Dpse\GA12516 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA12516
Entrez Gene ID	4816123
Full Name	GA12516 gene product from transcript GA12516-RB
Synonyms	GA12516,dpse_GLEANR_7355
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015181436.1	XP_015036922.1	uncharacterized protein, isoform B
XM_001355742.3	XP_001355778.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA12516 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA12516 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe10427	XM_001355742.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA12516), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe18843	XM_015181436.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA12516), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA12516 ( XM_001355742.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001355778.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA12516), mRNA.

Price & Availability↑

CloneID	ODe10427
Clone ID Related Accession (Same CDS sequence)	XM_001355742.3
Accession Version	XM_001355742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589962). On Dec 22, 2015 this sequence version replaced XM_001355742.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAGA AAAGCAATGT CTGGCAGTTT TTCTACAAGG CCAACGATAC GGTGGCCACA 
TGCTGTCTAT GCAACAGAAA TTACTCGAGA AAGGGCAGAG GCACCACCTG CCTGAGGAAC 
CATTTGAAAA GCAAGCACCC TGTGGAGTTT CAAACACTCG CCGAAGATAT CAGGTGCTTA 
GTGAAAAGAG AAGTAACTTA CAGGTTACCA CAGCATCCTA CATCGCAATT GGAACTGAAA 
CTGCATAAGA TCGCTCCTGA TCCCTTGGAA ATGGACAATG AAGATCAGTT GTTTGCGCGC 
TGTGACGAAC ATTTGGCGCA AATGTTTCTC AATCACTCTT TCGACTTGAT AGAAGGCCCC 
GGATTCATAA ACTTTGTGAA AGTTTTGCAC TCGGGATATG TGATCAAAGC GAGGGGCTAC 
TATGAGAATA TATTGTGCCG CGATATCCAC AATAGACTTC ACGTTCAGGT GAAGCAGCAG 
GTGGATCTGC TAGGTGCTAT ATCGCTGAGC ACCAGTCTGT GGTGGGCCAA TAACGATGGC 
CTGCTTAGCC TTTCGTGTTT TGGTGTCACG AGTGATTTCA AGGCTCAGCG ACTTACGCTC 
AAATGCGAGG CCCTTAGCTA TGAGGACACC GACAGAGTGG CCTATTGTAT TCAGGGTTTG 
ATCTCAGGTT TGGATATGGG GCTTTCTAGA GAGAAGGTTC ATTGCATTAT CCGAGATGAG 
GCAACTGCTC TGGCCGGAGC ACTTCCCGAC TGCTCTGTCT CACGCCTGCA AATGTGTGTG 
AGATTCGCCT TGCAAGAGAA CCAACAGCTC CAGAATCTCT ACTCGAAATG CAAGCAAATT 
GTTGAGCATT TTGCGTCCTC CACGATGGCC CAGGAGCAGC TGAAATTCAT TCAAGAGATT 
CGCCTTAAAC AAGAGCCGTG CTCAATCATC GCCGAAAGTC CCTTGAGATG GAACTCCATT 
TTCGACATTA TGGGCCGTGT GCTCAAGATG AAGGACGCTC TCTCGCTGTA CGCCGAGGAA 
AATAGTCTGG TCCAAATCTA TCCAGATGAA TGGCTGGACA TTGATTTGTG CAAGAAAGTA 
ATGCAGCCTT GCGAGGAGAT CATCAAAATG TGGAGCGATT CTTCAACGAC CGCCTCTTCG 
GTGATTCCGC TTGTGGCTGC CTTGAGGGAT TCTCTTCGAA CAGATGTCCA CAATTTTGTG 
TCCTCAGTGA CCATTTGCAG CTTTGCCCGT AAACTGCTAG AAGAACTGGA AATGAAGTTC 
TCTCATATTA CCACAGATAT AAAATATCTG GTGGCCACAT ATTTGGATCC GCGCTACAAG 
CAAGCCTTCT TCAACGATCG CGAAGAGCAA CTGGTCACCA ACGAAGTGCT GCAGCAGCTA 
ACCAGCATCC AAGGAAACGA CGAGGGTCAG CCTTCTTTGC AAAAAGTTAA AATATCTTCT 
TCGCAAATTA AAAATGAATC AAAGATTGAT ACCATTTTGG ATAATATACT GGCCTCTGGC 
TCTGCCCATA ATCAGACAGC CACCACATCC TTTAGCTCTC AGTCGCAGTT TAAGAACCTC 
CTATATCTAT ACAACTCCGA GCCAAGAATT GAGAGGAGCA TGGATCCTTT GATTTGGTGG 
AAAACAAACA TCAAATACAG CCCCCTGTAT AGGATTGTTC GGCAGTTTCT TTCCGCTCCG 
GCAGCGAGTG CGTCCAACGA GGCATTGTTT GGCGAATCTG CACATTTGTA TAGTGATATG 
CAGGGGCATT TGAGTCCCGA AAGTGTATCC AAAATATTGT TCATCAAGTC CAACTCTAAG 
GTCTCCGACA ACAGAGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001355778.2
CDS	89..1909
Translation	MSKKSNVWQFFYKANDTVATCCLCNRNYSRKGRGTTCLRNHLKSKHPVEFQTLAEDIRCLVKREVTYRLPQHPTSQLELKLHKIAPDPLEMDNEDQLFARCDEHLAQMFLNHSFDLIEGPGFINFVKVLHSGYVIKARGYYENILCRDIHNRLHVQVKQQVDLLGAISLSTSLWWANNDGLLSLSCFGVTSDFKAQRLTLKCEALSYEDTDRVAYCIQGLISGLDMGLSREKVHCIIRDEATALAGALPDCSVSRLQMCVRFALQENQQLQNLYSKCKQIVEHFASSTMAQEQLKFIQEIRLKQEPCSIIAESPLRWNSIFDIMGRVLKMKDALSLYAEENSLVQIYPDEWLDIDLCKKVMQPCEEIIKMWSDSSTTASSVIPLVAALRDSLRTDVHNFVSSVTICSFARKLLEELEMKFSHITTDIKYLVATYLDPRYKQAFFNDREEQLVTNEVLQQLTSIQGNDEGQPSLQKVKISSSQIKNESKIDTILDNILASGSAHNQTATTSFSSQSQFKNLLYLYNSEPRIERSMDPLIWWKTNIKYSPLYRIVRQFLSAPAASASNEALFGESAHLYSDMQGHLSPESVSKILFIKSNSKVSDNRV

Target ORF information:

RefSeq Version	XM_001355742.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA12516), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355742.3

ORF Insert Sequence:

ATGAGCAAGA AAAGCAATGT CTGGCAGTTT TTCTACAAGG CCAACGATAC GGTGGCCACA 
TGCTGTCTAT GCAACAGAAA TTACTCGAGA AAGGGCAGAG GCACCACCTG CCTGAGGAAC 
CATTTGAAAA GCAAGCACCC TGTGGAGTTT CAAACACTCG CCGAAGATAT CAGGTGCTTA 
GTGAAAAGAG AAGTAACTTA CAGGTTACCA CAGCATCCTA CATCGCAATT GGAACTGAAA 
CTGCATAAGA TCGCTCCTGA TCCCTTGGAA ATGGACAATG AAGATCAGTT GTTTGCGCGC 
TGTGACGAAC ATTTGGCGCA AATGTTTCTC AATCACTCTT TCGACTTGAT AGAAGGCCCC 
GGATTCATAA ACTTTGTGAA AGTTTTGCAC TCGGGATATG TGATCAAAGC GAGGGGCTAC 
TATGAGAATA TATTGTGCCG CGATATCCAC AATAGACTTC ACGTTCAGGT GAAGCAGCAG 
GTGGATCTGC TAGGTGCTAT ATCGCTGAGC ACCAGTCTGT GGTGGGCCAA TAACGATGGC 
CTGCTTAGCC TTTCGTGTTT TGGTGTCACG AGTGATTTCA AGGCTCAGCG ACTTACGCTC 
AAATGCGAGG CCCTTAGCTA TGAGGACACC GACAGAGTGG CCTATTGTAT TCAGGGTTTG 
ATCTCAGGTT TGGATATGGG GCTTTCTAGA GAGAAGGTTC ATTGCATTAT CCGAGATGAG 
GCAACTGCTC TGGCCGGAGC ACTTCCCGAC TGCTCTGTCT CACGCCTGCA AATGTGTGTG 
AGATTCGCCT TGCAAGAGAA CCAACAGCTC CAGAATCTCT ACTCGAAATG CAAGCAAATT 
GTTGAGCATT TTGCGTCCTC CACGATGGCC CAGGAGCAGC TGAAATTCAT TCAAGAGATT 
CGCCTTAAAC AAGAGCCGTG CTCAATCATC GCCGAAAGTC CCTTGAGATG GAACTCCATT 
TTCGACATTA TGGGCCGTGT GCTCAAGATG AAGGACGCTC TCTCGCTGTA CGCCGAGGAA 
AATAGTCTGG TCCAAATCTA TCCAGATGAA TGGCTGGACA TTGATTTGTG CAAGAAAGTA 
ATGCAGCCTT GCGAGGAGAT CATCAAAATG TGGAGCGATT CTTCAACGAC CGCCTCTTCG 
GTGATTCCGC TTGTGGCTGC CTTGAGGGAT TCTCTTCGAA CAGATGTCCA CAATTTTGTG 
TCCTCAGTGA CCATTTGCAG CTTTGCCCGT AAACTGCTAG AAGAACTGGA AATGAAGTTC 
TCTCATATTA CCACAGATAT AAAATATCTG GTGGCCACAT ATTTGGATCC GCGCTACAAG 
CAAGCCTTCT TCAACGATCG CGAAGAGCAA CTGGTCACCA ACGAAGTGCT GCAGCAGCTA 
ACCAGCATCC AAGGAAACGA CGAGGGTCAG CCTTCTTTGC AAAAAGTTAA AATATCTTCT 
TCGCAAATTA AAAATGAATC AAAGATTGAT ACCATTTTGG ATAATATACT GGCCTCTGGC 
TCTGCCCATA ATCAGACAGC CACCACATCC TTTAGCTCTC AGTCGCAGTT TAAGAACCTC 
CTATATCTAT ACAACTCCGA GCCAAGAATT GAGAGGAGCA TGGATCCTTT GATTTGGTGG 
AAAACAAACA TCAAATACAG CCCCCTGTAT AGGATTGTTC GGCAGTTTCT TTCCGCTCCG 
GCAGCGAGTG CGTCCAACGA GGCATTGTTT GGCGAATCTG CACATTTGTA TAGTGATATG 
CAGGGGCATT TGAGTCCCGA AAGTGTATCC AAAATATTGT TCATCAAGTC CAACTCTAAG 
GTCTCCGACA ACAGAGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA12516 ( XM_015181436.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015036922.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA12516), mRNA.

Price & Availability↑

CloneID	ODe18843
Clone ID Related Accession (Same CDS sequence)	XM_015181436.1
Accession Version	XM_015181436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589962). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAGA AAAGCAATGT CTGGCAGTTT TTCTACAAGG CCAACGATAC GGTGGCCACA 
TGCTGTCTAT GCAACAGAAA TTACTCGAGA AAGGGCAGAG GCACCACCTG CCTGAGGAAC 
CATTTGAAAA GCAAGCACCC TGTGGAGTTT CAAACACTCG CCGAAGATAT CAGGTGCTTA 
GTGAAAAGAG AAGTAACTTA CAGGTTACCA CAGCATCCTA CATCGCAATT GGAACTGAAA 
CTGCATATCG CTCCTGATCC CTTGGAAATG GACAATGAAG ATCAGTTGTT TGCGCGCTGT 
GACGAACATT TGGCGCAAAT GTTTCTCAAT CACTCTTTCG ACTTGATAGA AGGCCCCGGA 
TTCATAAACT TTGTGAAAGT TTTGCACTCG GGATATGTGA TCAAAGCGAG GGGCTACTAT 
GAGAATATAT TGTGCCGCGA TATCCACAAT AGACTTCACG TTCAGGTGAA GCAGCAGGTG 
GATCTGCTAG GTGCTATATC GCTGAGCACC AGTCTGTGGT GGGCCAATAA CGATGGCCTG 
CTTAGCCTTT CGTGTTTTGG TGTCACGAGT GATTTCAAGG CTCAGCGACT TACGCTCAAA 
TGCGAGGCCC TTAGCTATGA GGACACCGAC AGAGTGGCCT ATTGTATTCA GGGTTTGATC 
TCAGGTTTGG ATATGGGGCT TTCTAGAGAG AAGGTTCATT GCATTATCCG AGATGAGGCA 
ACTGCTCTGG CCGGAGCACT TCCCGACTGC TCTGTCTCAC GCCTGCAAAT GTGTGTGAGA 
TTCGCCTTGC AAGAGAACCA ACAGCTCCAG AATCTCTACT CGAAATGCAA GCAAATTGTT 
GAGCATTTTG CGTCCTCCAC GATGGCCCAG GAGCAGCTGA AATTCATTCA AGAGATTCGC 
CTTAAACAAG AGCCGTGCTC AATCATCGCC GAAAGTCCCT TGAGATGGAA CTCCATTTTC 
GACATTATGG GCCGTGTGCT CAAGATGAAG GACGCTCTCT CGCTGTACGC CGAGGAAAAT 
AGTCTGGTCC AAATCTATCC AGATGAATGG CTGGACATTG ATTTGTGCAA GAAAGTAATG 
CAGCCTTGCG AGGAGATCAT CAAAATGTGG AGCGATTCTT CAACGACCGC CTCTTCGGTG 
ATTCCGCTTG TGGCTGCCTT GAGGGATTCT CTTCGAACAG ATGTCCACAA TTTTGTGTCC 
TCAGTGACCA TTTGCAGCTT TGCCCGTAAA CTGCTAGAAG AACTGGAAAT GAAGTTCTCT 
CATATTACCA CAGATATAAA ATATCTGGTG GCCACATATT TGGATCCGCG CTACAAGCAA 
GCCTTCTTCA ACGATCGCGA AGAGCAACTG GTCACCAACG AAGTGCTGCA GCAGCTAACC 
AGCATCCAAG GAAACGACGA GGGTCAGCCT TCTTTGCAAA AAGTTAAAAT ATCTTCTTCG 
CAAATTAAAA ATGAATCAAA GATTGATACC ATTTTGGATA ATATACTGGC CTCTGGCTCT 
GCCCATAATC AGACAGCCAC CACATCCTTT AGCTCTCAGT CGCAGTTTAA GAACCTCCTA 
TATCTATACA ACTCCGAGCC AAGAATTGAG AGGAGCATGG ATCCTTTGAT TTGGTGGAAA 
ACAAACATCA AATACAGCCC CCTGTATAGG ATTGTTCGGC AGTTTCTTTC CGCTCCGGCA 
GCGAGTGCGT CCAACGAGGC ATTGTTTGGC GAATCTGCAC ATTTGTATAG TGATATGCAG 
GGGCATTTGA GTCCCGAAAG TGTATCCAAA ATATTGTTCA TCAAGTCCAA CTCTAAGGTC 
TCCGACAACA GAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015036922.1
CDS	89..1906
Translation	MSKKSNVWQFFYKANDTVATCCLCNRNYSRKGRGTTCLRNHLKSKHPVEFQTLAEDIRCLVKREVTYRLPQHPTSQLELKLHIAPDPLEMDNEDQLFARCDEHLAQMFLNHSFDLIEGPGFINFVKVLHSGYVIKARGYYENILCRDIHNRLHVQVKQQVDLLGAISLSTSLWWANNDGLLSLSCFGVTSDFKAQRLTLKCEALSYEDTDRVAYCIQGLISGLDMGLSREKVHCIIRDEATALAGALPDCSVSRLQMCVRFALQENQQLQNLYSKCKQIVEHFASSTMAQEQLKFIQEIRLKQEPCSIIAESPLRWNSIFDIMGRVLKMKDALSLYAEENSLVQIYPDEWLDIDLCKKVMQPCEEIIKMWSDSSTTASSVIPLVAALRDSLRTDVHNFVSSVTICSFARKLLEELEMKFSHITTDIKYLVATYLDPRYKQAFFNDREEQLVTNEVLQQLTSIQGNDEGQPSLQKVKISSSQIKNESKIDTILDNILASGSAHNQTATTSFSSQSQFKNLLYLYNSEPRIERSMDPLIWWKTNIKYSPLYRIVRQFLSAPAASASNEALFGESAHLYSDMQGHLSPESVSKILFIKSNSKVSDNRV

Target ORF information:

RefSeq Version	XM_015181436.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA12516), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015181436.1

ORF Insert Sequence:

ATGAGCAAGA AAAGCAATGT CTGGCAGTTT TTCTACAAGG CCAACGATAC GGTGGCCACA 
TGCTGTCTAT GCAACAGAAA TTACTCGAGA AAGGGCAGAG GCACCACCTG CCTGAGGAAC 
CATTTGAAAA GCAAGCACCC TGTGGAGTTT CAAACACTCG CCGAAGATAT CAGGTGCTTA 
GTGAAAAGAG AAGTAACTTA CAGGTTACCA CAGCATCCTA CATCGCAATT GGAACTGAAA 
CTGCATATCG CTCCTGATCC CTTGGAAATG GACAATGAAG ATCAGTTGTT TGCGCGCTGT 
GACGAACATT TGGCGCAAAT GTTTCTCAAT CACTCTTTCG ACTTGATAGA AGGCCCCGGA 
TTCATAAACT TTGTGAAAGT TTTGCACTCG GGATATGTGA TCAAAGCGAG GGGCTACTAT 
GAGAATATAT TGTGCCGCGA TATCCACAAT AGACTTCACG TTCAGGTGAA GCAGCAGGTG 
GATCTGCTAG GTGCTATATC GCTGAGCACC AGTCTGTGGT GGGCCAATAA CGATGGCCTG 
CTTAGCCTTT CGTGTTTTGG TGTCACGAGT GATTTCAAGG CTCAGCGACT TACGCTCAAA 
TGCGAGGCCC TTAGCTATGA GGACACCGAC AGAGTGGCCT ATTGTATTCA GGGTTTGATC 
TCAGGTTTGG ATATGGGGCT TTCTAGAGAG AAGGTTCATT GCATTATCCG AGATGAGGCA 
ACTGCTCTGG CCGGAGCACT TCCCGACTGC TCTGTCTCAC GCCTGCAAAT GTGTGTGAGA 
TTCGCCTTGC AAGAGAACCA ACAGCTCCAG AATCTCTACT CGAAATGCAA GCAAATTGTT 
GAGCATTTTG CGTCCTCCAC GATGGCCCAG GAGCAGCTGA AATTCATTCA AGAGATTCGC 
CTTAAACAAG AGCCGTGCTC AATCATCGCC GAAAGTCCCT TGAGATGGAA CTCCATTTTC 
GACATTATGG GCCGTGTGCT CAAGATGAAG GACGCTCTCT CGCTGTACGC CGAGGAAAAT 
AGTCTGGTCC AAATCTATCC AGATGAATGG CTGGACATTG ATTTGTGCAA GAAAGTAATG 
CAGCCTTGCG AGGAGATCAT CAAAATGTGG AGCGATTCTT CAACGACCGC CTCTTCGGTG 
ATTCCGCTTG TGGCTGCCTT GAGGGATTCT CTTCGAACAG ATGTCCACAA TTTTGTGTCC 
TCAGTGACCA TTTGCAGCTT TGCCCGTAAA CTGCTAGAAG AACTGGAAAT GAAGTTCTCT 
CATATTACCA CAGATATAAA ATATCTGGTG GCCACATATT TGGATCCGCG CTACAAGCAA 
GCCTTCTTCA ACGATCGCGA AGAGCAACTG GTCACCAACG AAGTGCTGCA GCAGCTAACC 
AGCATCCAAG GAAACGACGA GGGTCAGCCT TCTTTGCAAA AAGTTAAAAT ATCTTCTTCG 
CAAATTAAAA ATGAATCAAA GATTGATACC ATTTTGGATA ATATACTGGC CTCTGGCTCT 
GCCCATAATC AGACAGCCAC CACATCCTTT AGCTCTCAGT CGCAGTTTAA GAACCTCCTA 
TATCTATACA ACTCCGAGCC AAGAATTGAG AGGAGCATGG ATCCTTTGAT TTGGTGGAAA 
ACAAACATCA AATACAGCCC CCTGTATAGG ATTGTTCGGC AGTTTCTTTC CGCTCCGGCA 
GCGAGTGCGT CCAACGAGGC ATTGTTTGGC GAATCTGCAC ATTTGTATAG TGATATGCAG 
GGGCATTTGA GTCCCGAAAG TGTATCCAAA ATATTGTTCA TCAAGTCCAA CTCTAAGGTC 
TCCGACAACA GAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA12516 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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