CNC05860 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNC05860
Entrez Gene ID	3256724
Full Name	metal resistance protein ycf1
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

mRNA and Protein(s)

mRNA	Protein	Name
XM_569810.2	XP_569810.1	metal resistance protein ycf1, putative
XM_569810.1	XP_569810.1	metal resistance protein ycf1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Loftus BJ, Fung E, Roncaglia P, Rowley D, Amedeo P, Bruno D, Vamathevan J, Miranda M, Anderson IJ, Fraser JA, Allen JE, Bosdet IE, Brent MR, Chiu R, Doering TL, Donlin MJ, D'Souza CA, Fox DS, Grinberg V, Fu J, Fukushima M, Haas BJ, Huang JC, Janbon G, Jones SJ, Koo HL, Krzywinski MI, Kwon-Chung JK, Lengeler KB, Maiti R, Marra MA, Marra RE, Mathewson CA, Mitchell TG, Pertea M, Riggs FR, Salzberg SL, Schein JE, Shvartsbeyn A, Shin H, Shumway M, Specht CA, Suh BB, Tenney A, Utterback TR, Wickes BL, Wortman JR, Wye NH, Kronstad JW, Lodge JK, Heitman J, Davis RW, Fraser CM, Hyman RW
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNC05860 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNC05860 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCn03456	XM_569810.2 Latest version!	Cryptococcus neoformans var. neoformans JEC21 metal resistance protein ycf1, putative (CNC05860), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCn03456	XM_569810.1	Cryptococcus neoformans var. neoformans JEC21 metal resistance protein ycf1, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNC05860 ( XM_569810.2 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_569810.1 Cryptococcus neoformans var. neoformans JEC21 metal resistance protein ycf1, putative (CNC05860), mRNA.

Price & Availability↑

CloneID	OCn03456
Clone ID Related Accession (Same CDS sequence)	XM_569810.2 , XM_569810.1
Accession Version	XM_569810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523289600000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	metal resistance protein ycf1, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006685). On Apr 11, 2018 this sequence version replaced XM_569810.1.

ATGACTGCCC AAGCAGCCTT CGAGGCCTTC GCCTCGAAGC ACACCCGCCC GTATGACTGT 
ACCTTTCCTC CAAAAATTGT CTCGTCCCAT AGGGATCTCG ACTTTACCCA GTGCTTCGAG 
CACGCCGTTT TACTGCCCGC CCCTCTCGCC TTCTTCACCT TGCTCGCACT TTCCCAGATC 
TTGAGAATCA CAAGACAACT CAAGAAAGGC GGACTGAACG GTGGTCTTGC ATGGATTACG 
AGGTCCAGGA GAAGTGAGAG AATATGCTCC ACCAAGGTGG GCTTCCTCGC AGCTTCTGCC 
GTCTTCTCCC TAGTTTCATT ATGCCTTACA TTCAAAAACA TCTCTGTAAA TCTTCTTTCC 
GCCGCCCACT ACTTGCTGCT CTTCGTGACT CTCTTATCGC TTATCCACCT CACCGCCTTG 
AACCACCACA CTTCCCGAAC TTCATCGACT ATTATTCTTC TCTTCTATCC CACCTATCTT 
CTCGTATTTG CCATCCGCCT TAGGACAATG ATCGTTTCTG GTGCCCTCAA CTCTGGTCTT 
ACCCATTCTA CTTCAGGGCG CTTGTTGTTG GCAAGACAGG CTTTCTGGTT CGCAAGTATC 
ATTGCGGCTC TTCCCGCTTT TCTGCTCGAA CTATACTCTC CTGAAAAGAA ATGGCAGAGA 
TGGAATGCTC CTTGGAAGAG AAACGGCAAA ATCGCCTTGG AGGATGACGA GGAAGAAGAA 
GCGGATGGAA TCGAGAACTT GGACGGCCAA GGTGCTGTGC CGGGCAAGAA CGCTTACGGC 
GATGTCGAGA GCCCCGTTTC CACCGCCAAT ATCTATGAAA TTCTGACTTT TTCTTGGCTC 
ACTCCTCTTC TCTCTCTTGG TACCCGCAAG TACCTTGGTG AAGAAGACAT GTGGGCGCTC 
CCCTCTGAAG ACTCTGCCGA AGCTCTTTCC AACCGTCTTG CGGAAACCTG GAAATCGCAA 
GCTGAACAGG TCAAAGCTGG CAAAAAGAAG AGCGCTTCTC TCAAGATCGC CCTTGTCAAG 
GCTTACGGTG GTCCTTATAT TGTTGCTGGT ATCCTCAAGG CCTTGTATGA TATGATCAAC 
TTTCTGCAGC CTCAACTTTT GAGGTTGTTA CTCAACTTTG TCTCGAGCTA CACTAGTGAA 
CGTCCAATGC CGCCTGTTAC TGGGTACGCT ATTGCCGTTT TGATGTTTAT CAGCGCAAAC 
GTAGGAACCG CCGTTTTGCA CCAGTACTTC CAGCGATGCT TTACCACCAC TATGCGTATC 
CGGGGTGGAC TTGTCACTTT GATTTACCGC AAGGCACTTG TCCTTAGCAA CGGCGAAAAG 
TCTGGCAGGA CTACTGGTGA CATTGTCAAC CTCCAGAGCG TTGATGCCGT CAGGATTGCA 
GATGTCTGTC AGTACGGTCA TATTGCTTGG TCAGGCCCTT TCCAGATTCT CATTGCATTC 
ATTTCTCTCT ACAGGCTTGT CGGCTGGCAA GCGTTTATGG GCGTTGCTGT CATGGTGGTC 
AGTTTGCCAG CAAACACTCT CATTTCTCGA TTCAATAAGC GCTACCACCG GCGATTAATG 
AAGGTCAAGG ACACTCGTAC TCGTACCATG AACGAGATCC TCAACAACAT CAAGTCTATC 
AAGCTGTACG GCTGGGAGCA GGCATTTGCC AACAAGATCT ACGACATCCG TAACAACCAA 
GAACTCAAGA TGTTGCGCAA GATTGGTATC GTCATGGCTG GAAGTAACTT TATCTGGCAA 
GGTACCCCCT TCCTTGTTGC ATTCTCTACC TTTGCCACGT TTGCTTTCAC CAGCGACAAA 
CCTTTGACCA GTGAGATCAT TTTCCCCGCC ATCTCTCTCT TCCAGCTTCT CTCCTTCCCC 
ATGGCCATGT TTGCCAACAT CCTCAACTCT ATCATCGAAG CTTCCGTTTC AGTCGGCCGT 
TTGGAAAGTT TCCTTGCTGC TGATGAACTC AACCCTAATG CCCGCACTGT CATTCGACCT 
TCTGAAGATC CTCAGGGTGA GCCTCAAAAG GGCGATACCG TTGTTAGCAT TAAGAATGGC 
GAATTCCGAT GGCTCGAGGG CTCTACGGAG CCCATCCTGC AGGACATCGA CCTTGAAGTT 
AAAAAGGGTG AGCTTATCGC TCTTATCGGC CGAGTTGGTG ACGGCAAATC ATCTCTGTTG 
GGCGCGATTC TTGGTGAAAT GACTCGCTCT GACGGCTCTG TCACTCTCCG CGGCGAGGTC 
GCATACTTTT CGCAAAACTC TTGGATCCTC TCTGCGACTG TCAAGGATAA CATTGTGTTT 
GGCCACCGAT TCGACAAACA GTTTTACGAA CAGGTGTTGG ATGCTTGTGC TTTGAGGCAA 
GACCTTGCGG TACTTCCTAG CGGCGATATG ACCGAGGTCG GTGAAAAGGG TGTTTCCCTC 
TCTGGTGGTC AAAAGGCTCG TATCTGTCTT GCTAGAGCTG TCTATGCTCG TGCCGATATT 
TACCTTCTTG ACGATCCTCT TGCGGCCGTT GATTCTCATG TCGGTCGACA CATTTTCGAC 
AAGGTCATCG GGCCTAACGG TCTCCTTGCT TCAAAAGCCC GAATTCTCTG TACCAACGCT 
GTCACTTTTC TTCCCCAGGC CGACCAAATC ATCTCTCTCC GTAGAGGTAT TGTGCTTGAG 
CGGGGTACTT ATGAAGAGGC CATGAACGAT TCGTCCTCTG AGCTTTACAA GCTTATCACT 
GGTTTGGGCA AGCAATCTGC CATGGGCGAT GGACAAGATT CTGGACAAGG TTCTGGTGCT 
ACTACACCTA CGGTTGTTGG ACAAGAGGAG GTGGTGGTAG TTGAGGAACC AGAGGGTGTG 
GAAGACAGCG AAGAAGCTGA GATTGTCACT GGCGCTGATT CTCCTAAACA ACGCAAAGCG 
TACCGACAGC TCAGCCGCGA CATCATGCGT CGATCTTCTG TCGTTTCTCT CCGTACTGCC 
AAACGTGACG CCCTGCGTGA CCTACGCGAA AGCGCTAAAC CAAAGGAACG CTCTGAAAAG 
GGCAACGTGA AGCGAGAAGT CTACCGGGAA TTCATCAAGG CCTCTTCCAA ATGGGGCGTT 
GCCGTCTTTA TCGGCGCGAT GAGTCTCGCG CAGGGTTTGA ACATTCTTTC CAACTTTGTA 
CTTCGTGCTT GGGCCAGTGC CAACTCTGAT AGTTCAGGCG AGATGCCGAG TGTGACCAAA 
TACCTCCTCA TCTACGGTAT CGTCGGTATT TCTGGTTCCG TGGCCAACGT TGTCAGCGTC 
GCCACGCTCA AGATTGTTTG TGCCCTCAAG TCTAGTAGGA GTCTCCATGA CAGGTCATTT 
GGGGCTTTGA TGAAGAGTCC CTTGTCATTC TTCGAGCTTA CACCAACAGG ACGAATCCTC 
AACTTGTTCT CTAGGGATAT CTTTGTTATT GATGAAGTCT TGATTATGGC TCTCGGTGGT 
TTCTTCCGTA CTTCCGTATC AGTCCTCGGT ACTGTGGTAG TCATTGCTAT GGGCACGCCT 
TTGGTGTTGC TCGTGTTCAT CCCTCTTGGT TACCTCTACA GGATTGTCAT GAGATTCTAT 
CTTGCTACTT CTCGAGAGCT GAAACGGCTT GACGCCGTTT CTCGATCTCC CGTCTTCTCC 
TTCTTTGGCG AAACCCTTTC CGGTCTGCCT GTTATCCGAG GATATGGCCA ATCTGCCCGA 
TTCATTGCCA ACAACGAAGC CCGTGTGGAT CGAAACCAGG CGTGTTTCAT GCCGGCTATG 
ACTATCAACC GATGGCTTGC CGTCCGACTT GAATTCCTGG GTAGTTGCCT CATGTTCTCC 
ACCGCGCTCA CATCAGTCGC GGCTCTTATC ATGTCCAACA GTGTGGATGC GGGATTGGTC 
GGTCTCTTGA TGTCTTATAC TATTTCCGTC ACCGGTACTC TTAACTGGCT TGTGCGGAGC 
GCTTCCGAGG TGGAGCAGAA CATTGTGTCT GTCGAGCGTG TGCTTGGGTA TGCCAACTTG 
GACTCTGAAG CACCCGACTT TATCCCCGAG ACCAAGCCGG CTTCTACTTG GCCTCAAGAA 
GGATCGATCG AATTTGATCA CTTTAGTATG AAATACCGAC CGGAGCTTGA TTTTGTTCTT 
CGGGACGTTT GCATCAAGAT CAACGGTGGG GAGCGTGTCG GTGTCTGTGG CCGAACGGGT 
GCGGGCAAGT CATCGCTCAC CTTGGCACTT TTCCGCATCA TCGAAGCTGC AGGAGGCAAG 
ATTTTCATTG ACGGTGTCGA TATTTCGACG ATTGGCTTGC ACGACTTACG GACGATTGTC 
TCCATCATCC CGCAAGACCC TCAGTTGTTC GAAGGCACTT TGAGGAACAA TATCGATCCT 
ACCGAGTCCG CATCTGATGC CGATATGTGG CGAGCTCTTG AGCAAGCCCA CTTGAAGGAC 
CATGTCATGA ACAATATGGG TGGCTCGCTT GACGCCGAGG TTTCCGAGGG TGGTAGCAAC 
TTGAGTGCTG GTCAGCGTCA ACTCTTATGC TTTGCGCGCG CCATGCTACG CAAGACCAAG 
ATTCTAGTTT TGGACGAAGC TACTAGCTCT ATCGATCTTG AAACCGATGA GGCTGTTCAG 
CAAATCTTGC GTGGGCCTGA TTTCAAAGAT GTCACTACTA TCACCATTGC GCATCGGATC 
AACACGATCA TAGATAGTCA CCGCGTGCTT GTCATGTCTG AAGGCCGGGT GGCAGAGTAC 
GATACGCCGC AGGTACTCAT GCAGAGGCCT GAATCGTTGT TTTTCTCCTT GGTGCAGGAA 
GCTGGTCTGG AGAACGCAAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_569810.1
CDS	245..5008
Translation	MTAQAAFEAFASKHTRPYDCTFPPKIVSSHRDLDFTQCFEHAVLLPAPLAFFTLLALSQILRITRQLKKGGLNGGLAWITRSRRSERICSTKVGFLAASAVFSLVSLCLTFKNISVNLLSAAHYLLLFVTLLSLIHLTALNHHTSRTSSTIILLFYPTYLLVFAIRLRTMIVSGALNSGLTHSTSGRLLLARQAFWFASIIAALPAFLLELYSPEKKWQRWNAPWKRNGKIALEDDEEEEADGIENLDGQGAVPGKNAYGDVESPVSTANIYEILTFSWLTPLLSLGTRKYLGEEDMWALPSEDSAEALSNRLAETWKSQAEQVKAGKKKSASLKIALVKAYGGPYIVAGILKALYDMINFLQPQLLRLLLNFVSSYTSERPMPPVTGYAIAVLMFISANVGTAVLHQYFQRCFTTTMRIRGGLVTLIYRKALVLSNGEKSGRTTGDIVNLQSVDAVRIADVCQYGHIAWSGPFQILIAFISLYRLVGWQAFMGVAVMVVSLPANTLISRFNKRYHRRLMKVKDTRTRTMNEILNNIKSIKLYGWEQAFANKIYDIRNNQELKMLRKIGIVMAGSNFIWQGTPFLVAFSTFATFAFTSDKPLTSEIIFPAISLFQLLSFPMAMFANILNSIIEASVSVGRLESFLAADELNPNARTVIRPSEDPQGEPQKGDTVVSIKNGEFRWLEGSTEPILQDIDLEVKKGELIALIGRVGDGKSSLLGAILGEMTRSDGSVTLRGEVAYFSQNSWILSATVKDNIVFGHRFDKQFYEQVLDACALRQDLAVLPSGDMTEVGEKGVSLSGGQKARICLARAVYARADIYLLDDPLAAVDSHVGRHIFDKVIGPNGLLASKARILCTNAVTFLPQADQIISLRRGIVLERGTYEEAMNDSSSELYKLITGLGKQSAMGDGQDSGQGSGATTPTVVGQEEVVVVEEPEGVEDSEEAEIVTGADSPKQRKAYRQLSRDIMRRSSVVSLRTAKRDALRDLRESAKPKERSEKGNVKREVYREFIKASSKWGVAVFIGAMSLAQGLNILSNFVLRAWASANSDSSGEMPSVTKYLLIYGIVGISGSVANVVSVATLKIVCALKSSRSLHDRSFGALMKSPLSFFELTPTGRILNLFSRDIFVIDEVLIMALGGFFRTSVSVLGTVVVIAMGTPLVLLVFIPLGYLYRIVMRFYLATSRELKRLDAVSRSPVFSFFGETLSGLPVIRGYGQSARFIANNEARVDRNQACFMPAMTINRWLAVRLEFLGSCLMFSTALTSVAALIMSNSVDAGLVGLLMSYTISVTGTLNWLVRSASEVEQNIVSVERVLGYANLDSEAPDFIPETKPASTWPQEGSIEFDHFSMKYRPELDFVLRDVCIKINGGERVGVCGRTGAGKSSLTLALFRIIEAAGGKIFIDGVDISTIGLHDLRTIVSIIPQDPQLFEGTLRNNIDPTESASDADMWRALEQAHLKDHVMNNMGGSLDAEVSEGGSNLSAGQRQLLCFARAMLRKTKILVLDEATSSIDLETDEAVQQILRGPDFKDVTTITIAHRINTIIDSHRVLVMSEGRVAEYDTPQVLMQRPESLFFSLVQEAGLENAI

Target ORF information:

RefSeq Version	XM_569810.2
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 metal resistance protein ycf1, putative (CNC05860), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_569810.2

ORF Insert Sequence:

ATGACTGCCC AAGCAGCCTT CGAGGCCTTC GCCTCGAAGC ACACCCGCCC GTATGACTGT 
ACCTTTCCTC CAAAAATTGT CTCGTCCCAT AGGGATCTCG ACTTTACCCA GTGCTTCGAG 
CACGCCGTTT TACTGCCCGC CCCTCTCGCC TTCTTCACCT TGCTCGCACT TTCCCAGATC 
TTGAGAATCA CAAGACAACT CAAGAAAGGC GGACTGAACG GTGGTCTTGC ATGGATTACG 
AGGTCCAGGA GAAGTGAGAG AATATGCTCC ACCAAGGTGG GCTTCCTCGC AGCTTCTGCC 
GTCTTCTCCC TAGTTTCATT ATGCCTTACA TTCAAAAACA TCTCTGTAAA TCTTCTTTCC 
GCCGCCCACT ACTTGCTGCT CTTCGTGACT CTCTTATCGC TTATCCACCT CACCGCCTTG 
AACCACCACA CTTCCCGAAC TTCATCGACT ATTATTCTTC TCTTCTATCC CACCTATCTT 
CTCGTATTTG CCATCCGCCT TAGGACAATG ATCGTTTCTG GTGCCCTCAA CTCTGGTCTT 
ACCCATTCTA CTTCAGGGCG CTTGTTGTTG GCAAGACAGG CTTTCTGGTT CGCAAGTATC 
ATTGCGGCTC TTCCCGCTTT TCTGCTCGAA CTATACTCTC CTGAAAAGAA ATGGCAGAGA 
TGGAATGCTC CTTGGAAGAG AAACGGCAAA ATCGCCTTGG AGGATGACGA GGAAGAAGAA 
GCGGATGGAA TCGAGAACTT GGACGGCCAA GGTGCTGTGC CGGGCAAGAA CGCTTACGGC 
GATGTCGAGA GCCCCGTTTC CACCGCCAAT ATCTATGAAA TTCTGACTTT TTCTTGGCTC 
ACTCCTCTTC TCTCTCTTGG TACCCGCAAG TACCTTGGTG AAGAAGACAT GTGGGCGCTC 
CCCTCTGAAG ACTCTGCCGA AGCTCTTTCC AACCGTCTTG CGGAAACCTG GAAATCGCAA 
GCTGAACAGG TCAAAGCTGG CAAAAAGAAG AGCGCTTCTC TCAAGATCGC CCTTGTCAAG 
GCTTACGGTG GTCCTTATAT TGTTGCTGGT ATCCTCAAGG CCTTGTATGA TATGATCAAC 
TTTCTGCAGC CTCAACTTTT GAGGTTGTTA CTCAACTTTG TCTCGAGCTA CACTAGTGAA 
CGTCCAATGC CGCCTGTTAC TGGGTACGCT ATTGCCGTTT TGATGTTTAT CAGCGCAAAC 
GTAGGAACCG CCGTTTTGCA CCAGTACTTC CAGCGATGCT TTACCACCAC TATGCGTATC 
CGGGGTGGAC TTGTCACTTT GATTTACCGC AAGGCACTTG TCCTTAGCAA CGGCGAAAAG 
TCTGGCAGGA CTACTGGTGA CATTGTCAAC CTCCAGAGCG TTGATGCCGT CAGGATTGCA 
GATGTCTGTC AGTACGGTCA TATTGCTTGG TCAGGCCCTT TCCAGATTCT CATTGCATTC 
ATTTCTCTCT ACAGGCTTGT CGGCTGGCAA GCGTTTATGG GCGTTGCTGT CATGGTGGTC 
AGTTTGCCAG CAAACACTCT CATTTCTCGA TTCAATAAGC GCTACCACCG GCGATTAATG 
AAGGTCAAGG ACACTCGTAC TCGTACCATG AACGAGATCC TCAACAACAT CAAGTCTATC 
AAGCTGTACG GCTGGGAGCA GGCATTTGCC AACAAGATCT ACGACATCCG TAACAACCAA 
GAACTCAAGA TGTTGCGCAA GATTGGTATC GTCATGGCTG GAAGTAACTT TATCTGGCAA 
GGTACCCCCT TCCTTGTTGC ATTCTCTACC TTTGCCACGT TTGCTTTCAC CAGCGACAAA 
CCTTTGACCA GTGAGATCAT TTTCCCCGCC ATCTCTCTCT TCCAGCTTCT CTCCTTCCCC 
ATGGCCATGT TTGCCAACAT CCTCAACTCT ATCATCGAAG CTTCCGTTTC AGTCGGCCGT 
TTGGAAAGTT TCCTTGCTGC TGATGAACTC AACCCTAATG CCCGCACTGT CATTCGACCT 
TCTGAAGATC CTCAGGGTGA GCCTCAAAAG GGCGATACCG TTGTTAGCAT TAAGAATGGC 
GAATTCCGAT GGCTCGAGGG CTCTACGGAG CCCATCCTGC AGGACATCGA CCTTGAAGTT 
AAAAAGGGTG AGCTTATCGC TCTTATCGGC CGAGTTGGTG ACGGCAAATC ATCTCTGTTG 
GGCGCGATTC TTGGTGAAAT GACTCGCTCT GACGGCTCTG TCACTCTCCG CGGCGAGGTC 
GCATACTTTT CGCAAAACTC TTGGATCCTC TCTGCGACTG TCAAGGATAA CATTGTGTTT 
GGCCACCGAT TCGACAAACA GTTTTACGAA CAGGTGTTGG ATGCTTGTGC TTTGAGGCAA 
GACCTTGCGG TACTTCCTAG CGGCGATATG ACCGAGGTCG GTGAAAAGGG TGTTTCCCTC 
TCTGGTGGTC AAAAGGCTCG TATCTGTCTT GCTAGAGCTG TCTATGCTCG TGCCGATATT 
TACCTTCTTG ACGATCCTCT TGCGGCCGTT GATTCTCATG TCGGTCGACA CATTTTCGAC 
AAGGTCATCG GGCCTAACGG TCTCCTTGCT TCAAAAGCCC GAATTCTCTG TACCAACGCT 
GTCACTTTTC TTCCCCAGGC CGACCAAATC ATCTCTCTCC GTAGAGGTAT TGTGCTTGAG 
CGGGGTACTT ATGAAGAGGC CATGAACGAT TCGTCCTCTG AGCTTTACAA GCTTATCACT 
GGTTTGGGCA AGCAATCTGC CATGGGCGAT GGACAAGATT CTGGACAAGG TTCTGGTGCT 
ACTACACCTA CGGTTGTTGG ACAAGAGGAG GTGGTGGTAG TTGAGGAACC AGAGGGTGTG 
GAAGACAGCG AAGAAGCTGA GATTGTCACT GGCGCTGATT CTCCTAAACA ACGCAAAGCG 
TACCGACAGC TCAGCCGCGA CATCATGCGT CGATCTTCTG TCGTTTCTCT CCGTACTGCC 
AAACGTGACG CCCTGCGTGA CCTACGCGAA AGCGCTAAAC CAAAGGAACG CTCTGAAAAG 
GGCAACGTGA AGCGAGAAGT CTACCGGGAA TTCATCAAGG CCTCTTCCAA ATGGGGCGTT 
GCCGTCTTTA TCGGCGCGAT GAGTCTCGCG CAGGGTTTGA ACATTCTTTC CAACTTTGTA 
CTTCGTGCTT GGGCCAGTGC CAACTCTGAT AGTTCAGGCG AGATGCCGAG TGTGACCAAA 
TACCTCCTCA TCTACGGTAT CGTCGGTATT TCTGGTTCCG TGGCCAACGT TGTCAGCGTC 
GCCACGCTCA AGATTGTTTG TGCCCTCAAG TCTAGTAGGA GTCTCCATGA CAGGTCATTT 
GGGGCTTTGA TGAAGAGTCC CTTGTCATTC TTCGAGCTTA CACCAACAGG ACGAATCCTC 
AACTTGTTCT CTAGGGATAT CTTTGTTATT GATGAAGTCT TGATTATGGC TCTCGGTGGT 
TTCTTCCGTA CTTCCGTATC AGTCCTCGGT ACTGTGGTAG TCATTGCTAT GGGCACGCCT 
TTGGTGTTGC TCGTGTTCAT CCCTCTTGGT TACCTCTACA GGATTGTCAT GAGATTCTAT 
CTTGCTACTT CTCGAGAGCT GAAACGGCTT GACGCCGTTT CTCGATCTCC CGTCTTCTCC 
TTCTTTGGCG AAACCCTTTC CGGTCTGCCT GTTATCCGAG GATATGGCCA ATCTGCCCGA 
TTCATTGCCA ACAACGAAGC CCGTGTGGAT CGAAACCAGG CGTGTTTCAT GCCGGCTATG 
ACTATCAACC GATGGCTTGC CGTCCGACTT GAATTCCTGG GTAGTTGCCT CATGTTCTCC 
ACCGCGCTCA CATCAGTCGC GGCTCTTATC ATGTCCAACA GTGTGGATGC GGGATTGGTC 
GGTCTCTTGA TGTCTTATAC TATTTCCGTC ACCGGTACTC TTAACTGGCT TGTGCGGAGC 
GCTTCCGAGG TGGAGCAGAA CATTGTGTCT GTCGAGCGTG TGCTTGGGTA TGCCAACTTG 
GACTCTGAAG CACCCGACTT TATCCCCGAG ACCAAGCCGG CTTCTACTTG GCCTCAAGAA 
GGATCGATCG AATTTGATCA CTTTAGTATG AAATACCGAC CGGAGCTTGA TTTTGTTCTT 
CGGGACGTTT GCATCAAGAT CAACGGTGGG GAGCGTGTCG GTGTCTGTGG CCGAACGGGT 
GCGGGCAAGT CATCGCTCAC CTTGGCACTT TTCCGCATCA TCGAAGCTGC AGGAGGCAAG 
ATTTTCATTG ACGGTGTCGA TATTTCGACG ATTGGCTTGC ACGACTTACG GACGATTGTC 
TCCATCATCC CGCAAGACCC TCAGTTGTTC GAAGGCACTT TGAGGAACAA TATCGATCCT 
ACCGAGTCCG CATCTGATGC CGATATGTGG CGAGCTCTTG AGCAAGCCCA CTTGAAGGAC 
CATGTCATGA ACAATATGGG TGGCTCGCTT GACGCCGAGG TTTCCGAGGG TGGTAGCAAC 
TTGAGTGCTG GTCAGCGTCA ACTCTTATGC TTTGCGCGCG CCATGCTACG CAAGACCAAG 
ATTCTAGTTT TGGACGAAGC TACTAGCTCT ATCGATCTTG AAACCGATGA GGCTGTTCAG 
CAAATCTTGC GTGGGCCTGA TTTCAAAGAT GTCACTACTA TCACCATTGC GCATCGGATC 
AACACGATCA TAGATAGTCA CCGCGTGCTT GTCATGTCTG AAGGCCGGGT GGCAGAGTAC 
GATACGCCGC AGGTACTCAT GCAGAGGCCT GAATCGTTGT TTTTCTCCTT GGTGCAGGAA 
GCTGGTCTGG AGAACGCAAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNC05860 ( XM_569810.1 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_569810.1 Cryptococcus neoformans var. neoformans JEC21 metal resistance protein ycf1, partial mRNA.

Price & Availability↑

CloneID	OCn03456
Clone ID Related Accession (Same CDS sequence)	XM_569810.2 , XM_569810.1
Accession Version	XM_569810.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1345046400000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	metal resistance protein ycf1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006685). The reference sequence was derived from mrna.CNC05860.1. This record represents the complete annotated sequence of the C. neoformans JEC21 genome. A small number of gaps exist which are represented by NNNNs within the final sequence. The genome has been annotated by TIGR and the annotation data is available for viewing and download from the TIGR database (http://www.tigr.org/tdb/e2k1/cna1/). COMPLETENESS: incomplete on the 5' end.

ATGACTGCCC AAGCAGCCTT CGAGGCCTTC GCCTCGAAGC ACACCCGCCC GTATGACTGT 
ACCTTTCCTC CAAAAATTGT CTCGTCCCAT AGGGATCTCG ACTTTACCCA GTGCTTCGAG 
CACGCCGTTT TACTGCCCGC CCCTCTCGCC TTCTTCACCT TGCTCGCACT TTCCCAGATC 
TTGAGAATCA CAAGACAACT CAAGAAAGGC GGACTGAACG GTGGTCTTGC ATGGATTACG 
AGGTCCAGGA GAAGTGAGAG AATATGCTCC ACCAAGGTGG GCTTCCTCGC AGCTTCTGCC 
GTCTTCTCCC TAGTTTCATT ATGCCTTACA TTCAAAAACA TCTCTGTAAA TCTTCTTTCC 
GCCGCCCACT ACTTGCTGCT CTTCGTGACT CTCTTATCGC TTATCCACCT CACCGCCTTG 
AACCACCACA CTTCCCGAAC TTCATCGACT ATTATTCTTC TCTTCTATCC CACCTATCTT 
CTCGTATTTG CCATCCGCCT TAGGACAATG ATCGTTTCTG GTGCCCTCAA CTCTGGTCTT 
ACCCATTCTA CTTCAGGGCG CTTGTTGTTG GCAAGACAGG CTTTCTGGTT CGCAAGTATC 
ATTGCGGCTC TTCCCGCTTT TCTGCTCGAA CTATACTCTC CTGAAAAGAA ATGGCAGAGA 
TGGAATGCTC CTTGGAAGAG AAACGGCAAA ATCGCCTTGG AGGATGACGA GGAAGAAGAA 
GCGGATGGAA TCGAGAACTT GGACGGCCAA GGTGCTGTGC CGGGCAAGAA CGCTTACGGC 
GATGTCGAGA GCCCCGTTTC CACCGCCAAT ATCTATGAAA TTCTGACTTT TTCTTGGCTC 
ACTCCTCTTC TCTCTCTTGG TACCCGCAAG TACCTTGGTG AAGAAGACAT GTGGGCGCTC 
CCCTCTGAAG ACTCTGCCGA AGCTCTTTCC AACCGTCTTG CGGAAACCTG GAAATCGCAA 
GCTGAACAGG TCAAAGCTGG CAAAAAGAAG AGCGCTTCTC TCAAGATCGC CCTTGTCAAG 
GCTTACGGTG GTCCTTATAT TGTTGCTGGT ATCCTCAAGG CCTTGTATGA TATGATCAAC 
TTTCTGCAGC CTCAACTTTT GAGGTTGTTA CTCAACTTTG TCTCGAGCTA CACTAGTGAA 
CGTCCAATGC CGCCTGTTAC TGGGTACGCT ATTGCCGTTT TGATGTTTAT CAGCGCAAAC 
GTAGGAACCG CCGTTTTGCA CCAGTACTTC CAGCGATGCT TTACCACCAC TATGCGTATC 
CGGGGTGGAC TTGTCACTTT GATTTACCGC AAGGCACTTG TCCTTAGCAA CGGCGAAAAG 
TCTGGCAGGA CTACTGGTGA CATTGTCAAC CTCCAGAGCG TTGATGCCGT CAGGATTGCA 
GATGTCTGTC AGTACGGTCA TATTGCTTGG TCAGGCCCTT TCCAGATTCT CATTGCATTC 
ATTTCTCTCT ACAGGCTTGT CGGCTGGCAA GCGTTTATGG GCGTTGCTGT CATGGTGGTC 
AGTTTGCCAG CAAACACTCT CATTTCTCGA TTCAATAAGC GCTACCACCG GCGATTAATG 
AAGGTCAAGG ACACTCGTAC TCGTACCATG AACGAGATCC TCAACAACAT CAAGTCTATC 
AAGCTGTACG GCTGGGAGCA GGCATTTGCC AACAAGATCT ACGACATCCG TAACAACCAA 
GAACTCAAGA TGTTGCGCAA GATTGGTATC GTCATGGCTG GAAGTAACTT TATCTGGCAA 
GGTACCCCCT TCCTTGTTGC ATTCTCTACC TTTGCCACGT TTGCTTTCAC CAGCGACAAA 
CCTTTGACCA GTGAGATCAT TTTCCCCGCC ATCTCTCTCT TCCAGCTTCT CTCCTTCCCC 
ATGGCCATGT TTGCCAACAT CCTCAACTCT ATCATCGAAG CTTCCGTTTC AGTCGGCCGT 
TTGGAAAGTT TCCTTGCTGC TGATGAACTC AACCCTAATG CCCGCACTGT CATTCGACCT 
TCTGAAGATC CTCAGGGTGA GCCTCAAAAG GGCGATACCG TTGTTAGCAT TAAGAATGGC 
GAATTCCGAT GGCTCGAGGG CTCTACGGAG CCCATCCTGC AGGACATCGA CCTTGAAGTT 
AAAAAGGGTG AGCTTATCGC TCTTATCGGC CGAGTTGGTG ACGGCAAATC ATCTCTGTTG 
GGCGCGATTC TTGGTGAAAT GACTCGCTCT GACGGCTCTG TCACTCTCCG CGGCGAGGTC 
GCATACTTTT CGCAAAACTC TTGGATCCTC TCTGCGACTG TCAAGGATAA CATTGTGTTT 
GGCCACCGAT TCGACAAACA GTTTTACGAA CAGGTGTTGG ATGCTTGTGC TTTGAGGCAA 
GACCTTGCGG TACTTCCTAG CGGCGATATG ACCGAGGTCG GTGAAAAGGG TGTTTCCCTC 
TCTGGTGGTC AAAAGGCTCG TATCTGTCTT GCTAGAGCTG TCTATGCTCG TGCCGATATT 
TACCTTCTTG ACGATCCTCT TGCGGCCGTT GATTCTCATG TCGGTCGACA CATTTTCGAC 
AAGGTCATCG GGCCTAACGG TCTCCTTGCT TCAAAAGCCC GAATTCTCTG TACCAACGCT 
GTCACTTTTC TTCCCCAGGC CGACCAAATC ATCTCTCTCC GTAGAGGTAT TGTGCTTGAG 
CGGGGTACTT ATGAAGAGGC CATGAACGAT TCGTCCTCTG AGCTTTACAA GCTTATCACT 
GGTTTGGGCA AGCAATCTGC CATGGGCGAT GGACAAGATT CTGGACAAGG TTCTGGTGCT 
ACTACACCTA CGGTTGTTGG ACAAGAGGAG GTGGTGGTAG TTGAGGAACC AGAGGGTGTG 
GAAGACAGCG AAGAAGCTGA GATTGTCACT GGCGCTGATT CTCCTAAACA ACGCAAAGCG 
TACCGACAGC TCAGCCGCGA CATCATGCGT CGATCTTCTG TCGTTTCTCT CCGTACTGCC 
AAACGTGACG CCCTGCGTGA CCTACGCGAA AGCGCTAAAC CAAAGGAACG CTCTGAAAAG 
GGCAACGTGA AGCGAGAAGT CTACCGGGAA TTCATCAAGG CCTCTTCCAA ATGGGGCGTT 
GCCGTCTTTA TCGGCGCGAT GAGTCTCGCG CAGGGTTTGA ACATTCTTTC CAACTTTGTA 
CTTCGTGCTT GGGCCAGTGC CAACTCTGAT AGTTCAGGCG AGATGCCGAG TGTGACCAAA 
TACCTCCTCA TCTACGGTAT CGTCGGTATT TCTGGTTCCG TGGCCAACGT TGTCAGCGTC 
GCCACGCTCA AGATTGTTTG TGCCCTCAAG TCTAGTAGGA GTCTCCATGA CAGGTCATTT 
GGGGCTTTGA TGAAGAGTCC CTTGTCATTC TTCGAGCTTA CACCAACAGG ACGAATCCTC 
AACTTGTTCT CTAGGGATAT CTTTGTTATT GATGAAGTCT TGATTATGGC TCTCGGTGGT 
TTCTTCCGTA CTTCCGTATC AGTCCTCGGT ACTGTGGTAG TCATTGCTAT GGGCACGCCT 
TTGGTGTTGC TCGTGTTCAT CCCTCTTGGT TACCTCTACA GGATTGTCAT GAGATTCTAT 
CTTGCTACTT CTCGAGAGCT GAAACGGCTT GACGCCGTTT CTCGATCTCC CGTCTTCTCC 
TTCTTTGGCG AAACCCTTTC CGGTCTGCCT GTTATCCGAG GATATGGCCA ATCTGCCCGA 
TTCATTGCCA ACAACGAAGC CCGTGTGGAT CGAAACCAGG CGTGTTTCAT GCCGGCTATG 
ACTATCAACC GATGGCTTGC CGTCCGACTT GAATTCCTGG GTAGTTGCCT CATGTTCTCC 
ACCGCGCTCA CATCAGTCGC GGCTCTTATC ATGTCCAACA GTGTGGATGC GGGATTGGTC 
GGTCTCTTGA TGTCTTATAC TATTTCCGTC ACCGGTACTC TTAACTGGCT TGTGCGGAGC 
GCTTCCGAGG TGGAGCAGAA CATTGTGTCT GTCGAGCGTG TGCTTGGGTA TGCCAACTTG 
GACTCTGAAG CACCCGACTT TATCCCCGAG ACCAAGCCGG CTTCTACTTG GCCTCAAGAA 
GGATCGATCG AATTTGATCA CTTTAGTATG AAATACCGAC CGGAGCTTGA TTTTGTTCTT 
CGGGACGTTT GCATCAAGAT CAACGGTGGG GAGCGTGTCG GTGTCTGTGG CCGAACGGGT 
GCGGGCAAGT CATCGCTCAC CTTGGCACTT TTCCGCATCA TCGAAGCTGC AGGAGGCAAG 
ATTTTCATTG ACGGTGTCGA TATTTCGACG ATTGGCTTGC ACGACTTACG GACGATTGTC 
TCCATCATCC CGCAAGACCC TCAGTTGTTC GAAGGCACTT TGAGGAACAA TATCGATCCT 
ACCGAGTCCG CATCTGATGC CGATATGTGG CGAGCTCTTG AGCAAGCCCA CTTGAAGGAC 
CATGTCATGA ACAATATGGG TGGCTCGCTT GACGCCGAGG TTTCCGAGGG TGGTAGCAAC 
TTGAGTGCTG GTCAGCGTCA ACTCTTATGC TTTGCGCGCG CCATGCTACG CAAGACCAAG 
ATTCTAGTTT TGGACGAAGC TACTAGCTCT ATCGATCTTG AAACCGATGA GGCTGTTCAG 
CAAATCTTGC GTGGGCCTGA TTTCAAAGAT GTCACTACTA TCACCATTGC GCATCGGATC 
AACACGATCA TAGATAGTCA CCGCGTGCTT GTCATGTCTG AAGGCCGGGT GGCAGAGTAC 
GATACGCCGC AGGTACTCAT GCAGAGGCCT GAATCGTTGT TTTTCTCCTT GGTGCAGGAA 
GCTGGTCTGG AGAACGCAAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_569810.1
CDS	1..4764
Translation	MTAQAAFEAFASKHTRPYDCTFPPKIVSSHRDLDFTQCFEHAVLLPAPLAFFTLLALSQILRITRQLKKGGLNGGLAWITRSRRSERICSTKVGFLAASAVFSLVSLCLTFKNISVNLLSAAHYLLLFVTLLSLIHLTALNHHTSRTSSTIILLFYPTYLLVFAIRLRTMIVSGALNSGLTHSTSGRLLLARQAFWFASIIAALPAFLLELYSPEKKWQRWNAPWKRNGKIALEDDEEEEADGIENLDGQGAVPGKNAYGDVESPVSTANIYEILTFSWLTPLLSLGTRKYLGEEDMWALPSEDSAEALSNRLAETWKSQAEQVKAGKKKSASLKIALVKAYGGPYIVAGILKALYDMINFLQPQLLRLLLNFVSSYTSERPMPPVTGYAIAVLMFISANVGTAVLHQYFQRCFTTTMRIRGGLVTLIYRKALVLSNGEKSGRTTGDIVNLQSVDAVRIADVCQYGHIAWSGPFQILIAFISLYRLVGWQAFMGVAVMVVSLPANTLISRFNKRYHRRLMKVKDTRTRTMNEILNNIKSIKLYGWEQAFANKIYDIRNNQELKMLRKIGIVMAGSNFIWQGTPFLVAFSTFATFAFTSDKPLTSEIIFPAISLFQLLSFPMAMFANILNSIIEASVSVGRLESFLAADELNPNARTVIRPSEDPQGEPQKGDTVVSIKNGEFRWLEGSTEPILQDIDLEVKKGELIALIGRVGDGKSSLLGAILGEMTRSDGSVTLRGEVAYFSQNSWILSATVKDNIVFGHRFDKQFYEQVLDACALRQDLAVLPSGDMTEVGEKGVSLSGGQKARICLARAVYARADIYLLDDPLAAVDSHVGRHIFDKVIGPNGLLASKARILCTNAVTFLPQADQIISLRRGIVLERGTYEEAMNDSSSELYKLITGLGKQSAMGDGQDSGQGSGATTPTVVGQEEVVVVEEPEGVEDSEEAEIVTGADSPKQRKAYRQLSRDIMRRSSVVSLRTAKRDALRDLRESAKPKERSEKGNVKREVYREFIKASSKWGVAVFIGAMSLAQGLNILSNFVLRAWASANSDSSGEMPSVTKYLLIYGIVGISGSVANVVSVATLKIVCALKSSRSLHDRSFGALMKSPLSFFELTPTGRILNLFSRDIFVIDEVLIMALGGFFRTSVSVLGTVVVIAMGTPLVLLVFIPLGYLYRIVMRFYLATSRELKRLDAVSRSPVFSFFGETLSGLPVIRGYGQSARFIANNEARVDRNQACFMPAMTINRWLAVRLEFLGSCLMFSTALTSVAALIMSNSVDAGLVGLLMSYTISVTGTLNWLVRSASEVEQNIVSVERVLGYANLDSEAPDFIPETKPASTWPQEGSIEFDHFSMKYRPELDFVLRDVCIKINGGERVGVCGRTGAGKSSLTLALFRIIEAAGGKIFIDGVDISTIGLHDLRTIVSIIPQDPQLFEGTLRNNIDPTESASDADMWRALEQAHLKDHVMNNMGGSLDAEVSEGGSNLSAGQRQLLCFARAMLRKTKILVLDEATSSIDLETDEAVQQILRGPDFKDVTTITIAHRINTIIDSHRVLVMSEGRVAEYDTPQVLMQRPESLFFSLVQEAGLENAI

Target ORF information:

RefSeq Version	XM_569810.1
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 metal resistance protein ycf1, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_569810.1

ORF Insert Sequence:

ATGACTGCCC AAGCAGCCTT CGAGGCCTTC GCCTCGAAGC ACACCCGCCC GTATGACTGT 
ACCTTTCCTC CAAAAATTGT CTCGTCCCAT AGGGATCTCG ACTTTACCCA GTGCTTCGAG 
CACGCCGTTT TACTGCCCGC CCCTCTCGCC TTCTTCACCT TGCTCGCACT TTCCCAGATC 
TTGAGAATCA CAAGACAACT CAAGAAAGGC GGACTGAACG GTGGTCTTGC ATGGATTACG 
AGGTCCAGGA GAAGTGAGAG AATATGCTCC ACCAAGGTGG GCTTCCTCGC AGCTTCTGCC 
GTCTTCTCCC TAGTTTCATT ATGCCTTACA TTCAAAAACA TCTCTGTAAA TCTTCTTTCC 
GCCGCCCACT ACTTGCTGCT CTTCGTGACT CTCTTATCGC TTATCCACCT CACCGCCTTG 
AACCACCACA CTTCCCGAAC TTCATCGACT ATTATTCTTC TCTTCTATCC CACCTATCTT 
CTCGTATTTG CCATCCGCCT TAGGACAATG ATCGTTTCTG GTGCCCTCAA CTCTGGTCTT 
ACCCATTCTA CTTCAGGGCG CTTGTTGTTG GCAAGACAGG CTTTCTGGTT CGCAAGTATC 
ATTGCGGCTC TTCCCGCTTT TCTGCTCGAA CTATACTCTC CTGAAAAGAA ATGGCAGAGA 
TGGAATGCTC CTTGGAAGAG AAACGGCAAA ATCGCCTTGG AGGATGACGA GGAAGAAGAA 
GCGGATGGAA TCGAGAACTT GGACGGCCAA GGTGCTGTGC CGGGCAAGAA CGCTTACGGC 
GATGTCGAGA GCCCCGTTTC CACCGCCAAT ATCTATGAAA TTCTGACTTT TTCTTGGCTC 
ACTCCTCTTC TCTCTCTTGG TACCCGCAAG TACCTTGGTG AAGAAGACAT GTGGGCGCTC 
CCCTCTGAAG ACTCTGCCGA AGCTCTTTCC AACCGTCTTG CGGAAACCTG GAAATCGCAA 
GCTGAACAGG TCAAAGCTGG CAAAAAGAAG AGCGCTTCTC TCAAGATCGC CCTTGTCAAG 
GCTTACGGTG GTCCTTATAT TGTTGCTGGT ATCCTCAAGG CCTTGTATGA TATGATCAAC 
TTTCTGCAGC CTCAACTTTT GAGGTTGTTA CTCAACTTTG TCTCGAGCTA CACTAGTGAA 
CGTCCAATGC CGCCTGTTAC TGGGTACGCT ATTGCCGTTT TGATGTTTAT CAGCGCAAAC 
GTAGGAACCG CCGTTTTGCA CCAGTACTTC CAGCGATGCT TTACCACCAC TATGCGTATC 
CGGGGTGGAC TTGTCACTTT GATTTACCGC AAGGCACTTG TCCTTAGCAA CGGCGAAAAG 
TCTGGCAGGA CTACTGGTGA CATTGTCAAC CTCCAGAGCG TTGATGCCGT CAGGATTGCA 
GATGTCTGTC AGTACGGTCA TATTGCTTGG TCAGGCCCTT TCCAGATTCT CATTGCATTC 
ATTTCTCTCT ACAGGCTTGT CGGCTGGCAA GCGTTTATGG GCGTTGCTGT CATGGTGGTC 
AGTTTGCCAG CAAACACTCT CATTTCTCGA TTCAATAAGC GCTACCACCG GCGATTAATG 
AAGGTCAAGG ACACTCGTAC TCGTACCATG AACGAGATCC TCAACAACAT CAAGTCTATC 
AAGCTGTACG GCTGGGAGCA GGCATTTGCC AACAAGATCT ACGACATCCG TAACAACCAA 
GAACTCAAGA TGTTGCGCAA GATTGGTATC GTCATGGCTG GAAGTAACTT TATCTGGCAA 
GGTACCCCCT TCCTTGTTGC ATTCTCTACC TTTGCCACGT TTGCTTTCAC CAGCGACAAA 
CCTTTGACCA GTGAGATCAT TTTCCCCGCC ATCTCTCTCT TCCAGCTTCT CTCCTTCCCC 
ATGGCCATGT TTGCCAACAT CCTCAACTCT ATCATCGAAG CTTCCGTTTC AGTCGGCCGT 
TTGGAAAGTT TCCTTGCTGC TGATGAACTC AACCCTAATG CCCGCACTGT CATTCGACCT 
TCTGAAGATC CTCAGGGTGA GCCTCAAAAG GGCGATACCG TTGTTAGCAT TAAGAATGGC 
GAATTCCGAT GGCTCGAGGG CTCTACGGAG CCCATCCTGC AGGACATCGA CCTTGAAGTT 
AAAAAGGGTG AGCTTATCGC TCTTATCGGC CGAGTTGGTG ACGGCAAATC ATCTCTGTTG 
GGCGCGATTC TTGGTGAAAT GACTCGCTCT GACGGCTCTG TCACTCTCCG CGGCGAGGTC 
GCATACTTTT CGCAAAACTC TTGGATCCTC TCTGCGACTG TCAAGGATAA CATTGTGTTT 
GGCCACCGAT TCGACAAACA GTTTTACGAA CAGGTGTTGG ATGCTTGTGC TTTGAGGCAA 
GACCTTGCGG TACTTCCTAG CGGCGATATG ACCGAGGTCG GTGAAAAGGG TGTTTCCCTC 
TCTGGTGGTC AAAAGGCTCG TATCTGTCTT GCTAGAGCTG TCTATGCTCG TGCCGATATT 
TACCTTCTTG ACGATCCTCT TGCGGCCGTT GATTCTCATG TCGGTCGACA CATTTTCGAC 
AAGGTCATCG GGCCTAACGG TCTCCTTGCT TCAAAAGCCC GAATTCTCTG TACCAACGCT 
GTCACTTTTC TTCCCCAGGC CGACCAAATC ATCTCTCTCC GTAGAGGTAT TGTGCTTGAG 
CGGGGTACTT ATGAAGAGGC CATGAACGAT TCGTCCTCTG AGCTTTACAA GCTTATCACT 
GGTTTGGGCA AGCAATCTGC CATGGGCGAT GGACAAGATT CTGGACAAGG TTCTGGTGCT 
ACTACACCTA CGGTTGTTGG ACAAGAGGAG GTGGTGGTAG TTGAGGAACC AGAGGGTGTG 
GAAGACAGCG AAGAAGCTGA GATTGTCACT GGCGCTGATT CTCCTAAACA ACGCAAAGCG 
TACCGACAGC TCAGCCGCGA CATCATGCGT CGATCTTCTG TCGTTTCTCT CCGTACTGCC 
AAACGTGACG CCCTGCGTGA CCTACGCGAA AGCGCTAAAC CAAAGGAACG CTCTGAAAAG 
GGCAACGTGA AGCGAGAAGT CTACCGGGAA TTCATCAAGG CCTCTTCCAA ATGGGGCGTT 
GCCGTCTTTA TCGGCGCGAT GAGTCTCGCG CAGGGTTTGA ACATTCTTTC CAACTTTGTA 
CTTCGTGCTT GGGCCAGTGC CAACTCTGAT AGTTCAGGCG AGATGCCGAG TGTGACCAAA 
TACCTCCTCA TCTACGGTAT CGTCGGTATT TCTGGTTCCG TGGCCAACGT TGTCAGCGTC 
GCCACGCTCA AGATTGTTTG TGCCCTCAAG TCTAGTAGGA GTCTCCATGA CAGGTCATTT 
GGGGCTTTGA TGAAGAGTCC CTTGTCATTC TTCGAGCTTA CACCAACAGG ACGAATCCTC 
AACTTGTTCT CTAGGGATAT CTTTGTTATT GATGAAGTCT TGATTATGGC TCTCGGTGGT 
TTCTTCCGTA CTTCCGTATC AGTCCTCGGT ACTGTGGTAG TCATTGCTAT GGGCACGCCT 
TTGGTGTTGC TCGTGTTCAT CCCTCTTGGT TACCTCTACA GGATTGTCAT GAGATTCTAT 
CTTGCTACTT CTCGAGAGCT GAAACGGCTT GACGCCGTTT CTCGATCTCC CGTCTTCTCC 
TTCTTTGGCG AAACCCTTTC CGGTCTGCCT GTTATCCGAG GATATGGCCA ATCTGCCCGA 
TTCATTGCCA ACAACGAAGC CCGTGTGGAT CGAAACCAGG CGTGTTTCAT GCCGGCTATG 
ACTATCAACC GATGGCTTGC CGTCCGACTT GAATTCCTGG GTAGTTGCCT CATGTTCTCC 
ACCGCGCTCA CATCAGTCGC GGCTCTTATC ATGTCCAACA GTGTGGATGC GGGATTGGTC 
GGTCTCTTGA TGTCTTATAC TATTTCCGTC ACCGGTACTC TTAACTGGCT TGTGCGGAGC 
GCTTCCGAGG TGGAGCAGAA CATTGTGTCT GTCGAGCGTG TGCTTGGGTA TGCCAACTTG 
GACTCTGAAG CACCCGACTT TATCCCCGAG ACCAAGCCGG CTTCTACTTG GCCTCAAGAA 
GGATCGATCG AATTTGATCA CTTTAGTATG AAATACCGAC CGGAGCTTGA TTTTGTTCTT 
CGGGACGTTT GCATCAAGAT CAACGGTGGG GAGCGTGTCG GTGTCTGTGG CCGAACGGGT 
GCGGGCAAGT CATCGCTCAC CTTGGCACTT TTCCGCATCA TCGAAGCTGC AGGAGGCAAG 
ATTTTCATTG ACGGTGTCGA TATTTCGACG ATTGGCTTGC ACGACTTACG GACGATTGTC 
TCCATCATCC CGCAAGACCC TCAGTTGTTC GAAGGCACTT TGAGGAACAA TATCGATCCT 
ACCGAGTCCG CATCTGATGC CGATATGTGG CGAGCTCTTG AGCAAGCCCA CTTGAAGGAC 
CATGTCATGA ACAATATGGG TGGCTCGCTT GACGCCGAGG TTTCCGAGGG TGGTAGCAAC 
TTGAGTGCTG GTCAGCGTCA ACTCTTATGC TTTGCGCGCG CCATGCTACG CAAGACCAAG 
ATTCTAGTTT TGGACGAAGC TACTAGCTCT ATCGATCTTG AAACCGATGA GGCTGTTCAG 
CAAATCTTGC GTGGGCCTGA TTTCAAAGAT GTCACTACTA TCACCATTGC GCATCGGATC 
AACACGATCA TAGATAGTCA CCGCGTGCTT GTCATGTCTG AAGGCCGGGT GGCAGAGTAC 
GATACGCCGC AGGTACTCAT GCAGAGGCCT GAATCGTTGT TTTTCTCCTT GGTGCAGGAA 
GCTGGTCTGG AGAACGCAAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)
Loftus BJ,Fung E,Roncaglia P,Rowley D,Amedeo P,Bruno D,Vamathevan J,Miranda M,Anderson IJ,Fraser JA,Allen JE,Bosdet IE,Brent MR,Chiu R,Doering TL,Donlin MJ,D'Souza CA,Fox DS,Grinberg V,Fu J,Fukushima M,Haas BJ,Huang JC,Janbon G,Jones SJ,Koo HL,Krzywinski MI,Kwon-Chung JK,Lengeler KB,Maiti R,Marra MA,Marra RE,Mathewson CA,Mitchell TG,Pertea M,Riggs FR,Salzberg SL,Schein JE,Shvartsbeyn A,Shin H,Shumway M,Specht CA,Suh BB,Tenney A,Utterback TR,Wickes BL,Wortman JR,Wye NH,Kronstad JW,Lodge JK,Heitman J,Davis RW,Fraser CM,Hyman RW

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CNC05860 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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