Dpse\GA20218 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20218
Entrez Gene ID	4817198
Full Name	GA20218 gene product from transcript GA20218-RA
Synonyms	GA20218,dpse_GLEANR_1983
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001356609.3	XP_001356645.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20218 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20218 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe17477	XM_001356609.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20218), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20218 ( XM_001356609.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356645.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20218), mRNA.

Price & Availability↑

CloneID	ODe17477
Clone ID Related Accession (Same CDS sequence)	XM_001356609.3
Accession Version	XM_001356609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589964). On Dec 22, 2015 this sequence version replaced XM_001356609.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTCCA ACCCAGGGGG GGAGTCTAAG AATGATGACC TGCCGGCAAA TACCCTGGAA 
TTATTCACAG AGCTGGACCA GGTGCTGCAA ATGATCGAAA ATATCAAATC AATCGATGAC 
GGCTCTTTTG AAAGGGAATT TGAAAACTAC GCCCAAGTGC TGTCCCGCTA CCAAGAGCAA 
CCGCATTTGC TAGATCCCCA TCTAGAGCTG CTGCTGGGTC GCTTGCTGGA CAAGATTCGG 
CTGCAGCAGG CGCCGCCGGG TGAACGTGAT GCAGCCTTCA AGTATCTGTA CATAATTTGC 
AAAGTACGCA CATATAAAGT TCTGGTCAAG TTTATGCCCC ACGAGCTGAG CGATCTGGAG 
TTTGTGCTGG ATCTGTTGGG CCAACAGGAT CCAACTGAAT TTGAGAAATG GGAGACCCGA 
TACATACTCC TACTCTGGAT GTCCATTGTG GTCCTGAATC CCTTTCATAT GTCACGACTG 
GATGCATATG AAACGTCGCC CAGTGTCGCC GTCAACTGCG TGCTGGCCAA CAATGTTGAG 
TCCCACAAAT CCACCAAAAT GGAGCGCATC TTTGAGCTAT GCAAGCTGTA TGTCTCCACC 
AACGACACGT GCAGCAGCAT GGCCGCCTAT CTGGCCGCCA AGTTTTTCGT ACGCATCGAC 
ATCAAGGATC TGTATTTGGA GCGTTTTCTC GACTGGATCA TTGGCCAGCA TCAGGCGGAC 
ACAATGAATG TTAAATTTGG CCAACTGGCC GCCGTTGCGG CCATTCTGAA GCATGGCAAG 
CGTGAGGATT TGCTGCCCTA TGCGGAGAAG CTCCTGCGCT GGATAACATC CTGCCAGTAC 
AAGGAGGGCA ATGATTTTCT CAAGTACAAG TTCTATGTGA AAATTGTCCA GAGATTGGGC 
CTGGTGCACT TGAAGCCACG CATTGCCAGC TGGCGCTACA AAAGAGGCAC ACGTTCGTTG 
GCCACAAATC TCAATCAGCA TCCGGGTTCG GCTGGCGCTC AGTCGACTGC TGCTCTCGAT 
GGCGCCGATG GAGATGCTGG AGAGGAAATT GTTGTTCCCG ATGCCATTGA GGAAGTGATT 
GAGGAGCTGC TCCAGGGCCT GCGCAGTGGC GGCAACGACA TTCGATGGAG TGCCGCCAAG 
GGCTTGGGCC GGGTGACCAA TCGTCTGCCC AAAGAGCTGG CCGACGAGGT GATTGGCTCT 
GTGATTGATA TTCTAAACCC CCTGGAGCCG CACGAGGCTT GGCATGGCGG CTGTTTGGCT 
TTGGCCGAGC TAGCCAAACG TGGTCTGCTG CTACCCCATC GTCTCGAGGA GCTGGTGCCG 
TTGCTCATGC AGGCCTTGTT CTATGACGAA ATGAAGGGCT ACATGTCCGT GGGTCAGCAT 
ATACGCGATG CCGCCTGCTA TATGTGCTGG GCCTTTGCTC GTGCCTACAA TCCCGATGAT 
GTCAAGCCGT TTGTCCAGAA GATCTCTTCA GGCCTGCTCA CGGTGGCGGT GTTTGATCGC 
GAAGTAAACT GCCGCAGAGC TGCTTCAGCC GCTTTCCAAG AAAGCGTTGG CCGTTTGGGC 
AACTTTCCCT TTGGCATCGA GATCTCGGTA ACCACGGACT TTTTCTCTGT GGGCATACGC 
CAGAATTCCT ATCTGAATAT CAGCGATTTC ATAGCACAAT ACGAGGTATA TCGCGAGCCA 
CTAATCACTC ATCTGGTGCA GCACAAAGTG GGCCACTGGG ATCCGGCCAT ACGCGAACTA 
ACAGCCAAGG CGCTGCACAA ACTAACGCTT TGGGAGCCAG AGTATATGGC GGCCGTTGTG 
CTGCCTCAAC TCCTGGCCAA AACGGACACC ATCGACATCA ACAGTCGGCA TGGTTGTGTC 
CTGGCCATGG GTGAAATCAC ACTGGCCGTC CGCAAGCTGG AAATGGCCAG TGATCCAGCC 
GTTGTGTATA TCTCCAATCA ACGGATTGTG GAGCTGAACG AGCTGCTAAA GAATTTCCTC 
ATGAAGAACT ATTACCGCGG TATGAGCGGG GAGCTGCTGA AATCCAGCAC AGCCGATTTC 
ATACGCAACT GCAGTCTGGC CAAGCTGCAG GTGACGCCTG ATTGTCTGCT CTCCTGGCAA 
ACGGTCATTG ACTTCTGTTT GGTGACCAAA AACCCTGTCA TACGAGCCTC TGGTGTGGAG 
GCCTTTGCCG AGCTGTGTCG TTCCTACTAC TGTGTGGATA GTCGCAGCGA CCAGAACGAG 
GCCATCATCA GGTCGTATCT TAAGGGCGCC GAAAACGATC TGGACGAGCA CATACGCATG 
GGCTATATAG CAGCTTTGGG GGTACTGCCC TCGTTTATGC TGAGCCCTCA TCTGTCCCCT 
GTGCTGGAGA GTTTAGTAAA GCACTCGCTG ACACCGCTGC AGGCCGTCCT GGCCGGGGAT 
GTGGGGCAGC AGGAGGAGCA TATCCAGACC TACCGGTGGA GCGAGGCGCG CATGGAAAGC 
ATCAAAGCCT TAACAAAGGT GGTCAAAACT GTGGGCTACG AAGGAACTAT TGATACCTTT 
TCAGATCATG GAAATTTCAA CAAAGTAATC GCTTGCCTGC TGCGAGCCCT GGATGAATAT 
ACCCTGGACA ATCGTGGCGA TATTGGAGCC TGGGTGCGTG AGGCATCCAT GTCATCGCTC 
TACCAGATAG TAACCCAGTG TCCACCGGCT ATGCTAACGG CCACGCATGT CCATGAGATT 
GTCGTGGGCT TCATGCAGCA GGCGGTGGAG AAAATCGATC GTACGCGTGG CCTGGCCGGA 
CGTCTCTGTT GCCAGCTAAT CCATGCTGAG CCACGCATCC CCCATATACG AGAGCATGCG 
AAACTCATTG AGATATTCCC CACGGATGTG AATTCTGTTC TCTGGCTCTT TGCCGATCAT 
ACATTCCCCT TGTTCTGCCA GCTGTTGGGC CTGCCGGATT ACTCCAAGCG TGTCCTGTTG 
GGTCTAACCG CCAGCATTGG GCAGCTGACT GAATCTCTGA TCAAATATGC ATCGACGGCT 
TTATTCCAAT TTTTACGTTC CAATCCCACG ATGGTGCCGC GTCTGTGCAA GGAGATTGTG 
GAGATCTTCC AGGAGAATCT CTTGAACGAG CGCGTGACCT ATCCAATGTT GAGTTTCCTG 
GACATACTCA TTGGTTCCGG CACCACTGAC ATGGTCCTGC ATGACGAATC GAATTCCTTT 
GTCGAGGACA TCTATCGCCT GCTCAATCTG GAGGTCAAGG GCTACAAAAA GTTGTACAAG 
ACAGCCAGCA GCATAGGTGC CTTTTGCCAG CTCATCCAAG TGCCGCGCCT CAGCAAGCGT 
GTGCTCTCCA AACTGTCAGT ATTCTTGGGC CTGCAGCATG TCCATGTCCG CAAAACTGCC 
GCCACCAAGC TCTACGAAGC ATTGGCCCTG CATGGCGATG TTACCGATAT ACCCGAGGAT 
AATATGGATG AAATCCTGAC AATCCTCTCG GAGACAGACT GGACTATGCC CCTGGTGGAG 
GCGCGTCCGT TGCGGAATCA GCTATGCCAT TTGATGGGAA TCAAGCCACC AGTTAGTGGT 
GCAGCAGCAG CTGTAGCTGC CGCCTCTGAA GCAGCAGATA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356645.2
CDS	100..3684
Translation	MMSNPGGESKNDDLPANTLELFTELDQVLQMIENIKSIDDGSFEREFENYAQVLSRYQEQPHLLDPHLELLLGRLLDKIRLQQAPPGERDAAFKYLYIICKVRTYKVLVKFMPHELSDLEFVLDLLGQQDPTEFEKWETRYILLLWMSIVVLNPFHMSRLDAYETSPSVAVNCVLANNVESHKSTKMERIFELCKLYVSTNDTCSSMAAYLAAKFFVRIDIKDLYLERFLDWIIGQHQADTMNVKFGQLAAVAAILKHGKREDLLPYAEKLLRWITSCQYKEGNDFLKYKFYVKIVQRLGLVHLKPRIASWRYKRGTRSLATNLNQHPGSAGAQSTAALDGADGDAGEEIVVPDAIEEVIEELLQGLRSGGNDIRWSAAKGLGRVTNRLPKELADEVIGSVIDILNPLEPHEAWHGGCLALAELAKRGLLLPHRLEELVPLLMQALFYDEMKGYMSVGQHIRDAACYMCWAFARAYNPDDVKPFVQKISSGLLTVAVFDREVNCRRAASAAFQESVGRLGNFPFGIEISVTTDFFSVGIRQNSYLNISDFIAQYEVYREPLITHLVQHKVGHWDPAIRELTAKALHKLTLWEPEYMAAVVLPQLLAKTDTIDINSRHGCVLAMGEITLAVRKLEMASDPAVVYISNQRIVELNELLKNFLMKNYYRGMSGELLKSSTADFIRNCSLAKLQVTPDCLLSWQTVIDFCLVTKNPVIRASGVEAFAELCRSYYCVDSRSDQNEAIIRSYLKGAENDLDEHIRMGYIAALGVLPSFMLSPHLSPVLESLVKHSLTPLQAVLAGDVGQQEEHIQTYRWSEARMESIKALTKVVKTVGYEGTIDTFSDHGNFNKVIACLLRALDEYTLDNRGDIGAWVREASMSSLYQIVTQCPPAMLTATHVHEIVVGFMQQAVEKIDRTRGLAGRLCCQLIHAEPRIPHIREHAKLIEIFPTDVNSVLWLFADHTFPLFCQLLGLPDYSKRVLLGLTASIGQLTESLIKYASTALFQFLRSNPTMVPRLCKEIVEIFQENLLNERVTYPMLSFLDILIGSGTTDMVLHDESNSFVEDIYRLLNLEVKGYKKLYKTASSIGAFCQLIQVPRLSKRVLSKLSVFLGLQHVHVRKTAATKLYEALALHGDVTDIPEDNMDEILTILSETDWTMPLVEARPLRNQLCHLMGIKPPVSGAAAAVAAASEAADK

Target ORF information:

RefSeq Version	XM_001356609.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20218), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001356609.3

ORF Insert Sequence:

ATGATGTCCA ACCCAGGGGG GGAGTCTAAG AATGATGACC TGCCGGCAAA TACCCTGGAA 
TTATTCACAG AGCTGGACCA GGTGCTGCAA ATGATCGAAA ATATCAAATC AATCGATGAC 
GGCTCTTTTG AAAGGGAATT TGAAAACTAC GCCCAAGTGC TGTCCCGCTA CCAAGAGCAA 
CCGCATTTGC TAGATCCCCA TCTAGAGCTG CTGCTGGGTC GCTTGCTGGA CAAGATTCGG 
CTGCAGCAGG CGCCGCCGGG TGAACGTGAT GCAGCCTTCA AGTATCTGTA CATAATTTGC 
AAAGTACGCA CATATAAAGT TCTGGTCAAG TTTATGCCCC ACGAGCTGAG CGATCTGGAG 
TTTGTGCTGG ATCTGTTGGG CCAACAGGAT CCAACTGAAT TTGAGAAATG GGAGACCCGA 
TACATACTCC TACTCTGGAT GTCCATTGTG GTCCTGAATC CCTTTCATAT GTCACGACTG 
GATGCATATG AAACGTCGCC CAGTGTCGCC GTCAACTGCG TGCTGGCCAA CAATGTTGAG 
TCCCACAAAT CCACCAAAAT GGAGCGCATC TTTGAGCTAT GCAAGCTGTA TGTCTCCACC 
AACGACACGT GCAGCAGCAT GGCCGCCTAT CTGGCCGCCA AGTTTTTCGT ACGCATCGAC 
ATCAAGGATC TGTATTTGGA GCGTTTTCTC GACTGGATCA TTGGCCAGCA TCAGGCGGAC 
ACAATGAATG TTAAATTTGG CCAACTGGCC GCCGTTGCGG CCATTCTGAA GCATGGCAAG 
CGTGAGGATT TGCTGCCCTA TGCGGAGAAG CTCCTGCGCT GGATAACATC CTGCCAGTAC 
AAGGAGGGCA ATGATTTTCT CAAGTACAAG TTCTATGTGA AAATTGTCCA GAGATTGGGC 
CTGGTGCACT TGAAGCCACG CATTGCCAGC TGGCGCTACA AAAGAGGCAC ACGTTCGTTG 
GCCACAAATC TCAATCAGCA TCCGGGTTCG GCTGGCGCTC AGTCGACTGC TGCTCTCGAT 
GGCGCCGATG GAGATGCTGG AGAGGAAATT GTTGTTCCCG ATGCCATTGA GGAAGTGATT 
GAGGAGCTGC TCCAGGGCCT GCGCAGTGGC GGCAACGACA TTCGATGGAG TGCCGCCAAG 
GGCTTGGGCC GGGTGACCAA TCGTCTGCCC AAAGAGCTGG CCGACGAGGT GATTGGCTCT 
GTGATTGATA TTCTAAACCC CCTGGAGCCG CACGAGGCTT GGCATGGCGG CTGTTTGGCT 
TTGGCCGAGC TAGCCAAACG TGGTCTGCTG CTACCCCATC GTCTCGAGGA GCTGGTGCCG 
TTGCTCATGC AGGCCTTGTT CTATGACGAA ATGAAGGGCT ACATGTCCGT GGGTCAGCAT 
ATACGCGATG CCGCCTGCTA TATGTGCTGG GCCTTTGCTC GTGCCTACAA TCCCGATGAT 
GTCAAGCCGT TTGTCCAGAA GATCTCTTCA GGCCTGCTCA CGGTGGCGGT GTTTGATCGC 
GAAGTAAACT GCCGCAGAGC TGCTTCAGCC GCTTTCCAAG AAAGCGTTGG CCGTTTGGGC 
AACTTTCCCT TTGGCATCGA GATCTCGGTA ACCACGGACT TTTTCTCTGT GGGCATACGC 
CAGAATTCCT ATCTGAATAT CAGCGATTTC ATAGCACAAT ACGAGGTATA TCGCGAGCCA 
CTAATCACTC ATCTGGTGCA GCACAAAGTG GGCCACTGGG ATCCGGCCAT ACGCGAACTA 
ACAGCCAAGG CGCTGCACAA ACTAACGCTT TGGGAGCCAG AGTATATGGC GGCCGTTGTG 
CTGCCTCAAC TCCTGGCCAA AACGGACACC ATCGACATCA ACAGTCGGCA TGGTTGTGTC 
CTGGCCATGG GTGAAATCAC ACTGGCCGTC CGCAAGCTGG AAATGGCCAG TGATCCAGCC 
GTTGTGTATA TCTCCAATCA ACGGATTGTG GAGCTGAACG AGCTGCTAAA GAATTTCCTC 
ATGAAGAACT ATTACCGCGG TATGAGCGGG GAGCTGCTGA AATCCAGCAC AGCCGATTTC 
ATACGCAACT GCAGTCTGGC CAAGCTGCAG GTGACGCCTG ATTGTCTGCT CTCCTGGCAA 
ACGGTCATTG ACTTCTGTTT GGTGACCAAA AACCCTGTCA TACGAGCCTC TGGTGTGGAG 
GCCTTTGCCG AGCTGTGTCG TTCCTACTAC TGTGTGGATA GTCGCAGCGA CCAGAACGAG 
GCCATCATCA GGTCGTATCT TAAGGGCGCC GAAAACGATC TGGACGAGCA CATACGCATG 
GGCTATATAG CAGCTTTGGG GGTACTGCCC TCGTTTATGC TGAGCCCTCA TCTGTCCCCT 
GTGCTGGAGA GTTTAGTAAA GCACTCGCTG ACACCGCTGC AGGCCGTCCT GGCCGGGGAT 
GTGGGGCAGC AGGAGGAGCA TATCCAGACC TACCGGTGGA GCGAGGCGCG CATGGAAAGC 
ATCAAAGCCT TAACAAAGGT GGTCAAAACT GTGGGCTACG AAGGAACTAT TGATACCTTT 
TCAGATCATG GAAATTTCAA CAAAGTAATC GCTTGCCTGC TGCGAGCCCT GGATGAATAT 
ACCCTGGACA ATCGTGGCGA TATTGGAGCC TGGGTGCGTG AGGCATCCAT GTCATCGCTC 
TACCAGATAG TAACCCAGTG TCCACCGGCT ATGCTAACGG CCACGCATGT CCATGAGATT 
GTCGTGGGCT TCATGCAGCA GGCGGTGGAG AAAATCGATC GTACGCGTGG CCTGGCCGGA 
CGTCTCTGTT GCCAGCTAAT CCATGCTGAG CCACGCATCC CCCATATACG AGAGCATGCG 
AAACTCATTG AGATATTCCC CACGGATGTG AATTCTGTTC TCTGGCTCTT TGCCGATCAT 
ACATTCCCCT TGTTCTGCCA GCTGTTGGGC CTGCCGGATT ACTCCAAGCG TGTCCTGTTG 
GGTCTAACCG CCAGCATTGG GCAGCTGACT GAATCTCTGA TCAAATATGC ATCGACGGCT 
TTATTCCAAT TTTTACGTTC CAATCCCACG ATGGTGCCGC GTCTGTGCAA GGAGATTGTG 
GAGATCTTCC AGGAGAATCT CTTGAACGAG CGCGTGACCT ATCCAATGTT GAGTTTCCTG 
GACATACTCA TTGGTTCCGG CACCACTGAC ATGGTCCTGC ATGACGAATC GAATTCCTTT 
GTCGAGGACA TCTATCGCCT GCTCAATCTG GAGGTCAAGG GCTACAAAAA GTTGTACAAG 
ACAGCCAGCA GCATAGGTGC CTTTTGCCAG CTCATCCAAG TGCCGCGCCT CAGCAAGCGT 
GTGCTCTCCA AACTGTCAGT ATTCTTGGGC CTGCAGCATG TCCATGTCCG CAAAACTGCC 
GCCACCAAGC TCTACGAAGC ATTGGCCCTG CATGGCGATG TTACCGATAT ACCCGAGGAT 
AATATGGATG AAATCCTGAC AATCCTCTCG GAGACAGACT GGACTATGCC CCTGGTGGAG 
GCGCGTCCGT TGCGGAATCA GCTATGCCAT TTGATGGGAA TCAAGCCACC AGTTAGTGGT 
GCAGCAGCAG CTGTAGCTGC CGCCTCTGAA GCAGCAGATA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20218 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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