Dpse\GA20146 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20146
Entrez Gene ID	4812815
Full Name	GA20146 gene product from transcript GA20146-RB
Synonyms	GA20146,dpse_GLEANR_13038
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001353493.3	XP_001353529.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20146 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20146 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe25746	XM_001353493.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20146), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20146 ( XM_001353493.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001353529.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20146), mRNA.

Price & Availability↑

CloneID	ODe25746
Clone ID Related Accession (Same CDS sequence)	XM_001353493.3
Accession Version	XM_001353493.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_001353493.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGAA CAAATGCAAA ATGCTCGTTG GGGGCGGGAG GTGGCTTTGG TAGTCATATA 
CCCAGCATTG AGGAGAAGAA TAGGCAAATC CAACAGGAGA CCATGATGGA GAAACTGAGA 
GCAAAGTACC GGAACAAGCC GAAAACCTAT GAAGAGATCA AGAAGTCGGT GAGAATGTAT 
TTCATTGAGA AGCACTATGC ACTGGATCCG CTGTGCAAGG CAACACTGCA GTCGGCATTG 
GACAAGCTTC AGCATTACAT CAAGGTTACC TCGAGGCATG GTCTCGTGGA GCGATTGGAA 
AGTCTGTCCC GGCAATTGGG TCTCAAGTTC ATGGAGGATC AGCAGCTTCT GTTCATTTCC 
ACTGATATGT TCTATGTGGA AATTCTTCTC GACGCCAGTG GTAATCTGTC GGACGTCAAG 
GTGCATCACG AATGCAAAAT AGAGCAGCAA TCGAGTGAAT TGGTGAAATG CCTAAAGTCC 
GGAGACTTTG CCGACTTCAC CGTCCAGCTG GAGGGTCTGT CCTCAATTTA CCAACTCAAT 
GCCGAGCCAA AAGTGAAGAA GAAGGCGTTT GTGGCCTTGC AGGCCATGGA AACGGACATC 
TACAGTCTCT ATAGTCATCA GATGCAAAAC CGCACCGGTG ACAGCTACGA TATCATGAAC 
AGCTCTGCCG TTGGTCTGGT GCTGCAGCGG CGCGGCGGTC ATCCCATGAA GCTGACCTAC 
TTCTGCCCGC CCATCCACCT CGCGGAGGGC GAATCGAAGT TGGCAAGCAG CGAGTTTAGC 
ATTGATCAGG TGATGCACAG CAGCCACGGC CTAAGTGCCA CCGTTAACCT GGAGGGTTCC 
TCGGCCAACA AACTTCAGAT TATGCCCATT TTAAGCTTCA GTCGCGATCC TCAAACAGGA 
CTGGAGCTGC CCACGTATGC GCAGCTCAAT CAAAACAACT CTATGCTGAT GCCGGCCACA 
TATGTGTTGC GTCTAAACAA GCCGATGCCC GTGTGTTACG AAACCCTCAA GAGTCTAGGT 
CTGCCAGGCG TGGAAGGCAT GGCTGCAGCA CCCTCGCCGC CAGTCGCCAC CGTGCTCAAT 
TTGATTGTGC AAACCGCCTC TAAGCAGGCG ATAAAGAACA CCCAGCGAGG CCTCTATGTC 
AATCTGCCCA AGGAGACGCA CTGCTATTTC TTTACGGACA ATCGGAAGCT GCAAGGTACA 
CTGATATCCT CCCTTCCCTT CACAGAGCCC GCCCAGGTAC CCAAGATAAT GACGTTTCTG 
AAGAAGCAGG CCCTCTTCTA CACGCTGCTC GCTAGCTGTG TGCGAGAACA ACAGAAGCAG 
TACAATGACA TGGATAGCAC TGTGATATTG GAGGTGACGG CCGTTTCCTT CAACCAGATC 
ACTGTGGAGC TGCAGCACCC CTATGAGGAG TCGCTGGCCA CAGTGGATTT TCTTCTGGAG 
GATGGCCAGG CCACATGCAG CATCTATTGT TTGAACAACG AGTACGAGGT GCTCTCCCAA 
AAGCTGACTC GGACTGTGCG GAAGGTCCTC TCCATCCCGA TGGTCATCTA CAAGCTGCTC 
AAGTGTTGGG ATGAGGAGCA TCAGTTCAAG CTGCATGGTG TGATTGGTTC TGGAGCAGGC 
GCTGGTGCGG GATCCGGAGC AGGAGCTGGA ACAGGCAACG GAGGCGGAGT CGGGACTGGA 
CTTGCTCATG CCCATAATTT TAATCAATTT GCCATGGAAG CGGGACCTTC GCTGGATGGC 
AGCCTGCCCA GCGGAGGCTT CACCAGCATC AACAGCCTCA AAATGGAAGC CAAGTCGCGC 
TCCCTGGCAG ATGCTTTTGT GGCGTCCACC TCGGCAGCGG CAGCCATTGC CAGCCTAATA 
AATCTCAAGC GTGAAACGGA TCCTCAATCA GCCCTTGGCC CTGTGTCCTC GGGCAACTCC 
AGCAGCTCCG GCAGCTCATC CACCGTGCTG GCGGCGGCCA AGGCGACTGA TCATGAGATT 
GCCGACAAGT ATAAGAACAT TTGGAAGGAT AAAACACCGA ATCTGAAGCA CTGCGTCAGC 
ATAACTCCCA TACCAGGTGA CGGTAAGACA GGAGGAGGAG GCGCAGGAGG GGGTCCCGTG 
GGAAGCAGTG TTGGCGGAGT AGTTCCAACA GTGGATGTTC AGCGTACAGG TGGCATAGAG 
ATAATACCAT TAAACGCACA GAGCCAGACT AGTGGAACTC GGGACGGGGG AAGTGCGACC 
ACCACAACGG CACCCACTAC CATTACCATA ACTCCGATAA CTGGCAAGGA CCCCGCCAAG 
GAATCGTCCA AGAAGAGCAG CTCTTCAGCT CCTGCCGGGG TAAGTATAGC CACGAAGCGC 
CCTCATGAAA GCAGCACCAG CGCTTCGTCA TCGTCAGCCG GCAGCGGAGG CAGCAGCTCC 
TCCACATCTT CCACCGGCAG CAGCAGTTCA TGCTCCACAG ATACACAGAA GGAGAAGAAG 
CGCAAGAAGA AACGCGATGA TTCTCCCATG GGACCCCCGG AGAAGATATA CTCCCGACAG 
AACTCTCCAG CTGCGGGTGG AGATGCCTCG GCTACCGGGG GCGTGGTAAG AAAGTTTTCC 
TCTCCCTCGT CCTCACCCAA AGCTGGTGGA TCCGGGCAGG GCATACTGTC TGGCGTGCCC 
CCAGCACGTC CCAGTCCCAA GCATTCGCCC GTCTACAGCA GCCCCAAGCA CAACACCGCC 
TCGAACAGTC CCAAATCGCC GTTTGGGACG CACTCACCCA AGCACGGCTC CTCGGGGAAG 
CCGAGCATGT CCACTCTGAA GAGTGCGGCC ACAGCGGGAA CTAGCCTCAG TCCCAAGGGT 
GACAAATCAA CGTCATCCGT AGGCCCTCCT CCTTCAGCGT CTGTAGGATC ATCGACAGGA 
GCTAGCGCCA ATCCAAGTCT GGTGCGTTCC TTTGCAAGCG TTGGAGCACC GCCACCACCA 
CCGCCTGTTC CTCCCTTGAG CAGTGCCATC AGTAGCAGCA GCAGCAGCAG CAGCAGCTGC 
AGCAGCAGCG GCAGCGCCGG CGGAAGCAGC AGTCAGCAAA GCGGCAGCTC GGGCATTAAA 
AAGGAGAAAT CTTCAGCCGC GGGACCCTCG TCGTCGTCGG GTGCTTCTTC TGGCCCCTCC 
GGTGGTAGCG GCGGCGTCGG TGGTGTTTCT CCAGCCAGTG TGGTGTCTGT CGCCAGTGTA 
GCTGCTGCTG TGGCGGCTCT GAAAACCTCC CAACAGCAGC AACAACAGCA GCAGCTCAAA 
TCATCTGTGG CCAGCTTGTC GCATCTGGCA GCTGGCGGCA GCTTGGGCAG CTATGCGGCA 
GCTGGAGGAG CAGCAGCGGT GGCTGCAGCG GCGGCAACGG TGGGAGCAGG AGCGGGAGCA 
GTGACAGGTG CTTCGGCCTT GGAACTGAGT GCTCTGCGGA AGGGCCTGGC GGGAGGCGCG 
GTAAGTCCGT ATTCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001353529.3
CDS	192..3632
Translation	MSGTNAKCSLGAGGGFGSHIPSIEEKNRQIQQETMMEKLRAKYRNKPKTYEEIKKSVRMYFIEKHYALDPLCKATLQSALDKLQHYIKVTSRHGLVERLESLSRQLGLKFMEDQQLLFISTDMFYVEILLDASGNLSDVKVHHECKIEQQSSELVKCLKSGDFADFTVQLEGLSSIYQLNAEPKVKKKAFVALQAMETDIYSLYSHQMQNRTGDSYDIMNSSAVGLVLQRRGGHPMKLTYFCPPIHLAEGESKLASSEFSIDQVMHSSHGLSATVNLEGSSANKLQIMPILSFSRDPQTGLELPTYAQLNQNNSMLMPATYVLRLNKPMPVCYETLKSLGLPGVEGMAAAPSPPVATVLNLIVQTASKQAIKNTQRGLYVNLPKETHCYFFTDNRKLQGTLISSLPFTEPAQVPKIMTFLKKQALFYTLLASCVREQQKQYNDMDSTVILEVTAVSFNQITVELQHPYEESLATVDFLLEDGQATCSIYCLNNEYEVLSQKLTRTVRKVLSIPMVIYKLLKCWDEEHQFKLHGVIGSGAGAGAGSGAGAGTGNGGGVGTGLAHAHNFNQFAMEAGPSLDGSLPSGGFTSINSLKMEAKSRSLADAFVASTSAAAAIASLINLKRETDPQSALGPVSSGNSSSSGSSSTVLAAAKATDHEIADKYKNIWKDKTPNLKHCVSITPIPGDGKTGGGGAGGGPVGSSVGGVVPTVDVQRTGGIEIIPLNAQSQTSGTRDGGSATTTTAPTTITITPITGKDPAKESSKKSSSSAPAGVSIATKRPHESSTSASSSSAGSGGSSSSTSSTGSSSSCSTDTQKEKKRKKKRDDSPMGPPEKIYSRQNSPAAGGDASATGGVVRKFSSPSSSPKAGGSGQGILSGVPPARPSPKHSPVYSSPKHNTASNSPKSPFGTHSPKHGSSGKPSMSTLKSAATAGTSLSPKGDKSTSSVGPPPSASVGSSTGASANPSLVRSFASVGAPPPPPPVPPLSSAISSSSSSSSSCSSSGSAGGSSSQQSGSSGIKKEKSSAAGPSSSSGASSGPSGGSGGVGGVSPASVVSVASVAAAVAALKTSQQQQQQQQLKSSVASLSHLAAGGSLGSYAAAGGAAAVAAAAATVGAGAGAVTGASALELSALRKGLAGGAVSPYSL

Target ORF information:

RefSeq Version	XM_001353493.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA20146), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001353493.3

ORF Insert Sequence:

ATGAGTGGAA CAAATGCAAA ATGCTCGTTG GGGGCGGGAG GTGGCTTTGG TAGTCATATA 
CCCAGCATTG AGGAGAAGAA TAGGCAAATC CAACAGGAGA CCATGATGGA GAAACTGAGA 
GCAAAGTACC GGAACAAGCC GAAAACCTAT GAAGAGATCA AGAAGTCGGT GAGAATGTAT 
TTCATTGAGA AGCACTATGC ACTGGATCCG CTGTGCAAGG CAACACTGCA GTCGGCATTG 
GACAAGCTTC AGCATTACAT CAAGGTTACC TCGAGGCATG GTCTCGTGGA GCGATTGGAA 
AGTCTGTCCC GGCAATTGGG TCTCAAGTTC ATGGAGGATC AGCAGCTTCT GTTCATTTCC 
ACTGATATGT TCTATGTGGA AATTCTTCTC GACGCCAGTG GTAATCTGTC GGACGTCAAG 
GTGCATCACG AATGCAAAAT AGAGCAGCAA TCGAGTGAAT TGGTGAAATG CCTAAAGTCC 
GGAGACTTTG CCGACTTCAC CGTCCAGCTG GAGGGTCTGT CCTCAATTTA CCAACTCAAT 
GCCGAGCCAA AAGTGAAGAA GAAGGCGTTT GTGGCCTTGC AGGCCATGGA AACGGACATC 
TACAGTCTCT ATAGTCATCA GATGCAAAAC CGCACCGGTG ACAGCTACGA TATCATGAAC 
AGCTCTGCCG TTGGTCTGGT GCTGCAGCGG CGCGGCGGTC ATCCCATGAA GCTGACCTAC 
TTCTGCCCGC CCATCCACCT CGCGGAGGGC GAATCGAAGT TGGCAAGCAG CGAGTTTAGC 
ATTGATCAGG TGATGCACAG CAGCCACGGC CTAAGTGCCA CCGTTAACCT GGAGGGTTCC 
TCGGCCAACA AACTTCAGAT TATGCCCATT TTAAGCTTCA GTCGCGATCC TCAAACAGGA 
CTGGAGCTGC CCACGTATGC GCAGCTCAAT CAAAACAACT CTATGCTGAT GCCGGCCACA 
TATGTGTTGC GTCTAAACAA GCCGATGCCC GTGTGTTACG AAACCCTCAA GAGTCTAGGT 
CTGCCAGGCG TGGAAGGCAT GGCTGCAGCA CCCTCGCCGC CAGTCGCCAC CGTGCTCAAT 
TTGATTGTGC AAACCGCCTC TAAGCAGGCG ATAAAGAACA CCCAGCGAGG CCTCTATGTC 
AATCTGCCCA AGGAGACGCA CTGCTATTTC TTTACGGACA ATCGGAAGCT GCAAGGTACA 
CTGATATCCT CCCTTCCCTT CACAGAGCCC GCCCAGGTAC CCAAGATAAT GACGTTTCTG 
AAGAAGCAGG CCCTCTTCTA CACGCTGCTC GCTAGCTGTG TGCGAGAACA ACAGAAGCAG 
TACAATGACA TGGATAGCAC TGTGATATTG GAGGTGACGG CCGTTTCCTT CAACCAGATC 
ACTGTGGAGC TGCAGCACCC CTATGAGGAG TCGCTGGCCA CAGTGGATTT TCTTCTGGAG 
GATGGCCAGG CCACATGCAG CATCTATTGT TTGAACAACG AGTACGAGGT GCTCTCCCAA 
AAGCTGACTC GGACTGTGCG GAAGGTCCTC TCCATCCCGA TGGTCATCTA CAAGCTGCTC 
AAGTGTTGGG ATGAGGAGCA TCAGTTCAAG CTGCATGGTG TGATTGGTTC TGGAGCAGGC 
GCTGGTGCGG GATCCGGAGC AGGAGCTGGA ACAGGCAACG GAGGCGGAGT CGGGACTGGA 
CTTGCTCATG CCCATAATTT TAATCAATTT GCCATGGAAG CGGGACCTTC GCTGGATGGC 
AGCCTGCCCA GCGGAGGCTT CACCAGCATC AACAGCCTCA AAATGGAAGC CAAGTCGCGC 
TCCCTGGCAG ATGCTTTTGT GGCGTCCACC TCGGCAGCGG CAGCCATTGC CAGCCTAATA 
AATCTCAAGC GTGAAACGGA TCCTCAATCA GCCCTTGGCC CTGTGTCCTC GGGCAACTCC 
AGCAGCTCCG GCAGCTCATC CACCGTGCTG GCGGCGGCCA AGGCGACTGA TCATGAGATT 
GCCGACAAGT ATAAGAACAT TTGGAAGGAT AAAACACCGA ATCTGAAGCA CTGCGTCAGC 
ATAACTCCCA TACCAGGTGA CGGTAAGACA GGAGGAGGAG GCGCAGGAGG GGGTCCCGTG 
GGAAGCAGTG TTGGCGGAGT AGTTCCAACA GTGGATGTTC AGCGTACAGG TGGCATAGAG 
ATAATACCAT TAAACGCACA GAGCCAGACT AGTGGAACTC GGGACGGGGG AAGTGCGACC 
ACCACAACGG CACCCACTAC CATTACCATA ACTCCGATAA CTGGCAAGGA CCCCGCCAAG 
GAATCGTCCA AGAAGAGCAG CTCTTCAGCT CCTGCCGGGG TAAGTATAGC CACGAAGCGC 
CCTCATGAAA GCAGCACCAG CGCTTCGTCA TCGTCAGCCG GCAGCGGAGG CAGCAGCTCC 
TCCACATCTT CCACCGGCAG CAGCAGTTCA TGCTCCACAG ATACACAGAA GGAGAAGAAG 
CGCAAGAAGA AACGCGATGA TTCTCCCATG GGACCCCCGG AGAAGATATA CTCCCGACAG 
AACTCTCCAG CTGCGGGTGG AGATGCCTCG GCTACCGGGG GCGTGGTAAG AAAGTTTTCC 
TCTCCCTCGT CCTCACCCAA AGCTGGTGGA TCCGGGCAGG GCATACTGTC TGGCGTGCCC 
CCAGCACGTC CCAGTCCCAA GCATTCGCCC GTCTACAGCA GCCCCAAGCA CAACACCGCC 
TCGAACAGTC CCAAATCGCC GTTTGGGACG CACTCACCCA AGCACGGCTC CTCGGGGAAG 
CCGAGCATGT CCACTCTGAA GAGTGCGGCC ACAGCGGGAA CTAGCCTCAG TCCCAAGGGT 
GACAAATCAA CGTCATCCGT AGGCCCTCCT CCTTCAGCGT CTGTAGGATC ATCGACAGGA 
GCTAGCGCCA ATCCAAGTCT GGTGCGTTCC TTTGCAAGCG TTGGAGCACC GCCACCACCA 
CCGCCTGTTC CTCCCTTGAG CAGTGCCATC AGTAGCAGCA GCAGCAGCAG CAGCAGCTGC 
AGCAGCAGCG GCAGCGCCGG CGGAAGCAGC AGTCAGCAAA GCGGCAGCTC GGGCATTAAA 
AAGGAGAAAT CTTCAGCCGC GGGACCCTCG TCGTCGTCGG GTGCTTCTTC TGGCCCCTCC 
GGTGGTAGCG GCGGCGTCGG TGGTGTTTCT CCAGCCAGTG TGGTGTCTGT CGCCAGTGTA 
GCTGCTGCTG TGGCGGCTCT GAAAACCTCC CAACAGCAGC AACAACAGCA GCAGCTCAAA 
TCATCTGTGG CCAGCTTGTC GCATCTGGCA GCTGGCGGCA GCTTGGGCAG CTATGCGGCA 
GCTGGAGGAG CAGCAGCGGT GGCTGCAGCG GCGGCAACGG TGGGAGCAGG AGCGGGAGCA 
GTGACAGGTG CTTCGGCCTT GGAACTGAGT GCTCTGCGGA AGGGCCTGGC GGGAGGCGCG 
GTAAGTCCGT ATTCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20146 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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