Dpse\GA18275 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA18275
Entrez Gene ID	4803287
Full Name	GA18275 gene product from transcript GA18275-RA
Synonyms	GA18275,dpse_GLEANR_2889
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001360015.3	XP_001360052.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA18275 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA18275 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe20006	XM_001360015.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18275), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA18275 ( XM_001360015.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001360052.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18275), mRNA.

Price & Availability↑

CloneID	ODe20006
Clone ID Related Accession (Same CDS sequence)	XM_001360015.3
Accession Version	XM_001360015.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001360015.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGT GCAAAATGGG ATTCTACGTT TTAGTTTGTA GCGTGTTGTT TTTACTTGCC 
AGCGGCTCGA TTGCATCAGC TAAGGCCGCC GACTCGGCAG AAGTTGTGAG TGGCAGCAGC 
AGCAGCAGCG TTGAAGAAAA GACGGACTGC ACGGACCTGG CCCGCGATGA GGAGGCCCTG 
ATGGTGTTCT CCACTTTGGG TGGCGGTCTC ACGGCCATCG ATCCGATTAC AAGTGAAATA 
CGCTGGACAA TAGCAGATGA TCCCCCCATT GTAGCCGAGC CGCAGGAGAA CCTCCAAGTG 
CCACACTTTC TGCCAGATCC ACGAGATGGC AGCATCTATC AGCTGGGGCA GATGGGCAGC 
CTCAAGAAGC TGCCTTACAC CATACCCCAA CTGGTGGCCA ATGCACCGTG TCGCTCCTCG 
GACGGGATAC TGTACTCCGG GAAGAAGAGC GACACCTGGT ACATGGTGGA CCCGAAGACA 
GGCAGACGCG AGAAGGTCAT GGGCTTTGGG GATGCCACAA TGGATGGCAA GGAGGGCGAA 
CAGATTGGCT GGGCCACCTC GCGGGCCATT TATTTGGGCA GAACACAGTA CACAGTGATG 
ATGTTCGATA GCTTGGCCAA GGACAAGAAT GCCAAGCCAT GGAATATAAC ATTTTATGAC 
TACAATGCGC TGAGTGCGCC ACCAGAATTG GTCAAGGAAT ATGAATACAT CCACTTGACG 
ACCACCACCA ATGGACAGAT CGTGACCCTA GATCGCAAAC ATGGGAAATT CTTGTGGCAG 
CGAGATCTGA GCAGTCCCGT AGTGGCTGCC TTCCTGCTGG GGCCCGATGG GCTGCTCAGT 
GTGCCTTTTA CCACGCTCTC GGATGAGGCC TATCAGGCGA TACTCGAGGA ATCCAAAACA 
GGGAATGTAA ACACCGTGAA ACTATTTCAA TCGTTGTATG TGGGCGAGCA CCAGAGCGGT 
TTGTATGCCA TTCCCTCGCT GGTGGACAAG AATACGGCCA GAATATCGAC CACACCACCC 
GTAAAACTCC TCGATGGACC CGTTGGGGAG CAAAATACCA ACAAGGAAAC GGATCCCAAG 
ACCGTTTACA TCAATGATGT GCTGCGCGAG CACTCGGGCA TCATGTTGGG CCACTATAAT 
ATGCCCAATG AAGCGAATGC CAATCTGCAG CTATCCCCCA CGCCAGCGGG TGGCAAAGAT 
GTCCAGAGTC TGGCGACTAT CGACAACTAT AATGATGGCT ATGGGCTGTT GGCCAACAAT 
GAGAAGAATG CCGCTGATAT TGGCGTGCAA ACGGATCCGG AACTGGTGGA GATTGGCATT 
AATCAGCGGA CAAACAGCAA TACCATCAAT CGGACAAAGA CCATCATCCT GCAGAACAGC 
AACAAGGTTC AGGCCTTCAT CAATGAGTGG TTCATGGAAC ATCCAAGCGG AAAGGTCCAT 
CAGATACTGA TTGTGATCGT TCTGGGTATG ATTGCCTTGT TCTGGTACAC CTGCAGCACT 
ATGCGGGAGC TGCAGAAGCA GAGCGAAAAT GGATCCAAAA CGCTGGCCAT GACTGCGGAT 
GGCATAAACG GAAGCGGCAG CAAGGGGAGC AATGGCAGCA ACATCAGTGC CGAGGATCTG 
GTTGATCTGG GCAATGGCCA GGTGCGTGTC GGAAAGATTA GCTTCAATTC CAACGAAGTA 
CTGGGCAAGG GCTGCGAGGG CACTTTCGTC TTCAAGGGAT CTTTCGAGGA GAGGTCTGTG 
GCCGTCAAGC GACTCCTTCC CGAATGCTTC ACCTTTGCGG ATCGCGAAGT GGCGCTGCTA 
CGCGAATCGG ATGCCCATGA GAATGTTGTA CGCTATTTCT GCACCGAGCA GGATCGTCAG 
TTCCGCTACA TAGCCGTGGA GCTGTGTGCC GCCACGCTGC AGGACTACAC CGAAGGCGAT 
CGTTCGGTGG AGCTGCAGAA TCACATTGAT GTGTGGCAGG TTCTGGGACA AGCAGCCTCC 
GGATTGAGTC ATCTGCATTC CCTGGACATT GTCCATCGTG ATATCAAGCC ACAGAATGTT 
CTGATCTCCC TGCCCGATGC CAGGGGCAAG GTACGAGTGA TGATCTCTGA TTTTGGTTTG 
TGCAAAAAGT TGAATTTTGG AAAGACAAGC TTCTCCCGTC GGTCGGGCGT TACGGGAACA 
GATGGTTGGA TAGCTCCCGA AATGATGAGG GCACAACGAA CGACCACTGC TGTGGATATC 
TTCTCGCTGG GCTGCGTCTA CTATTATGTC CTGAGTGGGG GACATCATGC CTTTGGAGAC 
AATCTAAAGC GCCAGGCAAA CATACTCTCG CATGAGTACA ATCTGTCGAA GCTGCGTTCC 
GATGATGACA GCGACAATAG CAAGACTGTT CTGGCTGAAC AACTGATTTC CGACATGATA 
CACAAGGATG CCCAGTCGCG TCCTCCCGCA CGCTGTATAG GCAATCATCC TTTGTTTTGG 
GATGAGCCCA AAATGCTCTC CTTCCTGCAG GACGTGAGCG ATCGTGTGGA GAAACTGCAG 
TTCCATACCG AGCCCCTCAA ATCGCTGGAG AAGAATGGCC GGATTGTGGT CTTGGACGAT 
TGGAATGAGC ATTTGGATCC CATGATCACC GATGACCTGC GAAAGTATCG CGGGTATATG 
GGCGCCAGTG TGAGAGATCT GTTGAGGGCG CTGCGCAACA AGAAGCATCA CTATCATGAG 
CTGACGCCAG CGGCCCAGGA GATGCTCGGC TGCATACCGC ACGAGTTCAC CAACTACTGG 
GTGGAACGCT TCCCGCAGCT CATCTCGCAC GCGTATCACG CCTTCAGTAT TTGCTCGAAC 
GAGCCCATCT TCAAGCCTTA CTACAGTGCT GGCTATCATT TTTCGCGTCC CTGGTACTTT 
GAGGCCGACG ATGCGCTCTT TCCCCTGCTG ATGCGGGATC CCAAGCCACT GCCGAAGATT 
GGCAGCCCCA AGAAGACGCC TCCATCCACG GCCAGTCAGC AGCTGCAGCA GCAGTTGAAG 
CTGCGCAAGG GTTCGTATAA TTTCCGCAAA GCCAGCGATG AGCTGCCCAT CGCGGGAGTC 
GGTCTGCAGC GGAACCTAGA GCTGGACGCC AGCCACCAGC CCCTGGCGCT GGAGGGTGAT 
CCCAAGCGGG ATGTGTTTGC CAATTTCAAG TTCCGTCGCT CGAAGGCGTC CGCTAATCGC 
AGCACCTACA AGGACACGCA GGAGAAGGAG AAGTACGTGA GCTGGACTTT GCCGCCCACA 
ACGCAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001360052.3
CDS	250..3501
Translation	MMLCKMGFYVLVCSVLFLLASGSIASAKAADSAEVVSGSSSSSVEEKTDCTDLARDEEALMVFSTLGGGLTAIDPITSEIRWTIADDPPIVAEPQENLQVPHFLPDPRDGSIYQLGQMGSLKKLPYTIPQLVANAPCRSSDGILYSGKKSDTWYMVDPKTGRREKVMGFGDATMDGKEGEQIGWATSRAIYLGRTQYTVMMFDSLAKDKNAKPWNITFYDYNALSAPPELVKEYEYIHLTTTTNGQIVTLDRKHGKFLWQRDLSSPVVAAFLLGPDGLLSVPFTTLSDEAYQAILEESKTGNVNTVKLFQSLYVGEHQSGLYAIPSLVDKNTARISTTPPVKLLDGPVGEQNTNKETDPKTVYINDVLREHSGIMLGHYNMPNEANANLQLSPTPAGGKDVQSLATIDNYNDGYGLLANNEKNAADIGVQTDPELVEIGINQRTNSNTINRTKTIILQNSNKVQAFINEWFMEHPSGKVHQILIVIVLGMIALFWYTCSTMRELQKQSENGSKTLAMTADGINGSGSKGSNGSNISAEDLVDLGNGQVRVGKISFNSNEVLGKGCEGTFVFKGSFEERSVAVKRLLPECFTFADREVALLRESDAHENVVRYFCTEQDRQFRYIAVELCAATLQDYTEGDRSVELQNHIDVWQVLGQAASGLSHLHSLDIVHRDIKPQNVLISLPDARGKVRVMISDFGLCKKLNFGKTSFSRRSGVTGTDGWIAPEMMRAQRTTTAVDIFSLGCVYYYVLSGGHHAFGDNLKRQANILSHEYNLSKLRSDDDSDNSKTVLAEQLISDMIHKDAQSRPPARCIGNHPLFWDEPKMLSFLQDVSDRVEKLQFHTEPLKSLEKNGRIVVLDDWNEHLDPMITDDLRKYRGYMGASVRDLLRALRNKKHHYHELTPAAQEMLGCIPHEFTNYWVERFPQLISHAYHAFSICSNEPIFKPYYSAGYHFSRPWYFEADDALFPLLMRDPKPLPKIGSPKKTPPSTASQQLQQQLKLRKGSYNFRKASDELPIAGVGLQRNLELDASHQPLALEGDPKRDVFANFKFRRSKASANRSTYKDTQEKEKYVSWTLPPTTQD

Target ORF information:

RefSeq Version	XM_001360015.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18275), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001360015.3

ORF Insert Sequence:

ATGATGTTGT GCAAAATGGG ATTCTACGTT TTAGTTTGTA GCGTGTTGTT TTTACTTGCC 
AGCGGCTCGA TTGCATCAGC TAAGGCCGCC GACTCGGCAG AAGTTGTGAG TGGCAGCAGC 
AGCAGCAGCG TTGAAGAAAA GACGGACTGC ACGGACCTGG CCCGCGATGA GGAGGCCCTG 
ATGGTGTTCT CCACTTTGGG TGGCGGTCTC ACGGCCATCG ATCCGATTAC AAGTGAAATA 
CGCTGGACAA TAGCAGATGA TCCCCCCATT GTAGCCGAGC CGCAGGAGAA CCTCCAAGTG 
CCACACTTTC TGCCAGATCC ACGAGATGGC AGCATCTATC AGCTGGGGCA GATGGGCAGC 
CTCAAGAAGC TGCCTTACAC CATACCCCAA CTGGTGGCCA ATGCACCGTG TCGCTCCTCG 
GACGGGATAC TGTACTCCGG GAAGAAGAGC GACACCTGGT ACATGGTGGA CCCGAAGACA 
GGCAGACGCG AGAAGGTCAT GGGCTTTGGG GATGCCACAA TGGATGGCAA GGAGGGCGAA 
CAGATTGGCT GGGCCACCTC GCGGGCCATT TATTTGGGCA GAACACAGTA CACAGTGATG 
ATGTTCGATA GCTTGGCCAA GGACAAGAAT GCCAAGCCAT GGAATATAAC ATTTTATGAC 
TACAATGCGC TGAGTGCGCC ACCAGAATTG GTCAAGGAAT ATGAATACAT CCACTTGACG 
ACCACCACCA ATGGACAGAT CGTGACCCTA GATCGCAAAC ATGGGAAATT CTTGTGGCAG 
CGAGATCTGA GCAGTCCCGT AGTGGCTGCC TTCCTGCTGG GGCCCGATGG GCTGCTCAGT 
GTGCCTTTTA CCACGCTCTC GGATGAGGCC TATCAGGCGA TACTCGAGGA ATCCAAAACA 
GGGAATGTAA ACACCGTGAA ACTATTTCAA TCGTTGTATG TGGGCGAGCA CCAGAGCGGT 
TTGTATGCCA TTCCCTCGCT GGTGGACAAG AATACGGCCA GAATATCGAC CACACCACCC 
GTAAAACTCC TCGATGGACC CGTTGGGGAG CAAAATACCA ACAAGGAAAC GGATCCCAAG 
ACCGTTTACA TCAATGATGT GCTGCGCGAG CACTCGGGCA TCATGTTGGG CCACTATAAT 
ATGCCCAATG AAGCGAATGC CAATCTGCAG CTATCCCCCA CGCCAGCGGG TGGCAAAGAT 
GTCCAGAGTC TGGCGACTAT CGACAACTAT AATGATGGCT ATGGGCTGTT GGCCAACAAT 
GAGAAGAATG CCGCTGATAT TGGCGTGCAA ACGGATCCGG AACTGGTGGA GATTGGCATT 
AATCAGCGGA CAAACAGCAA TACCATCAAT CGGACAAAGA CCATCATCCT GCAGAACAGC 
AACAAGGTTC AGGCCTTCAT CAATGAGTGG TTCATGGAAC ATCCAAGCGG AAAGGTCCAT 
CAGATACTGA TTGTGATCGT TCTGGGTATG ATTGCCTTGT TCTGGTACAC CTGCAGCACT 
ATGCGGGAGC TGCAGAAGCA GAGCGAAAAT GGATCCAAAA CGCTGGCCAT GACTGCGGAT 
GGCATAAACG GAAGCGGCAG CAAGGGGAGC AATGGCAGCA ACATCAGTGC CGAGGATCTG 
GTTGATCTGG GCAATGGCCA GGTGCGTGTC GGAAAGATTA GCTTCAATTC CAACGAAGTA 
CTGGGCAAGG GCTGCGAGGG CACTTTCGTC TTCAAGGGAT CTTTCGAGGA GAGGTCTGTG 
GCCGTCAAGC GACTCCTTCC CGAATGCTTC ACCTTTGCGG ATCGCGAAGT GGCGCTGCTA 
CGCGAATCGG ATGCCCATGA GAATGTTGTA CGCTATTTCT GCACCGAGCA GGATCGTCAG 
TTCCGCTACA TAGCCGTGGA GCTGTGTGCC GCCACGCTGC AGGACTACAC CGAAGGCGAT 
CGTTCGGTGG AGCTGCAGAA TCACATTGAT GTGTGGCAGG TTCTGGGACA AGCAGCCTCC 
GGATTGAGTC ATCTGCATTC CCTGGACATT GTCCATCGTG ATATCAAGCC ACAGAATGTT 
CTGATCTCCC TGCCCGATGC CAGGGGCAAG GTACGAGTGA TGATCTCTGA TTTTGGTTTG 
TGCAAAAAGT TGAATTTTGG AAAGACAAGC TTCTCCCGTC GGTCGGGCGT TACGGGAACA 
GATGGTTGGA TAGCTCCCGA AATGATGAGG GCACAACGAA CGACCACTGC TGTGGATATC 
TTCTCGCTGG GCTGCGTCTA CTATTATGTC CTGAGTGGGG GACATCATGC CTTTGGAGAC 
AATCTAAAGC GCCAGGCAAA CATACTCTCG CATGAGTACA ATCTGTCGAA GCTGCGTTCC 
GATGATGACA GCGACAATAG CAAGACTGTT CTGGCTGAAC AACTGATTTC CGACATGATA 
CACAAGGATG CCCAGTCGCG TCCTCCCGCA CGCTGTATAG GCAATCATCC TTTGTTTTGG 
GATGAGCCCA AAATGCTCTC CTTCCTGCAG GACGTGAGCG ATCGTGTGGA GAAACTGCAG 
TTCCATACCG AGCCCCTCAA ATCGCTGGAG AAGAATGGCC GGATTGTGGT CTTGGACGAT 
TGGAATGAGC ATTTGGATCC CATGATCACC GATGACCTGC GAAAGTATCG CGGGTATATG 
GGCGCCAGTG TGAGAGATCT GTTGAGGGCG CTGCGCAACA AGAAGCATCA CTATCATGAG 
CTGACGCCAG CGGCCCAGGA GATGCTCGGC TGCATACCGC ACGAGTTCAC CAACTACTGG 
GTGGAACGCT TCCCGCAGCT CATCTCGCAC GCGTATCACG CCTTCAGTAT TTGCTCGAAC 
GAGCCCATCT TCAAGCCTTA CTACAGTGCT GGCTATCATT TTTCGCGTCC CTGGTACTTT 
GAGGCCGACG ATGCGCTCTT TCCCCTGCTG ATGCGGGATC CCAAGCCACT GCCGAAGATT 
GGCAGCCCCA AGAAGACGCC TCCATCCACG GCCAGTCAGC AGCTGCAGCA GCAGTTGAAG 
CTGCGCAAGG GTTCGTATAA TTTCCGCAAA GCCAGCGATG AGCTGCCCAT CGCGGGAGTC 
GGTCTGCAGC GGAACCTAGA GCTGGACGCC AGCCACCAGC CCCTGGCGCT GGAGGGTGAT 
CCCAAGCGGG ATGTGTTTGC CAATTTCAAG TTCCGTCGCT CGAAGGCGTC CGCTAATCGC 
AGCACCTACA AGGACACGCA GGAGAAGGAG AAGTACGTGA GCTGGACTTT GCCGCCCACA 
ACGCAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA18275 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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