Dpse\GA18529 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA18529
Entrez Gene ID	4812399
Full Name	GA18529 gene product from transcript GA18529-RA
Synonyms	GA18529,dpse_GLEANR_8050
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001352529.3	XP_001352565.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA18529 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA18529 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe07664	XM_001352529.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18529), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA18529 ( XM_001352529.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001352565.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18529), mRNA.

Price & Availability↑

CloneID	ODe07664
Clone ID Related Accession (Same CDS sequence)	XM_001352529.3
Accession Version	XM_001352529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589954). On Dec 22, 2015 this sequence version replaced XM_001352529.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACAG CGCAGGCCCC CCCGCCGGCT GAGGAGAGGA GTGAAGAGAA GGTGTCTGAG 
ATGGATACTT CTCAGATAAA CAACCATCTG GAGGGAGAAA CAACAAATGA CAGTGGAGAC 
GTGGAGCAGC AGCATCTGGA ATGCGATAAA ATAGAGCAGA CTGCTGAAAG AAAGCCAACA 
AAGGCGAAAG CGGATGACGA GAAACCAGTT GCGGGAGACG AACTTCTCGT TGAGCATTTG 
AAGGCAGCGG TGCTGCAACA AATGCCTGTG CCTGTGCAGG ATGAAGACCA ATCGGGTGTC 
AGCATGCGCT TCAGAGACCT GCAAGTCCAA GACCAGGAAC AGAATCTCAA AAAGCTGCCA 
CAGAACGAGA TCCTATCCCA TGTGCACTGT CTGGCCCAGT TGGAGGAGCA GCGTCGCAGC 
TATGAGCAGC AGATGGAGCA GCTGCGAACT TCCAATGCCC AGAAGGACAA CATGATGACG 
CTCCTGCAGC GCGAGAATGC CATCTTGGAC AAGGAGAAGC AGGCCTTTCG TAAGGAAATG 
GATATGGCCA ACAAGGAGAA GGAGACCACA GTCATTAAGT TTGCCATGAA GGAGAAACTA 
CTCATCGATG CCAAAAAGGA GAAGGAGGCG GTGGATAAGC AGCTGGCGGA TACCAAGAAA 
GAGGTGAAGA ATGTTTCTAC CCGCTTCATG GCCATCCACG AGGAAAAGTC CAGAATGACG 
TACATAATAG ATGAAAAGTG CAACGAAGTT AGGAAATATC AGCGCGAATG CGAAAAGTAC 
AAAACGGAGA TGAGCCACCT AGAGTCCAAG CTAAAGTATC ACATGAACAA GCTGTCCATC 
GAAACGGAGG CCAAGGCGGC CCTGGAGCGC AAGCTGGAAG AGGAGCGAAA TGCACCGAAT 
AAGCTGGAGG AGAAGGCCAA TGAGAAGCTA AGGATGGAAT TTGAAGCAAA TACAATACTC 
TTGAAGCACG AGATCACAAG CAAGACTGAG GCCCTCGAAA AAGTCACCAA AGAGCAGCAA 
AAGCAAAGTG AAGCCAACAA AGAGCTGCAG CAGCAGCTAC AAGGGCTCGC CTTGGAGCAC 
ACTCAACTCA CGGAAGAGTT TAATCGACTA CGAGAGCAGC ACCATGGAGT CGAGGCGGCC 
TACAGCGATG AGCTGCTCAA TTCGGCCAAG CTACGGGGAC AACTCGAGGA ACTACAACTG 
CTGCGCACCC AAAACACCAT AAATGAAGAA AAGCTGTCCG AACAGCAGGC GAGAGTGCAG 
CAGCTGGAAG CAGAGGCTCA AGAAAACGAA GCAGATCTGA AACAATTGAA AATCAAGAAA 
CAGGAACTGC TCACGATCAA CAAGGAGATG AGCGAGCTGA TCGTCTGCCT GCAAAACGAC 
ATTTGTCTGG CGGAATCAAA GGCTCAGGGT CTCGAAGCGG AGAACAAGCT GATGAAGCAG 
GAGAAACTTG GCTACGACTG CAGATATGGG GAACTGGAGC AGCAGCTGAG CTCCGAGGCT 
GCGGAAAAGA ACGAGGAGCG GCTGCTGCTG GCCAAACATC TCTCCGAGAA GACCAAGCTA 
TACGAGTTGA CGAAGCAGAA GCTGGACAAT GTCCAGGGCG ACTTCGAGGC CACCCAGCAC 
AAGCATGCCA CCGTCGTCAA AGAGCTCCAG AGGGAGCTGC AAAAGTACAA AAAGGGCATT 
GCCGAACCCA AGGGTGGCAT TAGCTACTGC AGCAACTGCC AACAGGCCCT GAACGGGGGC 
TATCCCCAGG AGCAGCAGCA GCAACGCACC CACAGTCGCA CCAGCAGCCA TGGGAGCGTG 
CATAGCGGCA GCCGGCGGGC CAGCGAATCC TCAGAGTCCG AAACTGTGGC CAGCAGTGCG 
ACCGCTGTGC CGCCGCCTGT GATGCAACAG GTGGACCTGC AGTCGGTACC GTCCAAGAAG 
GTTCTCGTTG AGCGTATTCT CCGGCTGCAG CAGGCCACGG CTAGGCAAAC GGAACGGATC 
GAGTTCCTGG AGAACCACAC AGCCGCCCTA GTATCCGAGG TTCAGAAAAA GTCCAAAGTC 
GTCCAGCACT ACATGCTCAG GGACCAGACG GCGGGCGCCC TAACCAGCTC GAAGAGCGAC 
CAGAACAAGA GCGAGCTTAT GAAGTACGGC AACGGCATAA TGGCGGCCAT CTACGGGGGC 
TCCAAGGGCG GCGAGAGCAA GGGCATGTCC CTGGAGCTAT CGCTGGAGAT CAACAAGAAG 
CTACAGACCG TGCTAGAGGA CACGCTGCTG AAGAATATTA CGCTCAAGGA GAACCTCGAC 
ATGCTGGGCC TCGAGGTGGA CAATCTGACG CGCAAGCTGC GTGCCCTAGA GGCCTCCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001352565.2
CDS	58..2400
Translation	METAQAPPPAEERSEEKVSEMDTSQINNHLEGETTNDSGDVEQQHLECDKIEQTAERKPTKAKADDEKPVAGDELLVEHLKAAVLQQMPVPVQDEDQSGVSMRFRDLQVQDQEQNLKKLPQNEILSHVHCLAQLEEQRRSYEQQMEQLRTSNAQKDNMMTLLQRENAILDKEKQAFRKEMDMANKEKETTVIKFAMKEKLLIDAKKEKEAVDKQLADTKKEVKNVSTRFMAIHEEKSRMTYIIDEKCNEVRKYQRECEKYKTEMSHLESKLKYHMNKLSIETEAKAALERKLEEERNAPNKLEEKANEKLRMEFEANTILLKHEITSKTEALEKVTKEQQKQSEANKELQQQLQGLALEHTQLTEEFNRLREQHHGVEAAYSDELLNSAKLRGQLEELQLLRTQNTINEEKLSEQQARVQQLEAEAQENEADLKQLKIKKQELLTINKEMSELIVCLQNDICLAESKAQGLEAENKLMKQEKLGYDCRYGELEQQLSSEAAEKNEERLLLAKHLSEKTKLYELTKQKLDNVQGDFEATQHKHATVVKELQRELQKYKKGIAEPKGGISYCSNCQQALNGGYPQEQQQQRTHSRTSSHGSVHSGSRRASESSESETVASSATAVPPPVMQQVDLQSVPSKKVLVERILRLQQATARQTERIEFLENHTAALVSEVQKKSKVVQHYMLRDQTAGALTSSKSDQNKSELMKYGNGIMAAIYGGSKGGESKGMSLELSLEINKKLQTVLEDTLLKNITLKENLDMLGLEVDNLTRKLRALEASK

Target ORF information:

RefSeq Version	XM_001352529.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18529), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001352529.3

ORF Insert Sequence:

ATGGAAACAG CGCAGGCCCC CCCGCCGGCT GAGGAGAGGA GTGAAGAGAA GGTGTCTGAG 
ATGGATACTT CTCAGATAAA CAACCATCTG GAGGGAGAAA CAACAAATGA CAGTGGAGAC 
GTGGAGCAGC AGCATCTGGA ATGCGATAAA ATAGAGCAGA CTGCTGAAAG AAAGCCAACA 
AAGGCGAAAG CGGATGACGA GAAACCAGTT GCGGGAGACG AACTTCTCGT TGAGCATTTG 
AAGGCAGCGG TGCTGCAACA AATGCCTGTG CCTGTGCAGG ATGAAGACCA ATCGGGTGTC 
AGCATGCGCT TCAGAGACCT GCAAGTCCAA GACCAGGAAC AGAATCTCAA AAAGCTGCCA 
CAGAACGAGA TCCTATCCCA TGTGCACTGT CTGGCCCAGT TGGAGGAGCA GCGTCGCAGC 
TATGAGCAGC AGATGGAGCA GCTGCGAACT TCCAATGCCC AGAAGGACAA CATGATGACG 
CTCCTGCAGC GCGAGAATGC CATCTTGGAC AAGGAGAAGC AGGCCTTTCG TAAGGAAATG 
GATATGGCCA ACAAGGAGAA GGAGACCACA GTCATTAAGT TTGCCATGAA GGAGAAACTA 
CTCATCGATG CCAAAAAGGA GAAGGAGGCG GTGGATAAGC AGCTGGCGGA TACCAAGAAA 
GAGGTGAAGA ATGTTTCTAC CCGCTTCATG GCCATCCACG AGGAAAAGTC CAGAATGACG 
TACATAATAG ATGAAAAGTG CAACGAAGTT AGGAAATATC AGCGCGAATG CGAAAAGTAC 
AAAACGGAGA TGAGCCACCT AGAGTCCAAG CTAAAGTATC ACATGAACAA GCTGTCCATC 
GAAACGGAGG CCAAGGCGGC CCTGGAGCGC AAGCTGGAAG AGGAGCGAAA TGCACCGAAT 
AAGCTGGAGG AGAAGGCCAA TGAGAAGCTA AGGATGGAAT TTGAAGCAAA TACAATACTC 
TTGAAGCACG AGATCACAAG CAAGACTGAG GCCCTCGAAA AAGTCACCAA AGAGCAGCAA 
AAGCAAAGTG AAGCCAACAA AGAGCTGCAG CAGCAGCTAC AAGGGCTCGC CTTGGAGCAC 
ACTCAACTCA CGGAAGAGTT TAATCGACTA CGAGAGCAGC ACCATGGAGT CGAGGCGGCC 
TACAGCGATG AGCTGCTCAA TTCGGCCAAG CTACGGGGAC AACTCGAGGA ACTACAACTG 
CTGCGCACCC AAAACACCAT AAATGAAGAA AAGCTGTCCG AACAGCAGGC GAGAGTGCAG 
CAGCTGGAAG CAGAGGCTCA AGAAAACGAA GCAGATCTGA AACAATTGAA AATCAAGAAA 
CAGGAACTGC TCACGATCAA CAAGGAGATG AGCGAGCTGA TCGTCTGCCT GCAAAACGAC 
ATTTGTCTGG CGGAATCAAA GGCTCAGGGT CTCGAAGCGG AGAACAAGCT GATGAAGCAG 
GAGAAACTTG GCTACGACTG CAGATATGGG GAACTGGAGC AGCAGCTGAG CTCCGAGGCT 
GCGGAAAAGA ACGAGGAGCG GCTGCTGCTG GCCAAACATC TCTCCGAGAA GACCAAGCTA 
TACGAGTTGA CGAAGCAGAA GCTGGACAAT GTCCAGGGCG ACTTCGAGGC CACCCAGCAC 
AAGCATGCCA CCGTCGTCAA AGAGCTCCAG AGGGAGCTGC AAAAGTACAA AAAGGGCATT 
GCCGAACCCA AGGGTGGCAT TAGCTACTGC AGCAACTGCC AACAGGCCCT GAACGGGGGC 
TATCCCCAGG AGCAGCAGCA GCAACGCACC CACAGTCGCA CCAGCAGCCA TGGGAGCGTG 
CATAGCGGCA GCCGGCGGGC CAGCGAATCC TCAGAGTCCG AAACTGTGGC CAGCAGTGCG 
ACCGCTGTGC CGCCGCCTGT GATGCAACAG GTGGACCTGC AGTCGGTACC GTCCAAGAAG 
GTTCTCGTTG AGCGTATTCT CCGGCTGCAG CAGGCCACGG CTAGGCAAAC GGAACGGATC 
GAGTTCCTGG AGAACCACAC AGCCGCCCTA GTATCCGAGG TTCAGAAAAA GTCCAAAGTC 
GTCCAGCACT ACATGCTCAG GGACCAGACG GCGGGCGCCC TAACCAGCTC GAAGAGCGAC 
CAGAACAAGA GCGAGCTTAT GAAGTACGGC AACGGCATAA TGGCGGCCAT CTACGGGGGC 
TCCAAGGGCG GCGAGAGCAA GGGCATGTCC CTGGAGCTAT CGCTGGAGAT CAACAAGAAG 
CTACAGACCG TGCTAGAGGA CACGCTGCTG AAGAATATTA CGCTCAAGGA GAACCTCGAC 
ATGCTGGGCC TCGAGGTGGA CAATCTGACG CGCAAGCTGC GTGCCCTAGA GGCCTCCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA18529 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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