CNE03250 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNE03250
Entrez Gene ID	3257777
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024657244.1	XP_024512987.1	conserved hypothetical protein
XM_571204.1	XP_571204.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Loftus BJ, Fung E, Roncaglia P, Rowley D, Amedeo P, Bruno D, Vamathevan J, Miranda M, Anderson IJ, Fraser JA, Allen JE, Bosdet IE, Brent MR, Chiu R, Doering TL, Donlin MJ, D'Souza CA, Fox DS, Grinberg V, Fu J, Fukushima M, Haas BJ, Huang JC, Janbon G, Jones SJ, Koo HL, Krzywinski MI, Kwon-Chung JK, Lengeler KB, Maiti R, Marra MA, Marra RE, Mathewson CA, Mitchell TG, Pertea M, Riggs FR, Salzberg SL, Schein JE, Shvartsbeyn A, Shin H, Shumway M, Specht CA, Suh BB, Tenney A, Utterback TR, Wickes BL, Wortman JR, Wye NH, Kronstad JW, Lodge JK, Heitman J, Davis RW, Fraser CM, Hyman RW
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNE03250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNE03250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCn04477	XM_571204.1 Latest version!	Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNE03250), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCn08263	XM_024657244.1 Latest version!	Cryptococcus neoformans var. neoformans JEC21 conserved hypothetical protein (CNE03250), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNE03250 ( XM_571204.1 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_571204.1 Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNE03250), partial mRNA.

Price & Availability↑

CloneID	OCn04477
Clone ID Related Accession (Same CDS sequence)	XM_571204.1
Accession Version	XM_571204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1345046400000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006687). The reference sequence was derived from mrna.CNE03250.1. This record represents the complete annotated sequence of the C. neoformans JEC21 genome. A small number of gaps exist which are represented by NNNNs within the final sequence. The genome has been annotated by TIGR and the annotation data is available for viewing and download from the TIGR database (http://www.tigr.org/tdb/e2k1/cna1/). COMPLETENESS: incomplete on both ends.

ATGCCCAAAG CAAACAAATC CTCGGCTTCA GCGGGGACTC GTAAGAAGCA TGCCGCCAAG 
AAGACTCACA GAGAGCACGA TGACGATTCT GATCATCAAC CAGAGTCGGG AACACCTCAA 
CCTTCGAGAA AACAGCGAGG GGAGAAAAAG CTCTCCAAGG CGCAAAAGAG AGCGATGCCG 
AAAGTGAAGC AATACATCCC TCCACCAAAA CCCCCTGCAC CTCCCATTCT GGATCCTCTC 
GACGGACAGG GCCTTGCGAG AACTTTGCCG GCGGATTTGG TCGTCGTACT GAGACGATTA 
GGAAAGAAGG ACGCTGTTAC AAGGCGCAAA GGTCTTGAGG AATTTAGGGA TGGCTGGGTG 
AAATACATTC TGAAGGAGAA ACCAACTGAA GAGGAAGAAA CTGAACGAGA AGTGAAGGAG 
ATTGCACTTC TAGCAGCTAT ACCTGTCTGG TCCCCTTTCC ACAGAGCTAT TGCTCTCCTT 
ATTCATACAG ATCTTCTTTC TGTGCCTGCC CTTCGGGCTT CAATTTTGGA AAGCCTAAAT 
TTGGGCCTGT TACCTGGTAC CCAAAACCGT GATGTTCTTG GGTCTTGGAT GATAGCTGCT 
TTAGAAGAAG GTCGAAGGGC AGGTGGACTA GGGCTCAGGG GATGGGAATC GTCTATCCTC 
TGGAAGGCGA GCGGTGAAGA GGTTGGCGGT GATACAGACA TTCGCATTGA TCTCAAAGAA 
CACCTTTCCA CTTTAATCGA GTACCTTACT CTATCTACCC TGAACCCTTC GATCCTACAC 
GAGGACATCC ATCCAATACC TGTAGCAGCT ACCCCGGTGT CTTCTATTCC TTTGGGCAAA 
AAAGGTACTA ACAAGCAAGG GAAATCAAAA ACATCTTCTG CCCCACCGTC TCGACAGACT 
GAAACACCTG TACCACCTCC GGAGGATCAT GAAATTCTTG AAGAAAGGAT GACAAGGTAT 
CGAGTGGGCG GACTTATAGG TCTTTCATGG CTCCTGCAAC AACTACCTCA AGTGGGATTG 
ACATCCTTGC CTGAAGATTT GGTCACTTTG CTACGTCATC CTGCATTATG GCTTTCCTTG 
TCTTCAGAAC AGGTTGATAC AGTAAATTCA CCTGGGTCCT TGGGCACTGC TCAGCCTCCT 
GTTAGAAAGG CAGGTTATGG GCTCCTCGAT GTGCTTGTAA ACTCTTACGA AAGTATTGTG 
GCTGAGCCCG AGATGCTCAA AATGGTGTCA AACGCTGTCT TGAGTAGCTG TTGGAGAGAA 
AAAGAGGCAG TGGTATGGGA ATCTGCAGGG TCCGCTATTG CCAAGTTTAT CCGCAAGTCT 
GCTGCAGACC ATCGTGATGG GCGCAATGAT GGGCGCAATG ATGGGGAAGA GGAGGTCGAC 
AGTGAAGACG AGAATGTTCA AAACGCCGAT GACGAAGATG GCGCGAATCC GGCCACTCTC 
CTTCAGCTTT CTTCACCATA CTACTCTGAT TTCTTGGACT TCACATCCAC TATTTGCCCT 
TATATTCCTC ATATCACTTA CCCCATAATT CTTGTTGTTC TCTCCACCCT TCCGCCCTCC 
TTATTGCTGC TTGTTGGGCC ATCCCTTCAG CTCCAGACGT TATTCTCTCA TCTTTGGGCC 
CCCCTGGATT CTCGCTTACT TTCCACGCAT TGTCTGCCAG GTCAAAAGTC TGCATTCCAA 
GTGTTTTTCC AGGCTATATT GGAGTGTACC ATATTCCTCA TTGGCAAGTC ATGGAAAGAG 
AAGGACGGAA AAGAGGCTGC AATGTGGTTG GTGAAGGAGC AGCTAGGCAA CAGAGCATGG 
AAAGAGGGCG TTCTGCTTAT GGGCGGAAGG GCTGGTGGAA GGCATGTCCC TCGAGGATGT 
ACCGAAGAGA GTGAAGCTTC TGCATTTGGC AGCACTCTTG GCCGTCTGGC ATCTATTGAT 
CAACTACTCT TAGACCAAAT CCGGGTCGTG CTTACCCAGA CGCTATCTGA TGCTTGTTTC 
CCGCAAGAGA ACCCGGAGAG CGGTCCTATG GAGGTAAGTA TTTTACCGAG AAGCTTGAGT 
ATACTCTCTG CTATAAGAGA CGCGATTGCT GCTAGTCAAG TGATCGGCCT AGTGGATCAA 
TTAATGGAGG ATTTGATCGA AAAATTATCT GAAGTGTTTG TGGGCTCTTC AAAGAGCGAC 
AATCCCTCCC ATGCAATCAT GGCGGAGACA TTAGTAGATG CTCTGAAGAA CCATCACCAT 
TTGGTGCACA GCGACACAAT AGAGAAACTT TTGGGCCAAG TCAAAGATTC GTCCCACGAG 
CTACTCATAA TCCTGCACCC ACCTTTGTTC ATTGCTTATC TAGACAGCCT CTGCTCCAAG 
TTGCCGTCTG CCGAGCGACA TGTTTGCCAA CAAGCTCTTT CTACCGTGCT GCAGTCTTCC 
GAAATAGACT CTTCGCAGCG CTTCACAGTT GCCAATGCTG TGTTGATTCT TGACCATACC 
CAACTTCTAC AATCGGGCTC ACTGGATCAA ATTGTTAAAG AAGCCACGCA TGACGCTCTA 
GCAGGCGGAT CACCTATTGC CATCCCCGTG GTCACCGCAT CTCTCAAATC CCTTTGCTAC 
CTATCAAAAT CTACCCTGGA TGAGGTGTTA TCTGTTACCT GCAGCGTCAT TCGTGAATCA 
ACCAAAAAGC TTCTGACCAC CGATTTCGTT GAGCTTGGTG TTCCGCAGGC GGCCTTCGAG 
ATCTTTGCTG CCTATGCCAC AAACCATCTT CAGGAAGTCA TCGAATCGGA GCATTTGATT 
TCGGGTCTGG TCGGCGTTCA TCATGTCCTA TTCGTTTTAC CTAGATTACC TGGTCACATT 
GCCTCAGCCG AGTCAAAGGC TAGCGGCCTG CTCAGTTTAT GGTCCAAGCT GGGTGAACTG 
GTTGTGGAAT ACCAGGAGAA GGTCCTCCAA AGAGTGTACA GTGCCTTAAA AGGAGAAATG 
GGTGATGTAG AAGTCAGGGT GGACCCTGAG GTGCTCATCG ACGTGGCTCT TGCAACAACC 
CTCGGGTCCC ACTCTGCTCC TTCCCTCCAA GCGCTTGTCC TGGCGTTGCT TCCCGAAGTG 
ATCAGTATCA TTCAGGATCT CGCTCCTCAT ACATCCCAAC CTCCCCATCC GTCTCTGCCT 
ATCGTGGACC CACTTATACC GTTTGTCCCC CATGGTGACG ATGACCTTGA CTCTATTCAA 
CCAGAGTTTG ATAGCCTTGG TCGAGCTCAG ACAGCCAGAT ACGCCGAGGC GGCGGTAGCT 
CTACTCCGAG CAGACAGAAG CCTTGTTAAG GATGAACCTG CTTTATTGCA AATGATTTTA 
GCTGTCAGTC GACTAGCACA GGATGCTCTG ACTGTTCCCG GAGCATCCAG AGGGCTGTTT 
TCGAAAGAGA CACCTTCCAC CCGCCTAGCT GATCTGGTTC GAGAAGTAGA AGGGGCCGTT 
TCTTTCGCTC TTGGATATGT GGACGAGGTT GAGCCCTCTT GGCACCATGC CACCATTCAT 
GCTCTCAAAT CTGGATCTGT GCCACATGAT TCCGACTTGT TACAAAGCTT AATGGTTGCG 
CTGCAGAAAG AAGTGACTGC AAATGGTAAC GACGTGGATG CCAGGTCATT TAGGGATGTG 
CTTTACAAGC ACATGAGACA AATAGGAGCG GGTGTCCAAG AAGGGGAGGC ATGGCTAAAC 
TATGCCATGA GTCTGGTTGA CAGATCACCA CAGCTCAGTC TCGCCATAAT AACTGCCATC 
AAACCTCTCA TGCTTGACAC ACAGGCTTTT GCCACTGCCC AAACCCGTCT CGCATCGGCT 
CTTACAACCA TTCCCCCGTC CAAGTGTCAT ACTGCACTGA ATCTGTTGAG GATCTTCAAC 
GCCTCAGCTC CAGAGGTCGA TTCTACTTCT GTATTTCTCC CTCAGCAGCG AGCCCTCTTT 
GTACTTCGCC ACGTCGCTGG ATGGCTCACA AGTGATGATG TCGACGAGGA CAAGTTGCCT 
GAGGATATGG AGTTTAGAGT CTTGGAGCTC GAAGGGTCAG TGGCGCCAAT CGTGCAAGAC 
GTGGGTGGCG CACACTGGGA TGGGATCTTT GACTTGGTTG AAAACGGCTT AGAGAATTCG 
GGAATGGACG ACTCCTCTTC GCTCTGTTTG CTTTACCATA GCTTGTCCGT CCTTCAGCAG 
ATCAGGGATC TCTGTCAGAC CAACAAGTCT TTGAGAGCTT CTTGGACAAG TAAAACGAAT 
CATATGAAAC TTGTCTTGGA TCTATTCTTG CAATGTCGCT CAGCGAACTC TGTCCCTCTA 
CAATTAATTC AAAATCTTAT TCTTGACCTC CTGCCTGACG TCCCCGAGCA GGTCATGAAA 
GAGGCCAGCT TGACATCACT TTGTAATCTG ATATCCCTTT CGACATCTTC GGCTATCCAA 
ACTGCTGCTT ATCGAATCAT TTCTCAAGTT ATCAGACATC AAACGGTTGG ACTGGTCTTG 
GAAGTAGAAG CATCTGTAGC GCAAGCCCAA GAAGGCCATG CCGAAAGAAC CATTGAGATG 
GCAAAGGAGT TGGTGGATGT TGTTAAAATT GGAAGTGAAG TAAATTGGCA TGGCGAGCTT 
GGTGTATCCC TAGTCTTGGG ACAACTATTA GCTTGGATGT CTATATTGGA TTATTTCGAG 
GATGCTTCTC GGACTTTACG CTGGGCGTAC CTGGATCAAC TAAATACATC CAAGCTGATC 
ACGGACGGCC TCGTTCCAAT GCTCTTCTCC ATCCTTGGAG TATCTGAAAT GGGGGCCTGG 
AACTTCCCCG CCAGCCAATA CGCTGTAGAT GAGTTCTTCA CGGAGTTCCT TGACCCGGAG 
TCCTTGCCGG ATCTTTCCCC ACTTGCCTCT CATCTATTCT ACCGAGCACT GGTCACTATT 
CCATCTTCTG TCCGAGCATA TTACGAATCT CTTAAGGATC GCCAGCTATC TATGTCCATG 
CTCTCATTCA CTGCTCGGCA CTACTCTCCG GTAATCATTG CGCACGAGTT CTCTGCTTTA 
AGGGAACCGT CGGCGATGGC TCAGCTAACT GAAGAAGGCT TGAATGTGCG GATAGCGCAA 
GGTGGTGGTG CAACTATCGC AGCAGGAGCT GGGTCCGCTG AGGCAATTGC CAGCTACGTG 
GTCGATGAAC AGCCAATGGA AATTGGCATC AGATTACCGG CAGAGTTCCC GCTGAAAGGA 
GTCGATGTCA GAGATCTGCG AAGGGTGGGG GTGCCAGAGA ACAAGTGGCG AGGGTGGTTG 
ATGGGTTTAC AGCAAACTAT CACATCAAGG AATGGGTTGA TTCTCGAGGC ACTTACAATG 
TTTAAGAAAA ATGTTTCGCT GCATTTTGAG GGTGTCGTGG AATGTGCTAT CTGCTATTCT 
ATCATATCCC TTACGGACAG GACGCTTCCC ACCAAGCCAT GCCGAACGTG CAAGAATCGA 
TTCCATGCGA GCTGTCTCTT CAAATGGTTC AATTCGTCTC ATTCGAGCAG CTGTCCTCTT 
TGTCGAGCTC TGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_571204.1
CDS	1..5478
Translation	MPKANKSSASAGTRKKHAAKKTHREHDDDSDHQPESGTPQPSRKQRGEKKLSKAQKRAMPKVKQYIPPPKPPAPPILDPLDGQGLARTLPADLVVVLRRLGKKDAVTRRKGLEEFRDGWVKYILKEKPTEEEETEREVKEIALLAAIPVWSPFHRAIALLIHTDLLSVPALRASILESLNLGLLPGTQNRDVLGSWMIAALEEGRRAGGLGLRGWESSILWKASGEEVGGDTDIRIDLKEHLSTLIEYLTLSTLNPSILHEDIHPIPVAATPVSSIPLGKKGTNKQGKSKTSSAPPSRQTETPVPPPEDHEILEERMTRYRVGGLIGLSWLLQQLPQVGLTSLPEDLVTLLRHPALWLSLSSEQVDTVNSPGSLGTAQPPVRKAGYGLLDVLVNSYESIVAEPEMLKMVSNAVLSSCWREKEAVVWESAGSAIAKFIRKSAADHRDGRNDGRNDGEEEVDSEDENVQNADDEDGANPATLLQLSSPYYSDFLDFTSTICPYIPHITYPIILVVLSTLPPSLLLLVGPSLQLQTLFSHLWAPLDSRLLSTHCLPGQKSAFQVFFQAILECTIFLIGKSWKEKDGKEAAMWLVKEQLGNRAWKEGVLLMGGRAGGRHVPRGCTEESEASAFGSTLGRLASIDQLLLDQIRVVLTQTLSDACFPQENPESGPMEVSILPRSLSILSAIRDAIAASQVIGLVDQLMEDLIEKLSEVFVGSSKSDNPSHAIMAETLVDALKNHHHLVHSDTIEKLLGQVKDSSHELLIILHPPLFIAYLDSLCSKLPSAERHVCQQALSTVLQSSEIDSSQRFTVANAVLILDHTQLLQSGSLDQIVKEATHDALAGGSPIAIPVVTASLKSLCYLSKSTLDEVLSVTCSVIRESTKKLLTTDFVELGVPQAAFEIFAAYATNHLQEVIESEHLISGLVGVHHVLFVLPRLPGHIASAESKASGLLSLWSKLGELVVEYQEKVLQRVYSALKGEMGDVEVRVDPEVLIDVALATTLGSHSAPSLQALVLALLPEVISIIQDLAPHTSQPPHPSLPIVDPLIPFVPHGDDDLDSIQPEFDSLGRAQTARYAEAAVALLRADRSLVKDEPALLQMILAVSRLAQDALTVPGASRGLFSKETPSTRLADLVREVEGAVSFALGYVDEVEPSWHHATIHALKSGSVPHDSDLLQSLMVALQKEVTANGNDVDARSFRDVLYKHMRQIGAGVQEGEAWLNYAMSLVDRSPQLSLAIITAIKPLMLDTQAFATAQTRLASALTTIPPSKCHTALNLLRIFNASAPEVDSTSVFLPQQRALFVLRHVAGWLTSDDVDEDKLPEDMEFRVLELEGSVAPIVQDVGGAHWDGIFDLVENGLENSGMDDSSSLCLLYHSLSVLQQIRDLCQTNKSLRASWTSKTNHMKLVLDLFLQCRSANSVPLQLIQNLILDLLPDVPEQVMKEASLTSLCNLISLSTSSAIQTAAYRIISQVIRHQTVGLVLEVEASVAQAQEGHAERTIEMAKELVDVVKIGSEVNWHGELGVSLVLGQLLAWMSILDYFEDASRTLRWAYLDQLNTSKLITDGLVPMLFSILGVSEMGAWNFPASQYAVDEFFTEFLDPESLPDLSPLASHLFYRALVTIPSSVRAYYESLKDRQLSMSMLSFTARHYSPVIIAHEFSALREPSAMAQLTEEGLNVRIAQGGGATIAAGAGSAEAIASYVVDEQPMEIGIRLPAEFPLKGVDVRDLRRVGVPENKWRGWLMGLQQTITSRNGLILEALTMFKKNVSLHFEGVVECAICYSIISLTDRTLPTKPCRTCKNRFHASCLFKWFNSSHSSSCPLCRALF

Target ORF information:

RefSeq Version	XM_571204.1
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNE03250), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_571204.1

ORF Insert Sequence:

ATGCCCAAAG CAAACAAATC CTCGGCTTCA GCGGGGACTC GTAAGAAGCA TGCCGCCAAG 
AAGACTCACA GAGAGCACGA TGACGATTCT GATCATCAAC CAGAGTCGGG AACACCTCAA 
CCTTCGAGAA AACAGCGAGG GGAGAAAAAG CTCTCCAAGG CGCAAAAGAG AGCGATGCCG 
AAAGTGAAGC AATACATCCC TCCACCAAAA CCCCCTGCAC CTCCCATTCT GGATCCTCTC 
GACGGACAGG GCCTTGCGAG AACTTTGCCG GCGGATTTGG TCGTCGTACT GAGACGATTA 
GGAAAGAAGG ACGCTGTTAC AAGGCGCAAA GGTCTTGAGG AATTTAGGGA TGGCTGGGTG 
AAATACATTC TGAAGGAGAA ACCAACTGAA GAGGAAGAAA CTGAACGAGA AGTGAAGGAG 
ATTGCACTTC TAGCAGCTAT ACCTGTCTGG TCCCCTTTCC ACAGAGCTAT TGCTCTCCTT 
ATTCATACAG ATCTTCTTTC TGTGCCTGCC CTTCGGGCTT CAATTTTGGA AAGCCTAAAT 
TTGGGCCTGT TACCTGGTAC CCAAAACCGT GATGTTCTTG GGTCTTGGAT GATAGCTGCT 
TTAGAAGAAG GTCGAAGGGC AGGTGGACTA GGGCTCAGGG GATGGGAATC GTCTATCCTC 
TGGAAGGCGA GCGGTGAAGA GGTTGGCGGT GATACAGACA TTCGCATTGA TCTCAAAGAA 
CACCTTTCCA CTTTAATCGA GTACCTTACT CTATCTACCC TGAACCCTTC GATCCTACAC 
GAGGACATCC ATCCAATACC TGTAGCAGCT ACCCCGGTGT CTTCTATTCC TTTGGGCAAA 
AAAGGTACTA ACAAGCAAGG GAAATCAAAA ACATCTTCTG CCCCACCGTC TCGACAGACT 
GAAACACCTG TACCACCTCC GGAGGATCAT GAAATTCTTG AAGAAAGGAT GACAAGGTAT 
CGAGTGGGCG GACTTATAGG TCTTTCATGG CTCCTGCAAC AACTACCTCA AGTGGGATTG 
ACATCCTTGC CTGAAGATTT GGTCACTTTG CTACGTCATC CTGCATTATG GCTTTCCTTG 
TCTTCAGAAC AGGTTGATAC AGTAAATTCA CCTGGGTCCT TGGGCACTGC TCAGCCTCCT 
GTTAGAAAGG CAGGTTATGG GCTCCTCGAT GTGCTTGTAA ACTCTTACGA AAGTATTGTG 
GCTGAGCCCG AGATGCTCAA AATGGTGTCA AACGCTGTCT TGAGTAGCTG TTGGAGAGAA 
AAAGAGGCAG TGGTATGGGA ATCTGCAGGG TCCGCTATTG CCAAGTTTAT CCGCAAGTCT 
GCTGCAGACC ATCGTGATGG GCGCAATGAT GGGCGCAATG ATGGGGAAGA GGAGGTCGAC 
AGTGAAGACG AGAATGTTCA AAACGCCGAT GACGAAGATG GCGCGAATCC GGCCACTCTC 
CTTCAGCTTT CTTCACCATA CTACTCTGAT TTCTTGGACT TCACATCCAC TATTTGCCCT 
TATATTCCTC ATATCACTTA CCCCATAATT CTTGTTGTTC TCTCCACCCT TCCGCCCTCC 
TTATTGCTGC TTGTTGGGCC ATCCCTTCAG CTCCAGACGT TATTCTCTCA TCTTTGGGCC 
CCCCTGGATT CTCGCTTACT TTCCACGCAT TGTCTGCCAG GTCAAAAGTC TGCATTCCAA 
GTGTTTTTCC AGGCTATATT GGAGTGTACC ATATTCCTCA TTGGCAAGTC ATGGAAAGAG 
AAGGACGGAA AAGAGGCTGC AATGTGGTTG GTGAAGGAGC AGCTAGGCAA CAGAGCATGG 
AAAGAGGGCG TTCTGCTTAT GGGCGGAAGG GCTGGTGGAA GGCATGTCCC TCGAGGATGT 
ACCGAAGAGA GTGAAGCTTC TGCATTTGGC AGCACTCTTG GCCGTCTGGC ATCTATTGAT 
CAACTACTCT TAGACCAAAT CCGGGTCGTG CTTACCCAGA CGCTATCTGA TGCTTGTTTC 
CCGCAAGAGA ACCCGGAGAG CGGTCCTATG GAGGTAAGTA TTTTACCGAG AAGCTTGAGT 
ATACTCTCTG CTATAAGAGA CGCGATTGCT GCTAGTCAAG TGATCGGCCT AGTGGATCAA 
TTAATGGAGG ATTTGATCGA AAAATTATCT GAAGTGTTTG TGGGCTCTTC AAAGAGCGAC 
AATCCCTCCC ATGCAATCAT GGCGGAGACA TTAGTAGATG CTCTGAAGAA CCATCACCAT 
TTGGTGCACA GCGACACAAT AGAGAAACTT TTGGGCCAAG TCAAAGATTC GTCCCACGAG 
CTACTCATAA TCCTGCACCC ACCTTTGTTC ATTGCTTATC TAGACAGCCT CTGCTCCAAG 
TTGCCGTCTG CCGAGCGACA TGTTTGCCAA CAAGCTCTTT CTACCGTGCT GCAGTCTTCC 
GAAATAGACT CTTCGCAGCG CTTCACAGTT GCCAATGCTG TGTTGATTCT TGACCATACC 
CAACTTCTAC AATCGGGCTC ACTGGATCAA ATTGTTAAAG AAGCCACGCA TGACGCTCTA 
GCAGGCGGAT CACCTATTGC CATCCCCGTG GTCACCGCAT CTCTCAAATC CCTTTGCTAC 
CTATCAAAAT CTACCCTGGA TGAGGTGTTA TCTGTTACCT GCAGCGTCAT TCGTGAATCA 
ACCAAAAAGC TTCTGACCAC CGATTTCGTT GAGCTTGGTG TTCCGCAGGC GGCCTTCGAG 
ATCTTTGCTG CCTATGCCAC AAACCATCTT CAGGAAGTCA TCGAATCGGA GCATTTGATT 
TCGGGTCTGG TCGGCGTTCA TCATGTCCTA TTCGTTTTAC CTAGATTACC TGGTCACATT 
GCCTCAGCCG AGTCAAAGGC TAGCGGCCTG CTCAGTTTAT GGTCCAAGCT GGGTGAACTG 
GTTGTGGAAT ACCAGGAGAA GGTCCTCCAA AGAGTGTACA GTGCCTTAAA AGGAGAAATG 
GGTGATGTAG AAGTCAGGGT GGACCCTGAG GTGCTCATCG ACGTGGCTCT TGCAACAACC 
CTCGGGTCCC ACTCTGCTCC TTCCCTCCAA GCGCTTGTCC TGGCGTTGCT TCCCGAAGTG 
ATCAGTATCA TTCAGGATCT CGCTCCTCAT ACATCCCAAC CTCCCCATCC GTCTCTGCCT 
ATCGTGGACC CACTTATACC GTTTGTCCCC CATGGTGACG ATGACCTTGA CTCTATTCAA 
CCAGAGTTTG ATAGCCTTGG TCGAGCTCAG ACAGCCAGAT ACGCCGAGGC GGCGGTAGCT 
CTACTCCGAG CAGACAGAAG CCTTGTTAAG GATGAACCTG CTTTATTGCA AATGATTTTA 
GCTGTCAGTC GACTAGCACA GGATGCTCTG ACTGTTCCCG GAGCATCCAG AGGGCTGTTT 
TCGAAAGAGA CACCTTCCAC CCGCCTAGCT GATCTGGTTC GAGAAGTAGA AGGGGCCGTT 
TCTTTCGCTC TTGGATATGT GGACGAGGTT GAGCCCTCTT GGCACCATGC CACCATTCAT 
GCTCTCAAAT CTGGATCTGT GCCACATGAT TCCGACTTGT TACAAAGCTT AATGGTTGCG 
CTGCAGAAAG AAGTGACTGC AAATGGTAAC GACGTGGATG CCAGGTCATT TAGGGATGTG 
CTTTACAAGC ACATGAGACA AATAGGAGCG GGTGTCCAAG AAGGGGAGGC ATGGCTAAAC 
TATGCCATGA GTCTGGTTGA CAGATCACCA CAGCTCAGTC TCGCCATAAT AACTGCCATC 
AAACCTCTCA TGCTTGACAC ACAGGCTTTT GCCACTGCCC AAACCCGTCT CGCATCGGCT 
CTTACAACCA TTCCCCCGTC CAAGTGTCAT ACTGCACTGA ATCTGTTGAG GATCTTCAAC 
GCCTCAGCTC CAGAGGTCGA TTCTACTTCT GTATTTCTCC CTCAGCAGCG AGCCCTCTTT 
GTACTTCGCC ACGTCGCTGG ATGGCTCACA AGTGATGATG TCGACGAGGA CAAGTTGCCT 
GAGGATATGG AGTTTAGAGT CTTGGAGCTC GAAGGGTCAG TGGCGCCAAT CGTGCAAGAC 
GTGGGTGGCG CACACTGGGA TGGGATCTTT GACTTGGTTG AAAACGGCTT AGAGAATTCG 
GGAATGGACG ACTCCTCTTC GCTCTGTTTG CTTTACCATA GCTTGTCCGT CCTTCAGCAG 
ATCAGGGATC TCTGTCAGAC CAACAAGTCT TTGAGAGCTT CTTGGACAAG TAAAACGAAT 
CATATGAAAC TTGTCTTGGA TCTATTCTTG CAATGTCGCT CAGCGAACTC TGTCCCTCTA 
CAATTAATTC AAAATCTTAT TCTTGACCTC CTGCCTGACG TCCCCGAGCA GGTCATGAAA 
GAGGCCAGCT TGACATCACT TTGTAATCTG ATATCCCTTT CGACATCTTC GGCTATCCAA 
ACTGCTGCTT ATCGAATCAT TTCTCAAGTT ATCAGACATC AAACGGTTGG ACTGGTCTTG 
GAAGTAGAAG CATCTGTAGC GCAAGCCCAA GAAGGCCATG CCGAAAGAAC CATTGAGATG 
GCAAAGGAGT TGGTGGATGT TGTTAAAATT GGAAGTGAAG TAAATTGGCA TGGCGAGCTT 
GGTGTATCCC TAGTCTTGGG ACAACTATTA GCTTGGATGT CTATATTGGA TTATTTCGAG 
GATGCTTCTC GGACTTTACG CTGGGCGTAC CTGGATCAAC TAAATACATC CAAGCTGATC 
ACGGACGGCC TCGTTCCAAT GCTCTTCTCC ATCCTTGGAG TATCTGAAAT GGGGGCCTGG 
AACTTCCCCG CCAGCCAATA CGCTGTAGAT GAGTTCTTCA CGGAGTTCCT TGACCCGGAG 
TCCTTGCCGG ATCTTTCCCC ACTTGCCTCT CATCTATTCT ACCGAGCACT GGTCACTATT 
CCATCTTCTG TCCGAGCATA TTACGAATCT CTTAAGGATC GCCAGCTATC TATGTCCATG 
CTCTCATTCA CTGCTCGGCA CTACTCTCCG GTAATCATTG CGCACGAGTT CTCTGCTTTA 
AGGGAACCGT CGGCGATGGC TCAGCTAACT GAAGAAGGCT TGAATGTGCG GATAGCGCAA 
GGTGGTGGTG CAACTATCGC AGCAGGAGCT GGGTCCGCTG AGGCAATTGC CAGCTACGTG 
GTCGATGAAC AGCCAATGGA AATTGGCATC AGATTACCGG CAGAGTTCCC GCTGAAAGGA 
GTCGATGTCA GAGATCTGCG AAGGGTGGGG GTGCCAGAGA ACAAGTGGCG AGGGTGGTTG 
ATGGGTTTAC AGCAAACTAT CACATCAAGG AATGGGTTGA TTCTCGAGGC ACTTACAATG 
TTTAAGAAAA ATGTTTCGCT GCATTTTGAG GGTGTCGTGG AATGTGCTAT CTGCTATTCT 
ATCATATCCC TTACGGACAG GACGCTTCCC ACCAAGCCAT GCCGAACGTG CAAGAATCGA 
TTCCATGCGA GCTGTCTCTT CAAATGGTTC AATTCGTCTC ATTCGAGCAG CTGTCCTCTT 
TGTCGAGCTC TGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNE03250 ( XM_024657244.1 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_024512987.1 Cryptococcus neoformans var. neoformans JEC21 conserved hypothetical protein (CNE03250), mRNA.

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CloneID	OCn08263
Clone ID Related Accession (Same CDS sequence)	XM_024657244.1
Accession Version	XM_024657244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523289600000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	conserved hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006687).

ATGCCCAAAG CAAACAAATC CTCGGCTTCA GCGGGGACTC GTAAGAAGCA TGCCGCCAAG 
AAGACTCACA GAGAGCACGA TGACGATTCT GATCATCAAC CAGAGTCGGG AACACCTCAA 
CCTTCGAGAA AACAGCGAGG GGAGAAAAAG CTCTCCAAGG CGCAAAAGAG AGCGATGCCG 
AAAGTGAAGC AATACATCCC TCCACCAAAA CCCCCTGCAC CTCCCATTCT GGATCCTCTC 
GACGGACAGG GCCTTGCGAG AACTTTGCCG GCGGATTTGG TCGTCGTACT GAGACGATTA 
GGAAAGAAGG ACGCTGTTAC AAGGCGCAAA GGTCTTGAGG AATTTAGGGA TGGCTGGGTG 
AAATACATTC TGAAGGAGAA ACCAACTGAA GAGGAAGAAA CTGAACGAGA AGTGAAGGAG 
ATTGCACTTC TAGCAGCTAT ACCTGTCTGG CTACATAACC TCTCAGGTCT TTTACAGTCC 
CCTTTCCACA GAGCTATTGC TCTCCTTATT CATACAGATC TTCTTTCTGT GCCTGCCCTT 
CGGGCTTCAA TTTTGGAAAG CCTAAATTTG GGCCTGTTAC CTGGTACCCA AAACCGTGAT 
GTTCTTGGGT CTTGGATGAT AGCTGCTTTA GAAGAAGGTC GAAGGGCAGG TGGACTAGGG 
CTCAGGGGAT GGGAATCGTC TATCCTCTGG AAGGCGAGCG GTGAAGAGGT TGGCGGTGAT 
ACAGACATTC GCATTGATCT CAAAGAACAC CTTTCCACTT TAATCGAGTA CCTTACTCTA 
TCTACCCTGA ACCCTTCGAT CCTACACGAG GACATCCATC CAATACCTGT AGCAGCTACC 
CCGGTGTCTT CTATTCCTTT GGGCAAAAAA GGTACTAACA AGCAAGGGAA ATCAAAAACA 
TCTTCTGCCC CACCGTCTCG ACAGACTGAA ACACCTGTAC CACCTCCGGA GGATCATGAA 
ATTCTTGAAG AAAGGATGAC AAGGTATCGA GTGGGCGGAC TTATAGGTCT TTCATGGCTC 
CTGCAACAAC TACCTCAAGT GGGATTGACA TCCTTGCCTG AAGATTTGGT CACTTTGCTA 
CGTCATCCTG CATTATGGCT TTCCTTGTCT TCAGAACAGG TTGATACAGT AAATTCACCT 
GGGTCCTTGG GCACTGCTCA GCCTCCTGTT AGAAAGGCAG GTTATGGGCT CCTCGATGTG 
CTTGTAAACT CTTACGAAAG TATTGTGGCT GAGCCCGAGA TGCTCAAAAT GGTGTCAAAC 
GCTGTCTTGA GTAGCTGTTG GAGAGAAAAA GAGGCAGTGG TATGGGAATC TGCAGGGTCC 
GCTATTGCCA AGTTTATCCG CAAATGGCCA GAATGCTGGA CACTTACCAC AGAGTCTGCT 
GCAGACCATC GTGATGGGCG CAATGATGGG CGCAATGATG GGGAAGAGGA GGTCGACAGT 
GAAGACGAGA ATGTTCAAAA CGCCGATGAC GAAGATGGCG CGAATCCGGC CACTCTCCTT 
CAGCTTTCTT CACCATACTA CTCTGATTTC TTGGACTTCA CATCCACTAT TTGCCCTTAT 
ATTCCTCATA TCACTTACCC CATAATTCTT GTTGTTCTCT CCACCCTTCC GCCCTCCTTA 
TTGCTGCTTG TTGGGCCATC CCTTCAGCTC CAGACGTTAT TCTCTCATCT TTGGGCCCCC 
CTGGATTCTC GCTTACTTTC CACGCATTGT CTGCCAGGTC AAAAGTCTGC ATTCCAAGTG 
TTTTTCCAGG CTATATTGGA GTGTACCATA TTCCTCATTG GCAAGTCATG GAAAGAGAAG 
GACGGAAAAG AGGCTGCAAT GTGGTTGGTG AAGGAGCAGC TAGGCAACAG AGCATGGAAA 
GAGGGCGTTC TGCTTATGGG CGGAAGGGCT GGTGGAAGGC ATGTCCCTCG AGGATGTACC 
GAAGAGAGTG AAGCTTCTGC ATTTGGCAGC ACTCTTGGCC GTCTGGCATC TATTGATCAA 
CTACTCTTAG ACCAAATCCG GGTCGTGCTT ACCCAGACGC TATCTGATGC TTGTTTCCCG 
CAAGAGAACC CGGAGAGCGG TCCTATGGAG GTAAGTATTT TACCGAGAAG CTTGAGTATA 
CTCTCTGCTA TAAGAGACGC GATTGCTGCT AGTCAAGTGA TCGGCCTAGT GGATCAATTA 
ATGGAGGATT TGATCGAAAA ATTATCTGAA GTGTTTGTGG GCTCTTCAAA GAGCGACAAT 
CCCTCCCATG CAATCATGGC GGAGACATTA GTAGATGCTC TGAAGAACCA TCACCATTTG 
GTGCACAGCG ACACAATAGA GAAACTTTTG GGCCAAGTCA AAGATTCGTC CCACGAGCTA 
CTCATAATCC TGCACCCACC TTTGTTCATT GCTTATCTAG ACAGCCTCTG CTCCAAGTTG 
CCGTCTGCCG AGCGACATGT TTGCCAACAA GCTCTTTCTA CCGTGCTGCA GTCTTCCGAA 
ATAGACTCTT CGCAGCGCTT CACAGTTGCC AATGCTGTGT TGATTCTTGA CCATACCCAA 
CTTCTACAAT CGGGCTCACT GGATCAAATT GTTAAAGAAG CCACGCATGA CGCTCTAGCA 
GGCGGATCAC CTATTGCCAT CCCCGTGGTC ACCGCATCTC TCAAATCCCT TTGCTACCTA 
TCAAAATCTA CCCTGGATGA GGTGTTATCT GTTACCTGCA GCGTCATTCG TGAATCAACC 
AAAAAGCTTC TGACCACCGA TTTCGTTGAG CTTGGTGTTC CGCAGGCGGC CTTCGAGATC 
TTTGCTGCCT ATGCCACAAA CCATCTTCAG GAAGTCATCG AATCGGAGCA TTTGATTTCG 
GGTCTGGTCG GCGTTCATCA TGTCCTATTC GTTTTACCTA GATTACCTGG TCACATTGCC 
TCAGCCGAGT CAAAGGCTAG CGGCCTGCTC AGTTTATGGT CCAAGCTGGG TGAACTGGTT 
GTGGAATACC AGGAGAAGGT CCTCCAAAGA GTGTACAGTG CCTTAAAAGG AGAAATGGGT 
GATGTAGAAG TCAGGGTGGA CCCTGAGGTG CTCATCGACG TGGCTCTTGC AACAACCCTC 
GGGTCCCACT CTGCTCCTTC CCTCCAAGCG CTTGTCCTGG CGTTGCTTCC CGAAGTGATC 
AGTATCATTC AGGATCTCGC TCCTCATACA TCCCAACCTC CCCATCCGTC TCTGCCTATC 
GTGGACCCAC TTATACCGTT TGTCCCCCAT GGTGACGATG ACCTTGACTC TATTCAACCA 
GAGTTTGATA GCCTTGGTCG AGCTCAGACA GCCAGATACG CCGAGGCGGC GGTAGCTCTA 
CTCCGAGCAG ACAGAAGCCT TGTTAAGGAT GAACCTGCTT TATTGCAAAT GATTTTAGCT 
GTCAGTCGAC TAGCACAGGA TGCTCTGACT GTTCCCGGAG CATCCAGAGG GCTGTTTTCG 
AAAGAGACAC CTTCCACCCG CCTAGCTGAT CTGGTTCGAG AAGTAGAAGG GGCCGTTTCT 
TTCGCTCTTG GATATGTGGA CGAGGTTGAG CCCTCTTGGC ACCATGCCAC CATTCATGCT 
CTCAAATCTG GATCTGTGCC ACATGATTCC GACTTGTTAC AAAGCTTAAT GGTTGCGCTG 
CAGAAAGAAG TGACTGCAAA TGGTAACGAC GTGGATGCCA GGTCATTTAG GGATGTGCTT 
TACAAGCACA TGAGACAAAT AGGAGCGGGT GTCCAAGAAG GGGAGGCATG GCTAAACTAT 
GCCATGAGTC TGGTTGACAG ATCACCACAG CTCAGTCTCG CCATAATAAC TGCCATCAAA 
CCTCTCATGC TTGACACACA GGCTTTTGCC ACTGCCCAAA CCCGTCTCGC ATCGGCTCTT 
ACAACCATTC CCCCGTCCAA GTGTCATACT GCACTGAATC TGTTGAGGAT CTTCAACGCC 
TCAGCTCCAG AGGTCGATTC TACTTCTGTA TTTCTCCCTC AGCAGCGAGC CCTCTTTGTA 
CTTCGCCACG TCGCTGGATG GCTCACAAGT GATGATGTCG ACGAGGACAA GTTGCCTGAG 
GATATGGAGT TTAGAGTCTT GGAGCTCGAA GGGTCAGTGG CGCCAATCGT GCAAGACGTG 
GGTGGCGCAC ACTGGGATGG GATCTTTGAC TTGGTTGAAA ACGGCTTAGA GAATTCGGGA 
ATGGACGACT CCTCTTCGCT CTGTTTGCTT TACCATAGCT TGTCCGTCCT TCAGCAGATC 
AGGGATCTCT GTCAGACCAA CAAGTCTTTG AGAGCTTCTT GGACAAGTAA AACGAATCAT 
ATGAAACTTG TCTTGGATCT ATTCTTGCAA TGTCGCTCAG CGAACTCTGT CCCTCTACAA 
TTAATTCAAA ATCTTATTCT TGACCTCCTG CCTGACGTCC CCGAGCAGGT CATGAAAGAG 
GCCAGCTTGA CATCACTTTG TAATCTGATA TCCCTTTCGA CATCTTCGGC TATCCAAACT 
GCTGCTTATC GAATCATTTC TCAAGTTATC AGACATCAAA CGGTTGGACT GGTCTTGGAA 
GTAGAAGCAT CTGTAGCGCA AGCCCAAGAA GGCCATGCCG AAAGAACCAT TGAGATGGCA 
AAGGAGTTGG TGGATGTTGT TAAAATTGGA AGTGAAGTAA ATTGGCATGG CGAGCTTGGT 
GTATCCCTAG TCTTGGGACA ACTATTAGCT TGGATGTCTA TATTGGATTA TTTCGAGGAT 
GCTTCTCGGA CTTTACGCTG GGCGTACCTG GATCAACTAA ATACATCCAA GCTGATCACG 
GACGGCCTCG TTCCAATGCT CTTCTCCATC CTTGGAGTAT CTGAAATGGG GGCCTGGAAC 
TTCCCCGCCA GCCAATACGC TGTAGATGAG TTCTTCACGG AGTTCCTTGA CCCGGAGTCC 
TTGCCGGATC TTTCCCCACT TGCCTCTCAT CTATTCTACC GAGCACTGGT CACTATTCCA 
TCTTCTGTCC GAGCATATTA CGAATCTCTT AAGGATCGCC AGCTATCTAT GTCCATGCTC 
TCATTCACTG CTCGGCACTA CTCTCCGGTA ATCATTGCGC ACGAGTTCTC TGCTTTAAGG 
GAACCGTCGG CGATGGCTCA GCTAACTGAA GAAGGCTTGA ATGTGCGGAT AGCGCAAGGT 
GGTGGTGCAA CTATCGCAGC AGGAGCTGGG TCCGCTGAGG CAATTGCCAG CTACGTGGTC 
GATGAACAGC CAATGGAAAT TGGCATCAGA TTACCGGCAG AGTTCCCGCT GAAAGGAGTC 
GATGTCAGAG ATCTGCGAAG GGTGGGGGTG CCAGAGAACA AGTGGCGAGG GTGGTTGATG 
GGTTTACAGC AAACTATCAC ATCAAGGAAT GGGTTGATTC TCGAGGCACT TACAATGTTT 
AAGAAAAATG TTTCGCTGCA TTTTGAGGGT GTCGTGGAAT GTGCTATCTG CTATTCTATC 
ATATCCCTTA CGGACAGGAC GCTTCCCACC AAGCCATGCC GAACGTGCAA GAATCGATTC 
CATGCGAGCT GTCTCTTCAA ATGGTTCAAT TCGTCTCATT CGAGCAGCTG TCCTCTTTGT 
CGAGCTCTGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024512987.1
CDS	211..5745
Translation	MPKANKSSASAGTRKKHAAKKTHREHDDDSDHQPESGTPQPSRKQRGEKKLSKAQKRAMPKVKQYIPPPKPPAPPILDPLDGQGLARTLPADLVVVLRRLGKKDAVTRRKGLEEFRDGWVKYILKEKPTEEEETEREVKEIALLAAIPVWLHNLSGLLQSPFHRAIALLIHTDLLSVPALRASILESLNLGLLPGTQNRDVLGSWMIAALEEGRRAGGLGLRGWESSILWKASGEEVGGDTDIRIDLKEHLSTLIEYLTLSTLNPSILHEDIHPIPVAATPVSSIPLGKKGTNKQGKSKTSSAPPSRQTETPVPPPEDHEILEERMTRYRVGGLIGLSWLLQQLPQVGLTSLPEDLVTLLRHPALWLSLSSEQVDTVNSPGSLGTAQPPVRKAGYGLLDVLVNSYESIVAEPEMLKMVSNAVLSSCWREKEAVVWESAGSAIAKFIRKWPECWTLTTESAADHRDGRNDGRNDGEEEVDSEDENVQNADDEDGANPATLLQLSSPYYSDFLDFTSTICPYIPHITYPIILVVLSTLPPSLLLLVGPSLQLQTLFSHLWAPLDSRLLSTHCLPGQKSAFQVFFQAILECTIFLIGKSWKEKDGKEAAMWLVKEQLGNRAWKEGVLLMGGRAGGRHVPRGCTEESEASAFGSTLGRLASIDQLLLDQIRVVLTQTLSDACFPQENPESGPMEVSILPRSLSILSAIRDAIAASQVIGLVDQLMEDLIEKLSEVFVGSSKSDNPSHAIMAETLVDALKNHHHLVHSDTIEKLLGQVKDSSHELLIILHPPLFIAYLDSLCSKLPSAERHVCQQALSTVLQSSEIDSSQRFTVANAVLILDHTQLLQSGSLDQIVKEATHDALAGGSPIAIPVVTASLKSLCYLSKSTLDEVLSVTCSVIRESTKKLLTTDFVELGVPQAAFEIFAAYATNHLQEVIESEHLISGLVGVHHVLFVLPRLPGHIASAESKASGLLSLWSKLGELVVEYQEKVLQRVYSALKGEMGDVEVRVDPEVLIDVALATTLGSHSAPSLQALVLALLPEVISIIQDLAPHTSQPPHPSLPIVDPLIPFVPHGDDDLDSIQPEFDSLGRAQTARYAEAAVALLRADRSLVKDEPALLQMILAVSRLAQDALTVPGASRGLFSKETPSTRLADLVREVEGAVSFALGYVDEVEPSWHHATIHALKSGSVPHDSDLLQSLMVALQKEVTANGNDVDARSFRDVLYKHMRQIGAGVQEGEAWLNYAMSLVDRSPQLSLAIITAIKPLMLDTQAFATAQTRLASALTTIPPSKCHTALNLLRIFNASAPEVDSTSVFLPQQRALFVLRHVAGWLTSDDVDEDKLPEDMEFRVLELEGSVAPIVQDVGGAHWDGIFDLVENGLENSGMDDSSSLCLLYHSLSVLQQIRDLCQTNKSLRASWTSKTNHMKLVLDLFLQCRSANSVPLQLIQNLILDLLPDVPEQVMKEASLTSLCNLISLSTSSAIQTAAYRIISQVIRHQTVGLVLEVEASVAQAQEGHAERTIEMAKELVDVVKIGSEVNWHGELGVSLVLGQLLAWMSILDYFEDASRTLRWAYLDQLNTSKLITDGLVPMLFSILGVSEMGAWNFPASQYAVDEFFTEFLDPESLPDLSPLASHLFYRALVTIPSSVRAYYESLKDRQLSMSMLSFTARHYSPVIIAHEFSALREPSAMAQLTEEGLNVRIAQGGGATIAAGAGSAEAIASYVVDEQPMEIGIRLPAEFPLKGVDVRDLRRVGVPENKWRGWLMGLQQTITSRNGLILEALTMFKKNVSLHFEGVVECAICYSIISLTDRTLPTKPCRTCKNRFHASCLFKWFNSSHSSSCPLCRALF

Target ORF information:

RefSeq Version	XM_024657244.1
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 conserved hypothetical protein (CNE03250), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024657244.1

ORF Insert Sequence:

ATGCCCAAAG CAAACAAATC CTCGGCTTCA GCGGGGACTC GTAAGAAGCA TGCCGCCAAG 
AAGACTCACA GAGAGCACGA TGACGATTCT GATCATCAAC CAGAGTCGGG AACACCTCAA 
CCTTCGAGAA AACAGCGAGG GGAGAAAAAG CTCTCCAAGG CGCAAAAGAG AGCGATGCCG 
AAAGTGAAGC AATACATCCC TCCACCAAAA CCCCCTGCAC CTCCCATTCT GGATCCTCTC 
GACGGACAGG GCCTTGCGAG AACTTTGCCG GCGGATTTGG TCGTCGTACT GAGACGATTA 
GGAAAGAAGG ACGCTGTTAC AAGGCGCAAA GGTCTTGAGG AATTTAGGGA TGGCTGGGTG 
AAATACATTC TGAAGGAGAA ACCAACTGAA GAGGAAGAAA CTGAACGAGA AGTGAAGGAG 
ATTGCACTTC TAGCAGCTAT ACCTGTCTGG CTACATAACC TCTCAGGTCT TTTACAGTCC 
CCTTTCCACA GAGCTATTGC TCTCCTTATT CATACAGATC TTCTTTCTGT GCCTGCCCTT 
CGGGCTTCAA TTTTGGAAAG CCTAAATTTG GGCCTGTTAC CTGGTACCCA AAACCGTGAT 
GTTCTTGGGT CTTGGATGAT AGCTGCTTTA GAAGAAGGTC GAAGGGCAGG TGGACTAGGG 
CTCAGGGGAT GGGAATCGTC TATCCTCTGG AAGGCGAGCG GTGAAGAGGT TGGCGGTGAT 
ACAGACATTC GCATTGATCT CAAAGAACAC CTTTCCACTT TAATCGAGTA CCTTACTCTA 
TCTACCCTGA ACCCTTCGAT CCTACACGAG GACATCCATC CAATACCTGT AGCAGCTACC 
CCGGTGTCTT CTATTCCTTT GGGCAAAAAA GGTACTAACA AGCAAGGGAA ATCAAAAACA 
TCTTCTGCCC CACCGTCTCG ACAGACTGAA ACACCTGTAC CACCTCCGGA GGATCATGAA 
ATTCTTGAAG AAAGGATGAC AAGGTATCGA GTGGGCGGAC TTATAGGTCT TTCATGGCTC 
CTGCAACAAC TACCTCAAGT GGGATTGACA TCCTTGCCTG AAGATTTGGT CACTTTGCTA 
CGTCATCCTG CATTATGGCT TTCCTTGTCT TCAGAACAGG TTGATACAGT AAATTCACCT 
GGGTCCTTGG GCACTGCTCA GCCTCCTGTT AGAAAGGCAG GTTATGGGCT CCTCGATGTG 
CTTGTAAACT CTTACGAAAG TATTGTGGCT GAGCCCGAGA TGCTCAAAAT GGTGTCAAAC 
GCTGTCTTGA GTAGCTGTTG GAGAGAAAAA GAGGCAGTGG TATGGGAATC TGCAGGGTCC 
GCTATTGCCA AGTTTATCCG CAAATGGCCA GAATGCTGGA CACTTACCAC AGAGTCTGCT 
GCAGACCATC GTGATGGGCG CAATGATGGG CGCAATGATG GGGAAGAGGA GGTCGACAGT 
GAAGACGAGA ATGTTCAAAA CGCCGATGAC GAAGATGGCG CGAATCCGGC CACTCTCCTT 
CAGCTTTCTT CACCATACTA CTCTGATTTC TTGGACTTCA CATCCACTAT TTGCCCTTAT 
ATTCCTCATA TCACTTACCC CATAATTCTT GTTGTTCTCT CCACCCTTCC GCCCTCCTTA 
TTGCTGCTTG TTGGGCCATC CCTTCAGCTC CAGACGTTAT TCTCTCATCT TTGGGCCCCC 
CTGGATTCTC GCTTACTTTC CACGCATTGT CTGCCAGGTC AAAAGTCTGC ATTCCAAGTG 
TTTTTCCAGG CTATATTGGA GTGTACCATA TTCCTCATTG GCAAGTCATG GAAAGAGAAG 
GACGGAAAAG AGGCTGCAAT GTGGTTGGTG AAGGAGCAGC TAGGCAACAG AGCATGGAAA 
GAGGGCGTTC TGCTTATGGG CGGAAGGGCT GGTGGAAGGC ATGTCCCTCG AGGATGTACC 
GAAGAGAGTG AAGCTTCTGC ATTTGGCAGC ACTCTTGGCC GTCTGGCATC TATTGATCAA 
CTACTCTTAG ACCAAATCCG GGTCGTGCTT ACCCAGACGC TATCTGATGC TTGTTTCCCG 
CAAGAGAACC CGGAGAGCGG TCCTATGGAG GTAAGTATTT TACCGAGAAG CTTGAGTATA 
CTCTCTGCTA TAAGAGACGC GATTGCTGCT AGTCAAGTGA TCGGCCTAGT GGATCAATTA 
ATGGAGGATT TGATCGAAAA ATTATCTGAA GTGTTTGTGG GCTCTTCAAA GAGCGACAAT 
CCCTCCCATG CAATCATGGC GGAGACATTA GTAGATGCTC TGAAGAACCA TCACCATTTG 
GTGCACAGCG ACACAATAGA GAAACTTTTG GGCCAAGTCA AAGATTCGTC CCACGAGCTA 
CTCATAATCC TGCACCCACC TTTGTTCATT GCTTATCTAG ACAGCCTCTG CTCCAAGTTG 
CCGTCTGCCG AGCGACATGT TTGCCAACAA GCTCTTTCTA CCGTGCTGCA GTCTTCCGAA 
ATAGACTCTT CGCAGCGCTT CACAGTTGCC AATGCTGTGT TGATTCTTGA CCATACCCAA 
CTTCTACAAT CGGGCTCACT GGATCAAATT GTTAAAGAAG CCACGCATGA CGCTCTAGCA 
GGCGGATCAC CTATTGCCAT CCCCGTGGTC ACCGCATCTC TCAAATCCCT TTGCTACCTA 
TCAAAATCTA CCCTGGATGA GGTGTTATCT GTTACCTGCA GCGTCATTCG TGAATCAACC 
AAAAAGCTTC TGACCACCGA TTTCGTTGAG CTTGGTGTTC CGCAGGCGGC CTTCGAGATC 
TTTGCTGCCT ATGCCACAAA CCATCTTCAG GAAGTCATCG AATCGGAGCA TTTGATTTCG 
GGTCTGGTCG GCGTTCATCA TGTCCTATTC GTTTTACCTA GATTACCTGG TCACATTGCC 
TCAGCCGAGT CAAAGGCTAG CGGCCTGCTC AGTTTATGGT CCAAGCTGGG TGAACTGGTT 
GTGGAATACC AGGAGAAGGT CCTCCAAAGA GTGTACAGTG CCTTAAAAGG AGAAATGGGT 
GATGTAGAAG TCAGGGTGGA CCCTGAGGTG CTCATCGACG TGGCTCTTGC AACAACCCTC 
GGGTCCCACT CTGCTCCTTC CCTCCAAGCG CTTGTCCTGG CGTTGCTTCC CGAAGTGATC 
AGTATCATTC AGGATCTCGC TCCTCATACA TCCCAACCTC CCCATCCGTC TCTGCCTATC 
GTGGACCCAC TTATACCGTT TGTCCCCCAT GGTGACGATG ACCTTGACTC TATTCAACCA 
GAGTTTGATA GCCTTGGTCG AGCTCAGACA GCCAGATACG CCGAGGCGGC GGTAGCTCTA 
CTCCGAGCAG ACAGAAGCCT TGTTAAGGAT GAACCTGCTT TATTGCAAAT GATTTTAGCT 
GTCAGTCGAC TAGCACAGGA TGCTCTGACT GTTCCCGGAG CATCCAGAGG GCTGTTTTCG 
AAAGAGACAC CTTCCACCCG CCTAGCTGAT CTGGTTCGAG AAGTAGAAGG GGCCGTTTCT 
TTCGCTCTTG GATATGTGGA CGAGGTTGAG CCCTCTTGGC ACCATGCCAC CATTCATGCT 
CTCAAATCTG GATCTGTGCC ACATGATTCC GACTTGTTAC AAAGCTTAAT GGTTGCGCTG 
CAGAAAGAAG TGACTGCAAA TGGTAACGAC GTGGATGCCA GGTCATTTAG GGATGTGCTT 
TACAAGCACA TGAGACAAAT AGGAGCGGGT GTCCAAGAAG GGGAGGCATG GCTAAACTAT 
GCCATGAGTC TGGTTGACAG ATCACCACAG CTCAGTCTCG CCATAATAAC TGCCATCAAA 
CCTCTCATGC TTGACACACA GGCTTTTGCC ACTGCCCAAA CCCGTCTCGC ATCGGCTCTT 
ACAACCATTC CCCCGTCCAA GTGTCATACT GCACTGAATC TGTTGAGGAT CTTCAACGCC 
TCAGCTCCAG AGGTCGATTC TACTTCTGTA TTTCTCCCTC AGCAGCGAGC CCTCTTTGTA 
CTTCGCCACG TCGCTGGATG GCTCACAAGT GATGATGTCG ACGAGGACAA GTTGCCTGAG 
GATATGGAGT TTAGAGTCTT GGAGCTCGAA GGGTCAGTGG CGCCAATCGT GCAAGACGTG 
GGTGGCGCAC ACTGGGATGG GATCTTTGAC TTGGTTGAAA ACGGCTTAGA GAATTCGGGA 
ATGGACGACT CCTCTTCGCT CTGTTTGCTT TACCATAGCT TGTCCGTCCT TCAGCAGATC 
AGGGATCTCT GTCAGACCAA CAAGTCTTTG AGAGCTTCTT GGACAAGTAA AACGAATCAT 
ATGAAACTTG TCTTGGATCT ATTCTTGCAA TGTCGCTCAG CGAACTCTGT CCCTCTACAA 
TTAATTCAAA ATCTTATTCT TGACCTCCTG CCTGACGTCC CCGAGCAGGT CATGAAAGAG 
GCCAGCTTGA CATCACTTTG TAATCTGATA TCCCTTTCGA CATCTTCGGC TATCCAAACT 
GCTGCTTATC GAATCATTTC TCAAGTTATC AGACATCAAA CGGTTGGACT GGTCTTGGAA 
GTAGAAGCAT CTGTAGCGCA AGCCCAAGAA GGCCATGCCG AAAGAACCAT TGAGATGGCA 
AAGGAGTTGG TGGATGTTGT TAAAATTGGA AGTGAAGTAA ATTGGCATGG CGAGCTTGGT 
GTATCCCTAG TCTTGGGACA ACTATTAGCT TGGATGTCTA TATTGGATTA TTTCGAGGAT 
GCTTCTCGGA CTTTACGCTG GGCGTACCTG GATCAACTAA ATACATCCAA GCTGATCACG 
GACGGCCTCG TTCCAATGCT CTTCTCCATC CTTGGAGTAT CTGAAATGGG GGCCTGGAAC 
TTCCCCGCCA GCCAATACGC TGTAGATGAG TTCTTCACGG AGTTCCTTGA CCCGGAGTCC 
TTGCCGGATC TTTCCCCACT TGCCTCTCAT CTATTCTACC GAGCACTGGT CACTATTCCA 
TCTTCTGTCC GAGCATATTA CGAATCTCTT AAGGATCGCC AGCTATCTAT GTCCATGCTC 
TCATTCACTG CTCGGCACTA CTCTCCGGTA ATCATTGCGC ACGAGTTCTC TGCTTTAAGG 
GAACCGTCGG CGATGGCTCA GCTAACTGAA GAAGGCTTGA ATGTGCGGAT AGCGCAAGGT 
GGTGGTGCAA CTATCGCAGC AGGAGCTGGG TCCGCTGAGG CAATTGCCAG CTACGTGGTC 
GATGAACAGC CAATGGAAAT TGGCATCAGA TTACCGGCAG AGTTCCCGCT GAAAGGAGTC 
GATGTCAGAG ATCTGCGAAG GGTGGGGGTG CCAGAGAACA AGTGGCGAGG GTGGTTGATG 
GGTTTACAGC AAACTATCAC ATCAAGGAAT GGGTTGATTC TCGAGGCACT TACAATGTTT 
AAGAAAAATG TTTCGCTGCA TTTTGAGGGT GTCGTGGAAT GTGCTATCTG CTATTCTATC 
ATATCCCTTA CGGACAGGAC GCTTCCCACC AAGCCATGCC GAACGTGCAA GAATCGATTC 
CATGCGAGCT GTCTCTTCAA ATGGTTCAAT TCGTCTCATT CGAGCAGCTG TCCTCTTTGT 
CGAGCTCTGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)
Loftus BJ,Fung E,Roncaglia P,Rowley D,Amedeo P,Bruno D,Vamathevan J,Miranda M,Anderson IJ,Fraser JA,Allen JE,Bosdet IE,Brent MR,Chiu R,Doering TL,Donlin MJ,D'Souza CA,Fox DS,Grinberg V,Fu J,Fukushima M,Haas BJ,Huang JC,Janbon G,Jones SJ,Koo HL,Krzywinski MI,Kwon-Chung JK,Lengeler KB,Maiti R,Marra MA,Marra RE,Mathewson CA,Mitchell TG,Pertea M,Riggs FR,Salzberg SL,Schein JE,Shvartsbeyn A,Shin H,Shumway M,Specht CA,Suh BB,Tenney A,Utterback TR,Wickes BL,Wortman JR,Wye NH,Kronstad JW,Lodge JK,Heitman J,Davis RW,Fraser CM,Hyman RW

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CNE03250 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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