SFI1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SFI1
Entrez Gene ID	560147
Full Name	SFI1 centrin binding protein
General protein information	Preferred Names SFI1 centrin binding protein Names protein SFI1 homolog Sfi1 homolog, spindle assembly associated spindle assembly associated Sfi1 homolog
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_683537.8	XP_688629.5	protein SFI1 homolog
XM_683537.7	XP_688629.5	protein SFI1 homolog

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sfi1	NP_084483.2
Danio rerio (zebrafish)	sfi1	XP_688629.5
Pan troglodytes (chimpanzee)	SFI1	XP_001148452.1
Macaca mulatta (Rhesus monkey)	LOC716833	XP_001111056.1
Canis lupus familiaris (dog)	SFI1	XP_005636542.1
Bos taurus (cattle)	SFI1	XP_005218239.1
Rattus norvegicus (Norway rat)	Sfi1	NP_001178611.1
Homo sapiens (human)	SFI1	NP_001007468.1
Gallus gallus (chicken)	SFI1	XP_004934420.1
Xenopus tropicalis (tropical clawed frog)	sfi1	XP_004910651.1

Gene Ontology

The following SFI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12138	XM_683537.8 Latest version!	Danio rerio SFI1 centrin binding protein (sfi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa12138	XM_683537.7	Danio rerio SFI1 centrin binding protein (sfi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SFI1 ( XM_683537.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_688629.5 Danio rerio SFI1 centrin binding protein (sfi1), mRNA.

Price & Availability↑

CloneID	ODa12138
Clone ID Related Accession (Same CDS sequence)	XM_683537.8 , XM_683537.7
Accession Version	XM_683537.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein SFI1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_683537.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACGA CAAGACCACA CGCTGGGAAA CAGCTGTCGT GTGCTGTTGA AAAGAACCGG 
GCACGGAAGA CAGTAACACG CAAAAACACT TACCGGGTCG GATATACTTG GAACCGAGGT 
GGTAGACTTA AAGAGCTGAG GATACGGCAC CTTGCTCGAA AGTTCCTTTT CTTGTGGATT 
AGGAGGACAT TTGGACGAAA AACAACATCA CAAGCCAGAT CTCATTATTG TAAGGTCATC 
CTGAAGAAGG TCTTCAGGTC TTGGAAAGAT GAATGGTGGC ATTCAAGAAA GGAATGGACT 
CTCAATATAA GGGCCGACTG CCATTATGCG TATACAGTGT ACAGCAAAAC ATTTCAAGCC 
TTGCAGGAAT ATGTTGCTGT CCAGAGGGAA GAAAAGAAGA AACTACAGCT TGCGATCACT 
TTTGAGACGG AGCACAAGCT GCGCTGTGTG TGGAATAGCT GGGAGCTTTA CCTGGACATG 
TGTCGCATGA AACGCAGGAT GCAAGAGGCA GCAGTACAGC ACAAGAATCT CACTGTGCAA 
AAATGGGTTT GGACTGTATG GAACTTGGCA CTACAGCACA GAAAATTGGA GTACCACCAA 
CAAGATCTTG GCTTGCAACA TTGGGCACAC AGTGTACAGA GCAGAGCATG GCTGCACTGG 
AGGGACCGGA TGAAACATGC CTGTGCTCTA AAAGAGAAAG AGTCCAAAGC TCATCATCAC 
TATTGTCTTC AACTGTTAAA GCAAACGCTG CATGGTTGGA TCAGACATGC AGAAAACAGA 
CAGGCTAAGA ACAAGAAAAT AGCTGTTGCT CAGGATGTGC GGCGTCTCTC TGTGCTGGAG 
AGGTTTTGGT GGCAGTGGTT TGAAGTCCTG CAATCCAGAC GTGTTGAGAA AGACAGAGGA 
CAGAGAGCAG ACAAACTGGC CCAGCACGGC TCCCAGCGGC TGGTCTTCTC CCACTGGAGA 
CACTATGTCA GTATATGCTC ACAGAAGGCT AAGAGGGAAA AGAGTGCCAT GCATCACAGA 
CAACGTTATC TTCTGCATTT GGGACTCAAA GGCTTTGCGC TAAATGTCAC TCAAAGTAAG 
ACTTATCGTC TCAACAAGAA CATTAGTGTT CAGCATCGTC ATCAGAATTT GCTGGCAATG 
TGCTGGAATG TCTGGCAGTT GCGTTTGGAT CGGGAAGAGA ACAGACGTCT GCAGCCCCAG 
ATGAGTGTTG CACAAAACCA TCACAAATTT TCTGTGCTGA GATTTAACCT TCATCATTGG 
AAAAAACGTC TTGCAGAGCA CAAAAAATGT CAGGAACTGG AACTACGTGC TGATGCCTGC 
TTTGCCCAGC GTATATTCCC ACAATGTGTA AACACTTGGA TGGAGTTTAC AGCACAACGC 
ACAGAGAAAC GAGAGCAGAA AGAGCGAGCT GAACAGCAGT ATAGGCAGCA GACCTTTAGT 
TGGGTATTTT ATACTTGGTG GAGAAACCTG GAGGCAAGGA GGGATCGGAG ACTTGCTGAA 
AGAACTGCGG TGTTGCATGC TGAGCATGTG TGTTATGTTC GTGTCTGGTC TAAGTGGTAC 
AGTGGTGCTG TTCAGCGCAG AGAGGAGAGA ATTAAACAGG CTGTAGCCGA CTCACTGTAC 
AAACACACTC TGCAACAGAA GAACCTGAAC CACTGGAAAA ATCGTTTCAT AAACATACAG 
ACCAGTCAGA AGCGTTTTAA GCAGGCAGAG GGTCATCATG AACAGAGATG TCTGAAAAGA 
GCCATGACAG ACTGGCATCA GTATGTTGAT CACAGAAGAC TGAAGAAAGA GAAGATTGCA 
GAAATGGAGA AACATTACCA CAACAAATTG CTCAAACATG CTCTTGATGC TTGGAAGTCA 
TACCATTTGC AGACACAGGC AATCAGCCAA CTGGTAGAGT ACAGTTACCA GGAGCATGAG 
CAGCATCTGG TCAGGAGAAT GTTCTGTTTA TGGAGGATAA ACGTGTTACA GTTGGTGGAA 
GAGAGAGAAA AGGAAAACAG AGCAACATGT TTGTCTCAAA AGCACCTTGT ATCCCAGGTG 
TTTCTTGCAT GGCAACAGAG AACTGTATCT GCACACCTTC ACCGTCACAA AAAGAACGAA 
GCCCTCAAAC AAGCCCAAAC ACAACTGGAG AAATTACGCA TACAAGTGGC TTTGAGAAAA 
TGGAAAGAGC GAAGCATTGA AGTGAAGGAT GAGAGATTGG CAAGCGAGAG GGCCGACAGA 
CATTACAGCA GAACACTGAG GGAGAAAACT CTCAGCAATT GGACCAGCTT TATTCACCAT 
CATAAAATAT ATCAGGTGAT GAAGAAAAGA AGCTTTGAAC TTCACAAATT AAGGACTTGT 
CAGCACTTTT TTATCTGCTG GAAGACACAG CTGCAGAGCA GAAGACGAGA GGCGGAGCAA 
ACTGAGACTG CTCTTTGGCA TTGGTCCCTC AACCTACAGG CCAAGGTGTT CTGCATGTGG 
CGGATCTGGA TTGCAGAGCG TCAGAGGAAA CAGAAGCGTT TGACTGAGGC AGCTCAGTTT 
TATAGAGATG AACTGTTGCG AGAGGGCGTC ACACACATCC TTACACACAC GGCACATATG 
AATGCTTTCT CCACTAATAT TGCCCAGCAC AGCTATGAAC AGAGCTGTCG TCAGCTTCAG 
GAGGTGGTGA GGCGATGCGC TCTTCGATGG AAGCAGCGTG CGCTATGCAA ACCTGTCAAG 
GATAAATCTA CACCGTCTAA AGACAGTCAA GCCAAGAAAA GTGTGTCTTT TTTTCTGCCA 
GGAGACCCCA TGCCCACCCT CTGTCCAGGC AGGATCCAAT CACCAGAGCA GAGGCAGAAA 
GATTCTGTCA TTCATCAGAT GTGGTCAGTT CGAGCATCTA GACCACAACC AAGGAGACCA 
GGTGACCTTC TGGAGTCCCC AGCCAAACAT CTGTCACGTG ACCTTAAGAT CAGCAGGGGT 
CAGAGGGTGT TTGTTTCAGA GACTGCATTT CCATCAGTCC CTTCTACCTT TCCTTCACCA 
CTGCCCTCAG TGCCTTTGAC CCACAACCCT CTGAATTCAC CTGCAATGGT GCTACAGCCT 
AAAACGCTCC TCCTCAAACC TGACTCTCCA GGATGTATCG CCACTACCAG CCAGGACATA 
CTGCTTCCTC CATCCACATT CACTCTCTCA ACAGCGCATA GCAAGCAAAA ATACTTGAGA 
GATGGTGGGC CACTACTGCA TTCCAGTCAT TTCACTTCCC GATTTTTCCC AACACACGGT 
TTACATGAGA GAATACCAGA GGATGAAGAA GAGGATGTGG ATGTGGAGCA AACTGAAAAT 
CTCACCAAAG AACTACTAGA TATTAGACTG GAGATGCAAC GATACCAGCA AGACAGAAAA 
CAGCTACAGA CATGGCGCAA GCTTCAAAAG GTCTTGGGTA ACTGGCTTGA AACGACAGGC 
ACTGAGGGGG AAACTGATGA AAGAGACAGC ATCTTAAAGG AGTTAACTGA GCTAGAGAGT 
CGGATTTCTT CTCTATCTAT GAGAATAAAA AAGCAGAAAC CCTCCATGAT TCGCCATGCT 
GCCAGAGTTA ACACTATCCA GAGCCAACTA CTACCATCTG AGGGCACCAC AAACCTACCT 
ACTGAGTATG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688629.5
CDS	769..4386
Translation	MQTTRPHAGKQLSCAVEKNRARKTVTRKNTYRVGYTWNRGGRLKELRIRHLARKFLFLWIRRTFGRKTTSQARSHYCKVILKKVFRSWKDEWWHSRKEWTLNIRADCHYAYTVYSKTFQALQEYVAVQREEKKKLQLAITFETEHKLRCVWNSWELYLDMCRMKRRMQEAAVQHKNLTVQKWVWTVWNLALQHRKLEYHQQDLGLQHWAHSVQSRAWLHWRDRMKHACALKEKESKAHHHYCLQLLKQTLHGWIRHAENRQAKNKKIAVAQDVRRLSVLERFWWQWFEVLQSRRVEKDRGQRADKLAQHGSQRLVFSHWRHYVSICSQKAKREKSAMHHRQRYLLHLGLKGFALNVTQSKTYRLNKNISVQHRHQNLLAMCWNVWQLRLDREENRRLQPQMSVAQNHHKFSVLRFNLHHWKKRLAEHKKCQELELRADACFAQRIFPQCVNTWMEFTAQRTEKREQKERAEQQYRQQTFSWVFYTWWRNLEARRDRRLAERTAVLHAEHVCYVRVWSKWYSGAVQRREERIKQAVADSLYKHTLQQKNLNHWKNRFINIQTSQKRFKQAEGHHEQRCLKRAMTDWHQYVDHRRLKKEKIAEMEKHYHNKLLKHALDAWKSYHLQTQAISQLVEYSYQEHEQHLVRRMFCLWRINVLQLVEEREKENRATCLSQKHLVSQVFLAWQQRTVSAHLHRHKKNEALKQAQTQLEKLRIQVALRKWKERSIEVKDERLASERADRHYSRTLREKTLSNWTSFIHHHKIYQVMKKRSFELHKLRTCQHFFICWKTQLQSRRREAEQTETALWHWSLNLQAKVFCMWRIWIAERQRKQKRLTEAAQFYRDELLREGVTHILTHTAHMNAFSTNIAQHSYEQSCRQLQEVVRRCALRWKQRALCKPVKDKSTPSKDSQAKKSVSFFLPGDPMPTLCPGRIQSPEQRQKDSVIHQMWSVRASRPQPRRPGDLLESPAKHLSRDLKISRGQRVFVSETAFPSVPSTFPSPLPSVPLTHNPLNSPAMVLQPKTLLLKPDSPGCIATTSQDILLPPSTFTLSTAHSKQKYLRDGGPLLHSSHFTSRFFPTHGLHERIPEDEEEDVDVEQTENLTKELLDIRLEMQRYQQDRKQLQTWRKLQKVLGNWLETTGTEGETDERDSILKELTELESRISSLSMRIKKQKPSMIRHAARVNTIQSQLLPSEGTTNLPTEYGI

Target ORF information:

RefSeq Version	XM_683537.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SFI1 centrin binding protein (sfi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683537.8

ORF Insert Sequence:

ATGCAAACGA CAAGACCACA CGCTGGGAAA CAGCTGTCGT GTGCTGTTGA AAAGAACCGG 
GCACGGAAGA CAGTAACACG CAAAAACACT TACCGGGTCG GATATACTTG GAACCGAGGT 
GGTAGACTTA AAGAGCTGAG GATACGGCAC CTTGCTCGAA AGTTCCTTTT CTTGTGGATT 
AGGAGGACAT TTGGACGAAA AACAACATCA CAAGCCAGAT CTCATTATTG TAAGGTCATC 
CTGAAGAAGG TCTTCAGGTC TTGGAAAGAT GAATGGTGGC ATTCAAGAAA GGAATGGACT 
CTCAATATAA GGGCCGACTG CCATTATGCG TATACAGTGT ACAGCAAAAC ATTTCAAGCC 
TTGCAGGAAT ATGTTGCTGT CCAGAGGGAA GAAAAGAAGA AACTACAGCT TGCGATCACT 
TTTGAGACGG AGCACAAGCT GCGCTGTGTG TGGAATAGCT GGGAGCTTTA CCTGGACATG 
TGTCGCATGA AACGCAGGAT GCAAGAGGCA GCAGTACAGC ACAAGAATCT CACTGTGCAA 
AAATGGGTTT GGACTGTATG GAACTTGGCA CTACAGCACA GAAAATTGGA GTACCACCAA 
CAAGATCTTG GCTTGCAACA TTGGGCACAC AGTGTACAGA GCAGAGCATG GCTGCACTGG 
AGGGACCGGA TGAAACATGC CTGTGCTCTA AAAGAGAAAG AGTCCAAAGC TCATCATCAC 
TATTGTCTTC AACTGTTAAA GCAAACGCTG CATGGTTGGA TCAGACATGC AGAAAACAGA 
CAGGCTAAGA ACAAGAAAAT AGCTGTTGCT CAGGATGTGC GGCGTCTCTC TGTGCTGGAG 
AGGTTTTGGT GGCAGTGGTT TGAAGTCCTG CAATCCAGAC GTGTTGAGAA AGACAGAGGA 
CAGAGAGCAG ACAAACTGGC CCAGCACGGC TCCCAGCGGC TGGTCTTCTC CCACTGGAGA 
CACTATGTCA GTATATGCTC ACAGAAGGCT AAGAGGGAAA AGAGTGCCAT GCATCACAGA 
CAACGTTATC TTCTGCATTT GGGACTCAAA GGCTTTGCGC TAAATGTCAC TCAAAGTAAG 
ACTTATCGTC TCAACAAGAA CATTAGTGTT CAGCATCGTC ATCAGAATTT GCTGGCAATG 
TGCTGGAATG TCTGGCAGTT GCGTTTGGAT CGGGAAGAGA ACAGACGTCT GCAGCCCCAG 
ATGAGTGTTG CACAAAACCA TCACAAATTT TCTGTGCTGA GATTTAACCT TCATCATTGG 
AAAAAACGTC TTGCAGAGCA CAAAAAATGT CAGGAACTGG AACTACGTGC TGATGCCTGC 
TTTGCCCAGC GTATATTCCC ACAATGTGTA AACACTTGGA TGGAGTTTAC AGCACAACGC 
ACAGAGAAAC GAGAGCAGAA AGAGCGAGCT GAACAGCAGT ATAGGCAGCA GACCTTTAGT 
TGGGTATTTT ATACTTGGTG GAGAAACCTG GAGGCAAGGA GGGATCGGAG ACTTGCTGAA 
AGAACTGCGG TGTTGCATGC TGAGCATGTG TGTTATGTTC GTGTCTGGTC TAAGTGGTAC 
AGTGGTGCTG TTCAGCGCAG AGAGGAGAGA ATTAAACAGG CTGTAGCCGA CTCACTGTAC 
AAACACACTC TGCAACAGAA GAACCTGAAC CACTGGAAAA ATCGTTTCAT AAACATACAG 
ACCAGTCAGA AGCGTTTTAA GCAGGCAGAG GGTCATCATG AACAGAGATG TCTGAAAAGA 
GCCATGACAG ACTGGCATCA GTATGTTGAT CACAGAAGAC TGAAGAAAGA GAAGATTGCA 
GAAATGGAGA AACATTACCA CAACAAATTG CTCAAACATG CTCTTGATGC TTGGAAGTCA 
TACCATTTGC AGACACAGGC AATCAGCCAA CTGGTAGAGT ACAGTTACCA GGAGCATGAG 
CAGCATCTGG TCAGGAGAAT GTTCTGTTTA TGGAGGATAA ACGTGTTACA GTTGGTGGAA 
GAGAGAGAAA AGGAAAACAG AGCAACATGT TTGTCTCAAA AGCACCTTGT ATCCCAGGTG 
TTTCTTGCAT GGCAACAGAG AACTGTATCT GCACACCTTC ACCGTCACAA AAAGAACGAA 
GCCCTCAAAC AAGCCCAAAC ACAACTGGAG AAATTACGCA TACAAGTGGC TTTGAGAAAA 
TGGAAAGAGC GAAGCATTGA AGTGAAGGAT GAGAGATTGG CAAGCGAGAG GGCCGACAGA 
CATTACAGCA GAACACTGAG GGAGAAAACT CTCAGCAATT GGACCAGCTT TATTCACCAT 
CATAAAATAT ATCAGGTGAT GAAGAAAAGA AGCTTTGAAC TTCACAAATT AAGGACTTGT 
CAGCACTTTT TTATCTGCTG GAAGACACAG CTGCAGAGCA GAAGACGAGA GGCGGAGCAA 
ACTGAGACTG CTCTTTGGCA TTGGTCCCTC AACCTACAGG CCAAGGTGTT CTGCATGTGG 
CGGATCTGGA TTGCAGAGCG TCAGAGGAAA CAGAAGCGTT TGACTGAGGC AGCTCAGTTT 
TATAGAGATG AACTGTTGCG AGAGGGCGTC ACACACATCC TTACACACAC GGCACATATG 
AATGCTTTCT CCACTAATAT TGCCCAGCAC AGCTATGAAC AGAGCTGTCG TCAGCTTCAG 
GAGGTGGTGA GGCGATGCGC TCTTCGATGG AAGCAGCGTG CGCTATGCAA ACCTGTCAAG 
GATAAATCTA CACCGTCTAA AGACAGTCAA GCCAAGAAAA GTGTGTCTTT TTTTCTGCCA 
GGAGACCCCA TGCCCACCCT CTGTCCAGGC AGGATCCAAT CACCAGAGCA GAGGCAGAAA 
GATTCTGTCA TTCATCAGAT GTGGTCAGTT CGAGCATCTA GACCACAACC AAGGAGACCA 
GGTGACCTTC TGGAGTCCCC AGCCAAACAT CTGTCACGTG ACCTTAAGAT CAGCAGGGGT 
CAGAGGGTGT TTGTTTCAGA GACTGCATTT CCATCAGTCC CTTCTACCTT TCCTTCACCA 
CTGCCCTCAG TGCCTTTGAC CCACAACCCT CTGAATTCAC CTGCAATGGT GCTACAGCCT 
AAAACGCTCC TCCTCAAACC TGACTCTCCA GGATGTATCG CCACTACCAG CCAGGACATA 
CTGCTTCCTC CATCCACATT CACTCTCTCA ACAGCGCATA GCAAGCAAAA ATACTTGAGA 
GATGGTGGGC CACTACTGCA TTCCAGTCAT TTCACTTCCC GATTTTTCCC AACACACGGT 
TTACATGAGA GAATACCAGA GGATGAAGAA GAGGATGTGG ATGTGGAGCA AACTGAAAAT 
CTCACCAAAG AACTACTAGA TATTAGACTG GAGATGCAAC GATACCAGCA AGACAGAAAA 
CAGCTACAGA CATGGCGCAA GCTTCAAAAG GTCTTGGGTA ACTGGCTTGA AACGACAGGC 
ACTGAGGGGG AAACTGATGA AAGAGACAGC ATCTTAAAGG AGTTAACTGA GCTAGAGAGT 
CGGATTTCTT CTCTATCTAT GAGAATAAAA AAGCAGAAAC CCTCCATGAT TCGCCATGCT 
GCCAGAGTTA ACACTATCCA GAGCCAACTA CTACCATCTG AGGGCACCAC AAACCTACCT 
ACTGAGTATG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SFI1 ( XM_683537.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_688629.5 Danio rerio SFI1 centrin binding protein (sfi1), mRNA.

Price & Availability↑

CloneID	ODa12138
Clone ID Related Accession (Same CDS sequence)	XM_683537.8 , XM_683537.7
Accession Version	XM_683537.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein SFI1 homolog
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_683537.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACGA CAAGACCACA CGCTGGGAAA CAGCTGTCGT GTGCTGTTGA AAAGAACCGG 
GCACGGAAGA CAGTAACACG CAAAAACACT TACCGGGTCG GATATACTTG GAACCGAGGT 
GGTAGACTTA AAGAGCTGAG GATACGGCAC CTTGCTCGAA AGTTCCTTTT CTTGTGGATT 
AGGAGGACAT TTGGACGAAA AACAACATCA CAAGCCAGAT CTCATTATTG TAAGGTCATC 
CTGAAGAAGG TCTTCAGGTC TTGGAAAGAT GAATGGTGGC ATTCAAGAAA GGAATGGACT 
CTCAATATAA GGGCCGACTG CCATTATGCG TATACAGTGT ACAGCAAAAC ATTTCAAGCC 
TTGCAGGAAT ATGTTGCTGT CCAGAGGGAA GAAAAGAAGA AACTACAGCT TGCGATCACT 
TTTGAGACGG AGCACAAGCT GCGCTGTGTG TGGAATAGCT GGGAGCTTTA CCTGGACATG 
TGTCGCATGA AACGCAGGAT GCAAGAGGCA GCAGTACAGC ACAAGAATCT CACTGTGCAA 
AAATGGGTTT GGACTGTATG GAACTTGGCA CTACAGCACA GAAAATTGGA GTACCACCAA 
CAAGATCTTG GCTTGCAACA TTGGGCACAC AGTGTACAGA GCAGAGCATG GCTGCACTGG 
AGGGACCGGA TGAAACATGC CTGTGCTCTA AAAGAGAAAG AGTCCAAAGC TCATCATCAC 
TATTGTCTTC AACTGTTAAA GCAAACGCTG CATGGTTGGA TCAGACATGC AGAAAACAGA 
CAGGCTAAGA ACAAGAAAAT AGCTGTTGCT CAGGATGTGC GGCGTCTCTC TGTGCTGGAG 
AGGTTTTGGT GGCAGTGGTT TGAAGTCCTG CAATCCAGAC GTGTTGAGAA AGACAGAGGA 
CAGAGAGCAG ACAAACTGGC CCAGCACGGC TCCCAGCGGC TGGTCTTCTC CCACTGGAGA 
CACTATGTCA GTATATGCTC ACAGAAGGCT AAGAGGGAAA AGAGTGCCAT GCATCACAGA 
CAACGTTATC TTCTGCATTT GGGACTCAAA GGCTTTGCGC TAAATGTCAC TCAAAGTAAG 
ACTTATCGTC TCAACAAGAA CATTAGTGTT CAGCATCGTC ATCAGAATTT GCTGGCAATG 
TGCTGGAATG TCTGGCAGTT GCGTTTGGAT CGGGAAGAGA ACAGACGTCT GCAGCCCCAG 
ATGAGTGTTG CACAAAACCA TCACAAATTT TCTGTGCTGA GATTTAACCT TCATCATTGG 
AAAAAACGTC TTGCAGAGCA CAAAAAATGT CAGGAACTGG AACTACGTGC TGATGCCTGC 
TTTGCCCAGC GTATATTCCC ACAATGTGTA AACACTTGGA TGGAGTTTAC AGCACAACGC 
ACAGAGAAAC GAGAGCAGAA AGAGCGAGCT GAACAGCAGT ATAGGCAGCA GACCTTTAGT 
TGGGTATTTT ATACTTGGTG GAGAAACCTG GAGGCAAGGA GGGATCGGAG ACTTGCTGAA 
AGAACTGCGG TGTTGCATGC TGAGCATGTG TGTTATGTTC GTGTCTGGTC TAAGTGGTAC 
AGTGGTGCTG TTCAGCGCAG AGAGGAGAGA ATTAAACAGG CTGTAGCCGA CTCACTGTAC 
AAACACACTC TGCAACAGAA GAACCTGAAC CACTGGAAAA ATCGTTTCAT AAACATACAG 
ACCAGTCAGA AGCGTTTTAA GCAGGCAGAG GGTCATCATG AACAGAGATG TCTGAAAAGA 
GCCATGACAG ACTGGCATCA GTATGTTGAT CACAGAAGAC TGAAGAAAGA GAAGATTGCA 
GAAATGGAGA AACATTACCA CAACAAATTG CTCAAACATG CTCTTGATGC TTGGAAGTCA 
TACCATTTGC AGACACAGGC AATCAGCCAA CTGGTAGAGT ACAGTTACCA GGAGCATGAG 
CAGCATCTGG TCAGGAGAAT GTTCTGTTTA TGGAGGATAA ACGTGTTACA GTTGGTGGAA 
GAGAGAGAAA AGGAAAACAG AGCAACATGT TTGTCTCAAA AGCACCTTGT ATCCCAGGTG 
TTTCTTGCAT GGCAACAGAG AACTGTATCT GCACACCTTC ACCGTCACAA AAAGAACGAA 
GCCCTCAAAC AAGCCCAAAC ACAACTGGAG AAATTACGCA TACAAGTGGC TTTGAGAAAA 
TGGAAAGAGC GAAGCATTGA AGTGAAGGAT GAGAGATTGG CAAGCGAGAG GGCCGACAGA 
CATTACAGCA GAACACTGAG GGAGAAAACT CTCAGCAATT GGACCAGCTT TATTCACCAT 
CATAAAATAT ATCAGGTGAT GAAGAAAAGA AGCTTTGAAC TTCACAAATT AAGGACTTGT 
CAGCACTTTT TTATCTGCTG GAAGACACAG CTGCAGAGCA GAAGACGAGA GGCGGAGCAA 
ACTGAGACTG CTCTTTGGCA TTGGTCCCTC AACCTACAGG CCAAGGTGTT CTGCATGTGG 
CGGATCTGGA TTGCAGAGCG TCAGAGGAAA CAGAAGCGTT TGACTGAGGC AGCTCAGTTT 
TATAGAGATG AACTGTTGCG AGAGGGCGTC ACACACATCC TTACACACAC GGCACATATG 
AATGCTTTCT CCACTAATAT TGCCCAGCAC AGCTATGAAC AGAGCTGTCG TCAGCTTCAG 
GAGGTGGTGA GGCGATGCGC TCTTCGATGG AAGCAGCGTG CGCTATGCAA ACCTGTCAAG 
GATAAATCTA CACCGTCTAA AGACAGTCAA GCCAAGAAAA GTGTGTCTTT TTTTCTGCCA 
GGAGACCCCA TGCCCACCCT CTGTCCAGGC AGGATCCAAT CACCAGAGCA GAGGCAGAAA 
GATTCTGTCA TTCATCAGAT GTGGTCAGTT CGAGCATCTA GACCACAACC AAGGAGACCA 
GGTGACCTTC TGGAGTCCCC AGCCAAACAT CTGTCACGTG ACCTTAAGAT CAGCAGGGGT 
CAGAGGGTGT TTGTTTCAGA GACTGCATTT CCATCAGTCC CTTCTACCTT TCCTTCACCA 
CTGCCCTCAG TGCCTTTGAC CCACAACCCT CTGAATTCAC CTGCAATGGT GCTACAGCCT 
AAAACGCTCC TCCTCAAACC TGACTCTCCA GGATGTATCG CCACTACCAG CCAGGACATA 
CTGCTTCCTC CATCCACATT CACTCTCTCA ACAGCGCATA GCAAGCAAAA ATACTTGAGA 
GATGGTGGGC CACTACTGCA TTCCAGTCAT TTCACTTCCC GATTTTTCCC AACACACGGT 
TTACATGAGA GAATACCAGA GGATGAAGAA GAGGATGTGG ATGTGGAGCA AACTGAAAAT 
CTCACCAAAG AACTACTAGA TATTAGACTG GAGATGCAAC GATACCAGCA AGACAGAAAA 
CAGCTACAGA CATGGCGCAA GCTTCAAAAG GTCTTGGGTA ACTGGCTTGA AACGACAGGC 
ACTGAGGGGG AAACTGATGA AAGAGACAGC ATCTTAAAGG AGTTAACTGA GCTAGAGAGT 
CGGATTTCTT CTCTATCTAT GAGAATAAAA AAGCAGAAAC CCTCCATGAT TCGCCATGCT 
GCCAGAGTTA ACACTATCCA GAGCCAACTA CTACCATCTG AGGGCACCAC AAACCTACCT 
ACTGAGTATG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688629.5
CDS	654..4271
Translation	MQTTRPHAGKQLSCAVEKNRARKTVTRKNTYRVGYTWNRGGRLKELRIRHLARKFLFLWIRRTFGRKTTSQARSHYCKVILKKVFRSWKDEWWHSRKEWTLNIRADCHYAYTVYSKTFQALQEYVAVQREEKKKLQLAITFETEHKLRCVWNSWELYLDMCRMKRRMQEAAVQHKNLTVQKWVWTVWNLALQHRKLEYHQQDLGLQHWAHSVQSRAWLHWRDRMKHACALKEKESKAHHHYCLQLLKQTLHGWIRHAENRQAKNKKIAVAQDVRRLSVLERFWWQWFEVLQSRRVEKDRGQRADKLAQHGSQRLVFSHWRHYVSICSQKAKREKSAMHHRQRYLLHLGLKGFALNVTQSKTYRLNKNISVQHRHQNLLAMCWNVWQLRLDREENRRLQPQMSVAQNHHKFSVLRFNLHHWKKRLAEHKKCQELELRADACFAQRIFPQCVNTWMEFTAQRTEKREQKERAEQQYRQQTFSWVFYTWWRNLEARRDRRLAERTAVLHAEHVCYVRVWSKWYSGAVQRREERIKQAVADSLYKHTLQQKNLNHWKNRFINIQTSQKRFKQAEGHHEQRCLKRAMTDWHQYVDHRRLKKEKIAEMEKHYHNKLLKHALDAWKSYHLQTQAISQLVEYSYQEHEQHLVRRMFCLWRINVLQLVEEREKENRATCLSQKHLVSQVFLAWQQRTVSAHLHRHKKNEALKQAQTQLEKLRIQVALRKWKERSIEVKDERLASERADRHYSRTLREKTLSNWTSFIHHHKIYQVMKKRSFELHKLRTCQHFFICWKTQLQSRRREAEQTETALWHWSLNLQAKVFCMWRIWIAERQRKQKRLTEAAQFYRDELLREGVTHILTHTAHMNAFSTNIAQHSYEQSCRQLQEVVRRCALRWKQRALCKPVKDKSTPSKDSQAKKSVSFFLPGDPMPTLCPGRIQSPEQRQKDSVIHQMWSVRASRPQPRRPGDLLESPAKHLSRDLKISRGQRVFVSETAFPSVPSTFPSPLPSVPLTHNPLNSPAMVLQPKTLLLKPDSPGCIATTSQDILLPPSTFTLSTAHSKQKYLRDGGPLLHSSHFTSRFFPTHGLHERIPEDEEEDVDVEQTENLTKELLDIRLEMQRYQQDRKQLQTWRKLQKVLGNWLETTGTEGETDERDSILKELTELESRISSLSMRIKKQKPSMIRHAARVNTIQSQLLPSEGTTNLPTEYGI

Target ORF information:

RefSeq Version	XM_683537.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SFI1 centrin binding protein (sfi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683537.7

ORF Insert Sequence:

ATGCAAACGA CAAGACCACA CGCTGGGAAA CAGCTGTCGT GTGCTGTTGA AAAGAACCGG 
GCACGGAAGA CAGTAACACG CAAAAACACT TACCGGGTCG GATATACTTG GAACCGAGGT 
GGTAGACTTA AAGAGCTGAG GATACGGCAC CTTGCTCGAA AGTTCCTTTT CTTGTGGATT 
AGGAGGACAT TTGGACGAAA AACAACATCA CAAGCCAGAT CTCATTATTG TAAGGTCATC 
CTGAAGAAGG TCTTCAGGTC TTGGAAAGAT GAATGGTGGC ATTCAAGAAA GGAATGGACT 
CTCAATATAA GGGCCGACTG CCATTATGCG TATACAGTGT ACAGCAAAAC ATTTCAAGCC 
TTGCAGGAAT ATGTTGCTGT CCAGAGGGAA GAAAAGAAGA AACTACAGCT TGCGATCACT 
TTTGAGACGG AGCACAAGCT GCGCTGTGTG TGGAATAGCT GGGAGCTTTA CCTGGACATG 
TGTCGCATGA AACGCAGGAT GCAAGAGGCA GCAGTACAGC ACAAGAATCT CACTGTGCAA 
AAATGGGTTT GGACTGTATG GAACTTGGCA CTACAGCACA GAAAATTGGA GTACCACCAA 
CAAGATCTTG GCTTGCAACA TTGGGCACAC AGTGTACAGA GCAGAGCATG GCTGCACTGG 
AGGGACCGGA TGAAACATGC CTGTGCTCTA AAAGAGAAAG AGTCCAAAGC TCATCATCAC 
TATTGTCTTC AACTGTTAAA GCAAACGCTG CATGGTTGGA TCAGACATGC AGAAAACAGA 
CAGGCTAAGA ACAAGAAAAT AGCTGTTGCT CAGGATGTGC GGCGTCTCTC TGTGCTGGAG 
AGGTTTTGGT GGCAGTGGTT TGAAGTCCTG CAATCCAGAC GTGTTGAGAA AGACAGAGGA 
CAGAGAGCAG ACAAACTGGC CCAGCACGGC TCCCAGCGGC TGGTCTTCTC CCACTGGAGA 
CACTATGTCA GTATATGCTC ACAGAAGGCT AAGAGGGAAA AGAGTGCCAT GCATCACAGA 
CAACGTTATC TTCTGCATTT GGGACTCAAA GGCTTTGCGC TAAATGTCAC TCAAAGTAAG 
ACTTATCGTC TCAACAAGAA CATTAGTGTT CAGCATCGTC ATCAGAATTT GCTGGCAATG 
TGCTGGAATG TCTGGCAGTT GCGTTTGGAT CGGGAAGAGA ACAGACGTCT GCAGCCCCAG 
ATGAGTGTTG CACAAAACCA TCACAAATTT TCTGTGCTGA GATTTAACCT TCATCATTGG 
AAAAAACGTC TTGCAGAGCA CAAAAAATGT CAGGAACTGG AACTACGTGC TGATGCCTGC 
TTTGCCCAGC GTATATTCCC ACAATGTGTA AACACTTGGA TGGAGTTTAC AGCACAACGC 
ACAGAGAAAC GAGAGCAGAA AGAGCGAGCT GAACAGCAGT ATAGGCAGCA GACCTTTAGT 
TGGGTATTTT ATACTTGGTG GAGAAACCTG GAGGCAAGGA GGGATCGGAG ACTTGCTGAA 
AGAACTGCGG TGTTGCATGC TGAGCATGTG TGTTATGTTC GTGTCTGGTC TAAGTGGTAC 
AGTGGTGCTG TTCAGCGCAG AGAGGAGAGA ATTAAACAGG CTGTAGCCGA CTCACTGTAC 
AAACACACTC TGCAACAGAA GAACCTGAAC CACTGGAAAA ATCGTTTCAT AAACATACAG 
ACCAGTCAGA AGCGTTTTAA GCAGGCAGAG GGTCATCATG AACAGAGATG TCTGAAAAGA 
GCCATGACAG ACTGGCATCA GTATGTTGAT CACAGAAGAC TGAAGAAAGA GAAGATTGCA 
GAAATGGAGA AACATTACCA CAACAAATTG CTCAAACATG CTCTTGATGC TTGGAAGTCA 
TACCATTTGC AGACACAGGC AATCAGCCAA CTGGTAGAGT ACAGTTACCA GGAGCATGAG 
CAGCATCTGG TCAGGAGAAT GTTCTGTTTA TGGAGGATAA ACGTGTTACA GTTGGTGGAA 
GAGAGAGAAA AGGAAAACAG AGCAACATGT TTGTCTCAAA AGCACCTTGT ATCCCAGGTG 
TTTCTTGCAT GGCAACAGAG AACTGTATCT GCACACCTTC ACCGTCACAA AAAGAACGAA 
GCCCTCAAAC AAGCCCAAAC ACAACTGGAG AAATTACGCA TACAAGTGGC TTTGAGAAAA 
TGGAAAGAGC GAAGCATTGA AGTGAAGGAT GAGAGATTGG CAAGCGAGAG GGCCGACAGA 
CATTACAGCA GAACACTGAG GGAGAAAACT CTCAGCAATT GGACCAGCTT TATTCACCAT 
CATAAAATAT ATCAGGTGAT GAAGAAAAGA AGCTTTGAAC TTCACAAATT AAGGACTTGT 
CAGCACTTTT TTATCTGCTG GAAGACACAG CTGCAGAGCA GAAGACGAGA GGCGGAGCAA 
ACTGAGACTG CTCTTTGGCA TTGGTCCCTC AACCTACAGG CCAAGGTGTT CTGCATGTGG 
CGGATCTGGA TTGCAGAGCG TCAGAGGAAA CAGAAGCGTT TGACTGAGGC AGCTCAGTTT 
TATAGAGATG AACTGTTGCG AGAGGGCGTC ACACACATCC TTACACACAC GGCACATATG 
AATGCTTTCT CCACTAATAT TGCCCAGCAC AGCTATGAAC AGAGCTGTCG TCAGCTTCAG 
GAGGTGGTGA GGCGATGCGC TCTTCGATGG AAGCAGCGTG CGCTATGCAA ACCTGTCAAG 
GATAAATCTA CACCGTCTAA AGACAGTCAA GCCAAGAAAA GTGTGTCTTT TTTTCTGCCA 
GGAGACCCCA TGCCCACCCT CTGTCCAGGC AGGATCCAAT CACCAGAGCA GAGGCAGAAA 
GATTCTGTCA TTCATCAGAT GTGGTCAGTT CGAGCATCTA GACCACAACC AAGGAGACCA 
GGTGACCTTC TGGAGTCCCC AGCCAAACAT CTGTCACGTG ACCTTAAGAT CAGCAGGGGT 
CAGAGGGTGT TTGTTTCAGA GACTGCATTT CCATCAGTCC CTTCTACCTT TCCTTCACCA 
CTGCCCTCAG TGCCTTTGAC CCACAACCCT CTGAATTCAC CTGCAATGGT GCTACAGCCT 
AAAACGCTCC TCCTCAAACC TGACTCTCCA GGATGTATCG CCACTACCAG CCAGGACATA 
CTGCTTCCTC CATCCACATT CACTCTCTCA ACAGCGCATA GCAAGCAAAA ATACTTGAGA 
GATGGTGGGC CACTACTGCA TTCCAGTCAT TTCACTTCCC GATTTTTCCC AACACACGGT 
TTACATGAGA GAATACCAGA GGATGAAGAA GAGGATGTGG ATGTGGAGCA AACTGAAAAT 
CTCACCAAAG AACTACTAGA TATTAGACTG GAGATGCAAC GATACCAGCA AGACAGAAAA 
CAGCTACAGA CATGGCGCAA GCTTCAAAAG GTCTTGGGTA ACTGGCTTGA AACGACAGGC 
ACTGAGGGGG AAACTGATGA AAGAGACAGC ATCTTAAAGG AGTTAACTGA GCTAGAGAGT 
CGGATTTCTT CTCTATCTAT GAGAATAAAA AAGCAGAAAC CCTCCATGAT TCGCCATGCT 
GCCAGAGTTA ACACTATCCA GAGCCAACTA CTACCATCTG AGGGCACCAC AAACCTACCT 
ACTGAGTATG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SFI1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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