Dpse\GA17746 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA17746
Entrez Gene ID	4804040
Full Name	GA17746 gene product from transcript GA17746-RA
Synonyms	GA17746,dpse_GLEANR_14575
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001360630.3	XP_001360667.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA17746 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA17746 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe03289	XM_001360630.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA17746), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA17746 ( XM_001360630.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001360667.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA17746), mRNA.

Price & Availability↑

CloneID	ODe03289
Clone ID Related Accession (Same CDS sequence)	XM_001360630.3
Accession Version	XM_001360630.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001360630.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCTCA AAACAACGAA AAAGGAGGAA TCGCAGCTGC CCCAAGTGGG GGATTTCCTG 
GCACAGGATG GAGCTGTCCA GCAAAATACA GAAAAGAAGT ACAACTACCT GGATATGTTC 
AGATACTCCA CCAGGTTCGA GCGAGTGCTG ATCGGGTTCA GCATCGTGGT GGCTGCCGTC 
GCATCAGCAT TCATTCCCTT CTTCATGATC ATCTACGGAG AGTTCACGTC CCTGCTCGTT 
GACCGCACAG TGAGAGTGGG CACCTCCTCG CCCACATTCG CGCTGGCAAT GTTCGGAGGC 
GGAAAGCGAC TGACGAACGC CAGCAAGGAG GAGAATCAGC AGGCCATCAT CGATGACTCC 
ATCGCCTTCG GTGTAGGAAG TCTTGTGGGA TCGGTCGCCA TGTTCGTGCT CATCACCCTA 
GCCGTTGACA TTGCCAACCG GGTGGCGCTA AACCAGATCG TTCGCATCCG AAAGGTGTTC 
CTGGAGGCCA TGCTGCGGCA GGACATTACG TGGTACGACA CCACCTCCGG TACCAACTTC 
GCCAGCAAGA TGACAGAGGA TCTGGACAAG CTGAAGGAGG GAATCGGCGA GAAGGTGGTG 
ATAGTCACCT TTCTGTTCAT GACATTCGTC GTCGGCATCG TTGCTTCGTT CTTCTACGGC 
TGGGGCCTCA CACTGGTGAT TGTCGGGTGC TGTCCCTTGA TCATCATCGC CGGCACAGTG 
GTGGGCAAGA TGCAGGGCAC CCTAGCCGAA AAAGAGCTCA AGGCTTACTC GAACGCCAGC 
AATGTGGCCG AGGAGGTGTT TAGCGGAATT CGCACCGTGT TCGCCTTTAG CGGGCAAAAG 
AAGGAGAAGG ATCGTTTTGG CAAGCTACTG ATCCCCGCCG AGAACACAGG CCGCAAGAAG 
GGTCTCTACA CGGGCCTGGG CGGTGCTGCC ACCTGGCTGA TTATCTACCT TTGCATGGCC 
CTGGCCATTT GGTACGGATC GAAGCTCATT CTGGAGGATC GCAACCTCGA GGACCGCCAG 
TACACACCTG CGGTCCTTGT CATCGTCCTG TTTGCTGTCA TTATGGGAGC TCAGAATCTC 
GGTTTCGCCT CGCCGCACGT CGACTCGATG GCAGTGGCCA CTGCCGCTGG ACAAAACCTA 
TTCAGAATCA TCGATCGCCA GTCCCAGATC GATCCCATGG TCGAGATGGG GGCTAAGCCA 
GACAGCATCA CGGGACGCAT CCGCTTCGAG AACATTCACT TCCGCTATCC TGCCCGACCA 
GATGTCCAAA TCCTCAAGGG CCTGACCGTG GACGTGGAGC CGGGCCAGAC GGTGGCCTTC 
GTCGGTGCAT CGGGCTGCGG CAAGAGCACG ATGATCCAGC TGATGCAGCG GTTCTACGAC 
CCCGAGCAGG GGAGCGTCAA ACTGGATGGA CGGGATCTGC GCTCTTTGAA TGTTGGCTGG 
CTGCGCTCGC AGATCGGCAT CGTTGGCCAG GAACCGGTTC TGTTTGCCAC CACCATCGGC 
GAGAACATCC GCTATTCCCA CCCGGAAGCC ACACAGGCGG ACATAGAGCG GGCGGCACGT 
GCCGCCAACT GTCACGATTT CATCAGCAAA CTGCCCAAGG GTTACGACAC CCATGTGGGC 
GAGAAGGGCG CCCAGATTTC GGGCGGGCAG AAGCAACGCA TCGCTATTGC CCGAGCGCTG 
GTGCGGAAGC CGCAGATCCT GCTCCTGGAC GAGGCAACGT CCGCCCTGGA TCCCACATCA 
GAGAAACGGG TGCAGAGCGC CCTGGAGCTG GCCAGCCAAG GACCCACAAC TCTGGTGGTG 
GCCCATCGCC TGTCGACCAT CACGAATGCC GATAAGATCG TGTTCGTTAA GAACGGAGTG 
GTGGCCGAGC AGGGCACCCA CGAGGAGCTC ATGCAGCAAC GTGGCCTGTA CTGCGAGTTG 
GTGAACATTA CCCGGCGGAA GGAGACCACG GAAGAGGAGG AGACCGGAGA TCGAGCCCTC 
CAAAAGGCAC AGAACCTCTC CGAAGAGGAG GAGGATGACG AAACCGATGA TGATGAGCCA 
GAACTGGAAG CGGGGACCAG CAGGGAAAGT GGATTCAGCC GCGCCAGCAC CCGTCGCAAA 
CGTCGCTCGC AGCGTCGCAG CAAGAAGCAG AAGCCGGAGG CTCCCAAGTT TTCCTTCACG 
CAGCTGATGA GGCTGAATGC CCCCGAGTGG CGCTTCATTG TGGTCGGGTG CATTGCCTCG 
GTTTTGCATG GAGCAACCTT CCCGCTGTGG GGCCTTTTCT TCGGTGACTT CTTCGGGGTG 
CTCGCCAACG GAGACGACGA CGTGGTACGT GCCGAGGTCA TCAACATATC GTGCATCTTC 
ATCGGGATCG GTGTGCTGGC TGGACTGGGG ACCATGCTAC AGACCTACAT GTTTACTACG 
GCGGGCGTGA AGATGACTAC CAGGCTGCGG AACATGGCTT TCGGCACAAT TGTGTCCCAG 
GACATTGCCT ACTTCGACGA TGAGCGGAAC TCGGTGGGTG CGCTGTGCTC CCGCCTGGCC 
AGTGACTGCT CCAACGTACA GGGGGCTACC GGTGCTCGCG TTGGAGTCAT GCTGCAAGCC 
GTCTCCACTT TGGGCATCGG CATGGTCGTA GGTTTTGTCT TCTCTTGGCA ACAGACGCTT 
CTCACGCTGG TCACCCTGCC GCTTGTCTGC CTGTCGGTGT ACCTGGAAGG TCGCTTCATC 
ATGAAGAGCG CCCAGTCAGC CAAGGCGGCC GTCGAGGAGG CTTCGCAGGT GGCTGTGGAG 
GCAATAACCA ACATTCGGAC TGTGAACGGA CTGAACCTGG AGCGTCGCAT CCTGGAAACC 
TACGTACAAC AGATCGACAA CGTGGATGTT GCCTGTCGTC GCAAGGTTCG TTTCCGTGGC 
GTGGTCTTTG CGCTGGGACA GGCGGCTCCC TTCCTGGCCT ATGGCATATC CATGTACTAC 
GGTGGACTGC TCGTGGCCGA TGAGGCTATC AATTATGAGG ACATTATCAA GGTGGCGGAA 
GCCCTGATCT TCGGTTCTTG GATGCTGGGC CAGGCGTTGG CCTATGCCCC GAACGTGAAC 
GATGCCATCC TTTCCGCTGG CCGCCTCATG GAGCTGTTTA AGAGCAACTC CACTCAACCC 
AATCCGCCAG AGAATCCCTA CAACACCGTG GAGAAATCTG AGGGCGACAT TGTGTACGAG 
AACGTGGGCT TTGAGTATCC GACCCGGAAG GGGACTCCCA TACTGCAGAA CCTGAATCTC 
TCCATCAAGA AGTCGACGAC TGTGGCTCTC GTAGGTCCGT CAGGTTCTGG CAAATCGACC 
TGCGTGCAGC TGCTGCTCCG CTATTATGAC CCCGTTTCCG GATCGGTGAA TCTCAGTGGA 
GTGCCGAGCA CAGACTTCCC TCTGGACACG CTGCGCTCCA AGCTGGGGCT GGTCTCGCAG 
GAACCCGTGC TCTTCGACCG CACTATTGCC GAGAACATTG CGTACGGGAA CAACTTCCGG 
GACGAGGTCT CCATGCAGGA GATCATCGAA GCGGCCAAAA AGTCCAACAT CCACAACTTT 
GTCAGCTCTT TGCCGCAGGG CTACGAAACG CGGCTTGGCA AGTCGTCGCA GCTCTCTGGA 
GGCCAGAAGC AGCGCATTGC CATTGCCCGG GCTCTGGTGA GAAATCCCAA GATTCTGATA 
CTTGACGAGG CCACATCTGC TCTGGATCTG GAGAGCGAGA AGGTTGTTCA GCAGGCCCTG 
GACGAGGCTC GTTCTGGACG TACCTGTCTC ACGATTGCCC ACCGCCTAAC CACAGTACGG 
AACGCGGACC TGATCTGCGT CCTCAAGAGA GGTGTGGTCG TTGAGCATGG TACGCATGAA 
GAGCTGATGG CTCTGAATCG CATATACGCT AATCTCTACC TGATGCAGCA GGTGGCGGGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001360667.2
CDS	222..4124
Translation	MDLKTTKKEESQLPQVGDFLAQDGAVQQNTEKKYNYLDMFRYSTRFERVLIGFSIVVAAVASAFIPFFMIIYGEFTSLLVDRTVRVGTSSPTFALAMFGGGKRLTNASKEENQQAIIDDSIAFGVGSLVGSVAMFVLITLAVDIANRVALNQIVRIRKVFLEAMLRQDITWYDTTSGTNFASKMTEDLDKLKEGIGEKVVIVTFLFMTFVVGIVASFFYGWGLTLVIVGCCPLIIIAGTVVGKMQGTLAEKELKAYSNASNVAEEVFSGIRTVFAFSGQKKEKDRFGKLLIPAENTGRKKGLYTGLGGAATWLIIYLCMALAIWYGSKLILEDRNLEDRQYTPAVLVIVLFAVIMGAQNLGFASPHVDSMAVATAAGQNLFRIIDRQSQIDPMVEMGAKPDSITGRIRFENIHFRYPARPDVQILKGLTVDVEPGQTVAFVGASGCGKSTMIQLMQRFYDPEQGSVKLDGRDLRSLNVGWLRSQIGIVGQEPVLFATTIGENIRYSHPEATQADIERAARAANCHDFISKLPKGYDTHVGEKGAQISGGQKQRIAIARALVRKPQILLLDEATSALDPTSEKRVQSALELASQGPTTLVVAHRLSTITNADKIVFVKNGVVAEQGTHEELMQQRGLYCELVNITRRKETTEEEETGDRALQKAQNLSEEEEDDETDDDEPELEAGTSRESGFSRASTRRKRRSQRRSKKQKPEAPKFSFTQLMRLNAPEWRFIVVGCIASVLHGATFPLWGLFFGDFFGVLANGDDDVVRAEVINISCIFIGIGVLAGLGTMLQTYMFTTAGVKMTTRLRNMAFGTIVSQDIAYFDDERNSVGALCSRLASDCSNVQGATGARVGVMLQAVSTLGIGMVVGFVFSWQQTLLTLVTLPLVCLSVYLEGRFIMKSAQSAKAAVEEASQVAVEAITNIRTVNGLNLERRILETYVQQIDNVDVACRRKVRFRGVVFALGQAAPFLAYGISMYYGGLLVADEAINYEDIIKVAEALIFGSWMLGQALAYAPNVNDAILSAGRLMELFKSNSTQPNPPENPYNTVEKSEGDIVYENVGFEYPTRKGTPILQNLNLSIKKSTTVALVGPSGSGKSTCVQLLLRYYDPVSGSVNLSGVPSTDFPLDTLRSKLGLVSQEPVLFDRTIAENIAYGNNFRDEVSMQEIIEAAKKSNIHNFVSSLPQGYETRLGKSSQLSGGQKQRIAIARALVRNPKILILDEATSALDLESEKVVQQALDEARSGRTCLTIAHRLTTVRNADLICVLKRGVVVEHGTHEELMALNRIYANLYLMQQVAG

Target ORF information:

RefSeq Version	XM_001360630.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA17746), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001360630.3

ORF Insert Sequence:

ATGGATCTCA AAACAACGAA AAAGGAGGAA TCGCAGCTGC CCCAAGTGGG GGATTTCCTG 
GCACAGGATG GAGCTGTCCA GCAAAATACA GAAAAGAAGT ACAACTACCT GGATATGTTC 
AGATACTCCA CCAGGTTCGA GCGAGTGCTG ATCGGGTTCA GCATCGTGGT GGCTGCCGTC 
GCATCAGCAT TCATTCCCTT CTTCATGATC ATCTACGGAG AGTTCACGTC CCTGCTCGTT 
GACCGCACAG TGAGAGTGGG CACCTCCTCG CCCACATTCG CGCTGGCAAT GTTCGGAGGC 
GGAAAGCGAC TGACGAACGC CAGCAAGGAG GAGAATCAGC AGGCCATCAT CGATGACTCC 
ATCGCCTTCG GTGTAGGAAG TCTTGTGGGA TCGGTCGCCA TGTTCGTGCT CATCACCCTA 
GCCGTTGACA TTGCCAACCG GGTGGCGCTA AACCAGATCG TTCGCATCCG AAAGGTGTTC 
CTGGAGGCCA TGCTGCGGCA GGACATTACG TGGTACGACA CCACCTCCGG TACCAACTTC 
GCCAGCAAGA TGACAGAGGA TCTGGACAAG CTGAAGGAGG GAATCGGCGA GAAGGTGGTG 
ATAGTCACCT TTCTGTTCAT GACATTCGTC GTCGGCATCG TTGCTTCGTT CTTCTACGGC 
TGGGGCCTCA CACTGGTGAT TGTCGGGTGC TGTCCCTTGA TCATCATCGC CGGCACAGTG 
GTGGGCAAGA TGCAGGGCAC CCTAGCCGAA AAAGAGCTCA AGGCTTACTC GAACGCCAGC 
AATGTGGCCG AGGAGGTGTT TAGCGGAATT CGCACCGTGT TCGCCTTTAG CGGGCAAAAG 
AAGGAGAAGG ATCGTTTTGG CAAGCTACTG ATCCCCGCCG AGAACACAGG CCGCAAGAAG 
GGTCTCTACA CGGGCCTGGG CGGTGCTGCC ACCTGGCTGA TTATCTACCT TTGCATGGCC 
CTGGCCATTT GGTACGGATC GAAGCTCATT CTGGAGGATC GCAACCTCGA GGACCGCCAG 
TACACACCTG CGGTCCTTGT CATCGTCCTG TTTGCTGTCA TTATGGGAGC TCAGAATCTC 
GGTTTCGCCT CGCCGCACGT CGACTCGATG GCAGTGGCCA CTGCCGCTGG ACAAAACCTA 
TTCAGAATCA TCGATCGCCA GTCCCAGATC GATCCCATGG TCGAGATGGG GGCTAAGCCA 
GACAGCATCA CGGGACGCAT CCGCTTCGAG AACATTCACT TCCGCTATCC TGCCCGACCA 
GATGTCCAAA TCCTCAAGGG CCTGACCGTG GACGTGGAGC CGGGCCAGAC GGTGGCCTTC 
GTCGGTGCAT CGGGCTGCGG CAAGAGCACG ATGATCCAGC TGATGCAGCG GTTCTACGAC 
CCCGAGCAGG GGAGCGTCAA ACTGGATGGA CGGGATCTGC GCTCTTTGAA TGTTGGCTGG 
CTGCGCTCGC AGATCGGCAT CGTTGGCCAG GAACCGGTTC TGTTTGCCAC CACCATCGGC 
GAGAACATCC GCTATTCCCA CCCGGAAGCC ACACAGGCGG ACATAGAGCG GGCGGCACGT 
GCCGCCAACT GTCACGATTT CATCAGCAAA CTGCCCAAGG GTTACGACAC CCATGTGGGC 
GAGAAGGGCG CCCAGATTTC GGGCGGGCAG AAGCAACGCA TCGCTATTGC CCGAGCGCTG 
GTGCGGAAGC CGCAGATCCT GCTCCTGGAC GAGGCAACGT CCGCCCTGGA TCCCACATCA 
GAGAAACGGG TGCAGAGCGC CCTGGAGCTG GCCAGCCAAG GACCCACAAC TCTGGTGGTG 
GCCCATCGCC TGTCGACCAT CACGAATGCC GATAAGATCG TGTTCGTTAA GAACGGAGTG 
GTGGCCGAGC AGGGCACCCA CGAGGAGCTC ATGCAGCAAC GTGGCCTGTA CTGCGAGTTG 
GTGAACATTA CCCGGCGGAA GGAGACCACG GAAGAGGAGG AGACCGGAGA TCGAGCCCTC 
CAAAAGGCAC AGAACCTCTC CGAAGAGGAG GAGGATGACG AAACCGATGA TGATGAGCCA 
GAACTGGAAG CGGGGACCAG CAGGGAAAGT GGATTCAGCC GCGCCAGCAC CCGTCGCAAA 
CGTCGCTCGC AGCGTCGCAG CAAGAAGCAG AAGCCGGAGG CTCCCAAGTT TTCCTTCACG 
CAGCTGATGA GGCTGAATGC CCCCGAGTGG CGCTTCATTG TGGTCGGGTG CATTGCCTCG 
GTTTTGCATG GAGCAACCTT CCCGCTGTGG GGCCTTTTCT TCGGTGACTT CTTCGGGGTG 
CTCGCCAACG GAGACGACGA CGTGGTACGT GCCGAGGTCA TCAACATATC GTGCATCTTC 
ATCGGGATCG GTGTGCTGGC TGGACTGGGG ACCATGCTAC AGACCTACAT GTTTACTACG 
GCGGGCGTGA AGATGACTAC CAGGCTGCGG AACATGGCTT TCGGCACAAT TGTGTCCCAG 
GACATTGCCT ACTTCGACGA TGAGCGGAAC TCGGTGGGTG CGCTGTGCTC CCGCCTGGCC 
AGTGACTGCT CCAACGTACA GGGGGCTACC GGTGCTCGCG TTGGAGTCAT GCTGCAAGCC 
GTCTCCACTT TGGGCATCGG CATGGTCGTA GGTTTTGTCT TCTCTTGGCA ACAGACGCTT 
CTCACGCTGG TCACCCTGCC GCTTGTCTGC CTGTCGGTGT ACCTGGAAGG TCGCTTCATC 
ATGAAGAGCG CCCAGTCAGC CAAGGCGGCC GTCGAGGAGG CTTCGCAGGT GGCTGTGGAG 
GCAATAACCA ACATTCGGAC TGTGAACGGA CTGAACCTGG AGCGTCGCAT CCTGGAAACC 
TACGTACAAC AGATCGACAA CGTGGATGTT GCCTGTCGTC GCAAGGTTCG TTTCCGTGGC 
GTGGTCTTTG CGCTGGGACA GGCGGCTCCC TTCCTGGCCT ATGGCATATC CATGTACTAC 
GGTGGACTGC TCGTGGCCGA TGAGGCTATC AATTATGAGG ACATTATCAA GGTGGCGGAA 
GCCCTGATCT TCGGTTCTTG GATGCTGGGC CAGGCGTTGG CCTATGCCCC GAACGTGAAC 
GATGCCATCC TTTCCGCTGG CCGCCTCATG GAGCTGTTTA AGAGCAACTC CACTCAACCC 
AATCCGCCAG AGAATCCCTA CAACACCGTG GAGAAATCTG AGGGCGACAT TGTGTACGAG 
AACGTGGGCT TTGAGTATCC GACCCGGAAG GGGACTCCCA TACTGCAGAA CCTGAATCTC 
TCCATCAAGA AGTCGACGAC TGTGGCTCTC GTAGGTCCGT CAGGTTCTGG CAAATCGACC 
TGCGTGCAGC TGCTGCTCCG CTATTATGAC CCCGTTTCCG GATCGGTGAA TCTCAGTGGA 
GTGCCGAGCA CAGACTTCCC TCTGGACACG CTGCGCTCCA AGCTGGGGCT GGTCTCGCAG 
GAACCCGTGC TCTTCGACCG CACTATTGCC GAGAACATTG CGTACGGGAA CAACTTCCGG 
GACGAGGTCT CCATGCAGGA GATCATCGAA GCGGCCAAAA AGTCCAACAT CCACAACTTT 
GTCAGCTCTT TGCCGCAGGG CTACGAAACG CGGCTTGGCA AGTCGTCGCA GCTCTCTGGA 
GGCCAGAAGC AGCGCATTGC CATTGCCCGG GCTCTGGTGA GAAATCCCAA GATTCTGATA 
CTTGACGAGG CCACATCTGC TCTGGATCTG GAGAGCGAGA AGGTTGTTCA GCAGGCCCTG 
GACGAGGCTC GTTCTGGACG TACCTGTCTC ACGATTGCCC ACCGCCTAAC CACAGTACGG 
AACGCGGACC TGATCTGCGT CCTCAAGAGA GGTGTGGTCG TTGAGCATGG TACGCATGAA 
GAGCTGATGG CTCTGAATCG CATATACGCT AATCTCTACC TGATGCAGCA GGTGGCGGGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA17746 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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