Dsim\GD22090 cDNA ORF clone, Drosophila simulans

Gene
Clones
gRNAs

Summary

Gene Symbol	Dsim\GD22090
Entrez Gene ID	6732145
Full Name	GD22090 gene product from transcript GD22090-RB
Synonyms	Dsim_GD22090,GD22090,dsim_GLEANR_5849
Gene Type	protein-coding
Organism	Drosophila simulans

mRNA and Protein(s)

mRNA	Protein	Name
XM_016178334.1	XP_016024829.1	uncharacterized protein, isoform D
XM_016178337.1	XP_016024832.1	uncharacterized protein, isoform G
XM_016178335.1	XP_016024830.1	uncharacterized protein, isoform E
XM_016178336.1	XP_016024831.1	uncharacterized protein, isoform F
XM_016178332.1	XP_016024827.1	uncharacterized protein, isoform B
XM_016178333.1	XP_016024828.1	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Hu TT, Eisen MB, Thornton KR, Andolfatto P
Genome research23(1)89-98(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dsim\GD22090 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dsim\GD22090 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODg18244	XM_016178334.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant D (Dsim\GD22090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODg18243	XM_016178333.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant C (Dsim\GD22090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODg18245	XM_016178335.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant E (Dsim\GD22090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODg18246	XM_016178336.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant F (Dsim\GD22090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODg18242	XM_016178332.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant B (Dsim\GD22090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODg18247	XM_016178337.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant G (Dsim\GD22090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dsim\GD22090 ( XM_016178334.1 ) cDNA ORF clone, Drosophila simulans -> XP_016024829.1 Drosophila simulans uncharacterized protein, transcript variant D (Dsim\GD22090), mRNA.

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CloneID	ODg18244
Clone ID Related Accession (Same CDS sequence)	XM_016178334.1
Accession Version	XM_016178334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGCGGCA CTGTCGCTTA AAGCATTCCG GGGATATTCG 
GATCGAGACC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016024829.1
CDS	1216..4770
Translation	MDLPSMVQRSGDTLIVRSVVSGNQLYTEQGGHQPASGNTGHMTSNSTSNTPAAQAGSSLLERHVERFRLQQLLQQQRQAAAAVAVVNSVQQQQQQQPQQVAIGMDAKEEGLPQCKIKRNYSCNHCAYFTQNPRYHLTHLRDVHGEKIVINKCKLCLYASRHFQKLVRHMKMVHGCTDGIPSGHGQARGKRGMSREARKRRLEESVGVMGGQSLTVTVPDVPTLEQVKRELLLQEEKLQRDIQAFNQRQREEQQREQQRELELVATSAYERQMQVLRDYERQSPAEPPTPSPTSGSATPPSNGEEPQNRLLKCSACEFTTLYRTQLRAHELAEHGKTKFFRCDKCSYVTHIKARFSKHVKYHSMPMIKCVTCDFRTPYKWNLDRHMKNHGGAGAFKCAACDFTADIKQSLTVHEMNHHVPPVGNAGSIWPRRQNKVGASEMCEDFLSDSAELEDQYNNNNVDDELDGVEDADDAMSGGEEQPMHLHHYGKRSKYDDEEEPTDLSQKGGCSSDTSSVGTPTPRAQRTMPNLIPISKGAKDVLNLSKETNASRSSLTEIASMFFNEKQISEMLDKSDVPQLSPATTVASQNSTRSQMTKKSSFMDKLKTGAQHENLVCQCGHVAKCLSESIIHGKSCHASAVIIDEDDAGLHEDDADDRLEIDEDDEDHHSHSALNLSVTGSTRCQHCRHRCKSSTDLMHHLKQCVEAIRCANEMYDSNSGESGDRRPDSQSLQQQAAVQQQRVCIWNKATKEIVAAAAASMQQEKNNHNLVKSPTGSQAASNEENSYYGVETAPGYGEVTKKMTPEEEAANSSLKKVYKCPHCSFWASTASRFHVHIVGHLNKKPFECSLCSYRSNWRWDITKHIRLKTIRDPSHKTAKVLMNDETGRRNYTKYNKYITLMKVTEEDGDPKLMKSGEMTPNQVASLAFLKDYAKVGSVSGQDITLEPVLPNKPNVLDDAHLADNLIRIPLLATMMNAAMAQQQHQQQQQKEHQSTMITPSVTISPVKRSNQASVMQGKPSDDLITEVHQEGNEKRTVYRCRKCNFGHQNRDAVLAHVKIHYQDASYPKSNSGNSSTSPLQVSVGGSPQFYMNKVFAAMCLSQTQSQSSPNSGSAGTSPAISAGLLQRAIQEAQHSPTPNASALSGLALALAGGKPQANTTKASAASILEHAALSLKAFRGYSDRDP

Target ORF information:

RefSeq Version	XM_016178334.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant D (Dsim\GD22090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016178334.1

ORF Insert Sequence:

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGCGGCA CTGTCGCTTA AAGCATTCCG GGGATATTCG 
GATCGAGACC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD22090 ( XM_016178333.1 ) cDNA ORF clone, Drosophila simulans -> XP_016024828.1 Drosophila simulans uncharacterized protein, transcript variant C (Dsim\GD22090), mRNA.

Price & Availability↑

CloneID	ODg18243
Clone ID Related Accession (Same CDS sequence)	XM_016178333.1
Accession Version	XM_016178333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGAT GACACCGGAG 
GAAGAGGCAG CCAACTCCTC GCTGAAGAAG GTGTACAAGT GTCCGCACTG CAGCTTCTGG 
GCCTCCACGG CGTCCCGCTT CCACGTCCAC ATCGTGGGCC ATCTGAACAA GAAGCCCTTT 
GAGTGCTCCC TCTGCTCGTA CCGCTCCAAC TGGCGCTGGG ACATCACCAA GCACATCCGA 
TTGAAGACGA TCCGCGATCC CTCGCACAAG ACGGCCAAGG TTCTGATGAA CGACGAGACG 
GGCCGTAGAA ACTACACCAA GTACAACAAA TACATTACCC TGATGAAGGT AACCGAGGAG 
GATGGTGATC CCAAGCTGAT GAAGTCCGGC GAGATGACAC CGAACCAGGT GGCCTCGTTG 
GCCTTCCTCA AGGACTATGC CAAGGTCGGC TCGGTTTCCG GGCAGGATAT CACATTGGAA 
CCAGTGCTTC CCAACAAACC CAATGTACTG GACGATGCCC ATTTGGCGGA CAACCTAATA 
CGGATTCCCT TGCTGGCCAC CATGATGAAT GCCGCTATGG CGCAACAGCA ACACCAGCAG 
CAGCAACAGA AGGAGCACCA GTCAACGATG ATCACGCCCT CGGTGACCAT TTCCCCGGTC 
AAGCGATCCA ATCAGGCATC CGTGATGCAG GGCAAGCCCA GCGACGACCT GATCACCGAG 
GTCCACCAGG AGGGCAATGA GAAGAGGACC GTCTATCGGT GCCGCAAGTG TAACTTCGGG 
CACCAGAATC GCGATGCTGT GCTGGCGCAC GTGAAGATTC ACTACCAGGA CGCCAGCTAC 
CCGAAGTCCA ACTCTGGGAA CTCCAGCACT TCGCCACTGC AGGTATCCGT GGGCGGTAGT 
CCACAATTCT ACATGAACAA GGTCTTTGCG GCCATGTGCC TGTCGCAGAC GCAGTCACAG 
TCGTCGCCCA ACTCCGGCTC CGCCGGAACG AGTCCGGCCA TCTCGGCCGG ACTGCTGCAG 
CGGGCCATCC AGGAGGCCCA GCACTCGCCC ACTCCGAACG CCAGCGCTCT GAGCGGACTT 
GCTTTGGCGT TGGCGGGCGG AAAGCCACAA GCCAATACGA CGAAAGCCAG TGCCGCCTCC 
ATTTTAGAGC ACGGTGAGCA GAAAGCGAGT CAAAACGAGG CAAGTGCCGA CAGTCTGACG 
TCGCCGAACA CCACCACCAC GAACTCAACC ACTAGTAGAA CCACCAAAGA CAACACCGCC 
AGATCGCTGC AGGATCTGCT CACCTCACCG CGCAGTGCCA GAAATGGAAA TCATACTTCT 
AACGCTAACA GTAACTCGGT TGTGGGAAAT GGACTCCGGC TGGATGCCGT CTACGTCGCC 
TCATCCACCA ACAATACCAC AACACCACCA CCACCTCCCA CCAACTCAGT CAGCAAGCCC 
AATGCATCGC CTTTGCCACG GCACTGTCGC TTAAAGCATT CCGGGGATAT TCGGATCGAG 
ACCCTTGAGC GAGGAGCCAG TGTCTCGGCG AACGCACCAC CCATCTACCG ACCCCTGGGA 
GCAGGTGCCA GCAACGAATC CCAGTCCCAC ATCCACAATC CGTCGAATCC CATCACCAGC 
AACATCCTAA AGTCGGGCAG CAACACGAAT GCCAACAGCA ACATCAACAG CAATAGTGCC 
CAGCCCCTGC TGCTGACCAC CAAGCAGCTG GAGCAGATCT TCCACACACC GCTCTCCGCC 
AGCGCCGCTG CAGTGGTGAA TGCGGCCAAC ACCCAGCAGG ATATCCAAGC GGCGGCGGCC 
TATTGGGCAG CAGCCTGCAA AGCAGTGGTG GCCAACAGCA GCGCCGAGGA GTTGCTGCAG 
TTGCAGCAGA ACGACCAGAT CGAGATCACC CGCCTGTCGA CCACGGCGGA GCACTCCATC 
GGATCCAGCG CCGGCGGCAA CAATACAAAG AGCAAGCAGC AGAAGTGTCC CGTCTGTCCG 
TACATCTCGG AGAGCAAATC CCAGATGAAC TACCACGTGT CGCTCCACAA GCCCACGCAG 
TACGAGTGCC GGCTGTGCAC ATTTGTGTGT GCCAAGAAAC AGCATCTGAG CAGCCACATG 
AGAACGGTGC ACCAGCAGCA GATGGTTGCA TCGGGAGCAG GTGCAGCCGC AGGAGCAGCC 
GGAGGAGTAG GAGGAGGCGG AGCTTCCTCG CTGGGTCTGG ACTTCAGTGT GGCCCTGCAG 
TTGGCCGCAG CTGCCAAGCA GGTGCAACAA CTGCCCGCCT CAACACCACT CTCCATCGAT 
TTGTCCCAGA TCCAGATGGA TGCCGCCTCC GATGCGGAGC TCAATCCACA GCAACTGCCG 
CCGGAGTACC AATACAAGCT GATCAGCTAC TGTCCCAGGT GTCCGGCTCG CTTTGCCCAG 
AAGCACAACG ACGAGCGCAA TGCCAAGCAA GAGCTGGAGC AGCATTTGCT GGCACACAGC 
GACCAGGAGC CGGAGGATCA GGATCCGAGC TATGTCTGCG CCTACTGTGA GTATCGTACA 
TCGGAAGAGA CCCTGCTGCA ACTGCACCGT GCGGTGCACA TGTCCCACTA CCAGGAGAAG 
TGCCAGGAGC TGTACCGGAA CTGCAAGGAG GATGTCGAGT ATCCGGCACC CAAGTTGCTG 
CAGCTGACCG GTCCCGAGAC CATTTGGGTG GTAGACAATG AGCTGAGCTT GCAGCTGCTG 
CAGCAAACTA CCGGAGGAGC AGTTAGTACC TCCACCGGTG GGTTTGACAA CCAGAACTCT 
CTGCTCAAGA AGCAGCTGGA ATCGGGCGGT GGCCAGATGG TCGCACTTCC ACGGGAAGCA 
TCCGTGACCA AAAATGAGGC GGAAGGCCCC GAGGAGGACG AGGAGCGACG TAGCAGCTCC 
ACGCCGTCGA CCAGCGCTTC GGTGGCCACC AGTGATTTGG CCGCAGATGG CAGCAGTGAT 
GCAGGATCCG TTGACATGCC CCAGTCCATT CCGGCACCGG AACGCTGTCT CCATTGTCCA 
TTCGAAACGC AGCATCACGA GGAACTGATG CAGCATCTCC AGAAGCACGC CTGCGTGAAT 
CCTCCACCTC CCAACTACCA GAAATGTGCC CACTGTGATT ACAATGCCAA TGAGGAGGCG 
GAAATGGAGG AGCACACGGC GCTGCATTTC AATGCCAGTG AGAAGCTCAA GTCCGTGGAG 
TTCTTCACCT GCTACGATCA GCTGGAGATC AGCGTGGAGC GGGAACCGGA GGAAACCGCT 
GACTCAAAGG CGGAGCAGAC CGAGCACAAC AACAACCAGG ACAATGTGAT GAACGCCAAT 
GTGCAGCAGC AGCAGCAGCC GAGCAGCAAC AGCCCAGATG AACAGCCAGC TGACGAGGGG 
ATGGAGGAAT CGTCGGCCAA GAAGTCCAGC ACCAAGTTGA TTCTGTACAA GAACGATGGC 
GGTTTGAGTG TAAAGCTCTC CCAGGAAGAG GGCGCCAATT CAGAGTTCCA GAAGAGCGAG 
AATATCAGCG ATCGCCTGCG CAGAAGGATC CTCCGGGGAA GTGCCAACCA GACGGAGGAC 
GCCACTTCCA CCTCCGGCCA GGAGCGACCC GCAACACCGG ACAAAATGAT ACTGGTCAAT 
GCCCAGACGG GCAAAGTCAT TTCCAGAAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016024828.1
CDS	1216..7188
Translation	MDLPSMVQRSGDTLIVRSVVSGNQLYTEQGGHQPASGNTGHMTSNSTSNTPAAQAGSSLLERHVERFRLQQLLQQQRQAAAAVAVVNSVQQQQQQQPQQVAIGMDAKEEGLPQCKIKRNYSCNHCAYFTQNPRYHLTHLRDVHGEKIVINKCKLCLYASRHFQKLVRHMKMVHGCTDGIPSGHGQARGKRGMSREARKRRLEESVGVMGGQSLTVTVPDVPTLEQVKRELLLQEEKLQRDIQAFNQRQREEQQREQQRELELVATSAYERQMQVLRDYERQSPAEPPTPSPTSGSATPPSNGEEPQNRLLKCSACEFTTLYRTQLRAHELAEHGKTKFFRCDKCSYVTHIKARFSKHVKYHSMPMIKCVTCDFRTPYKWNLDRHMKNHGGAGAFKCAACDFTADIKQSLTVHEMNHHVPPVGNAGSIWPRRQNKVGASEMCEDFLSDSAELEDQYNNNNVDDELDGVEDADDAMSGGEEQPMHLHHYGKRSKYDDEEEPTDLSQKGGCSSDTSSVGTPTPRAQRTMPNLIPISKGAKDVLNLSKETNASRSSLTEIASMFFNEKQISEMLDKSDVPQLSPATTVASQNSTRSQMTKKSSFMDKLKTGAQHENLVCQCGHVAKCLSESIIHGKSCHASAVIIDEDDAGLHEDDADDRLEIDEDDEDHHSHSALNLSVTGSTRCQHCRHRCKSSTDLMHHLKQCVEAIRCANEMYDSNSGESGDRRPDSQSLQQQAAVQQQRVCIWNKATKEIVAAAAASMQQEKNNHNLVKSPTGSQAASNEENSYYGVETAPGYGEMTPEEEAANSSLKKVYKCPHCSFWASTASRFHVHIVGHLNKKPFECSLCSYRSNWRWDITKHIRLKTIRDPSHKTAKVLMNDETGRRNYTKYNKYITLMKVTEEDGDPKLMKSGEMTPNQVASLAFLKDYAKVGSVSGQDITLEPVLPNKPNVLDDAHLADNLIRIPLLATMMNAAMAQQQHQQQQQKEHQSTMITPSVTISPVKRSNQASVMQGKPSDDLITEVHQEGNEKRTVYRCRKCNFGHQNRDAVLAHVKIHYQDASYPKSNSGNSSTSPLQVSVGGSPQFYMNKVFAAMCLSQTQSQSSPNSGSAGTSPAISAGLLQRAIQEAQHSPTPNASALSGLALALAGGKPQANTTKASAASILEHGEQKASQNEASADSLTSPNTTTTNSTTSRTTKDNTARSLQDLLTSPRSARNGNHTSNANSNSVVGNGLRLDAVYVASSTNNTTTPPPPPTNSVSKPNASPLPRHCRLKHSGDIRIETLERGASVSANAPPIYRPLGAGASNESQSHIHNPSNPITSNILKSGSNTNANSNINSNSAQPLLLTTKQLEQIFHTPLSASAAAVVNAANTQQDIQAAAAYWAAACKAVVANSSAEELLQLQQNDQIEITRLSTTAEHSIGSSAGGNNTKSKQQKCPVCPYISESKSQMNYHVSLHKPTQYECRLCTFVCAKKQHLSSHMRTVHQQQMVASGAGAAAGAAGGVGGGGASSLGLDFSVALQLAAAAKQVQQLPASTPLSIDLSQIQMDAASDAELNPQQLPPEYQYKLISYCPRCPARFAQKHNDERNAKQELEQHLLAHSDQEPEDQDPSYVCAYCEYRTSEETLLQLHRAVHMSHYQEKCQELYRNCKEDVEYPAPKLLQLTGPETIWVVDNELSLQLLQQTTGGAVSTSTGGFDNQNSLLKKQLESGGGQMVALPREASVTKNEAEGPEEDEERRSSSTPSTSASVATSDLAADGSSDAGSVDMPQSIPAPERCLHCPFETQHHEELMQHLQKHACVNPPPPNYQKCAHCDYNANEEAEMEEHTALHFNASEKLKSVEFFTCYDQLEISVEREPEETADSKAEQTEHNNNQDNVMNANVQQQQQPSSNSPDEQPADEGMEESSAKKSSTKLILYKNDGGLSVKLSQEEGANSEFQKSENISDRLRRRILRGSANQTEDATSTSGQERPATPDKMILVNAQTGKVISRK

Target ORF information:

RefSeq Version	XM_016178333.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant C (Dsim\GD22090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016178333.1

ORF Insert Sequence:

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGAT GACACCGGAG 
GAAGAGGCAG CCAACTCCTC GCTGAAGAAG GTGTACAAGT GTCCGCACTG CAGCTTCTGG 
GCCTCCACGG CGTCCCGCTT CCACGTCCAC ATCGTGGGCC ATCTGAACAA GAAGCCCTTT 
GAGTGCTCCC TCTGCTCGTA CCGCTCCAAC TGGCGCTGGG ACATCACCAA GCACATCCGA 
TTGAAGACGA TCCGCGATCC CTCGCACAAG ACGGCCAAGG TTCTGATGAA CGACGAGACG 
GGCCGTAGAA ACTACACCAA GTACAACAAA TACATTACCC TGATGAAGGT AACCGAGGAG 
GATGGTGATC CCAAGCTGAT GAAGTCCGGC GAGATGACAC CGAACCAGGT GGCCTCGTTG 
GCCTTCCTCA AGGACTATGC CAAGGTCGGC TCGGTTTCCG GGCAGGATAT CACATTGGAA 
CCAGTGCTTC CCAACAAACC CAATGTACTG GACGATGCCC ATTTGGCGGA CAACCTAATA 
CGGATTCCCT TGCTGGCCAC CATGATGAAT GCCGCTATGG CGCAACAGCA ACACCAGCAG 
CAGCAACAGA AGGAGCACCA GTCAACGATG ATCACGCCCT CGGTGACCAT TTCCCCGGTC 
AAGCGATCCA ATCAGGCATC CGTGATGCAG GGCAAGCCCA GCGACGACCT GATCACCGAG 
GTCCACCAGG AGGGCAATGA GAAGAGGACC GTCTATCGGT GCCGCAAGTG TAACTTCGGG 
CACCAGAATC GCGATGCTGT GCTGGCGCAC GTGAAGATTC ACTACCAGGA CGCCAGCTAC 
CCGAAGTCCA ACTCTGGGAA CTCCAGCACT TCGCCACTGC AGGTATCCGT GGGCGGTAGT 
CCACAATTCT ACATGAACAA GGTCTTTGCG GCCATGTGCC TGTCGCAGAC GCAGTCACAG 
TCGTCGCCCA ACTCCGGCTC CGCCGGAACG AGTCCGGCCA TCTCGGCCGG ACTGCTGCAG 
CGGGCCATCC AGGAGGCCCA GCACTCGCCC ACTCCGAACG CCAGCGCTCT GAGCGGACTT 
GCTTTGGCGT TGGCGGGCGG AAAGCCACAA GCCAATACGA CGAAAGCCAG TGCCGCCTCC 
ATTTTAGAGC ACGGTGAGCA GAAAGCGAGT CAAAACGAGG CAAGTGCCGA CAGTCTGACG 
TCGCCGAACA CCACCACCAC GAACTCAACC ACTAGTAGAA CCACCAAAGA CAACACCGCC 
AGATCGCTGC AGGATCTGCT CACCTCACCG CGCAGTGCCA GAAATGGAAA TCATACTTCT 
AACGCTAACA GTAACTCGGT TGTGGGAAAT GGACTCCGGC TGGATGCCGT CTACGTCGCC 
TCATCCACCA ACAATACCAC AACACCACCA CCACCTCCCA CCAACTCAGT CAGCAAGCCC 
AATGCATCGC CTTTGCCACG GCACTGTCGC TTAAAGCATT CCGGGGATAT TCGGATCGAG 
ACCCTTGAGC GAGGAGCCAG TGTCTCGGCG AACGCACCAC CCATCTACCG ACCCCTGGGA 
GCAGGTGCCA GCAACGAATC CCAGTCCCAC ATCCACAATC CGTCGAATCC CATCACCAGC 
AACATCCTAA AGTCGGGCAG CAACACGAAT GCCAACAGCA ACATCAACAG CAATAGTGCC 
CAGCCCCTGC TGCTGACCAC CAAGCAGCTG GAGCAGATCT TCCACACACC GCTCTCCGCC 
AGCGCCGCTG CAGTGGTGAA TGCGGCCAAC ACCCAGCAGG ATATCCAAGC GGCGGCGGCC 
TATTGGGCAG CAGCCTGCAA AGCAGTGGTG GCCAACAGCA GCGCCGAGGA GTTGCTGCAG 
TTGCAGCAGA ACGACCAGAT CGAGATCACC CGCCTGTCGA CCACGGCGGA GCACTCCATC 
GGATCCAGCG CCGGCGGCAA CAATACAAAG AGCAAGCAGC AGAAGTGTCC CGTCTGTCCG 
TACATCTCGG AGAGCAAATC CCAGATGAAC TACCACGTGT CGCTCCACAA GCCCACGCAG 
TACGAGTGCC GGCTGTGCAC ATTTGTGTGT GCCAAGAAAC AGCATCTGAG CAGCCACATG 
AGAACGGTGC ACCAGCAGCA GATGGTTGCA TCGGGAGCAG GTGCAGCCGC AGGAGCAGCC 
GGAGGAGTAG GAGGAGGCGG AGCTTCCTCG CTGGGTCTGG ACTTCAGTGT GGCCCTGCAG 
TTGGCCGCAG CTGCCAAGCA GGTGCAACAA CTGCCCGCCT CAACACCACT CTCCATCGAT 
TTGTCCCAGA TCCAGATGGA TGCCGCCTCC GATGCGGAGC TCAATCCACA GCAACTGCCG 
CCGGAGTACC AATACAAGCT GATCAGCTAC TGTCCCAGGT GTCCGGCTCG CTTTGCCCAG 
AAGCACAACG ACGAGCGCAA TGCCAAGCAA GAGCTGGAGC AGCATTTGCT GGCACACAGC 
GACCAGGAGC CGGAGGATCA GGATCCGAGC TATGTCTGCG CCTACTGTGA GTATCGTACA 
TCGGAAGAGA CCCTGCTGCA ACTGCACCGT GCGGTGCACA TGTCCCACTA CCAGGAGAAG 
TGCCAGGAGC TGTACCGGAA CTGCAAGGAG GATGTCGAGT ATCCGGCACC CAAGTTGCTG 
CAGCTGACCG GTCCCGAGAC CATTTGGGTG GTAGACAATG AGCTGAGCTT GCAGCTGCTG 
CAGCAAACTA CCGGAGGAGC AGTTAGTACC TCCACCGGTG GGTTTGACAA CCAGAACTCT 
CTGCTCAAGA AGCAGCTGGA ATCGGGCGGT GGCCAGATGG TCGCACTTCC ACGGGAAGCA 
TCCGTGACCA AAAATGAGGC GGAAGGCCCC GAGGAGGACG AGGAGCGACG TAGCAGCTCC 
ACGCCGTCGA CCAGCGCTTC GGTGGCCACC AGTGATTTGG CCGCAGATGG CAGCAGTGAT 
GCAGGATCCG TTGACATGCC CCAGTCCATT CCGGCACCGG AACGCTGTCT CCATTGTCCA 
TTCGAAACGC AGCATCACGA GGAACTGATG CAGCATCTCC AGAAGCACGC CTGCGTGAAT 
CCTCCACCTC CCAACTACCA GAAATGTGCC CACTGTGATT ACAATGCCAA TGAGGAGGCG 
GAAATGGAGG AGCACACGGC GCTGCATTTC AATGCCAGTG AGAAGCTCAA GTCCGTGGAG 
TTCTTCACCT GCTACGATCA GCTGGAGATC AGCGTGGAGC GGGAACCGGA GGAAACCGCT 
GACTCAAAGG CGGAGCAGAC CGAGCACAAC AACAACCAGG ACAATGTGAT GAACGCCAAT 
GTGCAGCAGC AGCAGCAGCC GAGCAGCAAC AGCCCAGATG AACAGCCAGC TGACGAGGGG 
ATGGAGGAAT CGTCGGCCAA GAAGTCCAGC ACCAAGTTGA TTCTGTACAA GAACGATGGC 
GGTTTGAGTG TAAAGCTCTC CCAGGAAGAG GGCGCCAATT CAGAGTTCCA GAAGAGCGAG 
AATATCAGCG ATCGCCTGCG CAGAAGGATC CTCCGGGGAA GTGCCAACCA GACGGAGGAC 
GCCACTTCCA CCTCCGGCCA GGAGCGACCC GCAACACCGG ACAAAATGAT ACTGGTCAAT 
GCCCAGACGG GCAAAGTCAT TTCCAGAAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD22090 ( XM_016178335.1 ) cDNA ORF clone, Drosophila simulans -> XP_016024830.1 Drosophila simulans uncharacterized protein, transcript variant E (Dsim\GD22090), mRNA.

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CloneID	ODg18245
Clone ID Related Accession (Same CDS sequence)	XM_016178335.1
Accession Version	XM_016178335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGGTGAG CAGAAAGCGA GTCAAAACGA GGCAAGTGCC 
GACAGTCTGA CGTCGCCGAA CACCACCACC ACGAACTCAA CCACTAGTAG AACCACCAAA 
GACAACACCG CCAGATCGCT GCAGGATCTG CTCACCTCAC CGCGCAGTGC CAGAAATGGA 
AATCATACTT CTAACGCTAA CAGTAACTCG GTTGTGGGAA ATGGACTCCG GCTGGATGCC 
GTCTACGTCG CCTCATCCAC CAACAATACC ACAACACCAC CACCACCTCC CACCAACTCA 
GTCAGCAAGC CCAATGCATC GCCTTTGCCA CGGCACTGTC GCTTAAAGCA TTCCGGGGAT 
ATTCGGATCG AGACCCTTGA GCGAGGAGCC AGTGTCTCGG CGAACGCACC ACCCATCTAC 
CGACCCCTGG GAGCAGGTGC CAGCAACGAA TCCCAGTCCC ACATCCACAA TCCGTCGAAT 
CCCATCACCA GCAACATCCT AAAGTCGGGC AGCAACACGA ATGCCAACAG CAACATCAAC 
AGCAATAGTG CCCAGCCCCT GCTGCTGACC ACCAAGCAGC TGGAGCAGAT CTTCCACACA 
CCGCTCTCCG CCAGCGCCGC TGCAGTGGTG AATGCGGCCA ACACCCAGCA GGATATCCAA 
GCGGCGGCGG CCTATTGGGC AGCAGCCTGC AAAGCAGTGG TGGCCAACAG CAGCGCCGAG 
GAGTTGCTGC AGTTGCAGCA GAACGACCAG ATCGAGATCA CCCGCCTGTC GACCACGGCG 
GAGCACTCCA TCGGATCCAG CGCCGGCGGC AACAATACAA AGAGCAAGCA GCAGAAGTGT 
CCCGTCTGTC CGTACATCTC GGAGAGCAAA TCCCAGATGA ACTACCACGT GTCGCTCCAC 
AAGCCCACGC AGTACGAGTG CCGGCTGTGC ACATTTGTGT GTGCCAAGAA ACAGCATCTG 
AGCAGCCACA TGAGAACGGT GCACCAGCAG CAGATGGTTG CATCGGGAGC AGGTGCAGCC 
GCAGGAGCAG CCGGAGGAGT AGGAGGAGGC GGAGCTTCCT CGCTGGGTCT GGACTTCAGT 
GTGGCCCTGC AGTTGGCCGC AGCTGCCAAG CAGGTGCAAC AACTGCCCGC CTCAACACCA 
CTCTCCATCG ATTTGTCCCA GATCCAGATG GATGCCGCCT CCGATGCGGA GCTCAATCCA 
CAGCAACTGC CGCCGGAGTA CCAATACAAG CTGATCAGCT ACTGTCCCAG GTGTCCGGCT 
CGCTTTGCCC AGAAGCACAA CGACGAGCGC AATGCCAAGC AAGAGCTGGA GCAGCATTTG 
CTGGCACACA GCGACCAGGA GCCGGAGGAT CAGGATCCGA GCTATGTCTG CGCCTACTGT 
GAGTATCGTA CATCGGAAGA GACCCTGCTG CAACTGCACC GTGCGGTGCA CATGTCCCAC 
TACCAGGAGA AGTGCCAGGA GCTGTACCGG AACTGCAAGG AGGATGTCGA GTATCCGGCA 
CCCAAGTTGC TGCAGCTGAC CGGTCCCGAG ACCATTTGGG TGGTAGACAA TGAGCTGAGC 
TTGCAGCTGC TGCAGCAAAC TACCGGAGGA GCAGTTAGTA CCTCCACCGG TGGGTTTGAC 
AACCAGAACT CTCTGCTCAA GAAGCAGCTG GAATCGGGCG GTGGCCAGAT GGTCGCACTT 
CCACGGGAAG CATCCGTGAC CAAAAATGAG GCGGAAGGCC CCGAGGAGGA CGAGGAGCGA 
CGTAGCAGCT CCACGCCGTC GACCAGCGCT TCGGTGGCCA CCAGTGATTT GGCCGCAGAT 
GGCAGCAGTG ATGCAGGATC CGTTGACATG CCCCAGTCCA TTCCGGCACC GGAACGCTGT 
CTCCATTGTC CATTCGAAAC GCAGCATCAC GAGGAACTGA TGCAGCATCT CCAGAAGCAC 
GCCTGCGTGA ATCCTCCACC TCCCAACTAC CAGAAATGTG CCCACTGTGA TTACAATGCC 
AATGAGGAGG CGGAAATGGA GGAGCACACG GCGCTGCATT TCAATGCCAG TGAGAAGCTC 
AAGTCCGTGG AGTTCTTCAC CTGCTACGAT CAGCTGGAGA TCAGCGTGGA GCGGGAACCG 
GAGGAAACCG CTGACTCAAA GGCGGAGCAG ACCGAGCACA ACAACAACCA GGACAATGTG 
ATGAACGCCA ATGTGCAGCA GCAGCAGCAG CCGAGCAGCA ACAGCCCAGA TGAACAGCCA 
GCTGACGAGG GGATGGAGGA ATCGTCGGCC AAGAAGTCCA GCACCAAGTT GATTCTGTAC 
AAGAACGATG GCGGTTTGAG TGTAAAGCTC TCCCAGGAAG AGGGCGCCAA TTCAGAGTTC 
CAGAAGAGCG AGAATATCAG CGATCGCCTG CGCAGAAGGA TCCTCCGGGG AAGTGCCAAC 
CAGACGGAGG ACGCCACTTC CACCTCCGGC CAGGAGCGAC CCGCAACACC GGACAAAATG 
ATACTGGTCA ATGCCCAGAC GGGCAAAGTC ATTTCCAGAA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016024830.1
CDS	1216..7200
Translation	MDLPSMVQRSGDTLIVRSVVSGNQLYTEQGGHQPASGNTGHMTSNSTSNTPAAQAGSSLLERHVERFRLQQLLQQQRQAAAAVAVVNSVQQQQQQQPQQVAIGMDAKEEGLPQCKIKRNYSCNHCAYFTQNPRYHLTHLRDVHGEKIVINKCKLCLYASRHFQKLVRHMKMVHGCTDGIPSGHGQARGKRGMSREARKRRLEESVGVMGGQSLTVTVPDVPTLEQVKRELLLQEEKLQRDIQAFNQRQREEQQREQQRELELVATSAYERQMQVLRDYERQSPAEPPTPSPTSGSATPPSNGEEPQNRLLKCSACEFTTLYRTQLRAHELAEHGKTKFFRCDKCSYVTHIKARFSKHVKYHSMPMIKCVTCDFRTPYKWNLDRHMKNHGGAGAFKCAACDFTADIKQSLTVHEMNHHVPPVGNAGSIWPRRQNKVGASEMCEDFLSDSAELEDQYNNNNVDDELDGVEDADDAMSGGEEQPMHLHHYGKRSKYDDEEEPTDLSQKGGCSSDTSSVGTPTPRAQRTMPNLIPISKGAKDVLNLSKETNASRSSLTEIASMFFNEKQISEMLDKSDVPQLSPATTVASQNSTRSQMTKKSSFMDKLKTGAQHENLVCQCGHVAKCLSESIIHGKSCHASAVIIDEDDAGLHEDDADDRLEIDEDDEDHHSHSALNLSVTGSTRCQHCRHRCKSSTDLMHHLKQCVEAIRCANEMYDSNSGESGDRRPDSQSLQQQAAVQQQRVCIWNKATKEIVAAAAASMQQEKNNHNLVKSPTGSQAASNEENSYYGVETAPGYGEVTKKMTPEEEAANSSLKKVYKCPHCSFWASTASRFHVHIVGHLNKKPFECSLCSYRSNWRWDITKHIRLKTIRDPSHKTAKVLMNDETGRRNYTKYNKYITLMKVTEEDGDPKLMKSGEMTPNQVASLAFLKDYAKVGSVSGQDITLEPVLPNKPNVLDDAHLADNLIRIPLLATMMNAAMAQQQHQQQQQKEHQSTMITPSVTISPVKRSNQASVMQGKPSDDLITEVHQEGNEKRTVYRCRKCNFGHQNRDAVLAHVKIHYQDASYPKSNSGNSSTSPLQVSVGGSPQFYMNKVFAAMCLSQTQSQSSPNSGSAGTSPAISAGLLQRAIQEAQHSPTPNASALSGLALALAGGKPQANTTKASAASILEHGEQKASQNEASADSLTSPNTTTTNSTTSRTTKDNTARSLQDLLTSPRSARNGNHTSNANSNSVVGNGLRLDAVYVASSTNNTTTPPPPPTNSVSKPNASPLPRHCRLKHSGDIRIETLERGASVSANAPPIYRPLGAGASNESQSHIHNPSNPITSNILKSGSNTNANSNINSNSAQPLLLTTKQLEQIFHTPLSASAAAVVNAANTQQDIQAAAAYWAAACKAVVANSSAEELLQLQQNDQIEITRLSTTAEHSIGSSAGGNNTKSKQQKCPVCPYISESKSQMNYHVSLHKPTQYECRLCTFVCAKKQHLSSHMRTVHQQQMVASGAGAAAGAAGGVGGGGASSLGLDFSVALQLAAAAKQVQQLPASTPLSIDLSQIQMDAASDAELNPQQLPPEYQYKLISYCPRCPARFAQKHNDERNAKQELEQHLLAHSDQEPEDQDPSYVCAYCEYRTSEETLLQLHRAVHMSHYQEKCQELYRNCKEDVEYPAPKLLQLTGPETIWVVDNELSLQLLQQTTGGAVSTSTGGFDNQNSLLKKQLESGGGQMVALPREASVTKNEAEGPEEDEERRSSSTPSTSASVATSDLAADGSSDAGSVDMPQSIPAPERCLHCPFETQHHEELMQHLQKHACVNPPPPNYQKCAHCDYNANEEAEMEEHTALHFNASEKLKSVEFFTCYDQLEISVEREPEETADSKAEQTEHNNNQDNVMNANVQQQQQPSSNSPDEQPADEGMEESSAKKSSTKLILYKNDGGLSVKLSQEEGANSEFQKSENISDRLRRRILRGSANQTEDATSTSGQERPATPDKMILVNAQTGKVISRK

Target ORF information:

RefSeq Version	XM_016178335.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant E (Dsim\GD22090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016178335.1

ORF Insert Sequence:

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGGTGAG CAGAAAGCGA GTCAAAACGA GGCAAGTGCC 
GACAGTCTGA CGTCGCCGAA CACCACCACC ACGAACTCAA CCACTAGTAG AACCACCAAA 
GACAACACCG CCAGATCGCT GCAGGATCTG CTCACCTCAC CGCGCAGTGC CAGAAATGGA 
AATCATACTT CTAACGCTAA CAGTAACTCG GTTGTGGGAA ATGGACTCCG GCTGGATGCC 
GTCTACGTCG CCTCATCCAC CAACAATACC ACAACACCAC CACCACCTCC CACCAACTCA 
GTCAGCAAGC CCAATGCATC GCCTTTGCCA CGGCACTGTC GCTTAAAGCA TTCCGGGGAT 
ATTCGGATCG AGACCCTTGA GCGAGGAGCC AGTGTCTCGG CGAACGCACC ACCCATCTAC 
CGACCCCTGG GAGCAGGTGC CAGCAACGAA TCCCAGTCCC ACATCCACAA TCCGTCGAAT 
CCCATCACCA GCAACATCCT AAAGTCGGGC AGCAACACGA ATGCCAACAG CAACATCAAC 
AGCAATAGTG CCCAGCCCCT GCTGCTGACC ACCAAGCAGC TGGAGCAGAT CTTCCACACA 
CCGCTCTCCG CCAGCGCCGC TGCAGTGGTG AATGCGGCCA ACACCCAGCA GGATATCCAA 
GCGGCGGCGG CCTATTGGGC AGCAGCCTGC AAAGCAGTGG TGGCCAACAG CAGCGCCGAG 
GAGTTGCTGC AGTTGCAGCA GAACGACCAG ATCGAGATCA CCCGCCTGTC GACCACGGCG 
GAGCACTCCA TCGGATCCAG CGCCGGCGGC AACAATACAA AGAGCAAGCA GCAGAAGTGT 
CCCGTCTGTC CGTACATCTC GGAGAGCAAA TCCCAGATGA ACTACCACGT GTCGCTCCAC 
AAGCCCACGC AGTACGAGTG CCGGCTGTGC ACATTTGTGT GTGCCAAGAA ACAGCATCTG 
AGCAGCCACA TGAGAACGGT GCACCAGCAG CAGATGGTTG CATCGGGAGC AGGTGCAGCC 
GCAGGAGCAG CCGGAGGAGT AGGAGGAGGC GGAGCTTCCT CGCTGGGTCT GGACTTCAGT 
GTGGCCCTGC AGTTGGCCGC AGCTGCCAAG CAGGTGCAAC AACTGCCCGC CTCAACACCA 
CTCTCCATCG ATTTGTCCCA GATCCAGATG GATGCCGCCT CCGATGCGGA GCTCAATCCA 
CAGCAACTGC CGCCGGAGTA CCAATACAAG CTGATCAGCT ACTGTCCCAG GTGTCCGGCT 
CGCTTTGCCC AGAAGCACAA CGACGAGCGC AATGCCAAGC AAGAGCTGGA GCAGCATTTG 
CTGGCACACA GCGACCAGGA GCCGGAGGAT CAGGATCCGA GCTATGTCTG CGCCTACTGT 
GAGTATCGTA CATCGGAAGA GACCCTGCTG CAACTGCACC GTGCGGTGCA CATGTCCCAC 
TACCAGGAGA AGTGCCAGGA GCTGTACCGG AACTGCAAGG AGGATGTCGA GTATCCGGCA 
CCCAAGTTGC TGCAGCTGAC CGGTCCCGAG ACCATTTGGG TGGTAGACAA TGAGCTGAGC 
TTGCAGCTGC TGCAGCAAAC TACCGGAGGA GCAGTTAGTA CCTCCACCGG TGGGTTTGAC 
AACCAGAACT CTCTGCTCAA GAAGCAGCTG GAATCGGGCG GTGGCCAGAT GGTCGCACTT 
CCACGGGAAG CATCCGTGAC CAAAAATGAG GCGGAAGGCC CCGAGGAGGA CGAGGAGCGA 
CGTAGCAGCT CCACGCCGTC GACCAGCGCT TCGGTGGCCA CCAGTGATTT GGCCGCAGAT 
GGCAGCAGTG ATGCAGGATC CGTTGACATG CCCCAGTCCA TTCCGGCACC GGAACGCTGT 
CTCCATTGTC CATTCGAAAC GCAGCATCAC GAGGAACTGA TGCAGCATCT CCAGAAGCAC 
GCCTGCGTGA ATCCTCCACC TCCCAACTAC CAGAAATGTG CCCACTGTGA TTACAATGCC 
AATGAGGAGG CGGAAATGGA GGAGCACACG GCGCTGCATT TCAATGCCAG TGAGAAGCTC 
AAGTCCGTGG AGTTCTTCAC CTGCTACGAT CAGCTGGAGA TCAGCGTGGA GCGGGAACCG 
GAGGAAACCG CTGACTCAAA GGCGGAGCAG ACCGAGCACA ACAACAACCA GGACAATGTG 
ATGAACGCCA ATGTGCAGCA GCAGCAGCAG CCGAGCAGCA ACAGCCCAGA TGAACAGCCA 
GCTGACGAGG GGATGGAGGA ATCGTCGGCC AAGAAGTCCA GCACCAAGTT GATTCTGTAC 
AAGAACGATG GCGGTTTGAG TGTAAAGCTC TCCCAGGAAG AGGGCGCCAA TTCAGAGTTC 
CAGAAGAGCG AGAATATCAG CGATCGCCTG CGCAGAAGGA TCCTCCGGGG AAGTGCCAAC 
CAGACGGAGG ACGCCACTTC CACCTCCGGC CAGGAGCGAC CCGCAACACC GGACAAAATG 
ATACTGGTCA ATGCCCAGAC GGGCAAAGTC ATTTCCAGAA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD22090 ( XM_016178336.1 ) cDNA ORF clone, Drosophila simulans -> XP_016024831.1 Drosophila simulans uncharacterized protein, transcript variant F (Dsim\GD22090), mRNA.

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CloneID	ODg18246
Clone ID Related Accession (Same CDS sequence)	XM_016178336.1
Accession Version	XM_016178336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGGTGAG CAGAAAGCGA GTCAAAACGA GCGGCACTGT 
CGCTTAAAGC ATTCCGGGGA TATTCGGATC GAGACCCTTG AGCGAGGAGC CAGTGTCTCG 
GCGAACGCAC CACCCATCTA CCGACCCCTG GGAGCAGGTG CCAGCAACGA ATCCCAGTCC 
CACATCCACA ATCCGTCGAA TCCCATCACC AGCAACATCC TAAAGTCGGG CAGCAACACG 
AATGCCAACA GCAACATCAA CAGCAATAGT GCCCAGCCCC TGCTGCTGAC CACCAAGCAG 
CTGGAGCAGA TCTTCCACAC ACCGCTCTCC GCCAGCGCCG CTGCAGTGGT GAATGCGGCC 
AACACCCAGC AGGATATCCA AGCGGCGGCG GCCTATTGGG CAGCAGCCTG CAAAGCAGTG 
GTGGCCAACA GCAGCGCCGA GGAGTTGCTG CAGTTGCAGC AGAACGACCA GATCGAGATC 
ACCCGCCTGT CGACCACGGC GGAGCACTCC ATCGGATCCA GCGCCGGCGG CAACAATACA 
AAGAGCAAGC AGCAGAAGTG TCCCGTCTGT CCGTACATCT CGGAGAGCAA ATCCCAGATG 
AACTACCACG TGTCGCTCCA CAAGCCCACG CAGTACGAGT GCCGGCTGTG CACATTTGTG 
TGTGCCAAGA AACAGCATCT GAGCAGCCAC ATGAGAACGG TGCACCAGCA GCAGATGGTT 
GCATCGGGAG CAGGTGCAGC CGCAGGAGCA GCCGGAGGAG TAGGAGGAGG CGGAGCTTCC 
TCGCTGGGTC TGGACTTCAG TGTGGCCCTG CAGTTGGCCG CAGCTGCCAA GCAGGTGCAA 
CAACTGCCCG CCTCAACACC ACTCTCCATC GATTTGTCCC AGATCCAGAT GGATGCCGCC 
TCCGATGCGG AGCTCAATCC ACAGCAACTG CCGCCGGAGT ACCAATACAA GCTGATCAGC 
TACTGTCCCA GGTGTCCGGC TCGCTTTGCC CAGAAGCACA ACGACGAGCG CAATGCCAAG 
CAAGAGCTGG AGCAGCATTT GCTGGCACAC AGCGACCAGG AGCCGGAGGA TCAGGATCCG 
AGCTATGTCT GCGCCTACTG TGAGTATCGT ACATCGGAAG AGACCCTGCT GCAACTGCAC 
CGTGCGGTGC ACATGTCCCA CTACCAGGAG AAGTGCCAGG AGCTGTACCG GAACTGCAAG 
GAGGATGTCG AGTATCCGGC ACCCAAGTTG CTGCAGCTGA CCGGTCCCGA GACCATTTGG 
GTGGTAGACA ATGAGCTGAG CTTGCAGCTG CTGCAGCAAA CTACCGGAGG AGCAGTTAGT 
ACCTCCACCG GTGGGTTTGA CAACCAGAAC TCTCTGCTCA AGAAGCAGCT GGAATCGGGC 
GGTGGCCAGA TGGTCGCACT TCCACGGGAA GCATCCGTGA CCAAAAATGA GGCGGAAGGC 
CCCGAGGAGG ACGAGGAGCG ACGTAGCAGC TCCACGCCGT CGACCAGCGC TTCGGTGGCC 
ACCAGTGATT TGGCCGCAGA TGGCAGCAGT GATGCAGGAT CCGTTGACAT GCCCCAGTCC 
ATTCCGGCAC CGGAACGCTG TCTCCATTGT CCATTCGAAA CGCAGCATCA CGAGGAACTG 
ATGCAGCATC TCCAGAAGCA CGCCTGCGTG AATCCTCCAC CTCCCAACTA CCAGAAATGT 
GCCCACTGTG ATTACAATGC CAATGAGGAG GCGGAAATGG AGGAGCACAC GGCGCTGCAT 
TTCAATGCCA GTGAGAAGCT CAAGTCCGTG GAGTTCTTCA CCTGCTACGA TCAGCTGGAG 
ATCAGCGTGG AGCGGGAACC GGAGGAAACC GCTGACTCAA AGGCGGAGCA GACCGAGCAC 
AACAACAACC AGGACAATGT GATGAACGCC AATGTGCAGC AGCAGCAGCA GCCGAGCAGC 
AACAGCCCAG ATGAACAGCC AGCTGACGAG GGGATGGAGG AATCGTCGGC CAAGAAGTCC 
AGCACCAAGT TGATTCTGTA CAAGAACGAT GGCGGTTTGA GTGTAAAGCT CTCCCAGGAA 
GAGGGCGCCA ATTCAGAGTT CCAGAAGAGC GAGAATATCA GCGATCGCCT GCGCAGAAGG 
ATCCTCCGGG GAAGTGCCAA CCAGACGGAG GACGCCACTT CCACCTCCGG CCAGGAGCGA 
CCCGCAACAC CGGACAAAAT GATACTGGTC AATGCCCAGA CGGGCAAAGT CATTTCCAGA 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016024831.1
CDS	1216..6921
Translation	MDLPSMVQRSGDTLIVRSVVSGNQLYTEQGGHQPASGNTGHMTSNSTSNTPAAQAGSSLLERHVERFRLQQLLQQQRQAAAAVAVVNSVQQQQQQQPQQVAIGMDAKEEGLPQCKIKRNYSCNHCAYFTQNPRYHLTHLRDVHGEKIVINKCKLCLYASRHFQKLVRHMKMVHGCTDGIPSGHGQARGKRGMSREARKRRLEESVGVMGGQSLTVTVPDVPTLEQVKRELLLQEEKLQRDIQAFNQRQREEQQREQQRELELVATSAYERQMQVLRDYERQSPAEPPTPSPTSGSATPPSNGEEPQNRLLKCSACEFTTLYRTQLRAHELAEHGKTKFFRCDKCSYVTHIKARFSKHVKYHSMPMIKCVTCDFRTPYKWNLDRHMKNHGGAGAFKCAACDFTADIKQSLTVHEMNHHVPPVGNAGSIWPRRQNKVGASEMCEDFLSDSAELEDQYNNNNVDDELDGVEDADDAMSGGEEQPMHLHHYGKRSKYDDEEEPTDLSQKGGCSSDTSSVGTPTPRAQRTMPNLIPISKGAKDVLNLSKETNASRSSLTEIASMFFNEKQISEMLDKSDVPQLSPATTVASQNSTRSQMTKKSSFMDKLKTGAQHENLVCQCGHVAKCLSESIIHGKSCHASAVIIDEDDAGLHEDDADDRLEIDEDDEDHHSHSALNLSVTGSTRCQHCRHRCKSSTDLMHHLKQCVEAIRCANEMYDSNSGESGDRRPDSQSLQQQAAVQQQRVCIWNKATKEIVAAAAASMQQEKNNHNLVKSPTGSQAASNEENSYYGVETAPGYGEVTKKMTPEEEAANSSLKKVYKCPHCSFWASTASRFHVHIVGHLNKKPFECSLCSYRSNWRWDITKHIRLKTIRDPSHKTAKVLMNDETGRRNYTKYNKYITLMKVTEEDGDPKLMKSGEMTPNQVASLAFLKDYAKVGSVSGQDITLEPVLPNKPNVLDDAHLADNLIRIPLLATMMNAAMAQQQHQQQQQKEHQSTMITPSVTISPVKRSNQASVMQGKPSDDLITEVHQEGNEKRTVYRCRKCNFGHQNRDAVLAHVKIHYQDASYPKSNSGNSSTSPLQVSVGGSPQFYMNKVFAAMCLSQTQSQSSPNSGSAGTSPAISAGLLQRAIQEAQHSPTPNASALSGLALALAGGKPQANTTKASAASILEHGEQKASQNERHCRLKHSGDIRIETLERGASVSANAPPIYRPLGAGASNESQSHIHNPSNPITSNILKSGSNTNANSNINSNSAQPLLLTTKQLEQIFHTPLSASAAAVVNAANTQQDIQAAAAYWAAACKAVVANSSAEELLQLQQNDQIEITRLSTTAEHSIGSSAGGNNTKSKQQKCPVCPYISESKSQMNYHVSLHKPTQYECRLCTFVCAKKQHLSSHMRTVHQQQMVASGAGAAAGAAGGVGGGGASSLGLDFSVALQLAAAAKQVQQLPASTPLSIDLSQIQMDAASDAELNPQQLPPEYQYKLISYCPRCPARFAQKHNDERNAKQELEQHLLAHSDQEPEDQDPSYVCAYCEYRTSEETLLQLHRAVHMSHYQEKCQELYRNCKEDVEYPAPKLLQLTGPETIWVVDNELSLQLLQQTTGGAVSTSTGGFDNQNSLLKKQLESGGGQMVALPREASVTKNEAEGPEEDEERRSSSTPSTSASVATSDLAADGSSDAGSVDMPQSIPAPERCLHCPFETQHHEELMQHLQKHACVNPPPPNYQKCAHCDYNANEEAEMEEHTALHFNASEKLKSVEFFTCYDQLEISVEREPEETADSKAEQTEHNNNQDNVMNANVQQQQQPSSNSPDEQPADEGMEESSAKKSSTKLILYKNDGGLSVKLSQEEGANSEFQKSENISDRLRRRILRGSANQTEDATSTSGQERPATPDKMILVNAQTGKVISRK

Target ORF information:

RefSeq Version	XM_016178336.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant F (Dsim\GD22090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016178336.1

ORF Insert Sequence:

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGGTGAG CAGAAAGCGA GTCAAAACGA GCGGCACTGT 
CGCTTAAAGC ATTCCGGGGA TATTCGGATC GAGACCCTTG AGCGAGGAGC CAGTGTCTCG 
GCGAACGCAC CACCCATCTA CCGACCCCTG GGAGCAGGTG CCAGCAACGA ATCCCAGTCC 
CACATCCACA ATCCGTCGAA TCCCATCACC AGCAACATCC TAAAGTCGGG CAGCAACACG 
AATGCCAACA GCAACATCAA CAGCAATAGT GCCCAGCCCC TGCTGCTGAC CACCAAGCAG 
CTGGAGCAGA TCTTCCACAC ACCGCTCTCC GCCAGCGCCG CTGCAGTGGT GAATGCGGCC 
AACACCCAGC AGGATATCCA AGCGGCGGCG GCCTATTGGG CAGCAGCCTG CAAAGCAGTG 
GTGGCCAACA GCAGCGCCGA GGAGTTGCTG CAGTTGCAGC AGAACGACCA GATCGAGATC 
ACCCGCCTGT CGACCACGGC GGAGCACTCC ATCGGATCCA GCGCCGGCGG CAACAATACA 
AAGAGCAAGC AGCAGAAGTG TCCCGTCTGT CCGTACATCT CGGAGAGCAA ATCCCAGATG 
AACTACCACG TGTCGCTCCA CAAGCCCACG CAGTACGAGT GCCGGCTGTG CACATTTGTG 
TGTGCCAAGA AACAGCATCT GAGCAGCCAC ATGAGAACGG TGCACCAGCA GCAGATGGTT 
GCATCGGGAG CAGGTGCAGC CGCAGGAGCA GCCGGAGGAG TAGGAGGAGG CGGAGCTTCC 
TCGCTGGGTC TGGACTTCAG TGTGGCCCTG CAGTTGGCCG CAGCTGCCAA GCAGGTGCAA 
CAACTGCCCG CCTCAACACC ACTCTCCATC GATTTGTCCC AGATCCAGAT GGATGCCGCC 
TCCGATGCGG AGCTCAATCC ACAGCAACTG CCGCCGGAGT ACCAATACAA GCTGATCAGC 
TACTGTCCCA GGTGTCCGGC TCGCTTTGCC CAGAAGCACA ACGACGAGCG CAATGCCAAG 
CAAGAGCTGG AGCAGCATTT GCTGGCACAC AGCGACCAGG AGCCGGAGGA TCAGGATCCG 
AGCTATGTCT GCGCCTACTG TGAGTATCGT ACATCGGAAG AGACCCTGCT GCAACTGCAC 
CGTGCGGTGC ACATGTCCCA CTACCAGGAG AAGTGCCAGG AGCTGTACCG GAACTGCAAG 
GAGGATGTCG AGTATCCGGC ACCCAAGTTG CTGCAGCTGA CCGGTCCCGA GACCATTTGG 
GTGGTAGACA ATGAGCTGAG CTTGCAGCTG CTGCAGCAAA CTACCGGAGG AGCAGTTAGT 
ACCTCCACCG GTGGGTTTGA CAACCAGAAC TCTCTGCTCA AGAAGCAGCT GGAATCGGGC 
GGTGGCCAGA TGGTCGCACT TCCACGGGAA GCATCCGTGA CCAAAAATGA GGCGGAAGGC 
CCCGAGGAGG ACGAGGAGCG ACGTAGCAGC TCCACGCCGT CGACCAGCGC TTCGGTGGCC 
ACCAGTGATT TGGCCGCAGA TGGCAGCAGT GATGCAGGAT CCGTTGACAT GCCCCAGTCC 
ATTCCGGCAC CGGAACGCTG TCTCCATTGT CCATTCGAAA CGCAGCATCA CGAGGAACTG 
ATGCAGCATC TCCAGAAGCA CGCCTGCGTG AATCCTCCAC CTCCCAACTA CCAGAAATGT 
GCCCACTGTG ATTACAATGC CAATGAGGAG GCGGAAATGG AGGAGCACAC GGCGCTGCAT 
TTCAATGCCA GTGAGAAGCT CAAGTCCGTG GAGTTCTTCA CCTGCTACGA TCAGCTGGAG 
ATCAGCGTGG AGCGGGAACC GGAGGAAACC GCTGACTCAA AGGCGGAGCA GACCGAGCAC 
AACAACAACC AGGACAATGT GATGAACGCC AATGTGCAGC AGCAGCAGCA GCCGAGCAGC 
AACAGCCCAG ATGAACAGCC AGCTGACGAG GGGATGGAGG AATCGTCGGC CAAGAAGTCC 
AGCACCAAGT TGATTCTGTA CAAGAACGAT GGCGGTTTGA GTGTAAAGCT CTCCCAGGAA 
GAGGGCGCCA ATTCAGAGTT CCAGAAGAGC GAGAATATCA GCGATCGCCT GCGCAGAAGG 
ATCCTCCGGG GAAGTGCCAA CCAGACGGAG GACGCCACTT CCACCTCCGG CCAGGAGCGA 
CCCGCAACAC CGGACAAAAT GATACTGGTC AATGCCCAGA CGGGCAAAGT CATTTCCAGA 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD22090 ( XM_016178332.1 ) cDNA ORF clone, Drosophila simulans -> XP_016024827.1 Drosophila simulans uncharacterized protein, transcript variant B (Dsim\GD22090), mRNA.

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CloneID	ODg18242
Clone ID Related Accession (Same CDS sequence)	XM_016178332.1
Accession Version	XM_016178332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGAT GACACCGGAG 
GAAGAGGCAG CCAACTCCTC GCTGAAGAAG GTGTACAAGT GTCCGCACTG CAGCTTCTGG 
GCCTCCACGG CGTCCCGCTT CCACGTCCAC ATCGTGGGCC ATCTGAACAA GAAGCCCTTT 
GAGTGCTCCC TCTGCTCGTA CCGCTCCAAC TGGCGCTGGG ACATCACCAA GCACATCCGA 
TTGAAGACGA TCCGCGATCC CTCGCACAAG ACGGCCAAGG TTCTGATGAA CGACGAGACG 
GGCCGTAGAA ACTACACCAA GTACAACAAA TACATTACCC TGATGAAGGT AACCGAGGAG 
GATGGTGATC CCAAGCTGAT GAAGTCCGGC GAGATGACAC CGAACCAGGT GGCCTCGTTG 
GCCTTCCTCA AGGACTATGC CAAGGTCGGC TCGGTTTCCG GGCAGGATAT CACATTGGAA 
CCAGTGCTTC CCAACAAACC CAATGTACTG GACGATGCCC ATTTGGCGGA CAACCTAATA 
CGGATTCCCT TGCTGGCCAC CATGATGAAT GCCGCTATGG CGCAACAGCA ACACCAGCAG 
CAGCAACAGA AGGAGCACCA GTCAACGATG ATCACGCCCT CGGTGACCAT TTCCCCGGTC 
AAGCGATCCA ATCAGGCATC CGTGATGCAG GGCAAGCCCA GCGACGACCT GATCACCGAG 
GTCCACCAGG AGGGCAATGA GAAGAGGACC GTCTATCGGT GCCGCAAGTG TAACTTCGGG 
CACCAGAATC GCGATGCTGT GCTGGCGCAC GTGAAGATTC ACTACCAGGA CGCCAGCTAC 
CCGAAGTCCA ACTCTGGGAA CTCCAGCACT TCGCCACTGC AGGTATCCGT GGGCGGTAGT 
CCACAATTCT ACATGAACAA GGTCTTTGCG GCCATGTGCC TGTCGCAGAC GCAGTCACAG 
TCGTCGCCCA ACTCCGGCTC CGCCGGAACG AGTCCGGCCA TCTCGGCCGG ACTGCTGCAG 
CGGGCCATCC AGGAGGCCCA GCACTCGCCC ACTCCGAACG CCAGCGCTCT GAGCGGACTT 
GCTTTGGCGT TGGCGGGCGG AAAGCCACAA GCCAATACGA CGAAAGCCAG TGCCGCCTCC 
ATTTTAGAGC ACGGTGAGCA GAAAGCGAGT CAAAACGAGG CAAGTGCCGA CAGTCTGACG 
TCGCCGAACA CCACCACCAC GAACTCAACC ACTAGTAGAA CCACCAAAGA CAACACCGCC 
AGATCGCTGC AGGATCTGCT CACCTCACCG CGCAGTGCCA GAAATGGAAA TCATACTTCT 
AACGCTAACA GTAACTCGGT TGTGGGAAAT GGACTCCGGC TGGATGCCGT CTACGTCGCC 
TCATCCACCA ACAATACCAC AACACCACCA CCACCTCCCA CCAACTCAGT CAGCAAGCCC 
AATGCATCGC CTTTGCCAGT AACTACCACT CCTACTCCTA TTTCTACTGC CACACCGCTG 
GCTCATCCAC TGGGTGCGGG TAACCACAGA TCATCCTCAT CCCATCACAA TGACCAAAAT 
CATGCTCCAC CCTCACACAT GGCCGGTGAT GGTTGCCACC TGGCGTCGGG TCTAACCCAT 
GTCCACACCA TAAGCACTAA CACGAAAGCC AATCCCAGCT CCAATTCCTT TCCCTCGTCA 
TCGTCGTCGT CGTCGTCATC ATCGTCTTCG GTGGCCTCCA CTTCATCGGC CGCCGGCTCA 
GCGTCCGCCT CGGCGTCCTC CACCACATCG TCGCCACCGC CGCCGGCAGC CTCATCCTCC 
GCCTCCGCCT ACATGACTGC CATGGCGCCG CAGAAACATG CGCCAACCGT TGCGCAGATG 
TCGCATCCGC AGACGAGATT CCATAGCCAT AGTCCAGGTA GTCCCAGCTT GCTGACCATG 
GCCGATGGCG ATCGACGCGA ACCGTCGCCG TATCGCTGCG GCCACTGCCA CCAGGTCTCG 
AATTGGAAGC ATGTCATCCA GCGGCACTGT CGCTTAAAGC ATTCCGGGGA TATTCGGATC 
GAGACCCTTG AGCGAGGAGC CAGTGTCTCG GCGAACGCAC CACCCATCTA CCGACCCCTG 
GGAGCAGGTG CCAGCAACGA ATCCCAGTCC CACATCCACA ATCCGTCGAA TCCCATCACC 
AGCAACATCC TAAAGTCGGG CAGCAACACG AATGCCAACA GCAACATCAA CAGCAATAGT 
GCCCAGCCCC TGCTGCTGAC CACCAAGCAG CTGGAGCAGA TCTTCCACAC ACCGCTCTCC 
GCCAGCGCCG CTGCAGTGGT GAATGCGGCC AACACCCAGC AGGATATCCA AGCGGCGGCG 
GCCTATTGGG CAGCAGCCTG CAAAGCAGTG GTGGCCAACA GCAGCGCCGA GGAGTTGCTG 
CAGTTGCAGC AGAACGACCA GATCGAGATC ACCCGCCTGT CGACCACGGC GGAGCACTCC 
ATCGGATCCA GCGCCGGCGG CAACAATACA AAGAGCAAGC AGCAGAAGTG TCCCGTCTGT 
CCGTACATCT CGGAGAGCAA ATCCCAGATG AACTACCACG TGTCGCTCCA CAAGCCCACG 
CAGTACGAGT GCCGGCTGTG CACATTTGTG TGTGCCAAGA AACAGCATCT GAGCAGCCAC 
ATGAGAACGG TGCACCAGCA GCAGATGGTT GCATCGGGAG CAGGTGCAGC CGCAGGAGCA 
GCCGGAGGAG TAGGAGGAGG CGGAGCTTCC TCGCTGGGTC TGGACTTCAG TGTGGCCCTG 
CAGTTGGCCG CAGCTGCCAA GCAGGTGCAA CAACTGCCCG CCTCAACACC ACTCTCCATC 
GATTTGTCCC AGATCCAGAT GGATGCCGCC TCCGATGCGG AGCTCAATCC ACAGCAACTG 
CCGCCGGAGT ACCAATACAA GCTGATCAGC TACTGTCCCA GGTGTCCGGC TCGCTTTGCC 
CAGAAGCACA ACGACGAGCG CAATGCCAAG CAAGAGCTGG AGCAGCATTT GCTGGCACAC 
AGCGACCAGG AGCCGGAGGA TCAGGATCCG AGCTATGTCT GCGCCTACTG TGAGTATCGT 
ACATCGGAAG AGACCCTGCT GCAACTGCAC CGTGCGGTGC ACATGTCCCA CTACCAGGAG 
AAGTGCCAGG AGCTGTACCG GAACTGCAAG GAGGATGTCG AGTATCCGGC ACCCAAGTTG 
CTGCAGCTGA CCGGTCCCGA GACCATTTGG GTGGTAGACA ATGAGCTGAG CTTGCAGCTG 
CTGCAGCAAA CTACCGGAGG AGCAGTTAGT ACCTCCACCG GTGGGTTTGA CAACCAGAAC 
TCTCTGCTCA AGAAGCAGCT GGAATCGGGC GGTGGCCAGA TGGTCGCACT TCCACGGGAA 
GCATCCGTGA CCAAAAATGA GGCGGAAGGC CCCGAGGAGG ACGAGGAGCG ACGTAGCAGC 
TCCACGCCGT CGACCAGCGC TTCGGTGGCC ACCAGTGATT TGGCCGCAGA TGGCAGCAGT 
GATGCAGGAT CCGTTGACAT GCCCCAGTCC ATTCCGGCAC CGGAACGCTG TCTCCATTGT 
CCATTCGAAA CGCAGCATCA CGAGGAACTG ATGCAGCATC TCCAGAAGCA CGCCTGCGTG 
AATCCTCCAC CTCCCAACTA CCAGAAATGT GCCCACTGTG ATTACAATGC CAATGAGGAG 
GCGGAAATGG AGGAGCACAC GGCGCTGCAT TTCAATGCCA GTGAGAAGCT CAAGTCCGTG 
GAGTTCTTCA CCTGCTACGA TCAGCTGGAG ATCAGCGTGG AGCGGGAACC GGAGGAAACC 
GCTGACTCAA AGGCGGAGCA GACCGAGCAC AACAACAACC AGGACAATGT GATGAACGCC 
AATGTGCAGC AGCAGCAGCA GCCGAGCAGC AACAGCCCAG ATGAACAGCC AGCTGACGAG 
GGGATGGAGG AATCGTCGGC CAAGAAGTCC AGCACCAAGT TGATTCTGTA CAAGAACGAT 
GGCGGTTTGA GTGTAAAGCT CTCCCAGGAA GAGGGCGCCA ATTCAGAGTT CCAGAAGAGC 
GAGAATATCA GCGATCGCCT GCGCAGAAGG ATCCTCCGGG GAAGTGCCAA CCAGACGGAG 
GACGCCACTT CCACCTCCGG CCAGGAGCGA CCCGCAACAC CGGACAAAAT GATACTGGTC 
AATGCCCAGA CGGGCAAAGT CATTTCCAGA AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016024827.1
CDS	1217..7732
Translation	MDLPSMVQRSGDTLIVRSVVSGNQLYTEQGGHQPASGNTGHMTSNSTSNTPAAQAGSSLLERHVERFRLQQLLQQQRQAAAAVAVVNSVQQQQQQQPQQVAIGMDAKEEGLPQCKIKRNYSCNHCAYFTQNPRYHLTHLRDVHGEKIVINKCKLCLYASRHFQKLVRHMKMVHGCTDGIPSGHGQARGKRGMSREARKRRLEESVGVMGGQSLTVTVPDVPTLEQVKRELLLQEEKLQRDIQAFNQRQREEQQREQQRELELVATSAYERQMQVLRDYERQSPAEPPTPSPTSGSATPPSNGEEPQNRLLKCSACEFTTLYRTQLRAHELAEHGKTKFFRCDKCSYVTHIKARFSKHVKYHSMPMIKCVTCDFRTPYKWNLDRHMKNHGGAGAFKCAACDFTADIKQSLTVHEMNHHVPPVGNAGSIWPRRQNKVGASEMCEDFLSDSAELEDQYNNNNVDDELDGVEDADDAMSGGEEQPMHLHHYGKRSKYDDEEEPTDLSQKGGCSSDTSSVGTPTPRAQRTMPNLIPISKGAKDVLNLSKETNASRSSLTEIASMFFNEKQISEMLDKSDVPQLSPATTVASQNSTRSQMTKKSSFMDKLKTGAQHENLVCQCGHVAKCLSESIIHGKSCHASAVIIDEDDAGLHEDDADDRLEIDEDDEDHHSHSALNLSVTGSTRCQHCRHRCKSSTDLMHHLKQCVEAIRCANEMYDSNSGESGDRRPDSQSLQQQAAVQQQRVCIWNKATKEIVAAAAASMQQEKNNHNLVKSPTGSQAASNEENSYYGVETAPGYGEMTPEEEAANSSLKKVYKCPHCSFWASTASRFHVHIVGHLNKKPFECSLCSYRSNWRWDITKHIRLKTIRDPSHKTAKVLMNDETGRRNYTKYNKYITLMKVTEEDGDPKLMKSGEMTPNQVASLAFLKDYAKVGSVSGQDITLEPVLPNKPNVLDDAHLADNLIRIPLLATMMNAAMAQQQHQQQQQKEHQSTMITPSVTISPVKRSNQASVMQGKPSDDLITEVHQEGNEKRTVYRCRKCNFGHQNRDAVLAHVKIHYQDASYPKSNSGNSSTSPLQVSVGGSPQFYMNKVFAAMCLSQTQSQSSPNSGSAGTSPAISAGLLQRAIQEAQHSPTPNASALSGLALALAGGKPQANTTKASAASILEHGEQKASQNEASADSLTSPNTTTTNSTTSRTTKDNTARSLQDLLTSPRSARNGNHTSNANSNSVVGNGLRLDAVYVASSTNNTTTPPPPPTNSVSKPNASPLPVTTTPTPISTATPLAHPLGAGNHRSSSSHHNDQNHAPPSHMAGDGCHLASGLTHVHTISTNTKANPSSNSFPSSSSSSSSSSSSVASTSSAAGSASASASSTTSSPPPPAASSSASAYMTAMAPQKHAPTVAQMSHPQTRFHSHSPGSPSLLTMADGDRREPSPYRCGHCHQVSNWKHVIQRHCRLKHSGDIRIETLERGASVSANAPPIYRPLGAGASNESQSHIHNPSNPITSNILKSGSNTNANSNINSNSAQPLLLTTKQLEQIFHTPLSASAAAVVNAANTQQDIQAAAAYWAAACKAVVANSSAEELLQLQQNDQIEITRLSTTAEHSIGSSAGGNNTKSKQQKCPVCPYISESKSQMNYHVSLHKPTQYECRLCTFVCAKKQHLSSHMRTVHQQQMVASGAGAAAGAAGGVGGGGASSLGLDFSVALQLAAAAKQVQQLPASTPLSIDLSQIQMDAASDAELNPQQLPPEYQYKLISYCPRCPARFAQKHNDERNAKQELEQHLLAHSDQEPEDQDPSYVCAYCEYRTSEETLLQLHRAVHMSHYQEKCQELYRNCKEDVEYPAPKLLQLTGPETIWVVDNELSLQLLQQTTGGAVSTSTGGFDNQNSLLKKQLESGGGQMVALPREASVTKNEAEGPEEDEERRSSSTPSTSASVATSDLAADGSSDAGSVDMPQSIPAPERCLHCPFETQHHEELMQHLQKHACVNPPPPNYQKCAHCDYNANEEAEMEEHTALHFNASEKLKSVEFFTCYDQLEISVEREPEETADSKAEQTEHNNNQDNVMNANVQQQQQPSSNSPDEQPADEGMEESSAKKSSTKLILYKNDGGLSVKLSQEEGANSEFQKSENISDRLRRRILRGSANQTEDATSTSGQERPATPDKMILVNAQTGKVISRK

Target ORF information:

RefSeq Version	XM_016178332.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant B (Dsim\GD22090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016178332.1

ORF Insert Sequence:

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGAT GACACCGGAG 
GAAGAGGCAG CCAACTCCTC GCTGAAGAAG GTGTACAAGT GTCCGCACTG CAGCTTCTGG 
GCCTCCACGG CGTCCCGCTT CCACGTCCAC ATCGTGGGCC ATCTGAACAA GAAGCCCTTT 
GAGTGCTCCC TCTGCTCGTA CCGCTCCAAC TGGCGCTGGG ACATCACCAA GCACATCCGA 
TTGAAGACGA TCCGCGATCC CTCGCACAAG ACGGCCAAGG TTCTGATGAA CGACGAGACG 
GGCCGTAGAA ACTACACCAA GTACAACAAA TACATTACCC TGATGAAGGT AACCGAGGAG 
GATGGTGATC CCAAGCTGAT GAAGTCCGGC GAGATGACAC CGAACCAGGT GGCCTCGTTG 
GCCTTCCTCA AGGACTATGC CAAGGTCGGC TCGGTTTCCG GGCAGGATAT CACATTGGAA 
CCAGTGCTTC CCAACAAACC CAATGTACTG GACGATGCCC ATTTGGCGGA CAACCTAATA 
CGGATTCCCT TGCTGGCCAC CATGATGAAT GCCGCTATGG CGCAACAGCA ACACCAGCAG 
CAGCAACAGA AGGAGCACCA GTCAACGATG ATCACGCCCT CGGTGACCAT TTCCCCGGTC 
AAGCGATCCA ATCAGGCATC CGTGATGCAG GGCAAGCCCA GCGACGACCT GATCACCGAG 
GTCCACCAGG AGGGCAATGA GAAGAGGACC GTCTATCGGT GCCGCAAGTG TAACTTCGGG 
CACCAGAATC GCGATGCTGT GCTGGCGCAC GTGAAGATTC ACTACCAGGA CGCCAGCTAC 
CCGAAGTCCA ACTCTGGGAA CTCCAGCACT TCGCCACTGC AGGTATCCGT GGGCGGTAGT 
CCACAATTCT ACATGAACAA GGTCTTTGCG GCCATGTGCC TGTCGCAGAC GCAGTCACAG 
TCGTCGCCCA ACTCCGGCTC CGCCGGAACG AGTCCGGCCA TCTCGGCCGG ACTGCTGCAG 
CGGGCCATCC AGGAGGCCCA GCACTCGCCC ACTCCGAACG CCAGCGCTCT GAGCGGACTT 
GCTTTGGCGT TGGCGGGCGG AAAGCCACAA GCCAATACGA CGAAAGCCAG TGCCGCCTCC 
ATTTTAGAGC ACGGTGAGCA GAAAGCGAGT CAAAACGAGG CAAGTGCCGA CAGTCTGACG 
TCGCCGAACA CCACCACCAC GAACTCAACC ACTAGTAGAA CCACCAAAGA CAACACCGCC 
AGATCGCTGC AGGATCTGCT CACCTCACCG CGCAGTGCCA GAAATGGAAA TCATACTTCT 
AACGCTAACA GTAACTCGGT TGTGGGAAAT GGACTCCGGC TGGATGCCGT CTACGTCGCC 
TCATCCACCA ACAATACCAC AACACCACCA CCACCTCCCA CCAACTCAGT CAGCAAGCCC 
AATGCATCGC CTTTGCCAGT AACTACCACT CCTACTCCTA TTTCTACTGC CACACCGCTG 
GCTCATCCAC TGGGTGCGGG TAACCACAGA TCATCCTCAT CCCATCACAA TGACCAAAAT 
CATGCTCCAC CCTCACACAT GGCCGGTGAT GGTTGCCACC TGGCGTCGGG TCTAACCCAT 
GTCCACACCA TAAGCACTAA CACGAAAGCC AATCCCAGCT CCAATTCCTT TCCCTCGTCA 
TCGTCGTCGT CGTCGTCATC ATCGTCTTCG GTGGCCTCCA CTTCATCGGC CGCCGGCTCA 
GCGTCCGCCT CGGCGTCCTC CACCACATCG TCGCCACCGC CGCCGGCAGC CTCATCCTCC 
GCCTCCGCCT ACATGACTGC CATGGCGCCG CAGAAACATG CGCCAACCGT TGCGCAGATG 
TCGCATCCGC AGACGAGATT CCATAGCCAT AGTCCAGGTA GTCCCAGCTT GCTGACCATG 
GCCGATGGCG ATCGACGCGA ACCGTCGCCG TATCGCTGCG GCCACTGCCA CCAGGTCTCG 
AATTGGAAGC ATGTCATCCA GCGGCACTGT CGCTTAAAGC ATTCCGGGGA TATTCGGATC 
GAGACCCTTG AGCGAGGAGC CAGTGTCTCG GCGAACGCAC CACCCATCTA CCGACCCCTG 
GGAGCAGGTG CCAGCAACGA ATCCCAGTCC CACATCCACA ATCCGTCGAA TCCCATCACC 
AGCAACATCC TAAAGTCGGG CAGCAACACG AATGCCAACA GCAACATCAA CAGCAATAGT 
GCCCAGCCCC TGCTGCTGAC CACCAAGCAG CTGGAGCAGA TCTTCCACAC ACCGCTCTCC 
GCCAGCGCCG CTGCAGTGGT GAATGCGGCC AACACCCAGC AGGATATCCA AGCGGCGGCG 
GCCTATTGGG CAGCAGCCTG CAAAGCAGTG GTGGCCAACA GCAGCGCCGA GGAGTTGCTG 
CAGTTGCAGC AGAACGACCA GATCGAGATC ACCCGCCTGT CGACCACGGC GGAGCACTCC 
ATCGGATCCA GCGCCGGCGG CAACAATACA AAGAGCAAGC AGCAGAAGTG TCCCGTCTGT 
CCGTACATCT CGGAGAGCAA ATCCCAGATG AACTACCACG TGTCGCTCCA CAAGCCCACG 
CAGTACGAGT GCCGGCTGTG CACATTTGTG TGTGCCAAGA AACAGCATCT GAGCAGCCAC 
ATGAGAACGG TGCACCAGCA GCAGATGGTT GCATCGGGAG CAGGTGCAGC CGCAGGAGCA 
GCCGGAGGAG TAGGAGGAGG CGGAGCTTCC TCGCTGGGTC TGGACTTCAG TGTGGCCCTG 
CAGTTGGCCG CAGCTGCCAA GCAGGTGCAA CAACTGCCCG CCTCAACACC ACTCTCCATC 
GATTTGTCCC AGATCCAGAT GGATGCCGCC TCCGATGCGG AGCTCAATCC ACAGCAACTG 
CCGCCGGAGT ACCAATACAA GCTGATCAGC TACTGTCCCA GGTGTCCGGC TCGCTTTGCC 
CAGAAGCACA ACGACGAGCG CAATGCCAAG CAAGAGCTGG AGCAGCATTT GCTGGCACAC 
AGCGACCAGG AGCCGGAGGA TCAGGATCCG AGCTATGTCT GCGCCTACTG TGAGTATCGT 
ACATCGGAAG AGACCCTGCT GCAACTGCAC CGTGCGGTGC ACATGTCCCA CTACCAGGAG 
AAGTGCCAGG AGCTGTACCG GAACTGCAAG GAGGATGTCG AGTATCCGGC ACCCAAGTTG 
CTGCAGCTGA CCGGTCCCGA GACCATTTGG GTGGTAGACA ATGAGCTGAG CTTGCAGCTG 
CTGCAGCAAA CTACCGGAGG AGCAGTTAGT ACCTCCACCG GTGGGTTTGA CAACCAGAAC 
TCTCTGCTCA AGAAGCAGCT GGAATCGGGC GGTGGCCAGA TGGTCGCACT TCCACGGGAA 
GCATCCGTGA CCAAAAATGA GGCGGAAGGC CCCGAGGAGG ACGAGGAGCG ACGTAGCAGC 
TCCACGCCGT CGACCAGCGC TTCGGTGGCC ACCAGTGATT TGGCCGCAGA TGGCAGCAGT 
GATGCAGGAT CCGTTGACAT GCCCCAGTCC ATTCCGGCAC CGGAACGCTG TCTCCATTGT 
CCATTCGAAA CGCAGCATCA CGAGGAACTG ATGCAGCATC TCCAGAAGCA CGCCTGCGTG 
AATCCTCCAC CTCCCAACTA CCAGAAATGT GCCCACTGTG ATTACAATGC CAATGAGGAG 
GCGGAAATGG AGGAGCACAC GGCGCTGCAT TTCAATGCCA GTGAGAAGCT CAAGTCCGTG 
GAGTTCTTCA CCTGCTACGA TCAGCTGGAG ATCAGCGTGG AGCGGGAACC GGAGGAAACC 
GCTGACTCAA AGGCGGAGCA GACCGAGCAC AACAACAACC AGGACAATGT GATGAACGCC 
AATGTGCAGC AGCAGCAGCA GCCGAGCAGC AACAGCCCAG ATGAACAGCC AGCTGACGAG 
GGGATGGAGG AATCGTCGGC CAAGAAGTCC AGCACCAAGT TGATTCTGTA CAAGAACGAT 
GGCGGTTTGA GTGTAAAGCT CTCCCAGGAA GAGGGCGCCA ATTCAGAGTT CCAGAAGAGC 
GAGAATATCA GCGATCGCCT GCGCAGAAGG ATCCTCCGGG GAAGTGCCAA CCAGACGGAG 
GACGCCACTT CCACCTCCGG CCAGGAGCGA CCCGCAACAC CGGACAAAAT GATACTGGTC 
AATGCCCAGA CGGGCAAAGT CATTTCCAGA AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD22090 ( XM_016178337.1 ) cDNA ORF clone, Drosophila simulans -> XP_016024832.1 Drosophila simulans uncharacterized protein, transcript variant G (Dsim\GD22090), mRNA.

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CloneID	ODg18247
Clone ID Related Accession (Same CDS sequence)	XM_016178337.1
Accession Version	XM_016178337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGGTGAG CAGAAAGCGA GTCAAAACGA GGCAAGTGCC 
GACAGTCTGA CGTCGCCGAA CACCACCACC ACGAACTCAA CCACTAGTAG AACCACCAAA 
GACAACACCG CCAGATCGCT GCAGGATCTG CTCACCTCAC CGCGCAGTGC CAGAAATGGA 
AATCATACTT CTAACGCTAA CAGTAACTCG GTTGTGGGAA ATGGACTCCG GCTGGATGCC 
GTCTACGTCG CCTCATCCAC CAACAATACC ACAACACCAC CACCACCTCC CACCAACTCA 
GTCAGCAAGC CCAATGCATC GCCTTTGCCA GTAACTACCA CTCCTACTCC TATTTCTACT 
GCCACACCGC TGGCTCATCC ACTGGGTGCG GGTAACCACA GATCATCCTC ATCCCATCAC 
AATGACCAAA ATCATGCTCC ACCCTCACAC ATGGCCGGTG ATGGTTGCCA CCTGGCGTCG 
GGTCTAACCC ATGTCCACAC CATAAGCACT AACACGAAAG CCAATCCCAG CTCCAATTCC 
TTTCCCTCGT CATCGTCGTC GTCGTCGTCA TCATCGTCTT CGGTGGCCTC CACTTCATCG 
GCCGCCGGCT CAGCGTCCGC CTCGGCGTCC TCCACCACAT CGTCGCCACC GCCGCCGGCA 
GCCTCATCCT CCGCCTCCGC CTACATGACT GCCATGGCGC CGCAGAAACA TGCGCCAACC 
GTTGCGCAGA TGTCGCATCC GCAGACGAGA TTCCATAGCC ATAGTCCAGG TAGTCCCAGC 
TTGCTGACCA TGGCCGATGG CGATCGACGC GAACCGTCGC CGTATCGCTG CGGCCACTGC 
CACCAGGTCT CGAATTGGAA GCATGTCATC CAGCGGCACT GTCGCTTAAA GCATTCCGGG 
GATATTCGGA TCGAGACCCT TGAGCGAGGA GCCAGTGTCT CGGCGAACGC ACCACCCATC 
TACCGACCCC TGGGAGCAGG TGCCAGCAAC GAATCCCAGT CCCACATCCA CAATCCGTCG 
AATCCCATCA CCAGCAACAT CCTAAAGTCG GGCAGCAACA CGAATGCCAA CAGCAACATC 
AACAGCAATA GTGCCCAGCC CCTGCTGCTG ACCACCAAGC AGCTGGAGCA GATCTTCCAC 
ACACCGCTCT CCGCCAGCGC CGCTGCAGTG GTGAATGCGG CCAACACCCA GCAGGATATC 
CAAGCGGCGG CGGCCTATTG GGCAGCAGCC TGCAAAGCAG TGGTGGCCAA CAGCAGCGCC 
GAGGAGTTGC TGCAGTTGCA GCAGAACGAC CAGATCGAGA TCACCCGCCT GTCGACCACG 
GCGGAGCACT CCATCGGATC CAGCGCCGGC GGCAACAATA CAAAGAGCAA GCAGCAGAAG 
TGTCCCGTCT GTCCGTACAT CTCGGAGAGC AAATCCCAGA TGAACTACCA CGTGTCGCTC 
CACAAGCCCA CGCAGTACGA GTGCCGGCTG TGCACATTTG TGTGTGCCAA GAAACAGCAT 
CTGAGCAGCC ACATGAGAAC GGTGCACCAG CAGCAGATGG TTGCATCGGG AGCAGGTGCA 
GCCGCAGGAG CAGCCGGAGG AGTAGGAGGA GGCGGAGCTT CCTCGCTGGG TCTGGACTTC 
AGTGTGGCCC TGCAGTTGGC CGCAGCTGCC AAGCAGGTGC AACAACTGCC CGCCTCAACA 
CCACTCTCCA TCGATTTGTC CCAGATCCAG ATGGATGCCG CCTCCGATGC GGAGCTCAAT 
CCACAGCAAC TGCCGCCGGA GTACCAATAC AAGCTGATCA GCTACTGTCC CAGGTGTCCG 
GCTCGCTTTG CCCAGAAGCA CAACGACGAG CGCAATGCCA AGCAAGAGCT GGAGCAGCAT 
TTGCTGGCAC ACAGCGACCA GGAGCCGGAG GATCAGGATC CGAGCTATGT CTGCGCCTAC 
TGTGAGTATC GTACATCGGA AGAGACCCTG CTGCAACTGC ACCGTGCGGT GCACATGTCC 
CACTACCAGG AGAAGTGCCA GGAGCTGTAC CGGAACTGCA AGGAGGATGT CGAGTATCCG 
GCACCCAAGT TGCTGCAGCT GACCGGTCCC GAGACCATTT GGGTGGTAGA CAATGAGCTG 
AGCTTGCAGC TGCTGCAGCA AACTACCGGA GGAGCAGTTA GTACCTCCAC CGGTGGGTTT 
GACAACCAGA ACTCTCTGCT CAAGAAGCAG CTGGAATCGG GCGGTGGCCA GATGGTCGCA 
CTTCCACGGG AAGCATCCGT GACCAAAAAT GAGGCGGAAG GCCCCGAGGA GGACGAGGAG 
CGACGTAGCA GCTCCACGCC GTCGACCAGC GCTTCGGTGG CCACCAGTGA TTTGGCCGCA 
GATGGCAGCA GTGATGCAGG ATCCGTTGAC ATGCCCCAGT CCATTCCGGC ACCGGAACGC 
TGTCTCCATT GTCCATTCGA AACGCAGCAT CACGAGGAAC TGATGCAGCA TCTCCAGAAG 
CACGCCTGCG TGAATCCTCC ACCTCCCAAC TACCAGAAAT GTGCCCACTG TGATTACAAT 
GCCAATGAGG AGGCGGAAAT GGAGGAGCAC ACGGCGCTGC ATTTCAATGC CAGTGAGAAG 
CTCAAGTCCG TGGAGTTCTT CACCTGCTAC GATCAGCTGG AGATCAGCGT GGAGCGGGAA 
CCGGAGGAAA CCGCTGACTC AAAGGCGGAG CAGACCGAGC ACAACAACAA CCAGGACAAT 
GTGATGAACG CCAATGTGCA GCAGCAGCAG CAGCCGAGCA GCAACAGCCC AGATGAACAG 
CCAGCTGACG AGGGGATGGA GGAATCGTCG GCCAAGAAGT CCAGCACCAA GTTGATTCTG 
TACAAGAACG ATGGCGGTTT GAGTGTAAAG CTCTCCCAGG AAGAGGGCGC CAATTCAGAG 
TTCCAGAAGA GCGAGAATAT CAGCGATCGC CTGCGCAGAA GGATCCTCCG GGGAAGTGCC 
AACCAGACGG AGGACGCCAC TTCCACCTCC GGCCAGGAGC GACCCGCAAC ACCGGACAAA 
ATGATACTGG TCAATGCCCA GACGGGCAAA GTCATTTCCA GAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016024832.1
CDS	1217..7744
Translation	MDLPSMVQRSGDTLIVRSVVSGNQLYTEQGGHQPASGNTGHMTSNSTSNTPAAQAGSSLLERHVERFRLQQLLQQQRQAAAAVAVVNSVQQQQQQQPQQVAIGMDAKEEGLPQCKIKRNYSCNHCAYFTQNPRYHLTHLRDVHGEKIVINKCKLCLYASRHFQKLVRHMKMVHGCTDGIPSGHGQARGKRGMSREARKRRLEESVGVMGGQSLTVTVPDVPTLEQVKRELLLQEEKLQRDIQAFNQRQREEQQREQQRELELVATSAYERQMQVLRDYERQSPAEPPTPSPTSGSATPPSNGEEPQNRLLKCSACEFTTLYRTQLRAHELAEHGKTKFFRCDKCSYVTHIKARFSKHVKYHSMPMIKCVTCDFRTPYKWNLDRHMKNHGGAGAFKCAACDFTADIKQSLTVHEMNHHVPPVGNAGSIWPRRQNKVGASEMCEDFLSDSAELEDQYNNNNVDDELDGVEDADDAMSGGEEQPMHLHHYGKRSKYDDEEEPTDLSQKGGCSSDTSSVGTPTPRAQRTMPNLIPISKGAKDVLNLSKETNASRSSLTEIASMFFNEKQISEMLDKSDVPQLSPATTVASQNSTRSQMTKKSSFMDKLKTGAQHENLVCQCGHVAKCLSESIIHGKSCHASAVIIDEDDAGLHEDDADDRLEIDEDDEDHHSHSALNLSVTGSTRCQHCRHRCKSSTDLMHHLKQCVEAIRCANEMYDSNSGESGDRRPDSQSLQQQAAVQQQRVCIWNKATKEIVAAAAASMQQEKNNHNLVKSPTGSQAASNEENSYYGVETAPGYGEVTKKMTPEEEAANSSLKKVYKCPHCSFWASTASRFHVHIVGHLNKKPFECSLCSYRSNWRWDITKHIRLKTIRDPSHKTAKVLMNDETGRRNYTKYNKYITLMKVTEEDGDPKLMKSGEMTPNQVASLAFLKDYAKVGSVSGQDITLEPVLPNKPNVLDDAHLADNLIRIPLLATMMNAAMAQQQHQQQQQKEHQSTMITPSVTISPVKRSNQASVMQGKPSDDLITEVHQEGNEKRTVYRCRKCNFGHQNRDAVLAHVKIHYQDASYPKSNSGNSSTSPLQVSVGGSPQFYMNKVFAAMCLSQTQSQSSPNSGSAGTSPAISAGLLQRAIQEAQHSPTPNASALSGLALALAGGKPQANTTKASAASILEHGEQKASQNEASADSLTSPNTTTTNSTTSRTTKDNTARSLQDLLTSPRSARNGNHTSNANSNSVVGNGLRLDAVYVASSTNNTTTPPPPPTNSVSKPNASPLPVTTTPTPISTATPLAHPLGAGNHRSSSSHHNDQNHAPPSHMAGDGCHLASGLTHVHTISTNTKANPSSNSFPSSSSSSSSSSSSVASTSSAAGSASASASSTTSSPPPPAASSSASAYMTAMAPQKHAPTVAQMSHPQTRFHSHSPGSPSLLTMADGDRREPSPYRCGHCHQVSNWKHVIQRHCRLKHSGDIRIETLERGASVSANAPPIYRPLGAGASNESQSHIHNPSNPITSNILKSGSNTNANSNINSNSAQPLLLTTKQLEQIFHTPLSASAAAVVNAANTQQDIQAAAAYWAAACKAVVANSSAEELLQLQQNDQIEITRLSTTAEHSIGSSAGGNNTKSKQQKCPVCPYISESKSQMNYHVSLHKPTQYECRLCTFVCAKKQHLSSHMRTVHQQQMVASGAGAAAGAAGGVGGGGASSLGLDFSVALQLAAAAKQVQQLPASTPLSIDLSQIQMDAASDAELNPQQLPPEYQYKLISYCPRCPARFAQKHNDERNAKQELEQHLLAHSDQEPEDQDPSYVCAYCEYRTSEETLLQLHRAVHMSHYQEKCQELYRNCKEDVEYPAPKLLQLTGPETIWVVDNELSLQLLQQTTGGAVSTSTGGFDNQNSLLKKQLESGGGQMVALPREASVTKNEAEGPEEDEERRSSSTPSTSASVATSDLAADGSSDAGSVDMPQSIPAPERCLHCPFETQHHEELMQHLQKHACVNPPPPNYQKCAHCDYNANEEAEMEEHTALHFNASEKLKSVEFFTCYDQLEISVEREPEETADSKAEQTEHNNNQDNVMNANVQQQQQPSSNSPDEQPADEGMEESSAKKSSTKLILYKNDGGLSVKLSQEEGANSEFQKSENISDRLRRRILRGSANQTEDATSTSGQERPATPDKMILVNAQTGKVISRK

Target ORF information:

RefSeq Version	XM_016178337.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant G (Dsim\GD22090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016178337.1

ORF Insert Sequence:

ATGGACCTGC CTTCAATGGT GCAGCGTTCA GGAGACACGC TCATCGTGCG CAGCGTGGTC 
AGCGGCAATC AGCTGTATAC GGAGCAGGGT GGCCACCAAC CCGCCAGCGG GAACACGGGA 
CACATGACGA GCAACTCGAC CAGCAATACG CCAGCCGCAC AGGCGGGCTC ATCCCTACTG 
GAGCGACATG TGGAGCGCTT CCGGCTGCAG CAGTTGCTGC AACAACAGCG CCAAGCGGCA 
GCTGCAGTTG CCGTTGTGAA CAGCGTGCAG CAGCAGCAGC AACAGCAGCC ACAGCAGGTT 
GCCATTGGCA TGGATGCCAA GGAGGAGGGC CTGCCGCAGT GCAAGATCAA GCGGAACTAT 
AGCTGCAACC ACTGCGCGTA CTTCACACAG AATCCGCGCT ACCACCTGAC CCACTTGCGC 
GACGTGCACG GCGAGAAGAT AGTGATCAAC AAGTGCAAGC TGTGCCTGTA CGCCTCGCGA 
CATTTCCAGA AGCTGGTGCG GCACATGAAG ATGGTGCACG GCTGCACGGA CGGCATTCCC 
AGTGGTCATG GTCAGGCCAG GGGCAAGAGG GGCATGAGCC GAGAGGCACG CAAGCGGCGA 
CTGGAGGAGA GCGTGGGTGT AATGGGTGGC CAATCCCTGA CAGTCACGGT GCCGGATGTG 
CCCACGCTGG AGCAAGTGAA GCGGGAGCTG CTCCTGCAGG AGGAGAAGCT GCAGCGGGAC 
ATTCAGGCCT TCAACCAGCG ACAGCGCGAG GAGCAGCAGC GCGAACAGCA GCGGGAACTG 
GAACTGGTGG CCACCAGTGC GTATGAGCGA CAAATGCAGG TCCTTCGCGA CTACGAGCGT 
CAGTCGCCCG CCGAACCACC AACTCCTTCG CCCACCAGTG GCTCGGCAAC GCCACCCTCC 
AACGGGGAGG AGCCCCAGAA CCGACTGCTC AAGTGCAGCG CCTGCGAGTT CACCACCCTC 
TATCGCACCC AACTGCGAGC CCACGAGCTG GCCGAGCACG GCAAGACCAA GTTCTTCCGC 
TGCGACAAGT GCAGCTATGT GACCCACATC AAGGCGCGCT TCAGCAAGCA CGTCAAGTAC 
CACTCCATGC CGATGATCAA GTGCGTCACC TGCGACTTCC GCACGCCCTA CAAATGGAAT 
CTGGACAGGC ACATGAAGAA TCACGGCGGT GCGGGAGCCT TTAAATGCGC CGCCTGCGAC 
TTTACCGCCG ACATCAAGCA ATCGCTGACG GTCCACGAGA TGAACCACCA TGTGCCGCCG 
GTGGGCAATG CTGGCTCCAT TTGGCCAAGA CGCCAGAATA AAGTCGGAGC GAGCGAGATG 
TGCGAGGATT TCCTCAGCGA TTCAGCCGAG CTGGAGGATC AATATAACAA CAACAATGTC 
GATGACGAAC TGGACGGCGT CGAGGATGCC GACGACGCAA TGAGCGGAGG GGAGGAGCAG 
CCGATGCACC TCCATCACTA CGGCAAACGG AGCAAGTACG ACGATGAGGA GGAGCCCACA 
GATCTCTCCC AGAAGGGCGG ATGCTCCTCG GACACCTCCA GTGTGGGCAC GCCCACGCCG 
AGGGCACAGC GAACCATGCC CAATCTCATA CCGATCAGCA AGGGGGCCAA AGACGTATTA 
AACCTTTCGA AGGAGACGAA TGCCTCGCGC AGTTCCCTAA CGGAGATTGC CTCCATGTTC 
TTCAATGAGA AACAGATCTC GGAGATGCTT GACAAGTCGG ATGTGCCACA ATTGTCGCCG 
GCAACGACGG TGGCCTCCCA GAACTCGACG CGCAGCCAAA TGACCAAGAA GTCCAGCTTC 
ATGGACAAAC TGAAGACGGG GGCGCAGCAC GAGAATCTGG TGTGCCAGTG CGGTCATGTG 
GCCAAGTGCC TTTCGGAGTC GATCATCCAC GGCAAGAGCT GTCACGCCTC GGCGGTGATC 
ATCGACGAGG ATGATGCTGG ACTGCACGAG GACGATGCCG ACGATCGGCT GGAGATCGAT 
GAGGACGACG AGGACCATCA CTCCCATTCG GCCCTGAATC TCAGTGTGAC GGGATCGACG 
CGTTGCCAGC ACTGCCGGCA CCGCTGCAAG TCCTCCACGG ATCTGATGCA CCATCTGAAG 
CAGTGCGTCG AGGCGATCCG TTGCGCCAAC GAGATGTACG ACTCGAATTC CGGCGAGAGT 
GGCGATCGGC GCCCGGACTC GCAGAGCCTC CAGCAGCAGG CGGCCGTCCA GCAGCAGCGG 
GTGTGCATCT GGAACAAAGC CACCAAGGAG ATTGTCGCCG CAGCAGCCGC ATCCATGCAG 
CAGGAGAAGA ACAACCACAA CCTGGTCAAG TCCCCAACCG GAAGTCAGGC GGCCAGCAAC 
GAGGAGAACA GCTACTACGG CGTAGAAACG GCACCCGGAT ACGGCGAGGT AACCAAAAAG 
ATGACACCGG AGGAAGAGGC AGCCAACTCC TCGCTGAAGA AGGTGTACAA GTGTCCGCAC 
TGCAGCTTCT GGGCCTCCAC GGCGTCCCGC TTCCACGTCC ACATCGTGGG CCATCTGAAC 
AAGAAGCCCT TTGAGTGCTC CCTCTGCTCG TACCGCTCCA ACTGGCGCTG GGACATCACC 
AAGCACATCC GATTGAAGAC GATCCGCGAT CCCTCGCACA AGACGGCCAA GGTTCTGATG 
AACGACGAGA CGGGCCGTAG AAACTACACC AAGTACAACA AATACATTAC CCTGATGAAG 
GTAACCGAGG AGGATGGTGA TCCCAAGCTG ATGAAGTCCG GCGAGATGAC ACCGAACCAG 
GTGGCCTCGT TGGCCTTCCT CAAGGACTAT GCCAAGGTCG GCTCGGTTTC CGGGCAGGAT 
ATCACATTGG AACCAGTGCT TCCCAACAAA CCCAATGTAC TGGACGATGC CCATTTGGCG 
GACAACCTAA TACGGATTCC CTTGCTGGCC ACCATGATGA ATGCCGCTAT GGCGCAACAG 
CAACACCAGC AGCAGCAACA GAAGGAGCAC CAGTCAACGA TGATCACGCC CTCGGTGACC 
ATTTCCCCGG TCAAGCGATC CAATCAGGCA TCCGTGATGC AGGGCAAGCC CAGCGACGAC 
CTGATCACCG AGGTCCACCA GGAGGGCAAT GAGAAGAGGA CCGTCTATCG GTGCCGCAAG 
TGTAACTTCG GGCACCAGAA TCGCGATGCT GTGCTGGCGC ACGTGAAGAT TCACTACCAG 
GACGCCAGCT ACCCGAAGTC CAACTCTGGG AACTCCAGCA CTTCGCCACT GCAGGTATCC 
GTGGGCGGTA GTCCACAATT CTACATGAAC AAGGTCTTTG CGGCCATGTG CCTGTCGCAG 
ACGCAGTCAC AGTCGTCGCC CAACTCCGGC TCCGCCGGAA CGAGTCCGGC CATCTCGGCC 
GGACTGCTGC AGCGGGCCAT CCAGGAGGCC CAGCACTCGC CCACTCCGAA CGCCAGCGCT 
CTGAGCGGAC TTGCTTTGGC GTTGGCGGGC GGAAAGCCAC AAGCCAATAC GACGAAAGCC 
AGTGCCGCCT CCATTTTAGA GCACGGTGAG CAGAAAGCGA GTCAAAACGA GGCAAGTGCC 
GACAGTCTGA CGTCGCCGAA CACCACCACC ACGAACTCAA CCACTAGTAG AACCACCAAA 
GACAACACCG CCAGATCGCT GCAGGATCTG CTCACCTCAC CGCGCAGTGC CAGAAATGGA 
AATCATACTT CTAACGCTAA CAGTAACTCG GTTGTGGGAA ATGGACTCCG GCTGGATGCC 
GTCTACGTCG CCTCATCCAC CAACAATACC ACAACACCAC CACCACCTCC CACCAACTCA 
GTCAGCAAGC CCAATGCATC GCCTTTGCCA GTAACTACCA CTCCTACTCC TATTTCTACT 
GCCACACCGC TGGCTCATCC ACTGGGTGCG GGTAACCACA GATCATCCTC ATCCCATCAC 
AATGACCAAA ATCATGCTCC ACCCTCACAC ATGGCCGGTG ATGGTTGCCA CCTGGCGTCG 
GGTCTAACCC ATGTCCACAC CATAAGCACT AACACGAAAG CCAATCCCAG CTCCAATTCC 
TTTCCCTCGT CATCGTCGTC GTCGTCGTCA TCATCGTCTT CGGTGGCCTC CACTTCATCG 
GCCGCCGGCT CAGCGTCCGC CTCGGCGTCC TCCACCACAT CGTCGCCACC GCCGCCGGCA 
GCCTCATCCT CCGCCTCCGC CTACATGACT GCCATGGCGC CGCAGAAACA TGCGCCAACC 
GTTGCGCAGA TGTCGCATCC GCAGACGAGA TTCCATAGCC ATAGTCCAGG TAGTCCCAGC 
TTGCTGACCA TGGCCGATGG CGATCGACGC GAACCGTCGC CGTATCGCTG CGGCCACTGC 
CACCAGGTCT CGAATTGGAA GCATGTCATC CAGCGGCACT GTCGCTTAAA GCATTCCGGG 
GATATTCGGA TCGAGACCCT TGAGCGAGGA GCCAGTGTCT CGGCGAACGC ACCACCCATC 
TACCGACCCC TGGGAGCAGG TGCCAGCAAC GAATCCCAGT CCCACATCCA CAATCCGTCG 
AATCCCATCA CCAGCAACAT CCTAAAGTCG GGCAGCAACA CGAATGCCAA CAGCAACATC 
AACAGCAATA GTGCCCAGCC CCTGCTGCTG ACCACCAAGC AGCTGGAGCA GATCTTCCAC 
ACACCGCTCT CCGCCAGCGC CGCTGCAGTG GTGAATGCGG CCAACACCCA GCAGGATATC 
CAAGCGGCGG CGGCCTATTG GGCAGCAGCC TGCAAAGCAG TGGTGGCCAA CAGCAGCGCC 
GAGGAGTTGC TGCAGTTGCA GCAGAACGAC CAGATCGAGA TCACCCGCCT GTCGACCACG 
GCGGAGCACT CCATCGGATC CAGCGCCGGC GGCAACAATA CAAAGAGCAA GCAGCAGAAG 
TGTCCCGTCT GTCCGTACAT CTCGGAGAGC AAATCCCAGA TGAACTACCA CGTGTCGCTC 
CACAAGCCCA CGCAGTACGA GTGCCGGCTG TGCACATTTG TGTGTGCCAA GAAACAGCAT 
CTGAGCAGCC ACATGAGAAC GGTGCACCAG CAGCAGATGG TTGCATCGGG AGCAGGTGCA 
GCCGCAGGAG CAGCCGGAGG AGTAGGAGGA GGCGGAGCTT CCTCGCTGGG TCTGGACTTC 
AGTGTGGCCC TGCAGTTGGC CGCAGCTGCC AAGCAGGTGC AACAACTGCC CGCCTCAACA 
CCACTCTCCA TCGATTTGTC CCAGATCCAG ATGGATGCCG CCTCCGATGC GGAGCTCAAT 
CCACAGCAAC TGCCGCCGGA GTACCAATAC AAGCTGATCA GCTACTGTCC CAGGTGTCCG 
GCTCGCTTTG CCCAGAAGCA CAACGACGAG CGCAATGCCA AGCAAGAGCT GGAGCAGCAT 
TTGCTGGCAC ACAGCGACCA GGAGCCGGAG GATCAGGATC CGAGCTATGT CTGCGCCTAC 
TGTGAGTATC GTACATCGGA AGAGACCCTG CTGCAACTGC ACCGTGCGGT GCACATGTCC 
CACTACCAGG AGAAGTGCCA GGAGCTGTAC CGGAACTGCA AGGAGGATGT CGAGTATCCG 
GCACCCAAGT TGCTGCAGCT GACCGGTCCC GAGACCATTT GGGTGGTAGA CAATGAGCTG 
AGCTTGCAGC TGCTGCAGCA AACTACCGGA GGAGCAGTTA GTACCTCCAC CGGTGGGTTT 
GACAACCAGA ACTCTCTGCT CAAGAAGCAG CTGGAATCGG GCGGTGGCCA GATGGTCGCA 
CTTCCACGGG AAGCATCCGT GACCAAAAAT GAGGCGGAAG GCCCCGAGGA GGACGAGGAG 
CGACGTAGCA GCTCCACGCC GTCGACCAGC GCTTCGGTGG CCACCAGTGA TTTGGCCGCA 
GATGGCAGCA GTGATGCAGG ATCCGTTGAC ATGCCCCAGT CCATTCCGGC ACCGGAACGC 
TGTCTCCATT GTCCATTCGA AACGCAGCAT CACGAGGAAC TGATGCAGCA TCTCCAGAAG 
CACGCCTGCG TGAATCCTCC ACCTCCCAAC TACCAGAAAT GTGCCCACTG TGATTACAAT 
GCCAATGAGG AGGCGGAAAT GGAGGAGCAC ACGGCGCTGC ATTTCAATGC CAGTGAGAAG 
CTCAAGTCCG TGGAGTTCTT CACCTGCTAC GATCAGCTGG AGATCAGCGT GGAGCGGGAA 
CCGGAGGAAA CCGCTGACTC AAAGGCGGAG CAGACCGAGC ACAACAACAA CCAGGACAAT 
GTGATGAACG CCAATGTGCA GCAGCAGCAG CAGCCGAGCA GCAACAGCCC AGATGAACAG 
CCAGCTGACG AGGGGATGGA GGAATCGTCG GCCAAGAAGT CCAGCACCAA GTTGATTCTG 
TACAAGAACG ATGGCGGTTT GAGTGTAAAG CTCTCCCAGG AAGAGGGCGC CAATTCAGAG 
TTCCAGAAGA GCGAGAATAT CAGCGATCGC CTGCGCAGAA GGATCCTCCG GGGAAGTGCC 
AACCAGACGG AGGACGCCAC TTCCACCTCC GGCCAGGAGC GACCCGCAAC ACCGGACAAA 
ATGATACTGG TCAATGCCCA GACGGGCAAA GTCATTTCCA GAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Genome research23(1)89-98(2013 Jan)
Hu TT,Eisen MB,Thornton KR,Andolfatto P

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Dsim\GD22090 cDNA ORF clone, Drosophila simulans

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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