Dpse\GA18534 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA18534
Entrez Gene ID	4801524
Full Name	GA18534 gene product from transcript GA18534-RA
Synonyms	GA18534,dpse_GLEANR_5489
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001358563.3	XP_001358600.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA18534 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA18534 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe01125	XM_001358563.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18534), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA18534 ( XM_001358563.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001358600.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18534), mRNA.

Price & Availability↑

CloneID	ODe01125
Clone ID Related Accession (Same CDS sequence)	XM_001358563.3
Accession Version	XM_001358563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001358563.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGA AGATTACGCT TGCCGATGCA CTGTCCAATG TGGAGGTGCT GGACGAGCTG 
TCGCTGCCCG ATGAGCAGCC ATGTATAGAG GCGCAGCCCT GCTCCATTAT CTACAAGGCA 
AACTTCGATA CAAACTTTGA AGATCGCAAT GGATTTGTCA CTGGAATTGC CAAGTACATA 
GAGGAAGCGA CAACGCACGC GAATTTGAAT GTTTTGCTCG ATGAGGGGCA GAAACATGCA 
GTCATGCTTT ACACCTGGCG ATGCTGCTCT CGGGCCATAC CACAGCCCAA GTCGAACGAG 
CAGCCCAATC GTGTGGAGAT CTATGAGAAG ACGGTCGAAG TGCTTGCCCC AGAAGTGAAC 
AAGCTGCTGA ACTTTATGTA TTTCCAACGC AAAGCCATCG AGGCCTTTTC GGGGGAGGTG 
AAGCGGCTGT GCCACGCAGA GAAGCGCAAG GATTTTGTGT CGGAAGCATA CTTGTTGACG 
CTGGGAAAAT TCATCAACAT GTTTGCTGTT CTTGACGAGC TGAAGAACAT GAAGTCGAGT 
GTAAAAAACG ATTATTCCAC CTATAGGCGG GCAGCTCAAT TCCTGAAGGT GATGTCCGAC 
TCGCACACAC TGCAGGAGTC GCAGAATCTC TCCATGTTCC TGGCCACGCA GAACAAAATA 
CGCGACACTG TCAAGGACAC GCTGGAAAAG ATCGTCGGCT ACGAGGATCT GCTCTCGGAT 
GTCGTTAATA TCTGTGTGCA CATGTTCGAG ACGAAAATGT ACTTGACGCC CGAGGAGAAG 
CATATGCTGG TGAAGGTAAT GGGCTTTGGC CTCTTCCTCA TGGACAGTGA TGCCTGCAAC 
ATCAACAAGT TGGACCAAAA GAAGAAAATC CGCCTGGACC GCATCGATCG CATCTTTAAG 
AACCTGGAGG TGGTGCCCCT CTTCGGTGAT ATGCAGATAG CACCCTTCAA CTATATAAAG 
CGGAGCAAGC ACTTCGACTC CAGCAAGTGG CCGCTCTCCA GCTCGAATGC CATCAGCCCT 
CAGGCTGATT TGATGGTGCA CTTGCCGCAG ATACGGGAGG ATCATGTCAA GTACATATCA 
GAGCTGGCGC GATACACCAA CGAGGTCACC ACCACTGTCA AGGAGAATCC ATCGGATGCT 
GAGAATCGGA TTACGGCCGA CTTGGCCCTG CGCGGTCTGC AGCTTCTGTC GGAGTGGACA 
AGTGTGGTCA CTGAGCTGTA CTCCTGGAAG CTGCTCCATC CCACAGACCA CCACCAGAAC 
AAGGAGTGTC CCGTGGAGGC CGAGGAGTAC GAAAGGGCAA CCCGATACAA TTACACTTCC 
GAGGAGAAGT TTGCCCTGAT TGAAGTGATC GCCATGATCA AAGGCCTGCA GGTGCTGATG 
GCACGCATCG AGACGGTGCT GTGTGAGGCT ATTCGACGCA ATATTTATTC CGAGCTGCAG 
GACTTTGTTC AGCTATCGCT GCGCGAACCC CTTCGGAAGG CGGTGAAGAA CAAAAAGGAT 
CTAATCCGGA GCATCATTAT GTCGGTGCGG GAGACATCTG CGGATTGGCA AAAGGGCTAC 
GAGCCCACCG ACGATCCCGT GTCCAAAGGC AAAAAAGATC CCGATGGCGG CTTTCGTATC 
CATGTGCCAC GCCTCAATGT GGGACCATCC TCCACACAGC TATATATGGT GCGAACAATG 
CTGGAGTCGC TGACAGCCGA TAAGAGTGGC GGCAAGCGTA CATTGCGCAA GGATATCGAC 
GGGAACTGCC TAATGCAGAT CGAAACGTTC CACAACACGT CGTTCTACTG GAGCTATTTG 
CTCAACTTTA GCGACACTCT ACAGAAATGC TGCGATCTGT CGCAGCTCTG GTATCGCGAG 
TTCTATTTGG AAATGACAAT GGGCCGCAAG GTCAACAAGT GCATGGTGCG TCACCAGCAC 
AACGAAGAGT GTAAGGATTT GATAACAATG GAGAAGCGTA TACAGTTCCC TATTGAGATG 
TCTATGCCGT GGATTCTCAC CGATCATATA CTGCAAACAA AAGAGCCCTC CATGATGGAG 
TTCGTCCTGT ACCCGTTGGA CCTTTACAAC GATTCGGCAC ACTATGCCCT AACCGTCTTC 
CGCAAGCAGT TCCTCTACGA CGAGGTAGAG GCCGAGGTGA ACCTGTGCTT TGATCAGTTT 
GTCTACAAGT TAAGTGAGCA GATCTTTGCT CACTATAAAC AATTGGCTGG CAGCATATTC 
TTGGACAAAC GTTTCCGGCT TGAGTGCGAG GTGTTAGGCT TCAACTTCCA GTCGTATCCA 
CGAAACAATC GCTATGAGAC TCTACTAAAG CAACGACATG TCCAGCTATT GGGCCGATCT 
ATTGACTTGA ACAAACTGGT CACCCAACGC ATCAACGCCA ATATGCACAA GAGCATTGAG 
CTGGCCATCA GCCGCTTTGA GGCCAATGAC ATAACTGGGA TTGTGGAACT CGAGGGCCTT 
TTGGAGGCGA ATCGCATTTG CCACAAATTG CTGAGCAAAT ATTTGGCATT GGACAATTTC 
GATGGCATGG TCAAGGAGGC TAATCACAAT GTCCTGGCCC CCTACGGTCG GATAACGCTG 
CATGTTTTTG TGGAATTGAA TTACGACTTC CTGGTCAATT ACTGCTATAA TGCGGCCACA 
AATCGGTTTA TTCGCACCAA AGTCAATTTG TCGTCGACGC AGGCCATTCA ACGGGAGAAG 
CCGCCACAGA TGTCTCACTA TTATTTGTGG GGCTCCAAGC AGTTGAATGC CGCATACTCT 
ACACAGTATG GGCAGTACAC TGGTTTCGTT GGATCGCCGC ATTTCCATGC CATGTGCCGA 
CTGCTTGGCT ACCAGGGCAT TGCCGTTGTC ATGGACATCA TACTGAAGGA CATTGTCAAG 
CCGTTGATCC AGGGCTCGTT GCTGCAGTTC ACCAAAACGC TTATGATTGC CATGCCCAAA 
TCTTGCAAAT TACCGCGCTG CGAGTACGGA TCGCCCGGCG TGCTCAGCTA TTATCAGGCT 
CATCTTACGG ACATTGTGCA ATACCCGGAC ACGAAAACCG AACTATTTCA GTCTTTCAGG 
GAGTTTGGCA ATTGCATCAT CTTTTGCCTG CTGATTGAGC AGGCTTTGTC CCAGGAGGAG 
GTGTGCGATC TGCTGCACGC GGCCCTCTTC CAAAACATCT TCCCGAGACC CTTCTGCAAA 
GAAAACGAGA AACCTGAAGC CAAACAAAAG CGACTGGAGG CTCAGTTTGC CAACTTGCAG 
ATCGTCTCGA ATGTGGAAAA AATTGGCACT GCCAAGCAAG CTATGATAGC CCGCGAGGGG 
GATTTACTGA CACGCGAGCG CCTCTGTTGC GGCCTGAGCA TATTTGAAGT GATACTCAAT 
CGAGTGAAGA GCTACCTGGA CGATCCAGTG TGGTGTGGCC CGCCACCAGC CAATGGCATA 
ATCCATGTGG ACGAGTGCTC CGAGTTCCAT CGCCTCTGGT CGGCATTGCA GTTTGTCTAT 
TGCATTCCTG TGCGTGGCAC GGAGTACACG ATTGAGGAGC TCTTTGGCGA GGGTCTCAAC 
TGGGCAGGTT GTGTCATGAT CGTGTTGCTG GGTCAGCAGC GACGCTTTGA GGCCCTGGAC 
TTTTGCTATC ACATTTTACG AGTGCAACGC GTCGATGGCA AAGACGAGGA TGTCAAGGGA 
ATTCAACTGA AGCGAATGGT GGATCGTATT CGACGATTCC AAGTATTAAA CTCACAAATC 
TTCTCCATTC TCAACAAATA TCTGAAGGGC GGCGACGGTG AGGGCTCAAA TGTAGAGCAT 
GTGCGCTGCT TTCCACCGCC ACAGCATCCT TCGGTTATAT CCTCGTCGTC ACACTACCAG 
GATCCCCAAA AGCTGCGCCA GAGCATGAAC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001358600.1
CDS	106..3981
Translation	MTEKITLADALSNVEVLDELSLPDEQPCIEAQPCSIIYKANFDTNFEDRNGFVTGIAKYIEEATTHANLNVLLDEGQKHAVMLYTWRCCSRAIPQPKSNEQPNRVEIYEKTVEVLAPEVNKLLNFMYFQRKAIEAFSGEVKRLCHAEKRKDFVSEAYLLTLGKFINMFAVLDELKNMKSSVKNDYSTYRRAAQFLKVMSDSHTLQESQNLSMFLATQNKIRDTVKDTLEKIVGYEDLLSDVVNICVHMFETKMYLTPEEKHMLVKVMGFGLFLMDSDACNINKLDQKKKIRLDRIDRIFKNLEVVPLFGDMQIAPFNYIKRSKHFDSSKWPLSSSNAISPQADLMVHLPQIREDHVKYISELARYTNEVTTTVKENPSDAENRITADLALRGLQLLSEWTSVVTELYSWKLLHPTDHHQNKECPVEAEEYERATRYNYTSEEKFALIEVIAMIKGLQVLMARIETVLCEAIRRNIYSELQDFVQLSLREPLRKAVKNKKDLIRSIIMSVRETSADWQKGYEPTDDPVSKGKKDPDGGFRIHVPRLNVGPSSTQLYMVRTMLESLTADKSGGKRTLRKDIDGNCLMQIETFHNTSFYWSYLLNFSDTLQKCCDLSQLWYREFYLEMTMGRKVNKCMVRHQHNEECKDLITMEKRIQFPIEMSMPWILTDHILQTKEPSMMEFVLYPLDLYNDSAHYALTVFRKQFLYDEVEAEVNLCFDQFVYKLSEQIFAHYKQLAGSIFLDKRFRLECEVLGFNFQSYPRNNRYETLLKQRHVQLLGRSIDLNKLVTQRINANMHKSIELAISRFEANDITGIVELEGLLEANRICHKLLSKYLALDNFDGMVKEANHNVLAPYGRITLHVFVELNYDFLVNYCYNAATNRFIRTKVNLSSTQAIQREKPPQMSHYYLWGSKQLNAAYSTQYGQYTGFVGSPHFHAMCRLLGYQGIAVVMDIILKDIVKPLIQGSLLQFTKTLMIAMPKSCKLPRCEYGSPGVLSYYQAHLTDIVQYPDTKTELFQSFREFGNCIIFCLLIEQALSQEEVCDLLHAALFQNIFPRPFCKENEKPEAKQKRLEAQFANLQIVSNVEKIGTAKQAMIAREGDLLTRERLCCGLSIFEVILNRVKSYLDDPVWCGPPPANGIIHVDECSEFHRLWSALQFVYCIPVRGTEYTIEELFGEGLNWAGCVMIVLLGQQRRFEALDFCYHILRVQRVDGKDEDVKGIQLKRMVDRIRRFQVLNSQIFSILNKYLKGGDGEGSNVEHVRCFPPPQHPSVISSSSHYQDPQKLRQSMNN

Target ORF information:

RefSeq Version	XM_001358563.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18534), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001358563.3

ORF Insert Sequence:

ATGACGGAGA AGATTACGCT TGCCGATGCA CTGTCCAATG TGGAGGTGCT GGACGAGCTG 
TCGCTGCCCG ATGAGCAGCC ATGTATAGAG GCGCAGCCCT GCTCCATTAT CTACAAGGCA 
AACTTCGATA CAAACTTTGA AGATCGCAAT GGATTTGTCA CTGGAATTGC CAAGTACATA 
GAGGAAGCGA CAACGCACGC GAATTTGAAT GTTTTGCTCG ATGAGGGGCA GAAACATGCA 
GTCATGCTTT ACACCTGGCG ATGCTGCTCT CGGGCCATAC CACAGCCCAA GTCGAACGAG 
CAGCCCAATC GTGTGGAGAT CTATGAGAAG ACGGTCGAAG TGCTTGCCCC AGAAGTGAAC 
AAGCTGCTGA ACTTTATGTA TTTCCAACGC AAAGCCATCG AGGCCTTTTC GGGGGAGGTG 
AAGCGGCTGT GCCACGCAGA GAAGCGCAAG GATTTTGTGT CGGAAGCATA CTTGTTGACG 
CTGGGAAAAT TCATCAACAT GTTTGCTGTT CTTGACGAGC TGAAGAACAT GAAGTCGAGT 
GTAAAAAACG ATTATTCCAC CTATAGGCGG GCAGCTCAAT TCCTGAAGGT GATGTCCGAC 
TCGCACACAC TGCAGGAGTC GCAGAATCTC TCCATGTTCC TGGCCACGCA GAACAAAATA 
CGCGACACTG TCAAGGACAC GCTGGAAAAG ATCGTCGGCT ACGAGGATCT GCTCTCGGAT 
GTCGTTAATA TCTGTGTGCA CATGTTCGAG ACGAAAATGT ACTTGACGCC CGAGGAGAAG 
CATATGCTGG TGAAGGTAAT GGGCTTTGGC CTCTTCCTCA TGGACAGTGA TGCCTGCAAC 
ATCAACAAGT TGGACCAAAA GAAGAAAATC CGCCTGGACC GCATCGATCG CATCTTTAAG 
AACCTGGAGG TGGTGCCCCT CTTCGGTGAT ATGCAGATAG CACCCTTCAA CTATATAAAG 
CGGAGCAAGC ACTTCGACTC CAGCAAGTGG CCGCTCTCCA GCTCGAATGC CATCAGCCCT 
CAGGCTGATT TGATGGTGCA CTTGCCGCAG ATACGGGAGG ATCATGTCAA GTACATATCA 
GAGCTGGCGC GATACACCAA CGAGGTCACC ACCACTGTCA AGGAGAATCC ATCGGATGCT 
GAGAATCGGA TTACGGCCGA CTTGGCCCTG CGCGGTCTGC AGCTTCTGTC GGAGTGGACA 
AGTGTGGTCA CTGAGCTGTA CTCCTGGAAG CTGCTCCATC CCACAGACCA CCACCAGAAC 
AAGGAGTGTC CCGTGGAGGC CGAGGAGTAC GAAAGGGCAA CCCGATACAA TTACACTTCC 
GAGGAGAAGT TTGCCCTGAT TGAAGTGATC GCCATGATCA AAGGCCTGCA GGTGCTGATG 
GCACGCATCG AGACGGTGCT GTGTGAGGCT ATTCGACGCA ATATTTATTC CGAGCTGCAG 
GACTTTGTTC AGCTATCGCT GCGCGAACCC CTTCGGAAGG CGGTGAAGAA CAAAAAGGAT 
CTAATCCGGA GCATCATTAT GTCGGTGCGG GAGACATCTG CGGATTGGCA AAAGGGCTAC 
GAGCCCACCG ACGATCCCGT GTCCAAAGGC AAAAAAGATC CCGATGGCGG CTTTCGTATC 
CATGTGCCAC GCCTCAATGT GGGACCATCC TCCACACAGC TATATATGGT GCGAACAATG 
CTGGAGTCGC TGACAGCCGA TAAGAGTGGC GGCAAGCGTA CATTGCGCAA GGATATCGAC 
GGGAACTGCC TAATGCAGAT CGAAACGTTC CACAACACGT CGTTCTACTG GAGCTATTTG 
CTCAACTTTA GCGACACTCT ACAGAAATGC TGCGATCTGT CGCAGCTCTG GTATCGCGAG 
TTCTATTTGG AAATGACAAT GGGCCGCAAG GTCAACAAGT GCATGGTGCG TCACCAGCAC 
AACGAAGAGT GTAAGGATTT GATAACAATG GAGAAGCGTA TACAGTTCCC TATTGAGATG 
TCTATGCCGT GGATTCTCAC CGATCATATA CTGCAAACAA AAGAGCCCTC CATGATGGAG 
TTCGTCCTGT ACCCGTTGGA CCTTTACAAC GATTCGGCAC ACTATGCCCT AACCGTCTTC 
CGCAAGCAGT TCCTCTACGA CGAGGTAGAG GCCGAGGTGA ACCTGTGCTT TGATCAGTTT 
GTCTACAAGT TAAGTGAGCA GATCTTTGCT CACTATAAAC AATTGGCTGG CAGCATATTC 
TTGGACAAAC GTTTCCGGCT TGAGTGCGAG GTGTTAGGCT TCAACTTCCA GTCGTATCCA 
CGAAACAATC GCTATGAGAC TCTACTAAAG CAACGACATG TCCAGCTATT GGGCCGATCT 
ATTGACTTGA ACAAACTGGT CACCCAACGC ATCAACGCCA ATATGCACAA GAGCATTGAG 
CTGGCCATCA GCCGCTTTGA GGCCAATGAC ATAACTGGGA TTGTGGAACT CGAGGGCCTT 
TTGGAGGCGA ATCGCATTTG CCACAAATTG CTGAGCAAAT ATTTGGCATT GGACAATTTC 
GATGGCATGG TCAAGGAGGC TAATCACAAT GTCCTGGCCC CCTACGGTCG GATAACGCTG 
CATGTTTTTG TGGAATTGAA TTACGACTTC CTGGTCAATT ACTGCTATAA TGCGGCCACA 
AATCGGTTTA TTCGCACCAA AGTCAATTTG TCGTCGACGC AGGCCATTCA ACGGGAGAAG 
CCGCCACAGA TGTCTCACTA TTATTTGTGG GGCTCCAAGC AGTTGAATGC CGCATACTCT 
ACACAGTATG GGCAGTACAC TGGTTTCGTT GGATCGCCGC ATTTCCATGC CATGTGCCGA 
CTGCTTGGCT ACCAGGGCAT TGCCGTTGTC ATGGACATCA TACTGAAGGA CATTGTCAAG 
CCGTTGATCC AGGGCTCGTT GCTGCAGTTC ACCAAAACGC TTATGATTGC CATGCCCAAA 
TCTTGCAAAT TACCGCGCTG CGAGTACGGA TCGCCCGGCG TGCTCAGCTA TTATCAGGCT 
CATCTTACGG ACATTGTGCA ATACCCGGAC ACGAAAACCG AACTATTTCA GTCTTTCAGG 
GAGTTTGGCA ATTGCATCAT CTTTTGCCTG CTGATTGAGC AGGCTTTGTC CCAGGAGGAG 
GTGTGCGATC TGCTGCACGC GGCCCTCTTC CAAAACATCT TCCCGAGACC CTTCTGCAAA 
GAAAACGAGA AACCTGAAGC CAAACAAAAG CGACTGGAGG CTCAGTTTGC CAACTTGCAG 
ATCGTCTCGA ATGTGGAAAA AATTGGCACT GCCAAGCAAG CTATGATAGC CCGCGAGGGG 
GATTTACTGA CACGCGAGCG CCTCTGTTGC GGCCTGAGCA TATTTGAAGT GATACTCAAT 
CGAGTGAAGA GCTACCTGGA CGATCCAGTG TGGTGTGGCC CGCCACCAGC CAATGGCATA 
ATCCATGTGG ACGAGTGCTC CGAGTTCCAT CGCCTCTGGT CGGCATTGCA GTTTGTCTAT 
TGCATTCCTG TGCGTGGCAC GGAGTACACG ATTGAGGAGC TCTTTGGCGA GGGTCTCAAC 
TGGGCAGGTT GTGTCATGAT CGTGTTGCTG GGTCAGCAGC GACGCTTTGA GGCCCTGGAC 
TTTTGCTATC ACATTTTACG AGTGCAACGC GTCGATGGCA AAGACGAGGA TGTCAAGGGA 
ATTCAACTGA AGCGAATGGT GGATCGTATT CGACGATTCC AAGTATTAAA CTCACAAATC 
TTCTCCATTC TCAACAAATA TCTGAAGGGC GGCGACGGTG AGGGCTCAAA TGTAGAGCAT 
GTGCGCTGCT TTCCACCGCC ACAGCATCCT TCGGTTATAT CCTCGTCGTC ACACTACCAG 
GATCCCCAAA AGCTGCGCCA GAGCATGAAC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA18534 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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