Dpse\GA20145 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20145
Entrez Gene ID	4813946
Full Name	GA20145 gene product from transcript GA20145-RA
Synonyms	GA20145,dpse_GLEANR_12029
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354070.2	XP_001354106.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20145 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20145 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe08881	XM_001354070.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20145), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20145 ( XM_001354070.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354106.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20145), mRNA.

Price & Availability↑

CloneID	ODe08881
Clone ID Related Accession (Same CDS sequence)	XM_001354070.2
Accession Version	XM_001354070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_001354070.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGGCG TCCTCAAATG GTTGGAGCGC ATAGAGTTCC CAAGTACCAA AGATGGCGAG 
ACCAGCGTAC ACAGCGTTTG CTACGCCCCG GATGGCAGCC AATTAGTGGT TGCCGCCGGC 
GATCGTTTGC TCATCTATAA TCCCAGCGAT GGATCACTCC TGAATACGCT GAAGGCGCAC 
AAAGACACGG TGAACTGTGT GGCGTATTCA CGCGATGGCA AACGCTTTGC CAGCGGAGCC 
TCCGACAAAA TGGTCATTGT ATGGTCGCCG CAACTGGAAG GGCTACTTAA GTACTCGCAC 
GGCGATTCCA TTCAATGCAT GAGCTTCAAT CCCGTCTCGC ACCATTTGGC CTCCTGTTCG 
ATGTCCGATT TTGCGTTCTG GTCGGCGGAT CAGAAGGCTG TGCAAAAGTA CAAAATATCA 
GCTCGTGTAA ATGGCTGCTC TTGGACAAAC GATGGCCAGT ACCTCATCAT GGGCTTAGCC 
AATGGCACAA TCTCCATACG AAACAAGCTG GGCGAGGAGA AGGGCCGCAT CGATCGGCCG 
GGTGGGCCGA ACAGCAACGT TTTCAGTGTA CAATGTTGCC CAGCGGCCGG GCCAGGTAGT 
GTGGACACTA TTGCCGTGGT AGACTGGAGC CAAACACTCT CATTCCATAC GCTCAGCGGT 
CAAATGATAG GCAAGGAGAG AACCCTGGGC TTTGATCCCC TTTGTCTACA ATACTTTCCA 
AACGGAGAGT TCTGTTTGGT CGGCGGTTGC ACGGGCGGCC TTCATTTCTT CACACGCGAG 
GGGATACGAT TGGGAACTTT GGGCGACAGT TTCGACACGT GGATATGGTC CGTGGCGCTG 
CATCCGAACG GGCAGTCCTA CACCATTGGC TGCCAGGATG GAACGCTGGC CTGTTTCAAC 
ATAGCCTCGA GTACAGTGCA CGCCCTCTAC AGAGAACGAT ACGCCTTTCG AGAGAACATG 
TGCGATGTGA TCATTCAGCA TCTGATCTCT GGCCAAAAGG TGCGCATCAA GTGCCGGGAT 
CTCGTCCAGA AAATAGCCAT CTATCGCAAT CGGCTGGCAG TGCAGCTGCC AGAGCGAGTG 
GTTCTGTACG AGCTGAGCTC TGGTGAGGAT CAACCAATGC ACTACAAAGT CAGGGAGAAG 
ATTCAACAGA AGTTTGACTG CAGCCTGCTG GTGGTCTGCG GTCGCCACAT TGTCCTGTGC 
CAGGAGAAGC GCCTGCAGTG CCTGGACTTC ATTGGCATCC TGCAACGAGA ATGGATAATG 
GATTCCTTCA TACGATATAT CAAGGTGACT GGCGGCCCAG TCGGCCGTGA AGGCCTGATG 
GTGGGCTTGA AGAATGGTCA AGTATTTCGT ATCTTTCTCA ACAACTCGCT GCCCCTGCTG 
ATCACGACAG CCGTCTCAGC GGTCCGTTGC TTGGACATCA ATGCCAAACG TAGCAAAATC 
GCTGTGGTGG ATGATGTTGG CCGTCTGGTG GTGCGGGATA TTGTCAACGA CACCATACTC 
TATCAGGACA CTGGTGTGAA CAGCGTCACC TGGAACACCC ATCTCGATAC CATGCTCTGC 
TACACTCATA CCACGGGTGG CCTAAGTGTG AGAGTCGGAA ATTTGCCGCC TCGAGCCCCG 
CAGAATATGC ACGGCGTGGT GGTGGGTCTG TGTGGGGCCA CTGCTTTCTG TCTGCGCGGA 
AATATAATGC ACAACACTCC CCTGGCCTTG GGCTCCACCA TGTGGCAATT CATTGAGGCA 
GGCCTGTTTG AGGAAGCCTA CCAGGTGGCC TGCTTGGGAG TGACCACCGG CGACTGGGAG 
GGACTGGCCC AATCGGCCCT GGAAGCCCTG CATATCAATA TAGCCCGCGA TGCGTATGTC 
AAGGTGAGGA ATCTGCCGTG GCTCAAGCTG ATCAGCGAAC TGCGGGACCA ACAGCAACGT 
TCGGCAGTGC CCAAAGAGGT GCTTTTGGGC GAAAACTGTG CATTTGCTGG CAAATTCAAG 
GAAGCCGCTC GCTTGTTTCT CAAATGTGGA CAGACGCCAC GGGCTCTCGA GATGTACACC 
GATTTGCGCA TGTTCGATCT GGCCCAGGAG TATCTTAGAG ATGGCACTGA GCAGGAAAAG 
AAGGAACTTG TGCGCAAGCG AGCGGAATGG GCCTATTCCG TGAAGGAGCC GCGAGCTGCT 
GCCGAGCTCC TGCTTTCGGC TGGCGAGAAT CAAAAGGCCA TCGATATTGT GGCCGAACAG 
GGATGGGCGG ATGTTCTCTA CGATATCGGA CGGCGCCTCA GCCTCAGCGA TCGCGATGCC 
TTGGAATCGG TGGCGCAAAA TTTGAAGACT CTAAAAGCCC TCCCCCTGGC TGCTGAAATA 
TTCAAGAAGC TCGGGGACGA GGCTCAGGTC GTGCAGTTGC ACGTAGAGGT TCGTGACTGG 
CCGGAGGCGT TTCGTCTGGC GGAATCGTTG TCCGAGCTGC TGCCCACGGT GCACTACCAG 
CATGGCCAAT GGCTCGCCGA AACGGATCAG TTTATAGAGG CACATCAGGC ATATCTGAAG 
GCAGGACGGA CCAAGGAAGC CAATCGCTTG CTGAAGCAGT TGAGCATCAC GGCCATCTTG 
GAGGAGCGTT ATCTAGATGC GAGCTACTTT TACTGGCTAC TGGCCAAGCA ATCTCTGGAC 
ATTTACCATG CAAAGGATGA ACAGAATCCA ATCGATCATA ATCTGGCCGA ATACAGAAAT 
CATTTGCGCA TTGCCAAGGT GTACTATGCC TATGCCGTGA TCTACAGCTA CATGAAGGAA 
CCCTTCACCA CTCACTCGCC CGTCAGTCTG TTCAACGTCA GCCGCTTCAT ACTTAATGAG 
GTGGAGCACA AGGGCGTGCC CAAGGGTGTG AGCATGTTCT CTGTTCTGTT TACCTTGGCT 
AAGCAGGCGA AGTTCCTGCA GGCCAACAAA TTGTGTTTGC TGATCAACAA GCGACTACAG 
TCACTCAAAC CACCGGCCGG AGTACAAGAG CAGATTGATC TAAATTTCCT AAACAGCAAG 
GCATGCAAAA GTGGCTTCAA TGATCCCGAG GAGCTGCTGC CTCTGTGCTA CAAGTGTTCT 
AACTACAGTC AACATTTGAA CAGCAATTGC TGCCCGACCT GCAGGCAGGA CTACATATTC 
TCGTTTGTTT CATTTGAAAT CCTACCCCTA GTACAATTCT ATCCCGAAGT GGATATATCG 
GATGCCGAGG CCGAGCGTTT GCTACTGGCC CCGGCCAAGC ATGTGGAGGA TACAGATCCC 
TTCAACGAAG ATGTGGCCAG TGCTTTGCCC TTGAGCTTGG ATCGGAATGG TCTGCGTGCC 
ATAGATCCAA ATCATGTGCT GATACTCAAG CGACAGGGCA AGAACGTACG AAATGTGTAC 
TATCGCAATA TTTTGCCCGA TCTACAAGTC ACCTATTGCC CACAGTGTCT GCTGCTCTTC 
TATGCCGAGG ACTTTGAGCT GCAGGTGCTG CAAAAGGGAT ACTGTCCGTT TTGTCACACA 
TCCTCGGATA AGTTGATGGA AGACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354106.1
CDS	98..3607
Translation	MRGVLKWLERIEFPSTKDGETSVHSVCYAPDGSQLVVAAGDRLLIYNPSDGSLLNTLKAHKDTVNCVAYSRDGKRFASGASDKMVIVWSPQLEGLLKYSHGDSIQCMSFNPVSHHLASCSMSDFAFWSADQKAVQKYKISARVNGCSWTNDGQYLIMGLANGTISIRNKLGEEKGRIDRPGGPNSNVFSVQCCPAAGPGSVDTIAVVDWSQTLSFHTLSGQMIGKERTLGFDPLCLQYFPNGEFCLVGGCTGGLHFFTREGIRLGTLGDSFDTWIWSVALHPNGQSYTIGCQDGTLACFNIASSTVHALYRERYAFRENMCDVIIQHLISGQKVRIKCRDLVQKIAIYRNRLAVQLPERVVLYELSSGEDQPMHYKVREKIQQKFDCSLLVVCGRHIVLCQEKRLQCLDFIGILQREWIMDSFIRYIKVTGGPVGREGLMVGLKNGQVFRIFLNNSLPLLITTAVSAVRCLDINAKRSKIAVVDDVGRLVVRDIVNDTILYQDTGVNSVTWNTHLDTMLCYTHTTGGLSVRVGNLPPRAPQNMHGVVVGLCGATAFCLRGNIMHNTPLALGSTMWQFIEAGLFEEAYQVACLGVTTGDWEGLAQSALEALHINIARDAYVKVRNLPWLKLISELRDQQQRSAVPKEVLLGENCAFAGKFKEAARLFLKCGQTPRALEMYTDLRMFDLAQEYLRDGTEQEKKELVRKRAEWAYSVKEPRAAAELLLSAGENQKAIDIVAEQGWADVLYDIGRRLSLSDRDALESVAQNLKTLKALPLAAEIFKKLGDEAQVVQLHVEVRDWPEAFRLAESLSELLPTVHYQHGQWLAETDQFIEAHQAYLKAGRTKEANRLLKQLSITAILEERYLDASYFYWLLAKQSLDIYHAKDEQNPIDHNLAEYRNHLRIAKVYYAYAVIYSYMKEPFTTHSPVSLFNVSRFILNEVEHKGVPKGVSMFSVLFTLAKQAKFLQANKLCLLINKRLQSLKPPAGVQEQIDLNFLNSKACKSGFNDPEELLPLCYKCSNYSQHLNSNCCPTCRQDYIFSFVSFEILPLVQFYPEVDISDAEAERLLLAPAKHVEDTDPFNEDVASALPLSLDRNGLRAIDPNHVLILKRQGKNVRNVYYRNILPDLQVTYCPQCLLLFYAEDFELQVLQKGYCPFCHTSSDKLMEDF

Target ORF information:

RefSeq Version	XM_001354070.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20145), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354070.2

ORF Insert Sequence:

ATGCGTGGCG TCCTCAAATG GTTGGAGCGC ATAGAGTTCC CAAGTACCAA AGATGGCGAG 
ACCAGCGTAC ACAGCGTTTG CTACGCCCCG GATGGCAGCC AATTAGTGGT TGCCGCCGGC 
GATCGTTTGC TCATCTATAA TCCCAGCGAT GGATCACTCC TGAATACGCT GAAGGCGCAC 
AAAGACACGG TGAACTGTGT GGCGTATTCA CGCGATGGCA AACGCTTTGC CAGCGGAGCC 
TCCGACAAAA TGGTCATTGT ATGGTCGCCG CAACTGGAAG GGCTACTTAA GTACTCGCAC 
GGCGATTCCA TTCAATGCAT GAGCTTCAAT CCCGTCTCGC ACCATTTGGC CTCCTGTTCG 
ATGTCCGATT TTGCGTTCTG GTCGGCGGAT CAGAAGGCTG TGCAAAAGTA CAAAATATCA 
GCTCGTGTAA ATGGCTGCTC TTGGACAAAC GATGGCCAGT ACCTCATCAT GGGCTTAGCC 
AATGGCACAA TCTCCATACG AAACAAGCTG GGCGAGGAGA AGGGCCGCAT CGATCGGCCG 
GGTGGGCCGA ACAGCAACGT TTTCAGTGTA CAATGTTGCC CAGCGGCCGG GCCAGGTAGT 
GTGGACACTA TTGCCGTGGT AGACTGGAGC CAAACACTCT CATTCCATAC GCTCAGCGGT 
CAAATGATAG GCAAGGAGAG AACCCTGGGC TTTGATCCCC TTTGTCTACA ATACTTTCCA 
AACGGAGAGT TCTGTTTGGT CGGCGGTTGC ACGGGCGGCC TTCATTTCTT CACACGCGAG 
GGGATACGAT TGGGAACTTT GGGCGACAGT TTCGACACGT GGATATGGTC CGTGGCGCTG 
CATCCGAACG GGCAGTCCTA CACCATTGGC TGCCAGGATG GAACGCTGGC CTGTTTCAAC 
ATAGCCTCGA GTACAGTGCA CGCCCTCTAC AGAGAACGAT ACGCCTTTCG AGAGAACATG 
TGCGATGTGA TCATTCAGCA TCTGATCTCT GGCCAAAAGG TGCGCATCAA GTGCCGGGAT 
CTCGTCCAGA AAATAGCCAT CTATCGCAAT CGGCTGGCAG TGCAGCTGCC AGAGCGAGTG 
GTTCTGTACG AGCTGAGCTC TGGTGAGGAT CAACCAATGC ACTACAAAGT CAGGGAGAAG 
ATTCAACAGA AGTTTGACTG CAGCCTGCTG GTGGTCTGCG GTCGCCACAT TGTCCTGTGC 
CAGGAGAAGC GCCTGCAGTG CCTGGACTTC ATTGGCATCC TGCAACGAGA ATGGATAATG 
GATTCCTTCA TACGATATAT CAAGGTGACT GGCGGCCCAG TCGGCCGTGA AGGCCTGATG 
GTGGGCTTGA AGAATGGTCA AGTATTTCGT ATCTTTCTCA ACAACTCGCT GCCCCTGCTG 
ATCACGACAG CCGTCTCAGC GGTCCGTTGC TTGGACATCA ATGCCAAACG TAGCAAAATC 
GCTGTGGTGG ATGATGTTGG CCGTCTGGTG GTGCGGGATA TTGTCAACGA CACCATACTC 
TATCAGGACA CTGGTGTGAA CAGCGTCACC TGGAACACCC ATCTCGATAC CATGCTCTGC 
TACACTCATA CCACGGGTGG CCTAAGTGTG AGAGTCGGAA ATTTGCCGCC TCGAGCCCCG 
CAGAATATGC ACGGCGTGGT GGTGGGTCTG TGTGGGGCCA CTGCTTTCTG TCTGCGCGGA 
AATATAATGC ACAACACTCC CCTGGCCTTG GGCTCCACCA TGTGGCAATT CATTGAGGCA 
GGCCTGTTTG AGGAAGCCTA CCAGGTGGCC TGCTTGGGAG TGACCACCGG CGACTGGGAG 
GGACTGGCCC AATCGGCCCT GGAAGCCCTG CATATCAATA TAGCCCGCGA TGCGTATGTC 
AAGGTGAGGA ATCTGCCGTG GCTCAAGCTG ATCAGCGAAC TGCGGGACCA ACAGCAACGT 
TCGGCAGTGC CCAAAGAGGT GCTTTTGGGC GAAAACTGTG CATTTGCTGG CAAATTCAAG 
GAAGCCGCTC GCTTGTTTCT CAAATGTGGA CAGACGCCAC GGGCTCTCGA GATGTACACC 
GATTTGCGCA TGTTCGATCT GGCCCAGGAG TATCTTAGAG ATGGCACTGA GCAGGAAAAG 
AAGGAACTTG TGCGCAAGCG AGCGGAATGG GCCTATTCCG TGAAGGAGCC GCGAGCTGCT 
GCCGAGCTCC TGCTTTCGGC TGGCGAGAAT CAAAAGGCCA TCGATATTGT GGCCGAACAG 
GGATGGGCGG ATGTTCTCTA CGATATCGGA CGGCGCCTCA GCCTCAGCGA TCGCGATGCC 
TTGGAATCGG TGGCGCAAAA TTTGAAGACT CTAAAAGCCC TCCCCCTGGC TGCTGAAATA 
TTCAAGAAGC TCGGGGACGA GGCTCAGGTC GTGCAGTTGC ACGTAGAGGT TCGTGACTGG 
CCGGAGGCGT TTCGTCTGGC GGAATCGTTG TCCGAGCTGC TGCCCACGGT GCACTACCAG 
CATGGCCAAT GGCTCGCCGA AACGGATCAG TTTATAGAGG CACATCAGGC ATATCTGAAG 
GCAGGACGGA CCAAGGAAGC CAATCGCTTG CTGAAGCAGT TGAGCATCAC GGCCATCTTG 
GAGGAGCGTT ATCTAGATGC GAGCTACTTT TACTGGCTAC TGGCCAAGCA ATCTCTGGAC 
ATTTACCATG CAAAGGATGA ACAGAATCCA ATCGATCATA ATCTGGCCGA ATACAGAAAT 
CATTTGCGCA TTGCCAAGGT GTACTATGCC TATGCCGTGA TCTACAGCTA CATGAAGGAA 
CCCTTCACCA CTCACTCGCC CGTCAGTCTG TTCAACGTCA GCCGCTTCAT ACTTAATGAG 
GTGGAGCACA AGGGCGTGCC CAAGGGTGTG AGCATGTTCT CTGTTCTGTT TACCTTGGCT 
AAGCAGGCGA AGTTCCTGCA GGCCAACAAA TTGTGTTTGC TGATCAACAA GCGACTACAG 
TCACTCAAAC CACCGGCCGG AGTACAAGAG CAGATTGATC TAAATTTCCT AAACAGCAAG 
GCATGCAAAA GTGGCTTCAA TGATCCCGAG GAGCTGCTGC CTCTGTGCTA CAAGTGTTCT 
AACTACAGTC AACATTTGAA CAGCAATTGC TGCCCGACCT GCAGGCAGGA CTACATATTC 
TCGTTTGTTT CATTTGAAAT CCTACCCCTA GTACAATTCT ATCCCGAAGT GGATATATCG 
GATGCCGAGG CCGAGCGTTT GCTACTGGCC CCGGCCAAGC ATGTGGAGGA TACAGATCCC 
TTCAACGAAG ATGTGGCCAG TGCTTTGCCC TTGAGCTTGG ATCGGAATGG TCTGCGTGCC 
ATAGATCCAA ATCATGTGCT GATACTCAAG CGACAGGGCA AGAACGTACG AAATGTGTAC 
TATCGCAATA TTTTGCCCGA TCTACAAGTC ACCTATTGCC CACAGTGTCT GCTGCTCTTC 
TATGCCGAGG ACTTTGAGCT GCAGGTGCTG CAAAAGGGAT ACTGTCCGTT TTGTCACACA 
TCCTCGGATA AGTTGATGGA AGACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20145 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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