Dpse\GA25341 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA25341
Entrez Gene ID	6903333
Full Name	GA25341 gene product from transcript GA25341-RA
Synonyms	GA25341,dpse_GLEANR_16837
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015179769.1	XP_015035255.1	uncharacterized protein, isoform B
XM_002132178.2	XP_002132214.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA25341 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA25341 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe14708	XM_002132178.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA25341), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODe17000	XM_015179769.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA25341), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA25341 ( XM_002132178.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002132214.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA25341), mRNA.

Price & Availability↑

CloneID	ODe14708
Clone ID Related Accession (Same CDS sequence)	XM_002132178.2
Accession Version	XM_002132178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589966). On Dec 22, 2015 this sequence version replaced XM_002132178.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTGTC GTTGCACTCG AAAGCTGACG ATGGCCATGA GCGGCTGTGT GATTATTTTG 
GTGATCGCCC TCACCGTCGG TCTTTGTGTG GGCCTTGGCG GCAGCGAGAG CGAGGGAGTC 
CGCGAGGATC CGCCCAAATT CACAATCGAC CATGAGTCCA ACAGCTTTGT GCTGAATGGC 
GAACCATTCC GATATGTCGC TGGATCGTTC CATTACTTCC GGGCGGTGCC CGAGGCCTGG 
AGGAGTCGAT TGCGGACTAT GAGAGCATCT GGTCTGAATG CAGTGGATAC CTATGTGGAA 
TGGTCGCTCC ACAATCCCCA CGATGGGGTC TACAACTGGG AAGGCATTGC CGATGTTGTG 
AAATTTCTAG AGATTGCCCA AGAGGAAGAC TTTTACATAA TACTGCGTCC TGGTCCCTAT 
ATCTGTGCAG AACGCGATAA TGGTGGCCTT CCCCACTGGC TATTTAAGAA ATATCCCAGC 
ATCAAAATGA GAACTAGTGA TAGCAATTAC ATGGCCGAAG TCGGTAAATG GTATGCAGAA 
TTGATGCCAC GCCTGCAGCA CTTGCTGATC GGCAACGGTG GCAAGATCAT TATGGTGCAG 
GTTGAGAACG AGTACGGCGA CTACGAATGC GATAAGGACT ACCTGAATTG GCTGCGTGAC 
GAGACGGAGA AGTATGTGAA TGGGAATGCC CTGCTCTTCA CAACGGACAT ACCCAACGAA 
CGCATGAGCT GCGGCAAGAT CGATAATGTT TTTGCCACCA CGGACTTTGG CATTGATCGA 
ATTCACGAGA TTGACGACAT CTGGGCAATG CTTCGAAAAC TGCAGCCCAC GGGACCACTG 
GTCAATTCGG AGTTCTATCC AGGATGGTTG ACCCACTGGC AGGAGATGAA TCAGCGACGC 
GATGGTCAAG TGGTGGCCGA TGCCTTGAAG ACCATTCTGA GCTACAATGC CAGCGTCAAT 
CTGTACATGT TCTTTGGAGG CACCAACTTC GGATTCACTG CCGGAGCTAA CTACAATCTC 
GATGGAGGTG TTGGCTATGC GGCAGACATC ACCAGCTATG ACTACGATGC AGTGATGGAC 
GAGGCAGGCG GCGTGACCAG CAAATACAAC CTGGTTAAGC AGGTGATTGG TGAATTTCTG 
CCACTGCCCG AGATCACGCT CAATCCCGCC AAGCGTTTGG CCTACGGCAA GGTGGAAGTG 
ACGCCCTCGC TGGCGCTTCT CTCTGCAGAA GGACGTTCTG CCCTGGCCAA GGGCGACCCT 
GTGGAGTCAA CCAAACCAAA ATCCTTTGAG GAACTCGATC TGTACTCCGG TCTGGTGCTG 
TACGAGACAG AGCTGCCCAG CATGGACCTC GATCCGGCTC TCCTGAAGGT GGACCAGATC 
AACGATCGTG CCCATGTCTT TGTGGATCAA GAGCTGGTAG GAACACTGTC CAGGGACGCG 
CAAATATATT CCCTGCCATT GAGCAAAGGA TGGGGCAGCA CGCTCCAGCT ACTGGTGGAG 
AATCAGGGAC GCGTCAACTT CTACATCTCC AATGACACAA AGGGTATTTT CGGTGAAGCG 
AGTCTCCAGC TGCACAATGG CGGCTATCTC CCGCTAGAGA ACTGGAAGAG CACAGCATTC 
CCCCTCGAAG AGGTCACCGT AGAGGCCTGG CGGAAGAAAA GCGGAGAACG AGCACTGGAT 
GCATTCTTGG CCAGGCAACG AATCCTGCGT AATGGACCCA TCCTCTATAC GGGCAGCCTC 
AAGGTGGTGG AGGTCGGAGA CACCTACCTA AACATGGCTG GCTGGGGCAA GGGCGTGGCC 
TATGTGAATG GCTTCAATCT GGGCCGGTAC TGGCCCGTGG CGGGTCCCCA GGTCACACTC 
TACGTACCCA ACGAGATCCT CAAGGTGGGC GACAACTCTT TGGTGATTCT GGAGTATCAG 
CGGACGAACA AGACCGCAAA TGGTGAGGAT CTACCCGCTG TGCAATTTGA TGCGGTTGCT 
CAATTGGATG GACAGTCCGG AGATGTGCCT CTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002132214.1
CDS	137..2155
Translation	MSCRCTRKLTMAMSGCVIILVIALTVGLCVGLGGSESEGVREDPPKFTIDHESNSFVLNGEPFRYVAGSFHYFRAVPEAWRSRLRTMRASGLNAVDTYVEWSLHNPHDGVYNWEGIADVVKFLEIAQEEDFYIILRPGPYICAERDNGGLPHWLFKKYPSIKMRTSDSNYMAEVGKWYAELMPRLQHLLIGNGGKIIMVQVENEYGDYECDKDYLNWLRDETEKYVNGNALLFTTDIPNERMSCGKIDNVFATTDFGIDRIHEIDDIWAMLRKLQPTGPLVNSEFYPGWLTHWQEMNQRRDGQVVADALKTILSYNASVNLYMFFGGTNFGFTAGANYNLDGGVGYAADITSYDYDAVMDEAGGVTSKYNLVKQVIGEFLPLPEITLNPAKRLAYGKVEVTPSLALLSAEGRSALAKGDPVESTKPKSFEELDLYSGLVLYETELPSMDLDPALLKVDQINDRAHVFVDQELVGTLSRDAQIYSLPLSKGWGSTLQLLVENQGRVNFYISNDTKGIFGEASLQLHNGGYLPLENWKSTAFPLEEVTVEAWRKKSGERALDAFLARQRILRNGPILYTGSLKVVEVGDTYLNMAGWGKGVAYVNGFNLGRYWPVAGPQVTLYVPNEILKVGDNSLVILEYQRTNKTANGEDLPAVQFDAVAQLDGQSGDVPLK

Target ORF information:

RefSeq Version	XM_002132178.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA25341), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002132178.2

ORF Insert Sequence:

ATGAGTTGTC GTTGCACTCG AAAGCTGACG ATGGCCATGA GCGGCTGTGT GATTATTTTG 
GTGATCGCCC TCACCGTCGG TCTTTGTGTG GGCCTTGGCG GCAGCGAGAG CGAGGGAGTC 
CGCGAGGATC CGCCCAAATT CACAATCGAC CATGAGTCCA ACAGCTTTGT GCTGAATGGC 
GAACCATTCC GATATGTCGC TGGATCGTTC CATTACTTCC GGGCGGTGCC CGAGGCCTGG 
AGGAGTCGAT TGCGGACTAT GAGAGCATCT GGTCTGAATG CAGTGGATAC CTATGTGGAA 
TGGTCGCTCC ACAATCCCCA CGATGGGGTC TACAACTGGG AAGGCATTGC CGATGTTGTG 
AAATTTCTAG AGATTGCCCA AGAGGAAGAC TTTTACATAA TACTGCGTCC TGGTCCCTAT 
ATCTGTGCAG AACGCGATAA TGGTGGCCTT CCCCACTGGC TATTTAAGAA ATATCCCAGC 
ATCAAAATGA GAACTAGTGA TAGCAATTAC ATGGCCGAAG TCGGTAAATG GTATGCAGAA 
TTGATGCCAC GCCTGCAGCA CTTGCTGATC GGCAACGGTG GCAAGATCAT TATGGTGCAG 
GTTGAGAACG AGTACGGCGA CTACGAATGC GATAAGGACT ACCTGAATTG GCTGCGTGAC 
GAGACGGAGA AGTATGTGAA TGGGAATGCC CTGCTCTTCA CAACGGACAT ACCCAACGAA 
CGCATGAGCT GCGGCAAGAT CGATAATGTT TTTGCCACCA CGGACTTTGG CATTGATCGA 
ATTCACGAGA TTGACGACAT CTGGGCAATG CTTCGAAAAC TGCAGCCCAC GGGACCACTG 
GTCAATTCGG AGTTCTATCC AGGATGGTTG ACCCACTGGC AGGAGATGAA TCAGCGACGC 
GATGGTCAAG TGGTGGCCGA TGCCTTGAAG ACCATTCTGA GCTACAATGC CAGCGTCAAT 
CTGTACATGT TCTTTGGAGG CACCAACTTC GGATTCACTG CCGGAGCTAA CTACAATCTC 
GATGGAGGTG TTGGCTATGC GGCAGACATC ACCAGCTATG ACTACGATGC AGTGATGGAC 
GAGGCAGGCG GCGTGACCAG CAAATACAAC CTGGTTAAGC AGGTGATTGG TGAATTTCTG 
CCACTGCCCG AGATCACGCT CAATCCCGCC AAGCGTTTGG CCTACGGCAA GGTGGAAGTG 
ACGCCCTCGC TGGCGCTTCT CTCTGCAGAA GGACGTTCTG CCCTGGCCAA GGGCGACCCT 
GTGGAGTCAA CCAAACCAAA ATCCTTTGAG GAACTCGATC TGTACTCCGG TCTGGTGCTG 
TACGAGACAG AGCTGCCCAG CATGGACCTC GATCCGGCTC TCCTGAAGGT GGACCAGATC 
AACGATCGTG CCCATGTCTT TGTGGATCAA GAGCTGGTAG GAACACTGTC CAGGGACGCG 
CAAATATATT CCCTGCCATT GAGCAAAGGA TGGGGCAGCA CGCTCCAGCT ACTGGTGGAG 
AATCAGGGAC GCGTCAACTT CTACATCTCC AATGACACAA AGGGTATTTT CGGTGAAGCG 
AGTCTCCAGC TGCACAATGG CGGCTATCTC CCGCTAGAGA ACTGGAAGAG CACAGCATTC 
CCCCTCGAAG AGGTCACCGT AGAGGCCTGG CGGAAGAAAA GCGGAGAACG AGCACTGGAT 
GCATTCTTGG CCAGGCAACG AATCCTGCGT AATGGACCCA TCCTCTATAC GGGCAGCCTC 
AAGGTGGTGG AGGTCGGAGA CACCTACCTA AACATGGCTG GCTGGGGCAA GGGCGTGGCC 
TATGTGAATG GCTTCAATCT GGGCCGGTAC TGGCCCGTGG CGGGTCCCCA GGTCACACTC 
TACGTACCCA ACGAGATCCT CAAGGTGGGC GACAACTCTT TGGTGATTCT GGAGTATCAG 
CGGACGAACA AGACCGCAAA TGGTGAGGAT CTACCCGCTG TGCAATTTGA TGCGGTTGCT 
CAATTGGATG GACAGTCCGG AGATGTGCCT CTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA25341 ( XM_015179769.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015035255.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA25341), partial mRNA.

Price & Availability↑

CloneID	ODe17000
Clone ID Related Accession (Same CDS sequence)	XM_015179769.1
Accession Version	XM_015179769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589966). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGCGAGCGG CGGGTCTTAA CGCATTAGAT ATCTATGTGG AATGGTCGCT CCACAATCCC 
CACGATGGGG TCTACAACTG GGAAGGCATT GCCGATGTTG TGAAATTTCT AGAGATTGCC 
CAAGAGGAAG ACTTTTACAT AATACTGCGT CCTGGTCCCT ATATCTGTGC AGAACGCGAT 
AATGGTGGCC TTCCCCACTG GCTATTTAAG AAATATCCCA GCATCAAAAT GAGAACTAGT 
GATAGCAATT ACATGGCCGA AGTCGGTAAA TGGTATGCAG AATTGATGCC ACGCCTGCAG 
CACTTGCTGA TCGGCAACGG TGGCAAGATC ATTATGGTGC AGGTTGAGAA CGAGTACGGC 
GACTACGAAT GCGATAAGGA CTACCTGAAT TGGCTGCGTG ACGAGACGGA GAAGTATGTG 
AATGGGAATG CCCTGCTCTT CACAACGGAC ATACCCAACG AACGCATGAG CTGCGGCAAG 
ATCGATAATG TTTTTGCCAC CACGGACTTT GGCATTGATC GAATTCACGA GATTGACGAC 
ATCTGGGCAA TGCTTCGAAA ACTGCAGCCC ACGGGACCAC TGGTCAATTC GGAGTTCTAT 
CCAGGATGGT TGACCCACTG GCAGGAGATG AATCAGCGAC GCGATGGTCA AGTGGTGGCC 
GATGCCTTGA AGACCATTCT GAGCTACAAT GCCAGCGTCA ATCTGTACAT GTTCTTTGGA 
GGCACCAACT TCGGATTCAC TGCCGGAGCT AACTACAATC TCGATGGAGG TGTTGGCTAT 
GCGGCAGACA TCACCAGCTA TGACTACGAT GCAGTGATGG ACGAGGCAGG CGGCGTGACC 
AGCAAATACA ACCTGGTTAA GCAGGTGATT GGTGAATTTC TGCCACTGCC CGAGATCACG 
CTCAATCCCG CCAAGCGTTT GGCCTACGGC AAGGTGGAAG TGACGCCCTC GCTGGCGCTT 
CTCTCTGCAG AAGGACGTTC TGCCCTGGCC AAGGGCGACC CTGTGGAGTC AACCAAACCA 
AAATCCTTTG AGGAACTCGA TCTGTACTCC GGTCTGGTGC TGTACGAGAC AGAGCTGCCC 
AGCATGGACC TCGATCCGGC TCTCCTGAAG GTGGACCAGA TCAACGATCG TGCCCATGTC 
TTTGTGGATC AAGAGCTGGT AGGAACACTG TCCAGGGACG CGCAAATATA TTCCCTGCCA 
TTGAGCAAAG GATGGGGCAG CACGCTCCAG CTACTGGTGG AGAATCAGGG ACGCGTCAAC 
TTCTACATCT CCAATGACAC AAAGGGTATT TTCGGTGAAG CGAGTCTCCA GCTGCACAAT 
GGCGGCTATC TCCCGCTAGA GAACTGGAAG AGCACAGCAT TCCCCCTCGA AGAGGTCACC 
GTAGAGGCCT GGCGGAAGAA AAGCGGAGAA CGAGCACTGG ATGCATTCTT GGCCAGGCAA 
CGAATCCTGC GTAATGGACC CATCCTCTAT ACGGGCAGCC TCAAGGTGGT GGAGGTCGGA 
GACACCTACC TAAACATGGC TGGCTGGGGC AAGGGCGTGG CCTATGTGAA TGGCTTCAAT 
CTGGGCCGGT ACTGGCCCGT GGCGGGTCCC CAGGTCACAC TCTACGTACC CAACGAGATC 
CTCAAGGTGG GCGACAACTC TTTGGTGATT CTGGAGTATC AGCGGACGAA CAAGACCGCA 
AATGGTGAGG ATCTACCCGC TGTGCAATTT GATGCGGTTG CTCAATTGGA TGGACAGTCC 
GGAGATGTGC CTCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015035255.1
CDS	1..1761
Translation	MRAAGLNALDIYVEWSLHNPHDGVYNWEGIADVVKFLEIAQEEDFYIILRPGPYICAERDNGGLPHWLFKKYPSIKMRTSDSNYMAEVGKWYAELMPRLQHLLIGNGGKIIMVQVENEYGDYECDKDYLNWLRDETEKYVNGNALLFTTDIPNERMSCGKIDNVFATTDFGIDRIHEIDDIWAMLRKLQPTGPLVNSEFYPGWLTHWQEMNQRRDGQVVADALKTILSYNASVNLYMFFGGTNFGFTAGANYNLDGGVGYAADITSYDYDAVMDEAGGVTSKYNLVKQVIGEFLPLPEITLNPAKRLAYGKVEVTPSLALLSAEGRSALAKGDPVESTKPKSFEELDLYSGLVLYETELPSMDLDPALLKVDQINDRAHVFVDQELVGTLSRDAQIYSLPLSKGWGSTLQLLVENQGRVNFYISNDTKGIFGEASLQLHNGGYLPLENWKSTAFPLEEVTVEAWRKKSGERALDAFLARQRILRNGPILYTGSLKVVEVGDTYLNMAGWGKGVAYVNGFNLGRYWPVAGPQVTLYVPNEILKVGDNSLVILEYQRTNKTANGEDLPAVQFDAVAQLDGQSGDVPLK

Target ORF information:

RefSeq Version	XM_015179769.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA25341), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015179769.1

ORF Insert Sequence:

ATGCGAGCGG CGGGTCTTAA CGCATTAGAT ATCTATGTGG AATGGTCGCT CCACAATCCC 
CACGATGGGG TCTACAACTG GGAAGGCATT GCCGATGTTG TGAAATTTCT AGAGATTGCC 
CAAGAGGAAG ACTTTTACAT AATACTGCGT CCTGGTCCCT ATATCTGTGC AGAACGCGAT 
AATGGTGGCC TTCCCCACTG GCTATTTAAG AAATATCCCA GCATCAAAAT GAGAACTAGT 
GATAGCAATT ACATGGCCGA AGTCGGTAAA TGGTATGCAG AATTGATGCC ACGCCTGCAG 
CACTTGCTGA TCGGCAACGG TGGCAAGATC ATTATGGTGC AGGTTGAGAA CGAGTACGGC 
GACTACGAAT GCGATAAGGA CTACCTGAAT TGGCTGCGTG ACGAGACGGA GAAGTATGTG 
AATGGGAATG CCCTGCTCTT CACAACGGAC ATACCCAACG AACGCATGAG CTGCGGCAAG 
ATCGATAATG TTTTTGCCAC CACGGACTTT GGCATTGATC GAATTCACGA GATTGACGAC 
ATCTGGGCAA TGCTTCGAAA ACTGCAGCCC ACGGGACCAC TGGTCAATTC GGAGTTCTAT 
CCAGGATGGT TGACCCACTG GCAGGAGATG AATCAGCGAC GCGATGGTCA AGTGGTGGCC 
GATGCCTTGA AGACCATTCT GAGCTACAAT GCCAGCGTCA ATCTGTACAT GTTCTTTGGA 
GGCACCAACT TCGGATTCAC TGCCGGAGCT AACTACAATC TCGATGGAGG TGTTGGCTAT 
GCGGCAGACA TCACCAGCTA TGACTACGAT GCAGTGATGG ACGAGGCAGG CGGCGTGACC 
AGCAAATACA ACCTGGTTAA GCAGGTGATT GGTGAATTTC TGCCACTGCC CGAGATCACG 
CTCAATCCCG CCAAGCGTTT GGCCTACGGC AAGGTGGAAG TGACGCCCTC GCTGGCGCTT 
CTCTCTGCAG AAGGACGTTC TGCCCTGGCC AAGGGCGACC CTGTGGAGTC AACCAAACCA 
AAATCCTTTG AGGAACTCGA TCTGTACTCC GGTCTGGTGC TGTACGAGAC AGAGCTGCCC 
AGCATGGACC TCGATCCGGC TCTCCTGAAG GTGGACCAGA TCAACGATCG TGCCCATGTC 
TTTGTGGATC AAGAGCTGGT AGGAACACTG TCCAGGGACG CGCAAATATA TTCCCTGCCA 
TTGAGCAAAG GATGGGGCAG CACGCTCCAG CTACTGGTGG AGAATCAGGG ACGCGTCAAC 
TTCTACATCT CCAATGACAC AAAGGGTATT TTCGGTGAAG CGAGTCTCCA GCTGCACAAT 
GGCGGCTATC TCCCGCTAGA GAACTGGAAG AGCACAGCAT TCCCCCTCGA AGAGGTCACC 
GTAGAGGCCT GGCGGAAGAA AAGCGGAGAA CGAGCACTGG ATGCATTCTT GGCCAGGCAA 
CGAATCCTGC GTAATGGACC CATCCTCTAT ACGGGCAGCC TCAAGGTGGT GGAGGTCGGA 
GACACCTACC TAAACATGGC TGGCTGGGGC AAGGGCGTGG CCTATGTGAA TGGCTTCAAT 
CTGGGCCGGT ACTGGCCCGT GGCGGGTCCC CAGGTCACAC TCTACGTACC CAACGAGATC 
CTCAAGGTGG GCGACAACTC TTTGGTGATT CTGGAGTATC AGCGGACGAA CAAGACCGCA 
AATGGTGAGG ATCTACCCGC TGTGCAATTT GATGCGGTTG CTCAATTGGA TGGACAGTCC 
GGAGATGTGC CTCTCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA25341 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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