAAGA AATTAACCTT GCTCCCGATA GCACATCTGT GGTCGTATCA 
GGACTCATGG TGGCCACCAA GTATGAAGTG AGTGTCTATG CTCTTAAGGA CACTTTGACA 
AGCAGACCGG CTCAGGGAAT TGTCACGACC CTGGAGAATG TCAGCCCTCC AAGAAGGGCC 
CGCGTGACAG ATGCTACTGA GACTACCATC ACCATTAGCT GGAGAACCAA GACTGAGACC 
ATCACTGGCT TCCAGGTTGA TGCCGTCCCA GCAAATGGCC AGCCTCCAAT CCAGAGAACC 
ATCAAGCCAG ATGTCAGAAG CTACACCATC ACAGGTTTGC AACCCGGCAC TGACTACAAG 
ATCTACTTGT ACACCCTGAA TGACAATGCC CGGAGCTCCC CTGTGATCAT CGACGCCTCT 
ACTGCCATTG ATGCACCATC CAACCTGCAT TTCCTGGCCA CCACGCCCAA CTCCTTGCTG 
ATATCATGGC AGCCACCCCG TGCCAGGATT ACTGGTTACA TCATCAAGTA TGAGAAGCCT 
GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCACCCTG GTGTCACAGA GGCTACTATT 
ACTGGTCTGG AACCAGGAAC CGAGTACACT ATTCAAGTCA TTGCCATCAA GAACAACCAG 
AAGAGCGAGC CTCTGATCGG GAGGAGAAAG ACAGTTCAAA AGACCCCTTT CATCACCAAC 
CCTGGGTATG ACAATGGAAA CGGTATTCAG CTTCCTGGCA CTTCTGGTCA GCAGCCCAGT 
GTTGGGCAAC AAATGATCTT TGAGGAGCAT GGTTTTAGGC GAACCACACC GCCCACAACG 
GCCACCCCCG TAAGGCATAG GCCAAGACCA TATCCGCCGA ATGTAAATGA GGAGATCCAA 
ATTGGTCATG TCCCCAGGGG AGACGTAGAC CAGCACCTCT ACCCTCACGT TCTGGGACTC 
AATCCAAATA CCTCTACAGG ACAAGAGGCT CTCTCTCAGA CAACCATCTC TTGGACCCCA 
TTCCAGGAAA GCTCTGAGTA TATCATTTCA TGTCATCCAG TTGGCATTGA TGAAGAACCC 
TTACAGTTCC GAGTTCCTGG AACCTCTGCT AGTGCCACCC TGACGGGTCT TACCAGAGGG 
GCCACCTACA ACATCATAGT GGAGGCACTG AAAGACCAGA AGAGGCACAA GGTTCGGGAG 
GAGGTCGTTA CTGTGGGCAA CTCTGTTGAC CAAGGCCTAG GCCAACCTAC GGCTGACTCG 
TGCTTCGACC CCTACACCGT CTCCCATTAC GCCATCGGAG AGGAGTGGGA GCGACTGTCT 
GAATCTGGCT TTAAACTCTC CTGCCAGTGC TTAGGGTTTG GCAGTGGCCA TTTCAGATGC 
GATTCGTCTA AGTGGTGCCA TGACAATGGT GTGAACTACA AGATTGGAGA GAAGTGGGAT 
CGTCAGGGAG AAAATGGCCA GATGATGAGC TGCACATGTC TTGGAAATGG AAAAGGAGAA 
TTCAAGTGTG ACCCTCATGA AGCAACATGT TATGATGACG GGAAGACGTA CCACGTAGGA 
GAACAGTGGC AGAAGGAGTA TCTTGGTGCT ATTTGCTCCT GCACATGCTT TGGAGGCCAG 
CGGGGCTGGC GCTGTGACAA CTGCCGCAGA CCTGGGGCTG AACCCGGTCA CGAAGGCTCC 
ACTGGGCACT CCTACAACCA GTATTCACAG AGATACCAGC AGAGAACCAA CACCAATGTC 
AACTGCCCAA TTGAGTGCTT CATGCCTTTA GATGTACAGG CTGACAGAGA TGATTCCCGA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642284.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498052.1 Equus caballus fibronectin 1 (FN1), transcript variant X6, mRNA.

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CloneID	OHb66105
Clone ID Related Accession (Same CDS sequence)	XM_023642284.1
Accession Version	XM_023642284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGGAC AAGAGGCTCT CTCTCAGACA ACCATCTCTT GGACCCCATT CCAGGAAAGC 
TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA 
GTTCCTGGAA CCTCTGCTAG TGCCACCCTG ACGGGTCTTA CCAGAGGGGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGAAG AGGCACAAGG TTCGGGAGGA GGTCGTTACT 
GTGGGCAACT CTGTTGACCA AGGCCTAGGC CAACCTACGG CTGACTCGTG CTTCGACCCC 
TACACCGTCT CCCATTACGC CATCGGAGAG GAGTGGGAGC GACTGTCTGA ATCTGGCTTT 
AAACTCTCCT GCCAGTGCTT AGGGTTTGGC AGTGGCCATT TCAGATGCGA TTCGTCTAAG 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAAG CAACATGTTA TGATGACGGG AAGACGTACC ACGTAGGAGA ACAGTGGCAG 
AAGGAGTATC TTGGTGCTAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTCACG AAGGCTCCAC TGGGCACTCC 
TACAACCAGT ATTCACAGAG ATACCAGCAG AGAACCAACA CCAATGTCAA CTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498052.1
CDS	268..7341
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTNIDRPRGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642284.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642284.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGGAC AAGAGGCTCT CTCTCAGACA ACCATCTCTT GGACCCCATT CCAGGAAAGC 
TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA 
GTTCCTGGAA CCTCTGCTAG TGCCACCCTG ACGGGTCTTA CCAGAGGGGC CACCTACAAC 
ATCATAGTGG AGGCACTGAA AGACCAGAAG AGGCACAAGG TTCGGGAGGA GGTCGTTACT 
GTGGGCAACT CTGTTGACCA AGGCCTAGGC CAACCTACGG CTGACTCGTG CTTCGACCCC 
TACACCGTCT CCCATTACGC CATCGGAGAG GAGTGGGAGC GACTGTCTGA ATCTGGCTTT 
AAACTCTCCT GCCAGTGCTT AGGGTTTGGC AGTGGCCATT TCAGATGCGA TTCGTCTAAG 
TGGTGCCATG ACAATGGTGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAA 
AATGGCCAGA TGATGAGCTG CACATGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAC 
CCTCATGAAG CAACATGTTA TGATGACGGG AAGACGTACC ACGTAGGAGA ACAGTGGCAG 
AAGGAGTATC TTGGTGCTAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC 
TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTCACG AAGGCTCCAC TGGGCACTCC 
TACAACCAGT ATTCACAGAG ATACCAGCAG AGAACCAACA CCAATGTCAA CTGCCCAATT 
GAGTGCTTCA TGCCTTTAGA TGTACAGGCT GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642285.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498053.1 Equus caballus fibronectin 1 (FN1), transcript variant X7, mRNA.

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CloneID	OHb66106
Clone ID Related Accession (Same CDS sequence)	XM_023642285.1
Accession Version	XM_023642285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG CCATTCCTGC ACCAACTGAA CTCAAGTTCA CTCAGGTTAC ACCAACAAGC 
CTGACCGCCC AGTGGACAGC ACCCAATGTT CAGCTCACTG GGTACCGCGT GCGGGTGACC 
CCCAAGGAAA AGACTGGACC AATGAAAGAA ATTAACCTTG CTCCCGATAG CACATCTGTG 
GTCGTATCAG GACTCATGGT GGCCACCAAG TATGAAGTGA GTGTCTATGC TCTTAAGGAC 
ACTTTGACAA GCAGACCGGC TCAGGGAATT GTCACGACCC TGGAGAATGT CAGCCCTCCA 
AGAAGGGCCC GCGTGACAGA TGCTACTGAG ACTACCATCA CCATTAGCTG GAGAACCAAG 
ACTGAGACCA TCACTGGCTT CCAGGTTGAT GCCGTCCCAG CAAATGGCCA GCCTCCAATC 
CAGAGAACCA TCAAGCCAGA TGTCAGAAGC TACACCATCA CAGGTTTGCA ACCCGGCACT 
GACTACAAGA TCTACTTGTA CACCCTGAAT GACAATGCCC GGAGCTCCCC TGTGATCATC 
GACGCCTCTA CTGCCATTGA TGCACCATCC AACCTGCATT TCCTGGCCAC CACGCCCAAC 
TCCTTGCTGA TATCATGGCA GCCACCCCGT GCCAGGATTA CTGGTTACAT CATCAAGTAT 
GAGAAGCCTG GGTCCCCTCC CAGAGAAGTG GTCCCTCGGC CCCACCCTGG TGTCACAGAG 
GCTACTATTA CTGGTCTGGA ACCAGGAACC GAGTACACTA TTCAAGTCAT TGCCATCAAG 
AACAACCAGA AGAGCGAGCC TCTGATCGGG AGGAGAAAGA CAGATGAGCT TCCCCAACTG 
GTAACCCTTC CACACCCCAA TCTTCACGGA CCAGAGATCT TGGATGTTCC CTCCACAGTT 
CAAAAGACCC CTTTCATCAC CAACCCTGGG TATGACAATG GAAACGGTAT TCAGCTTCCT 
GGCACTTCTG GTCAGCAGCC CAGTGTTGGG CAACAAATGA TCTTTGAGGA GCATGGTTTT 
AGGCGAACCA CACCGCCCAC AACGGCCACC CCCGTAAGGC ATAGGCCAAG ACCATATCCG 
CCGAATGTAA ATGAGGAGAT CCAAATTGGT CATGTCCCCA GGGGAGACGT AGACCAGCAC 
CTCTACCCTC ACGTTCTGGG ACTCAATCCA AATACCTCTA CAGGACAAGA GGCTCTCTCT 
CAGACAACCA TCTCTTGGAC CCCATTCCAG GAAAGCTCTG AGTATATCAT TTCATGTCAT 
CCAGTTGGCA TTGATGAAGA ACCCTTACAG TTCCGAGTTC CTGGAACCTC TGCTAGTGCC 
ACCCTGACGG GTCTTACCAG AGGGGCCACC TACAACATCA TAGTGGAGGC ACTGAAAGAC 
CAGAAGAGGC ACAAGGTTCG GGAGGAGGTC GTTACTGTGG GCAACTCTGT TGACCAAGGC 
CTAGGCCAAC CTACGGCTGA CTCGTGCTTC GACCCCTACA CCGTCTCCCA TTACGCCATC 
GGAGAGGAGT GGGAGCGACT GTCTGAATCT GGCTTTAAAC TCTCCTGCCA GTGCTTAGGG 
TTTGGCAGTG GCCATTTCAG ATGCGATTCG TCTAAGTGGT GCCATGACAA TGGTGTGAAC 
TACAAGATTG GAGAGAAGTG GGATCGTCAG GGAGAAAATG GCCAGATGAT GAGCTGCACA 
TGTCTTGGAA ATGGAAAAGG AGAATTCAAG TGTGACCCTC ATGAAGCAAC ATGTTATGAT 
GACGGGAAGA CGTACCACGT AGGAGAACAG TGGCAGAAGG AGTATCTTGG TGCTATTTGC 
TCCTGCACAT GCTTTGGAGG CCAGCGGGGC TGGCGCTGTG ACAACTGCCG CAGACCTGGG 
GCTGAACCCG GTCACGAAGG CTCCACTGGG CACTCCTACA ACCAGTATTC ACAGAGATAC 
CAGCAGAGAA CCAACACCAA TGTCAACTGC CCAATTGAGT GCTTCATGCC TTTAGATGTA 
CAGGCTGACA GAGATGATTC CCGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498053.1
CDS	268..7317
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642285.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642285.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG CCATTCCTGC ACCAACTGAA CTCAAGTTCA CTCAGGTTAC ACCAACAAGC 
CTGACCGCCC AGTGGACAGC ACCCAATGTT CAGCTCACTG GGTACCGCGT GCGGGTGACC 
CCCAAGGAAA AGACTGGACC AATGAAAGAA ATTAACCTTG CTCCCGATAG CACATCTGTG 
GTCGTATCAG GACTCATGGT GGCCACCAAG TATGAAGTGA GTGTCTATGC TCTTAAGGAC 
ACTTTGACAA GCAGACCGGC TCAGGGAATT GTCACGACCC TGGAGAATGT CAGCCCTCCA 
AGAAGGGCCC GCGTGACAGA TGCTACTGAG ACTACCATCA CCATTAGCTG GAGAACCAAG 
ACTGAGACCA TCACTGGCTT CCAGGTTGAT GCCGTCCCAG CAAATGGCCA GCCTCCAATC 
CAGAGAACCA TCAAGCCAGA TGTCAGAAGC TACACCATCA CAGGTTTGCA ACCCGGCACT 
GACTACAAGA TCTACTTGTA CACCCTGAAT GACAATGCCC GGAGCTCCCC TGTGATCATC 
GACGCCTCTA CTGCCATTGA TGCACCATCC AACCTGCATT TCCTGGCCAC CACGCCCAAC 
TCCTTGCTGA TATCATGGCA GCCACCCCGT GCCAGGATTA CTGGTTACAT CATCAAGTAT 
GAGAAGCCTG GGTCCCCTCC CAGAGAAGTG GTCCCTCGGC CCCACCCTGG TGTCACAGAG 
GCTACTATTA CTGGTCTGGA ACCAGGAACC GAGTACACTA TTCAAGTCAT TGCCATCAAG 
AACAACCAGA AGAGCGAGCC TCTGATCGGG AGGAGAAAGA CAGATGAGCT TCCCCAACTG 
GTAACCCTTC CACACCCCAA TCTTCACGGA CCAGAGATCT TGGATGTTCC CTCCACAGTT 
CAAAAGACCC CTTTCATCAC CAACCCTGGG TATGACAATG GAAACGGTAT TCAGCTTCCT 
GGCACTTCTG GTCAGCAGCC CAGTGTTGGG CAACAAATGA TCTTTGAGGA GCATGGTTTT 
AGGCGAACCA CACCGCCCAC AACGGCCACC CCCGTAAGGC ATAGGCCAAG ACCATATCCG 
CCGAATGTAA ATGAGGAGAT CCAAATTGGT CATGTCCCCA GGGGAGACGT AGACCAGCAC 
CTCTACCCTC ACGTTCTGGG ACTCAATCCA AATACCTCTA CAGGACAAGA GGCTCTCTCT 
CAGACAACCA TCTCTTGGAC CCCATTCCAG GAAAGCTCTG AGTATATCAT TTCATGTCAT 
CCAGTTGGCA TTGATGAAGA ACCCTTACAG TTCCGAGTTC CTGGAACCTC TGCTAGTGCC 
ACCCTGACGG GTCTTACCAG AGGGGCCACC TACAACATCA TAGTGGAGGC ACTGAAAGAC 
CAGAAGAGGC ACAAGGTTCG GGAGGAGGTC GTTACTGTGG GCAACTCTGT TGACCAAGGC 
CTAGGCCAAC CTACGGCTGA CTCGTGCTTC GACCCCTACA CCGTCTCCCA TTACGCCATC 
GGAGAGGAGT GGGAGCGACT GTCTGAATCT GGCTTTAAAC TCTCCTGCCA GTGCTTAGGG 
TTTGGCAGTG GCCATTTCAG ATGCGATTCG TCTAAGTGGT GCCATGACAA TGGTGTGAAC 
TACAAGATTG GAGAGAAGTG GGATCGTCAG GGAGAAAATG GCCAGATGAT GAGCTGCACA 
TGTCTTGGAA ATGGAAAAGG AGAATTCAAG TGTGACCCTC ATGAAGCAAC ATGTTATGAT 
GACGGGAAGA CGTACCACGT AGGAGAACAG TGGCAGAAGG AGTATCTTGG TGCTATTTGC 
TCCTGCACAT GCTTTGGAGG CCAGCGGGGC TGGCGCTGTG ACAACTGCCG CAGACCTGGG 
GCTGAACCCG GTCACGAAGG CTCCACTGGG CACTCCTACA ACCAGTATTC ACAGAGATAC 
CAGCAGAGAA CCAACACCAA TGTCAACTGC CCAATTGAGT GCTTCATGCC TTTAGATGTA 
CAGGCTGACA GAGATGATTC CCGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642286.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498054.1 Equus caballus fibronectin 1 (FN1), transcript variant X8, mRNA.

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CloneID	OHb66107
Clone ID Related Accession (Same CDS sequence)	XM_023642286.1
Accession Version	XM_023642286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC ACCATTCCTG CACCAACTGA ACTCAAGTTC 
ACTCAGGTTA CACCAACAAG CCTGACCGCC CAGTGGACAG CACCCAATGT TCAGCTCACT 
GGGTACCGCG TGCGGGTGAC CCCCAAGGAA AAGACTGGAC CAATGAAAGA AATTAACCTT 
GCTCCCGATA GCACATCTGT GGTCGTATCA GGACTCATGG TGGCCACCAA GTATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCGG CTCAGGGAAT TGTCACGACC 
CTGGAGAATG TCAGCCCTCC AAGAAGGGCC CGCGTGACAG ATGCTACTGA GACTACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACC ATCACTGGCT TCCAGGTTGA TGCCGTCCCA 
GCAAATGGCC AGCCTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTGC AACCCGGCAC TGACTACAAG ATCTACTTGT ACACCCTGAA TGACAATGCC 
CGGAGCTCCC CTGTGATCAT CGACGCCTCT ACTGCCATTG ATGCACCATC CAACCTGCAT 
TTCCTGGCCA CCACGCCCAA CTCCTTGCTG ATATCATGGC AGCCACCCCG TGCCAGGATT 
ACTGGTTACA TCATCAAGTA TGAGAAGCCT GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCACCCTG GTGTCACAGA GGCTACTATT ACTGGTCTGG AACCAGGAAC CGAGTACACT 
ATTCAAGTCA TTGCCATCAA GAACAACCAG AAGAGCGAGC CTCTGATCGG GAGGAGAAAG 
ACAGATGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCACGG ACCAGAGATC 
TTGGATGTTC CCTCCACAGT TCAAAAGACC CCTTTCATCA CCAACCCTGG GTATGACAAT 
GGAAACGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAGC CCAGTGTTGG GCAACAAATG 
ATCTTTGAGG AGCATGGTTT TAGGCGAACC ACACCGCCCA CAACGGCCAC CCCCGTAAGG 
CATAGGCCAA GACCATATCC GCCGAATGTA AATGAGGAGA TCCAAATTGG TCATGTCCCC 
AGGGGAGACG TAGACCAGCA CCTCTACCCT CACGTTCTGG GACTCAATCC AAATACCTCT 
ACAGGACAAG AGGCTCTCTC TCAGACAACC ATCTCTTGGA CCCCATTCCA GGAAAGCTCT 
GAGTATATCA TTTCATGTCA TCCAGTTGGC ATTGATGAAG AACCCTTACA GTTCCGAGTT 
CCTGGAACCT CTGCTAGTGC CACCCTGACG GGTCTTACCA GAGGGGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGAAGAGG CACAAGGTTC GGGAGGAGGT CGTTACTGTG 
GGCAACTCTG TTGACCAAGG CCTAGGCCAA CCTACGGCTG ACTCGTGCTT CGACCCCTAC 
ACCGTCTCCC ATTACGCCAT CGGAGAGGAG TGGGAGCGAC TGTCTGAATC TGGCTTTAAA 
CTCTCCTGCC AGTGCTTAGG GTTTGGCAGT GGCCATTTCA GATGCGATTC GTCTAAGTGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGATCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGA AATGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAAGCAA CATGTTATGA TGACGGGAAG ACGTACCACG TAGGAGAACA GTGGCAGAAG 
GAGTATCTTG GTGCTATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGCTGAACCC GGTCACGAAG GCTCCACTGG GCACTCCTAC 
AACCAGTATT CACAGAGATA CCAGCAGAGA ACCAACACCA ATGTCAACTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGATGATT CCCGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498054.1
CDS	227..7117
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTTIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642286.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642286.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC ACCATTCCTG CACCAACTGA ACTCAAGTTC 
ACTCAGGTTA CACCAACAAG CCTGACCGCC CAGTGGACAG CACCCAATGT TCAGCTCACT 
GGGTACCGCG TGCGGGTGAC CCCCAAGGAA AAGACTGGAC CAATGAAAGA AATTAACCTT 
GCTCCCGATA GCACATCTGT GGTCGTATCA GGACTCATGG TGGCCACCAA GTATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCGG CTCAGGGAAT TGTCACGACC 
CTGGAGAATG TCAGCCCTCC AAGAAGGGCC CGCGTGACAG ATGCTACTGA GACTACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACC ATCACTGGCT TCCAGGTTGA TGCCGTCCCA 
GCAAATGGCC AGCCTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTGC AACCCGGCAC TGACTACAAG ATCTACTTGT ACACCCTGAA TGACAATGCC 
CGGAGCTCCC CTGTGATCAT CGACGCCTCT ACTGCCATTG ATGCACCATC CAACCTGCAT 
TTCCTGGCCA CCACGCCCAA CTCCTTGCTG ATATCATGGC AGCCACCCCG TGCCAGGATT 
ACTGGTTACA TCATCAAGTA TGAGAAGCCT GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCACCCTG GTGTCACAGA GGCTACTATT ACTGGTCTGG AACCAGGAAC CGAGTACACT 
ATTCAAGTCA TTGCCATCAA GAACAACCAG AAGAGCGAGC CTCTGATCGG GAGGAGAAAG 
ACAGATGAGC TTCCCCAACT GGTAACCCTT CCACACCCCA ATCTTCACGG ACCAGAGATC 
TTGGATGTTC CCTCCACAGT TCAAAAGACC CCTTTCATCA CCAACCCTGG GTATGACAAT 
GGAAACGGTA TTCAGCTTCC TGGCACTTCT GGTCAGCAGC CCAGTGTTGG GCAACAAATG 
ATCTTTGAGG AGCATGGTTT TAGGCGAACC ACACCGCCCA CAACGGCCAC CCCCGTAAGG 
CATAGGCCAA GACCATATCC GCCGAATGTA AATGAGGAGA TCCAAATTGG TCATGTCCCC 
AGGGGAGACG TAGACCAGCA CCTCTACCCT CACGTTCTGG GACTCAATCC AAATACCTCT 
ACAGGACAAG AGGCTCTCTC TCAGACAACC ATCTCTTGGA CCCCATTCCA GGAAAGCTCT 
GAGTATATCA TTTCATGTCA TCCAGTTGGC ATTGATGAAG AACCCTTACA GTTCCGAGTT 
CCTGGAACCT CTGCTAGTGC CACCCTGACG GGTCTTACCA GAGGGGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGAAGAGG CACAAGGTTC GGGAGGAGGT CGTTACTGTG 
GGCAACTCTG TTGACCAAGG CCTAGGCCAA CCTACGGCTG ACTCGTGCTT CGACCCCTAC 
ACCGTCTCCC ATTACGCCAT CGGAGAGGAG TGGGAGCGAC TGTCTGAATC TGGCTTTAAA 
CTCTCCTGCC AGTGCTTAGG GTTTGGCAGT GGCCATTTCA GATGCGATTC GTCTAAGTGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGATCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGA AATGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAAGCAA CATGTTATGA TGACGGGAAG ACGTACCACG TAGGAGAACA GTGGCAGAAG 
GAGTATCTTG GTGCTATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGCTGAACCC GGTCACGAAG GCTCCACTGG GCACTCCTAC 
AACCAGTATT CACAGAGATA CCAGCAGAGA ACCAACACCA ATGTCAACTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGATGATT CCCGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642287.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498055.1 Equus caballus fibronectin 1 (FN1), transcript variant X9, mRNA.

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CloneID	OHb66108
Clone ID Related Accession (Same CDS sequence)	XM_023642287.1
Accession Version	XM_023642287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC ACCATTCCTG CACCAACTGA ACTCAAGTTC 
ACTCAGGTTA CACCAACAAG CCTGACCGCC CAGTGGACAG CACCCAATGT TCAGCTCACT 
GGGTACCGCG TGCGGGTGAC CCCCAAGGAA AAGACTGGAC CAATGAAAGA AATTAACCTT 
GCTCCCGATA GCACATCTGT GGTCGTATCA GGACTCATGG TGGCCACCAA GTATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCGG CTCAGGGAAT TGTCACGACC 
CTGGAGAATG TCAGCCCTCC AAGAAGGGCC CGCGTGACAG ATGCTACTGA GACTACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACC ATCACTGGCT TCCAGGTTGA TGCCGTCCCA 
GCAAATGGCC AGCCTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTGC AACCCGGCAC TGACTACAAG ATCTACTTGT ACACCCTGAA TGACAATGCC 
CGGAGCTCCC CTGTGATCAT CGACGCCTCT ACTGCCATTG ATGCACCATC CAACCTGCAT 
TTCCTGGCCA CCACGCCCAA CTCCTTGCTG ATATCATGGC AGCCACCCCG TGCCAGGATT 
ACTGGTTACA TCATCAAGTA TGAGAAGCCT GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCACCCTG GTGTCACAGA GGCTACTATT ACTGGTCTGG AACCAGGAAC CGAGTACACT 
ATTCAAGTCA TTGCCATCAA GAACAACCAG AAGAGCGAGC CTCTGATCGG GAGGAGAAAG 
ACAGTTCAAA AGACCCCTTT CATCACCAAC CCTGGGTATG ACAATGGAAA CGGTATTCAG 
CTTCCTGGCA CTTCTGGTCA GCAGCCCAGT GTTGGGCAAC AAATGATCTT TGAGGAGCAT 
GGTTTTAGGC GAACCACACC GCCCACAACG GCCACCCCCG TAAGGCATAG GCCAAGACCA 
TATCCGCCGA ATGTAAATGA GGAGATCCAA ATTGGTCATG TCCCCAGGGG AGACGTAGAC 
CAGCACCTCT ACCCTCACGT TCTGGGACTC AATCCAAATA CCTCTACAGG ACAAGAGGCT 
CTCTCTCAGA CAACCATCTC TTGGACCCCA TTCCAGGAAA GCTCTGAGTA TATCATTTCA 
TGTCATCCAG TTGGCATTGA TGAAGAACCC TTACAGTTCC GAGTTCCTGG AACCTCTGCT 
AGTGCCACCC TGACGGGTCT TACCAGAGGG GCCACCTACA ACATCATAGT GGAGGCACTG 
AAAGACCAGA AGAGGCACAA GGTTCGGGAG GAGGTCGTTA CTGTGGGCAA CTCTGTTGAC 
CAAGGCCTAG GCCAACCTAC GGCTGACTCG TGCTTCGACC CCTACACCGT CTCCCATTAC 
GCCATCGGAG AGGAGTGGGA GCGACTGTCT GAATCTGGCT TTAAACTCTC CTGCCAGTGC 
TTAGGGTTTG GCAGTGGCCA TTTCAGATGC GATTCGTCTA AGTGGTGCCA TGACAATGGT 
GTGAACTACA AGATTGGAGA GAAGTGGGAT CGTCAGGGAG AAAATGGCCA GATGATGAGC 
TGCACATGTC TTGGAAATGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA AGCAACATGT 
TATGATGACG GGAAGACGTA CCACGTAGGA GAACAGTGGC AGAAGGAGTA TCTTGGTGCT 
ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA 
CCTGGGGCTG AACCCGGTCA CGAAGGCTCC ACTGGGCACT CCTACAACCA GTATTCACAG 
AGATACCAGC AGAGAACCAA CACCAATGTC AACTGCCCAA TTGAGTGCTT CATGCCTTTA 
GATGTACAGG CTGACAGAGA TGATTCCCGA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498055.1
CDS	267..7082
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTTIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTVQKTPFITNPGYDNGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPVRHRPRPYPPNVNEEIQIGHVPRGDVDQHLYPHVLGLNPNTSTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642287.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642287.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC ACCATTCCTG CACCAACTGA ACTCAAGTTC 
ACTCAGGTTA CACCAACAAG CCTGACCGCC CAGTGGACAG CACCCAATGT TCAGCTCACT 
GGGTACCGCG TGCGGGTGAC CCCCAAGGAA AAGACTGGAC CAATGAAAGA AATTAACCTT 
GCTCCCGATA GCACATCTGT GGTCGTATCA GGACTCATGG TGGCCACCAA GTATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCGG CTCAGGGAAT TGTCACGACC 
CTGGAGAATG TCAGCCCTCC AAGAAGGGCC CGCGTGACAG ATGCTACTGA GACTACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACC ATCACTGGCT TCCAGGTTGA TGCCGTCCCA 
GCAAATGGCC AGCCTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTGC AACCCGGCAC TGACTACAAG ATCTACTTGT ACACCCTGAA TGACAATGCC 
CGGAGCTCCC CTGTGATCAT CGACGCCTCT ACTGCCATTG ATGCACCATC CAACCTGCAT 
TTCCTGGCCA CCACGCCCAA CTCCTTGCTG ATATCATGGC AGCCACCCCG TGCCAGGATT 
ACTGGTTACA TCATCAAGTA TGAGAAGCCT GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCACCCTG GTGTCACAGA GGCTACTATT ACTGGTCTGG AACCAGGAAC CGAGTACACT 
ATTCAAGTCA TTGCCATCAA GAACAACCAG AAGAGCGAGC CTCTGATCGG GAGGAGAAAG 
ACAGTTCAAA AGACCCCTTT CATCACCAAC CCTGGGTATG ACAATGGAAA CGGTATTCAG 
CTTCCTGGCA CTTCTGGTCA GCAGCCCAGT GTTGGGCAAC AAATGATCTT TGAGGAGCAT 
GGTTTTAGGC GAACCACACC GCCCACAACG GCCACCCCCG TAAGGCATAG GCCAAGACCA 
TATCCGCCGA ATGTAAATGA GGAGATCCAA ATTGGTCATG TCCCCAGGGG AGACGTAGAC 
CAGCACCTCT ACCCTCACGT TCTGGGACTC AATCCAAATA CCTCTACAGG ACAAGAGGCT 
CTCTCTCAGA CAACCATCTC TTGGACCCCA TTCCAGGAAA GCTCTGAGTA TATCATTTCA 
TGTCATCCAG TTGGCATTGA TGAAGAACCC TTACAGTTCC GAGTTCCTGG AACCTCTGCT 
AGTGCCACCC TGACGGGTCT TACCAGAGGG GCCACCTACA ACATCATAGT GGAGGCACTG 
AAAGACCAGA AGAGGCACAA GGTTCGGGAG GAGGTCGTTA CTGTGGGCAA CTCTGTTGAC 
CAAGGCCTAG GCCAACCTAC GGCTGACTCG TGCTTCGACC CCTACACCGT CTCCCATTAC 
GCCATCGGAG AGGAGTGGGA GCGACTGTCT GAATCTGGCT TTAAACTCTC CTGCCAGTGC 
TTAGGGTTTG GCAGTGGCCA TTTCAGATGC GATTCGTCTA AGTGGTGCCA TGACAATGGT 
GTGAACTACA AGATTGGAGA GAAGTGGGAT CGTCAGGGAG AAAATGGCCA GATGATGAGC 
TGCACATGTC TTGGAAATGG AAAAGGAGAA TTCAAGTGTG ACCCTCATGA AGCAACATGT 
TATGATGACG GGAAGACGTA CCACGTAGGA GAACAGTGGC AGAAGGAGTA TCTTGGTGCT 
ATTTGCTCCT GCACATGCTT TGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGCAGA 
CCTGGGGCTG AACCCGGTCA CGAAGGCTCC ACTGGGCACT CCTACAACCA GTATTCACAG 
AGATACCAGC AGAGAACCAA CACCAATGTC AACTGCCCAA TTGAGTGCTT CATGCCTTTA 
GATGTACAGG CTGACAGAGA TGATTCCCGA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642289.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498057.1 Equus caballus fibronectin 1 (FN1), transcript variant X10, mRNA.

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CloneID	OHb66109
Clone ID Related Accession (Same CDS sequence)	XM_023642289.1
Accession Version	XM_023642289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCACCATTC CTGCACCAAC TGAACTCAAG TTCACTCAGG TTACACCAAC AAGCCTGACC 
GCCCAGTGGA CAGCACCCAA TGTTCAGCTC ACTGGGTACC GCGTGCGGGT GACCCCCAAG 
GAAAAGACTG GACCAATGAA AGAAATTAAC CTTGCTCCCG ATAGCACATC TGTGGTCGTA 
TCAGGACTCA TGGTGGCCAC CAAGTATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CGGCTCAGGG AATTGTCACG ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG 
GCCCGCGTGA CAGATGCTAC TGAGACTACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACCATCACTG GCTTCCAGGT TGATGCCGTC CCAGCAAATG GCCAGCCTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TGCAACCCGG CACTGACTAC 
AAGATCTACT TGTACACCCT GAATGACAAT GCCCGGAGCT CCCCTGTGAT CATCGACGCC 
TCTACTGCCA TTGATGCACC ATCCAACCTG CATTTCCTGG CCACCACGCC CAACTCCTTG 
CTGATATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTATGAGAAG 
CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT CGGCCCCACC CTGGTGTCAC AGAGGCTACT 
ATTACTGGTC TGGAACCAGG AACCGAGTAC ACTATTCAAG TCATTGCCAT CAAGAACAAC 
CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA AAGACAGGAC AAGAGGCTCT CTCTCAGACA 
ACCATCTCTT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT 
GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCCTG 
ACGGGTCTTA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCACTGAA AGACCAGAAG 
AGGCACAAGG TTCGGGAGGA GGTCGTTACT GTGGGCAACT CTGTTGACCA AGGCCTAGGC 
CAACCTACGG CTGACTCGTG CTTCGACCCC TACACCGTCT CCCATTACGC CATCGGAGAG 
GAGTGGGAGC GACTGTCTGA ATCTGGCTTT AAACTCTCCT GCCAGTGCTT AGGGTTTGGC 
AGTGGCCATT TCAGATGCGA TTCGTCTAAG TGGTGCCATG ACAATGGTGT GAACTACAAG 
ATTGGAGAGA AGTGGGATCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT 
GGAAATGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAAG CAACATGTTA TGATGACGGG 
AAGACGTACC ACGTAGGAGA ACAGTGGCAG AAGGAGTATC TTGGTGCTAT TTGCTCCTGC 
ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA 
CCCGGTCACG AAGGCTCCAC TGGGCACTCC TACAACCAGT ATTCACAGAG ATACCAGCAG 
AGAACCAACA CCAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT 
GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498057.1
CDS	267..7070
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTTIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642289.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642289.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCACCATTC CTGCACCAAC TGAACTCAAG TTCACTCAGG TTACACCAAC AAGCCTGACC 
GCCCAGTGGA CAGCACCCAA TGTTCAGCTC ACTGGGTACC GCGTGCGGGT GACCCCCAAG 
GAAAAGACTG GACCAATGAA AGAAATTAAC CTTGCTCCCG ATAGCACATC TGTGGTCGTA 
TCAGGACTCA TGGTGGCCAC CAAGTATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG 
ACAAGCAGAC CGGCTCAGGG AATTGTCACG ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG 
GCCCGCGTGA CAGATGCTAC TGAGACTACC ATCACCATTA GCTGGAGAAC CAAGACTGAG 
ACCATCACTG GCTTCCAGGT TGATGCCGTC CCAGCAAATG GCCAGCCTCC AATCCAGAGA 
ACCATCAAGC CAGATGTCAG AAGCTACACC ATCACAGGTT TGCAACCCGG CACTGACTAC 
AAGATCTACT TGTACACCCT GAATGACAAT GCCCGGAGCT CCCCTGTGAT CATCGACGCC 
TCTACTGCCA TTGATGCACC ATCCAACCTG CATTTCCTGG CCACCACGCC CAACTCCTTG 
CTGATATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTATGAGAAG 
CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT CGGCCCCACC CTGGTGTCAC AGAGGCTACT 
ATTACTGGTC TGGAACCAGG AACCGAGTAC ACTATTCAAG TCATTGCCAT CAAGAACAAC 
CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA AAGACAGGAC AAGAGGCTCT CTCTCAGACA 
ACCATCTCTT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT 
GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCCTG 
ACGGGTCTTA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCACTGAA AGACCAGAAG 
AGGCACAAGG TTCGGGAGGA GGTCGTTACT GTGGGCAACT CTGTTGACCA AGGCCTAGGC 
CAACCTACGG CTGACTCGTG CTTCGACCCC TACACCGTCT CCCATTACGC CATCGGAGAG 
GAGTGGGAGC GACTGTCTGA ATCTGGCTTT AAACTCTCCT GCCAGTGCTT AGGGTTTGGC 
AGTGGCCATT TCAGATGCGA TTCGTCTAAG TGGTGCCATG ACAATGGTGT GAACTACAAG 
ATTGGAGAGA AGTGGGATCG TCAGGGAGAA AATGGCCAGA TGATGAGCTG CACATGTCTT 
GGAAATGGAA AAGGAGAATT CAAGTGTGAC CCTCATGAAG CAACATGTTA TGATGACGGG 
AAGACGTACC ACGTAGGAGA ACAGTGGCAG AAGGAGTATC TTGGTGCTAT TTGCTCCTGC 
ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA 
CCCGGTCACG AAGGCTCCAC TGGGCACTCC TACAACCAGT ATTCACAGAG ATACCAGCAG 
AGAACCAACA CCAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTAGA TGTACAGGCT 
GACAGAGATG ATTCCCGAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642290.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498058.1 Equus caballus fibronectin 1 (FN1), transcript variant X11, mRNA.

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CloneID	OHb66110
Clone ID Related Accession (Same CDS sequence)	XM_023642290.1
Accession Version	XM_023642290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGAGT GGTGCCATGA CAATGGTGTG AACTACAAGA TTGGAGAGAA GTGGGATCGT 
CAGGGAGAAA ATGGCCAGAT GATGAGCTGC ACATGTCTTG GAAATGGAAA AGGAGAATTC 
AAGTGTGACC CTCATGAAGC AACATGTTAT GATGACGGGA AGACGTACCA CGTAGGAGAA 
CAGTGGCAGA AGGAGTATCT TGGTGCTATT TGCTCCTGCA CATGCTTTGG AGGCCAGCGG 
GGCTGGCGCT GTGACAACTG CCGCAGACCT GGGGCTGAAC CCGGTCACGA AGGCTCCACT 
GGGCACTCCT ACAACCAGTA TTCACAGAGA TACCAGCAGA GAACCAACAC CAATGTCAAC 
TGCCCAATTG AGTGCTTCAT GCCTTTAGAT GTACAGGCTG ACAGAGATGA TTCCCGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498058.1
CDS	268..6930
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTNIDRPRGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTEWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642290.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642290.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGAGGT GCCCCAACTC ACTGACCTAA GCTTTGTTGA TATAACCGAT 
TCAAGCATCG GCCTGAGGTG GACCCCGCTA AACTCTTCCA CCATTATTGG GTATCGCATC 
ACAGTAGTTG CGGCAGGAGA AGGTATCCCT ATTTTTGAAG ATTTTGTGGA CTCCTCAGTA 
GGATACTACA CAGTTACAGG GCTGGAGCCT GGCATTGACT ATGACATCAG CGTTATCACT 
CTCATTAATG GCGGAGAGAG TGCCCCTACT ACGCTGACAC AACAAACCGC CGTCCCTCCT 
CCCACTGACC TGCGATTCAC CAATGTTGGT CCAGACACTA TGCGTGTCAC CTGGGCCCCA 
CCTCCATCTA TTGAATTGAC CAACCTCCTG GTGCGCTACT CACCCGTGAA AAATGAGGAA 
GATGTTGCGG AATTGTCAAT TTCTCCTTCA GACAATGCAG TGGTCTTAAC AAATCTCCTG 
CCTGGGACAG AATACTTAGT CAGTGTCTCC AGCGTTTACG AACAACACGA GAGCACACCT 
CTTAGAGGAA GACAGAAGAC AGGTCTTGAT TCCCCAACTG GCATTGACTT TTCTGATATC 
ACTGCCAACT CTTTCACGGT CCATTGGATT GCTCCTCGAG CCACCATCAC TGGCTACAGG 
ATTCGCCATC ATCCTGAGCA CACTGGTGGA AGACCACGAG AGGATCGAGT GCCTCCCTCT 
CGGAATTCTA TCACCCTCAC CAACCTCAAT CCAGGCACAG AATATGTGGT CAGCATCGTT 
GCTCTTAATG GCAGAGAGGA AAGTCCTCCC TTGGTTGGCC AACAATCAAC AGTTTCTGAC 
GTTCCAAGGG ACCTGGAAGT TATTGCCACA ACCCCCACCA GCATCTTGAT CAGCTGGGAC 
GCTCCTGCCG TCACAGTGAG ATATTACAGG ATCACCTATG GAGAAACAGG AGGAAATAGC 
CCTGTCCAGG AGTTCACTGT GCCTGGCAGC AAGTCTACAG CTACCATCAG TGGCCTTAAA 
CCTGGAGTAG ACTACACCAT CACCGTTTAT GCTGTCACTG GCCGCGGGGA CAGCCCAGCC 
AGCAGCAAGC CAATTTCCAT TGATTACCGA ACAGAAATTG ACAAACCATC CCAGATGCAA 
GTGACTGATG TTCAGGACAA CAGCATTAGT GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT 
ACTGGCTACA GAGTGACCAC TACTCCCAAA AATGGCCCAG GACAATCAAA AACTAAAACT 
GCAGGTCCAG ATCAAACAGA AATGACCATC GAAGGCTTAC AACCCACAGT GGAGTATGTG 
GTTAGTGTCT ATGCTCAGAA TCAAAACGGA GAGAGTCAGC CTCTGGTTCA GACAGCAGTA 
ACCAACATTG ATCGCCCTAG AGGACTGGCA TTCACAGATG TGGATGTCGA TTCCATCAAA 
ATTGCTTGGG AAAGCCCACA GGGGCAAGTT TCCAGGTACA GGGTGACCTA CTCGAGCCCT 
GAGGATGGAA TCCATGAGCT ATTCCCTGCA CCTGATGGTG AAGAAGACAC TGCAGAGCTG 
CAAGGCCTCA GGCCGGGTTC TGAGTACACA GTCAGTGTGG TTGCCTTGCA CGATGATATG 
GAGAGCCAGC CCCTGATTGG AACCCAGTCC ACAGCCATTC CTGCACCAAC TGAACTCAAG 
TTCACTCAGG TTACACCAAC AAGCCTGACC GCCCAGTGGA CAGCACCCAA TGTTCAGCTC 
ACTGGGTACC GCGTGCGGGT GACCCCCAAG GAAAAGACTG GACCAATGAA AGAAATTAAC 
CTTGCTCCCG ATAGCACATC TGTGGTCGTA TCAGGACTCA TGGTGGCCAC CAAGTATGAA 
GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AATTGTCACG 
ACCCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGCGTGA CAGATGCTAC TGAGACTACC 
ATCACCATTA GCTGGAGAAC CAAGACTGAG ACCATCACTG GCTTCCAGGT TGATGCCGTC 
CCAGCAAATG GCCAGCCTCC AATCCAGAGA ACCATCAAGC CAGATGTCAG AAGCTACACC 
ATCACAGGTT TGCAACCCGG CACTGACTAC AAGATCTACT TGTACACCCT GAATGACAAT 
GCCCGGAGCT CCCCTGTGAT CATCGACGCC TCTACTGCCA TTGATGCACC ATCCAACCTG 
CATTTCCTGG CCACCACGCC CAACTCCTTG CTGATATCAT GGCAGCCACC CCGTGCCAGG 
ATTACTGGTT ACATCATCAA GTATGAGAAG CCTGGGTCCC CTCCCAGAGA AGTGGTCCCT 
CGGCCCCACC CTGGTGTCAC AGAGGCTACT ATTACTGGTC TGGAACCAGG AACCGAGTAC 
ACTATTCAAG TCATTGCCAT CAAGAACAAC CAGAAGAGCG AGCCTCTGAT CGGGAGGAGA 
AAGACAGAGT GGTGCCATGA CAATGGTGTG AACTACAAGA TTGGAGAGAA GTGGGATCGT 
CAGGGAGAAA ATGGCCAGAT GATGAGCTGC ACATGTCTTG GAAATGGAAA AGGAGAATTC 
AAGTGTGACC CTCATGAAGC AACATGTTAT GATGACGGGA AGACGTACCA CGTAGGAGAA 
CAGTGGCAGA AGGAGTATCT TGGTGCTATT TGCTCCTGCA CATGCTTTGG AGGCCAGCGG 
GGCTGGCGCT GTGACAACTG CCGCAGACCT GGGGCTGAAC CCGGTCACGA AGGCTCCACT 
GGGCACTCCT ACAACCAGTA TTCACAGAGA TACCAGCAGA GAACCAACAC CAATGTCAAC 
TGCCCAATTG AGTGCTTCAT GCCTTTAGAT GTACAGGCTG ACAGAGATGA TTCCCGAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1 ( XM_023642291.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023498059.1 Equus caballus fibronectin 1 (FN1), transcript variant X12, mRNA.

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CloneID	OHb66111
Clone ID Related Accession (Same CDS sequence)	XM_023642291.1
Accession Version	XM_023642291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	fibronectin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC ACCATTCCTG CACCAACTGA ACTCAAGTTC 
ACTCAGGTTA CACCAACAAG CCTGACCGCC CAGTGGACAG CACCCAATGT TCAGCTCACT 
GGGTACCGCG TGCGGGTGAC CCCCAAGGAA AAGACTGGAC CAATGAAAGA AATTAACCTT 
GCTCCCGATA GCACATCTGT GGTCGTATCA GGACTCATGG TGGCCACCAA GTATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCGG CTCAGGGAAT TGTCACGACC 
CTGGAGAATG TCAGCCCTCC AAGAAGGGCC CGCGTGACAG ATGCTACTGA GACTACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACC ATCACTGGCT TCCAGGTTGA TGCCGTCCCA 
GCAAATGGCC AGCCTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTGC AACCCGGCAC TGACTACAAG ATCTACTTGT ACACCCTGAA TGACAATGCC 
CGGAGCTCCC CTGTGATCAT CGACGCCTCT ACTGCCATTG ATGCACCATC CAACCTGCAT 
TTCCTGGCCA CCACGCCCAA CTCCTTGCTG ATATCATGGC AGCCACCCCG TGCCAGGATT 
ACTGGTTACA TCATCAAGTA TGAGAAGCCT GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCACCCTG GTGTCACAGA GGCTACTATT ACTGGTCTGG AACCAGGAAC CGAGTACACT 
ATTCAAGTCA TTGCCATCAA GAACAACCAG AAGAGCGAGC CTCTGATCGG GAGGAGAAAG 
ACAGGACAAG AGGCTCTCTC TCAGACAACC ATCTCTTGGA CCCCATTCCA GGAAAGCTCT 
GAGTATATCA TTTCATGTCA TCCAGTTGGC ATTGATGAAG AACCCTTACA GTTCCGAGTT 
CCTGGAACCT CTGCTAGTGC CACCCTGACG GGTCTTACCA GAGGGGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGAAGAGG CACAAGGTTC GGGAGGAGGT CGTTACTGTG 
GGCAACTCTG TTGACCAAGG CCTAGGCCAA CCTACGGCTG ACTCGTGCTT CGACCCCTAC 
ACCGTCTCCC ATTACGCCAT CGGAGAGGAG TGGGAGCGAC TGTCTGAATC TGGCTTTAAA 
CTCTCCTGCC AGTGCTTAGG GTTTGGCAGT GGCCATTTCA GATGCGATTC GTCTAAGTGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGATCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGA AATGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAAGCAA CATGTTATGA TGACGGGAAG ACGTACCACG TAGGAGAACA GTGGCAGAAG 
GAGTATCTTG GTGCTATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGCTGAACCC GGTCACGAAG GCTCCACTGG GCACTCCTAC 
AACCAGTATT CACAGAGATA CCAGCAGAGA ACCAACACCA ATGTCAACTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGATGATT CCCGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023498059.1
CDS	266..6796
Translation	MLRGPGPGLLLLVVLSLGTAVPSSGASKRKRQDQQIIQPQSPVAVGQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHASLQTTSTGSGPFTDVRTAIYQPQPHPQPAPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDSAVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRVGDQWDKQHDMGHMMRCTCVGNGRGEWTCVAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQCQDSETRTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQWNAPEPSHISKYILRWKPKNAPGRWKEATIPGHLNSYTIKGLRPGVVYEGQLISVQHYGHKEVTRFDFTTTSTSPAVTSNTVTGETTPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEEGEQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELTLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRADKVPPPRDLQFVEVTDVKITIMWTPPESAVTGYRVDVLPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYHFKIFAVNHGRESKPLTGEQTTKLDAPTNLRFINETESTVIVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPSASQYPLRSLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQSPGSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTISVLRDGQERDAPIVKKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTSGQQGYSLEEVVHADQSSCTFENLSPGLEYNVSVYTVKDDKESVPISDTIIPAVPPPTDLRFTNVGPDTMRVTWAPPPSIELTNLLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHTGGRPREDRVPPSRNSITLTNLNPGTEYVVSIVALNGREESPPLVGQQSTVSDVPRDLEVIATTPTSILISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISIDYRTEIDKPSQMQVTDVQDNSISVRWLPSSSPVTGYRVTTTPKNGPGQSKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNQNGESQPLVQTAVTTIPAPTELKFTQVTPTSLTAQWTAPNVQLTGYRVRVTPKEKTGPMKEINLAPDSTSVVVSGLMVATKYEVSVYALKDTLTSRPAQGIVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQPPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVIIDASTAIDAPSNLHFLATTPNSLLISWQPPRARITGYIIKYEKPGSPPREVVPRPHPGVTEATITGLEPGTEYTIQVIAIKNNQKSEPLIGRRKTGQEALSQTTISWTPFQESSEYIISCHPVGIDEEPLQFRVPGTSASATLTGLTRGATYNIIVEALKDQKRHKVREEVVTVGNSVDQGLGQPTADSCFDPYTVSHYAIGEEWERLSESGFKLSCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAEPGHEGSTGHSYNQYSQRYQQRTNTNVNCPIECFMPLDVQADRDDSRE

Target ORF information:

RefSeq Version	XM_023642291.1
Organism	Equus caballus(horse)
Definition	Equus caballus fibronectin 1 (FN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642291.1

ORF Insert Sequence:

ATGCTCAGGG GTCCGGGACC TGGGCTGCTG CTGCTGGTCG TCCTGTCCCT GGGGACAGCG 
GTGCCCTCCA GCGGAGCCTC GAAGAGGAAG AGGCAGGATC AGCAAATCAT TCAGCCCCAG 
TCCCCGGTGG CTGTCGGCCA AAGCAAGCCT GGTTGTTACG ACAATGGGAA ACACTATCAG 
ATAAACCAAC AATGGGAGCG CACCTACCTG GGCAATGCCT TGGTTTGTAC CTGCTACGGA 
GGGAGCAGAG GCTTTAACTG CGAGAGTAAA CCTGAGCCTG AAGAGACTTG CTTTGACAAG 
TACACCGGGA ACACTTACCG GGTCGGCGAC ACTTACGAGC GCCCTAAAGA CTCCATGATC 
TGGGACTGTA CCTGCATCGG GGCTGGGCGA GGGAGAATCA GCTGCACCAT TGCAAACCGC 
TGCCATGAAG GGGGTCAGTC CTACAAGATT GGTGACACCT GGAGGAGACC TCATGAGACT 
GGTGGTTACA TGTTAGAGTG TGTCTGTCTT GGTAATGGGA AAGGAGAATG GACCTGCAAG 
CCTATAGCTG AGAAATGTTT TGATCATGCT GCTGGGACCT CTTATGTTGT CGGGGAAACC 
TGGGAGAAGC CCTACCAAGG CTGGATGATG GTGGACTGTA CTTGTCTGGG AGAAGGCAAT 
GGGCGCATCA CCTGTACTTC TAGAAACAGA TGCAATGATC AGGACACTAG GACATCCTAT 
AGAATTGGCG ATACCTGGAG CAAGAAGGAT AATCGGGGAA ACCTGCTCCA GTGCATCTGC 
ACAGGCAACG GCAGAGGGGA GTGGAAGTGT GAAAGGCACG CGTCTCTGCA GACCACATCC 
ACCGGATCTG GCCCCTTCAC AGATGTCCGA ACAGCCATTT ACCAGCCCCA GCCTCACCCC 
CAGCCCGCTC CATACGGTCA CTGTGTCACA GACAGTGGCG TGGTTTACTC CGTGGGGATG 
CAGTGGCTGA AGACACAAGG AAATAAGCAA ATGCTTTGCA CTTGCCTGGG CAATGGAGTC 
AGCTGCCAAG AGACAGCTGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCGTGT 
GTCTTACCCT TCACCTACAA CGGCAGAACC TTCTACTCCT GCACCACGGA GGGGCGGCAG 
GATGGACACC TCTGGTGCAG CACGACTTCC AATTATGAGC AAGACCAGAA ATATTCTTTC 
TGTACAGACA GTGCTGTTTT GGTTCAGACT CGAGGTGGGA ATTCCAATGG TGCCTTGTGC 
CACTTCCCCT TCCTATACAA CAACCACAAC TACACTGACT GTACTTCTGA GGGCAGGAGA 
GACAACATGA AATGGTGTGG AACCACCCAG AACTATGACG CGGACCAGAA GTTTGGATTC 
TGCCCCATGG CTGCCCACGA GGAAATCTGT ACAACTAATG AAGGGGTGAT GTATCGCGTT 
GGAGATCAGT GGGATAAGCA ACACGACATG GGCCACATGA TGAGGTGCAC ATGTGTCGGC 
AATGGTCGTG GGGAATGGAC GTGTGTCGCC TACTCCCAGC TCCGAGATCA ATGCATTGTC 
GACGACATCA CTTATAACGT GAATGACACA TTTCATAAGC GTCACGAAGA GGGACACATG 
CTGAACTGTA CCTGCTTCGG TCAGGGCCGG GGCAGATGGA AGTGTGATCC CATTGACCAA 
TGCCAGGACT CGGAAACCCG AACGTTTTAT CAGATTGGAG ACTCCTGGGA GAAATATGTG 
CATGGTGTCA GGTACCAGTG CTATTGCTAT GGCCGTGGCA TTGGGGAGTG GCATTGCCAA 
CCTTTGCAGA CCTACCCGGG CACCACTGGT CCTGTCCAAG TAATCATCAC AGAGACCCCC 
AGCCAGCCCA ACTCACACCC CATCCAGTGG AACGCACCGG AGCCATCTCA CATCTCTAAG 
TACATCCTCA GATGGAAACC CAAAAATGCT CCAGGCCGTT GGAAGGAAGC CACCATTCCA 
GGCCACTTGA ACTCGTACAC TATCAAAGGC CTGAGGCCAG GTGTGGTATA TGAGGGACAG 
CTCATCAGTG TCCAGCACTA CGGCCACAAA GAGGTGACAC GCTTTGACTT CACCACCACC 
AGCACAAGCC CAGCAGTGAC CAGCAACACT GTGACTGGAG AGACAACACC GTTTTCCCCC 
GTTGTGGCCA CTTCTGAATC TGTGACTGAA ATCACCGCCA GCAGCTTCGT GGTCTCTTGG 
GTCTCGGCTT CAGACACCGT GTCAGGATTC CGTGTGGAAT ATGAGCTGAG TGAGGAGGGA 
GATGAACCAC AGTACCTCGA TCTTCCAAGC ACGGCCACTT CTGTAAACAT CCCTGACCTG 
CTTCCTGGCC GAAAATACAT CGTAAATGTC TATCAGATTT CTGAAGAAGG AGAGCAGAGT 
CTGATCCTGT CTACTTCGCA GACAACAGCG CCTGATGCCC CTCCTGATCC TACTGTGGAC 
CAAGTTGATG ACACCTCGAT TGTTGTTCGC TGGAGCAGAC CTCAGGCACC CATCACAGGA 
TACAGAATCG TGTATTCACC ATCAGTAGAA GGGAGTAGCA CAGAACTCAC CCTTCCTGAA 
ACTGCCAACT CCGTCACCCT CAGCGACTTG CAACCTGGGG TTCAGTATAA CATCACCATC 
TATGCTGTGG AAGAGAATCA AGAAAGTACT CCTGTTTTCA TCCAACAGGA AACTACTGGC 
GTCCCACGTG CAGATAAAGT TCCGCCTCCC CGGGACCTGC AGTTTGTGGA GGTGACAGAC 
GTGAAGATCA CCATCATGTG GACACCCCCT GAGAGCGCGG TGACCGGCTA CCGTGTGGAC 
GTGCTCCCCG TCAACCTGCC TGGGGAGCAC GGCCAGAGGC TGCCCATCAG CAGGAACACG 
TTTGCAGAAG TCACAGGGCT GTCCCCTGGG GTCACCTATC ACTTCAAAAT CTTCGCCGTG 
AACCACGGGA GGGAAAGCAA GCCTCTGACA GGAGAACAGA CAACCAAATT GGATGCTCCC 
ACTAACCTCC GGTTTATCAA TGAAACTGAG TCGACTGTCA TAGTGACCTG GACACCCCCT 
CGGGCCCGGA TAGCCGGGTA TCGGCTGACC GTGGGCCTGA CCCGGGGAGG CCAGCCCAAG 
CAGTACAACG TGGGGCCCTC TGCCTCTCAG TACCCACTGA GGAGTCTGCA GCCTGGGTCT 
GAGTACACCG TCACCCTCGT GGCCGTCAAA GGCAACCAGC AGAGCCCCAA AGCCACTGGA 
GTCTTTACTA CTCTGCAGTC TCCAGGCTCC ATTCCACCTT ACAACACAGA GGTGACTGAG 
ACCACCATTG TGATTACATG GACGCCTGCT CCAAGGATTG GTTTTAAGCT GGGTGTGCGA 
CCAAGCCAGG GAGGGGAAGC ACCGCGAGAA GTGACTTCAG ACTCAGGAAG CATCGTTGTG 
TCTGGCCTGA CCCCAGGCGT GGAATACGTT TACACCATCT CAGTCCTGAG AGATGGGCAG 
GAGAGAGATG CGCCAATCGT GAAGAAAGTG GTGACACCAT TGTCTCCCCC AACAAACTTG 
CACCTGGAGG CAAACCCCGA CACTGGAGTG CTTACGGTCT CCTGGGAGAG GAGCACCACC 
CCAGACATTA CTGGTTATAG AATCACCACC ACGCCTACAA GTGGCCAACA AGGCTATTCT 
CTGGAAGAAG TGGTTCATGC AGATCAGAGT TCTTGCACTT TTGAAAACCT GAGTCCTGGC 
CTGGAGTACA ATGTCAGTGT TTACACTGTC AAAGATGACA AGGAAAGTGT CCCTATCTCT 
GATACCATCA TCCCAGCCGT CCCTCCTCCC ACTGACCTGC GATTCACCAA TGTTGGTCCA 
GACACTATGC GTGTCACCTG GGCCCCACCT CCATCTATTG AATTGACCAA CCTCCTGGTG 
CGCTACTCAC CCGTGAAAAA TGAGGAAGAT GTTGCGGAAT TGTCAATTTC TCCTTCAGAC 
AATGCAGTGG TCTTAACAAA TCTCCTGCCT GGGACAGAAT ACTTAGTCAG TGTCTCCAGC 
GTTTACGAAC AACACGAGAG CACACCTCTT AGAGGAAGAC AGAAGACAGG TCTTGATTCC 
CCAACTGGCA TTGACTTTTC TGATATCACT GCCAACTCTT TCACGGTCCA TTGGATTGCT 
CCTCGAGCCA CCATCACTGG CTACAGGATT CGCCATCATC CTGAGCACAC TGGTGGAAGA 
CCACGAGAGG ATCGAGTGCC TCCCTCTCGG AATTCTATCA CCCTCACCAA CCTCAATCCA 
GGCACAGAAT ATGTGGTCAG CATCGTTGCT CTTAATGGCA GAGAGGAAAG TCCTCCCTTG 
GTTGGCCAAC AATCAACAGT TTCTGACGTT CCAAGGGACC TGGAAGTTAT TGCCACAACC 
CCCACCAGCA TCTTGATCAG CTGGGACGCT CCTGCCGTCA CAGTGAGATA TTACAGGATC 
ACCTATGGAG AAACAGGAGG AAATAGCCCT GTCCAGGAGT TCACTGTGCC TGGCAGCAAG 
TCTACAGCTA CCATCAGTGG CCTTAAACCT GGAGTAGACT ACACCATCAC CGTTTATGCT 
GTCACTGGCC GCGGGGACAG CCCAGCCAGC AGCAAGCCAA TTTCCATTGA TTACCGAACA 
GAAATTGACA AACCATCCCA GATGCAAGTG ACTGATGTTC AGGACAACAG CATTAGTGTC 
AGGTGGCTGC CTTCAAGTTC CCCTGTTACT GGCTACAGAG TGACCACTAC TCCCAAAAAT 
GGCCCAGGAC AATCAAAAAC TAAAACTGCA GGTCCAGATC AAACAGAAAT GACCATCGAA 
GGCTTACAAC CCACAGTGGA GTATGTGGTT AGTGTCTATG CTCAGAATCA AAACGGAGAG 
AGTCAGCCTC TGGTTCAGAC AGCAGTAACC ACCATTCCTG CACCAACTGA ACTCAAGTTC 
ACTCAGGTTA CACCAACAAG CCTGACCGCC CAGTGGACAG CACCCAATGT TCAGCTCACT 
GGGTACCGCG TGCGGGTGAC CCCCAAGGAA AAGACTGGAC CAATGAAAGA AATTAACCTT 
GCTCCCGATA GCACATCTGT GGTCGTATCA GGACTCATGG TGGCCACCAA GTATGAAGTG 
AGTGTCTATG CTCTTAAGGA CACTTTGACA AGCAGACCGG CTCAGGGAAT TGTCACGACC 
CTGGAGAATG TCAGCCCTCC AAGAAGGGCC CGCGTGACAG ATGCTACTGA GACTACCATC 
ACCATTAGCT GGAGAACCAA GACTGAGACC ATCACTGGCT TCCAGGTTGA TGCCGTCCCA 
GCAAATGGCC AGCCTCCAAT CCAGAGAACC ATCAAGCCAG ATGTCAGAAG CTACACCATC 
ACAGGTTTGC AACCCGGCAC TGACTACAAG ATCTACTTGT ACACCCTGAA TGACAATGCC 
CGGAGCTCCC CTGTGATCAT CGACGCCTCT ACTGCCATTG ATGCACCATC CAACCTGCAT 
TTCCTGGCCA CCACGCCCAA CTCCTTGCTG ATATCATGGC AGCCACCCCG TGCCAGGATT 
ACTGGTTACA TCATCAAGTA TGAGAAGCCT GGGTCCCCTC CCAGAGAAGT GGTCCCTCGG 
CCCCACCCTG GTGTCACAGA GGCTACTATT ACTGGTCTGG AACCAGGAAC CGAGTACACT 
ATTCAAGTCA TTGCCATCAA GAACAACCAG AAGAGCGAGC CTCTGATCGG GAGGAGAAAG 
ACAGGACAAG AGGCTCTCTC TCAGACAACC ATCTCTTGGA CCCCATTCCA GGAAAGCTCT 
GAGTATATCA TTTCATGTCA TCCAGTTGGC ATTGATGAAG AACCCTTACA GTTCCGAGTT 
CCTGGAACCT CTGCTAGTGC CACCCTGACG GGTCTTACCA GAGGGGCCAC CTACAACATC 
ATAGTGGAGG CACTGAAAGA CCAGAAGAGG CACAAGGTTC GGGAGGAGGT CGTTACTGTG 
GGCAACTCTG TTGACCAAGG CCTAGGCCAA CCTACGGCTG ACTCGTGCTT CGACCCCTAC 
ACCGTCTCCC ATTACGCCAT CGGAGAGGAG TGGGAGCGAC TGTCTGAATC TGGCTTTAAA 
CTCTCCTGCC AGTGCTTAGG GTTTGGCAGT GGCCATTTCA GATGCGATTC GTCTAAGTGG 
TGCCATGACA ATGGTGTGAA CTACAAGATT GGAGAGAAGT GGGATCGTCA GGGAGAAAAT 
GGCCAGATGA TGAGCTGCAC ATGTCTTGGA AATGGAAAAG GAGAATTCAA GTGTGACCCT 
CATGAAGCAA CATGTTATGA TGACGGGAAG ACGTACCACG TAGGAGAACA GTGGCAGAAG 
GAGTATCTTG GTGCTATTTG CTCCTGCACA TGCTTTGGAG GCCAGCGGGG CTGGCGCTGT 
GACAACTGCC GCAGACCTGG GGCTGAACCC GGTCACGAAG GCTCCACTGG GCACTCCTAC 
AACCAGTATT CACAGAGATA CCAGCAGAGA ACCAACACCA ATGTCAACTG CCCAATTGAG 
TGCTTCATGC CTTTAGATGT ACAGGCTGAC AGAGATGATT CCCGAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	FN1
Entrez Gene ID	100034189
Full Name	fibronectin 1
Gene Type	protein-coding
Organism	Equus caballus(horse)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

FN1 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

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